WO2019051982A1 - Method for preparing microbial pattern - Google Patents

Method for preparing microbial pattern Download PDF

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Publication number
WO2019051982A1
WO2019051982A1 PCT/CN2017/110647 CN2017110647W WO2019051982A1 WO 2019051982 A1 WO2019051982 A1 WO 2019051982A1 CN 2017110647 W CN2017110647 W CN 2017110647W WO 2019051982 A1 WO2019051982 A1 WO 2019051982A1
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WO
WIPO (PCT)
Prior art keywords
medium
pattern
microorganisms
colors
same
Prior art date
Application number
PCT/CN2017/110647
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French (fr)
Chinese (zh)
Inventor
王航
王大平
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如皋福大工程技术研究院有限公司
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Publication of WO2019051982A1 publication Critical patent/WO2019051982A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41M—PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES; COLOUR PRINTING
    • B41M5/00—Duplicating or marking methods; Sheet materials for use therein
    • B41M5/0023—Digital printing methods characterised by the inks used
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41M—PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES; COLOUR PRINTING
    • B41M5/00—Duplicating or marking methods; Sheet materials for use therein
    • B41M5/0041—Digital printing on surfaces other than ordinary paper
    • B41M5/0058—Digital printing on surfaces other than ordinary paper on metals and oxidised metal surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
    • B41M—PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES; COLOUR PRINTING
    • B41M5/00—Duplicating or marking methods; Sheet materials for use therein
    • B41M5/0041—Digital printing on surfaces other than ordinary paper
    • B41M5/007—Digital printing on surfaces other than ordinary paper on glass, ceramic, tiles, concrete, stones, etc.

Definitions

  • the invention belongs to the field of microbial art, and in particular to a method for preparing a microbial pattern.
  • the prior art in order to obtain a beautiful pattern, it is basically prepared by painting or textile and embroidery, and when using the painting technique, a pigment is used, and the pigment basically includes two types of organic pigments and inorganic pigments, but such pigments In the production process, there is a certain environmental damage.
  • a method for preparing a microbial pattern comprising the steps of:
  • the second medium is uniformly coated on the plate under aseptic conditions, and after the second medium is solidified, it is placed in an inkjet printer, and the bacterial suspension is printed on the plate according to a pre-arranged design pattern in the computer.
  • the transparent aseptic box is placed in a constant temperature incubator, cultured at a set temperature, and the culture results are periodically observed. After the desired pattern is removed from the transparent sterile box, it is fixed with formalin and then sealed. .
  • the culture conditions were the same and the growth rates were similar.
  • Microorganisms of all three colors are microorganisms that are harmless to humans.
  • the first medium and the second medium are the same and both are agar medium.
  • the sheet material is a glass sheet, an iron sheet, a sheet, a cardboard or a plastic sheet.
  • An image capturing device is disposed in the constant temperature incubator, and the camera device is connected to the computer, and the camera captures an instant image and compares with a preset pattern in the computer, and compares the instant image with the preset pattern.
  • the transparent sterile box is taken out from the incubator.
  • the technical solution is to obtain a preset pattern by the growth of colored microorganisms and printing by an inkjet printer, and in the process, there is no damage to the environment, and the types of patterns are various.
  • the application provides a method for preparing a microbial pattern
  • the spores of the microorganisms of the three colors were collected to prepare a bacterial suspension; the above three bacterial suspensions were injected into the nozzle of a color inkjet printer.
  • the second medium is uniformly applied to the plate under aseptic conditions, and the second medium is placed after solidification.
  • the bacterial suspension is printed onto the second medium of the plate according to a pre-programmed pattern in the computer, and placed in a transparent sterile box;
  • the transparent aseptic box is placed in a constant temperature incubator, cultured at a set temperature, and the culture results are periodically observed. After the desired pattern is removed from the transparent sterile box, it is fixed with formalin and then sealed. .
  • the culture conditions were the same and the growth rates were similar.
  • Microorganisms of all three colors are microorganisms that are harmless to humans.
  • the first medium and the second medium are the same and both are agar medium. In other embodiments of the application, the first medium and the second medium may be different.
  • the sheet material is a glass sheet, an iron sheet, a sheet, a cardboard or a plastic sheet.
  • An image capturing device is disposed in the constant temperature incubator, and the camera device is connected to the computer, and the camera captures an instant image and compares with a preset pattern in the computer, and compares the instant image with the preset pattern.
  • the transparent sterile box is taken out from the incubator.

