WO2019207644A1 - Fluid device, valve device, and detection device - Google Patents

Fluid device, valve device, and detection device Download PDF

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Publication number
WO2019207644A1
WO2019207644A1 PCT/JP2018/016606 JP2018016606W WO2019207644A1 WO 2019207644 A1 WO2019207644 A1 WO 2019207644A1 JP 2018016606 W JP2018016606 W JP 2018016606W WO 2019207644 A1 WO2019207644 A1 WO 2019207644A1
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WO
WIPO (PCT)
Prior art keywords
flow path
valve
base material
sheet material
hole
Prior art date
Application number
PCT/JP2018/016606
Other languages
French (fr)
Japanese (ja)
Inventor
直也 石澤
太郎 上野
遼 小林
哲臣 高崎
Original Assignee
株式会社ニコン
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社ニコン filed Critical 株式会社ニコン
Priority to JP2020515339A priority Critical patent/JP7192859B2/en
Priority to PCT/JP2018/016606 priority patent/WO2019207644A1/en
Publication of WO2019207644A1 publication Critical patent/WO2019207644A1/en

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/08—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N37/00—Details not covered by any other group of this subclass

Definitions

  • the present invention relates to a fluid device, a valve device, and a detection device.
  • ⁇ -TAS is superior to conventional inspection devices in that it can be measured and analyzed with a small amount of sample, can be carried, and can be disposable at low cost. Furthermore, in the case of using an expensive reagent or in the case of testing a small amount of a large number of specimens, it has attracted attention as a highly useful method.
  • Non-Patent Document 1 A device including a flow path and a pump disposed on the flow path as a component of ⁇ -TAS has been reported (Non-Patent Document 1).
  • a plurality of solutions are injected into the channel, and the pump is operated to mix the plurality of solutions in the channel.
  • a first base material and a second base material joined at a joining surface are provided, and at least one of the first base material or the second base material joins both base materials.
  • the first substrate has a through-hole provided at a position facing the flow path, and the opening on the flow path side of the through-hole.
  • An elastic member that adjusts the flow of fluid in the flow path by deformation and a first surface that is opposite to the joint surface of the first base material so as to cover the opening of the through hole and be peelable
  • a fluid device including a valve having a formed sheet material, and a drive unit that is disposed between the sheet material and the elastic member and deforms the elastic member in the flow path direction.
  • the first substrate is positioned at a position facing at least one of the first base material and the second base material joined at the joining surface and opened to the joining surface.
  • a valve device is provided that includes an applying portion that applies a force to close the flow path to the valve portion when the is attached to the first surface.
  • a placement part for placing the fluid device of the first aspect of the present invention, a peeling part for peeling the sheet material, and a target substance in the flow path of the fluid device There are provided a liquid feeding part for feeding a solution containing the liquid and a detection part for detecting a target substance in the solution.
  • 1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment.
  • 1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment.
  • 1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment.
  • Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. The top view which showed typically the fluid device containing the valve apparatus of one Embodiment. Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment.
  • FIGS. 1 to 13 In the drawings used in the following description, in order to make the features easier to understand, the portions that become the features may be shown in an enlarged manner for convenience, and the dimensional ratios of the respective constituent elements may not be the same as the actual ones. I can't.
  • FIG. 1 is a cross-sectional view schematically showing a fluid device 1 including a valve device V.
  • the fluidic device 1 of the present embodiment includes a device that detects and inspects a sample substance that is a detection target included in a specimen sample by an immune reaction, an enzyme reaction, or the like.
  • the sample substance is, for example, a biomolecule such as nucleic acid, DNA, RNA, peptide, protein, extracellular vesicle.
  • the fluid device 1 includes a base material 5 and a valve device V.
  • the substrate 5 has a first substrate 6 and a second substrate 9.
  • the 1st base material 6 and the 2nd base material 9 of this embodiment are comprised from the resin material.
  • the resin material constituting the first base material 6 and the second base material 9 include polypropylene and polycarbonate.
  • the 1st base material 6 is comprised from a transparent material.
  • the material which comprises the 1st base material 6 and the 2nd base material 9 is not limited.
  • first base material 6 and the second base material 9 are described along the horizontal plane, and the first base material 6 is described as being disposed above the second base material 9. However, this only defines the horizontal direction and the vertical direction for convenience of explanation, and does not limit the orientation when the fluidic device 1 according to the present embodiment is used.
  • the first base material 6 and the second base material 9 are plate members extending along the horizontal direction.
  • the 1st base material 6 and the 2nd base material 9 are laminated
  • the second substrate 9 is laminated on the lower side of the first substrate 6.
  • the first base material 6 and the second base material 9 are joined using the lower surface 6a of the first base material 6 and the upper surface 9b of the second base material 9 as joint surfaces.
  • the base material 5 is manufactured by joining and integrating the first base material 6 and the second base material 9 by a joining means such as adhesion using an adhesive, thermal welding, ultrasonic welding, laser welding or the like.
  • the direction in which the first base material 6 and the second base material 9 are stacked is simply referred to as a stacking direction.
  • the stacking direction is the vertical direction.
  • the 1st base material 6 has the flow path 11 and the through-holes 35a and 35b.
  • the flow path 11 is a space formed in a tube shape or a cylindrical shape surrounded by the groove provided on the lower surface 6 a of the first base material 6 and the second base material 9.
  • the channel 11 accommodates a solution for performing the above-described detection / inspection.
  • the through holes 35 a and 35 b are arranged at positions facing both ends of the flow path 11 and penetrate the first base material 6 in the vertical direction.
  • the through holes 35 a and 35 b are, for example, an injection hole and an air hole for a solution stored in the flow path 11.
  • the diameter of the through holes 35a and 35b is preferably 0.1 to 3 mm.
  • the width of the flow path 11 examples include 0.01 to 100 mm, 0.05 to 50 mm, 0.1 to 10 mm, 0.1 to 5 mm, and 0.5 to 3 ⁇ m. Further, as an example, the width of the flow path 11 is 0.01 to 1000 ⁇ m, 0.05 to 1000 ⁇ m, 0.2 to 500 ⁇ m, 1 to 250 ⁇ m, and 10 to 200 ⁇ m. Considering the formability when the first substrate 6 is manufactured by injection molding, the width of the flow path 11 is preferably 0.05 to 5 mm.
  • the depth of the flow path 11 examples include 0.01 to 100 mm, 0.05 to 50 mm, 0.1 to 10 mm, 0.1 to 5 mm, and 0.5 to 3 ⁇ m. Further, as an example, the depth of the flow path 11 is 0.01 to 1000 ⁇ m, 0.05 to 1000 ⁇ m, 0.2 to 500 ⁇ m, 1 to 250 ⁇ m, and 10 to 200 ⁇ m. Considering the moldability when the first substrate 6 is manufactured by injection molding, the depth of the flow path 11 is preferably 0.05 to 3 mm.
  • the valve device V is provided in the second base material 9 and a valve portion Vc held in a valve holding hole (through hole) 34 that penetrates the first base material 6 in the vertical direction at a position facing the flow path 11.
  • a valve holding hole through hole
  • the recess 40 a sheet material 41 that is detachably attached to the upper surface (first surface) 6 b of the first base material 62, and a shaft portion (giving portion, provided on the valve portion Vc) Drive part) 42.
  • the valve portion Vc is provided at a lower end portion of an annular ring portion 44 provided in the valve holding hole 34.
  • the shaft portion 42 is a drive portion that deforms and drives the valve portion Vc.
  • it is a provision part which provides the force which deforms the elastic member which is the valve part Vc, and is a press part which presses an elastic member.
  • the valve part Vc is an elastic member.
  • the valve portion Vc is a valve made of a flexible resin film.
  • the opening on the flow path 11 side of the through hole 34 is closed by an elastic member that is the valve portion Vc.
  • the valve portion Vc is made of an elastic material and adjusts the flow of fluid in the flow path 11 by deformation. Examples of the elastic material that can be used for the valve portion Vc include rubber and elastomer resin.
  • a projecting portion 36 projecting in a hemispherical shape is provided on the lower surface of the valve portion Vc.
  • the depression 40 is disposed immediately below the valve portion Vc on the upper surface 9 b of the second base material 9.
  • the recess 40 is formed in a hemispherical shape.
  • the valve part Vc opens and closes the flow path 11 by deformation.
  • the valve part Vc operates perpendicularly to the flow path direction of the flow path 11 to open and close the flow path 11.
  • the valve portion Vc is elastically deformed downward, and the projecting portion 36 comes into contact with the recess 40 to close the flow path 11. Further, the valve portion Vc opens the flow path 11 as shown in FIG. 1 by the protrusion 36 being separated from the recess 40 by the elastic restoring force.
  • the shaft portion 42 has a pillar shape substantially perpendicular to the flow path 11.
  • the shaft part 42 is formed in such a length that the upper end protrudes from the upper surface 6 b of the first base material 6. Further, the length of the shaft portion 42 is such that the upper end surface is substantially flush with the upper surface 6b of the first base material 62 or the upper surface 6b when the valve portion V is elastically deformed and the protruding portion 36 comes into contact with the recess 40. It is a length protruding upward. That is, the length of the shaft portion 42 in the penetrating direction of the valve holding hole 34 projects from the upper surface 6b before the sheet material 41 is attached to the upper surface 6b, and after the sheet material 41 is attached to the upper surface 6b. Is a length to close the flow path 11 by deforming.
  • the height from the upper surface of the valve portion Vc to the lower surface of the sheet material 41 when the lower surface of the valve portion Vc comes into contact with the bottom surface of the flow path 11 and the height of the shaft portion 42 that is an application portion (drive portion) are: Except for the amount of deformation of the valve portion Vc due to elastic deformation, it is substantially the same. In other words, the difference obtained by subtracting the thickness of the valve portion Vc from the sum of the height from the bottom surface of the flow channel 11 to the ceiling surface of the flow channel 11 and the thickness of the first base member 6 and the height of the shaft portion 42 are elastic. Except for the amount of deformation of the valve portion Vc due to deformation, it is substantially the same.
  • the valve portion Vc, the protruding portion 36, and the shaft portion 42 are formed bodies that are integrally formed of the above-described flexible elastic material.
  • the sheet material 41 is used for stable storage of the solution stored in the flow path 11 and prevention of moisture absorption of the solution during storage and transportation of the fluid device 1 before performing the detection and inspection described above.
  • the sheet material 41 is formed of a thin film material such as a film.
  • the sheet material 41 is detachably attached to the upper surface 6b of the first base material 6.
  • the sheet material 41 may have an adhesive layer on the contact surface side with the upper surface 6 b of the first base material 6. Further, the sheet material 41 may be detachably attached to the upper surface 6b of the first base material 6 via an adhesive.
  • the sheet material 41 in the present embodiment has a size that covers the valve holding hole 34 and the openings of the through holes 35a and 35b.
  • the first base member 6, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are integrally formed molded bodies.
  • the first base material 6, the valve portion Vc, the projecting portion 36, and the shaft portion 42 are formed using the mold in which the first base material 6 is accommodated after the first base material 6 is molded.
  • the portion 36 and the shaft portion 42 are molded by two-color molding.
  • FIGS. 3 and 4 are schematic configuration diagrams showing a first embodiment of a system having a sticking device 60 for sticking the sheet material 41 to the fluid device 1 described above.
  • the system includes a roller device 61 as the sticking device 60.
  • the roller device 61 rolls on the upper surface 6 b of the first base material 6 via the sheet material 41.
  • the roller device 61 moves from the end portion on one side (right side in FIG. 3) to the other side ( It rolls on the upper surface 6b through the sheet material 41 until the left side in FIG. By being pressed by the roller device 61, the sheet material 41 is bonded to the upper surface 6b.
  • the shaft portion 42 is pushed downward. That is, by sticking the sheet material 41, the shaft portion 42 is pushed in a direction substantially perpendicular to the direction in which the fluid flows in the flow path.
  • the valve portion Vc is elastically deformed in a direction substantially perpendicular to the direction in which the fluid flows in the flow path, and the flow path 11 is closed by the protrusion 36 coming into contact with the recess 40.
  • the sheet material 41 is peeled from the upper surface 6b.
  • the sheet material 41 is pierced or broken in the through hole 35.
  • the force applied to the shaft portion 42 by the sheet material 41 is removed.
  • the valve portion Vc is separated from the recess 40 by the elastic restoring force, as shown in FIG. 1, and the flow path 11 is opened.
  • the valve portion Vc functions as an initial close valve (normally close valve). That is, when the sheet material 41 is attached to the upper surface 6b, the valve portion Vc is in a closed state in which the flow of the fluid in the flow path 11 is blocked, so that the flow path is stored and transported before detection and inspection. It can suppress that the solution accommodated in 11 leaks, or the solutions before mixing are mixed.
  • the sheet material 41 is peeled from the upper surface 6b, whereby the valve portion Vc is deformed into an open state in which the fluid in the flow path 11 flows, and the blockage of the flow path 11 is released. It is possible to perform detection and inspection using the fluid device 1 smoothly and stably after transportation.
  • the roller device 61 rolls on the upper surface 6b via the sheet material 41, whereby the sheet material 41 can be easily attached to the upper surface 6b. In particular, when the sheet material 41 covers all the openings of the through holes 34, 35a, and 35b, the sheet material 41 can be easily attached.
  • the sheet material 41 does not cover the entire upper surface 6b but may partially cover the openings on the upper surface 6b side of the through holes 34, 35a, and 35b. Further, the sheet materials 41 covering the openings of the through holes 34, 35a, and 35b may be different from each other. In this case, a small amount of sheet material is sufficient, and the through holes 34, 35a, and 35b can be released stepwise.
  • FIGS. 1 to 4 the same components as those of the first embodiment shown in FIGS. 1 to 4 are denoted by the same reference numerals, and the description thereof is omitted. Since the difference between the second embodiment and the first embodiment is the configuration of the sticking device 60, the sticking device 60 will be described below.
  • the fluid device 1 is packaged using the bag body 62 during storage and transportation before detection and inspection.
  • a region facing the upper surface 6 b of the bag body 62 is the sheet material 41.
  • a suction device 63 is provided as the sticking device 60. The suction device 63 sucks the inside of the bag body 62 through the opening of the bag body 62 under negative pressure.
  • the suction device 63 sucks the inside of the bag body 62 with a negative pressure, so that the pressure inside the bag body 62 becomes lower than the outside pressure.
  • the bag body 62 is pressed and attached to the outer surface of the base material 5 including the upper surface 6 b by atmospheric pressure.
  • the shaft portion 42 is pushed downward as described above.
  • the valve portion Vc is in a state in which the flow path 11 is closed by the protrusion 36 coming into contact with the recess 40.
  • the sealing portion 64 is provided at the mouth portion of the bag body 62 by welding or the like, whereby the blockage of the flow path 11 by the valve portion Vc is maintained. Further, when performing detection / inspection using the fluid device 1, the valve portion Vc has an elastic restoring force as shown in FIG. Thus, the channel 11 is released from the recess 40 and opened.
  • the fluid device 1 is packaged by the bag body 62 before detection and inspection. Contamination of the fluid device 1 with dust or the like before detection / inspection can be suppressed.
  • FIGS. 1 and 2 the same components as those of the first embodiment shown in FIGS. 1 and 2 are denoted by the same reference numerals, and the description thereof is omitted.
  • FIG. 7 is a schematic cross-sectional view of a detection apparatus 70 that performs detection using the fluidic device 1 according to the second embodiment.
  • FIG. 8 is a plan view schematically showing the fluidic device 1. In addition, in FIG. 8, it illustrates in the state which permeate
  • the detection device 70 includes a placement unit 71 for placing the fluid device 1, a peeling unit 72 for peeling the sheet material 41, and a liquid feeding unit 73 for feeding a solution containing the target substance to the flow path 11 of the fluid device 1. And a detection unit 74 that detects a target substance in the solution.
  • the fluid device 1 includes a base material 5.
  • the base material 5 has three board
  • the third base material 8 is formed of the same resin material as the first base material 6 and the second base material 9, and is disposed below the second base material 9.
  • the upper surface 8 b of the third base material 8 is joined to the lower surface 9 a of the second base material 9.
