WO2018236177A2 - Preservative solution for bio-cellulose sheet and method for preserving bio-cellulose sheet using same - Google Patents

Abstract

Provided are a preservative solution for a bio-cellulose sheet comprising a catalyst-substituted zeolite having a size of 1 to 990 nm and an aqueous solvent, and a method for preserving a bio-cellulosic sheet using the same. When using the preservative solution of the present invention, the bio-cellulose sheet can be stored for a long period of time. In addition, the preservative solution contains no antiseptic, and thus is harmless to the human body, and the preservative solution can effectively inhibit bacterial growth. DRAWING: FIG. 4C: AA Candida albicans (ATCC 10231) BB 21 day_Candida albicans_<10 CC 28 day_Candida albicans_<10

Description

Storing liquid for bio-cellulose sheet and method for preserving bio-cellulose sheet using the same

The present invention relates to a preservative for a bio-cellulose sheet and a method for preserving the bio-cellulose sheet using the same.

In the field of cosmetics, a sheet-type mask pack has a supporter capable of supporting the emulsion for a long time to act on the skin as compared with general cosmetic lotion, and the skin efficacy component Is a form of cosmetic product that is excellent in moisturizing effect and skin care effect due to promotion of skin penetration effect. Of these sheets of cosmetic mask packs, bio-cellulose sheets are known.

The bio-cellulosic sheet has the problem of contamination and corruption even after washing and sterilization processes due to the nature of cellulose derived from microorganisms. Accordingly, to solve such a problem, the produced bio-cellulose is subjected to a preservative treatment or a preservative liquid treatment to prevent contamination and decay. However, the conventional preservative treatment and preservative liquid treatment are required to be improved because skin irritation, cell mutation, accumulation in the human body, harmful effect, or prevention of contamination and decay are not sufficient.

Disclosure of Invention Technical Problem [8] The present invention provides a preserving solution for a bio-cellulose sheet and a method for preserving a bio-cellulose sheet using the same.

In order to accomplish the object of the present invention, there is provided a storage solution for a bio-cellulosic sheet comprising a catalyst-substituted zeolite having a size of 1 to 990 nm and an aqueous solvent.

According to another aspect of the present invention, there is provided a process for preparing a catalyst-substituted zeolite-containing zeolite, comprising the steps of: (1) adding a catalytic-substituted zeolite to water and stirring the mixture to obtain a catalyst-substituted zeolite aqueous solution containing catalytically substituted zeolite having a particle size of 1 nm to 990 nm;

A second step of preparing a preservative solution for a bio-cellulose sheet using the catalyst-substituted zeolite aqueous solution; And

And a third step of filling the bio-cellulose sheet with the preserving solution for the bio-cellulose sheet.

The aqueous catalyst-substituted zeolite solution may be prepared by: preparing a catalyst-substituted zeolite catalyst by substituting a zeolite powder with a metal-substituted catalyst; Replacing the catalyst-displacing zeolite catalyst by nanofiber processing to produce a liquid catalyst-substituted zeolite having a catalyst-substituted zeolite having a particle size of 1 nm to 990 nm; And diluting the catalyst-substituted zeolite with water to prepare a nanocatalyst-substituted zeolite aqueous solution. The catalyst-substituted zeolite may be at least one selected from the group consisting of Ag, Cu, Fe, Co, Mn, Zn, Ti, Mg, ), Palladium (Pd), zirconium (Zr), and rhodium (Rh).

By using the preserving solution for a bio-cellulose sheet according to one embodiment, the bio-cellulose sheet can be stored for a long period of time, and the preservation performance is excellent.

FIG. 1 is a view for explaining a process for producing a catalyst-substituted zeolite.

2 is a block diagram showing each component in the step of preparing a liquid catalyst-substituted zeolite by atomizing the catalyst-substituted zeolite.

FIGS. 3A to 3C are optical microscope photographs showing the result of a test for confirming the flotation force against Aspergillus niger ATCC 16404 of a mask pack prepared according to Example 1. FIG.

4A to 4C are optical microscope photographs showing the result of a test for confirming the flushing force against Candida albicans (ATCC 10231) in the mask pack prepared in Example 1. FIG.

