WO2019034748A1 - Liquid disinfectant for intensive animal houses - Google Patents
Liquid disinfectant for intensive animal houses Download PDFInfo
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- WO2019034748A1 WO2019034748A1 PCT/EP2018/072264 EP2018072264W WO2019034748A1 WO 2019034748 A1 WO2019034748 A1 WO 2019034748A1 EP 2018072264 W EP2018072264 W EP 2018072264W WO 2019034748 A1 WO2019034748 A1 WO 2019034748A1
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- Prior art keywords
- ammonium chloride
- liquid disinfectant
- alkyl dimethyl
- animal house
- alkyl
- Prior art date
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N33/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic nitrogen compounds
- A01N33/02—Amines; Quaternary ammonium compounds
- A01N33/12—Quaternary ammonium compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/18—Liquid substances or solutions comprising solids or dissolved gases
Definitions
- the present invention relates to a concentrated liquid sprayable disinfectant composition and the use of said composition for swine, poultry or broiler houses disinfection, for surface sterilization, and aerial disinfection.
- Viruses are widely responsible for immunosuppression in infected birds, which leads to poultry susceptible to opportunistic environmental pathogens present at sub-infective levels. Viruses are widely implicated in poultry epidemics of international economic importance. The direct costs of mortality as a result of endemic disease are immediately evident.
- preventative biosecurity is now a pre-requisite to poultry production, with poultry producers worldwide taking the necessary steps to institute strict biosecurity programs to exclude pathogenic organisms from farm environments.
- Terminal disinfection is the most effective part of an all-in, all-out production program, and is critical in ensuring a healthy start for a new batch of poultry chicks. This involves careful removal of poultry waste and litter, followed by intensive sanitization and disinfection to eliminate infectious organisms or reduce their populations to non-infective levels in poultry housing, and also from vehicles and equipment used.
- a good terminal hygiene program starting with pre-cleaning using a bactericidal detergent-sanitizer ensures removal of dirt and associated organisms.
- Routine farm practices such as hand sanitization, control of aerial respiratory transmission, management of manure and litter, water sanitization and pest control prevent the horizontal transmission of disease which can spread rapidly through the flock under intensive poultry production conditions.
- the concentrated animal house liquid disinfectant composition of the present invention overcome the foregoing problems by providing a stable composition in liquid form, a composition free of formaldehyde, safe in use for the end user in the concentrated and diluted form, being cost compatible with the pig and broiler industry, and providing a broad spectrum bactericidal, sporicidal and virucidal activity.
- the aim of the present invention is to provide a concentrated animal house liquid disinfectant composition comprising quaternary ammonium salts, orthopthalaldehyde, EDTA and isopropyl alcohol.
- a further aspect of the invention is to provide the use of this animal house liquid disinfectant composition for the disinfection of pig or poultry houses.
- a further object of the present invention is to provide a method of disinfecting pig or poultry houses comprising the step of spraying an animal house liquid disinfectant composition comprising quaternary ammonium salts, orthopthalaldehyde, EDTA and isopropyl alcohol.
- this invention relates, to a concentrated animal house liquid disinfectant composition
- a concentrated animal house liquid disinfectant composition comprising:
- w/v denotes a percentage concentration as a weight/volume percent or mass/volume percent. This percentage concentration indicates the amount of components in grams with respect to the amount of the composition in milliliters (i.e. with respect to 100ml of the total composition).
- Alkyl dimethyl benzyl ammonium chloride also known as benzalkonium chloride, is a type of cationic surfactant. It is an organic salt classified as a quaternary ammonium compound.
- Alkyl dimethyl benzyl ammonium chlorides are a mixture of alkyl benzyl dimethyl ammonium chlorides, in which the alkyl group has various even-numbered alkyl chain lengths. Any mixture of alkyls may be used, but preferably, the composition according to the present invention comprises about 5 % Cn alkyl, 60 % Ci 4 alkyl, 30 % Ci& alkyl, and about 5 % Cis alkyl dimethyl benzyl ammonium chloride.
