WO2018216709A1 - 殺菌方法、殺菌装置 - Google Patents

殺菌方法、殺菌装置 Download PDF

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Publication number
WO2018216709A1
WO2018216709A1 PCT/JP2018/019749 JP2018019749W WO2018216709A1 WO 2018216709 A1 WO2018216709 A1 WO 2018216709A1 JP 2018019749 W JP2018019749 W JP 2018019749W WO 2018216709 A1 WO2018216709 A1 WO 2018216709A1
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Prior art keywords
ultraviolet light
light
solution
treated
sterilization
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Ceased
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English (en)
French (fr)
Japanese (ja)
Inventor
敬祐 内藤
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Ushio Denki KK
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Ushio Denki KK
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Priority to KR1020197034259A priority Critical patent/KR102375198B1/ko
Priority to EP18805141.1A priority patent/EP3632216A4/en
Priority to CN201880034614.8A priority patent/CN110678084A/zh
Priority to US16/616,510 priority patent/US20200178573A1/en
Publication of WO2018216709A1 publication Critical patent/WO2018216709A1/ja
Anticipated expiration legal-status Critical
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B70/00—Preservation of non-alcoholic beverages
    • A23B70/50—Preservation of non-alcoholic beverages by irradiation or electric treatment, without heating
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B2/00—Preservation of foods or foodstuffs, in general
    • A23B2/50—Preservation of foods or foodstuffs, in general by irradiation without heating
    • A23B2/53—Preservation of foods or foodstuffs, in general by irradiation without heating with ultraviolet light
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23B—PRESERVATION OF FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES; CHEMICAL RIPENING OF FRUIT OR VEGETABLES
    • A23B70/00—Preservation of non-alcoholic beverages
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F5/00—Coffee; Coffee substitutes; Preparations thereof
    • A23F5/24—Extraction of coffee; Coffee extracts; Making instant coffee
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof containing fruit or vegetable juices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12H—PASTEURISATION, STERILISATION, PRESERVATION, PURIFICATION, CLARIFICATION OR AGEING OF ALCOHOLIC BEVERAGES; METHODS FOR ALTERING THE ALCOHOL CONTENT OF FERMENTED SOLUTIONS OR ALCOHOLIC BEVERAGES
    • C12H1/00—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages
    • C12H1/12—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation
    • C12H1/16—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation by physical means, e.g. irradiation
    • C12H1/165—Pasteurisation, sterilisation, preservation, purification, clarification, or ageing of alcoholic beverages without precipitation by physical means, e.g. irradiation by irradiation
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the present invention relates to a sterilization method and a sterilization apparatus for beverages other than water.
  • UV sterilization is considered as a method that is less likely to deteriorate the flavor than heat sterilization.
  • a mercury lamp the main emission wavelength is 254 nm
  • UV sterilization is considered as a method that is less likely to deteriorate the flavor than heat sterilization.
  • a mercury lamp the main emission wavelength is 254 nm
  • Patent Document 3 a device that irradiates ultraviolet light as a sterilization treatment for fruit juice, jelly, mousse and the like which are food and drink is described.
  • This document describes that sterilization is performed by using ultraviolet light having a wavelength range of 200 to 300 nm, particularly 220 nm to 280 nm (UV-C).
  • JP 2004-201535 A Japanese Patent No. 5924394 International Publication No. 2016/186068
  • This invention aims at providing the sterilization method and apparatus which suppressed the deterioration of flavor, ensuring the sterilization effect in the sterilization method with respect to a liquid substance.
  • an ultraviolet light having an emission wavelength of 280 nm or more and 320 nm or less is applied to a solution to be treated, which is a beverage other than water, without substantially irradiating ultraviolet light with an emission wavelength of 260 nm or less. Irradiating.
  • UV-B ultraviolet-B
  • the generation of substances that are the source of bitterness, miscellaneous components and malodorous components that occur when light with a wavelength of 254 nm is irradiated is suppressed. It was confirmed that sterilization was possible while preventing the inhibition of flavor.
