WO2019054840A2 - Composition for controlling plant viruses, and use thereof - Google Patents

Composition for controlling plant viruses, and use thereof Download PDF

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Publication number
WO2019054840A2
WO2019054840A2 PCT/KR2018/010954 KR2018010954W WO2019054840A2 WO 2019054840 A2 WO2019054840 A2 WO 2019054840A2 KR 2018010954 W KR2018010954 W KR 2018010954W WO 2019054840 A2 WO2019054840 A2 WO 2019054840A2
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virus
composition
plant
butanediol
controlling
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PCT/KR2018/010954
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French (fr)
Korean (ko)
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WO2019054840A3 (en
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신택수
김태훈
박태현
하판정
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주식회사 팜한농
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Publication of WO2019054840A2 publication Critical patent/WO2019054840A2/en
Publication of WO2019054840A3 publication Critical patent/WO2019054840A3/en

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/12—Powders or granules
    • A01N25/14—Powders or granules wettable
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds

Definitions

  • the present invention relates to a composition for controlling plant viruses containing 2,3-butanediol and a method for controlling plant viruses using the same.
  • a virus is a living organism that infects and proliferates a host cell and can not be considered apart from the host.
  • viruses such as animal viruses, plant viruses, bacterial viruses, and fungal viruses depending on the host.
  • plant viruses are collectively referred to as viruses that infect plant cells.
  • plant viruses are composed of non-cells and have non-biological characteristics such as not being able to metabolize themselves without enzymes.
  • plant viruses are replicated using host cells, so they damage various plant populations and share host cells and cellular processes, making it difficult to inhibit the function of plant viruses (Yanmei Li et al., Proc Natl Acad Sci USA 104: 8083-8088, 2007). For this reason, despite the fact that the plant virus was first discovered in the 1890's, physical or indirect control methods such as removing the diseased plant from the package or controlling the pathogens are now widely used, The development of viral agents is minimal.
  • It is an object of the present invention to provide a plant virus control composition comprising 2,3-butanediol as an active ingredient and a pesticide composition for controlling plant viruses containing the same.
  • One aspect of the present invention provides a composition for controlling a plant virus comprising 2,3-butanediol as an active ingredient.
  • the plant virus is selected from the group consisting of cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), pepper mottle virus (PepMoV), tomato spotting virus (Tomato Spotted Wilt Virus, TSWV) and Tomato yellow leaf curl virus (TYLCV).
  • CMV cucumber mosaic virus
  • TMV tobacco mosaic virus
  • PepMoV pepper mottle virus
  • TSWV tomato spotting virus
  • Tomato yellow leaf curl virus Tomato yellow leaf curl virus
  • the present invention is not limited thereto.
  • the concentration of 2,3-butanediol contained in the composition for controlling plant viruses is appropriately determined by a person skilled in the art in consideration of the degree of plant growth, the cultivation environment, Lt; / RTI >
  • the 2,3-butanediol may be included in an amount of 0.001 to 60%, 0.05% to 55%, 0.1% to 50%, 0.5% to 50%, or 1% to 50% But is not limited thereto.
  • the composition for controlling a plant virus may be in the form of, for example, directly sprayable solutions, powders and suspensions, or in the form of highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, Materials or granules, but are not limited thereto.
  • the plant virus control composition may be formulated into a form selected from the group consisting of a liquid agent, a granule, a powder, an oil, an oil, a wetting agent and a coating agent.
  • Another aspect of the present invention provides a pesticide composition for controlling plant viruses, which comprises the composition for controlling plant viruses.
  • Another aspect of the present invention provides a method for controlling a plant virus, comprising the step of treating the plant virus control composition with a plant or soil.
  • composition containing 2,3-butanediol of the present invention has an antiviral activity against various plant viruses, it can be effectively used for controlling plant viruses.
  • Fig. 1 is a photograph showing the degree of occurrence of plant virus according to the test agent treated with pepper.
  • Fig. 2 is a photograph showing the degree of the occurrence of plant virus according to the treatment agent and treatment concentration in red pepper.
  • One aspect of the present invention provides a composition for controlling a plant virus comprising 2,3-butanediol as an active ingredient.
  • the inventors of the present invention have conducted studies on substances having excellent antiviral activity against plant viruses. As a result, 2,3-butanediol was found to be effective against viruses such as cucumber mosaic virus and tobacco mosaic virus And the present invention has been completed.
  • control is interpreted to include prevention of plant virus infection and inhibition of plant virus propagation following disease development.
  • the plant virus is selected from the group consisting of cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), pepper mottle virus (PepMoV), tomato spotting virus (Tomato Spotted Wilt Virus, TSWV) and Tomato yellow leaf curl virus (TYLCV).
  • CMV cucumber mosaic virus
  • TMV tobacco mosaic virus
  • PepMoV pepper mottle virus
  • TSWV tomato spotting virus
  • Tomato yellow leaf curl virus Tomato yellow leaf curl virus
  • the present invention is not limited thereto.
  • the concentration of 2,3-butanediol contained in the composition for controlling plant viruses is appropriately determined by a person skilled in the art in consideration of the degree of plant growth, the cultivation environment, Lt; / RTI >
  • the 2,3-butanediol may be included in an amount of 0.001 to 60%, 0.05% to 55%, 0.1% to 50%, 0.5% to 50%, or 1% to 50% But is not limited thereto.
  • the 2,3-butanediol may be prepared by reacting 2R, 3R-butanediol, 2R, 3S-butanediol, 2S, 3R-butanediol, 2S, 3S- .
  • the 2,3-butanediol may be a single type 2,3-butanediol mixture consisting of only one of the above-mentioned forms, or a mixture of 2,3-butanediol having a specific type of 2,3-butanediol in an amount of 50% , 70% or more, 80% or more, 90% or more, or 95% or more of the total amount of the 2,3-butanediol.
  • the composition for controlling a plant virus may be in the form of, for example, directly sprayable solutions, powders and suspensions, or in the form of highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, Materials or granules, but are not limited thereto.
  • the plant virus control composition may be formulated into a form selected from the group consisting of a liquid agent, a granule, a powder, an oil, an oil, a wetting agent and a coating agent.
  • the plant virus control composition may be used by spraying, spraying, spraying, scattering or swelling. The mode of use of the composition depends on the intended purpose, but in all cases the composition according to the invention must be finely and uniformly distributed.
  • composition for controlling a plant virus of the present invention may be formulated into various forms, for example, by adding a solvent and / or a carrier. Suitable inert additives and surface-active materials, such as emulsifiers or dispersants, may be incorporated into the formulation.
  • Suitable surface-active materials include, but are not limited to, aromatic sulfonic acids (e.g., lignosulfonic acid, phenol-sulfonic acid, naphthalene- and dibutylnaphthalenesulfonic acid), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, A salt of a fatty alcohol glycol ether, a sulfonate naphthalene and derivatives thereof, a condensate of formaldehyde, a condensate of naphthalene or naphthalene sulfonic acid, a phenol and a formaldehyde Condensates, polyoxyethylene octylphenol ethers, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ethers, alkylaryl
  • Suitable solid carrier materials are in principle all porous and comprise an agriculturally acceptable carrier such as mineral earths such as silica, silica gel, silicate, talc, kaolin, limestone, limestone, chalk, Fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, vegetable products such as cereal flour, bark powder, wood meal, And nutshell powder) or cellulose powder, but is not limited thereto.
