WO2018195682A1 - Dispositif de transfert par buvardage, système de transfert par buvardage et procédé de commande - Google Patents

Dispositif de transfert par buvardage, système de transfert par buvardage et procédé de commande Download PDF

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Publication number
WO2018195682A1
WO2018195682A1 PCT/CN2017/081591 CN2017081591W WO2018195682A1 WO 2018195682 A1 WO2018195682 A1 WO 2018195682A1 CN 2017081591 W CN2017081591 W CN 2017081591W WO 2018195682 A1 WO2018195682 A1 WO 2018195682A1
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Prior art keywords
transfer
biotransfer
electrode layer
signal
electrical signal
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PCT/CN2017/081591
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English (en)
Chinese (zh)
Inventor
吴升海
杜艳芬
侯林
孙亮
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赛默飞世尔（上海）仪器有限公司
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Priority to CN201780085970.8A priority Critical patent/CN110249220B/zh
Priority to PCT/CN2017/081591 priority patent/WO2018195682A1/fr
Publication of WO2018195682A1 publication Critical patent/WO2018195682A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Definitions

  • the invention belongs to the technical field of biochemical analysis and relates to the transfer of biological macromolecules, in particular to a biotransfer device using a unipolar electrical signal with varying fluctuations, a biotransfer system and a control method thereof.
  • a biotransfer device (Blot Transfer Device) is a method for transferring a biomacromolecule (for example, a protein) in a gel layer containing a biomacromolecule onto a carrier film by using an electrophoresis principle. Therefore, the biotransfer device generally includes a control unit. It is used to generate a corresponding electrical signal applied to the gel layer to control the above-described electrophoresis-based transfer process, the electrical signal being applied specifically to the electrodes of the transfer unit comprising the gel layer and the carrier film.
  • a biomacromolecule for example, a protein
  • the biotransfer device generally includes a control unit. It is used to generate a corresponding electrical signal applied to the gel layer to control the above-described electrophoresis-based transfer process, the electrical signal being applied specifically to the electrodes of the transfer unit comprising the gel layer and the carrier film.
  • the molecular size of proteins during transfer is usually inconsistent.
  • the transfer rate under the same electrical signal is different, especially for relative Larger proteins have a relatively low rate of transfer.
  • the transfer efficiency and the transfer quality in the biotransfer device are proportional to the voltage applied to the transfer unit, and therefore, the transfer efficiency is improved and the good is obtained by increasing the voltage applied to the transfer unit.
  • Transfer quality is not necessarily ensured.
  • one of the upper and lower electrode layers of the transfer unit terminal of the protein transfer device is In the multiple composite electrode layer, different electrical signals are controlled to be applied on different electrode layers during the transfer process, and different electrical signals applied on different electrode layers can produce different transfer efficiencies for proteins of different sizes.
  • the overall structure of the solution disclosed in US8721860B2 is complicated, and the control of the transfer process is relatively complicated.
  • One of the objects of the present invention is to effectively avoid the temperature of the transfer unit during transfer Too high
  • Still another object of the present invention is to improve the transfer quality.
  • Still another object of the present invention is to ensure transfer efficiency without substantially increasing the transfer time.
  • the present invention provides the following technical solutions.
  • a biotransfer device comprising a control unit and one or more transfer units, the control unit being configured to: a first plate electrode layer to one or more of the transfer units Transmitting, with the second plate electrode layer, a unipolar electrical signal that periodically fluctuates according to a predetermined amplitude, wherein a ratio of an instantaneous power corresponding to a highest point of the predetermined amplitude to an instantaneous power corresponding to a lowest point of the predetermined amplitude is greater than or Equal to 2.
  • the biotransfer device of the invention is advantageous for maximizing the transfer work efficiency, ensuring the transfer efficiency, reducing the temperature of the transfer unit during the transfer process, reducing heat generation, and improving transfer quality.
  • a biotransfer device wherein the undulating unipolar electrical signal comprises a square wave voltage signal with adjustable duty ratio, or an intermittent sine wave signal, a triangular wave signal, a sawtooth wave signal And at least one of the step signals.
  • the unipolar electrical signals of this embodiment are easy to generate and the associated parameters are easy to control.
  • a biotransfer device wherein the frequency of the unipolar electrical signal is greater than or equal to 1 Hz and less than or equal to 100 Hz, or greater than or equal to 5 Hz and less than or equal to 20 Hz.
  • the frequency range of this embodiment it is more advantageous to avoid the continuous heat generation and temperature rise of the transfer unit, further improving the transfer quality.
  • a biotransfer device wherein a duty ratio of the unipolar electrical signal is from 1% to 99%, or from 30% to 60%.
  • the unipolar electrical signal of this embodiment provides an adjustable duty cycle range.
  • biotransfer device according to an embodiment of the present invention, wherein the biotransfer device further includes temperature information for measuring the first plate electrode layer and/or the second electrode layer in real time during transfer Temperature sensor
  • the control unit is further configured to dynamically adjust the duty cycle based on at least the temperature information during transfer.
  • the biotransfer device of this embodiment can be dynamically based on temperature information feedback
  • the adjustment of the duty ratio makes the adjustment of the unipolar electrical signal more accurate, and is more advantageous for maximizing the efficiency of the transfer work, further reducing the temperature of the gel layer and the carrier film, reducing the heat generation, and improving the transfer quality.
  • control unit is further configured to: adjust at least the duty ratio and/or the maximum instantaneous power to cause the transfer unit to be in a transfer process
  • the temperature is below 60 ° C - 70 ° C. Therefore, it is possible to completely avoid coking of the gel layer or the like of the transfer process transfer unit.
  • a biotransfer device according to an embodiment of the present invention, wherein the transfer unit comprises:
  • the first plate electrode layer and the second plate electrode layer disposed substantially in parallel;
  • first buffer medium layer between the first plate electrode layer and the second plate electrode layer, a gel layer containing a biomacromolecule, a carrier film, and a second buffer medium layer;
  • biomacromolecule in the gel layer is electrophoresed to the carrier film by the applied unipolar electrical signal.
  • a biotransfer device wherein a ratio of a resistance of the first buffer dielectric layer, the second buffer dielectric layer, and the carrier film to a resistance of the gel layer is less than or equal to 3.
  • the arrangement of the transfer unit of the embodiment of the present invention is advantageous for further improving the transfer work efficiency.
  • a biotransfer device wherein energy and/or information transmission is achieved between the control unit and at least one of the transfer units by non-contact electromagnetic coupling. Therefore, the structure of the transfer device of the embodiment of the invention can be designed to be more compact, easier to achieve overall waterproof, biological or chemical pollution, more convenient and flexible to use, and is very suitable for use in a biological laboratory.
