WO2019031595A1 - Procédé de criblage de cellules rénales progénitrices - Google Patents

Procédé de criblage de cellules rénales progénitrices Download PDF

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Publication number
WO2019031595A1
WO2019031595A1 PCT/JP2018/029999 JP2018029999W WO2019031595A1 WO 2019031595 A1 WO2019031595 A1 WO 2019031595A1 JP 2018029999 W JP2018029999 W JP 2018029999W WO 2019031595 A1 WO2019031595 A1 WO 2019031595A1
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WIPO (PCT)
Prior art keywords
mir
cells
mirna
cell
mrna
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PCT/JP2018/029999
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English (en)
Japanese (ja)
Inventor
高橋　淳
飛鳥 森実
匡 春原
博英 齊藤
カラム パー
萌 余越
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国立大学法人京都大学
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Application filed by 国立大学法人京都大学 filed Critical 国立大学法人京都大学
Priority to JP2019535722A priority Critical patent/JP7236738B2/ja
Publication of WO2019031595A1 publication Critical patent/WO2019031595A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids

Abstract

La présente invention concerne un procédé de sélection de cellules progénitrices neurales, comprenant : (1) une étape d'introduction, dans les cellules, d'un ARNm dans lequel une séquence codant pour une protéine marqueur est fonctionnellement liée à une séquence reconnue par un miARN exprimé dans les cellules progénitrices neurales, ou un ADN qui code pour ledit ARNm; et (2) une étape de criblage de cellules dans lesquelles l'expression de ladite protéine marqueur est supprimée.
PCT/JP2018/029999 2017-08-10 2018-08-09 Procédé de criblage de cellules rénales progénitrices WO2019031595A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2019535722A JP7236738B2 (ja) 2017-08-10 2018-08-09 神経前駆細胞の選別方法

Applications Claiming Priority (2)

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JP2017156213 2017-08-10
JP2017-156213 2017-08-10

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WO2019031595A1 true WO2019031595A1 (fr) 2019-02-14

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PCT/JP2018/029999 WO2019031595A1 (fr) 2017-08-10 2018-08-09 Procédé de criblage de cellules rénales progénitrices

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JP (1) JP7236738B2 (fr)
WO (1) WO2019031595A1 (fr)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111022066A (zh) * 2019-11-11 2020-04-17 北京住总集团有限责任公司 一种基于bim与gis的盾构机风险源穿越三维模拟及监控系统
CN112458165A (zh) * 2020-12-27 2021-03-09 中山大学孙逸仙纪念医院 一种阿尔茨海默病的预测标志物、检测试剂盒及应用
CN114540480A (zh) * 2022-01-10 2022-05-27 南方医科大学 miR-501-3p作为精神分裂症诊断标志物的应用
EP4073235A4 (fr) * 2019-12-09 2024-02-14 Wisconsin Alumni Res Found Multiplication in vitro de progéniteurs neuronaux de sous-type dopaminergiques dérivés de cellules souches pluripotentes

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013501502A (ja) * 2009-08-12 2013-01-17 国立大学法人京都大学 多能性幹細胞から神経前駆細胞への分化誘導法
WO2015141827A1 (fr) * 2014-03-20 2015-09-24 国立大学法人京都大学 Procédé de tri de cardiomyocytes
WO2016114354A1 (fr) * 2015-01-14 2016-07-21 国立大学法人京都大学 Procédé de sélection de cellule progénitrice des muscles squelettiques
WO2016171235A1 (fr) * 2015-04-22 2016-10-27 国立大学法人京都大学 Méthode de tri de cellules tissulaires

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2440660A4 (fr) * 2009-06-10 2013-08-28 Brainstem Biotec Ltd Procédés, systèmes et compositions pour la différentiation neuronale de cellules stromales pluripotentes

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2013501502A (ja) * 2009-08-12 2013-01-17 国立大学法人京都大学 多能性幹細胞から神経前駆細胞への分化誘導法
WO2015141827A1 (fr) * 2014-03-20 2015-09-24 国立大学法人京都大学 Procédé de tri de cardiomyocytes
WO2016114354A1 (fr) * 2015-01-14 2016-07-21 国立大学法人京都大学 Procédé de sélection de cellule progénitrice des muscles squelettiques
WO2016171235A1 (fr) * 2015-04-22 2016-10-27 国立大学法人京都大学 Méthode de tri de cellules tissulaires

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
MIKI, K. ET AL.: "Efficient detection and purification of cell populations using synthetic microRNA switches", CELL STEM CELL, vol. 16, no. 6, June 2015 (2015-06-01), pages 699 - 711, XP055225629 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111022066A (zh) * 2019-11-11 2020-04-17 北京住总集团有限责任公司 一种基于bim与gis的盾构机风险源穿越三维模拟及监控系统
CN111022066B (zh) * 2019-11-11 2021-10-01 北京住总集团有限责任公司 一种基于bim与gis的盾构机风险源穿越三维模拟及监控系统
EP4073235A4 (fr) * 2019-12-09 2024-02-14 Wisconsin Alumni Res Found Multiplication in vitro de progéniteurs neuronaux de sous-type dopaminergiques dérivés de cellules souches pluripotentes
CN112458165A (zh) * 2020-12-27 2021-03-09 中山大学孙逸仙纪念医院 一种阿尔茨海默病的预测标志物、检测试剂盒及应用
CN114540480A (zh) * 2022-01-10 2022-05-27 南方医科大学 miR-501-3p作为精神分裂症诊断标志物的应用

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JPWO2019031595A1 (ja) 2020-07-09
JP7236738B2 (ja) 2023-03-10

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