Abstract

Disclosed is a method for preparing a microbial pattern. The method comprises the steps of: selecting a microorganism with a red colony, a microorganism with a green colony and a microorganism with a blue colony, and cultivating the microorganisms of the above-mentioned three colors in a first medium, respectively; collecting spores of the microorganisms of the three colors to prepare a bacterial suspension; infusing the bacterial suspension in the nozzle of a color ink jet printer; and coating a second medium evenly on a plate under sterile conditions, placing the plate in the ink jet printer after the second medium has solidified, printing the bacterial suspension on the second medium of the plate according to a pre-arranged and designed pattern in a computer, placing same in a transparent sterile box and cultivating same in a constant-temperature incubator, regularly observing culture results, and when the desired pattern is formed, removing same from the transparent sterile box, fixing same with formalin and then hermetically packaging same.

Description

一种微生物图案制备方法Microbial pattern preparation method 技术领域Technical field
本发明属于微生物艺术领域，特别是指一种微生物图案制备方法。The invention belongs to the field of microbial art, and in particular to a method for preparing a microbial pattern.
背景技术Background technique
现技术中，为了得到精美的图案，基本上是通过绘画或纺织及刺绣等制备的，使用绘画技术时，使用的是颜料，现颜料基本包括有机颜料和无机颜料两大类，但是，这样颜料的生产过程中，均存在一定的环境破坏。In the prior art, in order to obtain a beautiful pattern, it is basically prepared by painting or textile and embroidery, and when using the painting technique, a pigment is used, and the pigment basically includes two types of organic pigments and inorganic pigments, but such pigments In the production process, there is a certain environmental damage.
使用纺织或刺绣等制备彩色图案时，也需要对纤维进行染色等工序，而在染色工序中，需要使用颜色、加热等工序，并产生大量的废水，均对环境产生破坏的影响。When a color pattern is prepared by using textiles or embroidery, it is also necessary to dye the fibers, and in the dyeing process, it is necessary to use processes such as color and heating, and a large amount of waste water is generated, which has an effect on the environment.
在自然界中，存在五颜六色的微生物菌落，若能够以此为颜料绘制出彩色图案，不仅不需要破坏环境，而且也可以根据不同的需要设计不同的图案，这是现有任何文献没有提及的。In nature, there are colorful microbial colonies. If you can draw a color pattern with this pigment, you don't need to damage the environment, but you can also design different patterns according to different needs, which is not mentioned in any existing literature.
发明内容Summary of the invention
本发明的目的是提供一种微生物图案制备方法，以解决现夺的彩色图案的制备均对环境产生破坏的影响的问题。It is an object of the present invention to provide a method for preparing a microbial pattern to solve the problem that the preparation of a current color pattern has an effect on the environment.
本发明是通过以下技术方案实现的：The invention is achieved by the following technical solutions:
一种微生物图案制备方法，包括以下步骤：A method for preparing a microbial pattern, comprising the steps of:
颜料的选择Pigment selection
选择菌落呈红颜色的微生物、绿颜色的微生物及蓝颜色的微生物，将上述三种颜色的微生物分别在第一培养基内培养；Selecting a microorganism having a red color of a colony, a microorganism of a green color, and a microorganism of a blue color, and cultivating the microorganisms of the above three colors in the first medium;
采集这三种颜色的微生物的孢子制备成菌悬液；将上述三种菌悬液注入到彩色喷墨打印机的喷头中；Collecting the spores of the microorganisms of the three colors into a bacterial suspension; injecting the above three bacterial suspensions into the nozzle of the color inkjet printer;
打印介质的制备Preparation of print media
在无菌条件下将第二培养基均匀涂布于板材上，待第二培养基凝固后放入喷墨打印机中，按电脑中预先编排设计的图案将菌悬液打印到所述板材的第二 培养基上，并置于透明无菌盒中；The second medium is uniformly coated on the plate under aseptic conditions, and after the second medium is solidified, it is placed in an inkjet printer, and the bacterial suspension is printed on the plate according to a pre-arranged design pattern in the computer. Two On the medium and placed in a transparent sterile box;
培养to cultivate
将透明无菌盒置于恒温培养箱中，在设定温度下培养，定期观察培养结果，当第成所需要的图案后从透明无菌盒中取出，用福尔马林固定，然后密闭封装。The transparent aseptic box is placed in a constant temperature incubator, cultured at a set temperature, and the culture results are periodically observed. After the desired pattern is removed from the transparent sterile box, it is fixed with formalin and then sealed. .
三种颜色的微生物分别在第一培养基内进行培养时，培养条件相同，生长速度相近。When the microorganisms of the three colors were cultured in the first medium, the culture conditions were the same and the growth rates were similar.
