WO2018102345A1 - Utilisation de l'auréobasidine antifongique en agriculture - Google Patents
Utilisation de l'auréobasidine antifongique en agriculture Download PDFInfo
- Publication number
- WO2018102345A1 WO2018102345A1 PCT/US2017/063599 US2017063599W WO2018102345A1 WO 2018102345 A1 WO2018102345 A1 WO 2018102345A1 US 2017063599 W US2017063599 W US 2017063599W WO 2018102345 A1 WO2018102345 A1 WO 2018102345A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- infection
- agricultural product
- aureobasidin
- composition according
- agricultural
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
Definitions
- the present invention is concerned with the use of the natural product aureobasidin A (AbA) as an agricultural fungicide.
- the invention is also concerned with compositions comprising aureobasidin A (AbA), their preparation, and methods of using such compositions to control agricultural pests including, but not limited to, fungi selected from the group consisting of Magnaporthe oryzae, Alternaria solani, Septoria triticum, Fusarium oxysporum, and Rhizoctonia solani.
- Aureobasidin A-R are antifungal cyclic depsipeptide antibiotics produced by Aureobasidium pullulans (Takesako et al, Journal of Antibiotics, 1991, 44, 919-924).
- Aureobasidin A is the most prominent, potent and studied member of this class of depsipeptides (Takesako et al, Journal of Antibiotics, 1991, 44, 919-924; Ikai et al, Journal of Antibiotics, 1991, 44, 925-933; and Takesako et al, Journal of Antibiotics, 1993, 46, 1414-1420). It was shown to have excellent activity against Candida, Aspergillus and Cryptococcus (U.S. Patent No. 5,698,670; Takesako et al, Journal of Antibiotics, 1993, 46, 1414-1420).
- AbA targets the inositol phophorylceramide (IPC) synthase, a key enzyme catalyzing sphingolipid synthesis in fungi (Cerantola et al., Molecular Microbiology 2009, 71, 1523-1537) but which is absent in mammals.
- IPC inositol phophorylceramide
- Magnaporthe oryzae is a hemibiotrophic fungal pathogen that causes rice (Oryza sativa) blast, the most damaging disease on rice.
- Alternaria solani is a fungal pathogen that produces a disease in tomato and potato plants called early blight that is extremely destructive.
- Septoria tritici blotch (STB) caused by the ascomycete fungus Mycosphaerella graminicola (asexual stage: Septoria tritici), is one of the most important foliar diseases of wheat. Fusarium oxysporum is a pathogenic fungus common in soils around the world, and the cause of fusarium wilt, a deadly vascular wilting syndrome in plants.
- Fusarium oxysporum comprises over 120 known strains or "special forms" (formae speciales; f. sp.), each of which is specific to a unique plant host in which it causes disease.
- special forms formae speciales; f. sp.
- These F. oxysporum strains infect and kill a large host range including many commercially harvested crops such as species in the Solenaceae family (tomatoes, peppers, potatoes, eggplant), watermelon, lettuce, legumes, beets, basil, strawberries, chrysanthemum, sugarcane, bananas, and multiple other species.
- Rhizoctonia solani causes a wide range of commercially significant plant diseases.
- Brown patch a turfgrass disease
- damping off e.g., in soybean seedlings
- black scurf of potatoes bare patch of cereals
- root rot of sugar beets a turfgrass disease
- belly rot of cucumber a turfgrass disease
- sheath blight of rice a turfgrass disease
- aureobasidin A is useful as an agricultural fungicide to treat, prevent, or control fungal infections in agricultural products, such as plants and seeds. Plants will include fruits and vegetables.
- aureobasidin A has been found to be effective in treating, preventing, or controlling infections in agricultural products caused by the following fungi: Magnaporthe oryzae, Alternaria solani, Septoria triticum, Fusarium
- a second aspect of the present invention is concerned with agricultural compositions of aureobasidin A comprising an effective amount of the antifungal compound and an agriculturally acceptable carrier.
- Such compositions may additionally comprise one or more excipients selected from the group consisting of (a) one or more diluents, (b) one or more binders or binding agents, (c) one or more dispersing agents, (d) one or more emulsifying agents, (e) one or more surfactants or wetting agents, (f) one or more sticking agents, (g) one or more thickening agents, and (h) a pH adjuster.
- the surfactant is an anionic surfactant.
- the surfactant is a non-ionic surfactant.
