CN107446821A - A kind of microorganism pattern preparation method - Google Patents
A kind of microorganism pattern preparation method Download PDFInfo
- Publication number
- CN107446821A CN107446821A CN201710834351.6A CN201710834351A CN107446821A CN 107446821 A CN107446821 A CN 107446821A CN 201710834351 A CN201710834351 A CN 201710834351A CN 107446821 A CN107446821 A CN 107446821A
- Authority
- CN
- China
- Prior art keywords
- microorganism
- culture medium
- pattern
- sterility
- transparent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
- B41M—PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES; COLOUR PRINTING
- B41M5/00—Duplicating or marking methods; Sheet materials for use therein
- B41M5/0023—Digital printing methods characterised by the inks used
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
- B41M—PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES; COLOUR PRINTING
- B41M5/00—Duplicating or marking methods; Sheet materials for use therein
- B41M5/0041—Digital printing on surfaces other than ordinary paper
- B41M5/0058—Digital printing on surfaces other than ordinary paper on metals and oxidised metal surfaces
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B41—PRINTING; LINING MACHINES; TYPEWRITERS; STAMPS
- B41M—PRINTING, DUPLICATING, MARKING, OR COPYING PROCESSES; COLOUR PRINTING
- B41M5/00—Duplicating or marking methods; Sheet materials for use therein
- B41M5/0041—Digital printing on surfaces other than ordinary paper
- B41M5/007—Digital printing on surfaces other than ordinary paper on glass, ceramic, tiles, concrete, stones, etc.
Abstract
The present invention relates to a kind of microorganism pattern preparation method, microorganism, the microorganism of green color and the microorganism of blue color for selecting bacterium colony to be in red color, the microorganism of above-mentioned three kinds of colors is cultivated in the first culture medium respectively；The spore for gathering the microorganism of these three colors is prepared into bacteria suspension；It is injected into the shower nozzle of color inkjet printer；Aseptically the second culture medium is spread evenly across on sheet material, it is put into after the second culture medium solidifying in ink-jet printer, bacteria suspension is printed on the second culture medium of the sheet material by the pattern of advance design in computer, it is placed in cultivating in constant incubator in transparent sterility, routine observation cultivation results, take out, fixed with formalin, then enclosed package from transparent sterility after pattern required for the one-tenth.By the growth of colored microorganism, and by the printing of ink-jet printer, default pattern is obtained, and during this, any destruction will not be produced to environment.
Description
Technical field
The invention belongs to microorganism world of art, particularly relates to a kind of microorganism pattern preparation method.
Background technology
In existing technology, in order to obtain the pattern of fineness, substantially pass through the preparation such as paint or weave and embroider, use
During drawing techniques, pigment is used, existing pigment includes organic pigment and the major class of inorganic pigment two, still, such pigment substantially
Production process in, in the presence of certain environmental disruption.
When preparing multicolour pattern using weaving or embroidery etc., it is also desirable to fiber is carried out the process such as to dye, and in dyer
In sequence, it is necessary to using processes such as color, heating, and substantial amounts of waste water is produced, the influence destroyed is produced to environment.
In nature, motley microbe colony be present, if multicolour pattern can be drawn out as pigment, no
Welding is not only needed, and can also need to design different patterns according to different, this is that existing any document does not have
Refer to.
The content of the invention
It is an object of the invention to provide a kind of microorganism pattern preparation method, preparing for the multicolour pattern now taken by force with solution are equal
The problem of influence destroyed is produced to environment.
The present invention is achieved by the following technical solutions：
A kind of microorganism pattern preparation method, comprises the following steps：
The selection of pigment
Microorganism, the microorganism of green color and the microorganism of blue color that bacterium colony is in red color are selected, by above-mentioned three kinds of face
The microorganism of color is cultivated in the first culture medium respectively；
The spore for gathering the microorganism of these three colors is prepared into bacteria suspension；Above-mentioned three kinds of bacteria suspensions are injected into colored spray
In the shower nozzle of black printer；
The preparation of print media
Aseptically the second culture medium is spread evenly across on sheet material, being put into ink-jet after the second culture medium solidifying beats
In print machine, bacteria suspension is printed on the second culture medium of the sheet material by the pattern of advance design in computer, is placed in
In transparent sterility；
Culture
Transparent sterility is placed in constant incubator, cultivated at a set temperature, routine observation cultivation results, when the one-tenth
Take out from transparent sterility after required pattern, fixed with formalin, then enclosed package.
When the microorganism of three kinds of colors is cultivated in the first culture medium respectively, condition of culture is identical, speed of growth phase
Closely.
The microorganism of three kinds of colors is microorganism harmless to humans.
First culture medium and the second culture medium are identical, are agar medium.
The sheet material is glass plate, iron plate, this plate, cardboard or plastic plate.
