WO2019030956A1 - Black raspberry extract, skin lightening agent and food or beverage product including black raspberry extract, production method for black raspberry extract, and skin lightening method using black raspberry extract - Google Patents

Black raspberry extract, skin lightening agent and food or beverage product including black raspberry extract, production method for black raspberry extract, and skin lightening method using black raspberry extract Download PDF

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WO2019030956A1
WO2019030956A1 PCT/JP2018/006356 JP2018006356W WO2019030956A1 WO 2019030956 A1 WO2019030956 A1 WO 2019030956A1 JP 2018006356 W JP2018006356 W JP 2018006356W WO 2019030956 A1 WO2019030956 A1 WO 2019030956A1
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black raspberry
extract
raspberry extract
black
food
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PCT/JP2018/006356
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French (fr)
Japanese (ja)
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晴隆 坂野
侑子 楠見
安則 金保
敦士 高田
克昭 團
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有限会社オービット
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Priority to CN202310470790.9A priority Critical patent/CN116370382A/en
Priority to CN201880004850.5A priority patent/CN110139569A/en
Publication of WO2019030956A1 publication Critical patent/WO2019030956A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
    • A61K2800/52—Stabilizers
    • A61K2800/522—Antioxidants; Radical scavengers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/74—Biological properties of particular ingredients
    • A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
    • A61K2800/782—Enzyme inhibitors; Enzyme antagonists
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
    • A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin

Definitions

  • the present invention relates to a black raspberry extract, a skin lightening agent containing black raspberry extract and food and drink, a method for producing a black raspberry extract and a whitening method using the black raspberry extract.
  • oxygen is essential for most organisms, a few percent of the oxygen taken into the body is reactive oxygen and is used to protect against bacterial infections and the like. However, excess active oxygen needs to be eliminated by in vivo antioxidant enzymes and foreign antioxidants in food in order to maintain homeostasis.
  • Non-Patent Document 1 when excessive active oxygen is produced by ultraviolet light, smoking, exhaust gas, air pollution, stress or the like, the biological tissue is oxidatively damaged (see, for example, Non-Patent Document 1). As a result, lifestyle-related diseases, age-related diseases, cancer, dementia, etc. are caused, and 90% or more of the diseases are said to have a causal relationship with the overproduced active oxygen (eg, non- Patent Document 2).
  • melanin pigments responsible for pigmentation in the skin is promoted by various exogenous and endogenous stimuli.
  • active oxygen is generated on the surface of the skin, and the stimulation activates melanocytes to enhance tyrosinase expression.
  • the tyrosinase thus expressed metabolizes tyrosine, and through several steps the melanin pigment is produced. It has been reported that the production of melanin pigment is suppressed by capturing this active oxygen with an antioxidant (see, for example, Non-Patent Document 5).
  • tyrosinase activity in melanocytes skin lightening effects can be expected.
  • the object of the present invention is to use a berry-based black raspberry extract having an action of suppressing the production of melanin pigment, a skin lightening agent and food and drink containing black raspberry extract, a method of producing black raspberry extract and black raspberry extract To provide a whitening method.
  • the present inventors focused on berry-based food as food having antioxidant power, and examined the correlation between berry-based food and the action of suppressing the production of the above-mentioned melanin pigment, and as a result, extracts of black raspberry Discovered that the above objects have been achieved, and completed the present invention.
  • a black raspberry extract having the effect of inhibiting the activity of tyrosinase enzyme in melanocytes (2) A whitening agent comprising the black raspberry extract according to (1), (3) Food and drink containing the black raspberry extract according to (1), (4) A method for producing a black raspberry extract according to (1), comprising the steps of: freezing the black raspberry fruit; and thawing the black raspberry fruit.
  • a black raspberry including a stirring step of adding and stirring a solvent to the black raspberry fruit thawed in the thawing step, and an extraction step of leaving the fruit and solvent of the black raspberry stirred in the stirring step and extracting
  • a method of producing an extract (5) A whitening method using the black raspberry extract according to (1) is provided.
  • the present invention it is possible to provide a black raspberry extract, a skin lightening agent and food and drink containing the black raspberry extract, a method of producing the black raspberry extract and a whitening method using the black raspberry extract.
  • the black raspberry extract of the present invention has an activity inhibitory effect on tyrosinase enzyme in melanocytes.
  • Black raspberry which becomes a raw material of a black raspberry extract belongs to Rosaceae raspberry genus.
  • an extract mainly from black raspberry fruits is preferably used as the black raspberry extract of the present invention.
  • the method for extracting black raspberry extract from black raspberry fruits is not particularly limited, but solvent extraction, supercritical extraction and the like can be used.
  • Water an organic solvent, and these mixed solvents can be used.
  • organic solvent alcohols such as methanol, ethanol, n-propanol, isopropanol and n-butanol, glycols such as ethylene glycol and propylene glycol, ethyl acetate, acetone and the like can be used.
  • One of these organic solvents may be used alone, or two or more of these organic solvents may be used in combination.
  • an aqueous solvent such as water and an organic solvent may be mixed and used.
  • ethanol an aqueous ethanol solution in which ethanol and water are mixed, is preferable, and the concentration of ethanol in the aqueous ethanol solution is preferably 1 to 80 v / v% (EtOH), more preferably 10 to 60 v / v% (EtOH And more preferably 20 to 40 v / v% (EtOH).