FIGS. 5A to 5C are optical microscope photographs showing the result of a test for confirming the flotation resistance of Escherichia coli (ATCC 8739) of a mask pack prepared according to Example 1 against a bacterial solution.

6A to 6C are optical microscope photographs showing the results of a test for confirming the flushing force against the mycelial liquid of Pseudomonas aeruginosa (ATCC 9027) of the mask pack prepared according to Example 1. Fig.

FIGS. 7A to 7C are optical microscope photographs showing the test results of the flotation force against the mycelial liquid of Staphlyococcus aureus (ATCC 6538) of the mask pack prepared according to Example 1. FIG.

FIG. 8 is a diagram showing a criterion for determining the degree of stimulation as a result of the skin stimulation test conducted according to the evaluation example 3. FIG.

Figs. 9 and 10 show the results of the skin irritation test conducted according to the evaluation example 2. Fig.

Hereinafter, an exemplary storage solution for a bio-cellulose sheet and a method for preserving a bio-cellulose sheet using the same will be described in detail with reference to the accompanying drawings, and a mask pack and a manufacturing method thereof using the bio-cellulose sheet will be described in detail.

Because bio-cellulose is a natural material, it has no side effects and does not cause environmental pollution. In particular, the bio-cellulose produced by the acetic acid bacteria is produced in a pure state containing no lignan or hemicellulose, unlike the plant-derived cellulose comprising a bundle of fine fibers. Unlike plant celluloses, biocellulose is an ultrafine fiber and has attracted attention as an industrial material for foods and medical care due to its excellent physical strength, innocuous biodegradability, high functional capacity and transparency. Biocellulose is prepared by inoculating microorganisms into a production medium, preferably a fruit juice containing acetic acid and sugar, and cultivating the microorganism in a fermenter in a shaking volume so that microbial cellulose in the form of particles or pellets is produced in a dispersed form do. The culture medium for the microorganism as the production site is not limited to any culture liquid containing nutrients necessary for cultivation such as coconut, apple, or mandarin. In one embodiment of the present invention, coconut extract is used, further comprising sucrose and ammonium sulfate. Preferably, sucrose and ammonium sulfate are added to the coconut solution in an amount of 5 to 15% by weight and 0.5 to 1.5% by weight, respectively, and the mixture is adjusted to pH 4 to 5 with acetic acid and stirred.

The microorganisms used in the culture of bio-cellulose may be microorganisms belonging to various classes. The microorganism producing the bio-cellulose may be any one selected from the group consisting of Acetobacter, Rhizobium and Agrobacterium. Alternatively, various microorganisms may be mixed and cultured for production efficiency . Among them, it is effective to cultivate mixed culture or stirred mixture of lactic acid bacteria producing bacteriocin to prevent bacterial contamination and xylitanium in acetobacterium with good production yield.

According to one embodiment of the present invention, the bio-cellulose is produced using acetobactor xylenol. The culture conditions of the microorganism can be cultured at 20 to 40 ° C. for 3 to 15 days in order to maintain the optimal growth state of the microorganism producing the bio-cellulose.

Since the bio-cellulose of the present invention is easily contaminated and decayed due to external contaminants, the bio-cellulose of the present invention is subjected to a process of destroying the injected cells, removing the culture liquid and filling the preservative solution for use as a cosmetic mask pack sheet. In one embodiment, the generated bio-cellulose is washed with purified water three times or more, compressed to remove water, and sprayed with a preservative solution. It is preferable that the preserving liquid has a antibacterial action and is non-toxic to the human body.

The preservative solution for the bio-cellulosic sheet of the present invention includes a catalyst-substituted zeolite having a size of 1 to 990 nm and an aqueous solvent.

The catalyst-substituted zeolite has a size of 1 to 900 nm, for example, a size of 3 to 600 nm, and is mixed with an aqueous solvent to form an aqueous solution with a catalyst-substituted zeolite. Water-based solvents are water or mixtures of water and alcohol-based solvents.

As used herein, the term &quot; size &quot; refers to the diameter when the catalyst-substituted zeolite is spherical and the longest length when the catalyst-substituted zeolite is non-spherical. When the size of the genus zeolite is in the above range, the preservative effect is excellent. In this specification, the catalyst refers to a metal-substituted catalyst.

