WO2019131924A1 - Corps moulé et procédé de production de corps moulé - Google Patents

Corps moulé et procédé de production de corps moulé Download PDF

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Publication number
WO2019131924A1
WO2019131924A1 PCT/JP2018/048286 JP2018048286W WO2019131924A1 WO 2019131924 A1 WO2019131924 A1 WO 2019131924A1 JP 2018048286 W JP2018048286 W JP 2018048286W WO 2019131924 A1 WO2019131924 A1 WO 2019131924A1
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WO
WIPO (PCT)
Prior art keywords
amino acid
polypeptide
acid sequence
seq
composition
Prior art date
Application number
PCT/JP2018/048286
Other languages
English (en)
Japanese (ja)
Inventor
アリナ クダシェワ
Original Assignee
Spiber株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber株式会社 filed Critical Spiber株式会社
Publication of WO2019131924A1 publication Critical patent/WO2019131924A1/fr

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    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08KUse of inorganic or non-macromolecular organic substances as compounding ingredients
    • C08K3/00Use of inorganic substances as compounding ingredients
    • C08K3/02Elements
    • C08K3/04Carbon
    • CCHEMISTRY; METALLURGY
    • C08ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08LCOMPOSITIONS OF MACROMOLECULAR COMPOUNDS
    • C08L89/00Compositions of proteins; Compositions of derivatives thereof

Definitions

  • the mixing step may be a step of mixing the polypeptide powder and the carbon powder, wherein the composition may be a mixed powder including the polypeptide powder and the carbon powder.
  • epitope tags utilizing antigen-antibody reactions can also be used.
  • a peptide (epitope) showing antigenicity as a tag sequence an antibody against the epitope can be bound.
  • the epitope tag include HA (peptide sequence of hemagglutinin of influenza virus) tag, myc tag, FLAG tag and the like.
  • the type of expression vector can be appropriately selected according to the type of host, such as a plasmid vector, a virus vector, a cosmid vector, a fosmid vector, an artificial chromosome vector and the like.
  • a vector capable of autonomous replication in a host cell or capable of being integrated into the host chromosome and containing a promoter at a position capable of transcribing a gene encoding a target recombinant polypeptide is preferable. Used for
  • Eukaryotic hosts can include, for example, yeast and filamentous fungi (molds and the like).
  • the amount of carbon black blended in the composition may be, for example, 0.05 parts by mass or more, preferably 0.5 parts by mass or more, and more preferably 1.0 parts by mass with respect to 100 parts by mass of the polypeptide. It is above.
  • the amount of carbon black blended in the composition may be, for example, 20 parts by mass or less, preferably 10 parts by mass or less, more preferably 5 parts by mass or less, with respect to 100 parts by mass of the polypeptide.
  • the method for producing a molded article includes a mixing step of mixing a polypeptide and carbon black to obtain a composition, and a molding step of introducing the composition into a mold and molding the same. .
  • the polypeptide to be mixed in the mixing step may have a powdery (lyophilized powder etc.) or fibrous (spinned etc.) shape, and the molded body has a composition containing the polypeptide of such shape It may be a fused body of matter.
  • FIG. 1 is a schematic cross-sectional view showing a pressure molding machine that can be used to manufacture a molded body.
  • the press molding machine 10 shown in FIG. 1 is provided with a heatable mold 2 in which a through hole is formed, and an upper pin 4 and a lower pin 6 capable of moving up and down in the through hole of the mold 2. It is.
  • the above composition is introduced into the space created by inserting the upper pin 4 or the lower pin 6 into the mold 2 and the composition is heated by the upper pin 4 and the lower pin 6 while the mold 2 is heated. By compacting, a molded article can be obtained.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Organic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne : un corps moulé d'une composition contenant un polypeptide et du noir de carbone ; et un procédé de production de corps moulé comprenant une étape de mélange consistant à mélanger un polypeptide et du noir de carbone pour obtenir une composition, et une étape de moulage consistant à mouler la composition par chauffage et mise sous pression de la composition.
PCT/JP2018/048286 2017-12-27 2018-12-27 Corps moulé et procédé de production de corps moulé WO2019131924A1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017-251738 2017-12-27
JP2017251738A JP2021063143A (ja) 2017-12-27 2017-12-27 モールド成形体及びモールド成形体の製造方法

Publications (1)

Publication Number Publication Date
WO2019131924A1 true WO2019131924A1 (fr) 2019-07-04

Family

ID=67063825

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2018/048286 WO2019131924A1 (fr) 2017-12-27 2018-12-27 Corps moulé et procédé de production de corps moulé

Country Status (2)

Country Link
JP (1) JP2021063143A (fr)
WO (1) WO2019131924A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001517253A (ja) * 1995-11-29 2001-10-02 ミッドウエスト グレイン プロダクツ,アイエヌシー. 穀物タンパク質を主成分とする生分解性固形製品及びその成形方法
WO2014002605A1 (fr) * 2012-06-28 2014-01-03 スパイバー株式会社 Fibre de protéine teintée dans la masse et procédé pour produire celle-ci
WO2017047504A1 (fr) * 2015-09-18 2017-03-23 Spiber株式会社 Article moulé et procédé de fabrication d'article moulé
WO2017047503A1 (fr) * 2015-09-18 2017-03-23 Spiber株式会社 Article moulé et son procédé de fabrication

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001517253A (ja) * 1995-11-29 2001-10-02 ミッドウエスト グレイン プロダクツ,アイエヌシー. 穀物タンパク質を主成分とする生分解性固形製品及びその成形方法
WO2014002605A1 (fr) * 2012-06-28 2014-01-03 スパイバー株式会社 Fibre de protéine teintée dans la masse et procédé pour produire celle-ci
WO2017047504A1 (fr) * 2015-09-18 2017-03-23 Spiber株式会社 Article moulé et procédé de fabrication d'article moulé
WO2017047503A1 (fr) * 2015-09-18 2017-03-23 Spiber株式会社 Article moulé et son procédé de fabrication

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LEI JONG: "Natural Rubber Protein as Interfacial Enhancement for Bio-Based Nano-Fillers", JOURNAL OF APPLIED POLYMER SCIENCE, vol. 130, no. 3, 2013, pages 2188 - 2197 *

Also Published As

Publication number Publication date
JP2021063143A (ja) 2021-04-22

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