  • the sterilization apparatus substantially contains components whose main emission wavelength is 280 nm or more and 320 nm or less and whose emission wavelength is 260 nm or less along the flow path through which the solution to be treated which is a beverage other than water flows.
  • An ultraviolet light irradiation device for irradiating ultraviolet light not included in the above is provided.
  • FIG. 2 is a cross-sectional view taken along line AA in FIG. It is an emission spectrum of each light source of Example 1, Example 2, and Comparative Example 1. It is an analysis result of the smell sniffing GC / GCMS system for coffee beverages. It is the graph which changed and illustrated the drawing mode of the graph of Drawing 4A. It is an analysis result of the smell sniffing GC / GCMS system for apple juice. It is the graph which changed and illustrated the drawing mode of the graph of Drawing 5A. It is an analysis result of the smell smell GC / GCMS system with respect to lemon juice. It is the graph which changed and illustrated the drawing mode of the graph of Drawing 6A.
  • the treatment target in the sterilization apparatus and sterilization method of the present invention is a solution to be treated which is a beverage other than water.
  • a solution to be treated examples include wine, sake, beer and other alcoholic beverages, coffee, juice, and liquid perfume itself.
  • FIG. 1 is a cross-sectional view along the flow path schematically showing the structure of the main part of an example of the sterilization apparatus of the present invention.
  • 2 is a cross-sectional view taken along line AA in FIG. In each drawing, the dimensional ratio on the drawing does not always match the actual dimensional ratio.
  • This sterilizer is provided with a reactor 10.
  • the reactor 10 includes a flow path 3 through which the solution to be treated 2 flows, and an ultraviolet light irradiation device 20 provided along the flow path 3.
  • the ultraviolet light irradiation device 20 is configured to emit ultraviolet light that has a main emission wavelength of 280 nm to 320 nm and substantially does not contain a component having an emission wavelength of 260 nm or less.
  • the light whose main emission wavelength is 280 nm or more and 320 nm or less refers to light whose wavelength band showing the half value of the emission spectrum is in the range of 280 nm or more and 320 nm or less.
  • the light that does not substantially include a component having an emission wavelength of 260 nm or less refers to light that does not include a component of 260 nm or less in a wavelength band that shows a half value of an emission spectrum.
  • the reactor 10 has a cylindrical outer tube 11 and a cylindrical inner tube 12 arranged coaxially with each other.
  • the flow path 3 of the solution to be treated 2 is configured by a region sandwiched between the inner peripheral surface of the outer tube 11 and the outer peripheral surface of the inner tube 12.
  • pipe 11 in the reactor 10 may be any material as long as it transmits ultraviolet light from the ultraviolet light irradiation device 20, and for example, quartz glass can be used.
  • the ultraviolet light irradiation device 20 is disposed along the central axis 5 of the reactor 10 inside the inner tube 12 of the reactor 10.
  • the ultraviolet light irradiation device 20 includes a light source that emits ultraviolet light that has a main emission wavelength of 280 nm to 320 nm and substantially does not include a component of the emission wavelength of 260 nm or less.
  • light for example, light from a low-pressure mercury lamp (254 nm emission line)
  • a component having an emission wavelength of 260 nm or less is emitted to the solution to be treated 2
  • the flavor of the solution to be treated 2 is deteriorated. This point will be described later with reference to examples.
  • an XeBr excimer lamp (a peak wavelength is 283 nm) in which a mixed gas of Xe and Br is sealed as a discharge gas, and an excimer in which Br 2 is sealed as a discharge gas are used.
  • a lamp peak wavelength is 289 nm
  • a XeCl excimer lamp peak wavelength is 308 nm in which a mixed gas of Xe and Cl is sealed as a discharge gas, and the like can be used.
