WO2019087972A1 - Procédé d'inhibition de maladie de conservation des fruits mettant en œuvre un acide peracétique - Google Patents

Procédé d'inhibition de maladie de conservation des fruits mettant en œuvre un acide peracétique Download PDF

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Publication number
WO2019087972A1
WO2019087972A1 PCT/JP2018/039923 JP2018039923W WO2019087972A1 WO 2019087972 A1 WO2019087972 A1 WO 2019087972A1 JP 2018039923 W JP2018039923 W JP 2018039923W WO 2019087972 A1 WO2019087972 A1 WO 2019087972A1
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Prior art keywords
fruit
peracetic acid
storage
fruits
ppm
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PCT/JP2018/039923
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English (en)
Japanese (ja)
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光弘 加藤
智津子 影山
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保土谷化学工業株式会社
静岡県
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Application filed by 保土谷化学工業株式会社, 静岡県 filed Critical 保土谷化学工業株式会社
Priority to JP2019550328A priority Critical patent/JP7321434B2/ja
Priority to CN201880065624.8A priority patent/CN111200936A/zh
Publication of WO2019087972A1 publication Critical patent/WO2019087972A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing the group; Thio analogues thereof

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  • the present invention relates to a method for controlling storage diseases of fruits using peracetic acid. Specifically, a method for controlling storage diseases of harvested fruits, which comprises treating the entire surface of fruits and their bellflowers with a fungicide containing a specific concentration of peracetic acid prior to their storage and / or transportation. It relates to a method characterized in that it comprises the step of contacting.
  • storage disease which occurs from the time of harvesting agricultural products to storage, transportation, and sale is a problem because it perishes fruits, particularly fresh foods such as fruits and vegetables.
  • Storage diseases may be caused by pathogens that are infected before harvest, or may be caused by pathogens that are newly transmitted from wounds generated during various operations after harvest.
  • black mold disease Anthrax, gray mold, blue mold, green mold and the like are known.
  • These storage diseases are also known to be derived from various filamentous fungi (generally referred to as mold) such as Penicillium (genus) and Botrytis (botrytis).
  • peracetic acid preparations have been approved as food additives. It is known that peracetic acid can be applied by irrigation treatment as a fungicide for agriculture and horticulture, for example, as a fungicide for diseases of rice, tomato, Chinese cabbage and cabbage (see, for example, Patent Document 1).
  • An object of the present invention is to provide a method for controlling storage diseases of fruits without using pesticides.
  • the present inventors focused on peracetic acid preparations which have recently been approved as food additives in Japan, and have been approved for use in various countries of the world, and as a result, fruits harvested with extremely low concentrations of peracetic acid
  • the present invention has been completed by finding that the storage diseases can be suppressed during storage and / or transportation periods ranging from several days to several weeks required for domestic remote location or overseas supply.
  • the present invention is as follows: [1] A process comprising contacting the harvested fruit with its bactericidal agent containing peracetic acid at a concentration of 10 ppm or more and less than 100 ppm, prior to storage and / or transportation of the fruit, Method of controlling storage diseases of fruits using peracetic acid.
  • [5] The method for controlling fruit storage diseases using peracetic acid according to any one of the above [1] to [4], which does not include a washing step of a bactericide after the contacting step.
  • [6] The method for controlling fruit storage diseases using peracetic acid according to any one of the above [1] to [5], which comprises a drying step after the contacting step.
  • [7] The method for controlling the storage disease of fruit according to any of the above [1] to [6], wherein the harvested fruit is a fruit of citrus fruits.
  • [8] The method of suppressing storage disease of fruit according to any one of the above [1] to [6], wherein the harvested fruit is a fruit of a solanaceous plant.
  • the method of the present invention is an inexpensive and safe method applicable to harvested fruits, since it uses peracetic acid as a food additive at very low concentrations as a food additive.
  • the sterilization method of the present invention can be easily carried out by immersing the fruit with the bactericidal agent together with the buccal portion or spraying the bactericide together with the buccal portion onto the fruit and the subsequent sterilization Since the step of washing the agent is unnecessary, it can be easily carried out by a simple operation.
  • the method of the present invention is excellent in that the bactericidal activity of the filamentous fungi is high and storage diseases can be suppressed during storage and / or transportation over several days to several weeks required for domestic remote location or overseas supply. It is.
  • fruits The term “harvested fruits” to which the present invention is applied means harvested fruits and vegetables, and is particularly limited as long as the fruits and vegetables are concerned with storage diseases during storage and / or transportation. I will not.