- Alkyl dimethyl ethylbenzyl ammonium chloride is also a type of cationic surfactant. It is an organic salt classified as a quaternary ammonium compound.
- Alkyl dimethyl ethylbenzyl ammonium chlorides are a mixture of alkyl dimethyl benzyldimethyl ammonium chlorides, in which the alkyi group has various even-numbered alkyi chain lengths. Any mixture of alkyls may be used, but preferably, the composition according to the present invention comprises about 68 % Cn alkyi, and about 32 % Ci 4 alkyi dimethyl ethylbenzyl ammonium chloride.
- Orthophthalaldehyde or ortho-phthalaldehyde is the chemical compound with the formula C&H4(CHO)2.
- OPA ortho-phthalaldehyde
- the molecule is a dialdehyde, consisting of two formyl (CHO) groups attached to adjacent carbon centers on a benzene ring. OPA dissolves in water solution at pH ⁇ 11.5.
- EDTA tetra sodium salt (Ethylene Diamine Tetraacetic Acid) is used as a metal sequester improving the activity of the quaternary ammoniums.
- Isopropyl alcohol (l UPAC name propan-2-ol, CAS 67-63-0), also called dimethyl carbinoi or, isopropanoi, is a compound with the chemical formula CBHSO or C3H7OH or CH3CHOHCH3.
- the animal house liquid disinfectant composition according to the present invention comprises:
- the animal house liquid disinfectant composition according to the present invention comprises:
- compositions as described above can easily be prepared by the person skilled in the art following routine procedures. Basically, EDTA is first dissolved in water under stirring, followed by addition of isopropyl alcohol, then a mixture of alkyl dimethyl benzyl ammonium chloride and alkyl dimethyl ethylbenzyl ammonium chloride is added under continuous stirring and finally, the orthophtalaldehyde is added under stirring until complete dissolution. Volume is then adjusted with water.
- the composition is stable at up to 40°C for up to two years, and shows excellent sprayability characteristics ensuring a complete disinfection of surfaced as demonstrated by the inventors.
- the present invention also provides an animal house liquid disinfectant composition wherein the composition is prepared by diluting in tap water a concentrated animal house liquid disinfectant composition, as defined above, comprising: a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride
- the dilution is performed at a volume ratio of the concentrated animal house liquid disinfectant to water comprised between 1:20 and 1:200.
- the ratio of concentrated animal house liquid disinfectant to water is comprised between 1:30 and 1:120, preferably between 1:50 and 1:120, and even more preferably about 1:100.
- the term “about” will be understood by a person of ordinary skill in the art and will vary to some extent on the context in which it is used. If there are uses of the term which are not clear to persons of ordinary skill in the art given the context in which it is used, "about” will mean up to plus or minus 10 %, preferably 5 % of the particular term.
- the present invention provides the use of an animal house liquid disinfectant composition wherein the composition is prepared by diluting in water a concentrated animal house liquid disinfectant composition comprising:
- dilution is performed at a volume ratio of concentrated animal house liquid disinfectant to water comprised between 1:20 and 1:200 for the disinfection of pig or poultry houses.
- Said disinfectant solution is applied to the area to be disinfected in liquid form and is most efficiently carried out by spraying using any conventional sprayer standard in the industry.
- the present invention also provides a method of manufacturing a disinfectant solution comprising the steps of diluting a concentrated animal house liquid disinfectant composition comprising:
- liquid composition as described above leads to a complete killing and or inactivation of viruses, spores, bacteria, and/or fungi.
- the present invention provides a method of disinfecting animal houses comprising the step of spraying a diluted animal house liquid disinfectant composition comprising:
- dilution of said concentrate is performed at a ratio of concentrated animal house liquid disinfectant to water comprised between 1:20 and 1:200.
