WO2018189610A1 - Anti-sprouting mixture comprising essential oils - Google Patents

Anti-sprouting mixture comprising essential oils Download PDF

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Publication number
WO2018189610A1
WO2018189610A1 PCT/IB2018/052180 IB2018052180W WO2018189610A1 WO 2018189610 A1 WO2018189610 A1 WO 2018189610A1 IB 2018052180 W IB2018052180 W IB 2018052180W WO 2018189610 A1 WO2018189610 A1 WO 2018189610A1
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origanum
essential oil
spp
mentha
eugenia caryophyllata
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PCT/IB2018/052180
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French (fr)
Inventor
Sergio Fulceri
Sara MERCATI
Lorenzo GRIFONI
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Aboca S.P.A Società Agricola
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Priority to EP18717433.9A priority Critical patent/EP3609333A1/en
Publication of WO2018189610A1 publication Critical patent/WO2018189610A1/en

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/22Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint

Definitions

  • Mentha spicata oil With regard to Mentha spicata oil, it is also reported in the literature (Teper- Bamolker et al, "Mint essential oil can induce or inhibit potato sprouting by differential alteration of apical meristem.” Planta. 2010 Jun;232(1): 179-86.) that the aforesaid oil exhibits an effect - inhibiting - potato sprouting. The document, in fact, teaches that, depending on the concentration used for treatment on tuber, Mentha spicata oil has effects inhibiting sprouting in potato or inducing the same.
  • the present description also relates to an anti-sprouting product comprising said composition in suitable quantities and in suitable vessels, the use of said composition or anti-sprouting product to inhibit the sprouting of axillary and/or adventitious buds in flowered or topped plants, or to inhibit the growth of young seedlings at the stage between the first and the fourth leaf, a method to inhibit the sprouting of axillary and/or adventitious buds in herbaceous stem plants or in shrubs, comprising one or more treatment steps of treating said plants with said composition or said anti-sprouting product, and a process for the preparation of said composition.
  • composition could be as follows:
  • the invention further relates to a process for the preparation of a composition as defined in any one of claims 1 to 15, comprising one or more steps of mixing of at least two of

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Agronomy & Crop Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention relates to a plant growth regulating composition, able to inhibit the sprouting of axillary or adventitious buds in herbaceous plants, trees and shrubs, comprising as active ingredients fixed quantities of at least two of Mentha spp. essential oil, Eugenia caryophyllata essential oil and Origanum spp. essential oil; products comprising said composition, use of the composition and new methods to inhibit the sprouting of axillary or adventitious buds in herbaceous stem plants and in shrubs.

Description

ANTI-SPROUTING MIXTURE COMPRISING ESSENTIAL OILS
DESCRIPTION
The present invention relates to a plant growth regulating composition, able to inhibit the sprouting of axillary or adventitious buds in herbaceous plants, shrubs and trees, comprising as active ingredients fixed quantities of Mentha spp. essential oil in combination with Origanum spp. essential oil and/or Eugenia caryophyllata essential oil; products comprising said composition, use of the composition and new methods to inhibit the sprouting of axillary or adventitious buds in herbaceous stem plants and in shrubs. STATE OF THE PRIOR ART
In many cases, both in agriculture and in the production of plants or flowers for ornamental purposes, the sprouting of axillary and/or adventitious buds in the aerial part of the plant is not desired.
Moreover, the control of such development can be particularly awkward in plants subjected to topping.
A typical example is tobacco cultivation, in which topping is a fundamental technique for the quality of leaves present on the plant.
This technique is adopted at a definite development stage of the in-field plant and has the side effect of causing the elimination of the so-called "apical dominance". In fact, as is known, the apical bud of Tobacco (accountable for plant growth in height and ending its physiological activity when floral scape shooting is had), during its growth, has a role of inhibitor on all other buds found therebelow (axillary buds, as present at the axil between petiole (leaf stalk) and main stalk) keeping them quiescent. Topping, which is carried out just by removing with a clean cut the main apical bud, thereby obtaining a "topped plant"; remarkably affects the chemical and physical nature of the produce; in tobacco varieties requiring topping, it is carried out to reduce the number of leaves to be harvested and to let accumulate in the remaining ones a right content of starch, which in subsequent care transforms into sugars, nicotine and aromatic substances.
Post-topping, a control of buds (shoots) originating from axillary buds (buds located at leaf axils), and which normally develop because of apical dominance elimination, needs to be carried out. In fact, the presence of buds causes yield reductions and quality (grade) deterioration due to low presence of starch, and accordingly of reducing sugars, in leaves. The presence of buds, moreover, hinders mechanical harvesting.
In tobacco growing, bud (shoot) controlling technique is based on the use of contact- acting and systemic-acting plant growth regulators, to be used according to different modes:
Treatments fractionated into two interventions: first intervention: with fatty alcohol-based contact-acting products, to be used diluted in water, at the dose of 4-5% of commercial formulate; usually requiring 400- 600 liters of water/ha. The treatment should be carried out on dry plants, avoiding windy days and the hottest hours of the day; second intervention: to be carried out after 5-8 days, with a maleic hydrazide-based systemic-acting product, to be used diluted in water at a concentration not higher than 3% for commercial formulates containing 15% of active ingredient (a.i.), or not higher than 0.8% for commercial formulates containing 60% of a.i.; this, using volumes of 400-500 liters of water/ha. This operation must be carried out with the tobacco in full turgor. Raining within 6 hours from treatment can compromise its effectiveness.
Single treatment: a single-treatment technique can be used as well, by using a mixture of a contact- acting product plus a systemic-acting product to carry out the intervention in a single step.
Alternatively, treatments fractionated into three interventions can be carried out:
Use of fatty alcohol-based contact-acting products, to be used in successive interventions, at 5-7 days from each other and with solutions at increasing concentrations of commercial formulate:
1- 1.5% first intervention;
2- 2.5% second intervention;
3- 3.5% third intervention This methodology can prove to be sufficient for a good control of buds (sprouting) in case of early varieties, whereas in case of more late varieties it may be necessary to intervene with a last treatment with a systemic-acting product (maleic hydrazide), at the aforeindicated doses.
Were an "anti-sprouting treatment" by use of plant growth regulators as above- indicated not to be carried out, the tobacco grower would be forced to carry out a so- called "manual pruning", i.e., the manual removal of axillary buds that, after topping, would grow vigorous at leaf axils and, when left to their normal development, would cause the loss of positive characteristics of the leaves, object of the harvesting, the non-forming of aromatic substances thereon, and the non-reaching of ripening, with lack of consistency of the "leaf skin", above all in Dark Tobaccos. Evidently, this manual operation is unsuitable from the standpoint of costs, due to the demand for labour required to carry it out; therefore, the use of specific plant growth regulators, limiting, preventing, inhibiting the development of axillary buds is virtually mandatory.
