WO2019093832A3 - Novel floury endosperm gene, molecular marker, and use thereof - Google Patents

Novel floury endosperm gene, molecular marker, and use thereof Download PDF

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Publication number
WO2019093832A3
WO2019093832A3 PCT/KR2018/013661 KR2018013661W WO2019093832A3 WO 2019093832 A3 WO2019093832 A3 WO 2019093832A3 KR 2018013661 W KR2018013661 W KR 2018013661W WO 2019093832 A3 WO2019093832 A3 WO 2019093832A3
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WIPO (PCT)
Prior art keywords
present
floury endosperm
rice
novel
molecular marker
Prior art date
Application number
PCT/KR2018/013661
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French (fr)
Korean (ko)
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WO2019093832A2 (en
Inventor
정지웅
권순욱
장성규
이아림
Original Assignee
대한민국(농촌진흥청장)
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Application filed by 대한민국(농촌진흥청장) filed Critical 대한민국(농촌진흥청장)
Priority to JP2020525962A priority Critical patent/JP7004816B2/en
Priority to CN201880077492.0A priority patent/CN111630188A/en
Publication of WO2019093832A2 publication Critical patent/WO2019093832A2/en
Publication of WO2019093832A3 publication Critical patent/WO2019093832A3/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The present invention relates to a marker for identifying characteristics of floury endosperm and a use thereof. According to the present invention, primer sets targeting single-nucleotide polymorphism (SNP) sites identified in the present invention can be used to determine all rice cultivars, specifically, the floury endosperm of a Japonica rice line simply and accurately at the early stage of breeding. Specifically, the evaluation of floury endosperm of rice cultivars can be applied to the selection of early-stage inbred lines or individual F2 plants. Furthermore, the present invention can be expanded and applied to processed products having rice as a main material.
PCT/KR2018/013661 2017-11-10 2018-11-09 Novel floury endosperm gene, molecular marker, and use thereof WO2019093832A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2020525962A JP7004816B2 (en) 2017-11-10 2018-11-09 New powdered endosperm genes, molecular markers and their uses
CN201880077492.0A CN111630188A (en) 2017-11-10 2018-11-09 Novel floury endosperm gene, molecular marker and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0149236 2017-11-10
KR1020170149236A KR102010859B1 (en) 2017-11-10 2017-11-10 Markers for identifying floury endosperm characteristics and use thereof

Publications (2)

Publication Number Publication Date
WO2019093832A2 WO2019093832A2 (en) 2019-05-16
WO2019093832A3 true WO2019093832A3 (en) 2019-07-18

Family

ID=66438942

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/013661 WO2019093832A2 (en) 2017-11-10 2018-11-09 Novel floury endosperm gene, molecular marker, and use thereof

Country Status (4)

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JP (1) JP7004816B2 (en)
KR (1) KR102010859B1 (en)
CN (1) CN111630188A (en)
WO (1) WO2019093832A2 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110747288A (en) * 2019-11-14 2020-02-04 扬州大学 Rice large grain gene function marker and application
CN110616277A (en) * 2019-11-14 2019-12-27 扬州大学 Rice grain length gene function marker and application thereof
CN110894542A (en) * 2019-12-31 2020-03-20 扬州大学 Primer for identifying types of GS5 gene and GLW7 gene of rice and application of primer
KR102584678B1 (en) * 2020-12-22 2023-10-06 대한민국 ‘Samkwang(SA)-flo3’, floury endosperm rice mutant derived from ‘Samkwang’
WO2023049708A1 (en) * 2021-09-22 2023-03-30 Herbalife International Of America, Inc. Methods and compositions for identifying botanical material using arms-pcr

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110092142A (en) * 2010-02-08 2011-08-17 대한민국(농촌진흥청장) A floury japonica rice line, namil(sa)-flo2, suitable for dry milling process and the food compositions containing namil(sa)-flo2 as an active ingredient
KR20110109063A (en) * 2010-03-30 2011-10-06 서울대학교산학협력단 Floury endosperm gene, flo(a), molecular marker and fine mapping of flo(a) in rice

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101381415A (en) * 2000-11-09 2009-03-11 联邦科技产业研究组织 Barley with reduced ssii activity and starch containing products with a reduced amylopectin content
CN103725768A (en) * 2012-10-15 2014-04-16 丁筱玲 Research method for rice genomics
CN103333953B (en) * 2013-05-23 2015-03-25 浙江大学 Method for identification of purity of two-line hybrid rice seed
CN104561315A (en) * 2015-01-09 2015-04-29 上海市农业科学院 SNP (Single Nucleotide Polymorphism) detection method of rice high-resistant starch content mutant gene sbe3-rs

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20110092142A (en) * 2010-02-08 2011-08-17 대한민국(농촌진흥청장) A floury japonica rice line, namil(sa)-flo2, suitable for dry milling process and the food compositions containing namil(sa)-flo2 as an active ingredient
KR20110109063A (en) * 2010-03-30 2011-10-06 서울대학교산학협력단 Floury endosperm gene, flo(a), molecular marker and fine mapping of flo(a) in rice

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KANG, H. G. ET AL.: "White-core endosperm floury endosperm-4 in rice is generated by knockout mutations in the C4-type pyruvate orthophosphate dikinase gene (OsPPDKB", THE PLANT JOURNAL, vol. 42, no. 6, 2005, pages 901 - 911, XP009113978 *
MO, Y. J. ET AL.: "Agronomic and genetic analysis of Suweon 542, a rice floury mutant line suitable for dry milling Rice", vol. 6, no. 37, 2013, pages 1 - 12, XP055625053 *
MO, YOUNG-JUN ET AL.: "Genetic Analysis on Floury Endosperm Characteristics of 'Namil(SA)-flo l', a Japonica Rice Mutant Line", KOREAN JOURNAL OF CROP SCIENCE, vol. 58, no. 3, 2013, pages 283 - 291, XP055625057 *

Also Published As

Publication number Publication date
JP2021502096A (en) 2021-01-28
WO2019093832A2 (en) 2019-05-16
KR102010859B1 (en) 2019-08-16
CN111630188A (en) 2020-09-04
JP7004816B2 (en) 2022-02-04
KR20190053406A (en) 2019-05-20

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