WO2018048957A3 - Normalisation de concentration de banque ngs - Google Patents

Normalisation de concentration de banque ngs Download PDF

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Publication number
WO2018048957A3
WO2018048957A3 PCT/US2017/050354 US2017050354W WO2018048957A3 WO 2018048957 A3 WO2018048957 A3 WO 2018048957A3 US 2017050354 W US2017050354 W US 2017050354W WO 2018048957 A3 WO2018048957 A3 WO 2018048957A3
Authority
WO
WIPO (PCT)
Prior art keywords
normalization
ngs library
library concentration
ngs
methods
Prior art date
Application number
PCT/US2017/050354
Other languages
English (en)
Other versions
WO2018048957A2 (fr
Inventor
Vladimir Makarov
Sergey CHUPRETA
Original Assignee
Swift Biosciences, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Swift Biosciences, Inc. filed Critical Swift Biosciences, Inc.
Priority to AU2017324946A priority Critical patent/AU2017324946C1/en
Priority to CA3035698A priority patent/CA3035698A1/fr
Priority to CN201780068285.4A priority patent/CN110012671B/zh
Priority to JP2019512800A priority patent/JP7001678B2/ja
Priority to EP17849489.4A priority patent/EP3510155A4/fr
Publication of WO2018048957A2 publication Critical patent/WO2018048957A2/fr
Publication of WO2018048957A3 publication Critical patent/WO2018048957A3/fr
Priority to US16/294,561 priority patent/US10961562B2/en
Priority to US17/214,251 priority patent/US11697836B2/en
Priority to JP2021210770A priority patent/JP7439041B2/ja
Priority to US18/322,200 priority patent/US20240068011A1/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Un obstacle dans le flux de travail de séquençage de nouvelle génération (NGS) est la quantification de banques pour une mise en commun et un chargement précis de la cellule ou de la puce de circulation d'instrument de séquençage. L'invention concerne des procédés qui améliorent les performances et réduisent le temps par rapport aux procédés existants.
PCT/US2017/050354 2016-09-06 2017-09-06 Normalisation de concentration de banque ngs WO2018048957A2 (fr)

Priority Applications (9)

Application Number Priority Date Filing Date Title
AU2017324946A AU2017324946C1 (en) 2016-09-06 2017-09-06 Normalization of NGS library concentration
CA3035698A CA3035698A1 (fr) 2016-09-06 2017-09-06 Normalisation de concentration de banque ngs
CN201780068285.4A CN110012671B (zh) 2016-09-06 2017-09-06 Ngs文库浓度的标准化
JP2019512800A JP7001678B2 (ja) 2016-09-06 2017-09-06 Ｎｇｓライブラリー濃度の正規化
EP17849489.4A EP3510155A4 (fr) 2016-09-06 2017-09-06 Normalisation de concentration de banque ngs
US16/294,561 US10961562B2 (en) 2016-09-06 2019-03-06 Normalization of NGS library concentration
US17/214,251 US11697836B2 (en) 2016-09-06 2021-03-26 Normalization of NGS library concentration
JP2021210770A JP7439041B2 (ja) 2016-09-06 2021-12-24 Ｎｇｓライブラリー濃度の正規化
US18/322,200 US20240068011A1 (en) 2016-09-06 2023-05-23 Normalization of ngs library concentration

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662384118P 2016-09-06 2016-09-06
US62/384,118 2016-09-06

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US16/294,561 Continuation US10961562B2 (en) 2016-09-06 2019-03-06 Normalization of NGS library concentration

Publications (2)

Publication Number Publication Date
WO2018048957A2 WO2018048957A2 (fr) 2018-03-15
WO2018048957A3 true WO2018048957A3 (fr) 2018-05-31

Family

ID=61561643

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2017/050354 WO2018048957A2 (fr) 2016-09-06 2017-09-06 Normalisation de concentration de banque ngs

Country Status (7)