- Aureobasidin A and agricultural compositions of aureobasidin A of the present invention may also be used in combination with one or more other agents useful to treat, prevent, or control agricultural pests in the field.
- the compositions of the present invention and other active agents may be administered separately or in conjunction.
- the administration of one element may be prior to, concurrent to, or subsequent to the
- compositions of the present invention examples include, but are not limited to:
- antifungal agents such as Ilicicolin H, azoxystrobin and myclobutanil;
- plant growth regulators such as dikegulac-sodium and trinexapac-ethyl
- herbicides such as prodiamine and glyphosate
- insecticides such as bifenthrin and malathion
- acaricides such as avermectin and kelthane.
- the agricultural compositions of the present invention may contain about 0.1 to 95 percent by weight of aureobasidin A.
- compositions of aureobasidin A may be applied using a variety of methodologies, including soil drench, seed treatment, granular and foliar spray. With regard to the treatment of seeds, the compositions may alternatively be applied directly to the seed before planting of the seed or applied to the locus or soil in which the seeds are sown prior to the planting of the seed.
- the concentration of aureobasidin A to be applied to the agricultural product is about 0.001 to 1 percent by weight. In a class of this method the concentration of aureobasidin A to be applied to the agricultural product is about 0.001 to 0.01 percent by weight.
- Another aspect of the present invention relates to the total amount of aureobasidin A to be applied per area of the field.
- about 2 to about 1000 grams of aureobasidin A are to be applied per acre.
- about 5 to 500 grams of aureobasidin A are to be applied per acre.
- about 0.025 kilograms to about 5 kilograms of aureobasidin A are to be applied per hectare.
- about 0.05 kilograms to about 1 kilogram of aureobasidin A are to be applied per hectare.
- Another aspect of the present invention relates to the use of aureobasidin A and the compositions of the present invention to treat, control, or prevent fungal infections on an agricultural product.
- the present invention also relates to a food, feed, or agricultural product treated with a composition of the present invention.
- the present invention also relates to a process for the treatment of an agricultural product which comprises applying a composition of the present invention to such agricultural product or plant.
- the present invention also relates to the use of a fermentation broth derived from strains of Aureobasidium pullulans as a live cell or cell suspension to treat, control, or prevent a fungal infection on an agricultural product.
- Such fermentation broths contain the antifungal agent aureobasidin A, which need not be isolated, purified, and re-formulated for use as an agricultural fungicide.
- the present invention is concerned with the use of the naturally occurring antifungal aureobasisin A (AbA) to control the growth of agricultural pests including, but not limited to, Magnaporthe oryzae, Alternaria solani, Septoria triticum, Fusarium Oxysporum, and Rhizoctonia solani.
- ABSA antifungal aureobasisin A
- Aureobasidin A can be used to control a variety of plant diseases and can be applied in a variety of methodologies, including soil drench, seed treatment, granular and foliar spray. It can be readily formulated into a variety of compositions, including, but not limited to, emulsifiable concentrates, inverse emulsions, microemulsions, dispersible granules, wettable powders, dusts, and granules.
- Aureobasidin A offers several advantages over existing synthetic fungicides. Since it is derived from natural sources, it already occurs in the environment and biological processes to degrade the compound are already present in the environment. Unlike many conventional fungicides, aureobasidin A does not contain any heavy metals or halogens and therefore is totally biologically degradable. Moreover, aureobasidin A has a different mechanism of action from existing commercial fungicides and therefore can be used to reduce fungicide resistance found in certain plant diseases.
- agricultural product as used herein is to be understood in a very broad sense and includes, but is not limited to, cereals, e.g., wheat, barley, rye, oats, rice, sorghum, and the like; beets, e.g. sugar beet and fodder beet; pome and stone fruit and berries, e.g. apples, pears, plums, apricots, peaches, almonds, cherries, strawberries, raspberries and blackberries; leguminous plants, e.g. beans, lentils, peas, and soybeans; oleaginous plants, e.g.
- rape mustard, poppy, olive, sunflower, coconut, castor-oil plant, cocoa, and ground-nuts
- cucurbitaceae e.g. pumpkins, gherkins, melons, cucumbers, squashes, and aubergines
- fibrous plants e.g. cotton, flax, hemp, and jute
- citrus fruit e.g. oranges, lemons, grapefruits, mandarins, and limes
- tropical fruit e.g. papayas, passion fruit, mangos, carambolas, pineapples, bananas, and kiwis
- vegetables e.g. spinach, lettuce, asparagus
- brassicaceae such as cabbage and turnips, carrots, onions, tomatoes, potatoes, seed potatoes, hot and sweet peppers
- laurel-like plants e.g. avocado, cinnamon, camphor tree
- products such as maize, tobacco, nuts, coffee, sugarcane, tea, grapevines, hops, rubber plants, as well as ornamental plants, e.g.