- Example 1 Stable concentrated animal house liquid disinfectant composition: alkyl dimethyl benzyl ammonium chloride 2 w/v % alkyl dimethyl ethylbenzyl ammonium chloride 2.5 w/v % orthopthalaldehyde 0.5 w/v %
- Example 2 Stable concentrated animal house liquid disinfectant composition: alkyl dimethyl benzyl ammonium chloride 2.5 w/v % alkyl dimethyl ethylbenzyl ammonium chloride 2.5 w/v % orthopthalaldehyde 0.5 w/v %
- Example 3 Stable concentrated animal house liquid disinfectant composition: alkyl dimethyl benzyl ammonium chloride 2.37 w/v % alkyl dimethyl ethylbenzyl ammonium chloride 2.37 w/v % orthopthalaldehyde 0.55 w/v %
- alkyl dimethyl benzyl ammonium chloride comprises about 5 % Ci2 alkyl, 60 % C i4 alkyl, 30 % Ci 6 alkyl, and about 5 % Ci 8 alkyl dimethyl benzyl ammonium chloride, while alkyl dimethyl ethylbenzyl ammonium chloride comprises about 68 % Ci2 alkyl, and about 32 % Ci 4 alkyl dimethyl ethylbenzyl ammonium chloride.
- composition is manufactured as follows: EDTA is first dissolved in water under stirring, followed by addition of isopropyl alcohol, then a mixture of alkyl dimethyl benzyl ammonium chloride and alkyl dimethyl ethylbenzyl ammonium chloride is added under continuous stirring and finally, the orthophtalaldehyde is added under stirring until complete dissolution. Volume is then adjusted with water.
- Example 4 Ready to use diluted animal house liquid disinfectant:
- the concentrated compositions according to Example 3 is diluted in water at a ratio of Concentrate/Water of 1/100 leading to the following compositions:
- Example 5 Stability studies of the concentrated animal house liquid disinfectant composition of Example 3
- the concentrated compositions according to Example 3 has been diluted in water at a ratio of Concentrate/Water of 1/100 to obtain solution A.
- solution A 0.1 ml of bacterial culture having a density of approximately 10 6 cfu/ml was added and kept at a temperature between 18 and 20 °C with intermittent shaking.
- time intervals Killing / Innactivation contact time of 5, 10 or 15 minutes
- a loopfull was added in a test tube containing sterile nutrient broth for bacteria l cultures, and sterile Sabouraud broth for fungal spp. Then all the nutrient broth tubes were incubated at 37°C for 48 hours, while Sabouraud broth tubes were incubated at room temperature in the dark for 72 hours to observe growth or inactivation.
- the results obtained are summarized below
- MRSA means the strain is a multi-resistant S. aureus.
Abstract
The present invention relates to a concentrated liquid sprayable disinfectant composition comprising an alkyl dimethyl benzyl ammonium chloride, an alkyl dimethyl ethylbenzyl ammonium chloride, ortho-pthalaldehyde, EDTA tetra sodium salt, isopropyl alcohol and water. Furthermore the invention also relates to the use of said composition for swine, poultry or broiler house disinfection, for surface sterilization, and aerial disinfection.
Description
LIQUID DISINFECTANT FOR INTENSIVE ANIMAL HOUSES
FIELD OF THE INVENTION The present invention relates to a concentrated liquid sprayable disinfectant composition and the use of said composition for swine, poultry or broiler houses disinfection, for surface sterilization, and aerial disinfection.
BACKGROUND OF THE INVENTION
Viruses are widely responsible for immunosuppression in infected birds, which leads to poultry susceptible to opportunistic environmental pathogens present at sub-infective levels. Viruses are widely implicated in poultry epidemics of international economic importance. The direct costs of mortality as a result of endemic disease are immediately evident.
Therefore, preventative biosecurity is now a pre-requisite to poultry production, with poultry producers worldwide taking the necessary steps to institute strict biosecurity programs to exclude pathogenic organisms from farm environments.