  • fruits include the fruits of Rosaceae plants such as apples and pears, the fruits of Citrusaceae plants such as mandarin oranges, mandarin oranges, lemons and kumquats, and preferred examples include mandarin oranges (satsuma mandarin oranges), mandarin oranges, lemons, Examples include citrus fruits of the citrus family such as kumquat.
  • fruits of cucurbitaceous plants such as watermelon, bitter gourd, tougan, pumpkin and melon
  • fruits of solanaceous plants such as tomato, eggplant and green peppers
  • preferred examples include tomato, eggplant and sweet peppers Fruits of the solanaceous plants of
  • the term "fruiting branch” generally refers to the part separated and branched from branches and stems and having a fruit attached to the end, but the term “fruiting stem” referred to in the present invention means Refers to the part that is cut off near the fruit and that contains a haze or a haze.
  • the "bactericidal agent" used in the method of the present invention is an aqueous solution containing a predetermined concentration of peracetic acid.
  • the concentration of peracetic acid is 10 ppm or more and less than 100 ppm, and the lower limit thereof is preferably 20 ppm or more, more preferably 25 ppm or more, and the upper limit thereof is preferably 90 ppm or less, more preferably 80 ppm or less, particularly preferably 50 ppm or less.
  • peracetic acid has the following formula It is in equilibrium as shown by.
  • the concentration of peracetic acid in the present invention means the concentration of total peroxide converted to peracetic acid.
  • a commercially available peracetic acid preparation may be used as a microbicide in the present invention, and a commercially available peracetic acid preparation may be used after diluting it to a predetermined concentration.
  • commercially available peracetic acid preparations optionally include 1-hydroxyethylidene-1,1-diphosphonic acid (alias: etidronic acid; HEDP) and octanoic acid (in that case) Containing by-produced peroctanoic acid).
  • Storage disease refers to a disease that occurs from the harvest of agricultural products to storage, transportation, and sale, and so-called “market disease” occurring in the market, “storage disease” that occurs during storage. And “imported diseases” that occur during transportation of imported agricultural products.
  • blue mold Pestial Fungus: Penicillium italicum derived from Penicillium (genus), Botrytis (Botrytis), Geotrichum, etc.
  • green mold genus (gener of pathogen)
  • Examples include, but are not limited to, Penicillium digitatum), Botrytis cinerea (Bacterial organism: Botrytis cinerea), Botrytis cinerea (Bacteriostatic bacterium: Geotrichum candidum), and the like.
  • Typical examples of storage diseases which can be suppressed by the method of the present invention are Fusarium head blight, green mold and / or Botrytis cinerea derived from filamentous fungi such as Penicillium or Botrytis.
  • Botrytis cinerea is an airborne disease that, when harvested, when stored fruit is infected, it produces gray mold and rot progresses.
  • the fungus of Botrytis cinerea germinates under low temperature, dark, high humidity conditions and the infection spreads by hyphal contact transmission, so the fruits after harvest contain infected fruits or after harvest Infections spread during storage if newly infected with a pathogen from wounds created during various tasks.
  • Fruits treated with the storage disease control method of the present invention can suppress the storage disease over storage and / or transportation periods of several days to several weeks.
  • “preventing storage disease” means a test treated by the method of the present invention in comparison with the test fruit of the untreated group or the control drug group as shown in the examples described later. It means that the number of rotten fruits and the rotten area ratio in rot fruits decrease.
  • the storage disease control method of the present invention comprises the step of contacting the harvested fruit with a bactericidal agent containing peracetic acid at a concentration of 10 ppm or more and less than 100 ppm, prior to storage and / or transportation of the fruit. It is characterized by including.
  • the contacting step is not particularly limited as long as the bactericidal agent is sufficiently applied to the surface of the fruit, but typically, the harvested fruit is immersed in the bactericidal agent together with the bellflower, or It is carried out by spraying a bactericide on the harvested fruits, together with the bellflower part.
  • the soaking time varies depending on the type of fruit used, the place of production, the peracetic acid concentration in the microbicide and the temperature, etc., but is usually in the range of 5 seconds to 5 minutes.
  • the immersion is usually carried out under a temperature environment of 0 to 40 ° C., preferably at normal temperature (atmospheric temperature; eg 25 ° C. ⁇ 10 ° C.).
  • atmospheric temperature eg 25 ° C. ⁇ 10 ° C.
  • the bellflower has a complicated uneven shape, which is called “gaku” or “huta”, as compared with the whole surface of the fruit, and because the bellflower is cut off near the fruit during harvesting. Because it is short and dries and hardens with the passage of time after harvest as compared to the surface of the fruit, if the contact of the bactericidal agent with the stem portion is insufficient, the bellflower will contact with the surface of the fruit due to vibration or shock during storage. When the fruit surface is damaged, pathogens that have survived in the bellflower part are infected on the fruit surface, and as a result, the fruit is rotted. Therefore, sufficient contact of the fungicide not only on the fruit surface but also on the bellflower part is important.