三种颜色的微生物均为对人无害的微生物。Microorganisms of all three colors are microorganisms that are harmless to humans.
所述第一培养基和第二培养基相同，均为琼脂培养基。The first medium and the second medium are the same and both are agar medium.
所述板材为玻璃板、铁板、本板、纸板或塑料板。The sheet material is a glass sheet, an iron sheet, a sheet, a cardboard or a plastic sheet.
在所述恒温培养箱内设置有摄像装置，所述摄像装置与所述电脑连接，摄像装置拍摄即时影像与电脑中的预设图案进行比对，当即时影像与所述预设图案经过比对，相同或相近似时，将透明无菌盒从所述恒温培养箱内取出。An image capturing device is disposed in the constant temperature incubator, and the camera device is connected to the computer, and the camera captures an instant image and compares with a preset pattern in the computer, and compares the instant image with the preset pattern. When the same or similar, the transparent sterile box is taken out from the incubator.
本发明的有益效果是：The beneficial effects of the invention are:
本技术方案是通过带颜色的微生物的生长，并通过喷墨打印机的打印，得到预设的图案，而这一过程中，不会对环境产生任何的破坏，并且图案的种类更多样。The technical solution is to obtain a preset pattern by the growth of colored microorganisms and printing by an inkjet printer, and in the process, there is no damage to the environment, and the types of patterns are various.
具体实施方式Detailed ways
以下通过实施例来详细说明本发明的技术方案，以下的实施例仅是示例性的，仅能用来解释和说明本发明的技术方案，而不能解释为是对本发明技术方案的限制。The following is a detailed description of the technical solutions of the present invention by way of examples, and the following embodiments are merely illustrative and are not to be construed as limiting the technical solutions of the present invention.
本申请提供一种微生物图案制备方法，The application provides a method for preparing a microbial pattern,
颜料的选择Pigment selection
选择菌落呈红颜色的微生物、绿颜色的微生物及蓝颜色的微生物，将上述三种颜色的微生物分别在第一培养基内培养；Selecting a microorganism having a red color of a colony, a microorganism of a green color, and a microorganism of a blue color, and cultivating the microorganisms of the above three colors in the first medium;
采集这三种颜色的微生物的孢子制备成菌悬液；将上述三种菌悬液注入到彩色喷墨打印机的喷头中。The spores of the microorganisms of the three colors were collected to prepare a bacterial suspension; the above three bacterial suspensions were injected into the nozzle of a color inkjet printer.
打印介质的制备Preparation of print media
在无菌条件下将第二培养基均匀涂布于板材上，待第二培养基凝固后放入 喷墨打印机中，按电脑中预先编排设计的图案将菌悬液打印到所述板材的第二培养基上，并置于透明无菌盒中；The second medium is uniformly applied to the plate under aseptic conditions, and the second medium is placed after solidification. In an inkjet printer, the bacterial suspension is printed onto the second medium of the plate according to a pre-programmed pattern in the computer, and placed in a transparent sterile box;
培养to cultivate
将透明无菌盒置于恒温培养箱中，在设定温度下培养，定期观察培养结果，当第成所需要的图案后从透明无菌盒中取出，用福尔马林固定，然后密闭封装。The transparent aseptic box is placed in a constant temperature incubator, cultured at a set temperature, and the culture results are periodically observed. After the desired pattern is removed from the transparent sterile box, it is fixed with formalin and then sealed. .
三种颜色的微生物分别在第一培养基内进行培养时，培养条件相同，生长速度相近。When the microorganisms of the three colors were cultured in the first medium, the culture conditions were the same and the growth rates were similar.
三种颜色的微生物均为对人无害的微生物。Microorganisms of all three colors are microorganisms that are harmless to humans.
所述第一培养基和第二培养基相同，均为琼脂培养基。在本申请的其它实施例中，第一培养基和第二培养基可以不同。The first medium and the second medium are the same and both are agar medium. In other embodiments of the application, the first medium and the second medium may be different.
所述板材为玻璃板、铁板、本板、纸板或塑料板。The sheet material is a glass sheet, an iron sheet, a sheet, a cardboard or a plastic sheet.
在所述恒温培养箱内设置有摄像装置，所述摄像装置与所述电脑连接，摄像装置拍摄即时影像与电脑中的预设图案进行比对，当即时影像与所述预设图案经过比对，相同或相近似时，将透明无菌盒从所述恒温培养箱内取出。An image capturing device is disposed in the constant temperature incubator, and the camera device is connected to the computer, and the camera captures an instant image and compares with a preset pattern in the computer, and compares the instant image with the preset pattern. When the same or similar, the transparent sterile box is taken out from the incubator.
尽管已经示出和描述了本发明的实施例，对于本领域的普通技术人员而言，可以理解在不脱离本发明的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变形，本发明的范围由所附权利要求极其等同限定。 While the embodiments of the present invention have been shown and described, it will be understood by those skilled in the art And the scope of the invention is defined by the appended claims.