  • EtOH v / v%
  • EtOH v / v%
  • stirring extraction immersion extraction, shaking extraction, reflux extraction, ultrasonic extraction etc.
  • stirring extraction is preferable.
  • the method of stirring and extracting is not particularly limited, but stirring using a stirrer, stirring using a blender, and the like can be performed.
  • stirring and extraction When stirring and extraction is carried out, it is preferably several minutes to 60 minutes, more preferably 30 minutes to 60 minutes while stirring at a stirring speed of preferably 500 to 20000 rpm, more preferably 1000 to 10000 rpm, still more preferably 6000 to 10000 rpm. Extract for 60 minutes.
  • a stirring speed preferably 500 to 20000 rpm, more preferably 1000 to 10000 rpm, still more preferably 6000 to 10000 rpm.
  • Extract for 60 minutes.
  • solid-liquid separation can be performed by leaving still and performing extraction.
  • the temperature for solvent extraction may be room temperature or heating conditions.
  • the pressure in the case of solvent extraction may be normal pressure or may be under pressure.
  • the extract obtained by the solvent extraction may be subjected to a treatment such as filtration as necessary.
  • the extract obtained by solvent extraction may be dried to form a powder.
  • a publicly known method can be used as a drying method, methods such as spray drying, vacuum drying, lyophilization and the like can be used.
  • a black raspberry extract you may use the commercial item made into liquid or powder form, or an existing product.
  • the black raspberry extract of the present invention can be blended into foods, beverages and the like.
  • the food include breads, noodles, confectionery, processed meat products, processed fish products, frozen foods, jellies, ice creams, dairy products, various seasonings and the like.
  • it can also be formulated into specified health foods, quasi-drugs, health foods, and supplements.
  • the beverage include soft drinks, milk drinks, liquors, tea, black tea drinks, coffee, fruit juice drinks, carbonated drinks, mineral waters, fruits and vegetable drinks and the like.
  • the food or beverage mixed with the black raspberry extract of the present invention may be in the same form as an oral preparation such as tablets, capsules and syrups.
  • a sweetener such as additives, gelling agents, antioxidants, color formers, bleaches, emulsifiers, expanders, acidulants, brighteners, perfumes, solvents, oils may be added.
  • additives such as additives, gelling agents, antioxidants, color formers, bleaches, emulsifiers, expanders, acidulants, brighteners, perfumes, solvents, oils may be added.
  • One of these additives may be used alone, or two or more thereof may be used in combination.
  • the proportion of the black raspberry extract incorporated in the above food or beverage can be appropriately adjusted according to the purpose of use, but the proportion of the black raspberry extract incorporated in the above food or beverage is preferably 0. .0001 to 80% by weight, more preferably 0.003 to 50% by weight, and still more preferably 0.005 to 30% by weight.
  • the black raspberry extract of the present invention is useful for obtaining a skin whitening effect.
  • BR black raspberry
  • BB blueberries
  • An extract of raspberry (Raspberry raspberry) (hereinafter sometimes referred to as "RB”)) and a powder preparation, Blabina (registered trademark), which contains an extract of black raspberry, were used.
  • Extracts of black raspberry, blueberry and raspberry were obtained by the following procedure. First, black raspberry, blueberry and raspberry fruits were each stored frozen. Next, each fruit was thawed and cut. Weigh each black raspberry, blueberry and raspberry (about 100 g), add 5 volumes of 30 v / v% (EtOH) aqueous ethanol (approximately 500 mL) and stir extract at 8000 rpm for 30 minutes using a blender went. Furthermore, stationary extraction was performed by leaving still at room temperature for 24 hours, and filtration through 150 mesh was performed to obtain ethanol extracts of the above black raspberry, blueberry and raspberry, respectively. Since measurement of ORAC described later is performed in an ethanol-dissolved state, measurement was performed using an ethanol extract. In addition, about Blabina, it dissolved so that it might become 9 mg / mL in 30 v / v% (EtOH).
  • the ethanol component was removed by evaporation using an evaporator from the ethanol extract of black raspberry, blueberry and raspberry obtained in the above preparation of the extract, and the ethanol solution of Blabina, respectively, to obtain an extract. Furthermore, the ethanol extract of black raspberry, blueberry and raspberry contained 9 mg / mL of extract (extract).
  • ORAC value The ORAC values of three types of berry samples (black raspberry, blueberry and ethanol extract of raspberry) obtained above were measured. The results are shown in Table 1. The ORAC value was not measured for Blatina.
  • the ORAC values of the three berry samples were all higher than those of the general antioxidant food (see Non-Patent Document 4 above for the ORAC value of the general antioxidant food).
  • blueberry showed the highest value, but this is a result of measurement using an ethanol extract, so it can not be mentioned about water-soluble ingredients.
  • melanocytes were used.
  • melanocytes use Normal Human Epidermal Melanocytes, juvenile foreskin (C-12400, purchased from PromoCell) and a dedicated medium (Melanocyte Growth Medium Supplement Pack; C-39410, purchased from PromoCell) at 37 ° C, 5 and cultured in% CO 2 conditions.
  • C-12400 juvenile foreskin
  • C-39410 dedicated medium
  • the cells were recovered with a 0.25% Trypsin / EDTA solution, and the number of cells was stained with trypan blue and then counted by Countess (Invitrogen). Thereafter, the cells were seeded in a 24-well plate or a 96-well plate, precultured for 24 hours, and then used for experiments.