  • the fruit may be separated from the bactericide and the bactericide may be washed with running or stored water, but the bactericide (peracetic acid) used in the method of the present invention will transpiration or decompose spontaneously Because the final decomposition product (water, oxygen, acetic acid) is non-toxic, the washing step of the germicide may be omitted.
  • the bactericide peracetic acid
  • the fruits subjected to the contacting step and optionally the washing step may be further subjected to a drying step. Drying is usually carried out at a temperature of 0-40 ° C., in the range of 1-24 hours, preferably air-drying (ie natural drying) at ambient temperature.
  • Test Example 1 Test target bacteria on agar medium : blue mold (Penicillium italicum) and green mold (Penicillium digitatum) Preparation of culture medium: After cooling a sterilized potato dextrose agar (PDA) culture medium to about 45 ° C., each drug (peracetic acid, calcium hypochlorite, sodium hypochlorite) has a predetermined concentration (10, 25, 50) , 100, 200 ppm) were added to prepare a test medium.
  • PDA potato dextrose agar
  • Method A spore suspension of Fusarium head blight fungus and Fusarium head blight fungus was streaked on PDA medium, and after overnight culture, monospores were transferred to assay medium (three replicates in each section).
  • Test target bacteria in liquid medium blue mold (Penicillium italicum) and green mold (Penicillium digitatum)
  • Preparation of culture medium Dispense 5 mL of potato dextrose (PD) liquid into sterile test tubes, and each drug (peracetic acid, calcium hypochlorite, sodium hypochlorite) has a predetermined concentration (10, 25, 50, The assay medium was prepared by adding 100, 200 ppm).
  • Method Add 100 ⁇ L of spores suspension (about 1 ⁇ 10 5 cells / mL) of A.
  • Example 1 Target fungus for controlling storage diseases : blue mold (Penicillium italicum) and green mold (Penicillium digitatum) Tested fruits: Wenzhou mandarin orange (Yuran precocious), each group 10 fruits 3 repetitions Method: After scratching about 2 mm in depth with a needle that five insect pins are bundled in 4 parts of each fruit, blue mold fungus and green mold fungus The spore suspension (1 ⁇ 10 5 cfu / mL) was spray inoculated.
  • the fruits were pressed into a predetermined concentration of each drug solution (10 L) in a bucket and dipped for 2 minutes so that all the fruit surfaces including the above-mentioned bellflower parts were fully immersed in the solution. After the same immersion treatment, the fruits were taken out, spread out so as not to overlap, dried overnight (17 hours), packed in a container and stored. Seven days after fungal inoculation, the number of rotten fruits was examined. The fruit which was rotted at one or more of the four locations was regarded as rotten fruit.
  • Example 2 Target fungus for controlling storage diseases: Botrytis cinerea (Botrytis cinerea) Tested fruits: Green peppers, 10 fruits each Method: After scratching with 2 parts per 1 green pepper, a spore suspension (1 ⁇ 10 5 cfu / mL) of the target fungus was spray-inoculated. After 4 hours, it was pressed into an 80 ppm aqueous solution of peracetic acid (10 L) and dipped for 5 seconds so that all the fruit surfaces including the bell parts were fully immersed in the solution. After the same immersion treatment, the fruits were taken out, spread out so as not to overlap, air-dried for 3 hours, packed in a container and stored in a room at 20 ° C.
  • Botrytis cinerea Botrytis cinerea
  • Tested fruits Green peppers, 10 fruits each Method: After scratching with 2 parts per 1 green pepper, a spore suspension (1 ⁇ 10 5 cfu / mL) of the target fungus was spray-ino
  • Example 3 Target bacteria for controlling storage diseases : blue mold (Penicillium italicum) and green mold (Penicillium digitatum)
  • Test fruits Wenzhou mandarin orange (Kansan Wenzhou), each section fruit 10 kg 5 repetition Method: test fruits, each drug solution (80 ppm peracetic acid aqueous solution, 200 ppm sodium hypochlorite aqueous solution or water solution of predetermined concentration in the container)
  • the whole fruit surface including the above-mentioned bellflower part was dipped in water for 2 minutes so that it was fully immersed in the solution.
  • the fruits were removed, spread on newsprint and allowed to dry overnight without overlapping.
  • the fruits were then packed in 10 kg cardboard boxes and stored in a cold air storage set at 8 ° C.
  • the number of rotten fruits after 59 days was investigated and the rot fruit rate was calculated.
  • the decayed fruit rate of the test fruit soaked in 80 ppm peracetic acid aqueous solution is 0%, and the test treated with other agents (200 ppm aqueous sodium hypochlorite solution and tap water) It was confirmed that it was significantly suppressed compared to the fruit rot fruit rate.
  • the results are shown in Tables 7-9 and FIG.
  • the method of the present invention is an inexpensive and safe method applicable to harvested fruits, since it uses peracetic acid as a food additive at very low concentrations as a food additive.
  • the sterilization method of the present invention is easily carried out by immersing the fruit in a germicide, spraying the germicide on the fruit, and the like, and the subsequent washing step of the germicide is unnecessary. It can be easily implemented by operation.
  • the method of the present invention is superior in that the bactericidal activity of filamentous fungi is high and storage diseases can be significantly suppressed during storage and / or transportation periods of several days to several weeks required for domestic remote location or overseas supply. It is