  • an ultraviolet light emitting fluorescent lamp can be used as a light source used as the ultraviolet light irradiation device 20.
  • Ultraviolet radiation fluorescent lamps irradiate phosphors as excitation light with light emitted from excimers generated by dielectric barrier discharge, and emit ultraviolet light in a specific wavelength range obtained when the phosphors are excited. Radiates as light.
  • the phosphor for example, bismuth-activated yttrium aluminum borate that emits ultraviolet light having a peak wavelength of 290 nm by excitation can be used.
  • cerium-activated lanthanum phosphate having a broad emission peak at a peak wavelength near 320 nm by excitation can be used.
  • an LED element configured to have a light emission wavelength of 280 nm or more and 320 nm or less and substantially not including a component having a light emission wavelength of 260 nm or less can be used. .
  • the amount of ultraviolet light applied to the solution to be treated 2 by the ultraviolet light irradiation apparatus 20 is preferably, for example, 170 mJ / cm 2 or more, and more preferably 170 to 500 mJ / cm 2 .
  • the ultraviolet light irradiation amount is preferably, for example, 170 mJ / cm 2 or more, sterilization treatment can be performed while suppressing changes in the taste and odor of the solution to be treated 2 itself.
  • the diameter of the flow path 3 in the reactor 10, that is, the distance between the inner peripheral surface of the outer tube 11 and the outer peripheral surface of the inner tube 12, is preferably 0.05 to 1 mm, for example.
  • the flow rate of the solution 2 to be circulated in the flow path 3 and the size of the region irradiated with ultraviolet light in the flow path 3 (that is, the direction of the flow path 3 of the light source included in the ultraviolet light irradiation device 20)
  • the length) and other conditions can be appropriately set so that the amount of ultraviolet light irradiation is within the specific range.
  • the solution 2 to be treated is introduced into the flow path 3 and radiated from the ultraviolet light irradiation apparatus 20 in the process of flowing through the flow path 3.
  • the to-be-processed solution 2 is sterilized.
  • the reactor 10 only needs to have a structure in which the ultraviolet light irradiation device 20 is provided along the flow path through which the solution to be treated 2 flows, and is not limited to the above structure.
  • a reactor 10 having the following specifications was manufactured according to the configuration shown in FIG.
  • the outer tube 11 is made of stainless steel and has an inner diameter of ⁇ 27 mm.
  • the inner tube 12 is made of quartz glass and has an outer diameter of ⁇ 26.5 mm and a wall thickness of 1.0 mm.
  • the length of the region irradiated with ultraviolet light from the ultraviolet light irradiation device 20 is 80 mm.
  • the distance between the inner peripheral surface of the outer tube 11 and the outer peripheral surface of the inner tube 12, that is, the radial width of the flow path 3 is 0.5 mm.
  • Example 1 As a light source included in the ultraviolet light irradiation device 20, a XeBr excimer lamp that emits ultraviolet light having a peak wavelength of 283 nm (half value: 280 nm to 286 nm) was used. The emission length of the XeBr excimer lamp is 80 mm.
  • Example 2 As a light source included in the ultraviolet light irradiation apparatus 20, an ultraviolet excimer fluorescent lamp (UV-XEFL320BB manufactured by USHIO INC.) That emits ultraviolet light having a peak wavelength of 320 nm (half value: 310 nm to 360 nm) was used. The emission length of the ultraviolet excimer fluorescent lamp is 80 mm.
  • Example 3 As a light source included in the ultraviolet light irradiation apparatus 20, an ultraviolet excimer fluorescent lamp (UV-XEFL290BB manufactured by Ushio Electric Co., Ltd.) that emits ultraviolet light having a peak wavelength of 290 nm (half value: 270 nm to 320 nm) was used.
  • the emission length of the ultraviolet excimer fluorescent lamp is 80 mm.