- cut flowers roses, tulips, lilies, Narcissus, crocuses, hyacinths, dahlias, Gerbia, carnations, fuchsias, chrysanthemums, and flower bulbs, shrubs, deciduous trees and evergreen trees such as conifers, plants and trees in greenhouses. It includes, but is not limited to, plants and their parts, fruits, seeds, cuttings, cultivars, grafts, bulbs, tubers, root-tubers, rootstocks, cut flowers and vegetables.
- Aureobasidium pullulans is grown and cultivated as described in Takesako et al., "Aureobasidins, New antifungal antibiotics taxonomy, fermentation, isolation and properties" Journal of Antibiotics 1991, 44, 919-924.
- Aureobasidium pullulans is inoculated in 100 mL of liquid medium composed of yeast nitrogen base (Difco) 0.67% and glucose (2%) in a 500 mL Erlenmeyer flask. The flask is shaken at 25°C for two days to give a seed culture.
- One-liter seed culture is transferred into 200-liter fermenter containing 100 liters of medium consisting of glucose 2%, ammonium sulfate 0.5%, potassium hydrogen phosphate sodium chloride 0.01%, microgram/mL, and microgram/mL. Twenty liters of a medium containing glucose 10%, ammonium sulfate 2.5%, polypepton 5%, potassium hydrogen phosphate sodium chloride
- microgram/mL, and microgram/mL is supplemented to the culture.
- the fermentation is further continued at 25°C for 78 hours with 120-liters/minute aeration and 100 rpm agitation.
- the fermentation broth from producer strains can be processed in several different ways depending on the utility. In one method, the mycelia are separated and extracted by ethanol, methanol, acetone or ethyl acetate. The extract is concentrated and aureobasidin A is purified by standard chromatographic methods as described by Takesako et al, Journal of Antibiotics, 1991, 44, 919-924. In a second method, the fermentation broth is lyophilized to remove water to afford a dry powder.
- the cells in the fermentation broth are broken if necessary by addition of organic solvent, such as methanol, ethanol, acetone, and the like, and then evaporated to dryness to afford a powder.
- organic solvent such as methanol, ethanol, acetone, and the like
- the fermentation broth obtained directly or after breaking the cells with solvent is sprayed onto the plant by foliar treatment, mixed with seeds, or used as a soil inoculant.
- broth powders generated using either of the two methods is mixed with appropriate agent(s) and sprayed by foliar treatment, mixed with seeds, or used as a soil inoculant.
Abstract
La présente invention concerne l'utilisation du produit naturel de l'auréobasidine A (AbA) en tant que fongicide agricole, des compositions comprenant de l'auréobasidine A, et l'utilisation de telles compositions pour traiter, contrôler ou prévenir une infection fongique dans des produits agricoles, en particulier, les champignons étant choisis dans le groupe constitué de Magnaporthe oryzae, Alternaria
solani, Septoria triticum, Fusarium oxysporum, et Rhizoctonia solani.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662427855P | 2016-11-30 | 2016-11-30 | |
US62/427,855 | 2016-11-30 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2018102345A1 true WO2018102345A1 (fr) | 2018-06-07 |
Family
ID=62241954
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US2017/063599 WO2018102345A1 (fr) | 2016-11-30 | 2017-11-29 | Utilisation de l'auréobasidine antifongique en agriculture |
Country Status (1)
Country | Link |
---|---|
WO (1) | WO2018102345A1 (fr) |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110183521A (zh) * | 2019-05-23 | 2019-08-30 | 华南农业大学 | 稻瘟菌基因MoRMD1在调控稻瘟菌致病力中的应用 |
WO2021245100A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245104A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245105A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245103A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245102A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245106A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2023089007A1 (fr) | 2021-11-19 | 2023-05-25 | Syngenta Crop Protection Ag | Compositions fongicides |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0500264A1 (fr) * | 1991-02-19 | 1992-08-26 | Takara Shuzo Co. Ltd. | Fongicides agricoles et horticoles |