  • the solid carrier may be used alone or in combination of two or more.
  • composition for controlling plant viral diseases can be used in combination with a dispersing agent, a penetrating agent, or a surfactant to improve crop absorption and effect.
  • composition for controlling a plant virus of the present invention can be used for, but not limited to, a tube, a foliar spray, a seed sterilizer or an agricultural equipment sterilizer.
  • composition for controlling a plant virus of the present invention can be used as an active ingredient by mixing 2,3-butanediol alone or in combination with two or more different antiviral substances.
  • Another aspect of the present invention provides a pesticide composition for controlling plant viruses, which comprises the composition for controlling plant viruses.
  • the pesticidal composition may contain, in addition to the active ingredient, 2,3-butanediol, general components (for example, solvent, carrier, emulsifier, dispersant, adjuvant, etc.) well known in the art, And the like.
  • general components for example, solvent, carrier, emulsifier, dispersant, adjuvant, etc.
  • Another aspect of the present invention provides a method for controlling a plant virus, comprising the step of treating the plant virus control composition with a plant or soil.
  • Such a treatment method may include, but is not limited to, a foliar treatment, a soil treatment, an immersion treatment, a branch cutting or a treatment method applied to an agricultural equipment disinfection.
  • 2,3-butanediol 99.5%; hereinafter referred to as 2,3-BDO
  • the comparative drugs were Victory Gold (horseradish extract, tannic aicd 35% SC (suspension concentrate)) and Parmemonas oleovorans culture (70% SC), respectively.
  • RT-PCR real-time PCR
  • the leaves of the pepper were collected and the leaf powder was prepared with liquid nitrogen. Trizol solution (Invitrogen, USA) was added to the prepared leaf powder and viral RNA was extracted according to the manufacturer's protocol. CDNA was then synthesized from viral RNA extracted using Oligo (dT) primer (Invitrogen) and random hexamer primer (Invitrogen) according to the manufacturer's protocol. The synthesized cDNA was subjected to RT-PCR using a primer specific for CMV in the following Table 1 and compared with the expression of ubiquitin gene as a house keeping gene of a plant, Respectively. Using the water detection rate as a control, the control value was calculated according to the following equation (1).
  • CMV Cucumber Mosaic Virus
  • TMV Tomato mosaic virus
  • SEQ ID NO: 1 Forward 5'-CGTTGCCGCTATCTCTGCTAT-3 ' SEQ ID NO: 2 Reverse 5'-GGATCCTGCATACTGACAAACC-3 ' Tomato mosaic virus (TMV) SEQ ID NO: 3 Forward 5'-ATGTCTTACAGTATCACTAC-3 ' SEQ ID NO: 4 Reverse 5'-TCAAGTTGCAGGACCAGAGG-3 ' Ubiquitin SEQ ID NO: 5 Forward 5'-ACGTGGATCCATGCAAATCTTTGTGAAGAC-3 ' SEQ ID NO: 6 Reverse 5'-AAAGTCGACTAACCACGGAGACGGAGG-3 '
  • Treated group Test agent Treatment concentration (ppm) Control (%)
  • the treated group 2 treated with 2,3-BDO showed almost no symptoms of virus infection, but the treated group treated with Victory Gold 5 or palmworld-treated group 6 showed symptoms of virus infection.
  • Pepper and cucumber were grown in a greenhouse and 2,3-BDO and other test agents were treated according to the times and methods described in Table 3 below.
  • CMV and TMV were inoculated into cucumber and red pepper, respectively.
  • the virus was quantified by RT-PCR on cucumber and pepper according to the method described in Example 1.
  • the CMV specific primers SEQ ID NOs: 1 and 2
  • TMV specific primers SEQ ID NOs: 3 and 4 described in Table 1 were used, respectively.
  • Test agent Drug efficacy test Control (%) Treatment concentration (ppm) When and how to process CMV TMV pepper cucumber pepper cucumber Control (water treatment) - - - - - - - 2,3-BDO 1,000 Treatment of one growing plant 68.8 58.1 73.8 73.9 200 52.0 74.3 68.0 72.6 100 51.1 4.6 51.6 46.4 Tannic aicd (Victory Gold) 350 Growth period 1 time foliar treatment 28.1 1.8 38.5 11.3 Pseudomonas oleovorans cultures ( PalmWorld ) 1,400 Growth period 1 time foliar treatment 11.6 2.2 19.7 35.9
  • Example 3 Determination of plant virus control activity according to the treatment concentration of 2,3-butanediol
  • TMV was inoculated 7 days after the treatment with the drug, and the degree of virus incidence was investigated 2 weeks after the inoculation of the virus to give an onset coefficient of 0 to 9 according to the extent of the virus.
  • the disease severity was determined according to each of the treatment groups according to the following equation (2), and then the control value was determined according to equation (3).
  • Treated group Test agent Treatment concentration (ppm) Control (%) One Control group 1 (TMV non-vaccinated) - 2 Control group 2 (TMV inoculation) - [51.1] 3 2,3-BDO 100 100.0 4 200 89.1 5 400 89.1 6 Pseudomonas oleovorans cultures ( PalmWorld ) 1,400 19.6 7 2,800 73.9 8 Tannic aicd (Victory Gold) 350 26.1 9 700 63.0
  • the red pepper was formulated at a farmhouse in Geumsan-gun, Chungnam province and treated with 2,3-BDO or BTH (benzothiadiazole) three times at regular intervals of 1 month after 7 days.
  • BTH benzothiadiazole
  • TSWV Tomato Spotted Wilt Virus
  • TYLCV Tomato yellow leaf curl virus
  • the leaves of the pepper were collected and the leaf powder was prepared with liquid nitrogen. Trizol solution (Invitrogen, USA) was added to the prepared leaf powder and viral RNA was extracted according to the manufacturer's protocol. CDNA was then synthesized from viral RNA extracted using Oligo (dT) primer (Invitrogen) and random hexamer primer (Invitrogen) according to the manufacturer's protocol. RT-PCR was performed using the synthesized cDNA as a template using the primers specific for CMV, TMV, PepMoV, TSWV or TYLCV described in Table 1 and Table 5 below, and the ubiquitin (ubiquitin) gene expression was analyzed. Using the amount of virus detected, water control was used as a control and the control value was calculated according to the formula (1).
  • Pepper spotted virus SEQ ID NO: 7 Forward 5'-AAGATCAGACACATGGA-3 ' SEQ ID NO: 8 Reverse 5'-CAAGCAAGGGTATGCATGT-3 ' Tomato spots virus (TSWV) SEQ ID NO: 9 Forward 5'-ATGTCTAAGGTTAAGCTCAC-3 ' SEQ ID NO: 10 Reverse 5'-TCAAGCAAGTTCTGCGAGTT-3 ' Tomato yellow leaf blight virus (TYLCV) SEQ ID NO: 11 Forward 5'-CGCCCGCCTCGAAGGTTC-3 ' SEQ ID NO: 12 Reverse 5'-TCGTCGCTTGTTTGTGCCTTG-3 '
  • Test agent Drug efficacy test Control (%) Treatment concentration When and how to process CMV TMV TSWV TYLCV PepMoV 2,3-BDO 100 ppm 3-period treatment of growing season 59.3 98.7 100.0 100.0 90.3 BTH 1 mM 3-period treatment of growing season 67.2 92.4 74.8 99.1 42.8 No treatment - - 45.8 *

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Abstract

The present invention relates to a composition which is for controlling plant viruses and includes 2,3-butanediol. The composition can effectively suppress infection by plant viruses, and thus can be usefully used for controlling plant viruses.