  • (Comparative Example 1) As a light source included in the ultraviolet light irradiation device 20, a low-pressure mercury lamp that emits ultraviolet light having a peak wavelength of 254 nm (half value is 251 to 257 nm) was used. The light emission length of the low-pressure mercury lamp is 80 mm.
  • (Comparative Example 2) As a light source included in the ultraviolet light irradiation apparatus 20, a KrCl excimer lamp that emits ultraviolet light having a peak wavelength of 222 nm (half value is 215 to 229 nm) was used. The emission length of the KrCl excimer lamp is 80 mm.
  • FIG. 3 shows emission spectra of the light sources of Examples 1 to 3 and Comparative Examples 1 and 2.
  • the irradiation conditions of ultraviolet light are as follows. Flow rate of test solution in treated solution flow path: 0.72 ml / hour Ultraviolet light intensity in treated solution flow path: 3.6 mW / cm 2 Processing time: 139 seconds Ultraviolet light irradiation amount: 500 mJ / cm 2 Solution temperature during treatment: 20 ° C
  • the aroma analysis test was conducted by the following method. About each to-be-treated solution 2 after the ultraviolet light irradiation treatment, an odor component analysis test was performed using a smell sniffing GC / GCMS system (Gas Chromatograph Mass Spectrometer QP-2010Plus manufactured by Shimadzu Corporation). More specifically, an analytical test was performed on each solution to be treated 2 after irradiation with ultraviolet light using the light source of Example 1 and the light source of Comparative Example 1. The results are shown in FIGS. 4A, 4B, 5A, 5B, 6A, 6B, 7A, and 7B. In each graph of FIGS. 4A to 7B, the horizontal axis is the holding time, and the vertical axis is the intensity.
  • FIG. 4B is a graph obtained by redrawing the graphs of the unprocessed, Example 1, and Comparative Example 1 by translating them in the vertical axis direction from the viewpoint of making FIG. 4A easier to see.
  • FIGS. 4A and 4B in Comparative Example 1, 3-methyl-1-butanol that was not present in the untreated and in Example 1 was detected. Since 3-methyl-1-butanol is a substance having an unpleasant odor, it is confirmed that the odor changes from the state before the treatment by performing the treatment with the light source of Comparative Example 1.
  • FIG. 5B is a graph obtained by redrawing the unprocessed graphs of Example 1 and Comparative Example 1 by translating them in the vertical axis direction from the viewpoint of making FIG. 5A easier to see.
  • Cycloheptanone is a substance having a mint odor, and it is confirmed that this odor component increases by processing with the light source of Comparative Example 1 and changes to an odor different from that inherent in apple juice.
  • FIG. 6B is a graph obtained by redrawing the graphs of the unprocessed, Example 1, and Comparative Example 1 by translating them in the vertical axis direction from the viewpoint of making FIG. 6A easier to see. According to the results of FIGS. 6A and 6B, it was confirmed that the strength of ethanol greatly increased in Comparative Example 1. Since ethanol is a substance having an alcoholic odor, it is confirmed that the odor component is increased by processing with the light source of Comparative Example 1, and the odor component is changed to a different odor from the lemon juice.
  • the taste analysis test was conducted by the following method. Each solution to be treated 2 was subjected to ultraviolet light irradiation treatment (sterilization treatment) using the reactor 10 described above. Then, for each solution 2 to be treated after the ultraviolet light irradiation treatment, a taste analysis test was performed using a taste sensor (taste recognition device “TS-5000Z” manufactured by Intelligent Sensor Technology Co., Ltd.). The result is shown in FIG.
  • the chart shown in FIG. 8 shows relative values when the numerical value of each item obtained by analyzing the original taste of the solution to be treated 2 when the ultraviolet light irradiation treatment is not performed is 0.0.
  • a threshold value at which a sensitive person can detect a difference in taste is about ⁇ 0.8.
  • the eight analysis items displayed in the chart of FIG. 8 are as follows.