Terminal disinfection is the most effective part of an all-in, all-out production program, and is critical in ensuring a healthy start for a new batch of poultry chicks. This involves careful removal of poultry waste and litter, followed by intensive sanitization and disinfection to eliminate infectious organisms or reduce their populations to non-infective levels in poultry housing, and also from vehicles and equipment used. A good terminal hygiene program starting with pre-cleaning using a bactericidal detergent-sanitizer ensures removal of dirt and associated organisms.
While the direct costs of mortality as a result of endemic disease is immediately evident, the effect of subclinical infectious disease on poultry productivity is often underestimated. Minimizing environmental pathogen populations in poultry housing during the early growth period ensures that the immune system of birds is not
overwhelmed, hence provides better performance of vaccines, and reduces veterinary pharmaceutical usage and associated residues in poultry meat and eggs.
Routine farm practices such as hand sanitization, control of aerial respiratory transmission, management of manure and litter, water sanitization and pest control prevent the horizontal transmission of disease which can spread rapidly through the flock under intensive poultry production conditions.
One of the routine in the industry sanitization protocol consists in fumigating animal houses with formaldehyde vapor. However formalin (a clea r aq ueous solution of forma ldehyde a nd metha nol) is a highly toxic chemical that is already banned in some countries.
Therefore, there is still a need in the industry for a liquid disinfectant composition which is safe to the end user, environmental friendly, and provides a broad spectrum bactericidal, sporicidal, fungicidal, and virucidal activity.
SUMMARY OF THE INVENTION
Surprisingly, the inventors found that the concentrated animal house liquid disinfectant composition of the present invention overcome the foregoing problems by providing a stable composition in liquid form, a composition free of formaldehyde, safe in use for the end user in the concentrated and diluted form, being cost compatible with the pig and broiler industry, and providing a broad spectrum bactericidal, sporicidal and virucidal activity. In a first aspect, the aim of the present invention is to provide a concentrated animal house liquid disinfectant composition comprising quaternary ammonium salts, orthopthalaldehyde, EDTA and isopropyl alcohol.
A further aspect of the invention is to provide the use of this animal house liquid disinfectant composition for the disinfection of pig or poultry houses.
A further object of the present invention is to provide a method of disinfecting pig or poultry houses comprising the step of spraying an animal house liquid disinfectant composition comprising quaternary ammonium salts, orthopthalaldehyde, EDTA and isopropyl alcohol.
DETAILED DESCRIPTION OF THE INVENTION
In a first aspect, this invention relates, to a concentrated animal house liquid disinfectant composition comprising:
a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride
b. 2 to 3 w/v % alkyl dimethyl ethylbenzyl ammonium chloride
c. 0.4 to 0.6 w/v % orthopthalaldehyde
d. 0.025 to 0.075 w/v % EDTA tetra sodium salt
e. 8 to 12 w/v % isopropyl alcohol
f. Q.S water.
As used herein, the term "w/v " denotes a percentage concentration as a weight/volume percent or mass/volume percent. This percentage concentration indicates the amount of components in grams with respect to the amount of the composition in milliliters (i.e. with respect to 100ml of the total composition).
Alkyl dimethyl benzyl ammonium chloride also known as benzalkonium chloride, is a type of cationic surfactant. It is an organic salt classified as a quaternary ammonium compound. Alkyl dimethyl benzyl ammonium chlorides are a mixture of alkyl benzyl dimethyl ammonium chlorides, in which the alkyl group has various even-numbered alkyl chain lengths. Any mixture of alkyls may be used, but preferably, the composition according to the present invention comprises about 5 % Cn alkyl, 60 % Ci4 alkyl, 30 % Ci& alkyl, and about 5 % Cis alkyl dimethyl benzyl ammonium chloride.