Description

식물바이러스 방제용 조성물 및 이의 용도Composition for controlling plant virus and its use
본 발명은 2,3-부탄다이올을 포함하는 식물바이러스 방제용 조성물 및 이를 이용한 식물바이러스 방제 방법에 관한 것이다.The present invention relates to a composition for controlling plant viruses containing 2,3-butanediol and a method for controlling plant viruses using the same.
바이러스는 숙주세포를 감염시켜 증식하는 생명체로서 숙주와 별도로 그 존재를 생각할 수 없는 생명체이며, 숙주에 따라 동물바이러스, 식물바이러스, 세균바이러스, 곰팡이바이러스 등 여러 종류가 존재한다. 그 중에서 식물바이러스는 식물세포를 감염시키는 바이러스를 총칭한다. 식물바이러스는 완전한 생명체 단위의 균류와 달리 비세포로 구성되어 있고, 효소가 없어 스스로 물질 대사를 못하는 등의 비생물적 특징을 지닌다.A virus is a living organism that infects and proliferates a host cell and can not be considered apart from the host. There are various kinds of viruses such as animal viruses, plant viruses, bacterial viruses, and fungal viruses depending on the host. Among them, plant viruses are collectively referred to as viruses that infect plant cells. Unlike the fungi of whole living organisms, plant viruses are composed of non-cells and have non-biological characteristics such as not being able to metabolize themselves without enzymes.
특히 식물바이러스는 숙주의 세포(host cell)를 이용하여 복제하기 때문에 다양한 식물 개체에 피해를 입히며, 숙주 개체와 세포 과정(cellular process)을 공유하기 때문에 식물바이러스의 기능만을 저해하는데 어려움이 따른다(Yanmei Li et al., Proc. Natl. Acad. Sci. U. S. A. 104:8083-8088, 2007). 상기 이유로 식물바이러스는 1890년대에 그 존재가 처음 밝혀졌음에도 불구하고, 발병된 식물을 포장에서 제거하거나 매개충을 방제하는 등의 물리적 또는 간접적 방제 방법이 현재 널리 쓰이고 있을 뿐, 식물바이러스병에 대한 항바이러스제의 개발은 미비한 수준이다.In particular, plant viruses are replicated using host cells, so they damage various plant populations and share host cells and cellular processes, making it difficult to inhibit the function of plant viruses (Yanmei Li et al., Proc Natl Acad Sci USA 104: 8083-8088, 2007). For this reason, despite the fact that the plant virus was first discovered in the 1890's, physical or indirect control methods such as removing the diseased plant from the package or controlling the pathogens are now widely used, The development of viral agents is minimal.
본 발명의 일 목적은 2,3-부탄다이올(2,3-butanediol)을 유효성분으로 포함하는 식물바이러스 방제용 조성물과 이를 포함하는 식물바이러스 방제용 농약 조성물을 제공하는 것이다.It is an object of the present invention to provide a plant virus control composition comprising 2,3-butanediol as an active ingredient and a pesticide composition for controlling plant viruses containing the same.
본 발명의 다른 목적은 상기 식물바이러스 방제용 조성물을 이용한 식물바이러스 방제 방법을 제공하는 것이다.It is another object of the present invention to provide a plant virus control method using the plant virus control composition.
본 발명의 일 양상은 2,3-부탄다이올을 유효성분으로 포함하는 식물바이러스 방제용 조성물을 제공한다.One aspect of the present invention provides a composition for controlling a plant virus comprising 2,3-butanediol as an active ingredient.
본 발명의 일 실시예에 따르면 상기 식물바이러스는 오이 모자이크 바이러스 (cucumber mosaic virus, CMV), 담배 모자이크 바이러스 (tobacco mosaic virus, TMV), 고추얼룩무늬바이러스 (Pepper mottle virus, PepMoV), 토마토반점시듦바이러스 (Tomato Spotted Wilt Virus, TSWV) 및 토마토황화잎말림바이러스 (Tomato yellow leaf curl virus, TYLCV)로 이루어진 군에서 하나 이상 선택될 수 있으나, 이에 한정하지 않는다.According to one embodiment of the present invention, the plant virus is selected from the group consisting of cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), pepper mottle virus (PepMoV), tomato spotting virus (Tomato Spotted Wilt Virus, TSWV) and Tomato yellow leaf curl virus (TYLCV). However, the present invention is not limited thereto.
본 발명의 일 실시예에 따르면 상기 식물바이러스 방제용 조성물에 포함되는 2,3-부탄다이올의 농도는 식물체의 생육 정도, 경작지 환경, 식물바이러스병의 발병 정도 등을 고려하여 당업자에 의해 적절하게 조절될 수 있다. 바람직하게는, 상기 2,3-부탄다이올은 조성물 전체 중량 대비 0.001 내지 60%, 0.05% 내지 55%, 0.1% 내지 50%, 0.5% 내지 50% 또는 1% 내지 50%로 포함될 수 있지만 이에 한정되는 것은 아니다.According to one embodiment of the present invention, the concentration of 2,3-butanediol contained in the composition for controlling plant viruses is appropriately determined by a person skilled in the art in consideration of the degree of plant growth, the cultivation environment, Lt; / RTI > Preferably, the 2,3-butanediol may be included in an amount of 0.001 to 60%, 0.05% to 55%, 0.1% to 50%, 0.5% to 50%, or 1% to 50% But is not limited thereto.
본 발명의 일 실시예에 따르면 상기 식물바이러스 방제용 조성물은 예를 들어 직접 분사 가능한 용액, 분말 및 현탁액의 형태 또는 고농축 수성, 유성 또는 다른 현탁액, 분산액, 에멀젼, 유성 분산액, 페이스트, 분진, 흩뿌림 물질 또는 과립제로 제조할 수 있으나, 이에 한정하지 않는다. 바람직하게는 상기 식물바이러스 방제용 조성물은 액제, 입제, 분제, 유제, 오일제, 수화제 및 도포제로 이루어진 군에서 선택되는 형태로 제형화될 수 있다. According to one embodiment of the present invention, the composition for controlling a plant virus may be in the form of, for example, directly sprayable solutions, powders and suspensions, or in the form of highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, Materials or granules, but are not limited thereto. Preferably, the plant virus control composition may be formulated into a form selected from the group consisting of a liquid agent, a granule, a powder, an oil, an oil, a wetting agent and a coating agent.
본 발명의 다른 양상은 상기 식물바이러스 방제용 조성물을 포함하는 식물바이러스 방제용 농약 조성물을 제공한다.Another aspect of the present invention provides a pesticide composition for controlling plant viruses, which comprises the composition for controlling plant viruses.
본 발명의 다른 양상은 상기 식물바이러스 방제용 조성물을 식물체 또는 토양에 처리하는 단계를 포함하는 식물바이러스의 방제 방법을 제공한다.Another aspect of the present invention provides a method for controlling a plant virus, comprising the step of treating the plant virus control composition with a plant or soil.