  • the three berry-based (black raspberry, blueberry and raspberry) extracts obtained in the preparation of the above extract, and the ethanol-removed extract of Blabina are each dissolved in ultrapure water to about 11.5 mg / mL. , Got the sample. Next, each sample was serially diluted (100000-fold dilution to 10-fold dilution) and added to melanocytes, respectively.
  • the evaluation of the enzyme activity was indicated by using the absorbance (475 nm) of DOPA chromium synthesized by tyrosinase.
  • vitamin-C 20 mg / mL was used as positive control (For example, the said nonpatent literature 5 and Matsuki M, Watanabe T, Ogasawara A, Mikami T, Matsumoto T. Inhibitory mechanism of melanin synthesis by glutathione. [Article in Japanese Yakugaku Zasshi. 2008 Aug; 128 (8): 1203-7 12.).

Abstract

This black raspberry extract has an inhibitory effect on tyrosinase enzyme activity in melanocytes.

Description

ブラックラズベリー抽出物、ブラックラズベリー抽出物を含む美白剤および飲食物、ブラックラズベリー抽出物の製造方法およびブラックラズベリー抽出物を用いた美白方法Black raspberry extract, whitening agent and food containing black raspberry extract, method of preparing black raspberry extract and whitening method using black raspberry extract
　本発明は、ブラックラズベリー抽出物、ブラックラズベリー抽出物を含む美白剤および飲食物、ブラックラズベリー抽出物の製造方法およびブラックラズベリー抽出物を用いた美白方法に関するものである。 The present invention relates to a black raspberry extract, a skin lightening agent containing black raspberry extract and food and drink, a method for producing a black raspberry extract and a whitening method using the black raspberry extract.
　ほとんどの生物にとって酸素は不可欠であるが、体内に取り込まれた酸素の数％は活性酸素となり、細菌感染などの防御に使用される。しかし、余剰の活性酸素は、恒常性を維持するために生体内の抗酸化酵素や食品中の外来の抗酸化物質により消去されることが必要である。 Although oxygen is essential for most organisms, a few percent of the oxygen taken into the body is reactive oxygen and is used to protect against bacterial infections and the like. However, excess active oxygen needs to be eliminated by in vivo antioxidant enzymes and foreign antioxidants in food in order to maintain homeostasis.
　また紫外線、喫煙、排気ガス、大気汚染、ストレスなどによって過剰の活性酸素が産生されると生体組織へ酸化的障害を与える（例えば、非特許文献１参照）。その結果、生活習慣病、加齢性疾患をはじめ、がん、痴呆症などを引き起こし、９０％以上の疾病は過剰に産生される活性酸素と因果関係があると言われている（例えば、非特許文献２参照）。 In addition, when excessive active oxygen is produced by ultraviolet light, smoking, exhaust gas, air pollution, stress or the like, the biological tissue is oxidatively damaged (see, for example, Non-Patent Document 1). As a result, lifestyle-related diseases, age-related diseases, cancer, dementia, etc. are caused, and 90% or more of the diseases are said to have a causal relationship with the overproduced active oxygen (eg, non- Patent Document 2).
　そこで、体内のSuperoxide dismutase (SOD)の活性化とともに食品中の抗酸化物質が注目され、アメリカ農務省（USDA）で開発された抗酸化力の測定方法（ＯＲＡＣ；Oxygen Radical Absorbance Capacity）が確立され、それが普及している（例えば、非特許文献３参照）。また、ＯＲＡＣ値の高い食品、即ち抗酸化力の高い食品ベスト１００の中に１２品目のベリー系食品が存在している（例えば、非特許文献４参照）。 Therefore, with the activation of Superoxide dismutase (SOD) in the body, antioxidants in foods are attracting attention, and the method of measuring antioxidant capacity (ORAC; Oxygen Radical Absorbance Capacity) developed by the US Department of Agriculture (USDA) is established. , It is popular (see, for example, non-patent document 3). In addition, 12 items of berry-based food exist in the food with the high ORAC value, ie, the food product 100 with high antioxidant power (see, for example, Non-Patent Document 4).
　皮膚における色素沈着の原因となるメラニン色素の合成は種々の外来性及び内因性の刺激によって促進される。たとえば、太陽光の中の紫外線、ストレス、大気汚染などに曝されると皮膚の表面で活性酸素が発生し、その刺激によってメラノサイトが活性化され、チロシナーゼの発現が亢進する。こうして発現したチロシナーゼはチロシンを代謝し、いくつかの段階を経てメラニン色素が産生される。この活性酸素を抗酸化物質により捕捉することでメラニン色素の産生が抑制されることが報告されている（例えば、非特許文献５参照）。また、メラノサイトにおいてチロシナーゼの活性を阻害することができれば、肌の美白効果についても期待することができる。 The synthesis of melanin pigments responsible for pigmentation in the skin is promoted by various exogenous and endogenous stimuli. For example, when exposed to ultraviolet light in sunlight, stress, air pollution, and the like, active oxygen is generated on the surface of the skin, and the stimulation activates melanocytes to enhance tyrosinase expression. The tyrosinase thus expressed metabolizes tyrosine, and through several steps the melanin pigment is produced. It has been reported that the production of melanin pigment is suppressed by capturing this active oxygen with an antioxidant (see, for example, Non-Patent Document 5). In addition, if it is possible to inhibit tyrosinase activity in melanocytes, skin lightening effects can be expected.