Alkyl dimethyl ethylbenzyl ammonium chloride is also a type of cationic surfactant. It is an organic salt classified as a quaternary ammonium compound. Alkyl dimethyl ethylbenzyl ammonium chlorides are a mixture of alkyl dimethyl benzyldimethyl ammonium
chlorides, in which the alkyi group has various even-numbered alkyi chain lengths. Any mixture of alkyls may be used, but preferably, the composition according to the present invention comprises about 68 % Cn alkyi, and about 32 % Ci4 alkyi dimethyl ethylbenzyl ammonium chloride.
Orthophthalaldehyde or ortho-phthalaldehyde (OPA; CAS 643-79-8) is the chemical compound with the formula C&H4(CHO)2. Often abbreviated OPA, the molecule is a dialdehyde, consisting of two formyl (CHO) groups attached to adjacent carbon centers on a benzene ring. OPA dissolves in water solution at pH < 11.5.
EDTA tetra sodium salt (Ethylene Diamine Tetraacetic Acid) is used as a metal sequester improving the activity of the quaternary ammoniums.
Isopropyl alcohol (l UPAC name propan-2-ol, CAS 67-63-0), also called dimethyl carbinoi or, isopropanoi, is a compound with the chemical formula CBHSO or C3H7OH or CH3CHOHCH3.
I n a preferred embodiment, the animal house liquid disinfectant composition according to the present invention comprises:
a. 2.2 to 2.5 w/v % alkyi dimethyl benzyl ammonium Chloride
b. 2.2 to 2.5 w/v % alkyi dimethyl ethylbenzyl ammonium Chloride
c. 0.5 to 0.6 w/v % orthopthalaldehyde
d. 0.04 to 0.06 w/v % EDTA tetra sodium salt
e. 8 to 12 w/v % isopropyl alcohol
f. OS water.
Even more preferably, the animal house liquid disinfectant composition according to the present invention comprises:
a. 2.3 to 2.4 w/v % alkyi dimethyl benzyl ammonium Chloride
b. 2.3 to 2.4 w/v % alkyi dimethyl ethylbenzyl ammonium Chloride
c. 0.5 to 0.6 w/v % orthopthalaldehyde
d. 0.04 to 0.06 w/v % EDTA tetra sodium salt
e. 8 to 12 w/v % isopropyl alcohol
f. Q.S water.
The compositions as described above can easily be prepared by the person skilled in the art following routine procedures. Basically, EDTA is first dissolved in water under stirring, followed by addition of isopropyl alcohol, then a mixture of alkyl dimethyl benzyl ammonium chloride and alkyl dimethyl ethylbenzyl ammonium chloride is added under continuous stirring and finally, the orthophtalaldehyde is added under stirring until complete dissolution. Volume is then adjusted with water.
The composition is stable at up to 40°C for up to two years, and shows excellent sprayability characteristics ensuring a complete disinfection of surfaced as demonstrated by the inventors.
In another embodiment, the present invention also provides an animal house liquid disinfectant composition wherein the composition is prepared by diluting in tap water a concentrated animal house liquid disinfectant composition, as defined above, comprising: a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride
b. 2 to 3 w/v % alkyl dimethyl ethylbenzyl ammonium chloride
c. 0.4 to 0.6 w/v % orthopthalaldehyde
d. 0.025 to 0.075 w/v % EDTA tetra sodium salt
e. 8 to 12 w/v % isopropyl alcohol
f. Q.S water,
wherein the dilution is performed at a volume ratio of the concentrated animal house liquid disinfectant to water comprised between 1:20 and 1:200.
Preferably, the ratio of concentrated animal house liquid disinfectant to water is comprised between 1:30 and 1:120, preferably between 1:50 and 1:120, and even more preferably about 1:100.
As used herein, the term "about" will be understood by a person of ordinary skill in the art and will vary to some extent on the context in which it is used. If there are uses of the term which are not clear to persons of ordinary skill in the art given the context in which it is used, "about" will mean up to plus or minus 10 %, preferably 5 % of the particular term.