본 발명의 2,3-부탄다이올을 포함하는 조성물은 다양한 식물바이러스에 대하여 항바이러스 활성을 가지고 있으므로 식물바이러스 방제 용도로 효과적으로 이용될 수 있다.Since the composition containing 2,3-butanediol of the present invention has an antiviral activity against various plant viruses, it can be effectively used for controlling plant viruses.
도 1은 고추에 처리한 시험 약제에 따른 식물바이러스의 발병 정도를 보여주는 사진이다.Fig. 1 is a photograph showing the degree of occurrence of plant virus according to the test agent treated with pepper.
도 2는 고추에서 처리 약제 및 처리 농도에 따른 식물바이러스의 발병 정도를 보여주는 사진이다.Fig. 2 is a photograph showing the degree of the occurrence of plant virus according to the treatment agent and treatment concentration in red pepper.
본 발명의 일 양상은 2,3-부탄다이올을 유효성분으로 포함하는 식물바이러스 방제용 조성물을 제공한다.One aspect of the present invention provides a composition for controlling a plant virus comprising 2,3-butanediol as an active ingredient.
본 발명자들은 식물바이러스에 대한 항바이러스 활성이 우수한 물질에 대하여 연구한 결과, 2,3-부탄다이올이 오이 모자이크 바이러스(cucumber mosaic virus) 및 담배 모자이크 바이러스(tobacco mosaic virus) 등에 대하여 바이러스의 병원성을 소멸시키는 사실을 새롭게 확인하여 본 발명을 완성하였다.The inventors of the present invention have conducted studies on substances having excellent antiviral activity against plant viruses. As a result, 2,3-butanediol was found to be effective against viruses such as cucumber mosaic virus and tobacco mosaic virus And the present invention has been completed.
본 명세서에서 사용되는 용어, "방제(control)"는 식물바이러스에 의한 병해 발생 이전의 예방 및 병해 발생 이후 식물바이러스 증식의 억제를 포함하는 것으로 해석한다.As used herein, the term " control " is interpreted to include prevention of plant virus infection and inhibition of plant virus propagation following disease development.
본 발명의 일 실시예에 따르면 상기 식물바이러스는 오이 모자이크 바이러스 (cucumber mosaic virus, CMV), 담배 모자이크 바이러스 (tobacco mosaic virus, TMV), 고추얼룩무늬바이러스 (Pepper mottle virus, PepMoV), 토마토반점시듦바이러스 (Tomato Spotted Wilt Virus, TSWV) 및 토마토황화잎말림바이러스 (Tomato yellow leaf curl virus, TYLCV)로 이루어진 군에서 하나 이상 선택될 수 있으나, 이에 한정하지 않는다.According to one embodiment of the present invention, the plant virus is selected from the group consisting of cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), pepper mottle virus (PepMoV), tomato spotting virus (Tomato Spotted Wilt Virus, TSWV) and Tomato yellow leaf curl virus (TYLCV). However, the present invention is not limited thereto.
본 발명의 일 실시예에 따르면 상기 식물바이러스 방제용 조성물에 포함되는 2,3-부탄다이올의 농도는 식물체의 생육 정도, 경작지 환경, 식물바이러스병의 발병 정도 등을 고려하여 당업자에 의해 적절하게 조절될 수 있다. 바람직하게는, 상기 2,3-부탄다이올은 조성물 전체 중량 대비 0.001 내지 60%, 0.05% 내지 55%, 0.1% 내지 50%, 0.5% 내지 50% 또는 1% 내지 50%로 포함될 수 있지만 이에 한정되는 것은 아니다.According to one embodiment of the present invention, the concentration of 2,3-butanediol contained in the composition for controlling plant viruses is appropriately determined by a person skilled in the art in consideration of the degree of plant growth, the cultivation environment, Lt; / RTI > Preferably, the 2,3-butanediol may be included in an amount of 0.001 to 60%, 0.05% to 55%, 0.1% to 50%, 0.5% to 50%, or 1% to 50% But is not limited thereto.
본 발명의 일 실시예에 따르면 상기 2,3-부탄다이올은 2R,3R-부탄다이올, 2R,3S-부탄다이올, 2S,3R-부탄다이올 및 2S,3S-부탄다이올 등을 포함할 수 있다. 또한, 상기 2,3-부탄다이올은 상기 가능한 형태들 중 하나로만 이루어진 단일 형태의 2,3-부탄다이올 혼합물이거나, 특정 형태의 2,3-부탄다이올을 50% 이상, 60% 이상, 70% 이상, 80% 이상, 90% 이상 또는 95% 이상 포함하는 복합 형태의 2,3-부탄다이올 혼합물일 수 있다.According to an embodiment of the present invention, the 2,3-butanediol may be prepared by reacting 2R, 3R-butanediol, 2R, 3S-butanediol, 2S, 3R-butanediol, 2S, 3S- . Also, the 2,3-butanediol may be a single type 2,3-butanediol mixture consisting of only one of the above-mentioned forms, or a mixture of 2,3-butanediol having a specific type of 2,3-butanediol in an amount of 50% , 70% or more, 80% or more, 90% or more, or 95% or more of the total amount of the 2,3-butanediol.
본 발명의 일 실시예에 따르면 상기 식물바이러스 방제용 조성물은 예를 들어 직접 분사 가능한 용액, 분말 및 현탁액의 형태 또는 고농축 수성, 유성 또는 다른 현탁액, 분산액, 에멀젼, 유성 분산액, 페이스트, 분진, 흩뿌림 물질 또는 과립제로 제조할 수 있으나, 이에 한정하지 않는다. 바람직하게는 상기 식물바이러스 방제용 조성물은 액제, 입제, 분제, 유제, 오일제, 수화제 및 도포제로 이루어진 군에서 선택되는 형태로 제형화될 수 있다. 또한, 상기 식물바이러스 방제용 조성물은 분사, 분무, 살포, 흩뿌림 또는 붓기에 의해 사용될 수 있다. 상기 조성물의 사용 형태는 의도한 목적에 의존하는데, 모든 경우에 본 발명에 따른 조성물이 미세하고 균일하게 분포되도록 해야 한다.According to one embodiment of the present invention, the composition for controlling a plant virus may be in the form of, for example, directly sprayable solutions, powders and suspensions, or in the form of highly concentrated aqueous, oily or other suspensions, dispersions, emulsions, oily dispersions, pastes, dusts, Materials or granules, but are not limited thereto. Preferably, the plant virus control composition may be formulated into a form selected from the group consisting of a liquid agent, a granule, a powder, an oil, an oil, a wetting agent and a coating agent. In addition, the plant virus control composition may be used by spraying, spraying, spraying, scattering or swelling. The mode of use of the composition depends on the intended purpose, but in all cases the composition according to the invention must be finely and uniformly distributed.
