WO2018090276A1 - Chinese medicine composition for prevention and treatment of combined allergic rhinitis and asthma syndrome and preparation method thereof - Google Patents

Abstract

A Chinese medicine composition for the prevention and treatment of a combined allergic rhinitis and asthma syndrome. The Chinese medicine composition comprises the following components in parts by weight: 16-20 parts of wild chrysanthemum, 9-16 parts of asarum, 16-22 parts of Japanese honeysuckle, 9-14 parts of astragalus membranaceus, 12-16 parts of pinellia ternate, 7-13 parts of sophora flavescens, 8-11 parts of bitter almond, 7-11 parts of lily magnolia, 9-17 parts of licorice, 11-18 parts of uncaria, 11-20 parts of angelica dahurica, 11-16 parts of artemisia argyi, 6-14 parts of pycnostelma, 14-22 parts of divaricate saposhnikovia root, and 10-20 parts of propolis. The Chinese medicine composition is suitable for various populations in the prevention and treatment of respiratory allergic diseases, especially combined allergic rhinitis and asthma syndrome. A method for preparing the Chinese medicine composition is also provided.

Description

Traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome and preparation method thereof Technical field

The invention relates to a traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome and a preparation method thereof, and belongs to the technical field of clinical medicine of otolaryngology.

Background technique

Combined Allergic Rhinitis and Asthma Syndrome (CARAS) is a new medical diagnosis name proposed in recent years, which refers to simultaneous clinical or subclinical upper respiratory tract allergy (allergic rhinitis) and lower respiratory tract. Allergic symptoms (asthma), both often coexist. At present, anti-histamines, anti-allergic drugs or inhaled corticosteroids are the main products on the market. There are certain limitations, and there are no clear clinical studies to prove that Western medicine preparations for suppressing allergic reactions in pregnant women and infants. The desensitization method is a technique according to which allergy may be more or less inhibited by administering a very small amount of an allergen. Since the beginning of the 21st century, WHO has recognized that desensitization is an effective way to cure allergies. In these methods, the patient receives a subcutaneous injection of a dose-increasing natural allergen extract. However, this method can bring about an allergic reaction or even a risk of anaphylactic shock.

technical problem

The object of the present invention is to provide a traditional Chinese medicine composition and an application method having an anti-allergic or desensitizing effect in order to overcome the disadvantages of the existing anti-allergic or desensitizing drugs. The invention resides in the method of absorbing the nasal mucosa through the skin or respiratory mucosa, and can enhance the tolerance to allergens and desensitization of traditional Chinese medicine components, can enhance the anti-allergic ability of the body, and overcome the shortcomings of the existing anti-allergy treatment methods. The composition is safe, has no adverse consequences after long-term use, and does not have the problem of interaction between the drug and the drug, can reduce the toxicity and side effects of the commonly used anti-allergic western medicine, and reduce the dosage or the patient. Take antihistamines and hormones.

Technical solution

In order to solve the above technical problems, the present invention provides a traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome, which is composed of the following parts by weight: wild chrysanthemum 16-20, Asarum 9-16, honeysuckle 16～22, Astragalus 9～14, Pinellia 12～16, Sophora flavescens 7～13, Bitter almond 8～11, Xinyi 7～11, Licorice 9～17, Uncaria 11～18, White peony 11～20, Aiye 11 ~16, Xu Changqing 6 to 14, windproof 14 to 22 parts, propolis 10 to 20.

Preferably, the weight fraction of each component of the traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome is: wild chrysanthemum 20, asarum 14, honeysuckle 20, astragalus 14, pinellia 14, matrine 12, Bitter almond 10, Xinyi 9, licorice 11, hook vine 17, white peony 14, Ai Ye 16, Xu Changqing 13, wind 19, propolis 18.