　本発明の目的は、メラニン色素の産生を抑制する作用を有するベリー系のブラックラズベリー抽出物、ブラックラズベリー抽出物を含む美白剤および飲食物、ブラックラズベリー抽出物の製造方法およびブラックラズベリー抽出物を用いた美白方法を提供することである。 The object of the present invention is to use a berry-based black raspberry extract having an action of suppressing the production of melanin pigment, a skin lightening agent and food and drink containing black raspberry extract, a method of producing black raspberry extract and black raspberry extract To provide a whitening method.
　本発明者らは、抗酸化力を有する食品として、ベリー系食品に着目し、ベリー系食品と上記メラニン色素の産生を抑制する作用との相関性について検討し、その結果、ブラックラズベリーの抽出物が上記目的を達成することを発見し、本発明を完成するに至った。 The present inventors focused on berry-based food as food having antioxidant power, and examined the correlation between berry-based food and the action of suppressing the production of the above-mentioned melanin pigment, and as a result, extracts of black raspberry Discovered that the above objects have been achieved, and completed the present invention.
　即ち、本発明によれば、
（１）　メラノサイトにおけるチロシナーゼ酵素の活性阻害効果を有するブラックラズベリー抽出物、
（２）　（１）に記載のブラックラズベリー抽出物を含む美白剤、
（３）　（１）に記載のブラックラズベリー抽出物を含む飲食物、
（４）　（１）に記載のブラックラズベリー抽出物の製造方法であって、ブラックラズベリーの果実を冷凍する冷凍工程と、前記ブラックラズベリーの果実を解凍する解凍工程と、
　前記解凍工程にて解凍した前記ブラックラズベリーの果実に溶媒を加えて撹拌する撹拌工程と、前記撹拌工程により撹拌した前記ブラックラズベリーの果実および溶媒を静置し抽出を行う抽出工程とを含むブラックラズベリー抽出物の製造方法、
（５）　（１）に記載のブラックラズベリー抽出物を用いた美白方法
が提供される。 That is, according to the present invention,
(1) A black raspberry extract having the effect of inhibiting the activity of tyrosinase enzyme in melanocytes,
(2) A whitening agent comprising the black raspberry extract according to (1),
(3) Food and drink containing the black raspberry extract according to (1),
(4) A method for producing a black raspberry extract according to (1), comprising the steps of: freezing the black raspberry fruit; and thawing the black raspberry fruit.
A black raspberry including a stirring step of adding and stirring a solvent to the black raspberry fruit thawed in the thawing step, and an extraction step of leaving the fruit and solvent of the black raspberry stirred in the stirring step and extracting A method of producing an extract,
(5) A whitening method using the black raspberry extract according to (1) is provided.
　本発明によれば、ブラックラズベリー抽出物、ブラックラズベリー抽出物を含む美白剤および飲食物、ブラックラズベリー抽出物の製造方法およびブラックラズベリー抽出物を用いた美白方法を提供することができる。 According to the present invention, it is possible to provide a black raspberry extract, a skin lightening agent and food and drink containing the black raspberry extract, a method of producing the black raspberry extract and a whitening method using the black raspberry extract.
メラノサイトにおけるチロシナーゼ酵素の活性阻害効果の検討結果を示すグラフである。It is a graph which shows the examination result of the activity inhibitory effect of the tyrosinase enzyme in melanocytes.
　以下、本発明のブラックラズベリー抽出物について説明する。本発明のブラックラズベリー抽出物は、メラノサイトにおけるチロシナーゼ酵素の活性阻害効果を有する。 Hereinafter, the black raspberry extract of the present invention will be described. The black raspberry extract of the present invention has an activity inhibitory effect on tyrosinase enzyme in melanocytes.
　ブラックラズベリー抽出物の原料となるブラックラズベリーはバラ科キイチゴ属に属する。また、本発明のブラックラズベリー抽出物は、主にブラックラズベリーの果実からの抽出物が好適に用いられる。
　ブラックラズベリー抽出物をブラックラズベリーの果実から抽出する方法は、特に限定されないが、溶媒抽出、超臨界抽出等を用いることができる。 Black raspberry which becomes a raw material of a black raspberry extract belongs to Rosaceae raspberry genus. In addition, as the black raspberry extract of the present invention, an extract mainly from black raspberry fruits is preferably used.
The method for extracting black raspberry extract from black raspberry fruits is not particularly limited, but solvent extraction, supercritical extraction and the like can be used.
　溶媒抽出を行う場合に用いる抽出溶媒としては、特に限定されず、水、有機溶媒およびこれらの混合溶媒を用いることができる。有機溶媒としては、メタノール、エタノール、ｎ－プロパノール、イソプロパノール、ｎ－ブタノール等のアルコール類、エチレングリコール、プロピレングリコール等のグリコール類、酢酸エチル、アセトン等を用いることができる。これらの有機溶媒は一種類を単独で用いてもよいし、二種類以上を組み合わせて用いてもよい。また、水等の水系溶媒と有機溶媒とを混合して用いてもよい。 It does not specifically limit as an extraction solvent used when performing solvent extraction, Water, an organic solvent, and these mixed solvents can be used. As the organic solvent, alcohols such as methanol, ethanol, n-propanol, isopropanol and n-butanol, glycols such as ethylene glycol and propylene glycol, ethyl acetate, acetone and the like can be used. One of these organic solvents may be used alone, or two or more of these organic solvents may be used in combination. In addition, an aqueous solvent such as water and an organic solvent may be mixed and used.