본 발명의 식물바이러스 방제용 조성물은 다양한 형태로 제제화될 수 있으며, 예를 들어 용매 및/또는 담체를 첨가함으로서 제조될 수 있다. 적절한 비활성 첨가제 및 표면-활성 물질, 예를 들어 유화제 또는 분산제를 제제에 혼합할 수 있다. 적합한 표면-활성 물질은 방향족 술폰산(예를 들어 리그노술폰산, 페놀-술폰산, 나프탈렌- 및 디부틸나프탈렌술폰산), 지방산, 알킬- 및 알킬아릴술포네이트, 알킬 라우릴 에테르, 지방 알코올 술페이트의 알칼리 금속, 알카라인 토금속, 암모늄염, 술페이트화 헥사-, 헵타- 및 옥타데칸올, 지방 알코올 글리콜 에테르의 염, 술포네이트 나프탈렌 및 이의 유도체, 포름알데히드의 축합물, 나프탈렌 또는 나프탈렌술폰산, 페놀 및 포름알데히드의 축합물, 폴리옥시에틸렌옥틸 페놀 에테르, 에톡실화 이소옥틸-, 옥틸- 또는 노닐페놀, 알킬페닐 또는 트리부틸페닐 폴리글리콜 에테르, 알킬아릴폴리에테르 알코올, 이소트리데실 알코올, 지방 알코올/에틸렌 옥사이드 축합물, 에톡실화 피마자유, 폴리옥시에틸렌 알킬에테르 또는 폴리옥시프로필렌, 라우릴 알코올 폴리글리콜 에테르 아세테이트, 소르비톨 에스테르, 리그닌-술파이트 폐액 또는 메틸셀룰로오스일 수 있으나, 이에 한정하지 않는다. 적합한 고형 담체 물질은 원칙적으로, 모두 다공성이고, 농업적으로 허용가능한 담체, 예를 들어 광물토류(예컨대 실리카, 실리카 겔, 실리케이트, 활석, 고령토, 석회암, 석회, 초크, 보울, 황토, 점토류, 백운석, 규조 토류, 황산칼슘, 황산 마그네슘, 산화마그네슘, 분쇄 합성물질), 비료(예컨대 황산암모늄, 인산암모늄, 질산암모늄, 우레아), 식물성 제품(예컨대 곡물 가루, 나무 껍질 가루, 목분(wood meal) 및 견과 껍질 가루) 또는 셀룰로오스 분말일 수 있으나, 이에 한정하지 않는다. 또한, 상기 고형 담체는 1종류 또는 2종류 이상을 혼합하여 사용할 수 있다.The composition for controlling a plant virus of the present invention may be formulated into various forms, for example, by adding a solvent and / or a carrier. Suitable inert additives and surface-active materials, such as emulsifiers or dispersants, may be incorporated into the formulation. Suitable surface-active materials include, but are not limited to, aromatic sulfonic acids (e.g., lignosulfonic acid, phenol-sulfonic acid, naphthalene- and dibutylnaphthalenesulfonic acid), fatty acids, alkyl- and alkylarylsulfonates, alkyl lauryl ethers, A salt of a fatty alcohol glycol ether, a sulfonate naphthalene and derivatives thereof, a condensate of formaldehyde, a condensate of naphthalene or naphthalene sulfonic acid, a phenol and a formaldehyde Condensates, polyoxyethylene octylphenol ethers, ethoxylated isooctyl-, octyl- or nonylphenol, alkylphenyl or tributylphenyl polyglycol ethers, alkylaryl polyether alcohols, isotridecyl alcohol, fatty alcohol / ethylene oxide condensates , Ethoxylated castor oil, polyoxyethylene alkyl ether or polyoxypropylene, lauryl alcohol Polyglycol ether acetate, sorbitol esters, lignin-sulfite waste liquid, or may be a cellulose, but not always limited thereto. Suitable solid carrier materials are in principle all porous and comprise an agriculturally acceptable carrier such as mineral earths such as silica, silica gel, silicate, talc, kaolin, limestone, limestone, chalk, Fertilizers such as ammonium sulfate, ammonium phosphate, ammonium nitrate, urea, vegetable products such as cereal flour, bark powder, wood meal, And nutshell powder) or cellulose powder, but is not limited thereto. The solid carrier may be used alone or in combination of two or more.
또한, 상기 식물바이러스 방제용 조성물은 작물체 흡수 및 효과를 증진시키기 위하여 확산제 및 침투제, 또는 계면활성제와도 혼용이 가능하다.In addition, the composition for controlling plant viral diseases can be used in combination with a dispersing agent, a penetrating agent, or a surfactant to improve crop absorption and effect.
본 발명의 식물바이러스 방제용 조성물은 관수용, 엽면 살포용, 종자 소독용 또는 농기구 소독용으로 사용될 수 있으나, 이에 한정하지 않는다.The composition for controlling a plant virus of the present invention can be used for, but not limited to, a tube, a foliar spray, a seed sterilizer or an agricultural equipment sterilizer.
본 발명의 식물바이러스 방제용 조성물은 유효성분으로서 2,3-부탄다이올을 단독, 또는 2종 이상의 다른 항바이러스성 물질 등과 혼합하여 사용할 수 있다.The composition for controlling a plant virus of the present invention can be used as an active ingredient by mixing 2,3-butanediol alone or in combination with two or more different antiviral substances.
본 발명의 다른 양상은 상기 식물바이러스 방제용 조성물을 포함하는 식물바이러스 방제용 농약 조성물을 제공한다.Another aspect of the present invention provides a pesticide composition for controlling plant viruses, which comprises the composition for controlling plant viruses.
상기 농약 조성물은 유효성분인 2,3-부탄다이올 이외에 당업계에 공지된 일반적인 성분(예를 들어, 용매, 담체, 유화제, 분산제, 보조제 등)이 포함될 수 있으며, 식물 전염병에 대한 방제 효과가 있는 농약을 추가로 포함할 수 있다.The pesticidal composition may contain, in addition to the active ingredient, 2,3-butanediol, general components (for example, solvent, carrier, emulsifier, dispersant, adjuvant, etc.) well known in the art, And the like.
본 발명의 다른 양상은 상기 식물바이러스 방제용 조성물을 식물체 또는 토양에 처리하는 단계를 포함하는 식물바이러스의 방제 방법을 제공한다.Another aspect of the present invention provides a method for controlling a plant virus, comprising the step of treating the plant virus control composition with a plant or soil.
상기 처리 방법에는 경엽처리, 토양처리, 침지처리, 가지절단 또는 농기구 소독에 적용하는 처리 방법 등이 포함될 수 있으나, 이에 한정하지 않는다.Such a treatment method may include, but is not limited to, a foliar treatment, a soil treatment, an immersion treatment, a branch cutting or a treatment method applied to an agricultural equipment disinfection.
이하 하나 이상의 구체예를 실시예를 통하여 보다 상세하게 설명한다. 그러나, 이들 실시예는 하나 이상의 구체예를 예시적으로 설명하기 위한 것으로 본 발명의 범위가 이들 실시예에 한정되는 것은 아니다.Hereinafter, one or more embodiments will be described in more detail by way of examples. However, these embodiments are intended to illustrate one or more embodiments, and the scope of the present invention is not limited to these embodiments.