The water content in the preservative for the bio-cellulose sheet is 500-5000 parts by weight based on 1 part by weight of the catalyst-substituted zeolite. Examples of the catalyst-substituted zeolite include silver (Ag), copper (Cu), iron (Fe), cobalt (Co), manganese (Mn), zinc (Zn), titanium (Ti), magnesium (Mg) ), Palladium (Pd), zirconium (Zr) and rhodium (Rh). Catalyst substituted zeolites are, for example, zinc zeolites.

It is preferable that the zeolite has a pro-ves guide crystal structure. The probest structure may have a structure in which a diamond crystal surrounds a cubic crystal and a net crystal surrounds the cubic crystal. Having the crystal structure has a better preservation ability.

The catalyst-substituted zeolite can be prepared by dipping the zeolite powder into a liquid metal catalyst. In this step, the catalyst-substituted zeolite catalyst may be firstly pulverized. When the catalyst-substituted zeolite catalyst is pulverized, the catalyst-substituted zeolite catalyst has a high viscosity. When the catalyst-substituted zeolite catalyst having a higher viscosity is pulverized with stirring, a more uniform catalyst-substituted zeolite catalyst is produced.

The catalyst-substituted zeolite catalyst is preferably aged for a certain period of time. The degree of substitution of the liquid metal catalyst 120 with the zeolite powder 110 is improved by the above aging (see FIG. 1).

Thereafter, the catalytic-displacement-type zeolite catalyst 100 is subjected to an atomization process to produce a liquid zeolite having a particle size of 3 nm to 600 nm. That is, when the catalyst-substituted zeolite catalyst is finely processed to have a particle size of 3 nm to 600 nm, the particle size in contact with the periphery becomes large, and the preservation effect is maximized.

To this end, the catalyst-displacement type zeolite catalyst 100 is first mixed with the crushing material 60 in the storage tank 210. The crushing material 60 may be titanium, diamond, or the like, and the size thereof may be 0.05 mm to 0.5 mm. In addition, the catalyst-substituted zeolite catalyst 100 is in a semi-solid state, and a certain amount of the liquid solvent 50 can be mixed for wet processing.

In this case, the mixing can be performed in the storage tank 210, and the stirring tank is installed in the storage tank 210 so that the catalyst-substituted zeolite catalyst 100 is mixed with the crushing material 60 without being hardened .

Thereafter, the catalyst-substituted zeolite catalyst 100 mixed with the crushing material 60 is moved to the processing tank 220 and subjected to nano-processing and atomization. In this step, the crushing material 60 is agitated by the agitator 222 in the catalyst-substituted zeolite catalyst 100. Then, the crushing material 60 crushes the particles constituting the catalyst-displacing type zeolite catalyst 100 in nanoseconds. In this case, it is preferable that the pressure is important factor in the crushing, so that the pressure gauge 224 is installed in the processing tank 220.

The atomized catalyst-exchanged zeolite catalyst 100 is moved to the back pressure regulating tank 230 to regulate the pressure flowing into the processing tank 220 and the pressure discharged from the processing tank 220. In this case, the back pressure regulating tank 230 is provided with a motor 232 to regulate the back pressure.

A nanofilter 226 may be disposed between the back pressure control tank 230 and the processing tank 220. The nanofilter 226 continues to be atomized in the processing tank 220 without passing particles having a size of 600 nm or more among the substances passing through the nanofilter 226.

The processing step may be performed in a single cycle. However, by continuously transferring the catalyst-substituted zeolite catalyst 100 to the storage tank 210, the processing tank 220, and the back-pressure regulating tank 230, and repeating several tens of cycles or several hundreds of cycles, Substituted zeolites can be prepared.

When the catalyst-substituted zeolite catalyst (100) in which the metal catalyst (120) is substituted is atomized in nano unit, the antimicrobial, antiviral, antifungal and deodorizing effect is excellent and the duration of the effect is unexpectedly long . In this case, when the particle size of the liquid catalyst-substituted zeolite is in the range of 3 nm to 600 nm, the antimicrobial effect is excellent and the duration thereof is also prolonged.