　これらの中でも、エタノール、エタノールと水とを混合したエタノール水溶液が好ましく、エタノール水溶液中のエタノールの濃度は、好ましくは１～８０ｖ／ｖ％（ＥｔＯＨ）、より好ましくは１０～６０ｖ／ｖ％（ＥｔＯＨ）、さらに好ましくは２０～４０ｖ／ｖ％（ＥｔＯＨ）である。
　なお、超臨界抽出を行う場合には、超臨界状態とした二酸化炭素を用いることが好ましい。 Among these, ethanol, an aqueous ethanol solution in which ethanol and water are mixed, is preferable, and the concentration of ethanol in the aqueous ethanol solution is preferably 1 to 80 v / v% (EtOH), more preferably 10 to 60 v / v% (EtOH And more preferably 20 to 40 v / v% (EtOH).
When supercritical extraction is performed, it is preferable to use carbon dioxide in a supercritical state.
　また、溶媒抽出を行う場合の抽出方法としては、撹拌抽出、浸漬抽出、振とう抽出、還流抽出、超音波抽出等を用いることができる。これらのなかでも、攪拌抽出が好ましい。
攪拌抽出の方法は、特に限定されないが、撹拌子を用いた攪拌、ブレンダーを用いた撹拌等を行うことができる。 Moreover, stirring extraction, immersion extraction, shaking extraction, reflux extraction, ultrasonic extraction etc. can be used as an extraction method in the case of performing solvent extraction. Among these, stirring extraction is preferable.
The method of stirring and extracting is not particularly limited, but stirring using a stirrer, stirring using a blender, and the like can be performed.
　また、攪拌抽出を行う際は、好ましくは５００～２００００ｒｐｍ、より好ましくは１０００～１００００ｒｐｍ、さらに好ましくは６０００～１００００ｒｐｍの撹拌速度にて攪拌しながら好ましくは数分～６０分間、より好ましくは３０分～６０分間抽出を行う。なお、攪拌後、静置し、静置抽出を行うことが好ましい。より具体的には、ホモジナイザーを用い、６０００～１００００ｒｐｍで３０分～６０分間ホモジナイズを行い、その後２４時間静置し抽出を行うことが好ましい。なお、静置し、抽出を行うことにより、固液分離を行うことができる。 When stirring and extraction is carried out, it is preferably several minutes to 60 minutes, more preferably 30 minutes to 60 minutes while stirring at a stirring speed of preferably 500 to 20000 rpm, more preferably 1000 to 10000 rpm, still more preferably 6000 to 10000 rpm. Extract for 60 minutes. In addition, after stirring, it is preferable to leave still and perform stationary extraction. More specifically, it is preferable to perform homogenization for 30 minutes to 60 minutes at 6000 to 10000 rpm using a homogenizer, followed by standing for 24 hours for extraction. In addition, solid-liquid separation can be performed by leaving still and performing extraction.
　また、溶媒抽出を行う場合の温度は、室温であってもよいし、加熱条件下であってもよい。また、溶媒抽出を行う場合の圧力は常圧であってもよいし、加圧下であってもよい。
　また、溶媒抽出によって得られた抽出液は、必要に応じて濾過等の処理を行ってもよい。 In addition, the temperature for solvent extraction may be room temperature or heating conditions. The pressure in the case of solvent extraction may be normal pressure or may be under pressure.
In addition, the extract obtained by the solvent extraction may be subjected to a treatment such as filtration as necessary.
　また、溶媒抽出によって得られた抽出液を乾燥させて粉末としてもよい。乾燥方法としては、公知の方法を用いることができるが、噴霧乾燥、真空乾燥、凍結乾燥等の手法を用いることができる。
　なお、ブラックラズベリー抽出物としては、液体や粉末状とされた市販品または既製品を用いてもよい。 Alternatively, the extract obtained by solvent extraction may be dried to form a powder. Although a publicly known method can be used as a drying method, methods such as spray drying, vacuum drying, lyophilization and the like can be used.
In addition, as a black raspberry extract, you may use the commercial item made into liquid or powder form, or an existing product.
　本発明のブラックラズベリー抽出物は、食品や飲料等に配合することができる。食品としては、パン類、麺類、菓子類、食肉加工品、魚介加工品、冷凍食品、ゼリー類、アイスクリーム類、乳製品、各種調味料等が挙げられる。また、一般食品の他、特定保健用食品、医薬部外品、健康食品、サプリメントにも配合させることができる。飲料としては、清涼飲料水、乳飲料、酒類、茶、紅茶飲料、コーヒー、果汁飲料、炭酸飲料、ミネラルウォーター類、果実・野菜飲料等が挙げられる。
　また、本発明のブラックラズベリー抽出物を配合させた食品や飲料を、錠剤、カプセル剤、シロップ等の経口投与製剤と同様の形態としてもよい。 The black raspberry extract of the present invention can be blended into foods, beverages and the like. Examples of the food include breads, noodles, confectionery, processed meat products, processed fish products, frozen foods, jellies, ice creams, dairy products, various seasonings and the like. In addition to general foods, it can also be formulated into specified health foods, quasi-drugs, health foods, and supplements. Examples of the beverage include soft drinks, milk drinks, liquors, tea, black tea drinks, coffee, fruit juice drinks, carbonated drinks, mineral waters, fruits and vegetable drinks and the like.