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  • Life Sciences & Earth Sciences (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Agronomy & Crop Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Storage Of Fruits Or Vegetables (AREA)

Abstract

L'invention fournit un procédé d'inhibition de maladie de conservation des fruits ne mettant pas en œuvre de pesticide. Plus précisément, l'invention fournit un procédé qui est caractéristique en ce qu'il inclut une étape au cours de laquelle un fruit récolté pédoncule compris, est mis en contact, avec un agent bactéricide contenant un acide peracétique selon une concentration supérieure ou égale à 10ppm et inférieure à 100ppm, avant sa conservation et ou son transport. Selon l'invention, il est possible d'inhiber de manière significative la maladie de conservation des fruits pendant un intervalle de plusieurs jours à plusieurs semaine nécessaire à un acheminement dans une région éloignée du pays ou à l'étranger et/ou pendant la durée du transport.
PCT/JP2018/039923 2017-11-01 2018-10-26 Procédé d'inhibition de maladie de conservation des fruits mettant en œuvre un acide peracétique WO2019087972A1 (fr)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2019550328A JP7321434B2 (ja) 2017-11-01 2018-10-26 過酢酸を用いた果実の貯蔵病害の抑制方法
CN201880065624.8A CN111200936A (zh) 2017-11-01 2018-10-26 使用过乙酸抑制果实的贮藏病害的方法