Subsequently, the liquid-phase catalyst-substituted zeolite is diluted in an aqueous solvent and stirred to obtain a uniform state to complete a preservation solution of the bio-cellulose sheet.

In this step, the aqueous solvent may be pure water or distilled water, or water or distilled water having a pH of 7 to 8 may be used.

The above-described preserving solution for a bio-cellulose sheet can be sprayed and can be easily sprayed on a bio-cellulose sheet to achieve desired effects such as enhancing storability or improving repelling force. Unlike conventional preservatives, these preservatives are very harmless to the human body and have excellent preservative performance.

A method of manufacturing a bio-cellulose mask pack using the preserving solution for a bio-cellulose sheet of the present invention will now be described.

The preserving solution for the bio-cellulose sheet can be prepared by adding a catalyst-substituted zeolite (liquid-phase catalyst-substituted zeolite) to water and stirring the same to obtain a homogeneous mixture and stirring the same.

The content of the catalyst-substituted zeolite in the preservative for the bio-cellulose sheet is 0.1 to 10% by weight, for example, 0.1 to 1.0% by weight, for example, 0.2 to 0.5% by weight, based on the total weight of the preservative liquid. The particle size of the catalyst-substituted zeolite in the above-mentioned preserving liquid is preferably 3 nm to 600 nm.

The cultured bio-cellulose may be, for example, in the form of a jellied food. The hexahedron bio-cellulose is sliced to a thickness of the mask pack and laminated.

A step of containing zeolite in the sliced bio-cellulosic sheet. This is because the process continues as a wet bio-cellulose sheet in which the bio-cellulose sheet is wet. In the case of wet bio-cellulose, it has an advantage of being excellent in smoothness and having an excellent adhesion between sheets, and thus being easy to laminate.

However, when wet bio-cellulose is applied, there arises a problem that bio-cellulose as a natural material is corroded. Conventionally, a wet bio-cellulose sheet can not be applied due to the corrosion problem.

However, the present invention allows preservatives to function with a zeolite that is not harmful to the human body.

First, the wet bio-cellulosic sheet is washed, squeezed and drained. If the wet bio-cellulose is made into a sheet, the surface may be contaminated.

At the time of the washing, the preserving liquid is firstly added to the bio-cellulose sheet.

Then, in the step of pressing the bio-cellulose sheet by a press or the like to remove the water, the catalytic-displacement-type zeolite contained in the preserving liquid is contained in the bio-cellulose sheet primarily. It is possible to prevent the bio-cellulose sheet from being corroded for a short period of time. At this time, the bio-cellulose sheet can be laminated.

The bio-cellulose sheet can then be subjected to a boiling process.

Thereafter, the laminated bio-cellulose sheet is subjected to a step of trimming the shape of the bio-cellulose sheet according to the shape of a person.

Thereafter, the above-mentioned preserving liquid is sprayed on the top of the bio-cellulose sheet to impregnate the biocellulose sheet to prepare a second-preserved bio-cellulosic sheet.

Thereafter, the stacked bio-cellulose sheet is put into a wrapping paper.

Thereafter, the inside of the wrapping paper is vacuum-squeezed to discharge the water to the outside. The catalyst-substituted zeolite is uniformly contained in the bio-cellulose sheet through the vacuum compression process.

The bio-cellulosic sheet packed with the preserved bio-cellulosic sheet according to the above-described process is manufactured according to the following procedure.

A catalyst-substituted zeolite is added to water and stirred to obtain a catalyst-substituted zeolite-containing mixture in a homogeneous state. The content of the catalyst-substituted zeolite is from 0.1 to 10% by weight, for example from 0.1 to 1.0% by weight, for example from 0.2 to 0.5% by weight, based on the total weight of the cosmetic mixture. The water content may have a content range of the components other than the components (catalyst-substituted zeolite, thickener and essence) constituting the cosmetic mixture solution.

A solvent such as alcohol may be added in addition to water. The total content of water or water and the solvent is, for example, 74.9 to 99.88% by weight, based on the total weight of the cosmetic mixture.

