WO2019059702A3 - 나노섬유 기반 장기간 초대 간세포 3차원 배양시스템 및 배양방법 - Google Patents

나노섬유 기반 장기간 초대 간세포 3차원 배양시스템 및 배양방법 Download PDF

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Publication number
WO2019059702A3
WO2019059702A3 PCT/KR2018/011215 KR2018011215W WO2019059702A3 WO 2019059702 A3 WO2019059702 A3 WO 2019059702A3 KR 2018011215 W KR2018011215 W KR 2018011215W WO 2019059702 A3 WO2019059702 A3 WO 2019059702A3
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WO
WIPO (PCT)
Prior art keywords
culture system
drug
primary hepatocyte
primary
liver
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PCT/KR2018/011215
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English (en)
French (fr)
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WO2019059702A2 (ko
WO2019059702A9 (ko
Inventor
곽종영
송민규
김소희
최민호
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아주대학교산학협력단
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Priority claimed from KR1020180110880A external-priority patent/KR102062465B1/ko
Application filed by 아주대학교산학협력단 filed Critical 아주대학교산학협력단
Priority to US16/649,986 priority Critical patent/US11685900B2/en
Publication of WO2019059702A2 publication Critical patent/WO2019059702A2/ko
Publication of WO2019059702A3 publication Critical patent/WO2019059702A3/ko
Publication of WO2019059702A9 publication Critical patent/WO2019059702A9/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

본 발명은 나노섬유 기반 장기간 초대 간세포 배양시스템 및 배양방법에 관한 것으로, 상기 초대 간세포 배양시스템은 나노섬유로 이루어진 지지체를 사이에 두고 인간 초대 간세포와 간 비실질세포를 이격시켜 직접 공배양하지 않고 간접적으로 공배양함으로써 증식성이 낮은 초대 간세포를 장기간 본래의 생리활성을 유지하도록 in vitro 상에서 3차원 세포배양을 할 수 있는 장점이 있다. 이러한 초대 간세포 배양시스템은 간조직 유사 환경으로서 고가의 혈청을 사용하지 않고 저가의 비용으로 손쉽게 초대 간세포를 배양할 수 있으며, 간 약물대사효소(시토크롬 P450 효소) 유도 및 억제를 통한 약물-약물 간 상호작용 분석, 간 수송체의 기능적 업테이크(uptake) 분석 및 약물 유도 간 손상 분석 등 다양한 분야에 활용할 수 있다.
PCT/KR2018/011215 2017-09-25 2018-09-21 나노섬유 기반 장기간 초대 간세포 3차원 배양시스템 및 배양방법 WO2019059702A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/649,986 US11685900B2 (en) 2017-09-25 2018-09-21 Nanofiber-based long-term primary hepatocyte three-dimensional culture system and culturing method

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR20170123545 2017-09-25
KR10-2017-0123545 2017-09-25
KR1020180110880A KR102062465B1 (ko) 2017-09-25 2018-09-17 나노섬유 기반 장기간 초대 간세포 3차원 배양시스템 및 배양방법
KR10-2018-0110880 2018-09-17

Publications (3)

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WO2019059702A2 WO2019059702A2 (ko) 2019-03-28
WO2019059702A3 true WO2019059702A3 (ko) 2019-08-08
WO2019059702A9 WO2019059702A9 (ko) 2019-10-03

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Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102176892B1 (ko) * 2019-03-05 2020-11-10 아주대학교산학협력단 세포특이적 부착능을 증강시키는 폴리비닐 알코올 나노섬유 멤브레인 제조 방법

Citations (4)

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WO2003082145A2 (en) * 2002-03-25 2003-10-09 The General Hospital Corporation Fabrication of vascularized tissue using microfabricated two-dimensional molds
US20060019326A1 (en) * 2003-01-16 2006-01-26 Vacanti Joseph P Use of three-dimensional microfabricated tissue engineered systems for pharmacologic applications
KR101458425B1 (ko) * 2014-04-03 2014-11-07 동아대학교 산학협력단 3차원 구조의 다단 세포배양 구조체, 이를 포함하는 에세이칩 및 에세이칩을 이용한 세포 분석방법
WO2017126758A1 (ko) * 2016-01-21 2017-07-27 한국화학연구원 3차원 간세포 배양 유닛, 간독성 평가 시스템 및 이를 이용한 간독성 평가 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
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WO2003082145A2 (en) * 2002-03-25 2003-10-09 The General Hospital Corporation Fabrication of vascularized tissue using microfabricated two-dimensional molds
US20060019326A1 (en) * 2003-01-16 2006-01-26 Vacanti Joseph P Use of three-dimensional microfabricated tissue engineered systems for pharmacologic applications
KR101458425B1 (ko) * 2014-04-03 2014-11-07 동아대학교 산학협력단 3차원 구조의 다단 세포배양 구조체, 이를 포함하는 에세이칩 및 에세이칩을 이용한 세포 분석방법
WO2017126758A1 (ko) * 2016-01-21 2017-07-27 한국화학연구원 3차원 간세포 배양 유닛, 간독성 평가 시스템 및 이를 이용한 간독성 평가 방법

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GOONOO, N ET AL.: "In vitro and in vivo cytocompatibility of electrospun nanofiber scaffolds for tissue engineering applications", RSC ADV., vol. 4, no. 60, 2014, pages 31618 - 31642, XP055628130 *
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ZHANG, WEIJIE ET AL.: "Electrospinning of fucoidan/chitosan/poly(vinyl alcohol) scaffolds for vascular tissue engineering", FIBERS AND POLYMERS, vol. 18, no. 5, May 2017 (2017-05-01), pages 922 - 932, XP036247073, doi:10.1007/s12221-017-1197-3 *

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WO2019059702A2 (ko) 2019-03-28
WO2019059702A9 (ko) 2019-10-03

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