In yet another embodiment, the present invention provides the use of an animal house liquid disinfectant composition wherein the composition is prepared by diluting in water a concentrated animal house liquid disinfectant composition comprising:
a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride
b. 2 to 3 w/v % alkyl dimethyl ethylbenzyl ammonium chloride
c. 0.4 to 0.6 w/v % orthopthalaldehyde
d. 0.025 to 0.075 w/v % EDTA tetra sodium salt
e. 8 to 12 w/v % isopropyl alcohol
f. Q.S water,
wherein the dilution is performed at a volume ratio of concentrated animal house liquid disinfectant to water comprised between 1:20 and 1:200 for the disinfection of pig or poultry houses. Said disinfectant solution is applied to the area to be disinfected in liquid form and is most efficiently carried out by spraying using any conventional sprayer standard in the industry.
The present invention also provides a method of manufacturing a disinfectant solution comprising the steps of diluting a concentrated animal house liquid disinfectant composition comprising:
a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride
b. 2 to 3 w/v % alkyl dimethyl ethylbenzyl ammonium chloride
c. 0.4 to 0.6 w/v % orthopthalaldehyde
d. 0.025 to 0.075 w/v % EDTA tetra sodium salt
e. 8 to 12 w/v % isopropyl alcohol
f. Q.S water,
In water in a volume ratio concentrated animal house liquid disinfectant:water comprised between 1:20 and 1:200.
The use of the liquid composition as described above leads to a complete killing and or inactivation of viruses, spores, bacteria, and/or fungi.
In a last embodiment, the present invention provides a method of disinfecting animal houses comprising the step of spraying a diluted animal house liquid disinfectant composition comprising:
a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride
b. 2 to 3 w/v % alkyl dimethyl ethylbenzyl ammonium chloride
c. 0.4 to 0.6 w/v % orthopthalaldehyde
d. 0.025 to 0.075 w/v % EDTA tetra sodium salt
e. 8 to 12 w/v % isopropyl alcohol
f. Q.S water,
wherein the dilution of said concentrate is performed at a ratio of concentrated animal house liquid disinfectant to water comprised between 1:20 and 1:200.
All embodiments described above for the stable concentrated animal house liquid disinfectant composition also apply to the use of said composition, and to the method of disinfection as described above.
EXAMPLES
Example 1: Stable concentrated animal house liquid disinfectant composition: alkyl dimethyl benzyl ammonium chloride 2 w/v % alkyl dimethyl ethylbenzyl ammonium chloride 2.5 w/v % orthopthalaldehyde 0.5 w/v %
EDTA tetra sodium salt 0.05 w/v % isopropyl alcohol 10 w/v % water qsp
Example 2: Stable concentrated animal house liquid disinfectant composition: alkyl dimethyl benzyl ammonium chloride 2.5 w/v % alkyl dimethyl ethylbenzyl ammonium chloride 2.5 w/v % orthopthalaldehyde 0.5 w/v %
EDTA tetra sodium salt 0.05 w/v % isopropyl alcohol 12 w/v % water qsp
Example 3: Stable concentrated animal house liquid disinfectant composition: alkyl dimethyl benzyl ammonium chloride 2.37 w/v % alkyl dimethyl ethylbenzyl ammonium chloride 2.37 w/v % orthopthalaldehyde 0.55 w/v %
EDTA tetra sodium salt 0.05 w/v % isopropyl alcohol 10 w/v % water qsp
In the above examples 1 to 3, alkyl dimethyl benzyl ammonium chloride comprises about 5 % Ci2 alkyl, 60 % Ci4 alkyl, 30 % Ci6 alkyl, and about 5 % Ci8 alkyl dimethyl benzyl ammonium chloride, while alkyl dimethyl ethylbenzyl ammonium chloride comprises about 68 % Ci2 alkyl, and about 32 % Ci4 alkyl dimethyl ethylbenzyl ammonium chloride. Furthermore, the composition is manufactured as follows: EDTA is first dissolved in water under stirring, followed by addition of isopropyl alcohol, then a mixture of alkyl dimethyl benzyl ammonium chloride and alkyl dimethyl ethylbenzyl ammonium chloride is added under continuous stirring and finally, the orthophtalaldehyde is added under stirring until complete dissolution. Volume is then adjusted with water.