According to one embodiment, the content of water is adjusted to be in the range of 500 to 5000 parts by weight with respect to 1 part by weight of the catalyst-substituted zeolite. The particle size of the catalyst-substituted zeolite is preferably 3 nm to 600 nm.

The catalyst-substituted zeolite may be prepared by the steps of: preparing a catalyst-substituted zeolite catalyst by replacing a metal-substituted catalyst with zeolite powder; Replacing the catalyst-displacing zeolite catalyst by nanofiber processing to produce a liquid catalyst-substituted zeolite having a catalyst-substituted zeolite having a particle size of 1 nm to 990 nm; And diluting the catalyst-substituted zeolite with water to prepare a nanocatalyst-substituted zeolite aqueous solution.

An aging agent is added to the catalyst-substituted zeolite-containing mixture.

The thickening agent may be a water-soluble thickener such as starch, xanthan gum, dextran, guar gum, locust gum gum, gum arabic, gellan gum, karaya gum, tara gum, tragacantha gum, cellulose gum, acacia gum, pectin, alginate , Carrageenan, agar, and combinations thereof. The content of the thickener is from 0.01 to 5% by weight, for example from 0.05 to 2% by weight, for example from 0.1 to 1% by weight, based on the total weight of the cosmetic mixture. The thickener helps to maintain uniformly dispersed components of cosmetic mixture and control viscosity to an appropriate range.

Then, the essence is added to the mixture to prepare an essence-containing mixed liquid. The thus-obtained essence-containing mixed solution is filled in the preserved bio-cellulose sheet obtained according to the above-mentioned process, and is sealed to prepare a desired bio-cellulose mask pack.

The essence may contain various cosmetic ingredients such as moisturizing, whitening, and nutritional supply effects, and may contain nutritive substances and functional substances such as vitamins and natural extracts. Functional ingredients include ingredients that help skin whitening (arbutin, niacinamide, ascoglucoside), ingredients that help skin wrinkles (retinol, adenosine), sunscreen-aiding ingredients (titanium dioxide) A mixture of one or two or more of skin and hair conditioning components, an antibacterial component, and / or a mixture of one or more selected from water-soluble collagen, vegetable platinum, tocopherol, xylitol and vegetable extracts.

The content of the essence is not particularly limited, but is in the range of 0.01 to 10% by weight based on the total weight of the cosmetic mixture.

In the method of manufacturing a mask pack of the present invention, a catalyst-displacing type zeolite is added to and stirred in water as described above, and then a thickener and an essence are added thereto. This process sequence is very important, and it is very important that if other process orders (e.g., catalyst-substituted zeolites, water, thickener and essence are mixed and stirred simultaneously or the agitator and essence are premixed first, , The catalyst-substituted zeolite can not be uniformly dispersed in the cosmetic mixture, and it becomes very difficult to obtain a mask pack having an excellent preservative effect.

The mask pack is prevented from being spoiled by bacteria and yeast. The bacterium and yeast are selected from the group consisting of Eacherichia coli, Pseudomonas aeruginosa and Staphlyococcus aureus, Candida Is selected from the group consisting of Candida albicans, Aspergillus niger, Bacillus subtilis, Bacillus megaterium and Exophiala moniliae.

In particular, when the mask pack of the present invention is used, it can be used not only for bacteria but also for preventing corruption caused by yeast, and exhibits excellent preservative effect in both bacteria and yeast.

The bio-cellulosic mask pack of the present invention does not propagate microorganisms, has excellent adhesion, and has little skin irritation. And it has excellent cooling effect and moisturizing effect by bio-cellulose, it can supply nutrients to skin and maintain elasticity.

Will be explained in more detail through the following examples and comparative examples. However, the embodiments are for illustrative purposes only and are not intended to be limiting.

Preparation Example 1: Preparation of zeolite

First, the zinc zeolite catalyst 100 was mixed with tritium (size: about 0.2 mm) as a crushing material in the reservoir of FIG. 1, mixed with water and stirred.

Subsequently, the catalyst-substituted zeolite catalyst mixed with the crushing material was moved to the processing tank 220 and agitated by the agitator 222 to atomize it. After the agitation process, the crushed material causes the particles of the catalyst-substituted zeolite catalyst 100 to be crushed in a nanometer scale.