  • control unit comprises:
  • control parameter generation module for generating an adjusted control parameter based on the measurement information or/and the transfer quality information
  • a unipolar electrical signal generating module configured to generate the unipolar electrical signal according to the adjusted control parameter
  • the adjusted control parameter includes at least one of a waveform, a frequency, a voltage value, a current value, and a duty ratio of the unipolar electrical signal;
  • the measurement information is temperature information of the first plate electrode layer and/or the second plate electrode layer measured in real time during the transfer process, or is recorded during the transfer process And including at least one of a waveform, a frequency, a voltage value, a current value, and a duty ratio of the unipolar electrical signal, and temperature information of the first plate electrode layer and/or the second plate electrode layer Timing information.
  • the biotransfer device of the embodiment of the present invention is advantageous in further reducing the work for heat radiation and temperature increase during the transfer process, thereby contributing to lowering the temperature of the transfer unit and improving the transfer quality.
  • a biotransfer system comprising:
  • a cloud server coupled to a plurality of control units of the biotransfer device
  • the cloud server is configured to include:
  • a history database for storing measurement information or/and transfer quality information records obtained by each of the plurality of biotransfer devices at each transfer process as historical data information
  • a cloud computing module for calculating a control parameter for generating a current transfer process corresponding to one of the plurality of biotransfer devices based on the historical data information
  • a sending module configured to send the control parameter to a corresponding control unit of the biotransfer device
  • control parameter includes at least one of a waveform, a frequency, a voltage value, a current value, and a duty ratio of the unipolar electrical signal;
  • the measurement information is that the first tablet is measured in real time during a transfer process Temperature information of the electrode layer and/or the second plate electrode layer, or a waveform, a frequency, a voltage value, a current value, and a duty ratio including the unipolar electrical signal recorded during the transfer, and Timing information of at least one of temperature information of the first plate electrode layer and/or the second plate electrode layer.
  • the biotransfer system of the invention can quickly determine the control parameters conforming to the current transfer process based on historical data information, and is easy to implement the self-learning function, greatly improving the user experience, and greatly reducing the professional skills and experience requirements of the user or the experimenter.
  • a control method for a biotransfer device comprising one or more transfer units, the transfer unit comprising a first plate electrode layer and the second a plate electrode layer, and a gel layer and a carrier film between the first plate electrode layer and the second plate electrode layer; wherein the first plate electrode layer and the first to the transfer unit
  • the two plate electrode layers uniformly apply a unipolar electrical signal that periodically fluctuates according to a predetermined amplitude, wherein a ratio of an instantaneous power corresponding to a highest point of the predetermined amplitude to an instantaneous power corresponding to a lowest point of the predetermined amplitude is greater than or Equal to 2.
  • the control method of the biotransfer device of the present invention is advantageous in maximizing the transfer work efficiency, ensuring the transfer efficiency, reducing the temperature of the transfer unit in the transfer process, reducing heat generation, and improving transfer quality.
  • the undulating unipolar electrical signal comprises a square wave voltage signal with adjustable duty ratio, or an intermittent sine wave signal, a triangular wave signal, a sawtooth wave signal, and a ladder At least one of the signals.
  • the unipolar electrical signals of this embodiment are easy to generate and the associated parameters are easy to control.
  • the fluctuation frequency of the unipolar electrical signal is greater than or equal to 1 Hz and less than or equal to 100 Hz, or greater than or equal to 5 Hz and less than or equal to 20 Hz. In the frequency range of this embodiment, it is more advantageous to avoid the continuous heat generation and temperature rise of the transfer unit, further improving the transfer quality.
  • control method further includes:
  • the adjusted control parameter including at least one of a waveform, a frequency, a voltage value, a current value, and a duty ratio of the unipolar electrical signal ;as well as
  • the control method of the biotransfer device of this embodiment can dynamically adjust the duty ratio based on the temperature information feedback, and the adjustment of the unipolar electrical signal is more accurate, which is more advantageous for maximizing the transfer work efficiency and further reducing the condensation.
  • the temperature of the glue layer and the carrier film and the like reduce heat generation and improve transfer quality.
  • Figure 1 is a schematic view showing the structure of a biotransfer device in accordance with a first embodiment of the present invention.
  • Fig. 2 is a schematic view showing the structure of a transfer unit in the biotransfer device of the first embodiment shown in Fig. 1.
  • FIG. 3 is a schematic diagram of a unipolar electrical signal according to an embodiment of the invention, wherein FIG. 3(a) is a square wave voltage signal with adjustable duty ratio, and FIG. 3(b) is a transformation of FIG. 3(a).
  • FIGS. 4(a) and 4(b) are sinusoidal voltage signals
  • FIGS. 4(c) and 4(d) are The triangular voltage signal
  • Fig. 4(e) and Fig. 4(f) are sawtooth voltage signals
  • Fig. 4(g) and Fig. 4(h) are step voltage signals.
  • FIG. 5 is a block diagram showing the functional blocks of a control unit of a biotransfer device in accordance with an embodiment of the present invention.
  • Figure 6 is a graph showing the relationship between the resistance ratio and the transfer effective work.
  • Fig. 7 is a graph showing the calculated transfer effective work distribution.
  • Fig. 8 is a schematic view showing the control principle of the biotransfer device of the embodiment of the present invention.
  • Figure 9 is a schematic view showing the structure of a biotransfer device in accordance with a second embodiment of the present invention.
  • Figure 10 is a schematic view showing the structure of a biotransfer device in accordance with a third embodiment of the present invention.
  • FIG. 11 is a schematic structural view of a non-contact electromagnetically coupled power supply module used in a biotransfer device according to an embodiment of the present invention.
  • Fig. 12 is a schematic structural view of a biotransfer system of a first embodiment formed based on the biotransfer device of the embodiment shown in Fig. 9.
  • Figure 13 is a schematic structural view of a biotransfer system of a second embodiment formed based on the biotransfer device of the embodiment shown in Figure 10.
  • FIG. 14 is a block diagram showing the structure of a cloud server of a biotransfer system according to an embodiment of the present invention.
  • the biotransfer device 10 of the embodiment of the present invention mainly includes a transfer unit 130 and a control unit 11, wherein the transfer unit 130 is a specific execution component of the transfer process, and the main function of the control unit 11 is to control the specificity of the transfer unit 130.
  • the working process is specifically achieved by controlling the electrical signal applied to the transfer unit 130.
  • Fig. 2 is a view showing the structure of a transfer unit in the biotransfer device of the embodiment shown in Fig. 1.
  • the transfer unit 130 is mainly formed of a stacked structure 131 stacked between upper and lower electrodes and upper and lower electrodes, which specifically includes an upper plate electrode layer 1301 and a lower plate electrode layer 1302, and is located on the upper plate electrode layer 1301 and below.
  • the function of the transfer unit 130 is to apply an electric signal to the upper plate electrode layer 1301 and the lower plate electrode layer 1302, thereby generating an electric field E similar to the direction indicated by the dotted arrow in FIG. 2, the biomacromolecules in the gel layer 131d. Under the action of the electric field E, the biomacromolecule is transferred to the carrier film 131c in accordance with the electrophoresis movement in the direction indicated by the time limit arrow in Fig. 2, and the transfer process is completed.