In agriculture and floriculture there are other cases in which the development of buds below the apical bud is undesired, and in which agricultural techniques and/or plant growth regulators are used.
Currently, the sole example of anti-sprouting plant growth regulator of natural origin is a product that was registered for stored potatoes, with formula, in a thermal spraying solution, of Mentha spicata oil (high L-carvone content - 60 to 80%). The product consists of 100% Mentha oil.
Assays carried out by the Authors of the present invention showed the non- applicability of the product on the aerial part of assayed plants.
Given emerging human attention to a greater respect for the environment and for the health of plants, animals and human beings, the interest for the development of products of natural origin is ever greater; in this case, the development of products of natural origin that may have activities as anti-sprouting plant growth regulators to be used in organic agriculture techniques is of utmost interest.
SUMMARY OF THE INVENTION
The Authors of the present invention tried using Mentha spicata oil, currently used as anti-sprouting plant growth regulator on potato tubers during their storage, on herbaceous stem plants, finding it exerts a marked phytotoxicity, proving markedly phytotoxic when administered in water, producing burns and maculations of leaves, where contacted by the product, and lethal when administered in sunflower oil (non- phytotoxic, not a plant growth regulator), when used at the concentrations for which effectiveness on potato is reported in the literature.
With regard to Mentha spicata oil, it is also reported in the literature (Teper- Bamolker et al, "Mint essential oil can induce or inhibit potato sprouting by differential alteration of apical meristem." Planta. 2010 Jun;232(1): 179-86.) that the aforesaid oil exhibits an effect - inhibiting - potato sprouting. The document, in fact, teaches that, depending on the concentration used for treatment on tuber, Mentha spicata oil has effects inhibiting sprouting in potato or inducing the same. From the publication, it emerges that tubers treated with Mentha oil at dosages of about 0.5 microl l(-1) catalyzed axillary bud sprouting in potato tubers, whereas dosages of about 4.5 microl l(-1), effectively inhibited sprouting of said buds. The Authors of the present invention have analyzed the axillary bud sprouting regulation (phytoregulatory) effectiveness and the phytotoxicity of various plant essential oils, and have surprisingly discovered that a composition comprising Mentha spp. essential oil and Origanum spp. essential oil, or Mentha spp. essential oil and Eugenia caryophyllata essential oil, or a mixture of the three essential oils at suitable concentrations, has a very effective effect of inhibiting axillary bud development and exhibits no phytotoxicity when used on herbaceous stem plants or on shrubs. Surprisingly, the suitable concentrations of Mentha spp. essential oil selected by the Inventors are concentrations reported in the literature as ineffective, or even inducing the development of potato axillary buds (Teper-Bamolker et al, "Mint essential oil can induce or inhibit potato sprouting by differential alteration of apical meristem." Planta. 2010 Jun;232(1): 179-86.) and Origanum essential oil is reported in the literature as ineffective in the inhibition of axillary bud sprouting in potato (in Vokou et al "Effects of aromatic plants on potato storage: sprout suppression and antimicrobial activity", Agriculture, Ecosystems & Environment Volume 47, Issue 3, December 1993, Pages 223-235). The Authors of the invention are not aware of data, in the literature on essential oils, on 0.1-2% w/w Mentha spp. essential oil
0.1-2% w/w Origanum spp. essential oil, in connection with plant bud sprouting.
The composition proposed by the Authors of the invention has instead a high anti- sprouting effectiveness and does not exhibit a relevant phytotoxicity for the plant treated.
Therefore, object of the invention is a composition comprising, as active ingredients,
0.1-2% w/w each, of at least two of Mentha spp. essential oil, Origanum spp. essential oil and Eugenia caryophyllata essential oil, and at least one phytopharmacologically acceptable co-formulating agent; object of the invention are also an anti-sprouting (or "sprouting-suppressing") product comprising said composition in suitable quantities and in suitable vessels, the use of said composition or anti-sprouting product to inhibit the sprouting of axillary and/or adventitious buds in flowered or topped plants, or to inhibit the growth of young seedlings at the stage between the first and the fourth leaf, a method to inhibit the sprouting of axillary and/or adventitious buds in herbaceous stem plants or in shrubs, comprising one or more treatment steps of treating said plants with said composition or said anti-sprouting (sprouting-suppressing) product and a process for the preparation of said composition.
DETAILED DESCRIPTION OF THE INVENTION
As indicated above, the invention relates to a composition comprising
0.1-2% w/w each, of at least two of Mentha spp. essential oil, Origanum spp. essential oil and Eugenia caryophyllata essential oil, and at least one phytopharmacologically acceptable co-formulating agent.
The concentration expressed in w/w indicates the weight of each component with respect to the total weight of the composition, meant as 100%, and refers to the composition meant as "ready for use". Concentrated preparations whose use is intended as that described herein fall within the object of the invention.
In other terms, the composition of the invention comprises:
0.1-2% w/w of Mentha spp. essential oil
0.1-2% w/w of Origanum spp. essential oil, or
0.1-2% w/w of Mentha spp. essential oil
0.1-2% w/w of Eugenia caryophyllata essential oil, or
0.1-2% w/w of Origanum spp. essential oil, and
0.1-2% w/w of Eugenia caryophyllata essential oil, or 0.1-2% w/w of Mentha spp. essential oil
0.1-2% w/w of Origanum spp. essential oil
0.1-2% w/w of Eugenia caryophyllata essential oil and at least one
phytopharmacologically acceptable co-formulating agent.
In a further embodiment, the composition described herein comprises
0.1-2% w/w of Mentha spp. essential oil
0.1-2% w/w of Origanum spp. essential oil, or
0.1-2% w/w of Mentha spp. essential oil
0.1-2% w/w of Eugenia caryophyllata essential oil, or
0.1-2% w/w of Origanum spp. essential oil, and
0.1-2% w/w of Eugenia caryophyllata essential oil, or
0.1-1 % w/w of Mentha spp. essential oil
0.1-1 % w/w of Origanum spp. essential oil
0.1-1 % w/w of Eugenia caryophyllata essential oil, and at least one
phytopharmacologically acceptable co-formulating agent.
Object of the invention are also concentrates of the above-described composition (meant as "mother") to be diluted at the above- reported concentrations for on-plant use. Such concentrates could be, for instance, at concentrations enabling a more practical storage and a convenient nx dilution (e.g., in water or in an aqueous carrier) like, e.g., 2x, 5x, 10x, 20x, 25x, 30x, 35x, 40x, 45x, 50x, etc.
