WO2019140722A1 - Use of butyryl glycine and derivative thereof for preparing animal feed additive - Google Patents

Abstract

Disclosed is the use of butyryl glycine and a derivative thereof in the preparation of an animal feed additive. Butyryl glycine is used in animal breeding experiments, and significantly improves the production performance of animals in terms of improvements in the weight gain rate of the average daily gain and the feed conversion rate. Butyryl glycine metal salts are used in animal breeding experiments, and compared to the butyryl glycine experiment group, have a similar efficacy for the production performance of animals. Farmers can feed animals with a butyryl glycine feed additive or a butyryl glycine derivative feed additive along with feeds, thus significantly improving the weight gain rate of the average daily gain and the feed conversion rate in postweaning pigs, and thus having broad application prospects.

Description

Application of Butyryl Glycine and Its Derivatives in Preparation of Animal Feed Additives Technical field:

The invention belongs to the field of animal feed additives, and particularly relates to the use of butyryl glycine and its derivatives in the preparation of animal feed additives and a feed composition comprising butyryl glycine or butyrylglycine derivatives.

Background technique:

Butyrylglycine is an acylated glycine that plays an important role in the regulation or degradation of acyl-CoA by-products in mammals and is an important metabolite for the diagnosis of inborn errors such as acyl-CoA deficient enzymes. It is used as a biomarker in the study of congenital metabolic disorders. Due to its suitable blood-brain barrier permeability and safety, butyryl glycine is used as a prophylactic or therapeutic agent for schizophrenia in the field of antipsychiatry. Butyryl glycine has a suitable cell membrane fat solubility, can promote the permeability of other substances in the cell membrane, and is used as a percutaneous absorption enhancer in the field of human skin care products. In the field of plant cultivation, butyryl glycine is also used as a plant protection agent or growth promoter.

Summary of the invention:

It is an object of the present invention to provide the use of butyrylglycine and its derivatives for the preparation of animal feed additives, and further to provide a feed composition comprising butyrylglycine or butyrylglycine derivatives.

In one aspect, the invention provides the use of a butyryl glycine or butyrylglycine derivative for the preparation of an animal feed additive.

The animal feed additive is an animal growth promoter.

In some embodiments, the compound used to prepare the animal feed additive is butyryl glycine.

The butyrylglycine has the structural formula shown in formula (I):

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is a butyryl glycine metal salt.

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is a butyryl glycine alkali metal salt.

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is preferably sodium butyryl glycinate or potassium butyryl glycinate.

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is a butyryl glycine alkaline earth metal salt.

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is preferably a butyryl glycinate calcium salt.

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is a butyryl glycine fatty ester.

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is preferably butyrylglycine ethyl ester.

The butyryl glycine ethyl ester has the structural formula shown in formula (II):

In some embodiments, the butyrylglycine derivative used to prepare the animal feed additive is preferably butyryl glycine butyl ester.

The butyryl glycine butyl ester has a structural formula as shown in formula (III):

In another aspect, the invention provides a feed composition comprising a butyrylglycine and/or butyrylglycine derivative.

In some embodiments, the feed composition comprises at least one of butyrylglycine and/or butyrylglycine derivatives and a feedable adjuvant.

In some embodiments, the feed composition further comprises an additional animal feed additive.

In some embodiments, the forage composition further comprises an animal feed material.

In some embodiments, the butyrylglycine derivative is a butyryl glycine metal salt.

In some embodiments, the butyrylglycine metal salt is a butyryl glycine alkali metal salt, and may preferably be a butyryl glycinate sodium ion salt or a butyryl glycine potassium ion salt.

In some embodiments, the butyrylglycine metal salt is a butyrylglycine alkaline earth metal salt, and may preferably be a butyryl glycine calcium ion salt.

In some embodiments, the butyrylglycine derivative is a butyrylglycine fatty ester, which may preferably be butyrylglycine ethyl ester or butyryl glycine butyl ester.