  • the transfer process of the protein will be exemplified, but it should be understood that the biomacromolecules in the gel layer 131d to be transferred are not limited to proteins, and may be other similar Biological macromolecules, such as DNA.
  • the biotransfer device 10 is specifically a Western blotting device; when the biotransfer device 10 is used for transferring DNA, it is specifically a Southern blotting device.
  • the upper plate electrode layer 1301 and the lower plate electrode layer 1302 may be formed of various conductive materials, and the specific material type thereof is not limited.
  • the "plate electrode layer” means a planar single layered electrode structure (for each laminated structure 131 of the transfer unit), and an electric signal is applied to the upper plate electrode layer 1301 and the lower plate electrode.
  • the layer 1302 is on, the same electrical signal is uniformly applied between the upper plate electrode layer 1301 and the lower plate electrode layer 1302, and a uniform electric field E as shown in FIG. 2 is formed between the upper plate electrode layer 1301 and the lower plate electrode layer 1302.
  • the electric field E is uniformly applied to proteins of different sizes in the gel layer 131d, that is, proteins of different sizes act on the uniform electric field E for electrophoresis.
  • Upper plate electrode layer of embodiment of the invention The lower plate electrode layer 1302 has a simple structure and is simple to prepare.
  • the "uniformly" application of the electric field means that the electric field of the electrophoretic movement of the biomolecule of the gel layer 131d is uniform and simultaneously acts with respect to all the biomolecules such as the protein of the gel layer 131d.
  • the upper plate electrode layer 1301 and the lower plate electrode layer 1302 are disposed in a relatively parallel manner, such that the upper plate electrode layer 1301 and the lower plate electrode layer 1302 are not only spatially uniform.
  • a certain electrical signal is applied, and the distribution of the electric field E generated by the electrical signal in the left-right direction as shown in FIG. 2 is also substantially uniform.
  • the upper buffer dielectric layer 131e is located between the gel layer 131d and the upper plate electrode layer 1301, and the lower buffer dielectric layer 131b is located between the carrier film 131c and the lower plate electrode layer 1302. They may be formed with a buffer gel or filter paper material that first has a conductive effect, also has a protective effect on the gel layer 131d and the carrier film 131c, and provides a buffer ion for transfer.
  • the resistance buffer layer 131e and the lower dielectric layer 131b of the cushioning medium can be measured or estimated from the characteristics of the material selected for use, are recorded as R e and R b.
  • the cushioning medium layer resistance R e 131e and / or the lower dielectric buffer layer R b 131b is depending on the material, temperature change and changes in the number of buffer varies.
  • the gel layer 131d can be a biomass membrane that has, for example, a gel having a gradient concentration of 4%-20% or 4%-12%, or at 4%-12 A uniform concentration of gel in the range of %, different sizes of proteins to be transferred may be electrophoresed in the gel layer 131d; the thickness of the gel layer 131d may range from 0.5 mm to 2.0 mm, for example, may be 1 mm; The resistance of layer 131d is measured or estimated from the properties of the material selected for use, the resistance of which is recorded as Rd .
  • the carrier film 131c may be, for example, a nitrocellulose film or a PVDF (polyvinylidene fluoride) film, the specific material type of which is not limited, and the electrical resistance of the carrier film 131c may be measured or estimated according to the characteristics of the material selected for use. The resistance is recorded as R c . It should be noted that the resistance R d of the gel layer 131d and/or the R c of the carrier film 131c vary depending on the material, the amount of the buffer, and the change in temperature.
  • the temperature of the gel layer 131d is, for example, controlled to be lower than 60 ° C to 70 ° C to ensure transfer quality.
  • a constant voltage, a step-up voltage, or a constant current signal is applied to the transfer unit 130 for the transfer operation; Applicants have found that the temperature easily exceeds 60 ° C as the transfer process proceeds or ends.
  • the glue in the transfer unit 130 may even be scorched; and, in order to increase the transfer rate of large proteins, a higher voltage or a larger current is applied. It is not always possible to improve the transfer efficiency, and it is easy to cause the temperature of the transfer unit 130 to be too high, and the transfer quality is difficult to be secured.
  • the control unit 11 in the transfer device 10 is configured to apply undulating unipolar electrical signals to the upper plate electrode layer 1301 and the lower plate electrode layer 1302 of the one or more transfer units 130, the present invention.
  • the unipolar electrical signal is periodically fluctuating according to a predetermined amplitude, wherein a ratio of the instantaneous power corresponding to the highest point of the predetermined amplitude to the instantaneous power corresponding to the lowest point of the predetermined amplitude is greater than or equal to two.
  • the specific size of the predetermined amplitude may be constant or variable, that is, the ratio of the instantaneous power of the highest point of the predetermined amplitude to the instantaneous power of the lowest point corresponding to the predetermined amplitude.
  • the ratio may be constant or varied during a periodic fluctuation of the signal period, for example, relatively constant during one control period, between a plurality of control periods, The signal period of each control cycle is adjustable.
  • the time interval for completing the above-defined fluctuation process is defined as one signal period of the unipolar electrical signal.
  • the time interval at which the highest or lowest point of the above predetermined amplitude occurs continuously is defined as one signal period of the unipolar electrical signal.
  • the unipolar electrical signal is a 10 Hz square wave signal
  • the peak voltage is 25 V and the valley voltage is 5 V at the beginning of the transfer
  • the signal period of the unipolar electrical signal is 100 milliseconds, one signal period.
  • the control unit adjusts the control parameters, and the unipolar electrical signal is still a square wave signal, but the signal frequency of the unipolar electrical signal becomes 20Hz, the peak voltage becomes 20V, and the valley voltage becomes 5V.
  • the one-second electrical circuit is controlled or adjusted, and the predetermined time interval (for example, one second) is the control cycle referred to in this application.
  • the principle of adjusting the unipolar electrical signal during the transfer operation based on the control period will be specifically described later.
  • the instantaneous power corresponding to the highest point of the predetermined amplitude may be referred to as the maximum instantaneous power within the signal period, and the instantaneous power corresponding to the lowest point of the predetermined amplitude may be referred to as the minimum instantaneous period within the signal period.
  • Power; the ratio of the maximum instantaneous power to the minimum instantaneous power during each signal period of the unipolar electrical signal is greater than or equal to about two, and in one embodiment, the ratio is greater than or equal to, for example, 2.5.
  • the unipolar electrical signal defines the amplitude of the undulation change in such a manner that the ratio of the maximum instantaneous power to the minimum instantaneous power in each signal period is greater than or equal to 2, in the corresponding waveform.
  • the high point or peak i.e., the highest point of the predetermined amplitude
  • the low point or trough of the corresponding waveform corresponds to the minimum instantaneous power.
  • the unipolar electrical signal may be a voltage signal or a current signal
  • the unipolar electrical signal may be specifically, but not limited to, a square wave signal, a sine wave signal, a sawtooth wave signal, or a staircase signal, or For their combination; the unipolar electrical signal can be continuous or intermittent.
  • the waveform, frequency, voltage value, current value, and duty ratio of the unipolar electrical signal are important features or parameters that reflect the unipolar electrical signal.