실시예 1: 2,3-부탄다이올(butanediol)의 식물바이러스 방제 활성 확인Example 1: Confirmation of 2,3-butanediol plant virus control activity
온실에서 고추를 재배하여 본엽이 4 내지 5엽 정도로 생장하였을 때 2,3-부탄다이올(99.5%; 이하, 2,3-BDO로 기재함)을 육묘상처리하였다. 비교 약제로는 빅토리골드(오배자 추출물, tannic aicd 35% SC (suspension concentrate; 액상 수화제)) 및 팜월드(Pseudomonas oleovorans 배양물, 70% SC)를 각각 관주처리하였다.When pepper was grown in the greenhouse and the main stem was grown to about 4 to 5 leaves, 2,3-butanediol (99.5%; hereinafter referred to as 2,3-BDO) was treated as a seedling. The comparative drugs were Victory Gold (horseradish extract, tannic aicd 35% SC (suspension concentrate)) and Parmemonas oleovorans culture (70% SC), respectively.
약제 처리 1주일 후 오이 모자이크 바이러스(cucumber mosaic virus; 이하 CMV로 기재함) 및 담배 모자이크 바이러스(tobacco mosaic virus; 이하 TMV로 기재함) 2종의 혼합액을 고추에 접종하였다. 구체적으로 5x5 ㎝ 크기의 이병된 담배잎을 바이러스 감염 버퍼(virus infection buffer) 5 ㎖에 침지한 후 분쇄하여 바이러스 즙액을 제조하고, 고추에 상처를 낸 후 상기 즙액을 접종하였다. 바이러스 접종 2 주일 후, 실시간 PCR (Real-Time PCR, 이하 RT-PCR로 기재함)로 바이러스를 정량하였다.One week after the drug treatment, pepper was inoculated with a mixture of two kinds of cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV). Specifically, a 5x5 cm sized tobacco leaf was immersed in 5 ml of a virus infection buffer and pulverized to prepare a virus juice. The pepper was wounded and the juice was inoculated. Two weeks after the virus inoculation, the virus was quantified by real-time PCR (hereinafter referred to as RT-PCR).
먼저 고추 잎을 채취하여 액체질소로 잎 분말을 제조하였다. 제조된 잎 분말에 Trizol 용액(Invitrogen, 미국)을 첨가하여 제조사의 프로토콜에 따라 바이러스 RNA를 추출하였다. 이후 제조사의 프로토콜에 따라 Oligo(dT) 프라이머(primer)(Invitrogen) 및 random hexamer 프라이머(Invitrogen)를 이용하여 추출한 바이러스 RNA로부터 cDNA를 합성하였다. 합성된 cDNA를 주형으로 하기 표 1 중 CMV에 특이적인 프라이머를 이용하여 RT-PCR을 수행하고, 식물체의 하우스 키핑 유전자(house keeping gene)인 유비퀴틴(ubiquitin) 유전자의 발현 대비 각 약제 처리구의 바이러스 검출량을 분석하였다. 분석한 바이러스 검출량을 이용하여 물 처리구를 대조군으로 하여 하기 수학식 1에 따라 방제가를 산출하였다.First, the leaves of the pepper were collected and the leaf powder was prepared with liquid nitrogen. Trizol solution (Invitrogen, USA) was added to the prepared leaf powder and viral RNA was extracted according to the manufacturer's protocol. CDNA was then synthesized from viral RNA extracted using Oligo (dT) primer (Invitrogen) and random hexamer primer (Invitrogen) according to the manufacturer's protocol. The synthesized cDNA was subjected to RT-PCR using a primer specific for CMV in the following Table 1 and compared with the expression of ubiquitin gene as a house keeping gene of a plant, Respectively. Using the water detection rate as a control, the control value was calculated according to the following equation (1).
바이러스virus 서열order
오이 모자이크 바이러스(CMV)Cucumber Mosaic Virus (CMV) 서열번호 1SEQ ID NO: 1 ForwardForward 5’-CGTTGCCGCTATCTCTGCTAT-3’5'-CGTTGCCGCTATCTCTGCTAT-3 '
서열번호 2SEQ ID NO: 2 ReverseReverse 5’-GGATCCTGCATACTGACAAACC-3’5'-GGATCCTGCATACTGACAAACC-3 '
토마토 모자이크 바이러스(TMV)Tomato mosaic virus (TMV) 서열번호 3SEQ ID NO: 3 ForwardForward 5’-ATGTCTTACAGTATCACTAC-3’5'-ATGTCTTACAGTATCACTAC-3 '
서열번호 4SEQ ID NO: 4 ReverseReverse 5’-TCAAGTTGCAGGACCAGAGG-3’5'-TCAAGTTGCAGGACCAGAGG-3 '
유비퀴틴(Ubiquitin)Ubiquitin 서열번호 5SEQ ID NO: 5 ForwardForward 5’-ACGTGGATCCATGCAAATCTTTGTGAAGAC-3’5'-ACGTGGATCCATGCAAATCTTTGTGAAGAC-3 '
서열번호 6SEQ ID NO: 6 ReverseReverse 5’-AAAGTCGACTAACCACGGAGACGGAGG-3’5'-AAAGTCGACTAACCACGGAGACGGAGG-3 '
Figure PCTKR2018010954-appb-M000001
Figure PCTKR2018010954-appb-M000001
확인 결과, 2,3-BDO의 농도가 증가할수록 오이 모자이크 바이러스 방제 활성이 증가하는 것을 알 수 있었으며, 비교 약제로 사용한 빅토리골드 및 팜월드와 유사하거나 또는 더 우수한 바이러스 방제 활성을 보이는 것을 확인할 수 있었다. 하기 표 2에 각 약제들의 바이러스 방제가를 기재하였으며, 방제가는 상기 수학식 1에 따라 계산하였다.As a result, it was found that the activity of controlling cucumber mosaic virus was increased as the concentration of 2,3-BDO was increased, and it was confirmed that virus control activity similar to or better than Victory Gold and PalmWorld used as comparative drugs . The virus control agents of each agent are shown in Table 2 below, and the control agent was calculated according to the above formula (1).
처리군Treated group 시험 약제Test agent 처리 농도 (ppm)Treatment concentration (ppm) 방제가(%)Control (%)
1One 대조군Control group -- [100.0][100.0]
22 2,3-BDO2,3-BDO 200200 87.087.0
33 100100 41.141.1
44 5050 50.250.2
55 Tannic aicd(빅토리골드)Tannic aicd (Victory Gold) 350350 48.448.4
66 Pseudomonas oleovorans 배양물(팜월드) Pseudomonas oleovorans cultures ( PalmWorld ) 1,4001,400 65.265.2
또한, 바이러스의 발병 정도를 약제 처리 21일 후에 육안으로 확인한 결과, 도 1에서 보는 바와 같이 2,3-BDO를 처리한 처리군 2는 바이러스 발병 증상이 거의 나타나지 않았으나, 빅토리골드를 처리한 처리군 5 또는 팜월드를 처리한 처리군 6은 바이러스 발병 증상이 나타나는 것을 확인할 수 있었다.As shown in FIG. 1, the treated group 2 treated with 2,3-BDO showed almost no symptoms of virus infection, but the treated group treated with Victory Gold 5 or palmworld-treated group 6 showed symptoms of virus infection.