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JP2017-211873 2017-11-01

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112088934A (zh) * 2020-08-19 2020-12-18 贵州省果树科学研究所(贵州省柑橘研究所、贵州省特色果蔬工程技术中心、贵州省火龙果研究所) 延长火龙果贮期的防病保鲜方法
CN113080248A (zh) * 2021-05-18 2021-07-09 华中农业大学 柑橘采后快速验伤及同时抑菌的方法
KR20230027667A (ko) 2021-08-19 2023-02-28 전남대학교산학협력단 배 과피얼룩 방제용 조성물 및 상기 조성물을 이용한 배과피 얼룩 방제시스템

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JPH02142709A (ja) * 1988-11-24 1990-05-31 Kurita Water Ind Ltd 有害生物防除剤
JPH10218718A (ja) * 1997-02-05 1998-08-18 Norin Suisansyo Yasai Chiyagiyou Shikenjo 抗菌性組成物並びに青果類の貯蔵病害の制御方法
WO2012027258A2 (fr) * 2010-08-23 2012-03-01 Biosafe Systems Llc Procédé de désinfection d'objets
JP2013515072A (ja) * 2009-12-21 2013-05-02 フレッシュ・エクスプレス・インコーポレイテッド 過酸および２−ヒドロキシ有機酸組成物での物品の殺菌

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CN105394171A (zh) * 2015-12-04 2016-03-16 张家港市金阳生态农业科技有限公司 一种延长柑橘果实贮存期的方法

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Publication number Priority date Publication date Assignee Title
JPH02142709A (ja) * 1988-11-24 1990-05-31 Kurita Water Ind Ltd 有害生物防除剤
JPH10218718A (ja) * 1997-02-05 1998-08-18 Norin Suisansyo Yasai Chiyagiyou Shikenjo 抗菌性組成物並びに青果類の貯蔵病害の制御方法
JP2013515072A (ja) * 2009-12-21 2013-05-02 フレッシュ・エクスプレス・インコーポレイテッド 過酸および２−ヒドロキシ有機酸組成物での物品の殺菌
WO2012027258A2 (fr) * 2010-08-23 2012-03-01 Biosafe Systems Llc Procédé de désinfection d'objets

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KATO, MITSUHIRO ET AL.: "Reduction effect of citrus storage disease by food additives", ABSTRACTS OF PSJ ANNUAL MEETING 2017, vol. 3, no. 29, 12 April 2017 (2017-04-12), pages 79 *
OKOSHI, TOSHIYUKI: "Characteristics and effectiveness of peracetic acid preparation", JAPAN FOOD SCIENCE, 2016, pages 16 - 22 *

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN112088934A (zh) * 2020-08-19 2020-12-18 贵州省果树科学研究所(贵州省柑橘研究所、贵州省特色果蔬工程技术中心、贵州省火龙果研究所) 延长火龙果贮期的防病保鲜方法
CN112088934B (zh) * 2020-08-19 2023-07-18 贵州省果树科学研究所(贵州省柑橘研究所、贵州省特色果蔬工程技术中心、贵州省火龙果研究所) 延长火龙果贮期的防病保鲜方法
CN113080248A (zh) * 2021-05-18 2021-07-09 华中农业大学 柑橘采后快速验伤及同时抑菌的方法
CN113080248B (zh) * 2021-05-18 2022-09-13 华中农业大学 柑橘采后快速验伤及同时抑菌的方法
KR20230027667A (ko) 2021-08-19 2023-02-28 전남대학교산학협력단 배 과피얼룩 방제용 조성물 및 상기 조성물을 이용한 배과피 얼룩 방제시스템
KR20240010077A (ko) 2021-08-19 2024-01-23 전남대학교산학협력단 배과피 얼룩 방제용 조성물 및 상기 조성물을 이용한 배과피 얼룩 제거방법

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CN111200936A (zh) 2020-05-26
JP7321434B2 (ja) 2023-08-07

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