  • the base material 5 includes an injection hole 32, a reservoir 29, a flow path 11, a waste liquid tank 7, an air hole 35, a supply hole 39, a metering valve Vq, an introduction valve Vi, a discharge valve Vo, and the valves described above.
  • a device V is provided.
  • the injection hole 32 penetrates the first base material 6 and the second base material 9.
  • the injection hole 32 is connected to a reservoir 29 located at the boundary between the second base material 9 and the third base material 8.
  • the injection hole 32 connects the reservoir 29 to the outside.
  • the solution is filled into the reservoir 29 through the injection hole 32.
  • One injection hole 32 is provided for one reservoir 29. In FIG. 8, the injection hole 32 is not shown.
  • the reservoir 29 has a flow channel shape at least in the connection portion with the flow channel 11. For example, it is a space formed in a tubular shape or a cylindrical shape provided on the lower surface 9 a of the second base material 9.
  • the substrate 5 of the present embodiment is provided with a plurality of reservoirs 29.
  • the reservoir 29 stores a solution.
  • the plurality of reservoirs 29 contain solutions independently of each other.
  • the plurality of reservoirs 29 contain, for example, a plurality of reagents.
  • Each of the plurality of reservoirs 29 is provided with the above-described initial close valve.
  • the reservoir 29 supplies the stored solution to the flow path 11.
  • the reservoir 29 of this embodiment is a flow path type reservoir as an example. One end of the reservoir 29 in the length direction is connected to the injection hole 32. Further, the supply hole 39 is connected to the other end of the reservoir 29 in the length direction.
  • the reservoir 29 is provided on the second base material 9 and the opening is covered with the third base material 8 has been described.
  • the reservoir 29 may be configured to be provided in the third base material 8 and cover the opening with the second base material 9.
  • the flow path 11 is a space formed in a tube shape or a cylindrical shape provided on the upper surface 9 b of the second base material 9.
  • the solution is supplied to the flow path 11 from the reservoir 29 through the supply hole 39.
  • the solution flows in the flow path 11.
  • the supply hole 39 is provided in the second base material 9.
  • the supply hole 39 penetrates the second base material 9 in the plate thickness direction.
  • the supply hole 39 connects the reservoir 29 and the flow path 11.
  • the solution stored in the reservoir 29 is supplied to the flow path 11 through the supply hole 39. That is, the reservoir 29 is connected to the flow path 11 through the supply hole 39.
  • the waste liquid tank 7 is provided on the base material 5 in order to discard the solution in the flow path 11.
  • the waste liquid tank 7 is connected to the flow path 11.
  • the waste liquid tank 7 is disposed in the inner region of the circulation channel 10. Thereby, size reduction of the fluid device 1 can be achieved.
  • the waste liquid tank 7 is constituted by a concave stitch provided on the upper surface 9 b side of the second base material 9.
  • the air hole 35 is provided in the first base material 6.
  • the air hole 35 is located immediately above the waste liquid tank 7.
  • the air hole 35 connects the waste liquid tank 7 to the outside. That is, the waste liquid tank 7 is opened to the outside through the air hole 35.
  • the introduction valve Vi is disposed at a position facing the flow path 11 between the supply hole 39 and the waste liquid tank 7.
  • the introduction valve Vi has a valve portion Vd held in a valve holding hole 34 i that penetrates the first base material 6 in the vertical direction at a position facing the flow path 11.
  • the valve portion Vd is formed of an elastic material such as rubber or elastomer resin.
  • a projecting portion 36i projecting in a hemispherical shape is provided on the lower surface of the valve portion Vd.
  • a recess 40i is provided in the second base material 9 facing the valve portion Vd.
  • the valve portion Vd is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34i, and adjusts the flow of the solution in the flow path 11.
  • the valve portion Vd is supplied with driving fluid into the valve holding hole 34i and elastically deforms downward, and the projecting portion 36i contacts the recess 40i to close the flow path 11.
  • the valve portion Vd opens the flow path 11 by separating the protruding portion 36i from the recess 40i by an elastic restoring force.
  • the discharge valve Vo is disposed at a position facing the flow path 11 between the introduction valve Vi and the waste liquid tank 7.
  • the discharge valve Vo has a valve portion Vf held in a valve holding hole 34o penetrating the first base material 6 in the vertical direction at a position facing the flow path 11.
  • the valve portion Vf is formed of an elastic material such as rubber or elastomer resin.
  • a projecting portion 36o projecting in a hemispherical shape is provided on the lower surface of the valve portion Vf.
  • the second base material 9 facing the valve portion Vf is provided with a recess 40o.
  • the valve portion Vf is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34o and adjusts the flow of the solution in the flow path 11.
  • a driving fluid for example, air
  • the valve portion Vf is supplied with driving fluid into the valve holding hole 34o and elastically deforms downward, and the projecting portion 36o contacts the recess 40o to close the flow path 11.
  • the valve portion Vf opens the flow path 11 by separating the protruding portion 36i from the recess 40o by an elastic restoring force.
  • the metering valve Vq has a valve portion Ve held by a valve holding hole 34q that penetrates the first base material 6 in a vertical direction at a position facing the flow path 11.
  • the valve portion Ve is formed of an elastic material such as rubber or elastomer resin.
  • a projecting portion 36q projecting in a hemispherical shape is provided on the lower surface of the valve portion Ve.
  • the second base material 9 facing the valve portion Ve is provided with a recess 40q.
  • the valve portion Ve is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34q, and adjusts the flow of the solution in the flow path 11.
  • a driving fluid for example, air
  • the valve portion Ve is supplied with driving fluid into the valve holding hole 34q and elastically deforms downward, and the projecting portion 36q contacts the recess 40q to close the flow path 11.
  • the valve portion Ve opens the flow path 11 by separating the protruding portion 36q from the recess 40q by an elastic restoring force.
  • the valve device V described above is disposed at a position facing the flow path 11 between the supply hole 39 and the introduction valve Vi.
  • the first base material 6, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are formed integrally.
  • the first base material 6, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36 and the shaft portion 42 are accommodated in the first base material 6 after the first base material 6 is formed, for example.
  • the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are molded by the two-color molding using the molded mold.
  • one of the first base 6 manufactured in advance, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36 and the shaft portion 42 is mounted on a mold for molding the other. It is also possible to mold by insert molding.
  • the introduction valve Vi, the discharge valve Vo, and the metering valve Vq are initial open valves (second valves).
  • the initial close valve and the initial open valve described above are arranged in series in the flow path 11.
  • the sheet material 41 is attached to the upper surface 6b in a state where the solution is stored in the reservoir 29 before detection and inspection.
  • a high-viscosity reagent is accommodated in the flow path 11 between the introduction valve Vi and the discharge valve Vo as an example.
  • the injection hole 32 and the air hole 35 are closed by the sheet material 41.
  • the flow path 11 and the reservoir 29 are not released to the atmosphere. Therefore, the solution stored in the reservoir 29 is held in the reservoir 29 with the flow suppressed, and the reagent stored in the flow path 11 is held in the flow path 11 with the flow suppressed. Further, in the valve portion Vc, the projecting portion 36 abuts the recess 40, the flow path 11 is closed, and the reservoir 29 and the flow path 11 are separated. Therefore, even when a large acceleration is applied to the fluid device 1, It can suppress mixing with a reagent. Furthermore, in the fluid device 1 described above, since the air flow in the reservoir 29 and the flow path 11 is also suppressed, it is possible to suppress the evaporation of the solution and the reagent.
  • the channel 11 is not open to the atmosphere, the flow of the solution, reagent, and air in the reservoir 29 and the channel 11 is suppressed.
  • the flow path 11 includes a circulation flow path 10, a plurality (three in the example of FIG. 8) introduction flow paths 12, and a plurality (three in the example of FIG. 8) discharge flow paths 13. And including.
  • a solution is introduced into the channel 11 from a reservoir 29 (see FIG. 7).
  • the circulation channel 10 is configured in a loop shape when viewed from the stacking direction.
  • a pump P is disposed in the path of the circulation channel 10.
  • the pump P is composed of three element pumps Pe arranged side by side in the flow path.
  • the element pump Pe is a so-called valve pump.
  • the pump P can convey the liquid in the circulation channel by sequentially opening and closing the three element pumps Pe.
  • the number of element pumps Pe constituting the pump P may be four or more.
  • a plurality of (three in the example of FIG. 8) metering valves Vq are provided in the path of the circulation channel 10.
  • the plurality of metering valves Vq partitions the circulation channel 10 into a plurality of metering sections 18.
  • the plurality of metering valves Vq are arranged so that each metering section 18 has a predetermined volume.
  • a meandering portion 18 a is provided in one of the three quantitative sections 18.
  • the meandering portion 18a is a channel formed by meandering left and right.
  • the meandering portion 18a is provided in order to make one quantitative section 18 have a desired capacity.
  • Quantitative sections 18 each extend in a flow path shape.
  • the plurality of fixed-quantity sections 18 each have a flow path-shaped transfer flow path 80, an inflow portion 81 located at one end of the transfer flow path 80, and a merge portion provided at the other end of the transfer flow path 80. 85. Therefore, in each quantitative section 18, the transfer flow path 80 is located between the inflow portion 81 and the merging portion 85.
  • the inflow portion 81 of one quantitative section 18 is connected to the merging section 85 of another quantitative section 18 via a quantitative valve Vq.
  • the introduction flow path 12 is connected to the inflow portion 81 via the introduction valve Vi.
  • the merging section 85 of one quantitative section 18 is connected to an inflow section 81 of another quantitative section 18 via a quantitative valve Vq.
  • the discharge flow path 13 is connected to the junction 85 via a discharge valve Vo.
  • the introduction flow path 12 is a flow path for introducing the solution into the quantitative section 18 of the circulation flow path 10.
  • the introduction channel 12 is provided for each quantitative section 18 of the circulation channel 10.
  • the introduction channel 12 is connected to the supply hole 39 on one end side.
  • the introduction flow path 12 is connected to the inflow portion 81 of the circulation flow path 10 on the other end side.
  • the discharge channel 13 is a channel for discharging the solution in the quantitative section 18 of the circulation channel 10 to the waste liquid tank 7.
  • the discharge channel 13 is provided for each quantitative section 18 of the circulation channel 10.
  • the discharge channel 13 is connected to the waste liquid tank 7 at one end side. Further, the discharge flow channel 13 is connected to the merging portion 85 of the circulation flow channel 10 on the other end side.
  • the fluid device 1 In placing the fluid device 1 on the placement part 71, the fluid device 1 is positioned so that the lower surface 8 a of the third base material 8 in the fluid device 1 is in contact with the upper surface of the placement part 71.
  • the peeling portion 72 moves relative to the upper surface 6 b of the first base material 6 with one end of the sheet material 41 sucked, for example, under a negative pressure.
  • the material 41 is peeled from the upper surface 6b.
  • the placement step for example, when the lower surface 8a of the third base material 8 in the fluid device 1 is bonded to the upper surface of the placement portion 71 or the fluid device 1 is fixed to the placement portion 71. Is easy to peel.
  • the solution S stored in the reservoir 29 is sent to the flow path 11 by the liquid feeding unit 73 after the sheet material 41 is peeled from the fluid device 1.
  • the opening and closing of the valves Vq, Vi, and Vo which will be described later, is performed by switching the supply and stop of the supply of the driving fluid to the valve holding holes 34q, 34i, and 34o. Omitted.
  • the reservoir 29 is preliminarily held and / or filled with the solution S.
  • the sheet material 41 is peeled from the upper surface 6 b of the first base 6, whereby the protrusion 36 in the valve device V is separated from the recess 40 and the flow path 11 is formed. Opened.
  • the solution and the reagent are in a state in which evaporation and mixing are suppressed.
  • the solution S in the reservoir 29 is moved to the flow path 11. More specifically, the solution S is sequentially introduced from the reservoir 29 into each quantitative section 18 of the circulation channel 10.
  • the procedure for introducing the solution S into one quantitative section 18 will be described, but the solution S is also introduced by performing the same procedure for the other quantitative sections 18.
  • valves Vq, Vi, Vo when introducing the solution S into the quantitative section 18 will be described with reference to FIG.
  • the pair of metering valves Vq located on both sides in the length direction of the metering section 18 for introducing the solution S are closed.
  • the discharge valve Vo of the discharge flow path 13 connected to the corresponding quantitative section 18 is opened, and the discharge valves Vo of the other discharge flow paths 13 are closed.
  • the introduction valve Vi of the introduction flow path 12 connected to the corresponding quantitative section 18 is opened.
  • the detection unit 74 shown in FIG. 7 detects the solution mixed in the flow path 11.
  • the target substance is, for example, a sample substance included in a specimen sample.
  • the sample substance is, for example, a biomolecule such as a nucleic acid, DNA, RNA, peptide, protein, extracellular vesicle or particle.
  • substances mixed with the target substance in the solution include a labeling substance (detection auxiliary substance) that binds to the sample substance and assists the detection of the sample substance, and a substance that captures and collects the sample substance in the solution. .
  • the detection unit 74 for example, a configuration in which the target substance is imaged and detected via the first base material 6 by an imaging element, or a configuration in which the target substance is detected by measuring the magnetic force via the first base material 6 Can be taken.
  • Insert molding may be performed in which molding is performed in a state in which the metered valve Vq, the introduction valve Vi, the discharge valve Vo, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are stored.
  • the shaft portion 42 provided in the valve portion Vc is exemplified as the applying portion that applies the force for closing the flow path 11 to the valve portion.
  • the present invention is not limited to this configuration.
  • the granular member 42A loaded in the valve holding hole 34 between the seat material 41 and the valve portion Vc may be used.
  • the granular member 42A may have a cylindrical shape, a cubic shape or a rectangular parallelepiped shape in addition to the spherical shape shown in FIG.
  • the granular member 42A is preferably spherical from the viewpoint of maintaining the distance between the seat member 41 and the valve portion Vc at a predetermined value without being affected by the posture after being charged.
  • the granular member 42A attached to the sheet material 41 can be easily and collectively removed when the sheet material 41 having the adhesive layer on the lower surface is peeled off from the upper surface 6b. It can be removed.
  • the application portion is provided to protrude downward from the lower surface of the sheet material 41 as shown in FIG.
  • the structure used as the axial part 42B may be sufficient. Even in this configuration, since the application portion does not protrude from the upper surface 6b after the sheet material 41 is peeled off, for example, a recess for preventing interference with the application portion needs to be formed in the member for injecting the solution into the through hole 35a. Disappear.
  • valve portion Vc is a cylindrical shape attached to the inner peripheral surface of the valve holding hole 34
  • the present invention is not limited to this configuration.
  • a fitting convex portion 34 a that protrudes annularly radially inward is provided in the middle in the vertical direction on the inner peripheral surface of the valve holding hole 34, and the fitting convex portion 34 a is provided on the valve portion Vc.
  • the structure which provides the fitting recessed part 43 to fit may be sufficient. Since the valve portion Vc having this configuration is engaged with the fitting convex portion 34a from both sides in the vertical direction, the valve portion Vc is prevented from being detached from the first base material 6 when the sheet material 41 is attached. it can.
  • FIG. 12 is a plan view of the second base material 9 in which the flow path 11 is formed.
  • a recess 40 is formed in the upper surface 9b of the second base material 9 at a position facing the protruding portion 36 of the valve portion Vc, as shown in FIG.
  • the recess 40 is disposed in the middle of the flow path 11.
  • 13 is a cross-sectional view taken along line AA in FIG. FIG.
  • the bottom surface of the channel 11 is inclined upward from the position of the ridge line of the recess 40 in the upper surface 9b.
  • valve portion Vc is elastically deformed downward, and the projecting portion 36 abuts on the recess 40 to close the flow path 11.
  • the flow path 11 can be more reliably closed.
  • the depth of the flow path 11 is 0.05 mm or more and 3 mm or less (for example, 0.3 mm).
  • Examples of the diameter D of the recess around the shaft portion 42 include 1 mm or more and 5 mm or less (for example, 2.2 mm).
  • Examples of the diameter of the shaft portion 42 include 0.5 mm or more and 4 mm or less (for example, 1.0 mm).