Country Link
US (3) US10961562B2 (fr)
EP (1) EP3510155A4 (fr)
JP (2) JP7001678B2 (fr)
CN (1) CN110012671B (fr)
AU (1) AU2017324946C1 (fr)
CA (1) CA3035698A1 (fr)
WO (1) WO2018048957A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110012671B (zh) * 2016-09-06 2024-05-28 合成Dna技术公司 Ngs文库浓度的标准化
KR102159008B1 (ko) * 2019-10-18 2020-09-23 고려대학교 산학협력단 자가 증폭이 가능한 헤어핀 구조의 ｎｇｓ 라이브러리 제작용 어댑터 및 이를 이용한 ｎｇｓ 라이브러리 제조방법
US11365411B2 (en) * 2020-01-21 2022-06-21 Microsoft Technology Licensing, Llc Efficient random access to DNA-encoded data
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用
CN113588392B (zh) * 2021-07-28 2022-03-04 北京金匙基因科技有限公司 一种改善测序混样均匀性的定量混样方法
WO2023107899A2 (fr) * 2021-12-07 2023-06-15 Caribou Biosciences, Inc. Procédé de capture de produits du clivage de l'endonucléase crispr

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US20140378317A1 (en) * 2007-09-14 2014-12-25 Affymetrix, Inc. Amplification and Analysis of Selected Targets on Solid Supports
WO2015134552A1 (fr) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Ligature d'adaptateur améliorée
US20160046987A1 (en) * 2014-08-14 2016-02-18 Abbott Molecular Inc. Library generation for next-generation sequencing

Family Cites Families (12)

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Publication number Priority date Publication date Assignee Title
US6297010B1 (en) * 1998-01-30 2001-10-02 Genzyme Corporation Method for detecting and identifying mutations
WO2001061036A2 (fr) * 2000-02-17 2001-08-23 Complete Genomics As Procede de cartographie de sites de coupure d'endonuclease de restriction
EP1604040B1 (fr) * 2003-03-07 2010-10-13 Rubicon Genomics, Inc. Amplification et analyse d'un genome entier et de bibliotheques de transcriptomes entiers generees par un procede de polymerisation d'adn
WO2009043112A1 (fr) * 2007-10-04 2009-04-09 Commonwealth Scientific And Industrial Research Organisation Amplification d'acide nucléique
US9540637B2 (en) * 2008-01-09 2017-01-10 Life Technologies Corporation Nucleic acid adaptors and uses thereof
US8586310B2 (en) * 2008-09-05 2013-11-19 Washington University Method for multiplexed nucleic acid patch polymerase chain reaction
AU2013232131B2 (en) * 2012-03-13 2018-09-20 Integrated Dna Technologies, Inc. Methods and compositions for size-controlled homopolymer tailing of substrate polynucleotides by a nucleic acid polymerase
SG11201408478QA (en) * 2012-06-18 2015-02-27 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
JP6321318B2 (ja) * 2012-09-14 2018-05-09 日本碍子株式会社 標的核酸の検出方法
US11021702B2 (en) * 2012-09-25 2021-06-01 Agriculture Victoria Services Pty Ltd Method of producing a normalised nucleic acid library using solid state capture material
CN104830961B (zh) * 2014-02-12 2018-05-01 大江基因医学股份有限公司 用于活体外测定致肥胖风险的方法
CN110012671B (zh) * 2016-09-06 2024-05-28 合成Dna技术公司 Ngs文库浓度的标准化

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4988617A (en) * 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US20140378317A1 (en) * 2007-09-14 2014-12-25 Affymetrix, Inc. Amplification and Analysis of Selected Targets on Solid Supports
WO2015134552A1 (fr) * 2014-03-03 2015-09-11 Swift Biosciences, Inc. Ligature d'adaptateur améliorée
US20160046987A1 (en) * 2014-08-14 2016-02-18 Abbott Molecular Inc. Library generation for next-generation sequencing

Also Published As

Publication number Publication date
CN110012671B (zh) 2024-05-28
JP2019526267A (ja) 2019-09-19
CN110012671A (zh) 2019-07-12
AU2017324946C1 (en) 2022-06-09
US11697836B2 (en) 2023-07-11
EP3510155A2 (fr) 2019-07-17
JP7001678B2 (ja) 2022-02-04
US20240068011A1 (en) 2024-02-29
WO2018048957A2 (fr) 2018-03-15
US20210277447A1 (en) 2021-09-09
AU2017324946B2 (en) 2022-02-24
US20190211374A1 (en) 2019-07-11
AU2017324946A1 (en) 2019-03-21
US10961562B2 (en) 2021-03-30
JP7439041B2 (ja) 2024-02-27
JP2022044605A (ja) 2022-03-17
EP3510155A4 (fr) 2020-03-18
CA3035698A1 (fr) 2018-03-15

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