실시예 2: 2,3-부탄다이올의 식물바이러스 방제 활성 확인Example 2: Identification of plant virus control activity of 2,3-butanediol
온실에서 고추 및 오이를 재배하고, 2,3-BDO 및 다른 시험 약제들을 하기 표 3에 기재된 시기 및 방법에 따라 처리하였다. 약제 처리 1주일 후 CMV 및 TMV를 각각 오이 및 고추에 접종하고, 바이러스 접종 2주일 후 실시예 1에 기재된 방법에 따라 오이 및 고추에 대해 RT-PCR로 바이러스를 정량하였다. 각각의 바이러스 검출을 위해 표 1에 기재된 CMV 특이적 프라이머(서열번호 1 및 2)와 TMV 특이적 프라이머(서열번호 3 및 4)를 각각 사용하였다.Pepper and cucumber were grown in a greenhouse and 2,3-BDO and other test agents were treated according to the times and methods described in Table 3 below. One week after the drug treatment, CMV and TMV were inoculated into cucumber and red pepper, respectively. Two weeks after the inoculation of the virus, the virus was quantified by RT-PCR on cucumber and pepper according to the method described in Example 1. For each virus detection, the CMV specific primers (SEQ ID NOs: 1 and 2) and TMV specific primers (SEQ ID NOs: 3 and 4) described in Table 1 were used, respectively.
분석한 바이러스 검출량을 이용하여 물 처리구를 대조군으로 하여 상기 수학식 1에 따라 방제가를 산출하였다. 그 결과, 하기 표 3에 나타난 것과 같이 2,3-BDO가 농도 의존적으로 고추 및 오이에서 CMV 및 TMV 모두에 대하여 방제 활성을 가지는 것을 알 수 있었다.Using the amount of virus detected, water control was used as a control and the control value was calculated according to the formula (1). As a result, as shown in Table 3 below, it was found that 2,3-BDO had a controlling activity against both CMV and TMV in pepper and cucumber in a concentration-dependent manner.
시험 약제Test agent 약효시험Drug efficacy test 방제가(%)Control (%)
처리농도 (ppm)Treatment concentration (ppm) 처리시기및 방법When and how to process CMVCMV TMVTMV
고추pepper 오이cucumber 고추pepper 오이cucumber
대조군(물 처리)Control (water treatment) -- -- -- -- -- --
2,3-BDO2,3-BDO 1,0001,000 생육기 1회 관주처리Treatment of one growing plant 68.868.8 58.158.1 73.873.8 73.973.9
200200 52.052.0 74.374.3 68.068.0 72.672.6
100100 51.151.1 4.64.6 51.651.6 46.446.4
Tannic aicd (빅토리골드)Tannic aicd (Victory Gold) 350350 생육기 1회 경엽처리Growth period 1 time foliar treatment 28.128.1 1.81.8 38.538.5 11.311.3
Pseudomonas oleovorans 배양물(팜월드) Pseudomonas oleovorans cultures ( PalmWorld ) 1,4001,400 생육기 1회 경엽처리Growth period 1 time foliar treatment 11.611.6 2.22.2 19.719.7 35.935.9
실시예 3: 2,3-부탄다이올의 처리 농도에 따른 식물바이러스 방제 활성 확인Example 3: Determination of plant virus control activity according to the treatment concentration of 2,3-butanediol
온실에 고추를 파종하여 하기 표 4에 기재된 약제들을 농도별로 유묘기 고추에 각각 처리하였다. 약제 처리 7일 후 TMV를 접종하고, 바이러스 접종 2주 후에 바이러스 발병 정도를 달관조사하여 바이러스의 발병 정도에 따라 0 내지 9의 발병계수를 부여하였다. 하기 수학식 2에 따라 각 처리군 별 발병도를 결정한 후, 수학식 3 에 따라 방제가를 결정하였다.The peppers were sown in the greenhouse, and the medicines listed in Table 4 below were each treated in the red pepper at different concentrations. TMV was inoculated 7 days after the treatment with the drug, and the degree of virus incidence was investigated 2 weeks after the inoculation of the virus to give an onset coefficient of 0 to 9 according to the extent of the virus. The disease severity was determined according to each of the treatment groups according to the following equation (2), and then the control value was determined according to equation (3).
Figure PCTKR2018010954-appb-M000002
Figure PCTKR2018010954-appb-M000002
Figure PCTKR2018010954-appb-M000003
Figure PCTKR2018010954-appb-M000003
조사 결과, 하기 표 4에 나타난 것과 같이, 2,3-BDO의 경우 90 내지 100% 수준의 방제 활성을 보이는 것을 확인할 수 있었다. 또한 도 2에서 보는 바와 같이, 팜월드 및 빅토리골드에 비하여 적은 양으로도 우수한 방제 효과를 나타내는 것을 확인할 수 있었다.As a result of the investigation, it was confirmed that 2,3-BDO showed 90 ~ 100% control activity as shown in Table 4 below. Also, as shown in FIG. 2, it was confirmed that the control effect was excellent even in a small amount as compared with PalmWorld and Victory Gold.
처리군Treated group 시험 약제Test agent 처리농도 (ppm)Treatment concentration (ppm) 방제가(%)Control (%)
1One 대조군 1 (TMV 미접종)Control group 1 (TMV non-vaccinated) --
22 대조군 2 (TMV 접종)Control group 2 (TMV inoculation) -- [51.1][51.1]
33 2,3-BDO2,3-BDO 100100 100.0100.0
44 200200 89.189.1
55 400400 89.189.1
66 Pseudomonas oleovorans 배양물(팜월드) Pseudomonas oleovorans cultures ( PalmWorld ) 1,4001,400 19.619.6
77 2,8002,800 73.973.9
88 Tannic aicd(빅토리골드)Tannic aicd (Victory Gold) 350350 26.126.1
99 700700 63.063.0
실시예 4: 5종의 식물바이러스에 대한 2,3-부탄다이올의 방제 활성 확인Example 4: Identification of 2,3-butanediol controlling activity against five plant viruses
충남 금산군 소재 농가에서 고추를 정식하고, 정식 1개월 후 7일 간격으로 2,3-BDO 또는 BTH(benzothiadiazole)를 3회 관주처리하였다. 고추 생육 과정에서 자연발병한 CMV, TMV, PepMoV(Pepper mottle virus, 고추얼룩무늬바이러스), TSWV(Tomato Spotted Wilt Virus, 토마토반점시듦바이러스) 및 TYLCV(Tomato yellow leaf curl virus, 토마토황화잎말림바이러스)에 대하여, 1차 약제 처리 90일 후 RT-PCR로 바이러스를 정량하였다. The red pepper was formulated at a farmhouse in Geumsan-gun, Chungnam Province and treated with 2,3-BDO or BTH (benzothiadiazole) three times at regular intervals of 1 month after 7 days. (Peppermint virus), TSWV (Tomato Spotted Wilt Virus), and Tomato yellow leaf curl virus (TYLCV), which are naturally occurring in the growth of pepper. , The virus was quantified by RT-PCR 90 days after the first drug treatment.