In another aspect, the invention provides the use of the above-described feed composition comprising butyrylglycine and/or butyrylglycine derivatives.

In some embodiments, the use of the forage composition in the preparation of an animal feed additive.

In some embodiments, the use of the feed composition in the preparation of an animal feed.

In another aspect, the invention also provides a method of improving the performance of a farmed animal.

In some embodiments, the method comprises administering to the farm animal a butyrylglycine and/or a butyrylglycine derivative in a manner admixed with the feed.

In some embodiments, the method comprises administering to a farm animal an ingestible composition comprising a butyrylglycine and/or butyrylglycine derivative provided by the present invention.

The beneficial effects of the invention:

Butyryl glycine was used in animal culture experiments, and the animal's production performance was significantly improved, in terms of the increase in the average daily weight gain rate and the feed conversion rate.

The butyrylglycine metal salt was used in animal culture experiments, and the effect on animal performance was similar to that of the butyrylglycine experimental group.

The feed composition of butyryl glycinate was used in animal culture experiments, with the chemical molar amount of butyrylglycine and its derivatives, compared with the butyrylglycine experimental group, butyrylglycerate has more significant animals. The improvement in production performance may be due to the better fat solubility of butyryl glycerate.

Any of the embodiments of any of the aspects of the invention may be combined with other embodiments as long as no contradiction occurs between them. Furthermore, in any of the embodiments of any of the aspects of the present invention, any of the technical features may be applied to the technical features in other embodiments as long as there is no contradiction between them.

The foregoing is merely an overview of some aspects of the invention, but is not limited thereto. The above content and other aspects will be described in more detail below.

A further detailed description of the invention.

The present invention is now described in detail with reference to the appended claims. In addition, some of the technical features of the present invention are clearly described in the various independent embodiments, but may be provided in combination in a single embodiment or in any suitable sub-combination.

Use of butyryl glycine and its derivatives.

Butyryl glycine and its derivatives are used in the preparation of animal feed additives.

The "feed additive" referred to in the present invention refers to a small amount or a trace amount of substances added during the processing, production and use of the feed ("Regulations on the Administration of Feed and Feed Additives", No. 609 of the State Council of the People's Republic of China).

Alternatively, the animal feed additive prepared using the butyrylglycine or butyrylglycine derivative is a livestock feed additive, a poultry feed additive, an aquaculture animal feed additive or a pet feed additive.

Specifically, the animal feed additive prepared using the butyrylglycine or butyrylglycine derivative is a livestock feed additive, and the livestock includes, but is not limited to, pigs, cows, sheep, horses, rabbits, donkeys, and the like at various stages of growth.

In one embodiment, the livestock is a weaned pig.

Specifically, animal feed additives prepared using butyrylglycine or butyrylglycine derivatives are poultry feed additives including, but not limited to, chickens, turkeys, ducks, geese, quails or pigeons at various stages of growth.

In one embodiment, the poultry is a broiler.

Specifically, the animal feed additive prepared using the butyrylglycine or butyrylglycine derivative is an aquaculture animal feed additive, including but not limited to fish, shrimp, crab, lynx, bullfrog or pupa at various stages of growth. .

In one embodiment, the aquaculture animal is grass carp.

Specifically, animal feed additives prepared with butyrylglycine or butyrylglycine derivatives are pet food additives, including but not limited to captive dogs or cats.

In some embodiments, applied to the preparation of animal feed additives is butyryl glycine.

In some embodiments, applied to the preparation of an animal feed additive is a butyryl glycine derivative.

Alternatively, the butyrylglycine derivative used in the preparation of the animal feed additive is a butyrylglycine metal ion salt produced by reacting a carboxylic acid group of butyrylglycine with a basic compound.

Specifically, the butyrylglycine metal salt is a butyrylglycine alkali metal ion salt, a butyrylglycine alkaline earth metal ion salt or other butyrylglycine metal ion salt.