  • Examples of the protruding amount of the shaft portion 42 from the upper surface 6b include 0.05 mm or more and 3 mm or less (for example, 0.4 mm).
  • the gap amount t between the lower end of the protrusion 36 and the lower end of the recess 40 when the valve portion Vc opens the flow path 11 is 0.05 mm or more and 1.0 mm or less (for example, 0.1 mm).
  • Examples of the diameter of the circle formed by the ridge line of the recess 40 in the upper surface 9b include 0.5 mm or more and 5.0 mm or less (for example, 2.0 mm).
  • Examples of the radius RA of the spherical surface of the recess 40 include 1.0 mm or more and 5.0 mm or less (for example, 1.45 mm).
  • Examples of the radius RB of the spherical surface of the protrusion 36 include 1.0 mm or more and 5.0 mm or less (for example, 1.82 mm).
  • bulb Vc has the protrusion part 36
  • valve part Vc illustrated the structure which opens and closes the flow path 11 by sticking and peeling of the sheet
  • the flow of the flow path 11 is controlled by supplying and stopping the supply of the driving fluid to the valve holding hole 34 (solution flow adjustment ) May be switched.
  • the upper end of the provision part was illustrated in the same position as the upper surface 6b, However, It is not limited to this structure, Affixed The upper end of the applying portion may protrude from the upper surface 6b as long as the sheet material 41 does not peel off.
  • seat material 41 has an adhesive layer was illustrated, it is not limited to this structure, For example, a sheet
  • the structure which affixes material on the upper surface 6b may be sufficient.

Abstract

The purpose of the present invention is to provide a fluid device capable of stable detection and inspection after storage and transport. The present invention comprises a first substrate and second substrate joined at a joining surface. At least one from among the first substrate and second substrate has, at the joining surface, a groove that forms a flow path as a result of the joining of the substrates. The first substrate comprises a valve having: a through hole provided at a position opposing the flow path; an elastic member that blocks the flow-path side opening of the through hole and adjusts the flow of the fluid through the flow path by deforming; a sheet member that is peelably affixed to a first surface, which is on the reverse side of the first substrate from the joining surface, so as to cover an opening of the through hole; and a drive unit that is disposed between the sheet member and elastic member and deforms the elastic member in the flow path direction.

Description

流体デバイス、バルブ装置及び検出装置Fluid device, valve device and detection device
　本発明は、流体デバイス、バルブ装置及び検出装置に関するものである。 The present invention relates to a fluid device, a valve device, and a detection device.
　近年、体外診断分野における試験の高速化、高効率化、および集積化、又は、検査機器の超小型化を目指したμ－ＴＡＳ（Micro-Total Analysis Systems）の開発などが注目を浴びており、世界的に活発な研究が進められている。 In recent years, the development of μ-TAS (Micro-Total Analysis Systems) aimed at speeding up, increasing efficiency and integration of tests in the field of in-vitro diagnosis, or ultra-miniaturization of test equipment has attracted attention. Active research is ongoing worldwide.
　μ－ＴＡＳは、少量の試料で測定、分析が可能なこと、持ち運びが可能となること、低コストで使い捨て可能なこと等、従来の検査機器に比べて優れている。
　更に、高価な試薬を使用する場合や少量多検体を検査する場合において、有用性が高い方法として注目されている。 μ-TAS is superior to conventional inspection devices in that it can be measured and analyzed with a small amount of sample, can be carried, and can be disposable at low cost.
Furthermore, in the case of using an expensive reagent or in the case of testing a small amount of a large number of specimens, it has attracted attention as a highly useful method.
　μ－ＴＡＳの構成要素として、流路と、該流路上に配置されるポンプとを備えたデバイスが報告されている（非特許文献１）。このようなデバイスでは、該流路へ複数の溶液を注入し、ポンプを作動させることで、複数の溶液を流路内で混合する。 A device including a flow path and a pump disposed on the flow path as a component of μ-TAS has been reported (Non-Patent Document 1). In such a device, a plurality of solutions are injected into the channel, and the pump is operated to mix the plurality of solutions in the channel.
　本発明の第１の態様に従えば、接合面で接合された第１基材及び第２基材を備え、前記第１基材又は前記第２基材の少なくとも一方は、両基材を接合することにより流路を形成する溝を接合面に有し、前記第１基材は、前記流路と対向する位置に設けられる貫通孔と、前記貫通孔の前記流路側の開口部を塞ぎ、変形により前記流路中の流体の流れを調整する弾性部材と、前記第１基材の前記接合面とは逆側の第１面に、前記貫通孔の開口部を覆って剥離可能に貼り付けられたシート材と、前記シート材と前記弾性部材との間に配置され、前記弾性部材を前記流路方向に変形させる駆動部と、を有するバルブを備える、流体デバイスが提供される。 According to the first aspect of the present invention, a first base material and a second base material joined at a joining surface are provided, and at least one of the first base material or the second base material joins both base materials. The first substrate has a through-hole provided at a position facing the flow path, and the opening on the flow path side of the through-hole. An elastic member that adjusts the flow of fluid in the flow path by deformation and a first surface that is opposite to the joint surface of the first base material so as to cover the opening of the through hole and be peelable There is provided a fluid device including a valve having a formed sheet material, and a drive unit that is disposed between the sheet material and the elastic member and deforms the elastic member in the flow path direction.
　本発明の第２の態様に従えば、接合面で接合された第１基材及び第２基材の少なくとも一方に前記接合面に開口して形成された流路と対向する位置で前記第１基材を貫通する貫通孔と、前記貫通孔の前記流路側に当該流路と対向して設けられ、弾性変形により前記流路中の流体の流れを調整するバルブ部と、前記第１基材の前記接合面と逆側の第１面に剥離可能に貼り付けられ前記貫通孔を閉塞可能なシート材と、前記貫通孔の前記シート材と前記バルブ部との間に配置され、前記シート材が前記第１面に貼り付けられたときに、前記バルブ部に前記流路を閉じる力を付与する付与部と、を備えるバルブ装置が提供される。 According to the second aspect of the present invention, the first substrate is positioned at a position facing at least one of the first base material and the second base material joined at the joining surface and opened to the joining surface. A through-hole penetrating the base material, a valve portion provided on the channel side of the through-hole facing the channel, and adjusting the flow of fluid in the channel by elastic deformation; and the first base material A sheet material that is detachably attached to the first surface opposite to the joint surface, and is disposed between the sheet material and the valve portion of the through hole, and the sheet material A valve device is provided that includes an applying portion that applies a force to close the flow path to the valve portion when the is attached to the first surface.
　本発明の第３の態様に従えば、本発明の第１の態様の流体デバイスを載置する載置部と、前記シート材を剥離する剥離部と、前記流体デバイスの流路に対象物質を含む溶液を送液する送液部と、前記溶液中の対象物質を検出する検出部が提供される。 According to the third aspect of the present invention, a placement part for placing the fluid device of the first aspect of the present invention, a peeling part for peeling the sheet material, and a target substance in the flow path of the fluid device. There are provided a liquid feeding part for feeding a solution containing the liquid and a detection part for detecting a target substance in the solution.
一実施形態のバルブ装置を含む流体デバイスを模式的に示した断面図。Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. 一実施形態のバルブ装置を含む流体デバイスを模式的に示した断面図。Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. 一実施形態の流体デバイスを含むシステムを模式的に示した断面図。1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment. 一実施形態の流体デバイスを含むシステムを模式的に示した断面図。1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment. 一実施形態の流体デバイスを含むシステムを模式的に示した断面図。1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment. 一実施形態の流体デバイスを含むシステムを模式的に示した断面図。1 is a cross-sectional view schematically showing a system including a fluidic device according to an embodiment. 一実施形態の流体デバイスを模式的に示す断面図である。It is sectional drawing which shows typically the fluid device of one Embodiment. 一実施形態の流体デバイスを模式的に示す平面図である。It is a top view showing typically the fluidic device of one embodiment. 一実施形態のバルブ装置を含む流体デバイスを模式的に示した断面図。Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. 一実施形態のバルブ装置を含む流体デバイスを模式的に示した断面図。Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. 一実施形態のバルブ装置を含む流体デバイスを模式的に示した断面図。Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment. 一実施形態のバルブ装置を含む流体デバイスを模式的に示した平面図。The top view which showed typically the fluid device containing the valve apparatus of one Embodiment. 一実施形態のバルブ装置を含む流体デバイスを模式的に示した断面図。Sectional drawing which showed typically the fluidic device containing the valve apparatus of one Embodiment.
　以下、本発明の流体デバイス、バルブ装置及び検出装置の実施の形態を、図１ないし図１３を参照して説明する。なお、以下の説明で用いる図面は、特徴をわかりやすくするために、便宜上特徴となる部分を拡大して示している場合があり、各構成要素の寸法比率などが実際と同じであるとは限られない。 Hereinafter, embodiments of the fluid device, the valve device, and the detection device of the present invention will be described with reference to FIGS. 1 to 13. In the drawings used in the following description, in order to make the features easier to understand, the portions that become the features may be shown in an enlarged manner for convenience, and the dimensional ratios of the respective constituent elements may not be the same as the actual ones. I can't.
［流体デバイス及びバルブ装置の基本原理］
　図１は、バルブ装置Ｖを含む流体デバイス１を模式的に示した断面図である。
　本実施形態の流体デバイス１は、検体試料に含まれる検出対象である試料物質を免疫反応および酵素反応などにより検出・検査するデバイスを含む。試料物質は、例えば、核酸、ＤＮＡ、ＲＮＡ、ペプチド、タンパク質、細胞外小胞体などの生体分子である。 [Basic principles of fluid devices and valve devices]
FIG. 1 is a cross-sectional view schematically showing a fluid device 1 including a valve device V.
The fluidic device 1 of the present embodiment includes a device that detects and inspects a sample substance that is a detection target included in a specimen sample by an immune reaction, an enzyme reaction, or the like. The sample substance is, for example, a biomolecule such as nucleic acid, DNA, RNA, peptide, protein, extracellular vesicle.
　図１に示すように、流体デバイス１は、基材５とバルブ装置Ｖとを備える。
　基材５は、第１基材６および第２基材９を有する。本実施形態の第１基材６および第２基材９は、樹脂材料から構成される。第１基材６および第２基材９を構成する樹脂材料としては、ポリプロピレン、ポリカーボネイト等が例示される。また、本実施形態において、第１基材６は、透明な材料から構成される。なお、第１基材６および第２基材９を構成する材料は、限定されない。 As shown in FIG. 1, the fluid device 1 includes a base material 5 and a valve device V.
The substrate 5 has a first substrate 6 and a second substrate 9. The 1st base material 6 and the 2nd base material 9 of this embodiment are comprised from the resin material. Examples of the resin material constituting the first base material 6 and the second base material 9 include polypropylene and polycarbonate. Moreover, in this embodiment, the 1st base material 6 is comprised from a transparent material. In addition, the material which comprises the 1st base material 6 and the 2nd base material 9 is not limited.
　以下の説明においては、第１基材６および第２基材９は水平面に沿って配置され、第１基材６は第２基材９の上側に配置されるものとして説明する。ただし、これは、説明の便宜のために水平方向および上下方向を定義したに過ぎず、本実施形態に係る流体デバイス１の使用時の向きを限定しない。 In the following description, the first base material 6 and the second base material 9 are described along the horizontal plane, and the first base material 6 is described as being disposed above the second base material 9. However, this only defines the horizontal direction and the vertical direction for convenience of explanation, and does not limit the orientation when the fluidic device 1 according to the present embodiment is used.
　第１基材６および第２基材９は、水平方向に沿って延びる板材である。第１基材６および第２基材９は、上下方向に沿ってこの順で積層されている。第２基材９は、第１基材６の下側に積層される。第１基材６および第２基材９は、第１基材６の下面６ａと第２基材９の上面９ｂを接合面として接合されている。基材５は、第１基材６および第２基材９を接着材による接着、熱溶着、超音波溶着、レーザー溶着等の接合手段により接合して一体化することにより製造される。
　なお、以下の説明において、第１基材６および第２基材９を積層させる方向を単に積層方向と呼ぶ。本実施形態において、積層方向は、上下方向である。 The first base material 6 and the second base material 9 are plate members extending along the horizontal direction. The 1st base material 6 and the 2nd base material 9 are laminated | stacked in this order along the up-down direction. The second substrate 9 is laminated on the lower side of the first substrate 6. The first base material 6 and the second base material 9 are joined using the lower surface 6a of the first base material 6 and the upper surface 9b of the second base material 9 as joint surfaces. The base material 5 is manufactured by joining and integrating the first base material 6 and the second base material 9 by a joining means such as adhesion using an adhesive, thermal welding, ultrasonic welding, laser welding or the like.
In the following description, the direction in which the first base material 6 and the second base material 9 are stacked is simply referred to as a stacking direction. In the present embodiment, the stacking direction is the vertical direction.
　図１に示すように、第１基材６は、流路１１と、貫通孔３５ａ、３５ｂとを有している。流路１１は、第１基材６の下面６ａに設けられた溝部と第２基材９とによって囲まれたチューブ状、あるいは筒状に形成された空間である。流路１１は、上述した検出・検査を行うための溶液を収容する。貫通孔３５ａ、３５ｂは、流路１１の両端部と対向する位置に配置され、第１基材６を上下方向に貫通する。貫通孔３５ａ、３５ｂは、一例として、流路１１に収容する溶液の注入孔、空気孔である。貫通孔３５ａ、３５ｂの孔径としては、一例として、直径０．１～３ｍｍが好ましい。 As shown in FIG. 1, the 1st base material 6 has the flow path 11 and the through- holes 35a and 35b. The flow path 11 is a space formed in a tube shape or a cylindrical shape surrounded by the groove provided on the lower surface 6 a of the first base material 6 and the second base material 9. The channel 11 accommodates a solution for performing the above-described detection / inspection. The through holes 35 a and 35 b are arranged at positions facing both ends of the flow path 11 and penetrate the first base material 6 in the vertical direction. The through holes 35 a and 35 b are, for example, an injection hole and an air hole for a solution stored in the flow path 11. As an example, the diameter of the through holes 35a and 35b is preferably 0.1 to 3 mm.
　流路１１の幅は一例として、０．０１～１００ｍｍ、０．０５～５０ｍｍ、０．１～１０ｍｍ、０．１～５ｍｍ、０．５～３μｍが挙げられる。また、さらに一例として、流路１１の幅は、０．０１～１０００μｍ、０．０５～１０００μｍ、０．２～５００μｍ、１～２５０μｍ、１０～２００μｍが挙げられる。第１基材６を射出成形で製作する場合の成形性を考慮すると、流路１１の幅は０．０５～５ｍｍが好ましい。 Examples of the width of the flow path 11 include 0.01 to 100 mm, 0.05 to 50 mm, 0.1 to 10 mm, 0.1 to 5 mm, and 0.5 to 3 μm. Further, as an example, the width of the flow path 11 is 0.01 to 1000 μm, 0.05 to 1000 μm, 0.2 to 500 μm, 1 to 250 μm, and 10 to 200 μm. Considering the formability when the first substrate 6 is manufactured by injection molding, the width of the flow path 11 is preferably 0.05 to 5 mm.
　流路１１の深さは、一例として、０．０１～１００ｍｍ、０．０５～５０ｍｍ、０．１～１０ｍｍ、０．１～５ｍｍ、０．５～３μｍが挙げられる。また、さらに一例として、流路１１の深さは、０．０１～１０００μｍ、０．０５～１０００μｍ、０．２～５００μｍ、１～２５０μｍ、１０～２００μｍが挙げられる。第１基材６を射出成形で製作する場合の成形性を考慮すると、流路１１の深さは０．０５～３ｍｍが好ましい。 Examples of the depth of the flow path 11 include 0.01 to 100 mm, 0.05 to 50 mm, 0.1 to 10 mm, 0.1 to 5 mm, and 0.5 to 3 μm. Further, as an example, the depth of the flow path 11 is 0.01 to 1000 μm, 0.05 to 1000 μm, 0.2 to 500 μm, 1 to 250 μm, and 10 to 200 μm. Considering the moldability when the first substrate 6 is manufactured by injection molding, the depth of the flow path 11 is preferably 0.05 to 3 mm.