The composition of the present invention is prevented and treated by the volatilized gas through the human respiratory action in the nasal cavity. The effect of allergic graduation asthma syndrome, its components have the following mechanism of action and its theoretical basis:

Wild chrysanthemum: Wild chrysanthemum contains various active ingredients such as chrysanthemum, wild chrysanthemum, wild chrysanthemum lactone, amino acids and trace elements. Its water extract has obvious protective effect on the cardiovascular system, can increase cardiac output, increase myocardial oxygen supply, and protect the normal physiological function of ischemic myocardium. The wild blue chrysanthemum water extract and the blue-green volatile oil extracted by steam distillation have killing or inhibiting activity against various pathogenic bacteria and viruses, and have anti-inflammatory, anti-oxidative and analgesic activities. The anti-allergic effect of wild chrysanthemum is enhanced by immunity, also known as immune regulation, which regulates the abnormal immunity of the human body (too high and too low) to regulate the normal immune level, thereby enhancing the skin's resistance and enhancing the anti-allergic function. Overcome the side effects of corticosteroids. That is to say, the anti-allergic effect of corticosteroids is an allergic immunosuppressive remedy, and the wild chrysanthemum is an immune-enhancing prevention before allergies.

Asarum: returning to the lungs, kidneys; phlegm and pain, dispelling cold, dissolving cold, warming the lungs, drinking sputum, dispelling snoring, warming the lungs, warming the lungs, having antipyretic effects and inhibiting cellular immunity The effect can significantly inhibit the formation of mouse hemolysin antibody. Honeysuckle: It has been praised as a good medicine for clearing away heat and detoxification. Honeysuckle contains pharmacologically active ingredients such as chlorogenic acid and luteolin. It has various pathogenic bacteria such as hemolytic streptococcus and staphylococcus aureus and pathogenic viruses of upper respiratory tract infection. Strong inhibition, in addition to enhance immunity, liver protection, anti-tumor, anti-inflammatory, antipyretic, etc., its clinical use is very extensive, can also inhibit and kill the pathogenic bacteria in the throat, anti-infective effect on the elderly and children .

Huang Wei: Modern medical research shows that Astragalus has enhanced immune function, liver protection, diuresis, anti-aging, anti-stress, antihypertensive and broader antibacterial effects. It can eliminate experimental nephritis proteinuria, enhance myocardial contractility, and regulate blood sugar levels. Astragalus can not only expand the coronary arteries, improve myocardial blood supply, improve immune function, but also delay the process of cell aging. Pinellia: dampness and phlegm, lowering the vomiting, eliminating phlegm and dispersing. Treatment of wet and cold drinks, vomiting, nausea, cough and phlegm, chest fullness, headache, dizziness and sleeplessness. Exogenous swelling. It is used for phlegm and cough, dizziness, dizziness, vomiting, vomiting, vomiting, chest suffocation, plum nucleus; Pinellia is used to reduce vomiting, dampness and phlegm: it is used to clear the dampness and coldness.

Sophora flavescens: phlegm, asthma, anti-allergic, immunosuppressive effects, mainly through the excitatory beta receptors, especially the β-receptors of the central nervous system, relieve bronchospasm and inhibit the release of antibodies and slow-reacting substances to produce antiasthmatic effects of. The anti-asthmatic effect of alkaloid Ach is stronger than that of aminophylline, and its antiasthmatic effect may act by exciting the β-receptor of the midbrain. Matrine can reduce the release of allergic mediators. It is an immunosuppressive agent, matrine and oxymatrine have obvious inhibitory effects on passive or active skin allergic reactions, and have obvious inhibitory effects on rabbit serum IgE antibody formation.

Bitter almond: antihistamine, antitussive, antiasthmatic effect, bitter almond contains amygdalin, amygdalin can be hydrolyzed by intestinal microbial enzyme or bitter almond in the body to produce traces of hydrocyanide Acid and benzaldehyde have an inhibitory effect on the respiratory center and achieve antitussive and antiasthmatic effects. Responding to cough and phlegm and relieving asthma, the main symptoms of the lung system are coughing, phlegm and wheezing, and have strong pertinence. Therefore, ancient and modern doctors use it as a pulmonary medicine, regardless of internal injuries, new diseases, dysentery, and involving the lungs. More use. Almond water extract can reduce the sensitivity of organs to ammonia stimuli against histamine, acetylcholine, chlorination The excitatory effect of sputum on the smooth muscle of the trachea has obvious antitussive effect. Bitter almond can promote lung respiratory function by promoting the synthesis of pulmonary surfactant (PS). Bitter almond can not only promote the synthesis of PS, but also improve various physiological and biochemical indicators related to lung (such as lung homogenate and lung water volume). , total phospholipids in bronchial lavage and pathological sections, etc.).