Also, the food or beverage mixed with the black raspberry extract of the present invention may be in the same form as an oral preparation such as tablets, capsules and syrups.
　また、本発明のブラックラズベリー抽出物を配合させた食品や飲料を製造する際に、本発明の効果を妨げない範囲で必要に応じて、甘味料、着色料、保存料、増粘剤、安定化剤、ゲル化剤、酸化防止剤、発色剤、漂白剤、乳化剤、膨張剤、酸味料、光沢剤、香料等の添加剤、溶剤、油を添加してもよい。これらの添加剤は一種類を単独で用いてもよいし、二種類以上を組み合わせて用いてもよい。 In addition, when the food or beverage containing the black raspberry extract of the present invention is produced, a sweetener, a coloring agent, a preservative, a thickener, a stabilizer, as needed, as long as the effects of the present invention are not impaired. Additives, such as additives, gelling agents, antioxidants, color formers, bleaches, emulsifiers, expanders, acidulants, brighteners, perfumes, solvents, oils may be added. One of these additives may be used alone, or two or more thereof may be used in combination.
　上記食品や飲料中に配合されるブラックラズベリー抽出物の割合は、使用目的に応じて適宜調整することができるが、上記食品や飲料中に配合されるブラックラズベリー抽出物の割合は、好ましくは０．０００１～８０重量％、より好ましくは０．００３～５０重量％、さらに好ましくは０．００５～３０重量％である。
　本発明のブラックラズベリー抽出物は、肌の美白効果を得るために有用である。 The proportion of the black raspberry extract incorporated in the above food or beverage can be appropriately adjusted according to the purpose of use, but the proportion of the black raspberry extract incorporated in the above food or beverage is preferably 0. .0001 to 80% by weight, more preferably 0.003 to 50% by weight, and still more preferably 0.005 to 30% by weight.
The black raspberry extract of the present invention is useful for obtaining a skin whitening effect.
　以下に、実施例を挙げて本発明を説明するが、本発明はこれに限定されるものではない。なお、本実施例における部および％は、特記しない限り重量基準である。実施例及び比較例においては、ＯＲＡＣ値による抗酸化作用の評価および、メラノサイトにおけるチロシナーゼ酵素の活性阻害効果について検討を行った。 Hereinafter, the present invention will be described by way of examples, but the present invention is not limited thereto. In the examples, parts and% are by weight unless otherwise specified. In Examples and Comparative Examples, the evaluation of the antioxidant activity by the ORAC value and the activity inhibitory effect of tyrosinase enzyme in melanocytes were examined.
　試料について
　（試料の準備）
　試料としては、ベリー系試料３種（ブラックラズベリー（バラ科キイチゴ属）（以下、「ＢＲ」ということがある。）、ブルーベリー（ツツジ科スノキ属）（以下、「ＢＢ」ということがある。）およびラズベリー（バラ科キイチゴ属）（以下、「ＲＢ」ということがある。））の抽出物、およびブラックラズベリーの抽出物を配合した粉末製剤であるBlabina（登録商標）を用いた。 About sample (preparation of sample)
As a sample, three kinds of berry samples (black raspberry (it is sometimes referred to as "BR") (hereinafter referred to as "BR") and blueberries (hereinafter referred to as "BB") are referred to as blueberry (hereinafter referred to as "BB"). An extract of raspberry (Raspberry raspberry) (hereinafter sometimes referred to as "RB")) and a powder preparation, Blabina (registered trademark), which contains an extract of black raspberry, were used.
　（抽出液の調製）
　ブラックラズベリー、ブルーベリーおよびラズベリーの抽出液は、以下の手法により得た。
　まず、ブラックラズベリー、ブルーベリーおよびラズベリーの果実をそれぞれ冷凍状態にて保存した。次に、それぞれの果実を解凍してカットした。ブラックラズベリー、ブルーベリーおよびラズベリーをそれぞれ秤量（約１００ｇ）し、５倍量の３０ｖ／ｖ％（ＥｔＯＨ）エタノール水溶液（およそ５００ｍＬ）を加え、ブレンダーを用いて８０００ｒｐｍ、３０分の条件にて撹拌抽出を行った。さらに、室温にて２４時間静置することにより、静置抽出を行い、１５０メッシュで濾過して上記ブラックラズベリー、ブルーベリーおよびラズベリーのエタノール抽出液をそれぞれ得た。後述するＯＲＡＣの測定はエタノール溶解状態で行うため、エタノール抽出液を用いて測定を行った。
　なお、Blabinaについては、３０ｖ／ｖ％（ＥｔＯＨ）にBlabinaを９ｍｇ／ｍLとなるように溶解させた。 (Preparation of extract)
Extracts of black raspberry, blueberry and raspberry were obtained by the following procedure.