The wording nx (n meant as integer between 2 and 50), refers to the number of times by which the composition is concentrated with respect to the final product to be used on-plant, as is common in chemistry. Therefore, a 2x composition should be diluted two times, whereas a 50x composition should be diluted 50 times before being used on-plant.
Hence, object of the invention is a composition according to any one of the embodiments described and claimed herein in a 2x, 5x, 10x, 20x, 25x, 30x, 35x, 40x, 45x or 50x concentrated form, wherein said composition in concentrated form should be diluted at the final 1x concentration prior to use on plant.
The term phytopharmacologically acceptable co-formulating agent can be replaced in any part of this description and of the claims by the term "phytopharmacologically acceptable excipient".
By phytopharmacologically acceptable co-formulating agent or excipient it is meant a substance that may be added to the above-indicated mixture of essential oils and that does not exert per se an anti-sprouting (sprouting-suppressing) effect.
According to the present description, phytopharmacologically acceptable tackifying agents, fixing agents, surfactants, emulsifiers, carriers, and anyhow agents commonly used by the technician in the field for the making of compositions adapted to be administered to the aerial part of plants, fall within the definition of co- formulating agents or excipients, without being limited thereto.
The present description also relates to an anti-sprouting product comprising said composition in suitable quantities and in suitable vessels, the use of said composition or anti-sprouting product to inhibit the sprouting of axillary and/or adventitious buds in flowered or topped plants, or to inhibit the growth of young seedlings at the stage between the first and the fourth leaf, a method to inhibit the sprouting of axillary and/or adventitious buds in herbaceous stem plants or in shrubs, comprising one or more treatment steps of treating said plants with said composition or said anti-sprouting product, and a process for the preparation of said composition.
For the purposes of the present description, the term "inhibitor of the sprouting of axillary or adventitious buds" denotes a substance, a composition, a mixture, a product, with a plant growth regulating effect, preventing or retarding, with respect to plants not treated with said inhibitor, the development of axillary or apical buds (sprouts) from the related buds.
This term may be used in replacement of, or be replaced by the term "anti- sprouting" ("sprouting-suppressing").
"Of natural origin", for the purposes of the present description, refers to substances or mixtures of substances extracted, derived, obtained from natural products, like, e.g., from plants, and therefore to products that are not obtained by chemical synthesis. By Mentha spp., for the purposes of the present description, are meant all species belonging to the Mentha genus.
By Origanum spp., for the purposes of the present description, are meant all species belonging to the Origanum genus. The term Eugenia caryophyllata, for the purposes of the present description, can be replaced in any point by the term Syzygium aromaticum, in the literature referring both to the same plant belonging to the Myrtaceae family.
As active ingredients, for the purposes of the present description, are meant substances, extracts, oils, mixtures or groups of substances exerting the desired plant growth regulating effect, i.e. the effect of inhibiting the development of axillary or adventitious buds in herbaceous stem plants or in shrubs.
The wording "phytopharmacologically acceptable" denotes a compound suitable for use in phytopharmacology, i.e., a compound that may be introduced into a plant protection product, where by plant protection product is meant a composition that, by intervening on the biological cycle of a plant, causes thereto changes apt to enhance and improve the production of the plant itself, or is used as a drug for plant disease treatment. A phytopharmacologically acceptable substance is therefore a substance that may be introduced into a plant protection product and that therefore is not harmful to plant health.
The wording "herbaceous plants" refers to vegetables without lignification, whereas the wording "shrub plants" refers to plants lacking a main stem, i.e. a perennial stem branching out right off the base, or even plants with a shrubby bearing. A non- limiting example of common shrubs consists of rose, hawthorn, viburnum, elder and the like. "Shrub plant" therefore denotes a plant with a shrub-like bearing. The wording "tree plants" refers to plants with a lignified stem.
The Authors of the present invention have, as mentioned above, made a composition with a plant growth regulating effect of inhibiting the sprouting of axillary or adventitious buds in herbaceous plants, shrubs and trees, by singling out a specific mixture and concentration of essential oils having a very effective anti- sprouting effect and concomitantly a low phytotoxicity.
As reported above, the Authors of the invention verified that the use of Mentha spicata oil as per literature on potato was not applicable to the aerial part of herbaceous plants, shrubs and trees during their growing. The Authors in fact observed that Mentha spicata oil, at the 4% w/w concentration suggested in the literature and indicated for the treatment of potato tubers, has, when administered in oily carriers, a lethal effect on plants, and, when administered in aqueous carriers, a markedly phytotoxic effect, with relevant damage to treated plant. Said effects ruled out the possibility of being able to use what commonly used on potato, on plants such as tobacco after topping, or on other plants in which it is desirable to inhibit the development of axillary or adventitial buds.
The Authors of the invention therefore assayed some plant essential oils in order to identify possible candidates for the development of anti-sprouting plant growth regulators suitable for use in conventional and organic agriculture.
As reported hereinafter and in the experimental section, the Inventors have identified particularly suitable combinations and concentrations of essential oils, resulting in a robust anti-sprouting activity free from undesired side effects on plant health.
Accordingly, object of the present invention is a plant growth regulating composition, with an anti-sprouting effect, i.e., of inhibiting the sprouting of axillary and/or adventitious buds, comprising, as active ingredients, i.e. as ingredients accountable for the aforesaid plant growth regulating activity, said composition comprising, as anti-sprouting ingredients,
0.1-2% w/w each, of at least two of
Mentha spp. essential oil
Origanum spp. essential oil and
Eugenia caryophyllata essential oil and
at least one phytopharmacologically acceptable co-formulating agent as indicated above.
As mentioned above, said co-formulating agent can be, e.g., represented by any one of phytopharmacologically acceptable tackifying agents, fixing agents, surfactants, emulsifiers, carriers. As mentioned above, according to the present invention, Mentha spp. essential oil denotes essential oil extracted from plants of any species belonging to Mentha genus, or even a mixture thereof. Among these, for the purposes of the present invention, there can be used essential oils of one or more plants, or hybrids thereof, selected from the group comprising, e.g., Mentha aquatica, Mentha germanica, Mentha arvensis, Mentha citrata, Mentha bergamot, Mentha gentilis, Mentha aurea, Mentha piperita, which is a hybrid between Mentha aquatica and Mentha viridis, Mentha pulegium, Mentha requienii, Mentha rotundi folia, Mentha spicata, Mentha cervina, other species belonging to the Mentha genus.
In particular, for the carrying out of all objects of the invention, essential oil of species belonging to the Mentha genus, more particularly Mentha spicata essential oil could be used.