In one embodiment, applied to the preparation of an animal feed additive is a butyryl glycinate sodium ion salt.

In one embodiment, applied to the preparation of an animal feed additive is a potassium butyryl glycinate salt.

In one embodiment, applied to the preparation of an animal feed additive is a butyryl glycinate calcium ion salt.

In one embodiment, applied to the preparation of an animal feed additive is a butyryl glycine copper ion salt.

In one embodiment, applied to the preparation of an animal feed additive is a butyrylglycine zinc ion salt.

Alternatively, the butyrylglycine derivative used in the preparation of the animal feed additive is a butyryl glycinate formed by reacting a carboxylic acid group of butyrylglycine with an alcohol.

The "alcohol" to which the present invention relates is a monohydric alcohol, and includes a linear or branched alkanol having 1 to 22 carbon atoms, and an alicyclic alcohol.

Specifically, the butyryl glycinate is a butyrylglycine linear alkyl ester, a butyryl glycine branched alkyl ester or a butyryl glycine alicyclic ester.

In one embodiment, the butyryl glycine derivative is butyryl glycine ethyl ester.

In some embodiments, the animal feed additive prepared with the butyryl glycine and or a derivative thereof is a premix, a composite premix, a liquid or a granule.

Feed composition.

The present invention provides a feed composition comprising butyrylglycine and or a derivative thereof.

The term "composition" as used in the present invention refers to a collective of compounds comprising one or more compounds which constitute an active ingredient.

The word "comprising" as used in the present invention is an open-ended expression, and includes the contents of the present invention, but does not exclude other aspects.

The "feedable auxiliary material" as used in the present invention refers to a substance which must be suitable for chemical or toxicology, is related to the composition of the feed or the farmed animal to be eaten, and is non-toxic to the animal.

In some embodiments, the feed composition comprises at least one of butyrylglycine and or butyrylglycine derivatives and a feedable adjuvant.

Optionally, the feed composition comprises a butyrylglycine derivative which is a butyrylglycine metal ion salt or a butyryl glycinate.

Specifically, the butyrylglycine metal ion salt includes, but is not limited to, butyrylglycine alkali metal ion salt or butyryl glycine alkaline earth metal ion salt.

Still more specifically, the butyrylglycine metal ion salt is a butyryl glycinate sodium ion salt, a butyryl glycine potassium ion salt or a butyryl glycinate calcium ion salt.

In one embodiment, the butyrylglycine metal ion salt is a butyryl glycinate sodium ion salt.

Specifically, the butyryl glycinate is a butyrylglycine linear alkyl ester, a butyryl glycine branched alkyl ester or a butyryl glycine alicyclic ester.

In one embodiment, the butyryl glycinate is butyryl glycine ethyl ester.

The feedable excipients include feed additives or carriers commonly used in feeds, binders, anti-caking agents, stabilizers, emulsifiers, diluents, adjuvants, vehicles, or combinations thereof.

The "carrier" of the present invention means a feedable substance capable of carrying an active ingredient, improving dispersibility, and having good chemical stability and adsorption, and is an organic carrier and an inorganic carrier. The organic carrier is a material containing a large amount of crude fiber, including but not limited to corn flour, corn cob powder, wheat bran, rice husk powder, defatted rice bran, taro, corn stalk powder, peanut shell powder and the like. The inorganic carrier is a mineral, mainly divided into calcium salts and silicon oxides, used for the preparation of trace element premixes, including but not limited to calcium carbonate, silicate, vermiculite, zeolite, sea blisters. Stone and so on.