First, black raspberry, blueberry and raspberry fruits were each stored frozen. Next, each fruit was thawed and cut. Weigh each black raspberry, blueberry and raspberry (about 100 g), add 5 volumes of 30 v / v% (EtOH) aqueous ethanol (approximately 500 mL) and stir extract at 8000 rpm for 30 minutes using a blender went. Furthermore, stationary extraction was performed by leaving still at room temperature for 24 hours, and filtration through 150 mesh was performed to obtain ethanol extracts of the above black raspberry, blueberry and raspberry, respectively. Since measurement of ORAC described later is performed in an ethanol-dissolved state, measurement was performed using an ethanol extract.
In addition, about Blabina, it dissolved so that it might become 9 mg / mL in 30 v / v% (EtOH).
　（抽出物の調製）
　上記抽出液の調製にて得られたブラックラズベリー、ブルーベリーおよびラズベリーそれぞれについてのエタノール抽出液、さらにBlabinaのエタノール溶液から、それぞれエバポレーターを用いてエタノール成分を蒸発させ、除去し、抽出物を得た。なお、ブラックラズベリー、ブルーベリーおよびラズベリーのエタノール抽出液は、９ｍｇ／ｍＬの抽出物（エキス）を含んでいた。 (Preparation of extract)
The ethanol component was removed by evaporation using an evaporator from the ethanol extract of black raspberry, blueberry and raspberry obtained in the above preparation of the extract, and the ethanol solution of Blabina, respectively, to obtain an extract. Furthermore, the ethanol extract of black raspberry, blueberry and raspberry contained 9 mg / mL of extract (extract).
　測定及び評価
　（ＯＲＡＣ値による抗酸化作用の評価）
　上記にて得られたベリー系検体３種（ブラックラズベリー、ブルーベリーおよびラズベリーのエタノール抽出液）のＯＲＡＣ値を測定した。結果を表１に示す。なお、Blabinaについては、ＯＲＡＣ値の測定を行わなかった。 Measurement and evaluation ( evaluation of antioxidative effect by ORAC value)
The ORAC values of three types of berry samples (black raspberry, blueberry and ethanol extract of raspberry) obtained above were measured. The results are shown in Table 1. The ORAC value was not measured for Blatina.
Figure JPOXMLDOC01-appb-T000001
Figure JPOXMLDOC01-appb-T000001
　一般的な抗酸化食品と比較して、ベリー系検体３種のＯＲＡＣ値はいずれも高い値を示した（一般的な抗酸化食品のＯＲＡＣ値については、上記非特許文献４参照）。ベリー系検体３種の中ではブルーベリーが最も高い値を示したが、これはエタノール抽出液を用いて測定した結果であるため、水溶性成分について言及することはできない。 The ORAC values of the three berry samples were all higher than those of the general antioxidant food (see Non-Patent Document 4 above for the ORAC value of the general antioxidant food). Among the 3 types of berry samples, blueberry showed the highest value, but this is a result of measurement using an ethanol extract, so it can not be mentioned about water-soluble ingredients.
　また、エタノール濃度が０．１％程度以上では細胞への影響があるため、細胞を用いた測定および評価を行う場合には、３０ｖｏｌ％エタノール水溶液を用いたエタノール抽出液を用いることは適切ではない。そのため、以下のメラノサイトにおけるチロシナーゼ酵素の活性阻害効果の検討では、それぞれエタノール抽出液から、エバポレーターによってエタノール成分を蒸発除去した検体を用いた（上記「抽出物の調製」参照）。 In addition, since there is an influence on cells when the ethanol concentration is about 0.1% or more, it is not appropriate to use an ethanol extract using a 30 vol% ethanol aqueous solution when performing measurement and evaluation using cells. . Therefore, in the examination of the activity inhibitory effect of the tyrosinase enzyme in the following melanocytes, the specimen which evaporated-removed the ethanol component with the evaporator from each ethanol extract was used (refer the above-mentioned "preparation of an extract").
　（メラノサイトにおけるチロシナーゼ酵素の活性阻害効果）　実験に用いる細胞としては、メラノサイトを用いた。メラノサイトとしては、Normal Human Epidermal Melanocytes, juvenile foreskin（C-12400, PromoCell社より購入）を用い、専用培地（Melanocyte Growth Medium Supplement Pack; C-39410, PromoCell社より購入）を使用して３７℃、５％ＣＯ2の条件にて培養した。実験の直前に０．２５％Trypsin/EDTA溶液で細胞を回収し、細胞数をトリパンブルー染色処理後、Countess（Invitrogen）にて算出した。その後、２４ウェルプレートまたは９６ウェルプレートに播種し、２４時間、前培養したのち実験に用いた。 (Inhibition effect of tyrosinase enzyme in melanocytes) As cells used for experiments, melanocytes were used. For melanocytes, use Normal Human Epidermal Melanocytes, juvenile foreskin (C-12400, purchased from PromoCell) and a dedicated medium (Melanocyte Growth Medium Supplement Pack; C-39410, purchased from PromoCell) at 37 ° C, 5 and cultured in% CO 2 conditions. Immediately before the experiment, the cells were recovered with a 0.25% Trypsin / EDTA solution, and the number of cells was stained with trypan blue and then counted by Countess (Invitrogen). Thereafter, the cells were seeded in a 24-well plate or a 96-well plate, precultured for 24 hours, and then used for experiments.