As mentioned above, according to the present invention, Origanum spp. essential oil denotes essential oil extracted from plants of any species belonging to the Origanum genus, or even a mixture thereof. Among these, for the purposes of the present invention, essential oils of one or more species, or hybrids thereof, selected e.g. from the group comprising Origanum acutidens, Origanum akhdarense, Origanum amanum, Origanum bargyli, Origanum bilgeri, Origanum boissieri, Origanum brevidens, Origanum calcaratum, Origanum compactum, Origanum cordifolium, Origanum cyrenaicum, Origanum dayi, Origanum dictamnus, Origanum dubium, Origanum ehrenbergii, Origanum elongatum, Origanum floribundum, Origanum grosii, Origanum haussknechtii, Origanum hypericifolium , Origanum isthmicum, Origanum kopetdaghense, Origanum laevigatum, Origanum leptocladum, Origanum libanoticum, Origanum lirium, Origanum majorana, Origanum majoricum, Origanum microphyllum, Origanum minutiflorum, Origanum munzurense, Origanum onites, Origanum pampaninii, Origanum ramonense, Origanum rotundifolium, Origanum saccatum, Origanum scabrum, Origanum sipyleum, Origanum solymicum, Origanum symes, Origanum syriacum, Origanum tyttanthum, Origanum vetteri, Origanum virens, Origanum vogelii, Origanum vulgare and other species belonging to this genus could be used.
In particular, for the carrying out of all objects of the invention, essential oil of species belonging to Origanum genus, more particularly, Origanum vulgare essential oil could be used.
In one embodiment, the composition according to the present invention comprises as active ingredients,
0.1-2% w/w each, of at least two of
Mentha spp. essential oil
Origanum spp. essential oil and
Eugenia caryophyllata essential oil as defined above, and further comprises 0.5-4% w/w of one or more
phytopharmacologically acceptable fixing agents,
1-20% w/w of one or more phytopharmacologically acceptable emulsifiers, or of one or more phytopharmacologically acceptable surfactants or a mixture thereof, and one or more phytopharmacologically acceptable carriers.
Some non-limiting examples of ranges of percent by weight (% w/w) of the active ingredients of the composition according to the invention are represented by:
0.1-1.7% w/w of Mentha spp. essential oil
0.1-2% w/w of Origanum spp. essential oil; or 0.1-1 % w/w of Mentha spp. essential oil
0.1-1.5% w/w of Origanum spp. essential oil; or
0.1-1 % w/w of Mentha spp. essential oil
0.1-1% w/w of Origanum spp. essential oil; or
0.1-0.8% w/w of Mentha spp. essential oil
0.1-0.8% w/w of Origanum spp. essential oil; or
0.1-0.4% w/w of Mentha spp. essential oil
0.1-0.4% w/w of Origanum spp. essential oil, or
0.1-1.7% w/w of Origanum spp. essential oil
0.1-2% w/w of Eugenia caryophyllata essential oil; or
0.1-1 % w/w of Origanum spp. essential oil
0.1-1.5% w/w of Eugenia caryophyllata essential oil; or
0.1-1 % w/w of Origanum spp. essential oil
0.1-1 % w/w of Eugenia caryophyllata essential oil; or
0.1-0.8% w/w of Origanum spp. essential oil
0.1-0.8% w/w of Eugenia caryophyllata essential oil; or
0.1-0.4% w/w of Origanum spp. essential oil
0.1-0.4% w/w of Eugenia caryophyllata essential oil, or 0.1 1.7% w/w of Origanum spp. essential oil
0.1 2% w/w of Eugenia caryophyllata essential oil; or
0.1 1 % w/w of Origanum spp. essential oil
0.1 1.5% w/w of Eugenia caryophyllata essential oil; or
0.1 1 % w/w of Origanum spp. essential oil
0.1 1 % w/w of Eugenia caryophyllata essential oil; or
0.1 0.8% w/w of Origanum spp. essential oil
0.1 0.8% w/w of Eugenia caryophyllata essential oil; or
0.1 0.4% w/w of Origanum spp. essential oil
0.1 0.4% w/w of Eugenia caryophyllata essential oil, or
0.1 1.5% w/w of Mentha spp. essential oil
0.1 1.5% w/w of Eugenia caryophyllata essential oil and 0.1 1.5% w/w of Origanum spp. essential oil; or
0.1 1 % w/w of Mentha spp. essential oil
0.1 1 % w/w of Eugenia caryophyllata essential oil and 0.1 1 % w/w of Origanum spp. essential oil; or
0.1 0.8% w/w of Mentha spp. essential oil
0.1 0.8% w/w of Eugenia caryophyllata essential oil and 0.1 0.8% w/w of Origanum spp. essential oil; or
0.1 0.4% w/w of Mentha spp. essential oil
0, 1 0.4 w/w of Eugenia caryophyllata essential oil and 0.1 0.4% w/w of Origanum spp. essential oil; or 0.1-0.3% w/w of Mentha spp. essential oil
0.1-0.3% w/w of Eugenia caryophyllata essential oil and
0.1-0.3% w/w of Origanum spp. essential oil.
As mentioned above, in a preferred embodiment, Mentha spp. essential oil is Mentha spicata essential oil.
In a further preferred embodiment, Origanum spp. essential oil is Origanum vulgare essential oil.
In a particularly preferred embodiment, Mentha spp. essential oil is Mentha spicata essential oil and Origanum spp. essential oil is Origanum vulgare essential oil.
The phytopharmaceutically acceptable carrier could be any carrier that be not phytotoxic for the plant and that be acceptable for the desired agricultural purposes. The carrier could be oily or aqueous.
According to the invention, the choice of an aqueous-type carrier is preferred, since the data obtained by the Authors of the invention and reported below show a greater plant growth regulating effectiveness of the active ingredients selected when administered with an aqueous carrier, oft-times associated with a lesser phytotoxicity thereof.
Effectiveness and phytotoxicity of some of the combinations falling within the embodiments of the present invention are reported hereinafter.
The modes by which anti-sprouting effectiveness and phytotoxicity assessment were attributed are explained in detail in the experimental section.
Table 1
Anti-sprouting Wks.
Components in % w/w Carrier effectiveness persistence Phytotoxic
2% Origanum spp.+ 2%
Mentha spp. Water high 9/10 4 On average
0.8% Origanum spp.+ 0.8%
Mentha spp. Water high 9/10 4 Weakly
0.6% Origanum spp.+ 0.6%
Mentha spp. Water high 9/10 4 Weakly 0.4% Origanum spp.+ 0.4%
Mentha spp. Water high 9/10 4 Weakly
0.75% Origanum spp.+ 0.9%
Mentha spp. Water high 9/10 4.5 NO
0.35% Origanum spp.+
0.35% Mentha spp. Water high 9/10 4 Weakly
Each of the above-indicated embodiments is suitable for the carrying out of the invention.