The "diluent" referred to in the present invention refers to a substance in which the additive raw material is uniformly distributed in the material, and the high-concentration additive raw material is diluted into a low-concentration premix or premix, and the trace components can be separated from each other to reduce the active ingredient. The mutual reaction between them to increase the stability of the active ingredient without affecting the physicochemical properties of the relevant substances is an organic diluent and an inorganic diluent. Organic diluents include, but are not limited to, corn flour, deblasted corn flour, dextrose (glucose), sucrose, semolina with bran, fried soy flour, secondary flour, corn gluten meal, etc.; inorganic diluents include but not Limited to limestone, calcium dihydrogen phosphate, shell powder, kaolin (kaolin), salt and sodium sulfate.

The auxiliary agent is a wetting agent which induces the inherent viscosity of the substance, a binder which binds the substance, a disintegrating agent which breaks the whole sheet of the substance into a plurality of fine particles, and reduces the interparticles. A retention aid in Matcha or an anti-adhesive agent that prevents adhesion of materials, including but not limited to magnesium stearate, talc, vegetable oil, magnesium lauryl sulfate, starch, starch slurry, water, inorganic salts, dextrin, powdered sugar, etc. .

The term "solvent" as used in the present invention refers to a solvent required to dissolve or disperse a solid, including but not limited to water, ethanol, glycerin, and the like.

In some embodiments, the feed composition further comprises an additional animal feed additive and or animal feed material.

Optionally, the animal feed additive is a nutritive feed additive, a general nutritional feed additive or a pharmaceutical feed additive.

The nutritive feed additive refers to a small amount or trace substance added to the compound feed, balanced feed nutrients, improved feed utilization rate, and direct nutritional effects on the animal.

Specifically, the nutritional additive is an amino acid, an amino acid salt and the like, a vitamin and a vitamin, a mineral element and a chelating compound thereof, a microbial enzyme preparation or a non-protein nitrogen.

General feed additives, also called non-nutritive additives, are non-nutritive substances added to feeds to improve feed utilization, ensure feed quality and quality, and contribute to animal health or metabolism.

Specifically, the non-nutritive additives include growth promoters, insect repellent health supplements, flavoring and attractants, feed conditioners, feed conditioners, feed storage agents, and herbal additives.

Still more specifically, the non-nutritive additive is a growth promoter, including but not limited to butyric acid, calcium butyrate, sodium butyrate, tannic acid, thymol, thymol ester, thymol phenolate, 2-hydroxybenzoic acid, β-acid, β-ester, β-acid salt, hexahydro β-acid, hexahydro β-ester, hexahydro β-acid salt, benzoic acid or calcium benzoate.

In one embodiment, the non-nutritive additive is butyric acid having a synergistic effect with butyrylglycine or a derivative thereof.

Specifically, the pharmaceutical feed additive includes, but is not limited to, a veterinary drug premix having a disease preventing animal disease, promoting animal growth, and being incorporated into a feed for long-term use to incorporate a carrier or diluent.

Optionally, the animal feed materials are cereals and processed products thereof, oilseeds and processed products thereof, legume crop seeds and processed products, tubers, roots and processed products thereof, other seed and fruit products and processing thereof Products, forage, roughage and processed products, other plants, algae and processed products, dairy products and by-products, terrestrial animal products and their by-products, fish, other aquatic organisms and their by-products, minerals, microbial fermentation products and By-products, other feed ingredients and other feed materials.

Use of the feed composition.

The present invention relates to the use of the above feed composition comprising butyrylglycine and or a derivative thereof.

In some embodiments, the forage composition comprising butyrylglycine and or a derivative thereof is used in the preparation of an animal feed additive.

Alternatively, the animal feed additive prepared using the feed composition comprising butyrylglycine and or a derivative thereof is a livestock feed additive, a poultry feed additive, an aquaculture animal feed additive or a pet feed additive.

Specifically, a livestock feed additive is prepared using the above-described feed composition comprising butyrylglycine and a derivative thereof, including but not limited to pigs, cows, sheep, horses, rabbits, donkeys, etc. at various stages of growth. .

Specifically, poultry feed additives are prepared using the above-described feed composition comprising butyrylglycine and or a derivative thereof, including but not limited to chickens, ducks, geese, pigeons, and the like at various stages of growth.