　バルブ装置Ｖは、流路１１と対向する位置で第１基材６を上下方向に貫通するバルブ保持孔（貫通孔）３４に保持されたバルブ部Ｖｃと、第２基材９に設けられた窪み４０と、図２に示されるように、第１基材６２の上面（第１面）６ｂに剥離可能に貼り付けられるシート材４１と、バルブ部Ｖｃに設けられた軸部（付与部、駆動部）４２とを有している。バルブ部Ｖｃは、バルブ保持孔３４に設けられた環状の円環部４４の下端部に設けられている。軸部４２はバルブ部Ｖｃを変形させて駆動させる駆動部である。また、バルブ部Ｖｃである弾性部材を変形させる力を付与する付与部であり、弾性部材を押圧する押圧部であると換言できる。 The valve device V is provided in the second base material 9 and a valve portion Vc held in a valve holding hole (through hole) 34 that penetrates the first base material 6 in the vertical direction at a position facing the flow path 11. As shown in FIG. 2, the recess 40, a sheet material 41 that is detachably attached to the upper surface (first surface) 6 b of the first base material 62, and a shaft portion (giving portion, provided on the valve portion Vc) Drive part) 42. The valve portion Vc is provided at a lower end portion of an annular ring portion 44 provided in the valve holding hole 34. The shaft portion 42 is a drive portion that deforms and drives the valve portion Vc. Moreover, it can be said that it is a provision part which provides the force which deforms the elastic member which is the valve part Vc, and is a press part which presses an elastic member.
　バルブ部Ｖｃは、弾性部材である。バルブ部Ｖｃは、柔軟な樹脂の膜からなる弁である。貫通孔３４の流路１１側の開口部はバルブ部Ｖｃである弾性部材で塞がれている。バルブ部Ｖｃは弾性材料から構成され、変形により流路１１中の流体の流れを調整する。バルブ部Ｖｃに採用可能な弾性材料としては、ゴム、エラストマー樹脂などが例示される。バルブ部Ｖｃの下面には、半球状に突出する突出部３６が設けられている。第１基材６と第２基材９とが接合した際に、貫通孔の流路１１側の開口部は流路１１に接続する。バルブ部Ｖｃの貫通孔の流路１１側の開口部の面は、流路１１の壁面の一部を構成する。 The valve part Vc is an elastic member. The valve portion Vc is a valve made of a flexible resin film. The opening on the flow path 11 side of the through hole 34 is closed by an elastic member that is the valve portion Vc. The valve portion Vc is made of an elastic material and adjusts the flow of fluid in the flow path 11 by deformation. Examples of the elastic material that can be used for the valve portion Vc include rubber and elastomer resin. A projecting portion 36 projecting in a hemispherical shape is provided on the lower surface of the valve portion Vc. When the first base material 6 and the second base material 9 are joined, the opening on the flow path 11 side of the through hole is connected to the flow path 11. The surface of the opening on the flow channel 11 side of the through hole of the valve portion Vc constitutes a part of the wall surface of the flow channel 11.
　窪み４０は、第２基材９の上面９ｂにおけるバルブ部Ｖｃの直下に配置されている。窪み４０は、半球状に形成されている。バルブ部Ｖｃは、変形により流路１１を仕切って開閉する。例えば、バルブ部Ｖｃは、流路１１の流路方向に対し垂直に動作して、流路１１の開閉を行う。図２に示すように、バルブ部Ｖｃは、下側に向かって弾性変形して突出部３６が窪み４０に当接することで流路１１を閉塞する。また、バルブ部Ｖｃは、弾性復元力により突出部３６が窪み４０から離間することで、図１に示したように、流路１１を開放する。 The depression 40 is disposed immediately below the valve portion Vc on the upper surface 9 b of the second base material 9. The recess 40 is formed in a hemispherical shape. The valve part Vc opens and closes the flow path 11 by deformation. For example, the valve part Vc operates perpendicularly to the flow path direction of the flow path 11 to open and close the flow path 11. As shown in FIG. 2, the valve portion Vc is elastically deformed downward, and the projecting portion 36 comes into contact with the recess 40 to close the flow path 11. Further, the valve portion Vc opens the flow path 11 as shown in FIG. 1 by the protrusion 36 being separated from the recess 40 by the elastic restoring force.
　軸部４２は、流路１１に対して略垂直な支柱形状である。軸部４２は、上端が第１基材６の上面６ｂよりも突出する長さに形成されている。また、軸部４２の長さは、バルブ部Ｖが弾性変形して突出部３６が窪み４０に当接したときに、上端面が第１基材６２の上面６ｂと略面一、あるいは上面６ｂよりも上方に突出する長さである。すなわち、軸部４２のバルブ保持孔３４の貫通方向の長さは、シート材４１が上面６ｂに貼り付けられる前に上面６ｂから突出し、シート材４１が上面６ｂに貼り付けられた後にバルブ部Ｖｃを変形させて流路１１を閉じる長さである。 The shaft portion 42 has a pillar shape substantially perpendicular to the flow path 11. The shaft part 42 is formed in such a length that the upper end protrudes from the upper surface 6 b of the first base material 6. Further, the length of the shaft portion 42 is such that the upper end surface is substantially flush with the upper surface 6b of the first base material 62 or the upper surface 6b when the valve portion V is elastically deformed and the protruding portion 36 comes into contact with the recess 40. It is a length protruding upward. That is, the length of the shaft portion 42 in the penetrating direction of the valve holding hole 34 projects from the upper surface 6b before the sheet material 41 is attached to the upper surface 6b, and after the sheet material 41 is attached to the upper surface 6b. Is a length to close the flow path 11 by deforming.
　つまり、バルブ部Ｖｃの下面が流路１１の底面に接触したときのバルブ部Ｖｃの上面からシート材４１の下面までの高さと、付与部（駆動部）である軸部４２の高さとは、弾性変形によるバルブ部Ｖｃの変形量分を除き実質同一である。換言すると、流路１１の底面から流路１１の天井面までの高さと第１基材６の厚さとの和から、バルブ部Ｖｃの厚みを引いた差分と、軸部４２の高さは弾性変形によるバルブ部Ｖｃの変形量分を除き実質同一である。バルブ部Ｖｃ、突出部３６及び軸部４２は、上述した可撓性を有する弾性材料で一体的に成形された成形体である。 That is, the height from the upper surface of the valve portion Vc to the lower surface of the sheet material 41 when the lower surface of the valve portion Vc comes into contact with the bottom surface of the flow path 11 and the height of the shaft portion 42 that is an application portion (drive portion) are: Except for the amount of deformation of the valve portion Vc due to elastic deformation, it is substantially the same. In other words, the difference obtained by subtracting the thickness of the valve portion Vc from the sum of the height from the bottom surface of the flow channel 11 to the ceiling surface of the flow channel 11 and the thickness of the first base member 6 and the height of the shaft portion 42 are elastic. Except for the amount of deformation of the valve portion Vc due to deformation, it is substantially the same. The valve portion Vc, the protruding portion 36, and the shaft portion 42 are formed bodies that are integrally formed of the above-described flexible elastic material.
　シート材４１は、上述した検出・検査を行う前に流体デバイス１の保管、運搬時に、流路１１に収容された溶液の安定保管及び溶液の吸湿防止等に用いられる。シート材４１は、フィルム等の薄膜材で形成されている。シート材４１は、第１基材６の上面６ｂに剥離可能に貼り付けられる。シート材４１は、第１基材６の上面６ｂとの接触面側に接着層を有していてもよい。また、シート材４１は接着材を介して、第１基材６の上面６ｂに剥離可能に貼り付けられてもよい。本実施形態におけるシート材４１は、バルブ保持孔３４及び貫通孔３５ａ、３５ｂの開口部を覆う大きさを有している。 The sheet material 41 is used for stable storage of the solution stored in the flow path 11 and prevention of moisture absorption of the solution during storage and transportation of the fluid device 1 before performing the detection and inspection described above. The sheet material 41 is formed of a thin film material such as a film. The sheet material 41 is detachably attached to the upper surface 6b of the first base material 6. The sheet material 41 may have an adhesive layer on the contact surface side with the upper surface 6 b of the first base material 6. Further, the sheet material 41 may be detachably attached to the upper surface 6b of the first base material 6 via an adhesive. The sheet material 41 in the present embodiment has a size that covers the valve holding hole 34 and the openings of the through holes 35a and 35b.
　上記の流体デバイス１のうち、第１基材６、バルブ部Ｖｃ、突出部３６及び軸部４２は、一体的に成形された成形体である。第１基材６、バルブ部Ｖｃ、突出部３６及び軸部４２は、例えば、第１基材６を成形した後に、第１基材６が収容された金型を用いてバルブ部Ｖｃ、突出部３６及び軸部４２を成形する２色成形で成形される。また、予め製作した第１基材６と、バルブ部Ｖｃ、突出部３６及び軸部４２との一方を、他方を成形するための金型に装着して成形するインサート成形で成形することも可能である。 Among the fluid devices 1 described above, the first base member 6, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are integrally formed molded bodies. For example, the first base material 6, the valve portion Vc, the projecting portion 36, and the shaft portion 42 are formed using the mold in which the first base material 6 is accommodated after the first base material 6 is molded. The portion 36 and the shaft portion 42 are molded by two-color molding. Moreover, it is also possible to mold by insert molding in which one of the first base material 6 manufactured in advance, the valve portion Vc, the projecting portion 36 and the shaft portion 42 is mounted on a mold for molding the other. It is.
［システムの第１実施形態］
　図３及び図４は、上記の流体デバイス１にシート材４１を貼り付ける貼付装置６０を有するシステムの第１実施形態を示す概略構成図である。
　図３及び図４に示すように、システムは、貼付装置６０として、ローラー装置６１を有している。ローラー装置６１は、シート材４１を介して第１基材６の上面６ｂ上を転動する。 [First Embodiment of System]
3 and 4 are schematic configuration diagrams showing a first embodiment of a system having a sticking device 60 for sticking the sheet material 41 to the fluid device 1 described above.
As shown in FIGS. 3 and 4, the system includes a roller device 61 as the sticking device 60. The roller device 61 rolls on the upper surface 6 b of the first base material 6 via the sheet material 41.
　上記構成のシステムでは、第１基材６の上面６ｂ側にシート材４１を配置した後に、図３に示すように、ローラー装置６１が一方側（図３では右側）の端部から他方側（図４では左側）までシート材４１を介して上面６ｂ上を転動する。ローラー装置６１により押し付けられることにより、シート材４１は上面６ｂに貼り付けられる。シート材４１が上面６ｂに貼り付けられることにより、軸部４２は下方に押し下げられる。すなわち、シート材４１が貼り付けられることにより、軸部４２は流路において流体が流れる方向とは略垂直方向に押し込まれる。これにより、バルブ部Ｖｃは、流路において流体が流れる方向とは略垂直方向に弾性変形し、突出部３６が窪み４０に当接することで流路１１が閉塞された状態となる。 In the system configured as described above, after the sheet material 41 is disposed on the upper surface 6b side of the first base material 6, as shown in FIG. 3, the roller device 61 moves from the end portion on one side (right side in FIG. 3) to the other side ( It rolls on the upper surface 6b through the sheet material 41 until the left side in FIG. By being pressed by the roller device 61, the sheet material 41 is bonded to the upper surface 6b. By attaching the sheet material 41 to the upper surface 6b, the shaft portion 42 is pushed downward. That is, by sticking the sheet material 41, the shaft portion 42 is pushed in a direction substantially perpendicular to the direction in which the fluid flows in the flow path. As a result, the valve portion Vc is elastically deformed in a direction substantially perpendicular to the direction in which the fluid flows in the flow path, and the flow path 11 is closed by the protrusion 36 coming into contact with the recess 40.
　一方、流体デバイス１を用いて検出・検査を行う際には、シート材４１を上面６ｂから剥離する。また、貫通孔３５において、前記シート材４１に穴をあけたり破ったりする。これにより、シート材４１により軸部４２に与えられていた力が除かれる。そして、バルブ部Ｖｃは、弾性復元力により、図１に示したように、窪み４０から離間し、流路１１が開放される。 On the other hand, when performing detection / inspection using the fluid device 1, the sheet material 41 is peeled from the upper surface 6b. In addition, the sheet material 41 is pierced or broken in the through hole 35. As a result, the force applied to the shaft portion 42 by the sheet material 41 is removed. Then, the valve portion Vc is separated from the recess 40 by the elastic restoring force, as shown in FIG. 1, and the flow path 11 is opened.
　上記のシステムでは、上面６ｂに貼り付けられたシート材４１が貫通孔３５ａ、３５ｂを閉塞しているため、流路１１は大気開放されない。そのため、流路１１に収容された溶液は、当該流路１１に保持される。また、シート材４１が上面６ｂに貼り付けられた際には、バルブ部Ｖｃが流路１１を閉塞して分断する。そのため、シート材４１が貫通孔３５ａ、３５ｂの開口部を覆わない大きさで貼り付けられた場合、分断された流路１１のそれぞれは貫通孔３５ａ、３５ｂ側が大気開放された状態となる。 In the above system, since the sheet material 41 attached to the upper surface 6b closes the through holes 35a and 35b, the flow path 11 is not opened to the atmosphere. Therefore, the solution stored in the channel 11 is held in the channel 11. Further, when the sheet material 41 is attached to the upper surface 6b, the valve portion Vc closes and divides the flow path 11. Therefore, when the sheet material 41 is affixed in a size that does not cover the openings of the through holes 35a and 35b, the divided flow paths 11 are in a state where the through holes 35a and 35b are open to the atmosphere.
　この状態で分断された流路１１に収容された溶液が大気開放された側に流動する力が働いた場合、分断された各流路１１におけるバルブ部Ｖｃ側が負圧となるため、溶液は貫通孔３５ａ、３５ｂから漏れ出さず各流路１１に保持される。 In this state, when the force of flowing the solution accommodated in the divided flow path 11 to the side opened to the atmosphere works, the valve portion Vc side in each divided flow path 11 becomes negative pressure, so the solution penetrates. It does not leak from the holes 35a and 35b and is held in each flow path 11.
　また、分断された各流路１１に同じ溶液ではなく、例えば、後工程で混合される種類の異なる溶液が収容されていた場合でも、バルブ部Ｖｃによって分断された各流路１１の独立性を保持できるため、流体デバイス１を用いた検出・検査前に各流路１１に収容された溶液同士が混合されることを効果的に抑制できる。 Further, independence of each flow path 11 divided by the valve portion Vc even when the divided flow paths 11 contain not the same solution but different types of solutions mixed in a subsequent process, for example. Since it can hold | maintain, it can suppress effectively that the solution accommodated in each flow path 11 is mixed before the detection and test | inspection using the fluid device 1. FIG.