By way of example, the composition could be as follows:
2% Origanum spp.+ 2% Mentha spp. + at least one phytopharmacologically acceptable co-formulant
0.8% Origanum spp.+ 0.8% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.6% Origanum spp.+ 0.6% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.4% Origanum spp.+ 0.4% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.75% Origanum spp.+ 0.9% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.35% Origanum spp.+ 0.35% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.5% Origanum spp.+ 0.5% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.45% Origanum spp.+ 0.45% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.35% Origanum spp.+ 0.45% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.45% Origanum spp.+ 0.35% Mentha spp. + at least one phytopharmacologically acceptable co-formulant
2% Origanum spp.+ 2% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-formulant 0.8% Origanum spp.+ 0.8% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-formula 0.6% Origanum spp.+ 0.6% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-formula 0.4% Origanum spp.+ 0.4% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-formula 0.75% Origanum spp.+ 0.9% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-formu 0.35% Origanum spp.+ 0.35% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-form 0.5% Origanum spp.+ 0.5% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-formula 0.45% Origanum spp.+ 0.45% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-form 0.35% Origanum spp.+ 0.45% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-form 0.45% Origanum spp.+ 0.35% Eugenia caryophyllata + at least one phytopharmacologically acceptable co-form
2% Eugenia caryophyllata + 2% Mentha spp. + at least one phytopharmacologically acceptable co-formulant 0.8% Eugenia caryophyllata + 0.8% Mentha spp. + at least one phytopharmacologically acceptable co-formulan- 0.6% Eugenia caryophyllata + 0.6% Mentha spp. + at least one phytopharmacologically acceptable co-formulan- 0.4% Eugenia caryophyllata + 0.4% Mentha spp. + at least one phytopharmacologically acceptable co-formulan-
0.75% Eugenia caryophyllata + 0.9% Mentha spp. + at least one phytopharmacologically acceptable co-formula
0.35% Eugenia caryophyllata + 0.35% Mentha spp. + at least one phytopharmacologically acceptable co-formul
0.5% Eugenia caryophyllata + 0.5% Mentha spp. + at least one phytopharmacologically acceptable co-formulan-
0.45% Eugenia caryophyllata + 0.45% Mentha spp. + at least one phytopharmacologically acceptable co-formul
0.35% Eugenia caryophyllata + 0.45% Mentha spp. + at least one phytopharmacologically acceptable co-formul
0.45% Eugenia caryophyllata + 0.35% Mentha spp. + at least one phytopharmacologically acceptable co-formul
1 % Eugenia caryophyllata +1 % Mentha spp. + 1 % Origanum spp.+ at least one phytopharmacologically acceptable co-formulant
0.8% Eugenia caryophyllata + 0.8% Mentha spp. + 0.8% Origanum spp.+ at least one phytopharmacologically acceptable co-formulant
0.6% Eugenia caryophyllata + 0.6% Mentha spp. + 0.6% Origanum spp. +at least one phytopharmacologically acceptable co-formulant
0.4% Eugenia caryophyllata + 0.4% Mentha spp. + 0.4% Origanum spp. +at least one phytopharmacologically acceptable co-formulant
0.7% Eugenia caryophyllata + 0.7% Mentha spp. + 0.7% Origanum spp. + at least one phytopharmacologically acceptable co-formulant
0.35% Eugenia caryophyllata + 0.35% Mentha spp. + 0.35% Origanum spp. + at least one phytopharmacologici acceptable co-formulant
0.5% Eugenia caryophyllata + 0.5% Mentha spp. + 0.5% Origanum spp. + at least one phytopharmacologically acceptable co-formulant
0.45% Eugenia caryophyllata + 0.45% Mentha spp. + 0.45% Origanum spp. + at least one phytopharmacologici acceptable co-formulant
0.3% Eugenia caryophyllata + 0.4% Mentha spp. + 0.3% Origanum spp. + at least one phytopharmacologically acceptable co-formulant
0.25% Eugenia caryophyllata + 0.25% Mentha spp. + 0.25% Origanum spp. + at least one phytopharmacologici acceptable co-formulant
The term Origanum spp. in the examples and in the tables above could be replaced in any point by any one of the terms Origanum acutidens, Origanum akhdarense, Origanum amanumL Origanum bargyli, Origanum bilgeri, Origanum boissieri, Origanum brevidens, Origanum calcaratum, Origanum compactum, Origanum cordifolium, Origanum cyrenaicum, Origanum dayi, Origanum dictamnus, Origanum dubium, Origanum ehrenbergii, Origanum elongatum, Origanum floribundum, Origanum grosii, Origanum haussknechtii, Origanum hypericifolium, Origanum isthmicum, Origanum kopetdaghense, Origanum laevigatum, Origanum leptocladum, Origanum libanoticum, Origanum lirium, Origanum majorana, Origanum majoricum, Origanum microphyllum, Origanum minutiflorum, Origanum munzurense, Origanum onites, Origanum pampaninii, Origanum ramonense, Origanum rotundifolium, Origanum saccatum, Origanum scabrum, Origanum sipyleum, Origanum solymicum, Origanum symes, Origanum syriacum, Origanum tyttanthum, Origanum vetteri, Origanum virens, Origanum vogelii, Origanum vulgare, whereas the term Mentha spp. could be replaced in any point of the examples and of the tables above by any one of the terms Mentha aquatica, Mentha germanica, Mentha arvensis, Mentha citrata, Mentha bergamot, Mentha gentilis, Mentha aurea, Mentha piperita , which is a hybrid between Mentha aquatica and Mentha viridis, Mentha pulegium, Mentha requienii, Mentha rotundifolia, Mentha spicata, Mentha cervina.
In a particular embodiment, the term Origanum spp. is replaced by the term Origanum vulgare and the term Mentha spp. is replaced by the term Mentha spicata.
The percentages in the tables and in the examples above are always meant as % w/w.
According to the invention, said one or more phytopharmacologically acceptable fixing agent is selected from resins derived from Coniferae, in particular wherein said Coniferae may be species belonging to the Pinus genus.
In one embodiment, said resins comprise or consist of poly-1-p-menthene
According to the invention, said one or more phytopharmacologically acceptable emulsifier could be an emulsifier commonly used in agriculture, and could be selected, in a non-limiting manner, from polyglycerols, sucrose esters emulsifiers, or a mixture thereof.