Example 4: Ready to use diluted animal house liquid disinfectant:
To prepare the ready to use composition, the concentrated compositions according to Example 3 is diluted in water at a ratio of Concentrate/Water of 1/100 leading to the following compositions:
Ready to Use Ready to Use Ready to Use composition 1 composition 2 composition 3 alkyl dimethyl
benzyl ammonium 0.02 0.025 0.0237 chloride (w/v %)
alkyl dimethyl
ethylbenzyl
0.025 0.025 0.0237 ammonium chloride
(w/v °/o)
Orthopthalaldehyde
0.005 0.005 0.0055
(w/v °/o)
EDTA tetra sodium
0.0005 0.0005 0.0005 salt (w/v %)
isopropyl alcohol
0.10 0.12 0.10
(w/v °/o)
water qsp qsp qsp
Example 5: Stability studies of the concentrated animal house liquid disinfectant composition of Example 3
Example 6: Antimicrobial efficacy assays
The concentrated compositions according to Example 3 has been diluted in water at a ratio of Concentrate/Water of 1/100 to obtain solution A. To 5 ml aliquot of Solution A, 0.1 ml of bacterial culture having a density of approximately 106 cfu/ml was added and kept at a temperature between 18 and 20 °C with intermittent shaking. After different time intervals (Killing / Innactivation contact time of 5, 10 or 15 minutes), a loopfull was added in a test tube containing sterile nutrient broth for bacteria l cultures, and sterile Sabouraud broth for fungal spp. Then all the nutrient broth tubes were incubated at 37°C for 48 hours, while Sabouraud broth tubes were incubated at room temperature in the dark for 72 hours to observe growth or inactivation.
The results obtained are summarized below
N means no viable organisms have been observed; V means organisms were observed. MRSA means the strain is a multi-resistant S. aureus.
This data confirms the disinfecting efficacy of the composition according to the present invention after a contact time of 5 minutes or less for bacteria as well as for fungi.
Claims
1. A concentrated animal house liquid disinfectant composition comprising:
a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride,
b. 2 to 3 w/v % alkyl dimethyl ethylbenzyl ammonium chloride,
c. 0.4 to 0.6 w/v % orthopthalaldehyde,
d. 0.025 to 0.075 w/v % EDTA tetra sodium salt,
e. 8 to 12 w/v % isopropyl alcohol, and
f. Q.S water.
2. The concentrated animal house liquid disinfectant composition according to claim 1, wherein the composition comprises:
a. 2.2 to 2.5 w/v % alkyl dimethyl benzyl ammonium chloride,
b. 2.2 to 2.5 w/v % alkyl dimethyl ethylbenzyl ammonium chloride,
c. 0.5 to 0.6 w/v % orthopthalaldehyde,
d. 0.04 to 0.06 w/v % EDTA tetra sodium salt,
e. 8 to 12 w/v % isopropyl alcohol, and
f. Q.S water.
3. The concentrated anima l house liquid disinfectant composition according to claims 1 or 2, wherein the alkyl dimethyl benzyl ammonium chloride is mixture of about 5 % Ci2 alkyl, 60 % Ci4 alkyl, 30 % Ci6 alkyl, and about 5 % Ci8 alkyl.
4. The concentrated animal house liquid disinfectant composition according to any of the claims 1 to 3, wherein the alkyl dimethyl ethylbenzyl ammonium chloride is mixture of about 68 % C12 alkyl, and about 32 % Ci4 alkyl.