　以上のように、本実施形態では、流体デバイス１を用いた検出・検査前にシート材４１を上面６ｂに貼り付けたときに、流路１１を閉じる力をバルブ部Ｖｃに付与する軸部４２が設けられているため、バルブ部Ｖｃが初期クローズバルブ（ノーマリークローズバルブ）として作用する。すなわち、シート材４１が上面６ｂに貼り付けられたとき、バルブ部Ｖｃは流路１１中の流体の流れをせき止めた状態とする閉状態であるため、検出・検査前の保管、運搬時に流路１１に収容された溶液が漏れ出したり、混合前の溶液同士が混合されたりすることを抑制できる。また、本実施形態では、シート材４１を上面６ｂから剥離することにより、バルブ部Ｖｃは流路１１中の流体が流れる開状態へと変形し、流路１１の閉塞が解除されるため、保管、運搬後に円滑、且つ安定的に流体デバイス１を用いた検出・検査を行うことが可能である。また、本実施形態では、ローラー装置６１がシート材４１を介して上面６ｂ上を転動することにより、シート材４１を容易に上面６ｂに貼り付けることが可能である。特に、シート材４１が貫通孔３４、３５ａ、３５ｂの開口部のすべてを覆うものであった場合、簡便にシート材４１の貼り付けができる。
　なお、シート材４１は、上面６ｂ全体を覆うものではなく、貫通孔３４、３５ａ、３５ｂの上面６ｂ側の開口部を部分的に覆うものであってもよい。また、貫通孔３４、３５ａ、３５ｂの開口部を覆うシート材４１はそれぞれ別個のものであってもよい。この場合、少量のシート材で済み、また、段階的に貫通孔３４、３５ａ、３５ｂを解放することができる。 As described above, in this embodiment, when the sheet material 41 is affixed to the upper surface 6b before the detection / inspection using the fluid device 1, the shaft portion 42 that applies the force for closing the flow path 11 to the valve portion Vc. Therefore, the valve portion Vc functions as an initial close valve (normally close valve). That is, when the sheet material 41 is attached to the upper surface 6b, the valve portion Vc is in a closed state in which the flow of the fluid in the flow path 11 is blocked, so that the flow path is stored and transported before detection and inspection. It can suppress that the solution accommodated in 11 leaks, or the solutions before mixing are mixed. In the present embodiment, the sheet material 41 is peeled from the upper surface 6b, whereby the valve portion Vc is deformed into an open state in which the fluid in the flow path 11 flows, and the blockage of the flow path 11 is released. It is possible to perform detection and inspection using the fluid device 1 smoothly and stably after transportation. In the present embodiment, the roller device 61 rolls on the upper surface 6b via the sheet material 41, whereby the sheet material 41 can be easily attached to the upper surface 6b. In particular, when the sheet material 41 covers all the openings of the through holes 34, 35a, and 35b, the sheet material 41 can be easily attached.
Note that the sheet material 41 does not cover the entire upper surface 6b but may partially cover the openings on the upper surface 6b side of the through holes 34, 35a, and 35b. Further, the sheet materials 41 covering the openings of the through holes 34, 35a, and 35b may be different from each other. In this case, a small amount of sheet material is sufficient, and the through holes 34, 35a, and 35b can be released stepwise.
［システムの第２実施形態］
　次に、システムの第２実施形態について、図５及び図６を参照して説明する。
　これらの図において、図１乃至図４に示す第１実施形態の構成要素と同一の要素については同一符号を付し、その説明を省略する。
　第２実施の形態と上記の第１実施形態とが異なる点は、貼付装置６０の構成であるため、以下では貼付装置６０について説明する。 [Second Embodiment of System]
Next, a second embodiment of the system will be described with reference to FIGS.
In these drawings, the same components as those of the first embodiment shown in FIGS. 1 to 4 are denoted by the same reference numerals, and the description thereof is omitted.
Since the difference between the second embodiment and the first embodiment is the configuration of the sticking device 60, the sticking device 60 will be described below.
　図５に示すように、本実施形態では、検出・検査前の保管、運搬時に袋体６２を用いて流体デバイス１を包装する。本実施形態では、袋体６２のうち、上面６ｂと対向する領域がシート材４１である。本実施形態におけるシステムでは、図６に示すように、貼付装置６０として、吸引装置６３が設けられている。吸引装置６３は、袋体６２の開口部を介して袋体６２の内部を負圧吸引する。 As shown in FIG. 5, in this embodiment, the fluid device 1 is packaged using the bag body 62 during storage and transportation before detection and inspection. In the present embodiment, a region facing the upper surface 6 b of the bag body 62 is the sheet material 41. In the system according to the present embodiment, as shown in FIG. 6, a suction device 63 is provided as the sticking device 60. The suction device 63 sucks the inside of the bag body 62 through the opening of the bag body 62 under negative pressure.
　上記構成のシステムにおいては、吸引装置６３が袋体６２の内部を負圧吸引することにより、袋体６２の内部の気圧が外部の気圧よりも低くなる。その結果、袋体６２は、図６に示すように、上面６ｂを含む基材５の外表面に気圧により押し付けられて貼り付けられる。袋体６２が上面６ｂに貼り付けられることにより、上述したように、軸部４２は下方に押し下げられる。これにより、バルブ部Ｖｃは、突出部３６が窪み４０に当接することで流路１１が閉塞された状態となる。 In the system configured as described above, the suction device 63 sucks the inside of the bag body 62 with a negative pressure, so that the pressure inside the bag body 62 becomes lower than the outside pressure. As a result, as shown in FIG. 6, the bag body 62 is pressed and attached to the outer surface of the base material 5 including the upper surface 6 b by atmospheric pressure. By attaching the bag body 62 to the upper surface 6b, the shaft portion 42 is pushed downward as described above. As a result, the valve portion Vc is in a state in which the flow path 11 is closed by the protrusion 36 coming into contact with the recess 40.
　この後、溶着等によって袋体６２の口部に封止部６４を設けることにより、バルブ部Ｖｃによる流路１１の閉塞が保持される。また、流体デバイス１を用いて検出・検査を行う際には、封止部６４による袋体６２の封止を解除することにより、図５に示したように、バルブ部Ｖｃは、弾性復元力により窪み４０から離間し、流路１１が開放される。 Thereafter, the sealing portion 64 is provided at the mouth portion of the bag body 62 by welding or the like, whereby the blockage of the flow path 11 by the valve portion Vc is maintained. Further, when performing detection / inspection using the fluid device 1, the valve portion Vc has an elastic restoring force as shown in FIG. Thus, the channel 11 is released from the recess 40 and opened.
　このように、本実施形態のシステムでは、上記第１実施形態のシステムと同様の作用・効果が得られることに加えて、検出・検査前に流体デバイス１が袋体６２で包装されるため、検出・検査前に流体デバイス１が塵埃等で汚染されることを抑制できる。 As described above, in the system of the present embodiment, in addition to obtaining the same operation and effect as the system of the first embodiment, the fluid device 1 is packaged by the bag body 62 before detection and inspection. Contamination of the fluid device 1 with dust or the like before detection / inspection can be suppressed.
［流体デバイスの第２実施形態及び検出装置］
　次に、流体デバイスの第２実施形態及び検出装置について、図７及び図８を参照して説明する。
　これらの図において、図１及び図２に示す第１実施形態の構成要素と同一の要素については同一符号を付し、その説明を省略する。 [Second Embodiment and Detection Device of Fluidic Device]
Next, a fluid device according to a second embodiment and a detection apparatus will be described with reference to FIGS.
In these drawings, the same components as those of the first embodiment shown in FIGS. 1 and 2 are denoted by the same reference numerals, and the description thereof is omitted.
　図７は、第２実施形態に係る流体デバイス１を用いて検出を行う検出装置７０の断面模式図である。図８は、流体デバイス１を模式的に示した平面図である。なお、図８においては、透明な第１基材６について、下側に配置された各部を透過させた状態で図示する。 FIG. 7 is a schematic cross-sectional view of a detection apparatus 70 that performs detection using the fluidic device 1 according to the second embodiment. FIG. 8 is a plan view schematically showing the fluidic device 1. In addition, in FIG. 8, it illustrates in the state which permeate | transmitted each part arrange | positioned about the transparent 1st base material 6 below.
　検出装置７０は、流体デバイス１を載置する載置部７１と、シート材４１を剥離する剥離部７２と、流体デバイス１の流路１１に対象物質を含む溶液を送液する送液部７３と、溶液中の対象物質を検出する検出部７４とを備える。 The detection device 70 includes a placement unit 71 for placing the fluid device 1, a peeling unit 72 for peeling the sheet material 41, and a liquid feeding unit 73 for feeding a solution containing the target substance to the flow path 11 of the fluid device 1. And a detection unit 74 that detects a target substance in the solution.
　図８に示すように、流体デバイス１は、基材５を備える。また、図７に示すように、基材５は、厚さ方向に積層された３つの基板（第１基材６、第２基材９および第３基材８）を有する。第３基材８は、第１基材６及び第２基材９と同様の樹脂材で形成され、第２基材９の下側に配置されている。第３基材８の上面８ｂは、第２基材９の下面９ａと接合されている。 As shown in FIG. 8, the fluid device 1 includes a base material 5. Moreover, as shown in FIG. 7, the base material 5 has three board | substrates (the 1st base material 6, the 2nd base material 9, and the 3rd base material 8) laminated | stacked on the thickness direction. The third base material 8 is formed of the same resin material as the first base material 6 and the second base material 9, and is disposed below the second base material 9. The upper surface 8 b of the third base material 8 is joined to the lower surface 9 a of the second base material 9.
　基材５には、注入孔３２と、リザーバー２９と、流路１１と、廃液槽７と、空気孔３５と、供給孔３９と、定量バルブＶｑ、導入バルブＶｉ、排出バルブＶｏと上述したバルブ装置Ｖとが設けられている。 The base material 5 includes an injection hole 32, a reservoir 29, a flow path 11, a waste liquid tank 7, an air hole 35, a supply hole 39, a metering valve Vq, an introduction valve Vi, a discharge valve Vo, and the valves described above. A device V is provided.
　注入孔３２は、第１基材６および第２基材９を貫通する。注入孔３２は、第２基材９と第３基材８との境界部に位置するリザーバー２９に繋がる。注入孔３２は、リザーバー２９を外部に繋げる。溶液は、注入孔３２を介してリザーバー２９に充填される。注入孔３２は、１つのリザーバー２９に対して１つ設けられる。なお、図８において、注入孔３２の図示は、省略されている。 The injection hole 32 penetrates the first base material 6 and the second base material 9. The injection hole 32 is connected to a reservoir 29 located at the boundary between the second base material 9 and the third base material 8. The injection hole 32 connects the reservoir 29 to the outside. The solution is filled into the reservoir 29 through the injection hole 32. One injection hole 32 is provided for one reservoir 29. In FIG. 8, the injection hole 32 is not shown.
　リザーバー２９は、少なくとも前記流路１１との接続部においては、流路形状である。例えば、第２基材９の下面９ａに設けられたチューブ状、あるいは筒状に形成された空間である。本実施形態の基材５には、複数のリザーバー２９が設けられる。リザーバー２９には、溶液が収容される。複数のリザーバー２９は、互いに独立して溶液を収容する。複数のリザーバー２９は、例えば、複数の試薬を収容する。複数のリザーバー２９のそれぞれには、上述した初期クローズバルブが配置されている。リザーバー２９は、収容した溶液を流路１１に供給する。本実施形態のリザーバー２９は、一例として、流路型のリザーバーである。リザーバー２９の長さ方向の一端は、注入孔３２に接続されている。また、リザーバー２９の長さ方向の他端は、供給孔３９が接続されている。 The reservoir 29 has a flow channel shape at least in the connection portion with the flow channel 11. For example, it is a space formed in a tubular shape or a cylindrical shape provided on the lower surface 9 a of the second base material 9. The substrate 5 of the present embodiment is provided with a plurality of reservoirs 29. The reservoir 29 stores a solution. The plurality of reservoirs 29 contain solutions independently of each other. The plurality of reservoirs 29 contain, for example, a plurality of reagents. Each of the plurality of reservoirs 29 is provided with the above-described initial close valve. The reservoir 29 supplies the stored solution to the flow path 11. The reservoir 29 of this embodiment is a flow path type reservoir as an example. One end of the reservoir 29 in the length direction is connected to the injection hole 32. Further, the supply hole 39 is connected to the other end of the reservoir 29 in the length direction.
　なお、本実施形態では、リザーバー２９が第２基材９に設けられ第３基材８によって開口部が覆われる構成について説明した。しかしながら、リザーバー２９は、第３基材８に設けられ開口部を第２基材９により覆う構成であってもよい。 In the present embodiment, the configuration in which the reservoir 29 is provided on the second base material 9 and the opening is covered with the third base material 8 has been described. However, the reservoir 29 may be configured to be provided in the third base material 8 and cover the opening with the second base material 9.
　流路１１は、第２基材９の上面９ｂに設けられたチューブ状、あるいは筒状に形成された空間である。流路１１には、リザーバー２９から供給孔３９を介して溶液が供給される。溶液は、流路１１内を流れる。 The flow path 11 is a space formed in a tube shape or a cylindrical shape provided on the upper surface 9 b of the second base material 9. The solution is supplied to the flow path 11 from the reservoir 29 through the supply hole 39. The solution flows in the flow path 11.
　供給孔３９は、第２基材９に設けられている。供給孔３９は、第２基材９を板厚方向に貫通する。供給孔３９は、リザーバー２９と流路１１とを繋ぐ。リザーバー２９に貯留された溶液は、供給孔３９を介して流路１１に供給される。すなわち、リザーバー２９は、供給孔３９を介して流路１１と接続されている。 The supply hole 39 is provided in the second base material 9. The supply hole 39 penetrates the second base material 9 in the plate thickness direction. The supply hole 39 connects the reservoir 29 and the flow path 11. The solution stored in the reservoir 29 is supplied to the flow path 11 through the supply hole 39. That is, the reservoir 29 is connected to the flow path 11 through the supply hole 39.
　廃液槽７は、流路１１中の溶液を廃棄する為に基材５に設けられる。廃液槽７は、流路１１に接続される。図８に示すように、廃液槽７は、循環流路１０の内側領域に配置されている。これにより、流体デバイス１の小型化を図ることができる。また、図７に示すように、廃液槽７は、第２基材９の上面９ｂ側に設けられた凹部ぬいより構成される。 The waste liquid tank 7 is provided on the base material 5 in order to discard the solution in the flow path 11. The waste liquid tank 7 is connected to the flow path 11. As shown in FIG. 8, the waste liquid tank 7 is disposed in the inner region of the circulation channel 10. Thereby, size reduction of the fluid device 1 can be achieved. Further, as shown in FIG. 7, the waste liquid tank 7 is constituted by a concave stitch provided on the upper surface 9 b side of the second base material 9.
　空気孔３５は、第１基材６に設けられる。空気孔３５は、廃液槽７の直上に位置する。空気孔３５は、廃液槽７を外部に繋げる。すなわち、廃液槽７は、空気孔３５を介して外部に開放される。 The air hole 35 is provided in the first base material 6. The air hole 35 is located immediately above the waste liquid tank 7. The air hole 35 connects the waste liquid tank 7 to the outside. That is, the waste liquid tank 7 is opened to the outside through the air hole 35.
　図７に示すように、導入バルブＶｉは、供給孔３９と廃液槽７との間における流路１１と対向する位置に配置されている。導入バルブＶｉは、流路１１と対向する位置で第１基材６を上下方向に貫通するバルブ保持孔３４ｉに保持されたバルブ部Ｖｄを有している。バルブ部Ｖｄは、ゴム、エラストマー樹脂などの弾性材料で形成されている。バルブ部Ｖｄの下面には、半球状に突出する突出部３６ｉが設けられている。バルブ部Ｖｄと対向する第２基材９には、窪み４０ｉが設けられている。バルブ部Ｖｄは、バルブ保持孔３４ｉに駆動流体（例えば、空気）が供給されることにより、下側に弾性変形して流路１１中の溶液の流れを調整する。バルブ部Ｖｄは、バルブ保持孔３４ｉに駆動流体が供給されて下側に弾性変形し、突出部３６ｉが窪み４０ｉに当接することにより流路１１を閉じる。バルブ部Ｖｄは、バルブ保持孔３４ｉへの駆動流体の供給が停止されたときに、弾性復元力で突出部３６ｉが窪み４０ｉから離間することにより流路１１を開放する。 As shown in FIG. 7, the introduction valve Vi is disposed at a position facing the flow path 11 between the supply hole 39 and the waste liquid tank 7. The introduction valve Vi has a valve portion Vd held in a valve holding hole 34 i that penetrates the first base material 6 in the vertical direction at a position facing the flow path 11. The valve portion Vd is formed of an elastic material such as rubber or elastomer resin. A projecting portion 36i projecting in a hemispherical shape is provided on the lower surface of the valve portion Vd. A recess 40i is provided in the second base material 9 facing the valve portion Vd. The valve portion Vd is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34i, and adjusts the flow of the solution in the flow path 11. The valve portion Vd is supplied with driving fluid into the valve holding hole 34i and elastically deforms downward, and the projecting portion 36i contacts the recess 40i to close the flow path 11. When the supply of the driving fluid to the valve holding hole 34i is stopped, the valve portion Vd opens the flow path 11 by separating the protruding portion 36i from the recess 40i by an elastic restoring force.