　また、上記抽出物の調製において得られた３種のベリー系（ブラックラズベリー、ブルーベリーおよびラズベリー）抽出物、Blabinaのエタノール除去抽出物を、それぞれ超純水に溶解させて約１１．５ｍｇ／ｍＬとし、検体を得た。次にそれぞれの検体を段階希釈（１０００００倍希釈から１０倍希釈）し、それぞれメラノサイトに加えた。 In addition, the three berry-based (black raspberry, blueberry and raspberry) extracts obtained in the preparation of the above extract, and the ethanol-removed extract of Blabina are each dissolved in ultrapure water to about 11.5 mg / mL. , Got the sample. Next, each sample was serially diluted (100000-fold dilution to 10-fold dilution) and added to melanocytes, respectively.
　次に、０．１Ｍリン酸バッファー（ｐＨ６．５）６０μＬに、２．５ｍＭ Tyrosine水溶液を３３．３μＬ加えた（２５℃）。そこに、３．３μＬのＬ－チロシン基質溶液を加え撹拌し、３．３μＬのチロシナーゼ水溶液（約１３００ｕｎｉｔ／ｍＬ)を加えて酵素反応を開始させた。プレートリーダー内を２５℃に設定し、酵素反応を行った。 Next, 33.3 μL of 2.5 mM Tyrosine aqueous solution was added to 60 μL of 0.1 M phosphate buffer (pH 6.5) (25 ° C.). Thereto, 3.3 μL of L-tyrosine substrate solution was added and stirred, and 3.3 μL of tyrosinase aqueous solution (about 1300 units / mL) was added to start the enzyme reaction. The inside of the plate reader was set to 25 ° C. to carry out the enzyme reaction.
　酵素活性の評価はチロシナーゼにより合成されるＤＯＰＡクロムの吸光度（４７５nm）を指標とした。なお、Positive controlとしてビタミン－Ｃ　２０ｍｇ／ｍＬを用いた（例えば、上記非特許文献５およびMatsuki M, Watanabe T, Ogasawara A, Mikami T, Matsumoto T. Inhibitory mechanism of melanin synthesis by glutathione. [Article in Japanese] Yakugaku Zasshi. 2008 Aug;128(8):1203-7 12. 参照）。 The evaluation of the enzyme activity was indicated by using the absorbance (475 nm) of DOPA chromium synthesized by tyrosinase. In addition, vitamin-C 20 mg / mL was used as positive control (For example, the said nonpatent literature 5 and Matsuki M, Watanabe T, Ogasawara A, Mikami T, Matsumoto T. Inhibitory mechanism of melanin synthesis by glutathione. [Article in Japanese Yakugaku Zasshi. 2008 Aug; 128 (8): 1203-7 12.).
　結果を図１に示す。ＤＯＰＡクロムの５０％Inhibition（ＩＣ５０）は、概算でpositive control（ビタミン－Ｃ）で１０００００倍希釈、Blabinaで５００００倍希釈、ＢＲで１００００倍希釈、ＢＢで２００倍希釈、ＲＢで１００希釈となった。これはBlabinaおよびＢＲが、ＢＢ及びＲＢに比較して数百倍以上の阻害活性を有することを示すものである。 The results are shown in FIG. 50% Inhibition (IC50) of DOPA chromium was 100 000-fold diluted in positive control (Vitamin-C), 5 000-fold diluted in Blaina, 10000-fold diluted in BR, 200-fold diluted in BB, 100-fold diluted in RB . This indicates that Blabina and BR have several hundred times more inhibitory activity than BB and RB.

Claims (5)

  1. 　メラノサイトにおけるチロシナーゼ酵素の活性阻害効果を有するブラックラズベリー抽出物。 Black raspberry extract having the activity inhibitory effect of tyrosinase enzyme in melanocytes.
  2. 　請求項１に記載のブラックラズベリー抽出物を含む美白剤。 A whitening agent comprising the black raspberry extract according to claim 1.
  3. 　請求項１に記載のブラックラズベリー抽出物を含む飲食物。 Food and drink containing the black raspberry extract according to claim 1.
  4. 　請求項１に記載のブラックラズベリー抽出物の製造方法であって、
    　ブラックラズベリーの果実を冷凍する冷凍工程と、
    　前記ブラックラズベリーの果実を解凍する解凍工程と、
    　前記解凍工程にて解凍した前記ブラックラズベリーの果実に溶媒を加えて撹拌する撹拌工程と、
    　前記撹拌工程により撹拌した前記ブラックラズベリーの果実および溶媒を静置し抽出を行う抽出工程と
    を含むブラックラズベリー抽出物の製造方法。     A method for producing a black raspberry extract according to claim 1,
    Freezing the fruit of black raspberry, and
    A thawing step of thawing said black raspberry fruit;
    A stirring step of adding a solvent to the black raspberry fruit thawed in the thawing step and stirring;
    A process of producing a black raspberry extract comprising an extraction process in which the black raspberry fruit and the solvent stirred in the stirring process are allowed to stand and extract.
  5. 　請求項１に記載のブラックラズベリー抽出物を用いた美白方法。 A whitening method using the black raspberry extract according to claim 1.
PCT/JP2018/006356 2017-08-09 2018-02-22 Black raspberry extract, skin lightening agent and food or beverage product including black raspberry extract, production method for black raspberry extract, and skin lightening method using black raspberry extract WO2019030956A1 (en)

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