Specifically, the aquaculture animal feed additive is prepared by using the above-described feed composition comprising butyrylglycine and a derivative thereof, including but not limited to fish, shrimp, crab, scorpion, scorpion in each growth stage. Wait.

Specifically, a pet food additive is prepared using the above-described feed composition comprising butyrylglycine and or a derivative thereof, including but not limited to a captive dog or cat.

In some embodiments, the animal feed additive prepared from the composition comprising butyrylglycine and or a derivative thereof is a premix, a composite premix, a liquid or a granule.

In some embodiments, the forage composition comprising butyrylglycine and or a derivative thereof is used in the preparation of an animal feed.

Alternatively, the animal feed prepared using the feed composition comprising butyrylglycine and or a derivative thereof is a livestock feed, a poultry feed, an aquaculture animal feed or a pet feed.

Specifically, the livestock feed is prepared using the above-described feed composition comprising butyrylglycine and a derivative thereof, including but not limited to pigs, cows, sheep, horses, rabbits, quails, and the like at various stages of growth.

Specifically, poultry feed is prepared using the above-described feed composition comprising butyrylglycine and or a derivative thereof, including but not limited to chickens, ducks, geese, pigeons, and the like at various stages of growth.

Specifically, the aquaculture animal feed is prepared by using the feed composition comprising butyrylglycine and a derivative thereof, including but not limited to fish, shrimp, crab, scorpion, scorpion, etc. at various growth stages. .

Specifically, the pet food is prepared using the above-described feed composition comprising butyrylglycine and or a derivative thereof, including but not limited to a captive dog or cat.

Alternatively, the feed prepared from the feed composition comprising butyrylglycine and or a derivative thereof is a concentrated feed, a compound feed, a composite premix or a concentrate supplement.

Specifically, the compound feed is a full-price compound feed.

A method to improve the performance of farmed animals.

In some feeding examples, the farmer feeds the butyric acid glycine feed additive or the butyryl glycine derivative feed additive to the animal for consumption, which can significantly improve the animal's performance.

Optionally, the feed additive is a premix, a composite premix, a granule or a water, and is mixed with the animal feed and then eaten by the animal.

Optionally, the animal is a domestic animal, a poultry, an aquaculture animal or a pet.

Specifically, the livestock includes, but is not limited to, pigs, cattle, sheep, horses, rabbits, donkeys, etc. at various stages of growth; the poultry includes, but is not limited to, chickens, ducks, geese, pigeons, etc. at various stages of growth; The aquaculture animals include, but are not limited to, fish, shrimp, crab, donkey, donkey, etc. at various stages of growth; the pets include, but are not limited to, captive dogs or cats.

In one embodiment, the farmer feeds the butyric acid glycine feed additive or the butyryl glycine derivative feed additive to the feed to the weaned pig, significantly increasing the weight gain rate and feed conversion rate of the average daily gain of the weaned pig.

In one embodiment, the farmer feeds the butyryl glycine feed additive or the butyryl glycine derivative feed additive to the broiler for consumption, significantly reducing the feed to meat ratio of the broiler to increase the feed conversion ratio.

In one embodiment, the farmer feeds the butyryl glycine feed additive or the butyryl glycine derivative feed additive to the fish for consumption.

In one embodiment, the farmer feeds the butyryl glycine feed additive or the butyryl glycine derivative feed additive to the feed to the puppies.

Alternatively, the butyryl glycine derivative feed additive fed to the animal for consumption is a butyryl glycine derivative metal ion salt feed additive or a butyric acid ester feed additive.

Specifically, the butyrylglycine derivative metal ion salt includes, but is not limited to, butyryl glycinate sodium ion salt, butyryl glycine potassium ion salt or butyryl glycine calcium ion salt; the butyryl glycinate is butyryl glycine Fatty ester.