  • the unipolar electrical signal is specifically a square wave voltage signal with adjustable duty ratio, the square wave voltage signal is relatively easy to control and easy to generate, and the duty ratio of the square wave voltage signal can be adjusted by adjusting the pulse width.
  • FIG. 3 is a schematic diagram of a unipolar electrical signal according to an embodiment of the invention, wherein FIG. 3(a) is a square wave voltage signal 91 with adjustable duty ratio, and FIG. 3(b) is FIG. 3(a).
  • An example of a transformation As shown in FIG 3 (a), the peak voltage (i.e., high) is V p, the low level of 0V, V p is greater than or square wave voltage signal is equal to 91 and less than or equal to 1V 30V (e.g. 20V), the corresponding V p the peak current on the transfer unit 130 is greater than or equal to the generated 0.1A and less than or equal to 10A (eg.
  • the signal period T of the square wave voltage signal 91 can be adjusted, even in the same transfer process, specifically by controlling the frequency of the square wave voltage signal 91 to control its signal period T; in one embodiment, The frequency of the wave voltage signal 91 is greater than or equal to 1 Hz and less than or equal to 100 Hz, or for example greater than or equal to 5 Hz and less than or equal to 20 Hz, such as 10 Hz.
  • the duty cycle of the square wave voltage signal 91 is also adjustable, with a duty cycle ranging from 1% to 99%, or for example from 30% to 60%.
  • the square wave voltage signal 91' is a modified embodiment of the square wave voltage signal 91.
  • the voltage peak, the low level, the signal period T and/or Air ratios, etc. can vary.
  • the ratio of the maximum instantaneous power to the minimum instantaneous power in each signal period can be controlled to be greater than or equal to two. It is to be understood that the ratio of the maximum instantaneous power to the minimum instantaneous power in each signal period is not necessarily substantially constant.
  • FIG. 4 is a schematic diagram of a unipolar electrical signal according to still another embodiment of the present invention, wherein FIGS. 4(a) and 4(b) are sinusoidal voltage signals, and FIG. 4(c) and FIG. 4(d) As a triangular voltage signal, FIG. 4(e) and FIG. 4(f) are sawtooth voltage signals, and FIG. 4(g) and FIG. 4(h) are step voltage signals.
  • the signal 92 is a sine half wave voltage signal, which is an intermittent signal, corresponding to a peak voltage V p at the maximum instantaneous power can be generated in each cycle of the signal, corresponding to zero level may be generated at the Minimum instantaneous power.
  • the signal 92 ' is a full-wave sine wave voltage signal, but a minimum voltage greater than or equal to 0, which is a continuous electrical signal, corresponding to a peak voltage V p of the signal may be generated in each cycle
  • the maximum instantaneous power, corresponding to the minimum voltage produces the minimum instantaneous power per signal period.
  • the signal is a triangular wave voltage signal 93, which is an intermittent signal, corresponding to a peak voltage V p at the maximum instantaneous power can be generated in each cycle of the signal, corresponding to the zero level at the minimum instantaneous power may be generated .
  • the signal 93 ' is also a triangular wave voltage signal, but it is a continuous electrical signal, corresponding to a peak voltage V p at the maximum instantaneous power can be generated in each cycle of the signal, corresponding to the minimum voltage (and which Not limited to 0), the minimum instantaneous power in each signal period can be generated.
  • the signal 94 and 94 ' are both sawtooth wave voltage signal, which is an intermittent signal, corresponding to a peak voltage V p at the maximum instantaneous power can be generated in each cycle of the signal, corresponding to The minimum instantaneous power can be generated at the 0 level.
  • the signal 95 is a step voltage signal, which is an intermittent signal, wherein the voltage waveform changes in a stepwise manner when the voltage waveform changes, and the number of steps changes in each signal period, each time. magnitude of the voltage step change were not restrictive; the corresponding peak voltage V p at the maximum instantaneous power can be generated in each cycle of the signal, corresponding to the zero level at the minimum instantaneous power can be generated.
  • FIG. 4(g) the signal 95 is a step voltage signal, which is an intermittent signal, wherein the voltage waveform changes in a stepwise manner when the voltage waveform changes, and the number of steps changes in each signal period, each time. magnitude of the voltage step change were not restrictive; the corresponding peak voltage V p at the maximum instantaneous power can be generated in each cycle of the signal, corresponding to the zero level at the minimum instantaneous power can be generated.
  • the signal 95 ' is also a step voltage signal, a low level but greater than 0 volts, the voltage corresponding to the peak value V p at the maximum instantaneous power can be generated in each cycle of the signal, may be generated at a voltage corresponding to the minimum Minimum instantaneous power per signal period.
  • unipolar electrical signal which is a periodic characteristic; for a voltage signal, and the signal voltage within each cycle, the voltage V p and the minimum peak in each signal period is provided
  • the magnitude of the voltage allows the maximum instantaneous power to minimum instantaneous power ratio in each signal period to be greater than or equal to 2; for current signals, each signal period can be achieved by setting the current peak and minimum current values for each signal period.
  • the ratio of the maximum instantaneous power to the minimum instantaneous power within is greater than or equal to two. It should be understood that the specific waveform of the undulating unipolar electrical signal is not limited to the above embodiment, and may be specifically selected according to specific application requirements.
  • duty cycles For unipolar electrical signals such as sinusoidal, triangular, sawtooth or stepped undulating variations, their respective duty cycles can be defined, for example, the maximum voltage in each signal period corresponds to the average of the minimum voltages.
  • the ratio of the time period greater than the average value to the signal period T is defined as the duty ratio; thus, similarly to the square wave voltage signal 91, their duty ratios can also be adjusted, for example, the range of the duty ratio is 1%-99%, or for example 30%-60%.
  • the control unit 11 is configured to generate the voltage signal of the above embodiment (to generate a square wave voltage signal 91 as shown in FIG. 3(a), which specifically includes the waveform generator 110, the controller 150, and the power supply.
  • Module 190 Human Machine Interface (HMI) 170, real time clock 160, voltage and/or current sensing component 120, and the like.
  • the controller 150 is a core component of the control unit 11, which is capable of outputting control parameters to the waveform generator 110 to generate corresponding unipolar electrical signals, such as square wave voltage signals; the controller 150 can have measurements, calculations, and controls Even the storage function, its specific working principle will be revealed in detail later.
  • the voltage and current detecting component 120 is configured to detect the voltage U and/or the current I applied to the transfer unit 130 in real time and feed it back to the controller 150 as measurement information.
  • the power module 190 is used to supply power to the biotransfer device 10. For example, it can provide AC power or DC power to the waveform generator 110 to generate a unipolar electrical signal of a corresponding waveform, and can also provide a low voltage DC power supply.
  • the controller 150 and through the controller 150, can supply power to the real time clock 160, the voltage and current detecting component 120, and the like that are electrically connected thereto.