EP0670166A2 (fr) * | 1994-03-01 | 1995-09-06 | Eli Lilly And Company | Compositions pharmaceutiques administrables par voie buccale |
US20140024578A1 (en) * | 2011-04-01 | 2014-01-23 | AureoGen Biosciences ,Inc. | Aureobasidin derivatives and methods of synthesis |
-
2017
- 2017-11-29 WO PCT/US2017/063599 patent/WO2018102345A1/fr active Application Filing
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0500264A1 (fr) * | 1991-02-19 | 1992-08-26 | Takara Shuzo Co. Ltd. | Fongicides agricoles et horticoles |
EP0670166A2 (fr) * | 1994-03-01 | 1995-09-06 | Eli Lilly And Company | Compositions pharmaceutiques administrables par voie buccale |
US20140024578A1 (en) * | 2011-04-01 | 2014-01-23 | AureoGen Biosciences ,Inc. | Aureobasidin derivatives and methods of synthesis |
Non-Patent Citations (1)
Title |
---|
LIU ET AL.: "In vitro inhibition of postharvest pathogens of fruit and control of gray mold of strawberry and green mold of citrus by aureobasidin A.", INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, vol. 119, no. 3, November 2007 (2007-11-01), pages 223 - 229, XP022336528 * |
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110183521A (zh) * | 2019-05-23 | 2019-08-30 | 华南农业大学 | 稻瘟菌基因MoRMD1在调控稻瘟菌致病力中的应用 |
WO2021245100A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245104A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245105A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245103A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245102A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2021245106A1 (fr) | 2020-06-04 | 2021-12-09 | Syngenta Crop Protection Ag | Compositions fongicides |
WO2023089007A1 (fr) | 2021-11-19 | 2023-05-25 | Syngenta Crop Protection Ag | Compositions fongicides |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
WO2018102345A1 (fr) | Utilisation de l'auréobasidine antifongique en agriculture | |
US11286458B2 (en) | Methods and compositions for the biological control of plant pathogens | |
JP6758296B2 (ja) | 植物の成長に利益をもたらす及び植物病害を治療するための微生物組成物 | |
UA120913C2 (uk) | Фунгіцидні композиції | |
TW201637570A (zh) | 地衣芽孢桿菌rti184組合物及益於植物生長之使用方法 | |
MX2012006008A (es) | Mezclas de plaguicidas. | |
CN105638701B (zh) | 一种杀线虫组合物及其用途 | |
WO2015192717A1 (fr) | Composition insecticide synergique comprenant des insecticides néonicotinoïdes et des insecticides pyréthroïdes | |
KR100754053B1 (ko) | 식물의 병해 내성-유도 조성물 및 그의 제조방법 | |
CN109310093A (zh) | 游霉素组合物及其用途 | |
US20190335751A1 (en) | Use of the Antifungal Ilicicolin H in Agriculture | |
AU2015271898B2 (en) | A synergistic composition comprising insecticides and fungicides | |
EP3003047B1 (fr) | Agriculture microbienne | |
KR20110110769A (ko) | 식물 병해 방제용 조성물, 식물 병해 방제 방법, 및 신규 미생물 | |
HU204673B (en) | Fungicidal compositions, process for producing their active ingredient, process for producing veterinary compositions and process for destroying fungi | |
Washington et al. | Dimethomorph soil and seed treatment of potted tomatoes for control of damping-off and root rot caused by Phytophthora nicotianae var. nicotianae | |
TW201709823A (zh) | 地衣芽孢桿菌rti184組合物及益於植物生長之使用方法 | |
WO2018118768A1 (fr) | Utilisation d'acides zaragoziques en tant qu'antifongiques en agriculture | |
CN107593710B (zh) | 一种杀菌组合物 | |
RU2609404C2 (ru) | Способ регулирования роста растений и применение кломазона для улучшения роста растений | |
US20230232836A1 (en) | Mannosylerythritol lipid biological pesticides and applications thereof | |
CN107637601B (zh) | 一种杀菌组合物 | |
CN107593711B (zh) | 一种杀菌组合物 | |
KR20230012463A (ko) | 진균 병원체를 억제하기 위한 조성물 및 방법 | |
KR20240032888A (ko) | 살균제 용도 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 17877016 Country of ref document: EP Kind code of ref document: A1 |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
122 | Ep: pct application non-entry in european phase |
Ref document number: 17877016 Country of ref document: EP Kind code of ref document: A1 |