In one embodiment, the butyryl glycine derivative feed additive is sodium butyrate glycinate, and the animal's feed intake is increased while the average daily gain gain rate is increased.

In one embodiment, the butyryl glycine derivative feed additive is butyryl glycine ethyl ester, and the performance of the animal is more remarkable than that of the butyryl glycine group.

In other feeding examples, the farmer feeds the feed composition comprising a butyrylglycine feed additive or a butyrylglycine derivative to the animal for significantly improved animal performance.

Optionally, the forage composition is a feed additive premix, a feed additive composite premix, a granule or a water agent, and is fed to the animal for consumption.

In one embodiment, the forage composition is a feed additive premix.

In one embodiment, the feed composition is a feed additive composite premix.

Optionally, the forage composition is a concentrated feed, a compound feed, a composite premix or a concentrate supplement, and is directly supplied to the animal as an animal diet.

In one embodiment, the forage composition is a full-price compound feed.

Detailed ways:

In order to clarify the objects, technical solutions and advantages of the present invention, the compounds, compositions and applications of the present invention are further described in detail below by way of examples. It is understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

Experimental Example A Application Effect of Butyryl Glycine and Its Derivatives in Broiler Materials

The experiment used a single factor randomized design to select 1200 Sanhuang feather broilers with an average body weight of 50g at the age of 1 day, and randomly divided into 10 treatment groups, each group of 6 replicates, half of each male and female, each repeat 20 Only three yellow feather broilers. Sterilize the house and utensils before the test. The test period is carried out under the same feeding and management conditions in the same chicken house. The basal diet is dominated by corn-soybean meal, and no additional antioxidants or growth promoters are added throughout the feeding process. Each test group was a blank group and one to nine groups. The blank group was given only to the basal diet, and the trial group 1-9 added 1000 ppm of butyrylglycine or butyrylglycine derivatives to the basal diet, as shown in Table 1. During the test period of 20 days, the test chickens were given free access to water and food, and fed twice a day. Weighed at 21 days of age (we stop feeding for 12 hours, no water), count the amount of chicken consumption, and calculate the average daily feed intake (ADFI, kg/d* only) of each group of test chickens. Average daily gain (ADG, kg/d* only) and feed to meat ratio (FCR).

Parameter statistics: During the test, weighed after 12 hours without stopping the water, and calculated the average daily feed intake (ADFI, kg/d* only) and average daily gain (ADG, kg/d* only) of each group. And meat to meat ratio (FCR). Calculated as follows:

Feed to meat ratio (FCR) = average daily feed intake / average daily gain.

The experimental data were statistically analyzed using SPSS18 software. The data were analyzed by one-way analysis of variance (ANOVA). If the difference between treatments was significant, Duncan's method was used for multiple comparisons. The significance level was 0.05. The test results are expressed as "mean ± standard error", and the test results are shown in Table 1.

From the results, it was found that butyric acid glycine and its derivatives had no effect on the feed intake of broilers compared with the blank control group, but it could increase the average daily gain of broilers and significantly reduce the feed to meat ratio of broilers.

Table 1 Grouping and test results of growth-promoting test of butyrylglycine and its derivatives on broilers

Example B Application of Butyryl Glycine and Its Derivatives in Pig Materials

600-day-old Du Changda ternary heterozygous piglets with a similar body weight were randomly divided into 10 treatment groups, with 3 replicates in each group, half male and half female, with 20 females in each group. The pigsty and utensils were sterilized before the test. The test period is housed in the same breeding and management conditions under the same pig pen. During the test, the test pigs were fed ad libitum and water, and fed twice a day. Each test group was a control group and trials 1 to 9 respectively. Among them, the control group was given only the basal diet, and the test groups 1 to 9 added 1500 ppm of butyrylglycine or butyrylglycine derivatives to the basal diet, as shown in Table 2. No additional antioxidants and growth promoters were added to the test groups throughout the feeding process. The test period was 28 days, weighed at 93 days in each repetition, and the feed intake of the test pigs was counted. The average daily feed intake (ADFI, kg/d* only) and average day of each group were calculated. Weight gain (ADG, kg/d* only) and feed to meat ratio (FCR).