The atomized catalyst-substituted zeolite catalyst 100 is moved to the back pressure regulating tank 230 to regulate the pressure of the gas entering the processing tank 220 and the pressure exiting the processing tank 220.

A nanofilter 226 is disposed between the back pressure regulating tank 230 and the processing tank 220 so that the particles having a size of 600 nm or more in the material passing through the nanofilter 226 are not passed but are atomized in the processing tank 220 .

The catalytic replacement type zeolite catalyst 100 is continuously transferred to the storage tank 210, the processing tank 220 and the back pressure regulating tank 230 to repeat the cycle to obtain a liquid phase having a size of about 200 nm, Zinc zeolite. The content of water in the liquid zinc zeolite is about 500 parts by weight based on 1 part by weight of the zinc zeolite

Preparation Example 2: Preparation of a bio-cellulose sheet to which a preservative was added

1000 mL of the aged coconut solution was heated to 100 DEG C for 30 minutes, and then 100 g of sucrose and 10 g of ammonium sulfate were added and adjusted to pH 4.5 with acetic acid and stirred to prepare a coconut culture. Acetobacter xylenol was inoculated into 100 ml of the prepared culture solution by pre-culture, followed by shaking culture at 30 ° C for 3 days to prepare a culture strain.

5 mL of the pre-cultured strain was injected into the prepared culture, followed by culturing at 30 DEG C for 15 days to prepare a 10-mm-thick bio-cellulose.

After compressing flat plate type bio-cellulose,

Thereby completing the mask pack sheet.

The completed mask pack sheet is sprayed with a preserving liquid to impregnate the mask pack sheet

This was sealed with vinyl to prepare a bio-cellulose sheet to which a preservative was added.

The preservation solution was prepared according to the following procedure.

To the water was added the liquid zinc zeolite obtained according to Production Example 1 and stirred to obtain a uniform mixture to obtain a preservative liquid. The content of zinc zeolite in the liquid zinc zeolite in the preserving solution is 0.4% by weight, and the remaining amount of water is 99.6% by weight.

Example 1: Manufacture of mask pack sheet using bio-cellulose with preservative solution added

To the water was added the liquid zinc zeolite obtained according to Preparation Example 1 and stirred to obtain a homogeneous mixture. Then, xanthan gum, which is a thickening agent, was added to the mixture. Next, the mixture was added and mixed with the essence to prepare a cosmetic mixed emulsion. The content of the viscose mixed emulsion was controlled to be about 22 to 30 g with respect to one sheet of the bio-cellulose mask pack, which was about 26 g in this example.

The content of the zeolite in the liquid zeolite of the viscous mixed emulsion is 0.05 wt%, and the content of the thickener is 0.5 wt%. The content of the essence is controlled so that the essence of 22 to 30 g per one mask pack sheet is controlled. In this embodiment, about 26 g of one mask pack sheet is contained. The viscous liquid was prepared by mixing 100 g of purified water, 0.057 g of zeolite, 5.0 g of glycerin, 4.0 g of betaglucan, 2.5 g of betaine, 0.65 g of caprylic / capric triglyceride, 0.6 g of butylene glycol, 0.5 g of panthenol, g, and carbomer 0.18g.

The bio-cellulose sheet added to the preservative solution was filled in the viscous mixed fluid according to Example 1 for 72 hours to prepare a desired biocellulose mask pack sheet.

Comparative Example 1

Except that a zinc zeolite was added to water and an aqueous solution containing 0.5% by weight of 3-hydroxybenzoic acid was used in purified water instead of the homogeneous mixture obtained by stirring the same, to obtain a biocellulose mask pack Sheet.

Comparative Example 2

A process of irradiating the bio-cellulose sheet with a gamma ray was performed to preserve the bio-cellulose sheet.

When the process of preserving the bio-cellulose sheet according to the comparative example 2 is carried out, unpleasant odor is produced in the bio-cellulose sheet as compared with the process of preserving the bio-cellulose sheet according to the production example 2, I could confirm.

