WO2018058865A1

WO2018058865A1 - Dynamic fluid shear technology-based cell-wall breaking method for spirulina

Info

Publication number: WO2018058865A1
Application number: PCT/CN2017/071539
Authority: WO
Inventors: 王峰; 俞建峰; 郑志永; 高志刚; 高楠
Original assignee: 东台市赐百年生物工程有限公司
Priority date: 2016-09-28
Filing date: 2017-01-18
Publication date: 2018-04-05
Also published as: CN106434351A

Abstract

Provided is a dynamic fluid shear technology-based cell-wall breaking method for spirulina, comprising the following steps: (1) sucking spirulina in the surface layer of a water body by means of a plurality of fixed vortex well spirulina obtaining devices provided in spirulina, and conveying the spirulina to a peak regulating tank through a closed pipeline, wherein the vortex well spirulina obtaining devices do not suck spirulina in the surface layer of the water body when the concentration of spirulina slurry in the closed pipeline is lower than a set concentration; (2) conveying the spirulina into an ultrasonic cell disruptor, shearing spirulina cells, and carrying out ultrasonic disruption; and (3) conveying the disrupted spirulina to a low-temperature centrifuge by means of a feeding pump for centrifugation to separate the spirulina from water, filtering by means of multiple layers of scrims, and extruding to obtain purple red juice, discharging spirulina residues through a discharging opening, and recycling water through a pipeline.

Description

Spirulina breaking method based on fluid dynamic shearing technology

Technical field

The invention relates to the technical field of biological cell breaking, and particularly relates to a spirulina breaking method based on fluid dynamic shearing technology.

Background technique

Spirulina is a low-grade miniature cyanobacteria with nutrient-care function. It has a high protein content of 70% of dry weight and is rich in most of the amino acids needed by the human body to meet the needs of human survival. Spirulina contains 18 kinds of amino acids, among which the 8 essential amino acids are reasonable in structure, almost similar to human blood proteins, and the RDA values recommended by the Food and Agriculture Organization of the United Nations (FAO) and the World Health Organization (WHO). Spirulina is a natural treasure trove of vitamins and contains many beneficial minerals such as calcium, iron, zinc, selenium, potassium, magnesium and iodine. Spirulina is rich in human body and cannot be synthesized. Linoleic acid and linolenic acid, as well as phycocyanin, chlorophyll, small molecule polysaccharide, superoxide dismutase, pseudo-growth factor and other biologically active substances. However, the bioactive substances of spirulina are contained in the cell wall with a special structure, which affects the nutritional value of spirulina to some extent. Therefore, the spirulina's broken wall is of great significance to improve its bioavailability.

Spirulina is a single cell in a spiral shape, which has a filament length of 200 to 500 μm and a width of 5 to 10 μm. Due to the small volume, it is difficult to break the wall by ordinary pulverization methods. Pharmaceutical grade spirulina is about 200,000 yuan per ton, and feed grade is 6 to 80,000 yuan per ton. Therefore, the research on the spirulina wall breaking has certain economic value.

Summary of the invention

The object of the present invention is to provide a spirulina wall breaking method based on a fluid dynamic shearing technique with high phycocyanin yield, in view of the above problems.

In order to achieve the above object, the present invention adopts the following technical solution: a method for breaking a spirulina based on a fluid dynamic shearing technique of the present invention, comprising the following steps:

(1) Using a plurality of vortex wells set in the fixed spirulina to absorb the spirulina on the surface of the water body, and transport it to the peaking tank through the closed pipeline, when the concentration of spirulina in the sealed pipeline is lower than the set concentration When the vortex well agitator does not absorb the spirulina on the surface of the water body;

(2) transported to an ultrasonic cell pulverizer, shearing the spirulina cells, and pulverizing by ultrasonic waves;

(3) After pulverization, the feed pump is sent to the low-temperature centrifuge for centrifugation, and the spirulina is separated from the water. The multi-layer gauze is filtered and squeezed to obtain a purple-red juice; the spirulina slag is discharged through the discharge port, and the water passes through. Pipeline reuse.

Further, in the step (2), the treatment time of the ultrasonic cell pulverizer is 32-40 min.

Further, in the step (2), the spirulina cell has a crushing particle size of 300 mesh or more.

Further, in the step (3), the processing time of the cryogenic centrifuge is 20-30 min, and the treated temperature is 4-6 °C.

Further, in the step (3), the rotational speed of the cryogenic centrifuge is 7000-8000 r/min.

The invention has the advantages that the method based on the fluid dynamic shearing technique is simple and convenient, and the feasibility is strong, and the spirulina after the crushing can enter the subsequent phycocyanin extraction process flow, and the spirulina phycocyanin yield extracted by the fluid dynamic shearing And high purity. Ultrasonic breaking method is a strong cell breaking method. In spirulina fluid, dynamic shearing, the breaking mechanism is the shearing force and shock wave generated by cavitation, making fine The cells are fully broken and the protein is fully released. The experiment proved that after 2 cycles, the spirulina cell crushing particle size reached more than 300 mesh.