Parameter statistics: During the test, weighed after 12 hours without stopping the water, and calculated the average daily feed intake (ADFI, kg/d* only) and average daily gain (ADG, kg/d* only) of each group. And meat to meat ratio (FCR). Calculated as follows:

Feed to meat ratio (FCR) = average daily feed intake / average daily gain.

The experimental data were statistically analyzed using SPSS18 software. The data were analyzed by one-way analysis of variance (ANOVA). If the difference between treatments was significant, Duncan's method was used for multiple comparisons, and the significance level was 0.05. The test results are expressed as "mean ± standard error", and the test results are shown in Table 2.

It can be seen from the results that butyrylglycine and its derivatives have no effect on the feed intake of the test piglets, but significantly increase the average daily gain of the test pigs and reduce the feed-to-meat ratio, and improve the conversion rate of the feed; The improvement results showed that sodium butyrate and potassium butyryl glycinate had similar effects as butyrylglycine to improve the performance of test piglets; the improvement of the ratio of meat to meat showed that butyrylglycine calcium salt, butyryl glycinate copper salt, butyryl glycine Magnesium salt, butyrylglycine zinc salt has similar effects as butyryl glycinate, and has a better effect on the feed conversion rate of test piglets than the butyrylglycine or butyrylglycine alkali metal salt.

Table 2 Effect of butyrylglycine and its derivatives on the performance of piglets

Example C Application of Butyryl Glycine and Its Derivatives in Aquatic Materials

(1) Test materials

Test fish: The test fish used in the year were healthy and lively, and the grass carp species with the same specifications were used for the formal culture test after being kept in a large cage (4×2×1.5 m ³ ) for 4 weeks. The experimental system was a floating small cage ( Specifications 1.1 × 1.1 × 1.1m ³ ), each small cage is equipped with an inflatable head, inflated 24 hours a day. Both the small cage and the holding cage are placed in a 3500 m ² pond at the test site. The pond has a water depth of about 1.5 m and the pond water is fully aerated. During the test, the 560 fishes that were hungry for 1 day were randomly divided into 7 groups, each group consisted of 4 replicates, and 20 fishes were placed in each repetition. They were weighed in each repeat and placed in 36 cages and fed separately. Feed different test feeds.

Test feed: The test feed was prepared according to the formula of Table 3. Different test groups were added with the same dose of different butyrylglycine or its derivative according to Table 4. The feed ingredients used were finely pulverized and then made into a floating bulking material with a particle size of 3 mm through a Jiangsu shepherd expansion unit. The mold temperature was 130 ° C, and 3% soybean oil was sprayed through the spray equipment, and sealed in a cool place for storage.

Table 3 Formulated grass carp feed formula and chemical composition (%wt.)

Raw material composition	content(%)	Raw material composition	content(%)
Fish meal	9.0	Soybean oil	3.0
Casing powder	3.0	Phospholipid	9.0
Cardamom	12.0	Gluten	4.0
Dish	12.0	Blood cell powder	2.0
Monosodium glutamate	3.0	Vc-phosphate	0.1

Secondary powder	12.6	Calcium dihydrogen phosphate	1.8
flour	17.0	Choline chloride	0.2
Bentonite	0.70	Multidimensional	0.1
Rice bran	10.0	Micro-mineral premix	0.5

Table 4 Grouping of growth-promoting tests of butyryl glycine and its derivatives

Group	testing sample	Dose (ppm)
Blank control group	-	-
Test 1	Butyryl glycine	840
Test 2	Butyryl glycinate sodium salt	840
Test 3	Butyryl glycine calcium salt	840
Test 4	Butyryl glycine copper salt	840
Test 5	Butyryl glycine zinc salt	840
Test 6	Butyryl glycine ethyl ester	840