Evaluation Example 1: Evaluation of retention

The biocellulose sheet to which the preservative was added according to Preparation Example 2 and the bio-cellulose sheet which had been preserved according to Comparative Example 2 were evaluated after 20 hours passed and the degree of discoloration and the degree of sheet odor were evaluated to evaluate the retention of each sheet. Respectively.

	smell	Degree of discoloration
Production Example 2	Almost no smell	Little discoloration
Comparative Example 2	There is an unpleasant smell	Yellowish discoloration

Referring to Table 1, it was found that the bio-cellulose sheet to which the preservative solution was added according to Preparation Example 2 had better retention than the bio-cellulose sheet that was preserved according to Comparative Example 2. Evaluation Example 2:

(1) Confirming buoyancy against bacteria

In the mask pack of Example 1, each of the mask packs 20 to 30g was filled with each of Ericia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027), and Pseudomonas aeruginosa Or Staphlyococcus aureus (ATCC 6538) were added to an initial concentration of 10 6 cfu (colony forming unit) / g per g of sample.

These mask packs containing the mycorrhizae were cultured in a thermostat of 30 to 32 for 4 weeks, and 1 g of each cosmetic was taken at intervals of 2, 7, 14, 21 and 28 days, and viable cell counts were measured. And Figs. 5A to 5C, Figs. 6A to 6C and Figs. 7A to 7C. In order to examine the contamination prevention effect of the mask pack sheet completed according to the first embodiment, a mask pack sheet not subjected to a preservation treatment was used as a control group, and the mask pack sheet of the present invention was used as an experimental group.

FIGS. 5A to 5C are photographs showing the result of a test for confirming the flotation resistance against the bactericide of Escherichia coli (ATCC 8739) of a mask pack manufactured according to Example 1. FIGS. 6A to 6C are photographs (Pseudomonas aeruginosa; ATCC 9027) of the mask pack prepared according to the method of the present invention. 7A to 7C are photographs showing the result of a test for confirming the flushing force against the bactericide of Staphlyococcus aureus (ATCC 6538) of the mask pack manufactured according to Example 1. Fig.

	Inoculation time (day)
	0	2	7	14	21	28
One	2.56X10 7	<10	<10	<10	1.00X10 1	4.00X10 1
2	1.11X10 7	<10	<10	2.00X10 1	<10	1.00X10 1
3	2.50X10 7	1.00X10 1	3.00X10 1	1.00X10 1	<10	2.00X10 1

1: Eacherichia coli (ATCC 8739)

2: Pseudomonas aeruginosa (ATCC 9027),

3: Staphlyococcus aureus (ATCC6538)

With reference to Table 2 and FIGS. 5A to 5C, 6A to 6C, and 7A to 7C, when the mask pack of Example 1 was used, general bacteria were detected in the mask pack sheet of the control group, No general bacteria were detected in the mask pack. That is, the mask pack sheet of the present invention can be used in the process of manufacturing a mask pack, such as eacherichia coli (ATCC 8739), Pseudomonas aeruginosa (ATCC 9027), or Staphlyococcus aureus ATCC6538) was effectively prevented from contamination with the bacterial solution.

(2) Confirming buoyancy against fungi (yeast)

In the mask pack prepared in Example 1, Candida albicans (ATCC 10231) or Aspergillus niger (Aspergillus niger) was added to 20 to 30 g of these mask packs to evaluate the repellency against yeast (mold) niger ATCC 8734) was added to the sample to an initial concentration of 10 6 cfu / g. The mixture was incubated for 4 weeks in a constant temperature bath at 25 ° C for 1 week at 2, 7, 14, 21, And the viable cell count was measured. The results are shown in the following Table 3, FIG. 3A to FIG. 3C and FIGS. 4A to 4C. In order to examine the contamination prevention effect of the mask pack sheet completed according to the first embodiment, a mask pack sheet not subjected to a preservation treatment was used as a control group, and the mask pack sheet of the present invention was used as an experimental group.

FIGS. 3A to 3C are optical microscope photographs showing the result of a test for confirming the flotation force against Aspergillus niger ATCC 16404 of a mask pack prepared according to Example 1. FIG.

4A to 4C are optical microscope photographs showing the result of a test for confirming the flushing force against Candida albicans (ATCC 10231) in the mask pack prepared in Example 1. FIG.