  • the real-time clock 160 can provide the current actual time to the waveform generator 110 and the controller 150. Based on the actual time information, the square wave signal having the corresponding frequency (or signal period), duty ratio, and the like can be controlled to be generated; The measurement information received by the device 150 or the self-measured information, such as the voltage or current information fed back by the voltage and current detecting component 120, combined with the actual time information provided by the real-time clock 160, generates measurement information having a corresponding time stamp, so that a pair can be obtained. The time series information of the information should be measured, and the time series information of the measurement information will be used in the calculation process of the control algorithm. Specifically, the real time clock 160 may be embedded in the controller 150.
  • the human-computer interaction interface 170 is used to implement interaction with the user, for example, to implement a user selection or setting of a transfer option, a function of transferring parameters, and a function of starting or stopping the transfer process, and providing user feedback to the controller 150. Function, as well as the ability to present status information to the user during the transfer process.
  • the human-machine interaction interface 170 is not limited to being integrally mounted on the bio-transfer device 10, and may be disposed, for example, separately from the main body of the bio-transfer device 10.
  • control unit 11 further includes a communication unit 180 coupled to the controller 150, by which the control unit 11 can be enabled with external smart terminals (eg, tablets, smart phones, etc.) and/or Alternatively, a cloud computing server or the like may be connected, and some functions of the control unit 11 may be implemented by an external device.
  • the human-machine interaction interface 170 may alternatively be implemented by an external tablet (IPAD) or the like.
  • the corresponding transfer unit 130 is further provided with a temperature sensor 140.
  • the temperature sensor 140 measures the temperature information of the transfer unit 130 in real time, for example, The temperature information of the upper plate electrode layer 1301 and/or the lower plate electrode layer 1302 is measured, and the measured temperature information substantially accurately reflects the temperature of the current gel layer 131d.
  • the temperature information measured by the temperature sensor 140 can be fed back to the control unit 11 as measurement information, for example, to the controller 150.
  • the temperature sensor 140 may be specifically integrated with the transfer unit 130.
  • FIG. 5 shows a control unit 11 of a biotransfer device according to an embodiment of the present invention. Schematic diagram of the functional module structure.
  • the controller 150 controls the waveform generator 110 by outputting control parameters. Therefore, the control unit 11 is provided with a control parameter generation module 151, which can be disposed in the controller 150. Alternatively, it is implemented by the controller 150; the control unit 11 is also provided with a unipolar electrical signal generation module 111, which may be provided in the waveform generator 110 or implemented by the waveform generator 110.
  • the control parameter generating module 151 is configured to generate an adjusted control parameter based on the measurement information or/and the transfer quality information, and the unipolar electrical signal generating module 111 is configured to generate a unipolar electrical signal according to the adjusted control parameter (for example, Square wave voltage signal, etc.).
  • the adjusted control parameter for example, Square wave voltage signal, etc.
  • the control parameter comprises at least one of a waveform, a frequency, a voltage value, a current value and a duty ratio of the unipolar electrical signal;
  • the measurement information is that the upper plate electrode layer 1301 and/or are measured in real time during the transfer process Or temperature information of the lower plate electrode layer 1302, the measurement information being either a waveform including a unipolar electrical signal recorded during the transfer process (for example, a square wave, a sawtooth wave, a triangular wave or a sine wave, etc.), a voltage value Timing information of at least one of U, current value I and duty ratio, and temperature information of the upper plate electrode layer 1301 and/or the lower plate electrode layer 1302.
  • control unit 11 further comprises an input module 154 for receiving input transfer quality information, for example, obtaining a result for the transfer process at the end of the transfer process for a certain transfer unit 130
  • the quality is manually evaluated and input feedback, for example, input quality information is input from the human-machine interface 170 or an external device connected to the communication unit 180, and the input module 154 receives transfer quality information corresponding to each transfer process.
  • the input module 154 can be disposed in the controller 150 or implemented by the controller 150.
  • the user When evaluating the transfer quality information of each transfer process, the user can evaluate according to a predetermined index or standard, and specifically can obtain the transfer quality information expressed by grade or by fraction, for example, based on the following Table 1
  • the print quality rating standard is used to determine the transfer quality information Qt.
  • the elements for evaluating the transfer quality information include, but are not limited to, the transfer efficiency of the print, the condition of the gel layer, the condition of the carrier film, the form of the print, and the condition of the buffer medium layer, and the like, and the respective elements are given corresponding weights.
  • the scoring can be determined by reading the Marker information (for example, the number of imprints remaining on the gel layer 131d); for the condition of the gel layer, it can be burned according to the condition thereof, Curl, good, etc.
  • the overall transfer quality score that is, the transfer quality information Qt, is calculated based on the respective weights.
  • the transfer quality information received each time corresponds to the corresponding transfer unit 130.
  • Each of the plurality of substantially identical transfer units 130 can manually evaluate and input the corresponding transfer quality information Qt after each transfer operation.
  • the transfer quality information Qt is not limited to the determination of the transfer quality information according to the transfer quality scoring standard exemplified above, and the specificity may be adjusted according to the actual application, thereby helping the user to obtain the transfer quality as much as possible. information.
  • control unit 11 further includes a storage module 153 for recording measurement information or/and transfer quality information obtained for each transfer process.
  • the storage module 153 may be disposed in the controller 150 or implemented by the controller 150.
  • the measurement information may be various information obtained by real-time measurement of the transfer process, for example, the temperature of the transfer unit 130, the timing information described above, and the like.
  • the measurement information or/and the transfer quality information recorded by the storage module 153 can be called by the control parameter generation module 151, which of course can also be selectively transmitted by the communication unit 180 to the external device to which it is connected.
  • the storage module 153 may also store corresponding control parameters, such as generated by the control parameter generation module 151, corresponding to the transfer unit 130.
  • the control parameter generation module 151 can directly call the control parameters stored in the historical transfer process stored by the storage module 153, thereby facilitating the rapid completion.
  • the experimental design of the secondary transfer process (for example, including the setting of parameters), the generated undulating unipolar electrical signal will also be applied to the secondary transfer process, facilitating the rapid, high quality completion of the transfer process.
  • the biotransfer device 10 of the embodiment of the present invention is capable of applying a unipolar electrical signal that periodically changes to the transfer unit 130, and the maximum instantaneous power and the minimum instantaneous power in each signal period of the unipolar electrical signal.
  • the ratio is greater than or equal to about 2; at the peak time period or time point corresponding to the maximum instantaneous power, the transfer unit 130 does not continue due to the valley time period or time point at which the power of the voltage or current drop is small in its subsequent time.
  • the magnitude of the voltage or current applied to the gel layer 131d of the transfer unit 130 can be substantially unrestricted.
