WO2019022393A3 - Micropuce de culture cellulaire tridimensionnelle ayant une membrane nanoporeuse perméable fixée à celle-ci et procédé de fabrication associé - Google Patents

Micropuce de culture cellulaire tridimensionnelle ayant une membrane nanoporeuse perméable fixée à celle-ci et procédé de fabrication associé Download PDF

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Publication number
WO2019022393A3
WO2019022393A3 PCT/KR2018/007457 KR2018007457W WO2019022393A3 WO 2019022393 A3 WO2019022393 A3 WO 2019022393A3 KR 2018007457 W KR2018007457 W KR 2018007457W WO 2019022393 A3 WO2019022393 A3 WO 2019022393A3
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WO
WIPO (PCT)
Prior art keywords
permeable membrane
microchip
method therefor
fabrication method
cell culturing
Prior art date
Application number
PCT/KR2018/007457
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English (en)
Korean (ko)
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WO2019022393A9 (fr
WO2019022393A2 (fr
Inventor
이광호
이윤경
Original Assignee
강원대학교산학협력단
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Publication of WO2019022393A2 publication Critical patent/WO2019022393A2/fr
Publication of WO2019022393A3 publication Critical patent/WO2019022393A3/fr
Publication of WO2019022393A9 publication Critical patent/WO2019022393A9/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D71/00—Semi-permeable membranes for separation processes or apparatus characterised by the material; Manufacturing processes specially adapted therefor
    • B01D71/06—Organic material
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/12—Apparatus for enzymology or microbiology with sterilisation, filtration or dialysis means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/06—Tissue, human, animal or plant cell, or virus culture apparatus with filtration, ultrafiltration, inverse osmosis or dialysis means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0896—Nanoscaled

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Fluid Mechanics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Water Supply & Treatment (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

La présente invention concerne une micropuce de culture cellulaire tridimensionnelle ayant une membrane perméable nanoporeuse fixée à celle-ci et un procédé de fabrication associé. La présente invention peut cultiver des cellules d'une manière tridimensionnelle avec la membrane perméable nanoporeuse servant d'échafaudage cellulaire et peut injecter des cellules au moyen d'une pluralité de canaux microfluidiques ou fournir un médicament, une substance sécrétée par des cellules, ou un matériau biologiquement actif par diffusion.
PCT/KR2018/007457 2017-07-26 2018-07-02 Micropuce de culture cellulaire tridimensionnelle ayant une membrane nanoporeuse perméable fixée à celle-ci et procédé de fabrication associé WO2019022393A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020170094503A KR101985311B1 (ko) 2017-07-26 2017-07-26 나노다공성 투과막이 부착된 3차원 세포배양용 마이크로 칩 및 그의 제조방법
KR10-2017-0094503 2017-07-26

Publications (3)

Publication Number Publication Date
WO2019022393A2 WO2019022393A2 (fr) 2019-01-31
WO2019022393A3 true WO2019022393A3 (fr) 2019-03-28
WO2019022393A9 WO2019022393A9 (fr) 2019-04-18

Family

ID=65041277

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2018/007457 WO2019022393A2 (fr) 2017-07-26 2018-07-02 Micropuce de culture cellulaire tridimensionnelle ayant une membrane nanoporeuse perméable fixée à celle-ci et procédé de fabrication associé

Country Status (2)

Country Link
KR (1) KR101985311B1 (fr)
WO (1) WO2019022393A2 (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102277296B1 (ko) 2019-11-14 2021-07-15 주식회사 엔케이 저장용기 지지장치
CN112375681B (zh) * 2020-11-19 2022-06-07 中国科学院上海微系统与信息技术研究所 一种器官芯片及其应用
CN114437932B (zh) * 2022-02-14 2023-06-30 重庆医科大学附属第二医院 多胎胎盘血管吻合模型的微流控芯片、系统及使用方法
KR20240006817A (ko) 2022-07-07 2024-01-16 김학균 전방 시야 확보를 위한 차량용 a 필러 및 그를 구비하는 자동차

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006052223A1 (fr) * 2004-11-11 2006-05-18 Agency For Science, Technology And Research Dispositif de culture de cellules
KR20070033685A (ko) * 2005-09-22 2007-03-27 한국과학기술원 미세유체 기술을 이용한 3차원 세포배양 시스템
KR20110044226A (ko) * 2008-07-16 2011-04-28 칠드런'즈 메디컬 센터 코포레이션 마이크로채널을 갖는 기관 모방 장치 및 그 사용 및 제조 방법
KR20130131326A (ko) * 2010-09-29 2013-12-03 메사추세츠 인스티튜트 오브 테크놀로지 세포 상호작용의 고 처리량 조사를 위한 장치
KR20170056241A (ko) * 2015-11-13 2017-05-23 고려대학교 산학협력단 세포배양 칩 및 생성방법

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101458425B1 (ko) 2014-04-03 2014-11-07 동아대학교 산학협력단 3차원 구조의 다단 세포배양 구조체, 이를 포함하는 에세이칩 및 에세이칩을 이용한 세포 분석방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006052223A1 (fr) * 2004-11-11 2006-05-18 Agency For Science, Technology And Research Dispositif de culture de cellules
KR20070033685A (ko) * 2005-09-22 2007-03-27 한국과학기술원 미세유체 기술을 이용한 3차원 세포배양 시스템
KR20110044226A (ko) * 2008-07-16 2011-04-28 칠드런'즈 메디컬 센터 코포레이션 마이크로채널을 갖는 기관 모방 장치 및 그 사용 및 제조 방법
KR20130131326A (ko) * 2010-09-29 2013-12-03 메사추세츠 인스티튜트 오브 테크놀로지 세포 상호작용의 고 처리량 조사를 위한 장치
KR20170056241A (ko) * 2015-11-13 2017-05-23 고려대학교 산학협력단 세포배양 칩 및 생성방법

Also Published As

Publication number Publication date
KR101985311B1 (ko) 2019-06-03
KR20190011894A (ko) 2019-02-08
WO2019022393A9 (fr) 2019-04-18
WO2019022393A2 (fr) 2019-01-31

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