Camera device is provided with the constant incubator, the camera device is connected with the computer, camera device
Shooting instant video is compared with the predetermined pattern in computer, identical when instant video with the predetermined pattern by comparing
Or when close, transparent sterility is taken out out of described constant incubator.
The beneficial effects of the invention are as follows：
The technical program is by the growth of colored microorganism, and by the printing of ink-jet printer, is preset
Pattern, and during this, any destruction will not be produced to environment, and the species of pattern is more various.
Embodiment
Describe technical scheme in detail by the following examples, following embodiment be only it is exemplary, only
Explanation and illustration technical scheme can be used for, and be not to be construed as the limitation to technical solution of the present invention.
The application provides a kind of microorganism pattern preparation method,
The selection of pigment
Microorganism, the microorganism of green color and the microorganism of blue color that bacterium colony is in red color are selected, by above-mentioned three kinds of face
The microorganism of color is cultivated in the first culture medium respectively；
The spore for gathering the microorganism of these three colors is prepared into bacteria suspension；Above-mentioned three kinds of bacteria suspensions are injected into colored spray
In the shower nozzle of black printer.
The preparation of print media
Aseptically the second culture medium is spread evenly across on sheet material, being put into ink-jet after the second culture medium solidifying beats
In print machine, bacteria suspension is printed on the second culture medium of the sheet material by the pattern of advance design in computer, is placed in
In transparent sterility；
Culture
Transparent sterility is placed in constant incubator, cultivated at a set temperature, routine observation cultivation results, when the one-tenth
Take out from transparent sterility after required pattern, fixed with formalin, then enclosed package.
When the microorganism of three kinds of colors is cultivated in the first culture medium respectively, condition of culture is identical, speed of growth phase
Closely.
The microorganism of three kinds of colors is microorganism harmless to humans.
First culture medium and the second culture medium are identical, are agar medium.In the other embodiments of the application,
First culture medium and the second culture medium can be different.
The sheet material is glass plate, iron plate, this plate, cardboard or plastic plate.
Camera device is provided with the constant incubator, the camera device is connected with the computer, camera device
Shooting instant video is compared with the predetermined pattern in computer, identical when instant video with the predetermined pattern by comparing
Or when close, transparent sterility is taken out out of described constant incubator.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And deformation, the scope of the present invention are extremely equally limited by appended claims.
Claims (6)
1. a kind of microorganism pattern preparation method, it is characterised in that comprise the following steps：
The selection of pigment
Microorganism, the microorganism of green color and the microorganism of blue color that bacterium colony is in red color are selected, by above-mentioned three kinds of colors
Microorganism is cultivated in the first culture medium respectively；
The spore for gathering the microorganism of these three colors is prepared into bacteria suspension；Above-mentioned three kinds of bacteria suspensions are injected into color jet-ink to beat
In the shower nozzle of print machine；
The preparation of print media
Aseptically the second culture medium is spread evenly across on sheet material, ink-jet printer is put into after the second culture medium solidifying
In, bacteria suspension is printed on the second culture medium of the sheet material by the pattern of advance design in computer, is placed in transparent
In sterility；
Culture
Transparent sterility is placed in constant incubator, cultivated at a set temperature, routine observation cultivation results, when needed for the one-tenth
Take out from transparent sterility after the pattern wanted, fixed with formalin, then enclosed package.
2. microorganism pattern preparation method according to claim 1, it is characterised in that the microorganism of three kinds of colors exists respectively
When being cultivated in the first culture medium, condition of culture is identical, and the speed of growth is close.
3. microorganism pattern preparation method according to claim 1, it is characterised in that the microorganism of three kinds of colors is pair
The harmless microorganism of people.
4. microorganism pattern preparation method according to claim 1, it is characterised in that first culture medium and the second training
It is identical to support base, is agar medium.
5. microorganism pattern preparation method according to claim 1, it is characterised in that the sheet material be glass plate, iron plate,
This plate, cardboard or plastic plate.
