JP2002161042A - Antioxidant - Google Patents

Abstract

PROBLEM TO BE SOLVED: To obtain an antioxidant having an excellent active oxygen scavenging ability and safety. SOLUTION: This antioxidant comprises a leaf or its extract of Cinnamomum camphora Sieb. glaucescens Alex. Braun belonging to the genus Cinnamomum of the family Lauraceae as an active ingredient. Thereby, a new quality retaining agent for medicines, foods, cosmetics, etc., and an in vivo free radical scavenger can be provided.

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an antioxidant containing camphor leaf or an extract thereof as an active ingredient, which can be used for pharmaceuticals, foods, feeds, cosmetics and the like.

[0002]

2. Description of the Related Art Generally, food is produced from agricultural products, marine products, livestock products, and the like. However, food raw materials and products, especially those containing unsaturated fatty acids, are susceptible to rancidity due to oxidation or peroxidation by oxygen in the air during the manufacturing process including heating or during storage, and some kind of oxidation prevention Agent is used.

As such an antioxidant, butylhydroxytoluene (BHT) and butylhydroxyanisole (BHA), which are synthetic products, have been used in many cases, but recently there has been a suspicion of carcinogenicity. , Its use has been reviewed.

[0004] On the other hand, it is considered that peroxides and carcinogens generated in the living body of animals due to the toxic effect of oxygen have a bad effect on animal cells. It is thought to cause cell aging, and thus shorten the life of an individual (free radical aging theory). Therefore, naturally occurring antioxidants with high safety are expected to support antioxidant defense mechanisms in vivo in foods, especially health foods and nutritional foods, as well as in the pharmaceutical and cosmetic technical fields. Have been.

[0005] Therefore, many attempts have been made to obtain highly safe and sufficiently effective antioxidants from natural products in place of synthetic antioxidants, and some of them have been put to practical use. For example, currently, natural products derived antioxidants used in food processing and the like, tocopherols contained in various vegetable oils, flavone contained in various plant tissues, quercetin,
Flavone derivatives such as rutin, caffeic acid contained in tea leaves, coffee beans, cocoa beans, etc., gallic acid derivatives such as ferulic acid and polyphenols such as catechins,
Gossypol contained in cottonseed oil, γ-oryzanol in rice bran, sesamol / sesamolin in sesame oil, extracts of various spices, protein hydrolysates (peptides, amino acids)
(For example, JP-A-59-45385).
JP-A-59-166585, JP-A-60-365
No. 84, JP-B-61-34792, JP-A-3-756.
However, they also do not satisfy all of the antioxidant activity, convenience and safety.

[0006] On the other hand, the camphor is a camphor tree (Lauraceae).
ae) It belongs to the genus camphor (Cinnamomum), mainly distributed from southern China to southeastern Taiwan, and is also cultivated in Japan. It contains 30-70% linalool, and its essential oil is mainly used for fragrance applications. However,
Other uses have not been known so far, and only a slight use as a tea beverage has been proposed (Japanese Patent Application Laid-Open No.
0-84922).

[0007]

An object of the present invention is to provide a new natural antioxidant having an antioxidant effect on active oxygen which has a harmful effect on foods, feeds, cosmetics, pharmaceuticals and living bodies. Make it an issue.

[0008]

Means for Solving the Problems The present inventors have conducted intensive studies in order to solve the above-mentioned problems, and as a result, have found that camphor (Cin)
namum camphora Sieb. gl
aussensAlex. For the first time, the present inventors found that excellent antioxidant properties were present in the leaves of Braun) and completed the present invention.

That is, the present invention provides an antioxidant characterized by containing camphor leaf or an extract thereof as an active ingredient.

[0010]

BEST MODE FOR CARRYING OUT THE INVENTION The antioxidant referred to in the present invention is an antioxidant as a quality preserving agent used for pharmaceuticals, foods, feeds, cosmetics, etc., or has a function as a radical scavenger in vivo. In addition, it is characterized by containing a camphor leaf or an extract thereof as an active ingredient.

The camphor leaf used in the present invention is not particularly limited, such as the place of production and the time of plucking, but is preferably a young leaf. The used camphor leaf may be raw or dried, or may be a residue after collecting essential oil. In the present invention, camphor leaf or its dried product may be used as it is or after being processed into an arbitrary form, but it is preferable to use an extract because it can be used for a wider range of uses and is highly effective.

In the present invention, when camphor leaves are used raw, they may be used as they are, or may be cut and used or crushed and used in a slurry form depending on the use. When dried camphor leaves are used, they can be dried by a known method such as shade drying, air drying, hot air drying, and freeze drying. The dried camphor leaf may be used as it is, but may be formed into chips, or may be used after being ground or ground. The shape of the camphor leaves used for the extraction is not particularly limited, but it is preferable to use dried leaves cut or crushed to 5 mm or less in consideration of the extraction efficiency and the simplicity of the operation.