  • the large instantaneous power of the peak time period or time point can effectively promote the electrophoresis movement of biological macromolecules (especially large masses) such as proteins, ensuring and even improving the transfer efficiency; on the other hand, the trough time
  • the smaller instantaneous power at the segment or time point can effectively prevent the temperature of the transfer unit 130 from rising due to ohmic heat, and the resistance of the transfer unit 130 is also Continues to increase due to heat generation, the temperature of the transfer unit 130 or reducing resistance, also the peak power period of time or point in time in a minor proportion work (i.e., W Tm) for transferring heat radiation unit 130 and the temperature is increased,
  • a large proportion of work ie, transfer effective work WEm
  • WEm transfer effective work
  • the setting of the valley or time point of the smaller power is beneficial to increase the peak time period or time point of the larger power.
  • the transfer performs work efficiency, thereby improving the transfer work efficiency of the unipolar electrical signal as a whole, and the transfer efficiency can be ensured or even improved, and the transfer unit 130 has a slow temperature increase and a good transfer quality during the transfer process.
  • the square wave voltage signal and the constant voltage signal generated by the biotransfer device 10 of the embodiment shown in FIG. 1 are subjected to a transfer effect comparison, wherein the square wave voltage signal is at a high level (ie, the peak voltage Vp).
  • the same as the voltage of the constant voltage signal and the transfer operation time are the same, and the transfer unit 130 in which the square wave voltage signal and the constant voltage signal act is also the same. Need to say It is obvious that in the process of applying a constant voltage signal for the transfer operation, it is necessary to carry out a current limiting corresponding thereto, otherwise an excessive current is likely to be generated to burn the gel layer and the carrier film.
  • the duty ratio of the applied square wave voltage signal is 40%, and when the high level is corresponding, the measured current peak value is 6A in the initial stage of the transfer process, and the measured current peak value is at the end of the transfer process.
  • the temperature of the gel layer (measured by temperature sensor 140) is about 65 °C.
  • the actual applied voltage measured at the initial stage of the transfer process is 18 V, which is measured at the end of the transfer process.
  • the temperature of the gel layer is approximately 78 °C.
  • each test simultaneously performs a transfer operation on the 4 gel layers 131d juxtaposed in the transfer unit 130, and the specific parameters for the two tests for comparison are shown in Table 3 below.
  • the duty ratio of the applied square wave voltage signal is 40%, and when the high level is corresponding, the measured current peak value is 12A in the initial stage of the transfer process, and the measured current peak value is at the end of the transfer process.
  • the temperature of the gel layer (measured by temperature sensor 140) is about 72 °C.
  • the measured actual applied voltage is 15 V at the initial stage of the transfer process, and the measured application is applied at the end of the transfer process.
  • the temperature of the gel layer is approximately 86 °C.
  • the voltage applied to the laminated structure of the transfer unit 130 (including the upper buffer dielectric layer 131e, the gel layer 131d, the carrier film 131c, and the lower buffer dielectric layer 131b) is U, the upper buffer dielectric layer 131e, and the condensation
  • the thickness of the adhesive layer 131d, the carrier film 131c and the lower buffer dielectric layer 131b are respectively d e , d d , d c , d b , and the resistivities are ⁇ , ⁇ d , ⁇ c and ⁇ b , respectively, and the area (perpendicular to the electric field)
  • the cross-sectional area of E is S, and the mobility of the target biomacromolecule in the gel layer 131d and the carrier film 131c is m d and m c , respectively .
  • the above voltage U, thickness d and area S can be regarded as constant values during the transfer process, but their resistivities ⁇ e , ⁇ d , ⁇ c and ⁇ b vary during the transfer process.
  • the change in resistivity is dependent on material properties and temperature, which is due to changes in temperature and/or material composition.
  • the mobility rates m d and m c depend on the size and shape of the target biomacromolecule, and the structure of the gel layer 131d and the carrier film 131c (e.g., the size of the pores) and the material, and the upper buffer dielectric layer 131e and the lower buffer dielectric layer 131b. Chemical and physical characteristics, etc.
  • 131d gel layer, the carrier film 131c, 131e on the buffer layer and the lower dielectric layer is buffered media of resistance R d 131b, R c, R b and R e may be by the following relationship (1-1-1), (1 -1-2), (1-1-3), (1-1-4) respectively indicate:
  • R d , R c , R b and R e are the electrical resistances of the gel layer 131d, the carrier film 131c, the upper buffer dielectric layer 131e and the lower buffer dielectric layer 131b, respectively.
  • the voltage U d is a voltage applied to the gel layer 131d
  • E d is an electric field intensity applied to the gel layer 131d.
  • transfer rate v of the target biomacromolecule is further expressed based on the following relationship (1-3):
  • m is the mobility of the biomacromolecule.
  • R b (T) R b ⁇ [k ⁇ ( ⁇ - ⁇ )2+1] (1-6-1)
  • R b (T) and R b (T) represent the resistance of the upper buffer dielectric layer 131e and the lower buffer dielectric layer 131b under the temperature T condition, respectively;
  • k is a heating coefficient (for example, it can be estimated as 10);
  • ⁇ is occupied a threshold value indicating that the duty ratio of the square wave voltage signal is equal to the duty cycle threshold, the temperature of the transfer process is no longer increased, for example, it may be equal to 20%; and
  • R b and R e are upper buffer dielectric layers, respectively The resistance of 131e and lower buffer dielectric layer 131b before the start of transfer.
  • the transfer effective work WE is a watershed, that is, the transfer effective work or the transfer work efficiency is relatively The peak value is reached; for the square wave signal used in the numerical simulation (such as the square wave signal shown in Figure 3(a)), the 50% duty cycle is equivalent to 50% of the full power output (the full power output corresponds to the duty cycle). 100% power output), that is, for other waveforms of unipolar electrical signals, it is necessary to achieve 50% or less (including 50%) full power output by adjusting the duty ratio in the range of 1%-99%. . If the ratio of the maximum instantaneous power P max to the minimum instantaneous power P min in the signal period is less than 2, it is assumed that the power output P is equivalently calculated based on the following relation (1-8):
  • the adjusted power output P cannot be output to 50% or less (including 50%) of the full power output.
  • the ratio of the maximum instantaneous power P max to the minimum instantaneous power P mi n in the signal period of the unipolar periodic signal is greater than or equal to about 2, that is, unipolar
  • the electrical signal periodically fluctuates according to a predetermined amplitude, and the ratio of the instantaneous power of the highest point of the predetermined amplitude to the instantaneous power of the lowest point of the defined amplitude is greater than or equal to 2; thus, the power output P can be achieved by adjusting the duty ratio ⁇ 50% or less of the full power output, that is, the transfer effective work or the transfer work efficiency can be relatively peaked.
  • the ratio of the maximum instantaneous voltage in the set signal period to the minimum instantaneous voltage is greater than or equal to about
  • the ratio of the large instantaneous power P max to the minimum instantaneous power P mi n can be greater than or equal to about 2;
  • the ratio of the maximum instantaneous current x to the minimum instantaneous current in the set signal period is greater than or equal to approximately Greater than can be achieved instantaneous power P max and the minimum instantaneous power P mi n is greater than or equal to about 2.
  • Fig. 8 further exemplifies the control principle of the biotransfer device, and from this point of view, the technical effects of the biotransfer device 10 of the above embodiment of the present invention can also be exemplarily explained.