	Inoculation time (day)
	0	2	7	14	21	28
4	3.20X10 5	<10	<10	<10	<10	<10
5	1.21X10 6	2.00X10 1	<10	<10	<10	<10

4: Aspergillus niger ATCC 16404 5: Candida albicans (ATCC 10231)

3, 4A, 4B and 4C, almost no fungus was detected as a result of using the mask pack of Example 1, and thus Candida albicans (ATCC 10231) or Aspergillus niger ATCC 8734) was found to be excellent against bactericides.

Evaluation Example 3: Skin irritation test

The mask pack prepared according to the above Example 1 and Comparative Example 1 was applied to the lower limbs of the male and female experimenists (9 female and 6 male) according to the closure pellet test method, and after the plastic cap was covered, The degree of stimulation was observed. The criteria for the degree of stimulation are as follows (see FIG. 8). The evaluator's visual evaluation was conducted by Frosch & Kligman and The Cosmetic, Toiletry, a Fragrance

(CTRA) Guideline (see FIG. 8).

0: No stimulation 1: Mild stimulation

2: erythema, 3: severe irritation such as erythema and edema

4: Severe irritation such as erythema and edema

The degree of stimulation of each subject was measured and divided by the number of subjects, and the average degree of stimulation was calculated. Table 3 below is an average of the degree of stimulation calculated by the above determination method. The results of skin irritation test of the subject are shown in FIGS. 9 and 10. FIG.

division	medium
Example 1	0
Comparative Example 1	2.5

As can be seen from the results of Table 4 and FIG. 9-10, it can be seen that the mask pack manufactured according to Embodiment 1 has little skin irritation. In the foregoing, one embodiment has been described with reference to the drawings and embodiments It will be understood by those skilled in the art that various changes and modifications may be made without departing from the scope of the present invention. Accordingly, the scope of protection of the present invention should be determined by the appended claims.

The present invention is applicable to a mask pack.

Claims (6)

A catalyst-substituted zeolite having a size of 1 to 990 nm, and an aqueous solvent.

The method according to claim 1,

In the catalyst-substituted zeolite,

Replacing the metal-substituted catalyst with a zeolite powder to prepare a catalyst-substituted zeolite catalyst;

Replacing the catalyst-displacing zeolite catalyst by nanofiber processing to produce a liquid catalyst-substituted zeolite having a catalyst-substituted zeolite having a particle size of 1 nm to 990 nm; And

And a step of diluting the catalyst-substituted zeolite with water to prepare an aqueous solution of a nano-catalyst-substituted zeolite, wherein the product is a preservative for a biocellulosic sheet.

The method according to claim 1,

The catalyst-substituted zeolite may be at least one selected from the group consisting of Ag, Cu, Fe, Co, Mn, Zn, Ti, Mg, , Palladium (Pd), zirconium (Zr), and rhodium (Rh)

The content of the catalyst-substituted zeolite is 0.1 to 10% by weight based on the total weight of the preservative.

The method according to claim 1,

A first step of adding a catalyst-substituted zeolite to water and stirring the resultant to obtain a catalyst-substituted zeolite aqueous solution containing a catalyst-substituted zeolite having a particle size of 1 nm to 990 nm;

A second step of preparing a preservative solution for a bio-cellulose sheet using the catalyst-substituted zeolite aqueous solution; And

And a third step of filling the bio-cellulose sheet with the preserving solution for the bio-cellulose sheet.

5. The method of claim 4,

The catalyst-displacing zeolite aqueous solution of the first step may contain,

Replacing the metal-substituted catalyst with a zeolite powder to prepare a catalyst-substituted zeolite catalyst;

Replacing the catalyst-displacing zeolite catalyst by nanofiber processing to produce a liquid catalyst-substituted zeolite having a catalyst-substituted zeolite having a particle size of 1 nm to 990 nm; And

And diluting the catalyst-substituted zeolite with water to prepare a nanocatalyst-substituted zeolite aqueous solution.

5. The method of claim 4,

The catalyst-substituted zeolite may be at least one selected from the group consisting of Ag, Cu, Fe, Co, Mn, Zn, Ti, Mg, , Palladium (Pd), zirconium (Zr) and rhodium (Rh).

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