먼저 고추 잎을 채취하여 액체질소로 잎 분말을 제조하였다. 제조된 잎 분말에 Trizol 용액(Invitrogen, 미국)을 첨가하여 제조사의 프로토콜에 따라 바이러스 RNA를 추출하였다. 이후 제조사의 프로토콜에 따라 Oligo(dT) 프라이머(primer)(Invitrogen) 및 random hexamer 프라이머(Invitrogen)를 이용하여 추출한 바이러스 RNA로부터 cDNA를 합성하였다. 합성된 cDNA를 주형으로 상기 표 1 및 하기 표 5에 기재된 CMV, TMV, PepMoV, TSWV 또는 TYLCV에 특이적인 프라이머를 이용하여 RT-PCR을 수행하고, 식물체의 하우스 키핑 유전자(house keeping gene)인 유비퀴틴(ubiquitin) 유전자의 발현 대비 각 약제 처리구의 바이러스 검출량을 분석하였다. 분석한 바이러스 검출량을 이용하여 물 처리구를 대조군으로 하여 상기 수학식 1에 따라 방제가를 산출하였다.First, the leaves of the pepper were collected and the leaf powder was prepared with liquid nitrogen. Trizol solution (Invitrogen, USA) was added to the prepared leaf powder and viral RNA was extracted according to the manufacturer's protocol. CDNA was then synthesized from viral RNA extracted using Oligo (dT) primer (Invitrogen) and random hexamer primer (Invitrogen) according to the manufacturer's protocol. RT-PCR was performed using the synthesized cDNA as a template using the primers specific for CMV, TMV, PepMoV, TSWV or TYLCV described in Table 1 and Table 5 below, and the ubiquitin (ubiquitin) gene expression was analyzed. Using the amount of virus detected, water control was used as a control and the control value was calculated according to the formula (1).
이름name 서열order
고추얼룩무늬바이러스(PepMoV)Pepper spotted virus (PepMoV) 서열번호 7SEQ ID NO: 7 ForwardForward 5’-AAGATCAGACACATGGA-3’5'-AAGATCAGACACATGGA-3 '
서열번호 8SEQ ID NO: 8 ReverseReverse 5’-CAAGCAAGGGTATGCATGT-3’5'-CAAGCAAGGGTATGCATGT-3 '
토마토반점시듦바이러스(TSWV)Tomato spots virus (TSWV) 서열번호 9SEQ ID NO: 9 ForwardForward 5’-ATGTCTAAGGTTAAGCTCAC-3’5'-ATGTCTAAGGTTAAGCTCAC-3 '
서열번호 10SEQ ID NO: 10 ReverseReverse 5’-TCAAGCAAGTTCTGCGAGTT-3’5'-TCAAGCAAGTTCTGCGAGTT-3 '
토마토황화잎말림바이러스(TYLCV)Tomato yellow leaf blight virus (TYLCV) 서열번호 11SEQ ID NO: 11 ForwardForward 5’-CGCCCGCCTCGAAGGTTC-3’5'-CGCCCGCCTCGAAGGTTC-3 '
서열번호 12SEQ ID NO: 12 ReverseReverse 5’-TCGTCGCTTGTTTGTGCCTTG-3’5'-TCGTCGCTTGTTTGTGCCTTG-3 '
방제가를 산출한 결과, 하기 표 6에 나타낸 바와 같이, 2,3-BDO가 상기 5종의 바이러스 모두에 대하여 방제 활성을 가지는 것을 확인할 수 있었다.As shown in Table 6 below, it was confirmed that 2,3-BDO had a controlling activity against all five viruses.
시험약제Test agent 약효시험Drug efficacy test 방제가(%)Control (%)
처리농도Treatment concentration 처리시기및 방법When and how to process CMVCMV TMVTMV TSWVTSWV TYLCVTYLCV PepMoVPepMoV
2,3-BDO2,3-BDO 100 ppm100 ppm 생육기 3회 관주처리3-period treatment of growing season 59.359.3 98.798.7 100.0100.0 100.0100.0 90.390.3
BTHBTH 1 mM1 mM 생육기 3회 관주처리3-period treatment of growing season 67.267.2 92.492.4 74.874.8 99.199.1 42.842.8
무처리No treatment -- -- 45.8* 45.8 *
* 무처리구의 바이러스 발병주율 * Virus incidence rate of non-treatment
이제까지 본 발명에 대하여 그 바람직한 실시예들을 중심으로 살펴보았다. 본 발명이 속하는 기술 분야에서 통상의 지식을 가진 자는 본 발명이 발명의 본질적인 특성에서 벗어나지 않는 범위에서 변형된 형태로 구현될 수 있음을 이해할 수 있을 것이다. 그러므로 개시된 실시예들은 한정적인 관점이 아니라 설명적인 관점에서 고려되어야 한다. 본 발명의 범위는 전술한 설명이 아니라 특허청구범위에 나타나 있으며, 그와 동등한 범위 내에 있는 모든 차이점은 본 발명에 포함된 것으로 해석되어야 할 것이다.The present invention has been described with reference to the preferred embodiments. It will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims. Therefore, the disclosed embodiments should be considered in an illustrative rather than a restrictive sense. The scope of the present invention is defined by the appended claims rather than by the foregoing description, and all differences within the scope of equivalents thereof should be construed as being included in the present invention.

Claims (6)

  1. 2,3-부탄다이올(2,3-butanediol)을 유효성분으로 포함하는 식물바이러스 방제용 조성물.A composition for controlling a plant virus comprising 2,3-butanediol as an active ingredient.
  2. 제1항에 있어서, 상기 식물바이러스는 오이 모자이크 바이러스 (cucumber mosaic virus, CMV), 담배 모자이크 바이러스 (tobacco mosaic virus, TMV), 고추얼룩무늬바이러스 (Pepper mottle virus, PepMoV), 토마토반점시듦바이러스 (Tomato Spotted Wilt Virus, TSWV) 및 토마토황화잎말림바이러스 (Tomato yellow leaf curl virus, TYLCV)로 이루어진 군에서 선택되는 하나 이상인 것인 식물바이러스 방제용 조성물.The plant virus according to claim 1, wherein the plant virus is selected from the group consisting of cucumber mosaic virus (CMV), tobacco mosaic virus (TMV), pepper mottle virus (PepMoV), tomato spotting virus Spotted Wilt Virus (TSWV), and Tomato yellow leaf curl virus (TYLCV).
  3. 제1항에 있어서, 상기 2,3-부탄다이올은 조성물 전체 중량 대비 0.1% 내지 50%로 포함되는 것인 식물바이러스 방제용 조성물.The composition according to claim 1, wherein the 2,3-butanediol is contained in an amount of 0.1% to 50% based on the total weight of the composition.
  4. 제1항에 있어서, 상기 조성물은 액제, 입제, 분제, 유제, 오일제, 수화제 및 도포제로 이루어진 군에서 선택되는 형태로 제형화되는 것인 식물바이러스 방제용 조성물.The composition according to claim 1, wherein the composition is formulated into a form selected from the group consisting of a liquid agent, a granule, a powder, an oil, an oil, a wetting agent and a coating agent.
  5. 제1항 내지 제4항 중 어느 한 항의 조성물을 포함하는 식물바이러스 방제용 농약 조성물.A pesticidal composition for controlling plant viruses, comprising the composition of any one of claims 1 to 4.
  6. 제1항 내지 제4항 중 어느 한 항의 조성물을 식물체 또는 토양에 처리하는 단계를 포함하는 식물바이러스의 방제 방법.A method for controlling plant viruses, comprising the step of treating a plant or soil with the composition of any one of claims 1 to 4.
PCT/KR2018/010954 2017-09-18 2018-09-18 Composition for controlling plant viruses, and use thereof WO2019054840A2 (en)

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