  • the example illustrates how the duty ratio ⁇ generated by the control parameter generation module 151 of the controller 150 and the same transfer are adjusted based on the measurement information of the temperature information T including the transfer unit 130 measured in real time during the primary transfer process.
  • the unit 130 adjusts the control parameters based on the transfer quality information Qt during the multiple transfer process, thereby maximizing the transfer work efficiency.
  • the square wave voltage signal will be described below by way of example with reference to FIG.
  • the transfer effective work WEm is maximized by dynamically adjusting the duty ratio ⁇ of each control period in real time.
  • W m represents the total work done by the square wave voltage signal during transfer
  • W Tm represents the work consumed for heat radiation and temperature increase during the transfer process
  • W m can be calculated by the following relation (2-2):
  • ⁇ t is the time interval of the first control loop process 191, which corresponds to one control period, one control period is greater than or equal to one signal period, for example, it corresponds to a plurality of signal periods having unipolar electrical signals;
  • m represents the mth
  • U m represents the voltage amplitude measured by the mth transfer process (if the minimum voltage of the unipolar electrical signal in the control period is equal to 0, the voltage amplitude represents the voltage peak)
  • I i represents the ith control
  • the current amplitude obtained by the period measurement if the minimum current in the control period is equal to 0, the current amplitude represents the peak value of the electric current
  • ⁇ i represents the duty ratio of the electrical signal in the ith control period (the unipolar electricity in the control period)
  • the duty cycle of the signal is constant, that is, both ⁇ i ), 1 ⁇ i ⁇ (n-1).
  • W Tm can be calculated by the following relation (2-3):
  • ⁇ m represents the equivalent thermal emissivity of the transfer unit 130 during the mth transfer
  • C represents the specific heat capacity of the transfer unit 130
  • T i and T i-1 represent the i and i (i-1), respectively
  • the temperature information of the obtained transfer unit 130 is measured by a control period.
  • the temperature information T and the current information I measured in the first to (n-1)th control periods based on the square wave voltage signal are input to the above relation (2-5), and are calculated in the first to W em maximized so that the duty ratio corresponding to the first (n-1) th control period, and the duty ratio of the duty output as [mu] n the n-th control cycle, i.e., corresponding to the n-th control period
  • the duty ratio ⁇ n in the control parameter is used, and the waveform generator 110 adjusts the duty ratio of the next control period (i.e., the nth control period) based on the duty ratio ⁇ n . It is foreseen that when the square wave voltage signal of the nth control period based on the duty ratio ⁇ n continues the transfer operation, the Wem can continue to be maximized.
  • i represents the i-th transfer process of the first to (m-1)th transfer processes
  • m is an integer greater than or equal to 2
  • Q ti represents transfer quality information corresponding to the i-th transfer process
  • W ei represents the transfer effective work of the i-th transfer process, which can be calculated by the above relation (2-5).
  • the equivalent thermal emissivity ⁇ m-1 can be obtained by fitting calculation. Further, the equivalent thermal emissivity ⁇ m-1 is used as the intermediate input.
  • the transfer effective work Wem is the maximum value when the relative equivalent heat radiation coefficient ⁇ is used as an independent variable.
  • the equivalent thermal radiation coefficient ⁇ obtained in the second control loop process 192 above may also be substituted into the relation (2-5) for obtaining the corresponding duty in the first control loop process 191.
  • the specific parameters are obtained to obtain the maximum transfer effective work.
  • the above first control loop process 191 and second control loop process 192 are both for achieving the largest possible transfer work efficiency, thereby reducing the work consumed for heat radiation and temperature increase during the transfer process, which is advantageous for reducing The temperature of the transfer unit 130 increases the transfer quality.
  • the control parameter generation module 151 of the controller 150 can automatically optimize other control parameters other than the duty cycle, such as frequency or period, voltage
  • the control parameter generation module 151 of the controller 150 has a self-learning function.
  • a self-learning function can function in, for example, for example, a newly used biotransfer device 10, a new transfer unit 130 (eg, a new gel layer 131d, a carrier film 131c, and/or an upper and lower buffer dielectric layer).
  • control parameter generation module 151 of the controller 150 may use a kernel fitting algorithm to perform calculation or optimize control parameters, and the kernel fitting algorithm uses an artificial neural network, which may specifically but not limited to a supervised learning network ( Supervised Learning Network), Hybrid Learning Network, Reinforcement Learning Network, Hopfield Network, Boltzmann Machine, Stochastic Neural Networks )Wait.
  • the neural network data can be trained by setting different input variables to optimize different control parameters.
  • FIG. 9 is a schematic view showing the structure of a biotransfer device in accordance with a second embodiment of the present invention.
  • the biotransfer device 20 is similarly provided with a waveform generator 110, a voltage and current detecting part 120, a transfer unit 130, a temperature sensor 140, and a control similar to those in the biotransfer device 10 shown in FIG. 1.
  • a waveform generator 110 a voltage and current detecting part 120
  • a transfer unit 130 e.g., a thermoelectric transfer unit 130
  • a temperature sensor 140 e.g., a thermoelectric transducer
  • the power module provided in the bio-transfer device 20 190 has a relatively different implementation.
  • the power module 190 is coupled by electromagnetic coupling.
  • Energy transfer is achieved, which includes a primary unit 191 and a secondary unit 192 such that the biotransfer device 20 can be made to contact power in a non-contact manner. Therefore, the structure of the biotransfer device 20 in the embodiment shown in FIG. 9 can be designed to be more compact, and it is easier to achieve overall waterproof, biological or chemical contamination (for example, reducing metal contamination of biological agents and the like due to conductive contact). ), more convenient and flexible to use, very suitable for use in biological laboratories.
  • FIG. 10 is a schematic view showing the structure of a biotransfer device in accordance with a third embodiment of the present invention.
  • the biotransfer device 20 is similarly provided with a waveform generator 110, a voltage and current detecting part 120, a transfer unit 130, a temperature sensor 140, and a control similar to those in the biotransfer device 10 shown in FIG. 1.
  • a waveform generator 110 a voltage and current detecting part 120
  • a transfer unit 130 e.g., a thermoelectric transfer unit 130
  • a temperature sensor 140 e.g., a thermometer
  • One or more of the device 150, the real-time clock 160, the human-machine interaction interface 170, and the communication unit 180, and details are not described herein again.
  • the biotransfer device 20 mainly has the following differences with respect to the biotransfer device 10: (1) The biotransfer device 20 is structurally divided into a control end 310 and a transfer end 320, and the control end 310 and the transfer end 320 are separated. (2) The power module 190 has a relatively different implementation. In particular, energy and/or information transmission is achieved between the control terminal 310 and the transfer terminal 320 by non-contact electromagnetic coupling.
  • the control terminal 310 is mainly used to implement the function of the control unit, which is provided with a waveform generator 110, a voltage and current detecting component 120, and a controller similar to those in the biotransfer device 10 as shown in FIG. 1. 150.