　排出バルブＶｏは、導入バルブＶｉと廃液槽７との間における流路１１と対向する位置に配置されている。排出バルブＶｏは、流路１１と対向する位置で第１基材６を上下方向に貫通するバルブ保持孔３４ｏに保持されたバルブ部Ｖｆを有している。バルブ部Ｖｆは、ゴム、エラストマー樹脂などの弾性材料で形成されている。バルブ部Ｖｆの下面には、半球状に突出する突出部３６ｏが設けられている。バルブ部Ｖｆと対向する第２基材９には、窪み４０ｏが設けられている。バルブ部Ｖｆは、バルブ保持孔３４ｏに駆動流体（例えば、空気）が供給されることにより、下側に弾性変形して流路１１中の溶液の流れを調整する。バルブ部Ｖｆは、バルブ保持孔３４ｏに駆動流体が供給されて下側に弾性変形し、突出部３６ｏが窪み４０ｏに当接することにより流路１１を閉じる。バルブ部Ｖｆは、バルブ保持孔３４ｉへの駆動流体の供給が停止されたときに、弾性復元力で突出部３６ｉが窪み４０ｏから離間することにより流路１１を開放する。 The discharge valve Vo is disposed at a position facing the flow path 11 between the introduction valve Vi and the waste liquid tank 7. The discharge valve Vo has a valve portion Vf held in a valve holding hole 34o penetrating the first base material 6 in the vertical direction at a position facing the flow path 11. The valve portion Vf is formed of an elastic material such as rubber or elastomer resin. A projecting portion 36o projecting in a hemispherical shape is provided on the lower surface of the valve portion Vf. The second base material 9 facing the valve portion Vf is provided with a recess 40o. The valve portion Vf is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34o and adjusts the flow of the solution in the flow path 11. The valve portion Vf is supplied with driving fluid into the valve holding hole 34o and elastically deforms downward, and the projecting portion 36o contacts the recess 40o to close the flow path 11. When the supply of the driving fluid to the valve holding hole 34i is stopped, the valve portion Vf opens the flow path 11 by separating the protruding portion 36i from the recess 40o by an elastic restoring force.
　定量バルブＶｑは、流路１１と対向する位置で第１基材６を上下方向に貫通するバルブ保持孔３４ｑに保持されたバルブ部Ｖｅを有している。バルブ部Ｖｅは、ゴム、エラストマー樹脂などの弾性材料で形成されている。バルブ部Ｖｅの下面には、半球状に突出する突出部３６ｑが設けられている。バルブ部Ｖｅと対向する第２基材９には、窪み４０ｑが設けられている。バルブ部Ｖｅは、バルブ保持孔３４ｑに駆動流体（例えば、空気）が供給されることにより、下側に弾性変形して流路１１中の溶液の流れを調整する。バルブ部Ｖｅは、バルブ保持孔３４ｑに駆動流体が供給されて下側に弾性変形し、突出部３６ｑが窪み４０ｑに当接することにより流路１１を閉じる。バルブ部Ｖｅは、バルブ保持孔３４ｑへの駆動流体の供給が停止されたときに、弾性復元力で突出部３６ｑが窪み４０ｑから離間することにより流路１１を開放する。 The metering valve Vq has a valve portion Ve held by a valve holding hole 34q that penetrates the first base material 6 in a vertical direction at a position facing the flow path 11. The valve portion Ve is formed of an elastic material such as rubber or elastomer resin. A projecting portion 36q projecting in a hemispherical shape is provided on the lower surface of the valve portion Ve. The second base material 9 facing the valve portion Ve is provided with a recess 40q. The valve portion Ve is elastically deformed downward by supplying a driving fluid (for example, air) to the valve holding hole 34q, and adjusts the flow of the solution in the flow path 11. The valve portion Ve is supplied with driving fluid into the valve holding hole 34q and elastically deforms downward, and the projecting portion 36q contacts the recess 40q to close the flow path 11. When the supply of the driving fluid to the valve holding hole 34q is stopped, the valve portion Ve opens the flow path 11 by separating the protruding portion 36q from the recess 40q by an elastic restoring force.
　上述したバルブ装置Ｖは、供給孔３９と導入バルブＶｉとの間における流路１１と対向する位置に配置されている。 The valve device V described above is disposed at a position facing the flow path 11 between the supply hole 39 and the introduction valve Vi.
　上記の流体デバイス１のうち、第１基材６、導入バルブＶｉ、排出バルブＶｏ、定量バルブＶｑ、バルブ部Ｖｃ、突出部３６及び軸部４２は、一体的に成形された成形体である。第１基材６、導入バルブＶｉ、排出バルブＶｏ、定量バルブＶｑ、バルブ部Ｖｃ、突出部３６及び軸部４２は、例えば、第１基材６を成形した後に、第１基材６が収容された金型を用いて導入バルブＶｉ、排出バルブＶｏ、定量バルブＶｑ、バルブ部Ｖｃ、突出部３６及び軸部４２を成形する２色成形で成形される。また、予め製作した第１基材６と、導入バルブＶｉ、排出バルブＶｏ、定量バルブＶｑ、バルブ部Ｖｃ、突出部３６及び軸部４２との一方を、他方を成形するための金型に装着して成形するインサート成形で成形することも可能である。 Among the fluid devices 1, the first base material 6, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are formed integrally. The first base material 6, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36 and the shaft portion 42 are accommodated in the first base material 6 after the first base material 6 is formed, for example. The introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are molded by the two-color molding using the molded mold. In addition, one of the first base 6 manufactured in advance, the introduction valve Vi, the discharge valve Vo, the metering valve Vq, the valve portion Vc, the protruding portion 36 and the shaft portion 42 is mounted on a mold for molding the other. It is also possible to mold by insert molding.
　上記の導入バルブＶｉ、排出バルブＶｏ、定量バルブＶｑは、初期オープンバルブ（第２バルブ）である。上述した初期クローズバルブと初期オープンバルブとは、流路１１において直列に並んでいる。 The introduction valve Vi, the discharge valve Vo, and the metering valve Vq are initial open valves (second valves). The initial close valve and the initial open valve described above are arranged in series in the flow path 11.
　上記の流体デバイス１においては、検出・検査前に、リザーバー２９に溶液が収容された状態で上面６ｂにシート材４１が貼り付けられる。また、図７においては、導入バルブＶｉと排出バルブＶｏとの間の流路１１には、一例として、高粘度の試薬が収容されている。上面６ｂにシート材４１が貼り付けられることにより、バルブ装置Ｖにおいて突出部３６が窪み４０に当接し流路１１が閉じられる。 In the fluid device 1, the sheet material 41 is attached to the upper surface 6b in a state where the solution is stored in the reservoir 29 before detection and inspection. In FIG. 7, a high-viscosity reagent is accommodated in the flow path 11 between the introduction valve Vi and the discharge valve Vo as an example. By sticking the sheet material 41 to the upper surface 6b, the protruding portion 36 contacts the recess 40 in the valve device V, and the flow path 11 is closed.
　上記シート材４１が貼り付けられて流路１１が突出部３６と窪み４０との当接により閉じられた流体デバイス１では、シート材４１により注入孔３２及び空気孔３５が閉塞されているため、流路１１及びリザーバー２９は大気開放されない状態となる。そのため、リザーバー２９に収容された溶液は、流動を抑制されて当該リザーバー２９に保持され、流路１１に収容された試薬は、流動を抑制されて当該流路１１に保持される。また、バルブ部Ｖｃにおいて突出部３６が窪み４０に当接し流路１１が閉じられ、リザーバー２９と流路１１とが分断されているため、流体デバイス１に大きな加速度が働いた場合でも、溶液と試薬とが混合されてしまうことを抑制できる。さらに、上記の流体デバイス１では、リザーバー２９及び流路１１における空気の流動も抑制されるため、溶液及び試薬の蒸発も抑えることが可能となる。 In the fluid device 1 in which the sheet material 41 is attached and the flow path 11 is closed by the contact between the projecting portion 36 and the recess 40, the injection hole 32 and the air hole 35 are closed by the sheet material 41. The flow path 11 and the reservoir 29 are not released to the atmosphere. Therefore, the solution stored in the reservoir 29 is held in the reservoir 29 with the flow suppressed, and the reagent stored in the flow path 11 is held in the flow path 11 with the flow suppressed. Further, in the valve portion Vc, the projecting portion 36 abuts the recess 40, the flow path 11 is closed, and the reservoir 29 and the flow path 11 are separated. Therefore, even when a large acceleration is applied to the fluid device 1, It can suppress mixing with a reagent. Furthermore, in the fluid device 1 described above, since the air flow in the reservoir 29 and the flow path 11 is also suppressed, it is possible to suppress the evaporation of the solution and the reagent.
　また、上記流体デバイス１において、シート材４１が注入孔３２及び空気孔３５の開口部を覆わない場合でも、突出部３６と窪み４０との当接により流路１１が閉じられた側のリザーバー２９及び流路１１は大気開放されていないため、リザーバー２９及び流路１１における溶液、試薬及び空気の流動が抑制される。 Further, in the fluid device 1, even when the sheet material 41 does not cover the openings of the injection hole 32 and the air hole 35, the reservoir 29 on the side where the flow path 11 is closed by the contact between the protrusion 36 and the recess 40. In addition, since the channel 11 is not open to the atmosphere, the flow of the solution, reagent, and air in the reservoir 29 and the channel 11 is suppressed.
　次に、流路１１について、より具体的に説明する。
　図８に示すように、流路１１は、循環流路１０と、複数（図８の例では３つ）の導入流路１２と、複数（図８の例では３つ）の排出流路１３と、を含む。流路１１には、リザーバー２９（図７参照）から溶液が導入される。 Next, the flow path 11 will be described more specifically.
As shown in FIG. 8, the flow path 11 includes a circulation flow path 10, a plurality (three in the example of FIG. 8) introduction flow paths 12, and a plurality (three in the example of FIG. 8) discharge flow paths 13. And including. A solution is introduced into the channel 11 from a reservoir 29 (see FIG. 7).
　循環流路１０は、積層方向から見て、ループ状に構成される。循環流路１０の経路中には、ポンプＰが配置されている。ポンプＰは、流路中に並んで配置された３つの要素ポンプＰｅから構成されている。要素ポンプＰｅは、いわゆるバルブポンプである。ポンプＰは、３つの要素ポンプＰｅを順次開閉することにより、循環流路内において液体を搬送することができる。ポンプＰを構成する要素ポンプＰｅの数は、４以上であってもよい。 The circulation channel 10 is configured in a loop shape when viewed from the stacking direction. A pump P is disposed in the path of the circulation channel 10. The pump P is composed of three element pumps Pe arranged side by side in the flow path. The element pump Pe is a so-called valve pump. The pump P can convey the liquid in the circulation channel by sequentially opening and closing the three element pumps Pe. The number of element pumps Pe constituting the pump P may be four or more.
　循環流路１０の経路中には、複数（図８の例では３つ）の定量バルブＶｑが設けられる。複数の定量バルブＶｑは、循環流路１０を複数の定量区画１８に区画する。複数の定量バルブＶｑは、それぞれの定量区画１８が所定の体積となるように配置されている。本実施形態において、３つの定量区画１８のうち、１つの定量区画には、蛇行部１８ａが設けられている。蛇行部１８ａは、左右に蛇行して形成された流路である。蛇行部１８ａは、１つの定量区画１８を所望の容量とするために設けられる。 A plurality of (three in the example of FIG. 8) metering valves Vq are provided in the path of the circulation channel 10. The plurality of metering valves Vq partitions the circulation channel 10 into a plurality of metering sections 18. The plurality of metering valves Vq are arranged so that each metering section 18 has a predetermined volume. In the present embodiment, a meandering portion 18 a is provided in one of the three quantitative sections 18. The meandering portion 18a is a channel formed by meandering left and right. The meandering portion 18a is provided in order to make one quantitative section 18 have a desired capacity.
　定量区画１８は、それぞれ流路状に延びる。複数の定量区画１８は、それぞれ、流路状の移送流路８０と、移送流路８０の一方の端部に位置する流入部８１と、移送流路８０の他方の端部に設けられる合流部８５と、を有する。したがって、それぞれの定量区画１８において、移送流路８０は、流入部８１と、合流部８５と、の間に位置する。 Quantitative sections 18 each extend in a flow path shape. The plurality of fixed-quantity sections 18 each have a flow path-shaped transfer flow path 80, an inflow portion 81 located at one end of the transfer flow path 80, and a merge portion provided at the other end of the transfer flow path 80. 85. Therefore, in each quantitative section 18, the transfer flow path 80 is located between the inflow portion 81 and the merging portion 85.
　１つの定量区画１８の流入部８１は、他の定量区画１８の合流部８５と、定量バルブＶｑを介して繋がる。また、流入部８１には、導入バルブＶｉを介して、導入流路１２が繋がる。
　同様に、１つの定量区画１８の合流部８５は、他の定量区画１８の流入部８１と、定量バルブＶｑを介して繋がる。また、合流部８５には、排出バルブＶｏを介して、排出流路１３が繋がる。 The inflow portion 81 of one quantitative section 18 is connected to the merging section 85 of another quantitative section 18 via a quantitative valve Vq. Further, the introduction flow path 12 is connected to the inflow portion 81 via the introduction valve Vi.
Similarly, the merging section 85 of one quantitative section 18 is connected to an inflow section 81 of another quantitative section 18 via a quantitative valve Vq. Moreover, the discharge flow path 13 is connected to the junction 85 via a discharge valve Vo.
　導入流路１２は、循環流路１０の定量区画１８に溶液を導入するための流路である。導入流路１２は、循環流路１０の定量区画１８毎に設けられる。導入流路１２は、一端側において供給孔３９に接続される。また、導入流路１２は、他端側において、循環流路１０の流入部８１に接続される。 The introduction flow path 12 is a flow path for introducing the solution into the quantitative section 18 of the circulation flow path 10. The introduction channel 12 is provided for each quantitative section 18 of the circulation channel 10. The introduction channel 12 is connected to the supply hole 39 on one end side. The introduction flow path 12 is connected to the inflow portion 81 of the circulation flow path 10 on the other end side.
　排出流路１３は、循環流路１０の定量区画１８の溶液を廃液槽７に排出するための流路である。排出流路１３は、循環流路１０の定量区画１８毎に設けられる。排出流路１３は、一端側において廃液槽７に接続される。また、排出流路１３は、他端側において、循環流路１０の合流部８５に接続される。 The discharge channel 13 is a channel for discharging the solution in the quantitative section 18 of the circulation channel 10 to the waste liquid tank 7. The discharge channel 13 is provided for each quantitative section 18 of the circulation channel 10. The discharge channel 13 is connected to the waste liquid tank 7 at one end side. Further, the discharge flow channel 13 is connected to the merging portion 85 of the circulation flow channel 10 on the other end side.
（検出装置７０により溶液中の対象物質を検出する手順）
　次に、検出装置７０により上記流路１１中における溶液中の対象物質を検出する手順について説明する。
　当該手順は、上述したように、リザーバー２９に溶液が収容された状態で上面６ｂにシート材４１が貼り付けられた流体デバイス１を載置部７１に載置することと、流体デバイス１からシート材４１を剥離することと、流体デバイス１においてリザーバー２９に収容された溶液を流路１１に送液することと、流路１１における溶液中の対象物質を検出することとを含む。 (Procedure for detecting the target substance in the solution by the detection device 70)
Next, a procedure for detecting the target substance in the solution in the flow path 11 by the detection device 70 will be described.
As described above, the procedure is such that the fluid device 1 having the sheet material 41 attached to the upper surface 6b in a state where the solution is stored in the reservoir 29 is placed on the placement portion 71, and the fluid device 1 to the sheet. Peeling the material 41, feeding the solution contained in the reservoir 29 in the fluid device 1 to the flow path 11, and detecting the target substance in the solution in the flow path 11.
　流体デバイス１を載置部７１に載置することにおいては、流体デバイス１における第３基材８の下面８ａが載置部７１の上面に接するように位置させる。 In placing the fluid device 1 on the placement part 71, the fluid device 1 is positioned so that the lower surface 8 a of the third base material 8 in the fluid device 1 is in contact with the upper surface of the placement part 71.