By way of example, said polyglycerols may be selected from polyglyceryl- 10- caprylate/caprate, polyglyceryl-10-decaoleate, polyglyceryl-3-diisostearate, polyglyceryl polyricinoleate, polyglyceryl 6 diisostearate, polyglyceryl 6 dioleate; and, always by way of example, said sucrose esters are selected from sucrose stearate, sucrose palmitate, sucrose laurate. In one embodiment, one or more of said phytopharmacologically acceptable tackifying agents, fixing agents, surfactants, emulsifiers, carriers are of natural origin.
Hereinafter, some non-limiting examples of the composition according to the invention are provided.
a)
Components % w/w Mentha spicata essential oil 0.8
Origanum vulgare essential oil 0.8
Poly-1 -p-menthene 1
Polyglyceryl-10 caprylate/caprate 3
q.s. to
Water 100 b)
Components % w/w
Mentha spicata essential oil 0.4
Origanum vulgare essential oil 1
Poly-1 -p-menthene 2
Polyglyceryl 10-decaoleate 5
q.s. to
Water 100 c)
Components % w/w
Mentha spicata essential oil 1 .3
Origanum vulgare essential oil 2
Poly-1 -p-menthene 3
Polyglyceryl 3-diisostearate 4
q.s. to
Water 100 d)
Components % w/w
Mentha spicata essential oil 0.1
Origanum vulgare essential oil 1 .7
Pine resin 3
Polyglyceryl 3-diisostearate 4
q.s. to
Water 100 e)
Components % w/w
Mentha spicata essential oil 0.4
Origanum vulgare essential oil 0.8
Poly-1 -p-menthene 3 Sucrose palmitate 4
Sucrose laurate 2
q.s. to
Water 100
Components % w/w
Mentha spicata essential oil 0.1 5
Eugenia caryophyllata essential oil 0.3
Origanum vulgare essential oil 1
Poly-1 -p-menthene 2
Polyglyceryl 10-decaoleate 5
q.s. to
Water 100
g)
Components % w/w
Eugenia caryophyllata essential oil 0.6
Origanum vulgare essential oil 1
Poly-1 -p-menthene 2
Polyglyceryl 10-decaoleate 5
q.s. to
Water 100
h)
Components % w/w
Mentha spicata essential oil 0.1
Eugenia caryophyllata essential oil 1 .9
Poly-1 -p-menthene 3
Sucrose palmitate 4
Sucrose laurate 3
q.s. to
Water 100
According to the present invention, each of the compositions a), b), c), d) or e) may further comprise one or more phytopharmaceutically acceptable tackifying agent and/or fixing agent in order to prolong the presence thereof on the plant and to consequently reduce the number of treatments required. In any of the above-reported embodiments, the term "comprises" can be replaced by the term "be comprised of".
It is understood that in all embodiments exemplified in the present description and claimed, the composition can be read as being comprised of the components listed, rather than "comprise" the components listed.
Object of the invention is also an anti-sprouting (sprouting-suppressing) product comprising the composition as described above in suitable quantities and in suitable vessels. In particular, the product in its packaging could contain all indications required for on-plant use, and all safety indications required, according to the Country in which it will be marketed.
Therefore, the invention also relates to the use of the composition or the anti- sprouting product as described hitherto to inhibit the sprouting of axillary and/or adventitious buds in flowered or topped plants, or to inhibit the growth of young seedlings at the stage between the first and the fourth leaf, and methods to inhibit the sprouting of axillary and/or adventitious buds in the aerial part of herbaceous plants trees or shrubsT comprising one or more treatment steps of treating said plants with a composition or with the anti-sprouting product as previously described and claimed.
According to the present description, the treatment may be carried out by manual administration, by nebulization on said plants, by spraying.
The invention further relates to a process for the preparation of a composition as defined in any one of claims 1 to 15, comprising one or more steps of mixing of at least two of
Mentha spp. essential oil Origanum spp. essential oil, and
Eugenia caryophyllata essential oil, with at least one phytopharmacologically acceptable co-formulating agent,
Wherein said Mentha spp. essential oil
Origanum spp. essential oil and Eugenia caryophyllata essential oil are at a final concentration of 0.1-2% w/w each, optionally by subsequent dilution of a concentrated mother solution.
The mother solution could be prepared at concentrations enabling a convenient dilution (e.g., in water or in an aqueous carrier) like, e.g., 2x, 5x, 10x, 20x, 25x, 30x, 35x, 40x, 45x, 50x, etc. The preparation of a concentrated mother solution enables a convenient storage of the product and a rapid dilution thereof, even directly in-field.
The percentages of active ingredients and of the further components of the composition could be according to any one of the embodiments indicate in the present description. As mentioned above, all applications of the above-described invention are suitable for the treatment of the aerial part of herbaceous plants, shrubs and trees. Such plants comprise, by way of non-exhaustive example, Tobacco, Grape vine, Hazel, Pomegranate, Lime tree and Olive tree. The formulation of the composition in all of the above-described objects could be a micellar-type formulation, of oil-in-water (or oil-in aqueous carrier) emulsion.
Hereinafter some scientific experiments carried out by the Authors of the invention are reported, showing the non-applicability of the state of the art existing on potato to the aerial part of herbaceous plants, shrubs and trees.
The experiments carried out show also the non-applicability of any essential oil or of any concentration to the plant growth regulating treatment to inhibit the sprouting or axillary and/or adventitious buds.
Moreover, the experiments reported below show how the association identified by the Authors of the invention be advantageous in terms of anti-sprouting effectiveness and of phytotoxicity associated thereto.
The optimal formulations reported below, and the most effective administration modes reported below represent an example of particularly effective embodiment of the present invention.
Assays based on the use, at different concentrations, of various types of individual essential oils were carried out, with single-component treatments in two different types of dispersing vehicles: sunflower seed oil and water. Following these treatments, it was possible to verify their plant growth regulating activity as inhibitor of the sprouting of axillary buds, for how long said activity lasted, and above all if there were phenomena of phytotoxicity, localized or spread to leaf tissue, getting as far as plant death.
Observational assays and results are synthesized in the following tables. All assays were conducted by using essential oil of the plants indicated in the various tables, even when the caption e.o. (essential oil) is not used.
The assays conducted in succession, whose results are reported in Table 2, take into account the current state of the art, i.e., reducing essential oil concentration by using sunflower oil as a solvent (inexpensive and solvent for essential oils).
Table 2
Figure imgf000022_0001
As is evident from the data reported above in Table 2, the dilution of the essential oil in an attempt at decreasing phytotoxicity did not reach its goal in the above-reported assays, whereas a proportional decrease of effectiveness was observed (medium and scarce results).
The solvent was then replaced with water.