  • the control terminal 310 is further provided with an alternating current-direct current (AC-DC) conversion unit 193 for supplying power to various components.
  • the AC-DC conversion unit 193 is primarily used to convert the AC power of the grid to a corresponding level of DC power, which is part of the power module.
  • the unipolar electrical signal (eg, a square wave voltage signal) generated by the control terminal 310 for application on the transfer unit 130 is wirelessly transmitted to the transfer end 320, for example, may be simultaneously transmitted to multiple (eg, x, x) Is an integer greater than or equal to 2) transfer ends 320 1 , 320 2 ... 320 x .
  • a temperature sensor 140 is also integrally provided.
  • a primary unit 191 is correspondingly disposed on the control terminal 310.
  • one control terminal 310 is provided with x (x ⁇ 2) primary units 191 1 , 191 2 ... 191 x .
  • the unipolar electrical signal generated by the control terminal 310 can be transmitted to the plurality of transfer ends 320 by non-contact electromagnetic coupling, that is, to achieve non-contact transfer of energy, and on the other hand, information generated by each transfer end 320.
  • the temperature information collected by the temperature sensor 140 is transmitted to the control terminal 310 through the non-contact electromagnetic coupling, that is, the information is transmitted non-contactly.
  • FIG 11 is a block diagram showing the structure of a non-contact electromagnetically coupled power supply module used in a biotransfer device in accordance with an embodiment of the present invention.
  • the power module 190 in the biotransfer device 30 includes a primary unit 191 and a secondary unit 192, a primary coil 1911 and a magnetic axis 1912 are disposed in the primary unit 191, and a secondary coil 1921 is disposed in the secondary unit 192.
  • the primary unit 191 is provided.
  • the secondary unit 192 can be separately wrapped, for example, by a plastic shell.
  • the structure of the transfer end 320 can be designed to be more compact, and it is easier to achieve waterproof design, biological or chemical contamination (for example, reducing biological agents caused by conductive contact). It is more convenient and flexible to use, which is very suitable for application in biological laboratories.
  • Fig. 12 is a view showing the configuration of a biotransfer system of a first embodiment formed based on the biotransfer device of the embodiment shown in Fig. 9.
  • the biotransfer system includes a plurality of biotransfer devices 20, and their control units are coupled to the cloud server 90, so that the cloud server 90 and any one of the biotransfer devices 20 can be realized.
  • Information or data transmission for example, measurement information and/or transfer quality information obtained in the biotransfer device 20, etc., may be uploaded to the cloud server 90, and the cloud server 90 may also transmit information (eg, control parameters) to the corresponding biotransfer. Device 20.
  • n (n ⁇ 2) biotransfer devices 20 1 , 20 2 . . . 20 n may be simultaneously communicatively coupled to a portable smart terminal 175, for example, via communication unit 180 of biotransfer device 20
  • the portable smart terminal 175, the portable smart terminal 175 may specifically be an tablet (IPAD), a smart phone, etc., and the portable smart terminal 175 may implement the function of the human-machine interaction interface 170 of the bio-transfer device 20 in whole or in part, for example, by A corresponding APP application is installed thereon to effect interaction with each of the biotransfer devices 20, particularly the controller 150.
  • a corresponding APP application is installed thereon to effect interaction with each of the biotransfer devices 20, particularly the controller 150.
  • the cloud server 90 can be communicatively coupled with m (m ⁇ 2) portable smart terminals 175 1 ... 175 m to implement any bio-transfer connected to the m portable smart terminals 175 1 ... 175 m
  • the printing device 20 is coupled; in yet another alternative embodiment, the cloud server 90 can also be in direct communication with the transfer device 20.
  • Fig. 13 is a view showing the configuration of a biotransfer system of a second embodiment formed based on the biotransfer device of the embodiment shown in Fig. 10.
  • the cloud server 90 of the biotransfer system is communicatively coupled with m (m ⁇ 2) portable intelligent terminals 175 1 ... 175 m , each of which has n (n ⁇ 2) biotransfers.
  • the devices 30 1 , 30 2 ... 30 n are communicatively coupled.
  • FIG. 14 is a block diagram showing the structure of a cloud server of a biotransfer system according to an embodiment of the present invention.
  • the cloud server 90 is provided with a history database 910, a cloud computing module 920, and a communication module 930; wherein the history database 910 can store data sent by each biotransfer device, and the history database 910 can have at least multiple
  • the measurement information or/and the transfer quality information record obtained by each of the biotransfer devices 20 or 30 at each transfer process is stored as historical data information (for example, data stored by the storage module 153 of each biotransfer device).
  • the cloud computing module 920 can perform cloud computing or big data analysis processing on the historical data, and specifically generate a current turn corresponding to one of the plurality of biotransfer devices (10 or 20 or 30) based on the historical data information.
  • the control parameters of the printing process thereby facilitating the use of historical data information to quickly determine the control parameters in accordance with the current transfer process, greatly improving the user experience .
  • the cloud computing module 920 can use various cloud technologies to enhance the robustness of the intelligent control during the calculation and analysis process, and the history database 910 can selectively store the measurement information corresponding to the transfer process with better transfer quality information. And / or control parameters, in the cloud computing process, which can accelerate the efficiency of self-learning to obtain the best control parameters and reduce the workload of cloud computing.
  • the cloud computing module 920 may also completely or partially replace the functions of the control parameter generation module 151 in the completion controller 150.
  • control parameters obtained by the cloud computing module 920 can be sent to the control unit of the corresponding biotransfer device 10, 20 or 30 via the transmitting module 193.
  • the biotransfer device 10, 20 or 30 can generate or update a corresponding unipolar electrical signal based on the control parameters.
  • the cloud server 90 due to the introduction of the cloud server 90, the sharing of historical data information of many biotransfer devices is realized, and the calculation parameters of the historical data information are obtained by the cloud computing module 920, which is equivalent to realizing the setting control of each user.
  • the experience of parameters is shared and exploited, greatly reducing the professional skills and experience of users or experimenters. begging.

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Abstract

L'invention concerne un dispositif de transfert par buvardage (10), un système de transfert par buvardage et un procédé de commande. Le dispositif de transfert par buvardage (10) comprend une unité de commande (11) et une ou plusieurs unités de transfert par buvardage (130). L'unité de commande (11) est conçue pour appliquer à une première couche d'électrode plane et à une seconde couche d'électrode plane desdites unités de transfert par buvardage (130) un signal électrique monopolaire, le signal électrique monopolaire variant selon une période d'amplitude prédéterminée, un rapport d'une puissance instantanée correspondant au pic de l'amplitude prédéterminée à une puissance instantanée correspondant au point le plus bas de l'amplitude prédéterminée étant supérieur ou égal à 2. Le dispositif de transfert par buvardage présente une efficacité de transfert par buvardage supérieure et garantit une excellente efficacité et une excellente qualité de transfert.
PCT/CN2017/081591 2017-04-24 2017-04-24 Dispositif de transfert par buvardage, système de transfert par buvardage et procédé de commande WO2018195682A1 (fr)

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