　流体デバイス１からシート材４１を剥離することにおいては、剥離部７２がシート材４１の一端を、例えば負圧吸引した状態で第１基材６の上面６ｂに対して相対移動することにより、シート材４１を上面６ｂから剥離する。このとき、載置工程において、例えば流体デバイス１における第３基材８の下面８ａが載置部７１の上面に接合していたり、流体デバイス１が載置部７１に固定されていたりする場合には剥離が容易となる。 In peeling the sheet material 41 from the fluid device 1, the peeling portion 72 moves relative to the upper surface 6 b of the first base material 6 with one end of the sheet material 41 sucked, for example, under a negative pressure. The material 41 is peeled from the upper surface 6b. At this time, in the placement step, for example, when the lower surface 8a of the third base material 8 in the fluid device 1 is bonded to the upper surface of the placement portion 71 or the fluid device 1 is fixed to the placement portion 71. Is easy to peel.
（リザーバーから流路に溶液を供給する手順）
　流体デバイス１においてリザーバー２９に収容された溶液Ｓを流路１１に送液することにおいては、流体デバイス１からシート材４１を剥離した後に、送液部７３により行われる。 (Procedure for supplying solution from reservoir to channel)
In the fluid device 1, the solution S stored in the reservoir 29 is sent to the flow path 11 by the liquid feeding unit 73 after the sheet material 41 is peeled from the fluid device 1.
　なお、後述するバルブＶｑ、Ｖｉ、Ｖｏの開閉については、上述したように、バルブ保持孔３４ｑ、３４ｉ、３４ｏへの駆動流体の供給及び供給停止を切り替えることにより行われるが、以下ではその説明を省略する。 In addition, as described above, the opening and closing of the valves Vq, Vi, and Vo, which will be described later, is performed by switching the supply and stop of the supply of the driving fluid to the valve holding holes 34q, 34i, and 34o. Omitted.
　図７に示すように、リザーバー２９には、予め溶液Ｓが保持、及び／又は、充填されている。流体デバイス１を用いた測定（検出）では、まず、シート材４１を第１基材６の上面６ｂから剥離することにより、バルブ装置Ｖにおける突出部３６が窪み４０と離間して流路１１が開放される。上述したように、シート材４１の剥離前には、リザーバー２９及び流路１１における溶液、試薬及び空気の流動が抑制されていたため、溶液、試薬は蒸発及び混合が抑えられた状態である。 As shown in FIG. 7, the reservoir 29 is preliminarily held and / or filled with the solution S. In measurement (detection) using the fluid device 1, first, the sheet material 41 is peeled from the upper surface 6 b of the first base 6, whereby the protrusion 36 in the valve device V is separated from the recess 40 and the flow path 11 is formed. Opened. As described above, since the flow of the solution, reagent, and air in the reservoir 29 and the flow path 11 is suppressed before the sheet material 41 is peeled off, the solution and the reagent are in a state in which evaporation and mixing are suppressed.
　次いで、リザーバー２９内の溶液Ｓを流路１１に移動させる。より具体的には、循環流路１０のそれぞれの定量区画１８にリザーバー２９から溶液Ｓを順番に導入する。ここでは、１つの定量区画１８に溶液Ｓを導入する手順を説明するが、他の定量区画１８についても、同様の手順を行うことで、溶液Ｓが導入される。 Next, the solution S in the reservoir 29 is moved to the flow path 11. More specifically, the solution S is sequentially introduced from the reservoir 29 into each quantitative section 18 of the circulation channel 10. Here, the procedure for introducing the solution S into one quantitative section 18 will be described, but the solution S is also introduced by performing the same procedure for the other quantitative sections 18.
　定量区画１８に溶液Ｓを導入する際のバルブＶｑ、Ｖｉ、Ｖｏの開閉について図８を基に説明する。まず、溶液Ｓを導入する定量区画１８の長さ方向両側に位置する一対の定量バルブＶｑを閉じる。さらに、該当する定量区画１８に繋がる排出流路１３の排出バルブＶｏを開くと共に、他の排出流路１３の排出バルブＶｏを閉じる。また、該当する定量区画１８に繋がる導入流路１２の導入バルブＶｉを開く。 The opening / closing of the valves Vq, Vi, Vo when introducing the solution S into the quantitative section 18 will be described with reference to FIG. First, the pair of metering valves Vq located on both sides in the length direction of the metering section 18 for introducing the solution S are closed. Further, the discharge valve Vo of the discharge flow path 13 connected to the corresponding quantitative section 18 is opened, and the discharge valves Vo of the other discharge flow paths 13 are closed. Further, the introduction valve Vi of the introduction flow path 12 connected to the corresponding quantitative section 18 is opened.
　リザーバー２９から流路１１に溶液を移動させる手順について図７を基に説明する。送液部７３としての吸引装置を用いて、空気孔３５から廃液槽７内を負圧吸引する。これにより、リザーバー２９内の溶液Ｓは、供給孔３９を介して流路１１側に移動して送液される。また、リザーバー２９の溶液Ｓの後方には、注入孔３２を通過した空気が導入される。これにより、リザーバー２９に収容された溶液Ｓは、供給孔３９、導入流路１２を介して、循環流路１０の定量区画１８に導入される。このとき、バルブ装置Ｖにおいては、流路１１が開放されているため、溶液Ｓは支障なく循環流路１０の定量区画１８に導入される。 The procedure for moving the solution from the reservoir 29 to the flow path 11 will be described with reference to FIG. Using the suction device as the liquid feeding unit 73, the inside of the waste liquid tank 7 is suctioned from the air hole 35 by negative pressure. As a result, the solution S in the reservoir 29 moves to the flow channel 11 side via the supply hole 39 and is fed. In addition, air that has passed through the injection hole 32 is introduced behind the solution S in the reservoir 29. As a result, the solution S stored in the reservoir 29 is introduced into the quantitative section 18 of the circulation channel 10 via the supply hole 39 and the introduction channel 12. At this time, in the valve device V, since the flow path 11 is opened, the solution S is introduced into the fixed quantity section 18 of the circulation flow path 10 without any trouble.
　（流路内の溶液を混合する手順）
　次に、流体デバイス１の流路１１に供給された溶液を混合する手順について図８を基に説明する。まず、上述したように循環流路１０のそれぞれの定量区画１８に溶液を導入した状態で、排出バルブＶｏおよび導入バルブＶｉを閉じ、定量バルブＶｑを開く。さらに、ポンプＰを用いて循環流路１０内の溶液を送液して循環させる。循環流路１０を循環する溶液は、流路内の流路壁面と溶液の相互作用（摩擦）により、壁面周辺の流速は遅く、流路中央の流速は速くなる。その結果、溶液の流速に分布ができるため、溶液の混合および反応が促進される。 (Procedure for mixing the solution in the flow path)
Next, the procedure for mixing the solution supplied to the flow path 11 of the fluidic device 1 will be described with reference to FIG. First, as described above, with the solution introduced into each quantitative section 18 of the circulation channel 10, the discharge valve Vo and the introduction valve Vi are closed, and the quantitative valve Vq is opened. Further, the solution in the circulation channel 10 is sent and circulated using the pump P. The solution circulating in the circulation channel 10 has a low flow velocity around the wall surface and a high flow velocity in the center of the flow channel due to the interaction (friction) between the flow channel wall surface and the solution in the flow channel. As a result, since the flow rate of the solution can be distributed, the mixing and reaction of the solution is promoted.
　流路１１における溶液中の対象物質を検出することにおいては、上記流路１１において混合された溶液を、図７に示した検出部７４が検出することにより行われる。対象物質としては、例えば、検体試料に含まれる試料物質である。試料物質は、例えば、核酸、ＤＮＡ、ＲＮＡ、ペプチド、タンパク質、細胞外小胞体などの生体分子や粒子などである。また、溶液において、対象物質と混合される物質は、試料物質に結合させて試料物質の検出を補助する標識物質（検出補助物質）、溶液中の試料物質を捕捉・収集する物質等が挙げられる。 In the detection of the target substance in the solution in the flow path 11, the detection unit 74 shown in FIG. 7 detects the solution mixed in the flow path 11. The target substance is, for example, a sample substance included in a specimen sample. The sample substance is, for example, a biomolecule such as a nucleic acid, DNA, RNA, peptide, protein, extracellular vesicle or particle. Examples of substances mixed with the target substance in the solution include a labeling substance (detection auxiliary substance) that binds to the sample substance and assists the detection of the sample substance, and a substance that captures and collects the sample substance in the solution. .
　検出部７４としては、例えば、撮像素子により第１基材６を介して対象物質を撮像して検出する構成や、第１基材６を介して磁力を測定することにより対象物質を検出する構成を採ることができる。 As the detection unit 74, for example, a configuration in which the target substance is imaged and detected via the first base material 6 by an imaging element, or a configuration in which the target substance is detected by measuring the magnetic force via the first base material 6 Can be taken.
　以上のように、本実施形態の流体デバイス１においては、上記第１実施形態の流体デバイス１と同様の作用・効果が得られることに加えて、定量バルブＶｑ、導入バルブＶｉ、排出バルブＶｏ、バルブ部Ｖｃ、突出部３６、軸部４２を２色成形により一括的に成形可能であるため、製造効率の向上を図ることが可能になる。なお、定量バルブＶｑ、導入バルブＶｉ、排出バルブＶｏ、バルブ部Ｖｃ、突出部３６、軸部４２を２色成形により一括的に成形する他に、第１基材６を成形する金型に、予め成形した定量バルブＶｑ、導入バルブＶｉ、排出バルブＶｏ、バルブ部Ｖｃ、突出部３６、軸部４２を収容した状態で成形を行うインサート成形であってもよい。 As described above, in the fluid device 1 of the present embodiment, in addition to obtaining the same operations and effects as the fluid device 1 of the first embodiment, the metering valve Vq, the introduction valve Vi, the discharge valve Vo, Since the valve portion Vc, the projecting portion 36, and the shaft portion 42 can be collectively formed by two-color molding, it is possible to improve manufacturing efficiency. In addition to forming the metering valve Vq, the introduction valve Vi, the discharge valve Vo, the valve portion Vc, the protruding portion 36, and the shaft portion 42 collectively by two-color molding, Insert molding may be performed in which molding is performed in a state in which the metered valve Vq, the introduction valve Vi, the discharge valve Vo, the valve portion Vc, the protruding portion 36, and the shaft portion 42 are stored.
　以上、添付図面を参照しながら本発明に係る好適な実施形態について説明したが、本発明は係る例に限定されないことは言うまでもない。上述した例において示した各構成部材の諸形状や組み合わせ等は一例であって、本発明の主旨から逸脱しない範囲において設計要求等に基づき種々変更可能である。 As described above, the preferred embodiments according to the present invention have been described with reference to the accompanying drawings, but it goes without saying that the present invention is not limited to such examples. Various shapes, combinations, and the like of the constituent members shown in the above-described examples are examples, and various modifications can be made based on design requirements and the like without departing from the gist of the present invention.
　例えば、上記実施形態では、バルブ部に流路１１を閉じる力を付与する付与部として、バルブ部Ｖｃに設けられた軸部４２を例示したが、この構成に限定されず、例えば、図９に示されるように、シート材４１とバルブ部Ｖｃとの間のバルブ保持孔３４に装填された粒状部材４２Ａであってもよい。粒状部材４２Ａとしては、図９に示す球状の他に円柱状、立方体状や直方体状であってもよい。粒状部材４２Ａとしては、投入後の姿勢に影響を受けることなくシート材４１とバルブ部Ｖｃとの距離を所定値に維持する観点から球状であることが好ましい。また、付与部がバルブ部Ｖｃと独立して設けられることにより、下面に接着層を有するシート材４１を上面６ｂから剥離する際に、シート材４１に付着した粒状部材４２Ａを一括的に容易に除去することが可能となる。 For example, in the above-described embodiment, the shaft portion 42 provided in the valve portion Vc is exemplified as the applying portion that applies the force for closing the flow path 11 to the valve portion. However, the present invention is not limited to this configuration. As shown, the granular member 42A loaded in the valve holding hole 34 between the seat material 41 and the valve portion Vc may be used. The granular member 42A may have a cylindrical shape, a cubic shape or a rectangular parallelepiped shape in addition to the spherical shape shown in FIG. The granular member 42A is preferably spherical from the viewpoint of maintaining the distance between the seat member 41 and the valve portion Vc at a predetermined value without being affected by the posture after being charged. Further, by providing the applying portion independently of the valve portion Vc, the granular member 42A attached to the sheet material 41 can be easily and collectively removed when the sheet material 41 having the adhesive layer on the lower surface is peeled off from the upper surface 6b. It can be removed.
　また、付与部としては、バルブ部Ｖｃに設けられる構成及びバルブ部Ｖｃ、シート材４１に設けられる構成の他に、図１０に示すように、シート材４１の下面から下方に突出して設けられた軸部４２Ｂとする構成であってもよい。この構成においても、シート材４１を剥した後に、上面６ｂから付与部が突出しないため、例えば、貫通孔３５ａに溶液を注入するための部材に付与部と干渉しないための凹部を形成する必要がなくなる。 In addition to the configuration provided in the valve portion Vc and the configuration provided in the valve portion Vc and the sheet material 41, the application portion is provided to protrude downward from the lower surface of the sheet material 41 as shown in FIG. The structure used as the axial part 42B may be sufficient. Even in this configuration, since the application portion does not protrude from the upper surface 6b after the sheet material 41 is peeled off, for example, a recess for preventing interference with the application portion needs to be formed in the member for injecting the solution into the through hole 35a. Disappear.
　また、上記実施形態では、バルブ部Ｖｃの一部がバルブ保持孔３４の内周面に付着する円筒状である構成を例示したが、この構成に限定されない。例えば、図１１に示すように、バルブ保持孔３４の内周面における上下方向の中途に、径方向内側に環状に突出する嵌合凸部３４ａを設け、バルブ部Ｖｃに嵌合凸部３４ａが嵌合する嵌合凹部４３を設ける構成であってもよい。この構成のバルブ部Ｖｃは、嵌合凸部３４ａに対して上下方向の両側から係合するため、シート材４１を貼り付けたときに、バルブ部Ｖｃが第１基材６から離脱ことを抑制できる。 In the above embodiment, a configuration in which a part of the valve portion Vc is a cylindrical shape attached to the inner peripheral surface of the valve holding hole 34 is illustrated, but the present invention is not limited to this configuration. For example, as shown in FIG. 11, a fitting convex portion 34 a that protrudes annularly radially inward is provided in the middle in the vertical direction on the inner peripheral surface of the valve holding hole 34, and the fitting convex portion 34 a is provided on the valve portion Vc. The structure which provides the fitting recessed part 43 to fit may be sufficient. Since the valve portion Vc having this configuration is engaged with the fitting convex portion 34a from both sides in the vertical direction, the valve portion Vc is prevented from being detached from the first base material 6 when the sheet material 41 is attached. it can.
　また、上記実施形態では、流路１１が第１基材６に形成される構成を例示したが、この構成に限定されず、第１基材６及び第２基材９の双方に跨って形成される構成、第２基材９に形成される構成であってもよい。図１２は、流路１１が形成された第２基材９の平面図である。第２基材９の上面９ｂには、バルブ部Ｖｃの突出部３６と対向する位置に、図１２に示されるように、窪み４０が形成されている。窪み４０は、流路１１の中途に配置されている。図１３は、図１２におけるＡ－Ａ線視断面図である。図１２には、突出部３６が窪み４０から離間して流路１１が開放された状態が示されている。図１２に示すように、窪み４０の最も低い（下側の）位置は、流路１１の底面よりも低い（下側の）位置である。流路１１の底面は、上面９ｂにおける窪み４０の稜線の位置から上側に傾斜している。 Moreover, in the said embodiment, although the structure in which the flow path 11 was formed in the 1st base material 6 was illustrated, it is not limited to this structure, It forms ranging over both the 1st base material 6 and the 2nd base material 9. The structure formed in the 2nd base material 9 may be sufficient. FIG. 12 is a plan view of the second base material 9 in which the flow path 11 is formed. A recess 40 is formed in the upper surface 9b of the second base material 9 at a position facing the protruding portion 36 of the valve portion Vc, as shown in FIG. The recess 40 is disposed in the middle of the flow path 11. 13 is a cross-sectional view taken along line AA in FIG. FIG. 12 shows a state in which the protruding portion 36 is separated from the recess 40 and the flow path 11 is opened. As shown in FIG. 12, the lowest (lower) position of the recess 40 is a lower (lower) position than the bottom surface of the flow path 11. The bottom surface of the channel 11 is inclined upward from the position of the ridge line of the recess 40 in the upper surface 9b.