The camphor leaf extract of the present invention can be extracted by any method such as heat extraction, continuous extraction, immersion extraction, countercurrent extraction, and supercritical extraction, but is preferably extracted using a polar solvent. . Examples of polar solvents include alcohols such as methanol, ethanol, and butanol; acetone, ethyl acetate, and organic solvents such as diethyl ether;
Water may be used, and a plurality of these solvents may be used in combination. However, hydrous ethanol is particularly preferably used from the viewpoint of safety in use in foods.

In the present invention, when a polar solvent is extracted,
It is preferable to use 5 to 100 parts by weight of a polar solvent per 1 part by weight of camphor leaf. For example, when using hydrated ethanol, the amount of hydrated ethanol is more preferably 10 to 50 parts by weight. In addition, water and ethanol in hydrous ethanol can be mixed at an arbitrary ratio.
The ratio is more preferably 0:10 to 40:60.

The extraction temperature is not particularly limited, but is preferably from 10 to 80 ° C, more preferably from 20 to 60 ° C. The extraction time is not particularly limited, but is preferably several minutes to 2 hours, and more preferably 10 to 60 minutes with stirring and extraction.

In the present invention, the extract extracted from the camphor leaves may be used as an aqueous solution or solid by removing the polar solvent, if necessary. When the extract is dried to form a solid, an excipient such as dextrin can be added, and further, drying can be performed by a known method such as spray drying or freeze drying. The obtained solid may be pulverized or ground to a powdery form, and further, may be formed into a tablet, a tablet or the like by a known method.

The antioxidant of the present invention can be added to pharmaceuticals, foods, feeds, and cosmetics as a quality preservative or an active oxygen scavenger. For example, in food, coffee, tea, tea, fruit juice, beverages such as soft drinks, milk,
Dairy products such as butter, cheese and cream, processed foods such as miso soup and soup, edible fats and oils, processed fats and oils such as margarine, seasonings such as mayonnaise and dressing,
Other nutritional supplements, liquid or fluid foods such as syrups and jams, processed grain products such as cooked rice, rice cakes, bread, potato products, confectionery products such as chocolate, candy, baked confectionery, processed meat products such as ham and sausage, In addition, it can be added to solid foods such as sprinkles. In pharmaceuticals, for example, oral administration, suppositories,
It can be used for coatings, ointments, patches, baths, eye drops, and injections. In cosmetics, for example, lotions,
Emulsions, creams, packs, skin cleansers, haptics, pastes, essences, gels, shampoos, rinses,
It can be used for powders, foundations, lotions, dyes and pigments, hair tonics, hair tonics, baths, whitening agents, UV care products and the like.

It is also possible to use a plurality of other antioxidants, for example, a commercially available antioxidant such as tocopherol, ascorbic acid and sodium erythorbate in combination. The ratio of camphor extract to commercial antioxidant when used together is 10:90
-90: 10 is preferable, and 50: 50-80: 20 is more preferable.

(Example 1) Production of antioxidant from camphor leaf 20 g of leaf of 8 camphor leaves or young leaves air-dried for 1 week
160 g of 0,99 v / v% ethanol was added,
For 1 hour and filtered. The obtained filtrate was separated by centrifugation, and the solvent was distilled off from the supernatant under reduced pressure to obtain an extract.

Example 2 Measurement of DPPH Radical Scavenging Activity of Camphor Leaf Extract DPPH (2,2-diphenyl-1-picry)
lhydrazyl) is a relatively stable radical and is widely used for screening for radical scavenging activity. The radical scavenging activity of the antioxidant obtained by the present invention was measured by the following method.

2 mL of a 250 ppm ethanol solution of the extract obtained in Example 1, 0.1 M acetate buffer (pH 5.
5) 1m of 0.5mM DPPH ethanol solution in 2mL
L was added to make the total amount 5 mL, and the mixture was stirred well. 3 at room temperature
After standing for 0 minutes, the absorbance at 517 nm was measured. As a control, measurement was performed by adding only ethanol instead of the sample. The DPPH radical scavenging rate was calculated by the following equation, and this was defined as DPPH radical scavenging activity. The results are shown in Table 1. DPPH radical scavenging rate (%) = {1− (absorbance of sample-added group / absorbance of control group)} × 100

[0022]

[Table 1]

As is evident from the results in Table 1, the 50% ethanol extract exhibited the highest radical scavenging activity.
In addition, no matter which ratio of aqueous ethanol was used for extraction, the young leaves showed higher activity.