Xinyi: scattered cold, through the nose. For cold headache, nasal congestion, nasal congestion, nasal turbidity, lung opening in the nose, Xinyi has astringent and protective effects on the nasal mucosa, reducing secretions, local microvascular dilatation, improved circulation, can promote secretion absorption and inflammation subsided The topical application of Xinyi has anti-inflammatory and analgesic effects. Xinyi still has anti-allergic effects. The lungs open to the nose, and the Yangming stomach veins up and down, the brain is the house of the Yuanshen, the nose is the gate of the life; the lack of gas in the people, the Qingyang does not rise, the head is tilted, the nine is the unfavorable. Xinyi's Xin Wen gas-filled into the lungs, can help the stomach in Qingyang to go up to the sky, so it can cure the head and nose of the disease.

Licorice: Adrenal cortical hormone-like effect, relieving cough and relieving asthma, regulating the body's immune function: licorice extract, glycyrrhizin and glycyrrhetinic acid have the effect of promoting sodium and water retention in healthy people and animals; low-dose glycyrrhizin (per Only 100ug) can atrophy the thymus gland and increase the weight of the adrenal gland, resulting in a cortisone-like effect of glucocorticoids. At high doses, the glucocorticoid-like effect is not obvious, and only mineralocorticoid-like effects are present. Cough and asthma, glycyrrhetinic acid has obvious central antitussive effect.

Uncaria: It has anti-histamine effect, and it has the effect of clearing heat and calming the liver and preventing wind and stopping phlegm.

White peony: There is a hurricane solution, through the hair, sputum, cold and pain, dampness and detoxification. Good treatment of the skin of the head and face, external treatment can treat pruritus. This product can reduce bronchial secretion, dilate coronary artery; can promote the absorption of damaged tissue, promote the absorption of chronic ulcer and skin inflammation or promote its purulent effect; have hemostasis, use it to treat sinusitis, chronic rhinitis is better Efficacy.

Ai Ye: There is warming and stopping bleeding, dispelling cold and relieving pain. Containing eucalyptus oil has the effect of relieving cough and relieving asthma. Frying can treat skin eczema, itching, hemorrhoids, etc.

Xu Changqing: Defying and defensive, dehumidifying and itching, has obvious anti-inflammatory and anti-allergic effects, can inhibit the biosynthesis of PGE2 in inflammatory tissues, and has obvious inhibitory effects on skin allergic reactions and contact dermatitis. It is alive, phlegm and cold, clear blood, antipyretic and anti-allergic effects, has a certain inhibitory effect on delayed type hypersensitivity, has obvious anti-histamine effect on trachea, and has strong antithrombotic effect. .

Windproof: Hurricane solution, stop phlegm and relieve itching, anti-inflammatory and immunomodulatory effects, in vitro antibacterial test results against wind against Staphylococcus aureus, beta-hemolytic streptococcus, pneumococcus and two molds Mildew, Aspergillus variabilis, etc. have antibacterial action, but have no bacteriostatic action against influenza bacillus, typhoid bacillus, Fuchs and Shigella dysenteriae. It has obvious inhibitory effect on delayed hypersensitivity reaction in mice induced by dinitrochlorobenzene, and can reduce allergic contraction of isolated tracheal and ileal smooth muscle in sensitized guinea pigs.

Propolis: antibacterial, anti-viral, enhance immunity, anti-oxidation. Propolis can be sterilized, sterilized, disinfected, bacteriostatic, mildewproof, and antiseptic. It solves the defect that antibiotics only act on a single microorganism, and has no side effects. Propolis is a natural antibiotic Viral substances have a strong inhibitory and killing effect on a variety of viruses. Known as antioxidants and free radical scavengers, propolis can effectively remove excess reactive oxygen and free radicals such as obesity, overwork, environmental pollution, smoking and other unhealthy living habits and external factors. The body's immune system is easily attacked by viruses. Propolis can enhance the body's immunity and enhance the body's ability to resist virus damage.