Table 3
Components in % w/w Carrier Wks. persistence Phytotoxic
Anti-sprouting effectiveness
4% Red thyme e.o. water medium 6/10 2 Weakly
4% Origanum vulgare e.o. water medium 6/10 2 Strongly
Plant
4% Mentha spicata e.o. water medium 6/10 3 Strongly death
2% Red thyme e.o. water nil 2 Weakly
2% Origanum vulgare e.o. water scarce 3/10 2 Weakly
Plant
2% Mentha spicata e.o. water scarce 3/10 3 Strongly death
1 % Red thyme e.o. water nil 2 No
1 % Origanum vulgare e.o. water scarce 3/10 2 No
1 % Mentha spicata e.o. water scarce 3/10 2 Weakly
The results reported above in Table 3 demonstrate that there is no improvement as to effectiveness, and a reduction of on-leaf persistence of the preparation is added which can be directly correlated to phytotoxicity reduction.
Key to symbols for data assessment
Assessment of anti-sprouting effectiveness: considering the
activity on the 10 axillary buds below the topping zone:
0/10 = nil
3/10 = scarce
6/10 = medium
9/10 = high
Evaluation of phytotoxic action, both on leaves and on
the entire plant with lethal outcome:
No = no burn activity of the leaf lamina is noticed
Weakly = A slight burn in zones adjacent to the petiole area and on
the leaf lamina referred thereto is noticed
Strongly = evident burns, in a short time causing loss of leaf turgor, and in some cases plant death.
Weeks of persistence of anti-sprouting activity during which no axillary bud sprouting is shown
The lucky hand of the Inventor carried on the experimentations, finding that the combination of Mentha spicata and Origanum vulgare essential oils in concentrations far lower than those reported in the literature solved the abovecited issues, and also discovering the anti-sprouting activity of Eugenia caryophyllata essential oil, alone or in combination with one or both of the above-mentioned essential oils. In particular, by reducing the concentrations from 4% to the range of 0.1-2, and above all in the range of 0.3-1 , an increase of activity is had, acccompanied by a reduction of phytotoxicity without decrease of on-leaf persistence of the preparation. In table 4 below, the anti-sprouting effectiveness, on-plant duration and phytotoxicity of various of the formulations assayed on Tobacco are reported.
Table 4
Figure imgf000024_0001
In order to be able to assay and verify the synergy of the effectiveness as plant growth regulator inhibiting the sprouting of buds of topped Tobacco plants, successive assays and evaluations were carried out on in-field plants, by using various mixtures with variable contents of essential oils, finally demonstrating what follows:
The percentage of essential oils present in the mixture proposed as plant growth regulator was drastically reduced, stabilizing for Mentha spicata e.o. in a range of 0.1 to 1.7% and for Origanum vulgare e.o. in a final concentration range of from 0.1 to 2%.
The anti-sprouting activity was highlighted in-field and caused blocking of axillary buds, at first with a real caustic action aimed at apexes present, and secondarily with a retarding effect that actually blocked for some weeks the growth (sprouting) of buds that anyhow the plant attempts to produce in response to elimination of apical dominance.
At the suggested doses, there were observed no phytotoxic effects such as those evident in the use of Mentha spicata oil alone, at much higher dosages. The applying was carried out with manual administration on Black Tobacco, by pouring the expected amount on the apical portion of the stem, and by nebulization on White Tobacco, by sprinkling over the foliage from above with automated machines.
Anti-sprouting activity on tomato plants of the following combinations, reported in the Table, was also assayed
Figure imgf000025_0001
Instead, the treatment with sole Origanum v. essential oil = 0.75% w/w proved devastating, in the sense that it exhibits a very high degree of phytotoxicity, destroying all plants contacted with the solution (burns, dehydration, scorching, stem necrosis, etc.) and also the treatment with sole Eugenia caryophyllata essential oil = 0.75% w/w highlighted a remarkable phytotoxicity (slightly below Origanum) with partial destruction of the aerial part; also the treatment with alike percentages of Mentha spicata essential oil proved anyhow to have phytotoxicity.
It is interesting to note that, when combined, the essential oils of the invention are found to keep a strong anti-sprouting power, with a reduction of the phytotoxic power thereof.

Claims

1. A composition comprising 0.1-2% w/w each, of at least two of Mentha spp. essential oil
Origanum spp. essential oil and
Eugenia caryophyllata essential oil, and at least one phytopharmacologically acceptable co-formulating agent.
2. The composition according to claim 1 , comprising
0.1-2% w/w of Mentha spp. essential oil
0.1-2% w/w of Origanum spp. essential oil, or
0.1-2% w/w of Mentha spp. essential oil
0.1-2% w/w of Eugenia caryophyllata essential oil, or
0.1-2% w/w of Origanum spp. essential oil, and
0.1-2% w/w of Eugenia caryophyllata essential oil, or
0.1-1 % w/w of Mentha spp. essential oil
0.1-1 % w/w of Origanum spp. essential oil
0.1-1 % w/w of Eugenia caryophyllata essential oil and at least one phytopharmacologically acceptable co-formulating agent. 3. The composition according to claim 2, comprising
0.1-1.7% w/w of Mentha spp. essential oil
0.1-2% w/w of Origanum spp. essential oil; or
0.1-1 % w/w of Mentha spp. essential oil
0.1-1.5% w/w of Origanum spp. essential oil; or
0.1-1 % w/w of Mentha spp. essential oil
0.1-1 % w/w of Origanum spp. essential oil; or 0.1-0.8% w/w of Mentha spp. essential oil
0.1-0.8% w/w of Origanum spp. essential oil; or
0.1 -0.4% w/w of Mentha spp. essential oil
0.1-0.4% w/w of Origanum spp. essential oil, or
0.1-1.7% w/w of Origanum spp. essential oil
0.1-2% w/w of Eugenia caryophyllata essential oil; or
0.1-1 % w/w of Origanum spp. essential oil
0.1-1.5% w/w of Eugenia caryophyllata essential oil; or
0.1-1 % w/w of Origanum spp. essential oil
0.1-1 % w/w of Eugenia caryophyllata essential oil; or
0.1-0.8% w/w of Origanum spp. essential oil
0.1-0.8% w/w of Eugenia caryophyllata essential oil; or 0.1-0.4% w/w of Origanum spp. essential oil
0.1-0.4% w/w of Eugenia caryophyllata essential oil, or
0.1-1.7% w/w of Origanum spp. essential oil
0.1-2% w/w of Eugenia caryophyllata essential oil; or
0.1-1 % w/w of Origanum spp. essential oil
0.1-1.5% w/w of Eugenia caryophyllata essential oil; or
0.1-1 % w/w of Origanum spp. essential oil
0.1-1 % w/w of Eugenia caryophyllata essential oil; or
0.1-0.8% w/w of Origanum spp. essential oil
0.1-0.8% w/w of Eugenia caryophyllata essential oil; or 0.1-0.4% w/w of Origanum spp. essential oil
0.1-0.4% w/w of Eugenia caryophyllata essential oil; or
0.1-1.5% w/w of Mentha spp. essential oil
0.1-1.5% w/w of Eugenia caryophyllata essential oil and
0.1-1.5% w/w of Origanum spp. essential oil; or 0.1-1 % w/w of Mentha spp. essential oil
0.1-1 % w/w of Eugenia caryophyllata essential oil, and
0.1-1 % w/w of Origanum spp. essential oil; or
0.1-0.8% w/w of Mentha spp. essential oil
0.1-0.8% w/w of Eugenia caryophyllata essential oil, and
0.1-0.8% w/w of Origanum spp. essential oil; or
0.1-0.4% w/w of Mentha spp. essential oil
0, 1-0.4 w/w of Eugenia caryophyllata essential oil and
0.1-0.4% w/w of Origanum spp. essential oil; or
0.1-0.3% w/w of Mentha spp. essential oil
0.1-0.3% w/w of Eugenia caryophyllata essential oil and
0.1-0.