　上記構成においては、バルブ部Ｖｃが下側に向かって弾性変形して突出部３６が窪み４０に当接することで流路１１を閉塞する。この構成では、突出部３６が窪み４０に当接する面積が大きくなるため、より確実に流路１１を閉塞することが可能になる。 In the above configuration, the valve portion Vc is elastically deformed downward, and the projecting portion 36 abuts on the recess 40 to close the flow path 11. In this configuration, since the area where the protruding portion 36 abuts on the recess 40 is increased, the flow path 11 can be more reliably closed.
　上記構成における、流路１１の深さとしては、０．０５ｍｍ以上、３ｍｍ以下が挙げられる（例えば、０．３ｍｍ）。軸部４２周囲の凹部の直径Ｄとしては、１ｍｍ以上、５ｍｍ以下が挙げられる（例えば、２．２ｍｍ）。軸部４２の直径としては、０．５ｍｍ以上、４ｍｍ以下が挙げられる（例えば、１．０ｍｍ）。上面６ｂからの軸部４２の突出量としては、０．０５ｍｍ以上、３ｍｍ以下が挙げられる（例えば、０．４ｍｍ）。バルブ部Ｖｃが流路１１を開放している際の突出部３６下端と窪み４０の下端との隙間量ｔとしては、０．０５ｍｍ以上、１．０ｍｍ以下が挙げられる（例えば、０．１ｍｍ）。上面９ｂにおける窪み４０の稜線で形成される円の直径としては、０．５ｍｍ以上、５．０ｍｍ以下が挙げられる（例えば、２．０ｍｍ）。窪み４０の球面の半径ＲＡとしては、１．０ｍｍ以上、５．０ｍｍ以下が挙げられる（例えば、１．４５ｍｍ）。突出部３６の球面の半径ＲＢとしては、１．０ｍｍ以上、５．０ｍｍ以下が挙げられる（例えば、１．８２ｍｍ）。 In the above configuration, the depth of the flow path 11 is 0.05 mm or more and 3 mm or less (for example, 0.3 mm). Examples of the diameter D of the recess around the shaft portion 42 include 1 mm or more and 5 mm or less (for example, 2.2 mm). Examples of the diameter of the shaft portion 42 include 0.5 mm or more and 4 mm or less (for example, 1.0 mm). Examples of the protruding amount of the shaft portion 42 from the upper surface 6b include 0.05 mm or more and 3 mm or less (for example, 0.4 mm). The gap amount t between the lower end of the protrusion 36 and the lower end of the recess 40 when the valve portion Vc opens the flow path 11 is 0.05 mm or more and 1.0 mm or less (for example, 0.1 mm). . Examples of the diameter of the circle formed by the ridge line of the recess 40 in the upper surface 9b include 0.5 mm or more and 5.0 mm or less (for example, 2.0 mm). Examples of the radius RA of the spherical surface of the recess 40 include 1.0 mm or more and 5.0 mm or less (for example, 1.45 mm). Examples of the radius RB of the spherical surface of the protrusion 36 include 1.0 mm or more and 5.0 mm or less (for example, 1.82 mm).
　また、上記実施形態では、バルブＶｃが突出部３６を有する構成を例示したが、この構成に限定されず、例えば、平板状のバルブＶｃが変形により窪み４０に当接して流路１１を閉塞する構成であってもよい。 Moreover, in the said embodiment, although the structure which the valve | bulb Vc has the protrusion part 36 was illustrated, it is not limited to this structure, For example, the flat valve | bulb Vc contact | abuts to the hollow 40 by a deformation | transformation, and the flow path 11 is obstruct | occluded. It may be a configuration.
　また、上記実施形態では、バルブ部Ｖｃがシート材４１の貼り付け及び剥離により流路１１を開閉する構成を例示したが、この構成に限定されない。
　例えば、上述した定量バルブＶｑ、導入バルブＶｉ、排出バルブＶｏと同様に、シート材４１の剥離後に、バルブ保持孔３４への駆動流体の供給及び供給停止により流路１１の開閉（溶液の流れ調整）を切り替える構成であってもよい。 Moreover, although the valve part Vc illustrated the structure which opens and closes the flow path 11 by sticking and peeling of the sheet | seat material 41 in the said embodiment, it is not limited to this structure.
For example, similarly to the quantitative valve Vq, the introduction valve Vi, and the discharge valve Vo described above, after the sheet material 41 is peeled off, the flow of the flow path 11 is controlled by supplying and stopping the supply of the driving fluid to the valve holding hole 34 (solution flow adjustment ) May be switched.
　また、上記実施形態では、バルブ部Ｖｃが流路１１を閉じたときに、付与部の上端が上面６ｂと面一の位置にある構成を例示したが、この構成に限定されず、貼り付けられたシート材４１が剥離しない範囲で付与部の上端が上面６ｂから突出している構成であってもよい。 Moreover, in the said embodiment, when the valve | bulb part Vc closed the flow path 11, the upper end of the provision part was illustrated in the same position as the upper surface 6b, However, It is not limited to this structure, Affixed The upper end of the applying portion may protrude from the upper surface 6b as long as the sheet material 41 does not peel off.
　また、上記実施形態では、シート材４１が接着層を有する構成を例示したが、この構成に限定されず、例えば、シート材を介して基材５を上下方向から挟持するクリップ等を用いてシート材を上面６ｂに貼り付ける構成であってもよい。 Moreover, in the said embodiment, although the structure which the sheet | seat material 41 has an adhesive layer was illustrated, it is not limited to this structure, For example, a sheet | seat is used using the clip etc. which clamp the base material 5 from the up-down direction via a sheet | seat material. The structure which affixes material on the upper surface 6b may be sufficient.
　１…流体デバイス、　５…基材、　６…第１基材、　６ａ…下面（接合面）、　６ｂ…上面（第１面）、　９…第２基材、　９ｂ…上面（接合面）、　１１…流路、　３４…バルブ保持孔（貫通孔）、　４１…シート材、　４２…軸部（付与部、駆動部）、　４２Ａ…粒状部材（付与部）、　６０…貼付装置、　６１…ローラー装置、　６２…袋体、　６３…吸引装置、　７１…載置部、　７２…剥離部、　７３…送液部、　７４…検出部、　Ｖ…バルブ装置、　Ｖｃ…バルブ部（弾性部材） DESCRIPTION OF SYMBOLS 1 ... Fluid device, 5 ... Base material, 6 ... 1st base material, 6a ... Lower surface (joining surface), 6b ... Upper surface (1st surface), 9 ... 2nd base material, 9b ... Upper surface (joining surface), 11 ... Flow path, 34 ... Valve holding hole (through hole), 41 ... Sheet material, 42 ... Shaft part (applying part, driving part), 42A ... Granular member (applying part), 60 ... Paste device, 61 ... Roller device, 62 ... bag body, 63 ... suction device, 71 ... mounting part, 72 ... peeling part, 73 ... liquid feeding part, 74 ... detection part, V ... valve device, Vc ... valve part (elastic member)

Claims (17)

  1. 　接合面で接合された第１基材及び第２基材を備え、
    　前記第１基材又は前記第２基材の少なくとも一方は、両基材を接合することにより流路を形成する溝を接合面に有し、
    　前記第１基材は、
    　前記流路と対向する位置に設けられる貫通孔と、
    　前記貫通孔の前記流路側の開口部を塞ぎ、変形により前記流路中の流体の流れを調整する弾性部材と、
    　前記第１基材の前記接合面とは逆側の第１面に、前記貫通孔の開口部を覆って剥離可能に貼り付けられたシート材と、
    　前記シート材と前記弾性部材との間に配置され、前記弾性部材を前記流路方向に変形させる駆動部と、
    を有するバルブを備える、流体デバイス。     A first base material and a second base material joined at the joining surface;
    At least one of the first base material or the second base material has a groove on the joint surface that forms a flow path by joining both base materials,
    The first substrate is
    A through hole provided at a position facing the flow path;
    An elastic member that closes the opening of the through hole on the flow path side and adjusts the flow of fluid in the flow path by deformation;
    A sheet material attached to the first surface opposite to the bonding surface of the first base material so as to cover the opening of the through-hole and to be peeled off;
    A drive unit that is disposed between the sheet material and the elastic member and deforms the elastic member in the flow path direction;
    A fluidic device comprising a valve having:
  2. 　前記バルブは、前記シート材を前記第１面から剥離した際に、前記弾性部材の変形が解消される、
    請求項１に記載の流体デバイス。     The valve is free from deformation of the elastic member when the sheet material is peeled from the first surface.
    The fluidic device according to claim 1.
  3. 　前記駆動部の前記貫通孔の貫通方向の長さは、前記シート材が前記第１面から剥離された状態においては前記第１面から突出し、前記シート材が前記第１面に貼り付けられた状態においては
     
    後に前記弾性部材を変形させて前記流路を閉じる長さである、
    　請求項１又は２に記載の流体デバイス。     The length of the through hole of the driving unit in the penetration direction protrudes from the first surface when the sheet material is peeled from the first surface, and the sheet material is attached to the first surface. In state
    It is a length to later deform the elastic member and close the flow path,
    The fluidic device according to claim 1 or 2.
  4. 　前記駆動部は、前記弾性部材と一体的に成形された成形体である、
    　請求項３に記載の流体デバイス。     The drive unit is a molded body molded integrally with the elastic member.
    The fluidic device according to claim 3.
  5. 　前記弾性部材は、前記第１基材と一体的に成形された成形体である、
    　請求項１～４のいずれか一項に記載の流体デバイス。     The elastic member is a molded body formed integrally with the first base material.
    The fluidic device according to any one of claims 1 to 4.
  6. 　前記弾性部材及び前記駆動部は、前記第１基材と異なる材料で形成されている、
    　請求項１～５のいずれか一項に記載の流体デバイス。     The elastic member and the drive unit are formed of a material different from that of the first base material.
    The fluidic device according to any one of claims 1 to 5.
  7. 　前記駆動部は、前記弾性部材に前記第１面側に突出して設けられた軸部である、
    　請求項１～６のいずれか一項に記載の流体デバイス。     The drive portion is a shaft portion provided on the elastic member so as to protrude toward the first surface side.
    The fluidic device according to any one of claims 1 to 6.
  8. 　前記駆動部は、前記シート材と前記弾性部材との間の前記貫通孔に装填された粒状部材である、
    　請求項１～３、５、６のいずれか一項に記載の流体デバイス。     The drive unit is a granular member loaded in the through hole between the sheet material and the elastic member.
    The fluidic device according to any one of claims 1 to 3, 5, and 6.
  9. 　前記シート材は、少なくとも前記粒状部材と当接する位置に接着層を有する、
    　請求項８に記載の流体デバイス。     The sheet material has an adhesive layer at least at a position in contact with the granular member.
    The fluidic device according to claim 8.
  10. 　前記駆動部は、前記シート材に前記貫通孔内に突出して設けられ、前記シート材が前記第１面に貼り付けられた状態においては前記弾性部材を変形させ、前記流路を閉じる長さを有する軸部である、
    　請求項１～３、５、６のいずれか一項に記載の流体デバイス。     The drive unit is provided in the sheet material so as to protrude into the through hole, and has a length that closes the flow path by deforming the elastic member in a state where the sheet material is attached to the first surface. A shaft part having
    The fluidic device according to any one of claims 1 to 3, 5, and 6.
  11. 　溶液が収容され、前記溶液を前記流路に供給するリザーバーをさらに備え、
    　前記バルブは、前記流路における前記リザーバーとの接続部に配置される、
    請求項１～１０のいずれか一項に記載の流体デバイス。     A reservoir that contains the solution and supplies the solution to the flow path;
    The valve is disposed at a connection portion with the reservoir in the flow path.
    The fluidic device according to any one of claims 1 to 10.
  12. 　前記第１基材は、前記バルブを複数備える、請求項１から１１のいずれか一項に記載の流体デバイス。 The fluid device according to any one of claims 1 to 11, wherein the first base material includes a plurality of the valves.
  13. 　前記シート材は、前記第１面側に、複数の前記貫通孔の開口部を覆って貼り付けられる、請求項１２に記載の流体デバイス。 The fluid device according to claim 12, wherein the sheet material is attached to the first surface side so as to cover the openings of the plurality of through holes.
  14. 　前記第１基材は、
    　前記流路と対向する位置に設けられる第２貫通孔と、
    前記第２貫通孔の前記流路側の開口部を塞ぎ、変形により前記流路中の流体の流れを調整する第２弾性部材と、を備える第２バルブを備え、
    　前記第２弾性部材は、前記貫通孔を流れる駆動流体の供給に応じて駆動され前記流路中の流体の流れを調整する、請求項１～１３のいずれか一項に記載の流体デバイス。     The first substrate is
    A second through hole provided at a position facing the flow path;
    A second valve comprising: a second elastic member that closes the flow path side opening of the second through hole and adjusts the flow of fluid in the flow path by deformation;
    The fluid device according to any one of claims 1 to 13, wherein the second elastic member is driven in response to supply of a driving fluid flowing through the through hole to adjust a flow of the fluid in the flow path.
  15. 　前記シート材は、前記流体デバイスを包装する袋体の一部である、請求項１～１４のいずれか一項に記載の流体デバイス。 The fluidic device according to any one of claims 1 to 14, wherein the sheet material is a part of a bag body for packaging the fluidic device.
  16. 　接合面で接合された第１基材及び第２基材の少なくとも一方に前記接合面に開口して形成された流路と対向する位置で前記第１基材を貫通する貫通孔と、
    　前記貫通孔の前記流路側に当該流路と対向して設けられ、弾性変形により前記流路中の流体の流れを調整するバルブ部と、
    　前記第１基材の前記接合面と逆側の第１面に剥離可能に貼り付けられ前記貫通孔を閉塞可能なシート材と、
    　前記貫通孔の前記シート材と前記バルブ部との間に配置され、前記シート材が前記第１面に貼り付けられたときに、前記バルブ部に前記流路を閉じる力を付与する付与部と、
    　を備えるバルブ装置。     A through-hole penetrating the first base material at a position opposed to a flow path formed by opening the joint surface in at least one of the first base material and the second base material joined at the joint surface;
    A valve portion provided on the channel side of the through hole so as to face the channel and adjusting the flow of fluid in the channel by elastic deformation;
    A sheet material that is detachably attached to the first surface of the first base material opposite to the joint surface, and can close the through hole;
    An applying portion that is disposed between the sheet material of the through-hole and the valve portion, and applies a force for closing the flow path to the valve portion when the sheet material is attached to the first surface; ,
    A valve device comprising:
  17. 　請求項１～１４のいずれか一項に記載の流体デバイスを載置する載置部と、
    　前記シート材を剥離する剥離部と、
    　前記流体デバイスの流路に対象物質を含む溶液を送液する送液部と、
    　前記溶液中の対象物質を検出する検出部と、を備える検出装置。     A mounting section for mounting the fluidic device according to any one of claims 1 to 14,
    A peeling portion for peeling the sheet material;
    A liquid feeding section for feeding a solution containing a target substance to the flow path of the fluid device;
    A detection unit that detects a target substance in the solution.
PCT/JP2018/016606 2018-04-24 2018-04-24 Fluid device, valve device, and detection device WO2019207644A1 (en)

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WO2018012429A1 (en) * 2016-07-13 2018-01-18 株式会社ニコン Fluid device, method of manufacturing fluid device, and valve for fluid device

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WO2016006615A1 (en) * 2014-07-07 2016-01-14 国立大学法人東京大学 Valve, fluid device, method for controlling fluid, and method for manufacturing valve
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