In the whole Chinese medicine formula, Asarum, Wild Chrysanthemum, Sophora flavescens, Magnolia, and Radix Paeoniae Alba are used as the drug group, with Xu Changqing, Ai Ye, and Fengshi as the drug group, accompanied by Astragalus, Pinellia, Honeysuckle, Bitter Almond, and Artemisia, Licorice and propolis are used as medicine groups. Achieve the qi stagnation, warm lungs and nose, clearing away heat and detoxification, dehumidification and sputum, hurricane solution and other effects.

Modern Chinese medicine believes that endowment is insufficient, and viscera disorders, especially the lung, spleen and kidney, are the pathogenesis of CARAS; the cold and the evil of the qi are the cause of the disease, so the CARAS caused by the cold of the lung and spleen It is necessary to desensitize Wenyang Yiqi to achieve the effect of treating CARAS. According to this theory, a safe, effective, and long-term application of a novel Chinese medicine preparation is provided in the present invention. Since the active ingredients in each medicinal material are different, the most suitable extraction method is not the same. According to the needs of different medicinal materials, different extraction methods are selected, and further appropriate combination is adopted. On the basis of a large number of experiments, the applicant preferably selects the weight ratio to ensure the best therapeutic effect.

The invention also provides a method for preparing the traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome, comprising the following steps:

1) mixing the original medicinal materials according to the number of parts, washing and pulverizing into a powdery solid;

2) 1 time by weight of the above powdery solid, added with 20 times by weight of water, decocted and heated for 30 minutes, then filtered through a 100-mesh screen to collect the filtrate; then concentrated under reduced pressure at a temperature of 55 ° C. The filtrate is concentrated under vacuum to obtain a leachate of the traditional Chinese medicine composition;

3) The leachate of the traditional Chinese medicine composition was spray-dried, and then pulverized into a solid powder of 100 mesh, and compressed into a pellet having a diameter of about 2 mm.

Beneficial effect

The traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome provided by the invention has volatile energy, and the traditional Chinese medicine composition can be absorbed by respiratory absorption and local skin tissue through specific, controlled and persistent cytopathitis Sexual reactions trigger an immune response that acts to desensitize, prevent and treat CARAS. The preparation method proposed by the invention is simple, safe and convenient.

BEST MODE FOR CARRYING OUT THE INVENTION

A traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome is composed of the following parts by weight: wild chrysanthemum 20, asarum 14, honeysuckle 20, astragalus 14, pinellia 14, matrine 12, bitter Almond 10, Xinyi 9, Licorice 11, Uncaria 17, White peony 14, Ai Ye 16, Xu Changqing 13, Wind 19, Propolis 18.

The raw materials are mixed according to the number of parts, washed, and then dried and pulverized into a powdery solid;

1 time by weight of the above powdery solid, added with 20 times by weight of water, decocted and heated for 30 minutes, then filtered through a 100-mesh sieve to collect the filtrate; then concentrated under reduced pressure at a temperature of 55 ° C under vacuum conditions Concentrating the filtrate to obtain a leachate of the traditional Chinese medicine composition;

The leachate of the traditional Chinese medicine composition was spray-dried, and then pulverized into a solid powder of 100 mesh, and compressed into a pellet having a diameter of 2 mm.

The animal test results of the present invention are as follows:

