WO2019050101A3 - 3차원 세포배양 용기 - Google Patents

3차원 세포배양 용기 Download PDF

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Publication number
WO2019050101A3
WO2019050101A3 PCT/KR2017/015160 KR2017015160W WO2019050101A3 WO 2019050101 A3 WO2019050101 A3 WO 2019050101A3 KR 2017015160 W KR2017015160 W KR 2017015160W WO 2019050101 A3 WO2019050101 A3 WO 2019050101A3
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WIPO (PCT)
Prior art keywords
sample
cell culture
culture container
cell
upper plate
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PCT/KR2017/015160
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English (en)
French (fr)
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WO2019050101A9 (ko
WO2019050101A2 (ko
Inventor
김정아
김영혜
유영준
정석
양지훈
Original Assignee
한국기초과학지원연구원
고려대학교산학협력단
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by 한국기초과학지원연구원, 고려대학교산학협력단 filed Critical 한국기초과학지원연구원
Priority to CN201780094660.2A priority Critical patent/CN111164200A/zh
Priority to US16/645,539 priority patent/US20200283711A1/en
Priority to EP17924352.2A priority patent/EP3680321A4/en
Publication of WO2019050101A2 publication Critical patent/WO2019050101A2/ko
Publication of WO2019050101A9 publication Critical patent/WO2019050101A9/ko
Publication of WO2019050101A3 publication Critical patent/WO2019050101A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0607—Non-embryonic pluripotent stem cells, e.g. MASC
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2535/00—Supports or coatings for cell culture characterised by topography
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Cell Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

본 발명은 3차원 세포배양 용기에 관한 것이다. 본 발명의 세포배양 용기는 하판(120); 상기 하판의 상측에 구비되는 둘 이상의 기둥(110)으로 이루어진 배리어부; 상기 배리어부의 개방된 단부를 마감하는 상판(130); 상기 상판의 일측에 구비된 하나 이상의 세포시료 주입 가이드부(220);를 포함하여 구성될 수 있다. 이러한 구성에 의한 본 발명은 세포를 포함하는 시료를 가이드부에 의지하여 안정적으로 정확한 위치에 주입할 수 있으며, 상판과 다수개의 기둥이 적정량의 시료를 손쉽고 균일하게 주입할 수 있도록 돕는 역할을 함으로써 실험자의 작업을 용이하게 하는 한편, 일정 간격의 기둥부가 젤 형태의 시료가 외부로 빠져나가지 못하도록 하는 배리어 역할을 하고 외부 셀에 있는 배지와 물질교환을 할 수 있도록 공간을 개방하는 역할을 함으로써 안정적인 세포배양을 할 수 있다.
PCT/KR2017/015160 2017-09-07 2017-12-20 3차원 세포배양 용기 WO2019050101A2 (ko)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201780094660.2A CN111164200A (zh) 2017-09-07 2017-12-20 三维细胞培养容器
US16/645,539 US20200283711A1 (en) 2017-09-07 2017-12-20 Three-dimensional cell culture container
EP17924352.2A EP3680321A4 (en) 2017-09-07 2017-12-20 THREE-DIMENSIONAL CELL CULTURE CONTAINER

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0114829 2017-09-07
KR1020170114829A KR101847044B1 (ko) 2017-09-07 2017-09-07 3차원 세포배양 용기

Publications (3)

Publication Number Publication Date
WO2019050101A2 WO2019050101A2 (ko) 2019-03-14
WO2019050101A9 WO2019050101A9 (ko) 2019-04-11
WO2019050101A3 true WO2019050101A3 (ko) 2019-05-23

Family

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PCT/KR2017/015160 WO2019050101A2 (ko) 2017-09-07 2017-12-20 3차원 세포배양 용기

Country Status (5)

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US (1) US20200283711A1 (ko)
EP (1) EP3680321A4 (ko)
KR (1) KR101847044B1 (ko)
CN (1) CN111164200A (ko)
WO (1) WO2019050101A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102233285B1 (ko) 2018-11-08 2021-03-29 한국화학연구원 미세유체소자를 이용한 3차원 세포배양

Citations (4)

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Publication number Priority date Publication date Assignee Title
KR20130120032A (ko) * 2012-04-25 2013-11-04 포항공과대학교 산학협력단 세포 배양 및 응용연구를 위한 미세 관류 소자 및 그 제조방법
KR20140050345A (ko) * 2012-10-19 2014-04-29 한국생명공학연구원 이중구조 세포배양용기
US20140255970A1 (en) * 2013-03-08 2014-09-11 Cfd Research Corporation Synthetic microfluidic systems for hypoxia
KR20170074503A (ko) * 2015-12-22 2017-06-30 고려대학교 산학협력단 세포 배양 어세이를 이용한 약물 평가 방법

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CN102803466B (zh) * 2009-06-23 2014-11-05 株式会社日立制作所 培养器材、培养片材及细胞培养方法
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Patent Citations (4)

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KR20130120032A (ko) * 2012-04-25 2013-11-04 포항공과대학교 산학협력단 세포 배양 및 응용연구를 위한 미세 관류 소자 및 그 제조방법
KR20140050345A (ko) * 2012-10-19 2014-04-29 한국생명공학연구원 이중구조 세포배양용기
US20140255970A1 (en) * 2013-03-08 2014-09-11 Cfd Research Corporation Synthetic microfluidic systems for hypoxia
KR20170074503A (ko) * 2015-12-22 2017-06-30 고려대학교 산학협력단 세포 배양 어세이를 이용한 약물 평가 방법

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Also Published As

Publication number Publication date
US20200283711A1 (en) 2020-09-10
CN111164200A (zh) 2020-05-15
WO2019050101A9 (ko) 2019-04-11
EP3680321A4 (en) 2021-06-02
KR101847044B1 (ko) 2018-04-09
WO2019050101A2 (ko) 2019-03-14
EP3680321A2 (en) 2020-07-15

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