(2) Test method

Trial management: The test was fed by artificial food restriction. The feeding amount was adjusted once a week. The feeding level of each group (according to the initial weight) was completely the same. It was fed twice a day (7:30 and 15:00), and the total feeding amount was The test group was 500 g/repeat. The trial lasted for 8 weeks. The water quality was monitored regularly during the test. The whole process water temperature was 26.88±3.08°C, DO>5.0mg O L ^-1 , pH 7.8, ammonia nitrogen <0.50mg N L ^-1 , nitrite nitrogen <0.05mg N L ^-1 .

Parameter statistics: During the test, the whole cage fish were weighed after 1 d of feeding, and the weight gain rate (WG, %), feed coefficient (FCR) and survival rate (SR, %) were calculated. Calculated as follows:

Weight gain rate (WG, %) = 100 × (average final weight - average initial weight) / average initial weight;

Feed coefficient (FCR) = food intake / fish weight gain;

Survival rate (SR, %) = 100 × number of fish at the end of the test / number of fish at the start of the test.

(3) Test results

From the test results shown in Table 5, it was found that the application of butyryl glycine and its derivatives in the aquaculture material can significantly reduce the feed ratio of the grass carp to increase the conversion rate of the feed, although it can increase the average weight gain rate of the grass carp but is not significant.

Table 5 Test results of application of butyryl glycine and its derivatives in aquatic materials

	Average initial weight (g)	Average dead weight (g)	Weight gain rate (%)	(FCR)	SR (%)
Blank control group	412.25±15.56	938.50±22.81	128.01±3.32	1.598±0.025 a	100
Test 1	403.00±11.50	975.66±19.66	142.28±2.14	1.468±0.022 b	100
Test 2	414.75±9.97	988.25±11.35	138.52±3.45	1.462±0.013 b	100
Test 3	409.58±10.63	989.34±11.08	141.92±5.19	1.450±0.024 b	100
Test 4	411.50±9.08	998.50±5.50	142.91±3.92	1.432±0.009 b	100
Test 5	415.25±8.87	990.25±10.66	138.64±2.68	1.460±0.009 b	100
Test 6	414.46±7.32	992.70±3.79	139.74±4.28	1.452±0.020 b	100

The above-described embodiments are merely illustrative of several embodiments of the invention, and other ways of implementing the invention. Accordingly, the embodiments of the present invention are to be construed as illustrative and not restrictive of the scope of the invention, Equivalent content added in .

Claims (10)

1. Use of butyryl glycine or butyrylglycine derivatives in the preparation of animal feed additives.
2. The use according to claim 1, wherein the butyrylglycine derivative is a butyryl glycine metal salt.
3. The use according to claim 2, wherein the butyryl glycine metal salt is a butyryl glycinate sodium ion salt or a butyryl glycine potassium ion salt.
4. The use according to claim 2, wherein the butyryl glycine metal salt is a butyryl glycinate calcium ion salt.
5. The use according to claim 1, wherein the butyrylglycine derivative is a butyryl glycine fatty ester.
6. The use according to Claim 5, characterized in that the butyrylglycine derivative is butyryl glycine ethyl ester or butyryl glycine butyl ester.
7. A feed composition characterized in that the composition comprises a butyrylglycine or a butyrylglycine derivative.
8. The feed composition according to claim 7, wherein the butyrylglycine derivative is a butyryl glycine metal salt or a butyryl glycine fatty acid, and the butyryl glycine metal salt is sodium butyrate glycinate. Salt, butyryl glycine potassium salt or butyryl glycine calcium salt, the butyryl glycine fatty ester is butyryl glycine ethyl ester or butyryl glycine butyl ester.
9. A feed composition according to claim 7 wherein said forage composition comprises an additional animal feed additive.
10. A feed composition according to any one of claims 7-9, wherein the forage composition comprises an animal feed material.