WO2019022578A3 - 식물 유래 dna 탈메틸효소를 이용한 동물세포에서의 인터페론의 발현 수준의 증가용 조성물, 항바이러스용 조성물, 및 이를 이용한 방법 - Google Patents

식물 유래 dna 탈메틸효소를 이용한 동물세포에서의 인터페론의 발현 수준의 증가용 조성물, 항바이러스용 조성물, 및 이를 이용한 방법 Download PDF

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WO2019022578A3
WO2019022578A3 PCT/KR2018/008580 KR2018008580W WO2019022578A3 WO 2019022578 A3 WO2019022578 A3 WO 2019022578A3 KR 2018008580 W KR2018008580 W KR 2018008580W WO 2019022578 A3 WO2019022578 A3 WO 2019022578A3
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composition
animal cells
interferon
expression level
same
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PCT/KR2018/008580
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English (en)
French (fr)
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WO2019022578A2 (ko
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허진회
목영근
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서울대학교산학협력단
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Priority to US16/495,559 priority Critical patent/US20200017876A1/en
Publication of WO2019022578A2 publication Critical patent/WO2019022578A2/ko
Publication of WO2019022578A3 publication Critical patent/WO2019022578A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/56—IFN-alpha
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/565—IFN-beta
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52—Cytokines; Lymphokines; Interferons
    • C07K14/555—Interferons [IFN]
    • C07K14/57—IFN-gamma
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Plant Substances (AREA)

Abstract

동물 세포에서 인터페론의 발현 수준을 증가시키기 위한 조성물 또는 키트, 항바이러스용 조성물, 및 이들을 이용한 동물 세포에서 인터페론의 발현 수준을 증가시키는 방법을 제공한다. 이에 의하면, 동물세포에서 DME의 발현을 유도함으로써 세포 분열 없이도 IFNβ의 발현 증가에 의해 빠른 시간 내에 항바이러스 반응을 유도할 수 있다.
PCT/KR2018/008580 2017-07-28 2018-07-27 식물 유래 dna 탈메틸효소를 이용한 동물세포에서의 인터페론의 발현 수준의 증가용 조성물, 항바이러스용 조성물, 및 이를 이용한 방법 WO2019022578A2 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US16/495,559 US20200017876A1 (en) 2017-07-28 2018-07-27 Composition for increasing expression level of interferon in animal cells using plant-derived dna demethylase, antiviral composition, and method using same

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0096227 2017-07-28
KR20170096227 2017-07-28

Publications (2)

Publication Number Publication Date
WO2019022578A2 WO2019022578A2 (ko) 2019-01-31
WO2019022578A3 true WO2019022578A3 (ko) 2019-04-11

Family

ID=65040651

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/008580 WO2019022578A2 (ko) 2017-07-28 2018-07-27 식물 유래 dna 탈메틸효소를 이용한 동물세포에서의 인터페론의 발현 수준의 증가용 조성물, 항바이러스용 조성물, 및 이를 이용한 방법

Country Status (3)

Country Link
US (1) US20200017876A1 (ko)
KR (1) KR102096526B1 (ko)
WO (1) WO2019022578A2 (ko)

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001522607A (ja) * 1997-11-12 2001-11-20 マクギル・ユニヴァーシティ Ｄｎａデメチラーゼおよびその治療および診断用途
US20030135890A1 (en) * 2000-04-21 2003-07-17 Robert Fischer Nucleic acids that control plant development
US8951769B2 (en) * 2009-04-20 2015-02-10 The Regents Of The University Of California Engineered demeter 5-methylcytosine DNA glyosylase with improved yield, stability and solubility

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001522607A (ja) * 1997-11-12 2001-11-20 マクギル・ユニヴァーシティ Ｄｎａデメチラーゼおよびその治療および診断用途
US20030135890A1 (en) * 2000-04-21 2003-07-17 Robert Fischer Nucleic acids that control plant development
US8951769B2 (en) * 2009-04-20 2015-02-10 The Regents Of The University Of California Engineered demeter 5-methylcytosine DNA glyosylase with improved yield, stability and solubility

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HEO, JIN HOE: "Study of DNA Demethylation System in Plants and Development of Next Generation Methylome Analysis Tools", RDA (RURAL DEVELOPMENT ADMINISTRATION) NEXT GENERATION BIOGREEN21 FINAL REPORT, 2015 *
MOK, YOUNG GEUN ET AL.: "DEMETER Plant DNA Demethylase Induces Antiviral Response by Interferon Signalling in Animal Cells", SCIENTIFIC REPORTS, vol. 7, no. 9160, 22 August 2017 (2017-08-22), pages 1 - 12, XP055590018 *
PARRILLA-DOBLAS, JARA TERESA ET AL.: "Targeted DNA Demethylation in Human Cells by Fusion of a Plant 5-methylcytosine DNA Glycosylase to a Sequence-specific DNA Binding Domain", EPIGENETICS, vol. 12, no. 4, 30 March 2017 (2017-03-30), pages 296 - 303, XP055590014 *

Also Published As

Publication number Publication date
WO2019022578A2 (ko) 2019-01-31
KR20190013626A (ko) 2019-02-11
US20200017876A1 (en) 2020-01-16
KR102096526B1 (ko) 2020-04-02

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