  • test solution prepared by suspending a predetermined amount of the test bacterium in the solution to be treated 2 so that the initial bacterial count is 10 5 CFU / mL is used as a test bacterium in a spore state of Bacillus cereus (JCM2152).
  • JCM2152 Bacillus cereus
  • a coffee jelly fragrance was adopted as the solution to be treated 2.
  • the number of colonies generated on the agar medium was examined. The result is shown in FIG. According to FIG. 9, it was confirmed that even when the light source of Example 1 was used, the bactericidal effect was secured. In addition, even if it was a case where the light source of Example 2 and Example 3 was used by setting processing time appropriately, it was confirmed that the bactericidal effect can be ensured.
  • the processing time when using the light source of Example 1 is 55 seconds or more and 84 seconds or less, and the processing time when using the light source of Example 2 is 100 seconds or more and 300 seconds or less.
  • This processing time is sufficiently longer than the time of sterilization processing using a flash lamp (msec order) in the field of food such as meat and fish.
  • Meat and fish contain a lot of oil, which is altered by absorbing light. For this reason, in the food field, a method of sterilizing by irradiating light for an extremely short time to instantaneously increase the temperature to be used may be used.
  • the present invention newly discovers that a change in odor and taste occurs by irradiating ultraviolet light in a predetermined wavelength band, and then in a specific wavelength band excluding the predetermined wavelength band. Irradiation with ultraviolet light makes it possible to suppress the change and deterioration of smell and taste while ensuring the bactericidal effect.
  • ultraviolet light having an emission wavelength of 260 nm or less was substantially irradiated. Without irradiating, it is understood that sterilization treatment can be performed while irradiating ultraviolet light having an emission wavelength of 280 nm or more and 320 nm or less while suppressing changes in taste and smell.
  • FIG. 10 is a graph showing the relative value of the bactericidal power for each wavelength when the same amount of light is irradiated.
  • the horizontal axis indicates the wavelength
  • the vertical axis indicates the relative value of the bactericidal power for each wavelength when the bactericidal power at 254 nm is 100%.
  • the bactericidal power of light with a wavelength of 280 nm is about 60% with respect to a wavelength of 254 nm.
  • the sterilizing power of light having a wavelength longer than 320 nm is 0.001% or less with respect to the wavelength of 254 nm.
  • Sterilization ability is improved by increasing the amount of irradiation light. That is, by adjusting the output of the light source and the irradiation time, the sterilizing ability can be substantially secured at a practical level.
  • the effect of sterilization cannot be sufficiently ensured unless the output of the light source is made extremely high and an extremely long irradiation time is ensured.
  • FIG. 1 when it is assumed that sterilization is performed by irradiating light from a light source while flowing the solution to be treated 2 in the flow path 3, a light source that emits light having a wavelength longer than 320 nm is employed. In this case, in order to ensure a very long irradiation time, it is necessary to extremely slow the flow rate of the solution to be treated 2 and to lengthen the length of the light source in the flow path direction, which is not practical.

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  • Engineering & Computer Science (AREA)
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  • Food Science & Technology (AREA)
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PCT/JP2018/019749 2017-05-26 2018-05-23 殺菌方法、殺菌装置 Ceased WO2018216709A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
KR1020197034259A KR102375198B1 (ko) 2017-05-26 2018-05-23 살균 방법, 살균 장치
EP18805141.1A EP3632216A4 (en) 2017-05-26 2018-05-23 STERILIZATION METHOD AND STERILIZATION DEVICE
CN201880034614.8A CN110678084A (zh) 2017-05-26 2018-05-23 杀菌方法、杀菌装置
US16/616,510 US20200178573A1 (en) 2017-05-26 2018-05-23 Sterilization method and sterilization device

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JP2017104646A JP6892788B2 (ja) 2017-05-26 2017-05-26 殺菌方法、殺菌装置
JP2017-104646 2017-05-26

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JP2018198556A (ja) 2018-12-20
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US20200178573A1 (en) 2020-06-11
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