Claims (6)

  1. 一种微生物图案制备方法，其特征在于，包括以下步骤：A method for preparing a microbial pattern, comprising the steps of:
    颜料的选择Pigment selection
    选择菌落呈红颜色的微生物、绿颜色的微生物及蓝颜色的微生物，将上述三种颜色的微生物分别在第一培养基内培养；Selecting a microorganism having a red color of a colony, a microorganism of a green color, and a microorganism of a blue color, and cultivating the microorganisms of the above three colors in the first medium;
    采集这三种颜色的微生物的孢子制备成菌悬液；将上述三种菌悬液注入到彩色喷墨打印机的喷头中；Collecting the spores of the microorganisms of the three colors into a bacterial suspension; injecting the above three bacterial suspensions into the nozzle of the color inkjet printer;
    打印介质的制备Preparation of print media
    在无菌条件下将第二培养基均匀涂布于板材上，待第二培养基凝固后放入喷墨打印机中，按电脑中预先编排设计的图案将菌悬液打印到所述板材的第二培养基上，并置于透明无菌盒中；The second medium is uniformly coated on the plate under aseptic conditions, and after the second medium is solidified, it is placed in an inkjet printer, and the bacterial suspension is printed on the plate according to a pre-arranged design pattern in the computer. On the medium, and placed in a transparent sterile box;
    培养to cultivate
    将透明无菌盒置于恒温培养箱中，在设定温度下培养，定期观察培养结果，当第成所需要的图案后从透明无菌盒中取出，用福尔马林固定，然后密闭封装。The transparent aseptic box is placed in a constant temperature incubator, cultured at a set temperature, and the culture results are periodically observed. After the desired pattern is removed from the transparent sterile box, it is fixed with formalin and then sealed. .
  2. 根据权利要求1所述的微生物图案制备方法，其特征在于，三种颜色的微生物分别在第一培养基内进行培养时，培养条件相同，生长速度相近。The method for preparing a microbial pattern according to claim 1, wherein when the microorganisms of the three colors are cultured in the first medium, the culture conditions are the same and the growth rates are similar.
  3. 根据权利要求1所述的微生物图案制备方法，其特征在于，三种颜色的微生物均为对人无害的微生物。The microbial pattern preparation method according to claim 1, wherein the microorganisms of the three colors are microorganisms which are harmless to humans.
  4. 根据权利要求1所述的微生物图案制备方法，其特征在于，所述第一培养基和第二培养基相同，均为琼脂培养基。The method for preparing a microbial pattern according to claim 1, wherein the first medium and the second medium are the same, both of which are agar medium.
  5. 根据权利要求1所述的微生物图案制备方法，其特征在于，所述板材为玻璃板、铁板、本板、纸板或塑料板。The microbial pattern preparation method according to claim 1, wherein the plate material is a glass plate, an iron plate, a plate, a cardboard or a plastic plate.
  6. 根据权利要求1所述的微生物图案制备方法，其特征在于，在所述恒温培养箱内设置有摄像装置，所述摄像装置与所述电脑连接，摄像装置拍摄即时影像与电脑中的预设图案进行比对，当即时影像与所述预设图案经过比对，相同或相近似时，将透明无菌盒从所述恒温培养箱内取出。 The microbial pattern preparation method according to claim 1, wherein an imaging device is disposed in the constant temperature incubator, the imaging device is connected to the computer, and the camera device captures a preset image in an instant image and a computer. For comparison, when the instant image is compared with the preset pattern, the same or similar, the transparent sterile box is taken out from the constant temperature incubator.
PCT/CN2017/110647 2017-09-15 2017-11-13 Method for preparing microbial pattern WO2019051982A1 (en)

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