(Example 3) Production of antioxidant from camphor leaf extract In Example 2, a 50% ethanol extract having the highest radical scavenging activity was subjected to solvent fractionation.

In the same manner as in Example 1, 2.46 g of a 50% ethanol extract was obtained using 16 g of camphor leaf (evergreen). Using 2.46 g of this extract, the solvent was fractionated with hexane / water. The obtained aqueous layer was further fractionated with ethyl acetate and a solvent,
Hexane-soluble fraction 0.06 g, ethyl acetate-soluble fraction 0.43 g, and water-soluble fraction 1.43 g were obtained as three fractions.

(Example 4) Measurement of DPPH radical scavenging activity of the solvent fraction The DPPH radical scavenging activity of the solvent fraction obtained in Example 3 was measured in the same manner as in Example 2.

[0027]

[Table 2]

As is clear from the results in Table 2, high radical scavenging activity was observed in the ethyl acetate-soluble fraction and the water-soluble fraction. These activities were stronger than BHT.

Example 5 Measurement of Free Radical Scavenging Activity of Camphor Leaf Since an electron spin resonance apparatus (ESR) can detect radicals, it is used for measuring active oxygen species. The antioxidant scavenging activity of the antioxidant obtained by the present invention was measured using the following method.

Dimethyl sulfoxide (DMSO) 50μ
L, 50 μL of 25 mM sodium hydroxide solution, 50 μL of 30% aqueous hydrogen peroxide, 5 μL of dimethylpyrroline N-oxide (DMPO), 20% ethanol extract of camphor leaf or β-carotene (Car), ascorbic acid (As)
A), 50 μL of a 1% solution of tocopherol (Toc) was added, and the ESR was measured as a total volume of 205 μL. ES
The elimination rate of each free radical was determined from the signal intensity of R. The results are shown in Table 3.

[0031]

[Table 3]

From the results shown in Table 2, the camphor leaf extract had high free radical scavenging activity. In particular, it had strong activity on superoxide and hydroxyl.
In addition, the negative value of ascorbic acid is considered to be due to the fact that the methyl radical was easily generated in this system as the ascorbic acid radical was generated.

(Example 6) Measurement of lipid peroxidation effect of camphor leaf The Rancimat method can well express the protective power of lipid against the action of oxygen. The lipid peroxidation inhibitory effect of the antioxidant obtained by the present invention was measured by comparing with a commercially available antioxidant using the following method.

To 3 g of lard, BH was added as a commercial antioxidant.
A, 200 ppm of ascorbic acid or KPA-F (trade name: grape seed extract, manufactured by Kikkoman) was added.
In addition, a 50% ethanol extract of camphor leaf was added in an amount corresponding to 200 ppm equivalent of ascorbic acid from Example 2,
The temperature was measured by heating to 120 ° C. In addition, it measured without adding an antioxidant as a control. The effect of inhibiting lipid peroxidation was expressed as the induction time of the peroxidation reaction. The results are shown in Table 4.

[0035]

[Table 4]

From the results in Table 3, it can be seen that the fat-soluble antioxidant B
Although the activity was inferior to that of HA, it showed almost the same activity as ascorbic acid which is a water-soluble antioxidant, and showed higher activity than KPA-F.

Example 7 Soft drink: 20 parts of orange juice, 15 parts of sucrose, 0.2 part of citric acid
Parts, 0.1 part of the 50% ethanol extract obtained in Example 1,
0.1 part of a fragrance and 64.6 parts of water were added, and the mixture was sterilized by heating at 80 ° C. for 30 minutes, and then cooled to obtain a soft drink.

[0038]

The antioxidant of the present invention is obtained from natural camphor leaves and has high safety and strong antioxidant activity, especially free radical scavenging activity. It is useful for stabilizing pharmaceuticals, foods, cosmetics, etc. and for scavenging free radicals in living bodies.

Continuation of the front page (72) Inventor Tatsuya Umeda 1424, Kamigongo, Matsudo-shi, Chiba Prefecture F-term in TUC (reference) 4C083 AA111 BB47 CC04 CC05 CC07 CC12 CC19 CC23 CC25 CC37 CC38 CC39 DD12 DD17 DD41 EE12 EE16 EE17 FF01 4C088 AB33 AC05 BA08 CA06 CA07 CA08 CA09 NA05 NA06 NA07 ZC37 ZC41

Claims (4)

[Claims] 1. An antioxidant comprising camphor leaf as an active ingredient. 2. An antioxidant comprising a camphor leaf extract as an active ingredient. 3. The antioxidant according to claim 2, wherein the active ingredient is a polar solvent extract or a purified product thereof of fresh leaves, dried leaves or residues of essential oil after collecting essential oil. 4. A pharmaceutical, food, feed, or cosmetic comprising the antioxidant according to claim 1.