3% w/w of Origanum spp. essential oil and at least one
phytopharmacologically acceptable co-formulating agent.
4. The composition according to any one of claims 1 to 3, wherein said at least one phytopharmacologically acceptable co-formulating agent is a tackifying agent, a fixing agent, a surfactant, an emulsifier, a carrier.
5. The composition according to any one of claims 1 to 4, wherein said at least one phytopharmacologically acceptable co-formulating agent comprises 0.5-4% w/w of one or more phytopharmacologically acceptable fixing agents, 1-20% w/w of one or more phytopharmacologically acceptable emulsifiers, or of one or more phytopharmacologically acceptable surfactants or a mixture thereof, and one or more phytopharmacologically acceptable carriers.
6. The composition according to any one of claims 1 to 5, wherein said Mentha spp. is selected from Mentha aquatica, Mentha germanica, Mentha arvensis, Mentha citrata, Mentha bergamot, Mentha gentilis, Mentha aurea, Mentha piperita, which is a hybrid between Mentha aquatica and Mentha viridis, Mentha pulegium, Mentha requienii, Mentha rotundifolia, Mentha spicata, Mentha cervina.
7. The composition according to any one of claims 1 to 6, wherein said Origanum spp. is selected from Origanum acutidens, Origanum akhdarense, Origanum amanum, Origanum bargyli, Origanum bilgeri, Origanum boissieri, Origanum brevidens, Origanum calcaratum, Origanum compactum, Origanum cordifolium, Origanum cyrenaicum, Origanum dayi, Origanum dictamnus, Origanum dubium, Origanum ehrenbergii, Origanum elongatum, Origanum floribundum, Origanum grosii, Origanum haussknechtii, Origanum hypericifolium, Origanum isthmicum, Origanum kopetdaghense, Origanum laevigatum, Origanum leptocladum, Origanum libanoticum, Origanum lirium, Origanum majorana, Origanum majoricum, Origanum microphyllum, Origanum minutiflorum, Origanum munzurense, Origanum onites, Origanum pampaninii, Origanum ramonense, Origanum rotundifolium, Origanum saccatum, Origanum scabrum, Origanum sipyleum, Origanum solymicum, Origanum symes, Origanum syriacum, Origanum tyttanthum, Origanum vetteri, Origanum virens, Origanum vogelii, Origanum vulgare.
8. The composition according to any one of claims 1 to 7, wherein said Mentha spp. is Mentha spicata and said Origanum spp. is Origanum vulgare.
9. The composition according to any one of claims 1 to 8, wherein said carrier is an aqueous carrier.
10. The composition according to any one of claims 1 to 9, wherein said one or more phytopharmacologically acceptable fixing agent is selected among resins derived from Coniferae.
1 1. The composition according to claim 10, wherein said Coniferae are species from the Pinus genus.
12. The composition according to claim 11 , wherein said resins comprise or consist of poly-1-p-menthene.
13. The composition according to any one of claims 1 to 12, wherein said one or more phytopharmacologically acceptable emulsifier is selected from polyglycerols, sucrose esters.
14. The composition according to any one of claims 1 to 13, wherein one or more of said one or more among phytopharmacologically acceptable tackifying agents, fixing agents, surfactants, emulsifiers, carriers or wetting agents are of natural origin.
15. The composition according to any one of claims 1 to 14, in a 2x, 5x, 10x, 20x, 25x, 30x, 35x, 40x, 45x or 50x concentrated form.
16. An anti-sprouting (sprouting-suppressing) product comprising the composition according to any one of claims 1 to 15 in suitable quantities and in suitable vessels.
17 Use of a combination of at least two of
Mentha spp. essential oil
Origanum spp. essential oil and
Eugenia caryophyllata essential oil
to inhibit the sprouting of axillary and/or adventitious buds in flowered or topped plants, or to inhibit the growth of young seedlings at the stage between the first and the fourth leaf.
18. The use of the composition according to any one of claims 1 to 14 or of the anti-sprouting product according to claim 16, suitably diluted if needed, to inhibit the sprouting of axillary and/or adventitious buds in flowered or topped plants or to inhibit the growth of young seedlings at the stage between the first and the fourth leaf.
19. A method to inhibit the sprouting of axillary and/or adventitious buds in herbaceous plants, shrubs and/or treesT comprising one or more treatment steps of treating said plants with a composition as defined in claims 1 to 14, or with the anti- sprouting product according to claim 16, suitably diluted if needed.
20. The method according to claim 19, wherein said treatment is carried out by means of manual administration, by nebulization or by spraying of said composition or product on said plants.
21. A process for the preparation of a composition as defined in any one of claims 1 to 14, comprising one or more steps wherein at least two of
Mentha spp. essential oil
Origanum spp. essential oil and
Eugenia caryophyllata essential oil are admixed with at least one
phytopharmacologically acceptable co-formulating agent, wherein said at least two of Mentha spp. essential oil
Origanum spp. essential oil and
Eugenia caryophyllata essential oil
are at a final concentration of 0.1-2% w/w each, said process optionally comprising a step of dilution of a concentrated form of said composition as defined in claim 15.
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Publication number Priority date Publication date Assignee Title
CN114794104A (en) * 2022-04-12 2022-07-29 山东农业工程学院 Plant growth regulator, preparation method and use method
US11771095B2 (en) 2020-03-13 2023-10-03 Harpe Bioherbicide Solutions Inc. Herbicidal Mentha pantsd, extract compositions and methods of using same000000000000000,001

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CN114794104B (en) * 2022-04-12 2024-04-02 山东农业工程学院 Plant growth regulator, preparation method and use method

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