6. microorganism pattern preparation method according to claim 1, it is characterised in that set in the constant incubator
There is camera device, the camera device is connected with the computer, camera device shooting instant video and the predetermined pattern in computer
Be compared, when instant video and the predetermined pattern are by comparing, it is same or like like when, by transparent sterility from the perseverance
Taken out in warm incubator.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710834351.6A CN107446821A (en) | 2017-09-15 | 2017-09-15 | A kind of microorganism pattern preparation method |
| PCT/CN2017/110647 WO2019051982A1 (en) | 2017-09-15 | 2017-11-13 | Method for preparing microbial pattern |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710834351.6A CN107446821A (en) | 2017-09-15 | 2017-09-15 | A kind of microorganism pattern preparation method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107446821A true CN107446821A (en) | 2017-12-08 |
Family
ID=60496588
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710834351.6A Pending CN107446821A (en) | 2017-09-15 | 2017-09-15 | A kind of microorganism pattern preparation method |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN107446821A (en) |
| WO (1) | WO2019051982A1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112277521A (en) * | 2020-10-27 | 2021-01-29 | 安庆师范大学 | Method for making creative microorganism specimen picture with stable appearance character |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008026104A2 (en) * | 2006-08-31 | 2008-03-06 | Kimberly-Clark Worldwide, Inc. | Array for rapid detection of a microorganism |
| CN101456294A (en) * | 2009-01-08 | 2009-06-17 | 北京美科艺数码科技发展有限公司 | Arrangement method of different color ink nozzle for colorful ink jet printer |
| CN102251018A (en) * | 2011-05-20 | 2011-11-23 | 浙江大学 | Method for measuring minimal inhibitory concentration (MIC) of antibiotics based on inkjet printing principle |
| CN102297931A (en) * | 2011-05-20 | 2011-12-28 | 浙江大学 | Antibiotic titer determination method based on ink-jet printing principle |
| WO2013158740A1 (en) * | 2012-04-18 | 2013-10-24 | Biofire Diagnostics, Inc. | Microspotting device |
-
2017
- 2017-09-15 CN CN201710834351.6A patent/CN107446821A/en active Pending
- 2017-11-13 WO PCT/CN2017/110647 patent/WO2019051982A1/en active Application Filing
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008026104A2 (en) * | 2006-08-31 | 2008-03-06 | Kimberly-Clark Worldwide, Inc. | Array for rapid detection of a microorganism |
| CN101456294A (en) * | 2009-01-08 | 2009-06-17 | 北京美科艺数码科技发展有限公司 | Arrangement method of different color ink nozzle for colorful ink jet printer |
| CN102251018A (en) * | 2011-05-20 | 2011-11-23 | 浙江大学 | Method for measuring minimal inhibitory concentration (MIC) of antibiotics based on inkjet printing principle |
| CN102297931A (en) * | 2011-05-20 | 2011-12-28 | 浙江大学 | Antibiotic titer determination method based on ink-jet printing principle |
| WO2013158740A1 (en) * | 2012-04-18 | 2013-10-24 | Biofire Diagnostics, Inc. | Microspotting device |
Non-Patent Citations (1)
| Title |
|---|
| W.CRIS WILSON, ET AL.: "Cell and Organ Printing 1: Protein and Cell Printers.", 《THE ANATOMICAL RECORD PART A》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112277521A (en) * | 2020-10-27 | 2021-01-29 | 安庆师范大学 | Method for making creative microorganism specimen picture with stable appearance character |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019051982A1 (en) | 2019-03-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4314813A (en) | Flock transfer sheet and flock transfer printing process | |
| JP5548886B2 (en) | Laser marking method | |
| CN108032620B (en) | Utilize the duplex inkjet printing method and textile of the textile of Numerical Control Printer | |
| CN202944018U (en) | Conduction band type digital printing machine for printing patterns on cross-stitch fabric | |
| ATE174643T1 (en) | INKJET TEXTILE PRINTING PROCESS USING DISPERSION DYES AND PRINTED TEXTILES AVAILABLE IN THIS WAY | |
| US20020155224A1 (en) | Direct dye inks and a method of making direct dye inks | |
| CN105696380B (en) | A kind of method of recognizable ink-jet printed fabric pretreatment uniformity | |
| NO149317B (en) | HEAT-SENSITIVE PRINTING TOP FOR DECORATION OR MARKING OF TEXTILES AND OTHER ABSORBING RECEIVER SURFACES | |
| ATE367473T1 (en) | PRINTING PROCESS AND PRINTS PRODUCED THEREFROM AND ARTICLES PRODUCED USING THIS PROCESS | |
| KR950003014A (en) | Printing methods and printed matter and processed products obtained therefrom | |
| EP2481602A1 (en) | Process for printing on surfaces of appliances | |
| CN107446821A (en) | A kind of microorganism pattern preparation method | |
| US5353706A (en) | Method of printing on natural fiber fabrics using a resin-free varnish | |
| CN205380985U (en) | Fabric transfer printing device | |
| CN106988044A (en) | One kind embroidery dyeing and printing process | |
| CN102433783A (en) | Printing process for cloth | |
| KR102040584B1 (en) | Composition for thermal transfer paper, transfer paper using the same and method for transfer printing thereof | |
| EP3564439A1 (en) | Paper-free transfer printing method | |
| CN109835078A (en) | A method of avoid the thermal transfer printing on embroidered pattern from misplacing | |
| CN1005279B (en) | Colored ink for transferring and pattern-printing method | |
| US6820555B2 (en) | Printing process and printing apparatus | |
| CN206266854U (en) | One kind is dyed cloth nozzle | |
| CN206718695U (en) | A kind of high warm transfer fabric printing system | |
| CN101113256A (en) | Ink for silk brocade cloth thermal transfer printing | |
| CN111298284A (en) | Manufacturing method of printed non-woven fabric electrode slice |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171208 |
|
| WD01 | Invention patent application deemed withdrawn after publication |