MATERIAL SOURCES: 69 male BALB/C mice (SPF grade) of 6-8 weeks weighing from 18g to 20g were provided by Beijing Vitalli Laboratory Animal Technology Co., Ltd. Grade V ovalbumin (OVA), Sigma. IL-12 antibody, Biosource. Animal experiments: Mice were randomly divided into a natural control group (group A, 21), an allergic rhinitis group (group B, 21) and a traditional Chinese medicine composition treatment group (group C, 28). Modeling method: using conventional OVA sensitization method, weigh the appropriate amount of OVA, heat inactivated B. pertussis and aluminum hydroxide in physiological saline to obtain sensitizing solution, mix before use; group B and C mice were intraperitoneally injected with sensitization Liquid, once every other day, a total of 7 times; group A was replaced by saline; on the 2nd day after sensitization, group B and group C were locally challenged with 5% OVA saline for 3 weeks, group A was replaced by normal saline. . Treatment: Group C was raised in an environment where the Chinese medicine composition was placed after challenge for 4 weeks; Groups A and B were kept in a conventional environment instead. The mice were sacrificed 4 weeks later, and the nasal mucosa was carefully removed under the microscope. The samples were quickly stored in formalin solution, and paraffin sections were prepared according to conventional tissue preparation techniques, and HE staining and IL-12 immunohistochemical staining were performed respectively. RESULTS: 1 Eosinophil count: The intact nasal mucosa was selected and the average was counted after 5 fields under high magnification (40×10). The score was: no or rare eosinophils (0~2) (0); eosinophils 3 to 10, 1 point; eosinophils 11 to 50, 2 points; eosinophils greater than 50, 3 points; 2 IL-12 staining: cytoplasm There were brown stained positive cells, and 5 fields were counted under high magnification (40×10) selection of nasal mucosa. The scoring standard: less than 5% of cells were positive and negative; the proportion of positive cells was 6%-20%, weak positive The proportion of positive cells is 21% to 40%, moderately positive; the proportion of positive cells is greater than 40%, strong positive. Statistical analysis: t-test and analysis of variance were used to compare eosinophil count data between groups. IL-12 staining results were counted by x2 test and correlation statistics were performed.

Table 1 Comparison of nasal mucosal eosinophil count scores in three groups of animals

Group (n)	0 marks	1 point	2 minutes	3 points	Positive rate (%)
A (21)	twenty one	-	-	-	0
B(20)	2	-	10	8	90
C(28)	20	4	2	-	21.4

Table 2 Comparison of IL-12 expression scores in nasal mucosa of three groups of animals

Group (n)	negative	Weak yang	Zhongyang	Strong yang	Positive rate (%)
A21	9	6	6	-	57.1
B20	16	2	2	-	20
C28	11	11	3	3	60.7

It can be seen that the formula can inhibit the immune inflammatory reaction of the respiratory tract, thereby suppressing the allergic phenomenon, and the animal medicine pillow is found to be non-irritating to the animal skin and the mucosal stimulation test result.

After 3 years of clinical treatment, the present invention puts 250 grams of traditional Chinese medicine composition into a sleeping pillow, and applies to 63 young and middle-aged people suffering from CARAS. Through routine examination and special examination, one month is a course of treatment. After 6 courses of treatment, each pillow was replaced with a pillow containing the Chinese medicine composition, and the total effective rate was 87.7%.

Industrial applicability

The invention can be used industrially for production. However, the present invention is not limited to the above-described contents, and it is within the scope of the present invention to fall within the scope of the present invention without departing from the scope of the present invention.

Claims (3)

1. A traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome is composed of the following parts by weight: wild chrysanthemum 16-20, asarum 9-16, honeysuckle 16-22, astragalus 9-14, half Xia 12～16, Kushen 7～13, bitter almond 8～11, Xinyi 7～11, licorice 9～17, Uncaria 11～18, Baiji 11～20, Aiye 11～16, Xu Changqing 6～14, windproof 14 ~22 parts, propolis 10-20.
2. The traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome according to claim 1, wherein the parts by weight of the components are: wild chrysanthemum 20, asarum 14, honeysuckle 20, and astragalus. Pinellia 14 , Sophora flavescens 12 , Bitter almond 10 , Xin Yi 9 , Licorice 11 , Uncaria 17 , White peony 14 , Ai Ye 16 , Xu Changqing 13 , Windproof 19 , Propolis 18 .
3. A method for preparing a traditional Chinese medicine composition for preventing and treating allergic rhinitis asthma syndrome according to claim 1 or 2, comprising the steps of:

   1) mixing the original medicinal materials according to the number of parts, washing and pulverizing into a powdery solid;

   2) 1 time by weight of the above powdery solid, added with 20 times by weight of water, decocted and heated for 30 minutes, then filtered through a 100-mesh screen to collect the filtrate; then concentrated under reduced pressure at a temperature of 55 ° C. The filtrate is concentrated under vacuum to obtain a leachate of the traditional Chinese medicine composition;

   3) The leachate of the traditional Chinese medicine composition was spray-dried, and then pulverized into a solid powder of 100 mesh, and pressed into a pellet having a diameter of 2 mm.