DRAWINGS

Figure 1 is a map showing the relationship between the purity of spirulina and the breaking time of the present invention;

detailed description

The following examples are for illustrative purposes only and are not intended to limit the scope of the invention.

Example 1

The method for spirulina breaking according to the fluid dynamic shearing technique of the invention comprises the following steps:

(2) It is transported to an ultrasonic cell pulverizer, and the spirulina cells are subjected to shear treatment, and ultrasonic waves are pulverized; the processing time of the ultrasonic cell pulverizer is 32 min. The spirulina cell has a crushing particle size of 300 mesh or more.

(3) After pulverization, the feed pump is sent to the low-temperature centrifuge for centrifugation, and the spirulina is separated from the water. The multi-layer gauze is filtered and squeezed to obtain a purple-red juice; the spirulina slag is discharged through the discharge port, and the water passes through. Pipeline reuse. The processing time of the cryogenic centrifuge was 25 min and the treated temperature was 5 °C. The speed of the cryogenic centrifuge was 7500 r/min.

Example 2

The difference between Embodiment 2 and Embodiment 1 is that:

In the step (2), the mixture is transferred to an ultrasonic cell pulverizer, and the spirulina cells are subjected to shear treatment, and ultrasonic waves are pulverized; and the treatment time of the ultrasonic cell pulverizer is 40 minutes.

In the step (3), after being pulverized and transported to a low-temperature centrifuge by a feed pump, the spirulina is separated from the water, and the multi-layer gauze is filtered and squeezed to obtain a purple-red juice; the spirulina slag is discharged through the discharge port. Out, the water is reused through the pipeline. The processing time of the cryogenic centrifuge was 20 min and the treated temperature was 4 °C. The low temperature centrifuge has a rotational speed of 8000 r/min.

Example 3

The difference between Embodiment 3 and Embodiment 1 is:

In the step (2), the mixture is transferred to an ultrasonic cell pulverizer, and the spirulina cells are subjected to shear treatment, and ultrasonic waves are pulverized; and the treatment time of the ultrasonic cell pulverizer is 38 minutes.

In the step (3), after being pulverized and transported to a low-temperature centrifuge by a feed pump, the spirulina is separated from the water, and the multi-layer gauze is filtered and squeezed to obtain a purple-red juice; the spirulina slag is discharged through the discharge port. Out, the water is reused through the pipeline. The processing time of the cryogenic centrifuge was 30 min and the treated temperature was 6 °C. The low temperature centrifuge has a rotational speed of 7000 r/min.

Test 1

Example 1 was added to a 0.5% (w/v) CaCl ₂ solution as an extraction solvent, and the experiment was carried out at different sonication times. The optimal configuration time is shown in Figure 1. As shown in Fig. 1, the purity of spirulina increases with the increase of the crushing time, and slowly decreases after reaching the highest value. At 35 min, the purity reached 0.6 and the yield slowly increased with increasing break time. After analysis, the longer the crushing time, the more complete the spirulina breaks, and the higher the purity and yield of the obtained phycocyanin.

The specific embodiments described herein are merely illustrative of the spirit of the invention. A person skilled in the art can make various modifications or additions to the specific embodiments described. The invention is replaced in a similar manner, without departing from the spirit of the invention or beyond the scope of the appended claims.

Claims

A spirulina breaking method based on fluid dynamic shearing technology, comprising the following steps:

(1) Using a plurality of vortex wells set in the fixed spirulina to absorb the spirulina on the surface of the water body, and transport it to the peaking tank through the closed pipeline, when the concentration of spirulina in the sealed pipeline is lower than the set concentration When the vortex well agitator does not absorb the spirulina on the surface of the water body;

(2) transported to an ultrasonic cell pulverizer, shearing the spirulina cells, and pulverizing by ultrasonic waves;

(3) After pulverization, the feed pump is sent to the low-temperature centrifuge for centrifugation, and the spirulina is separated from the water. The multi-layer gauze is filtered and squeezed to obtain a purple-red juice; the spirulina slag is discharged through the discharge port, and the water passes through. Pipeline reuse.
The spirulina breaking method based on the fluid dynamic shearing technique according to claim 1, wherein in the step (2), the processing time of the ultrasonic cell pulverizer is 32-40 min.
The spirulina wall breaking method based on the fluid dynamic shearing technique according to claim 2, wherein in the step (2), the spirulina cell has a crushing particle size of 300 mesh or more.
The spirulina breaking method based on fluid dynamic shearing technology according to claim 1, wherein in the step (3), the processing time of the cryogenic centrifuge is 20-30 min, and the processing temperature is 4- 6 ° C.
The spirulina breaking method based on the fluid dynamic shearing technique according to claim 4, wherein in the step (3), the rotational speed of the cryogenic centrifuge is 7000-8000 r/min.