5. The concentrated animal house liquid disinfectant composition according to any of the claims 1 to 4, wherein the composition comprises:
a. 2.3 to 2.4 w/v % alkyl dimethyl benzyl ammonium chloride,
b. 2.3 to 2.4 w/v % alkyl dimethyl ethylbenzyl ammonium chloride,
c. 0.5 to 0.6 w/v % orthopthalaldehyde,
d. 0.04 to 0.06 w/v % EDTA tetra sodium salt,
e. 8 to 12 w/v % isopropyl alcohol, and
f. Q.S water.
6. An animal house liquid disinfectant composition wherein the composition is prepared by diluting a concentrated animal house liquid disinfectant composition according to any of the claims 1 to 5, in water at a ratio of concentrated animal house liquid disinfectant to water comprised between 1:20 and 1:200.
7. The animal house liquid disinfectant composition according to claim 6, wherein the ratio of concentrated animal house liquid disinfectant to water is comprised between 1: 50 and 1:120.
8. Use of an animal house liquid disinfectant according to any of the claims 6 or 7 for the disinfection of pig or poultry houses.
9. The use according to claim 8, wherein the disinfection is carried out by spraying.
10. The use according to claims 8 or 9, wherein the disinfection is characterized by a complete killing and or inactivation of viruses, spores, bacteria, and/or fungi.
11. A method of disinfecting animal houses comprising the step of spraying a diluted animal house liquid disinfectant composition comprising:
a. 2 to 3 w/v % alkyl dimethyl benzyl ammonium chloride,
b. 2 to 3 w/v % alkyl dimethyl ethylbenzyl ammonium chloride,
c. 0.4 to 0.6 w/v % orthopthalaldehyde,
d. 0.025 to 0.075 w/v % EDTA tetra sodium salt,
e. 8 to 12 w/v % isopropyl alcohol, and
f. Q.S water,
wherein the dilution is performed at a ratio of concentrated animal house liquid disinfectant to water comprised between 1: 20 and 1:200.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN201711028993 | 2017-08-16 | ||
| IN201711028993 | 2017-08-16 | ||
| EP17194873 | 2017-10-05 | ||
| EP17194873.0 | 2017-10-05 |
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|---|---|
| WO2019034748A1 true WO2019034748A1 (en) | 2019-02-21 |
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| US20110059027A1 (en) * | 2009-09-09 | 2011-03-10 | Howard Martin | Biocidal aldehyde composition |
| CN105494339A (en) * | 2016-02-02 | 2016-04-20 | 成都科宏达科技有限公司 | Disinfectant for disinfection of livestock and poultry farms, as well as application thereof |
| CN105532660A (en) * | 2016-02-02 | 2016-05-04 | 成都科宏达科技有限公司 | Compound disinfectant and application thereof |
-
2018
- 2018-08-16 WO PCT/EP2018/072264 patent/WO2019034748A1/en active Application Filing
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| US5124359A (en) * | 1987-12-22 | 1992-06-23 | Cetylite Industries, Inc. | Sterilant composition |
| FR2743982A1 (en) * | 1996-01-30 | 1997-08-01 | Salkin Andre | Combination of aromatic aldehyde and quat. ammonium derivs. as antimicrobial agent |
| WO2009100879A2 (en) * | 2008-02-12 | 2009-08-20 | Lonza Inc. | Broad spectrum disinfecting and sterilizing composition |
| US20110059027A1 (en) * | 2009-09-09 | 2011-03-10 | Howard Martin | Biocidal aldehyde composition |
| CN105494339A (en) * | 2016-02-02 | 2016-04-20 | 成都科宏达科技有限公司 | Disinfectant for disinfection of livestock and poultry farms, as well as application thereof |
| CN105532660A (en) * | 2016-02-02 | 2016-05-04 | 成都科宏达科技有限公司 | Compound disinfectant and application thereof |
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