WO2018046312A3 - Perfectionnements apportés ou liés au silençage génique - Google Patents

Perfectionnements apportés ou liés au silençage génique Download PDF

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Publication number
WO2018046312A3
WO2018046312A3 PCT/EP2017/071388 EP2017071388W WO2018046312A3 WO 2018046312 A3 WO2018046312 A3 WO 2018046312A3 EP 2017071388 W EP2017071388 W EP 2017071388W WO 2018046312 A3 WO2018046312 A3 WO 2018046312A3
Authority
WO
WIPO (PCT)
Prior art keywords
gene silencing
relating
dsrna
gene
carbohydrate
Prior art date
Application number
PCT/EP2017/071388
Other languages
English (en)
Other versions
WO2018046312A2 (fr
Inventor
Myriam Beghyn
Yann Naudet
Lies Degrave
Lien DE SCHRIJVER
Kevin V DONOHUE
Original Assignee
Syngenta Participations Ag
Devgen Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syngenta Participations Ag, Devgen Nv filed Critical Syngenta Participations Ag
Publication of WO2018046312A2 publication Critical patent/WO2018046312A2/fr
Publication of WO2018046312A3 publication Critical patent/WO2018046312A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
    • A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
    • A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

La présente invention concerne une composition pesticide comprenant un acide ribonucléique double brin (ARNdb) et un glucide, ledit ARNdb permettant le silençage génique d'ARNi et comprenant un brin qui est complémentaire d'au moins une partie d'une séquence nucléotidique d'un gène provenant d'un organisme nuisible végétal.
PCT/EP2017/071388 2016-09-06 2017-08-24 Perfectionnements apportés ou liés au silençage génique WO2018046312A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662383666P 2016-09-06 2016-09-06
US62383666 2016-09-06

Publications (2)

Publication Number Publication Date
WO2018046312A2 WO2018046312A2 (fr) 2018-03-15
WO2018046312A3 true WO2018046312A3 (fr) 2018-04-19

Family

ID=59738332

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/EP2017/071388 WO2018046312A2 (fr) 2016-09-06 2017-08-24 Perfectionnements apportés ou liés au silençage génique

Country Status (2)

Country Link
AR (1) AR109465A1 (fr)
WO (1) WO2018046312A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115161306B (zh) * 2022-06-29 2023-09-15 江苏省农业科学院 绿盲蝽rna降解酶、其编码基因、载体、菌株及其应用

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003004644A1 (fr) * 2001-07-06 2003-01-16 Commonwealth Scientific And Industrial Research Organisation Administration d'arn bicatenaires a des arthropodes
WO2005049841A1 (fr) * 2003-11-17 2005-06-02 Commonwealth Scientific And Industrial Research Organisation Resistance aux insectes par inhibition de l'expression genique
EP2270182A2 (fr) * 2005-09-16 2011-01-05 deVGen N.V. l'ARN à double brin pour la lutte contre les insectes
WO2012055982A2 (fr) * 2010-10-27 2012-05-03 Devgen Nv Régulation à la baisse de l'expression génique chez des insectes nuisibles
US20120148524A1 (en) * 2010-12-14 2012-06-14 Vander Meer Robert K Formicidae (Ant) Control Using Double-Stranded RNA Constructs
WO2012143542A1 (fr) * 2011-04-20 2012-10-26 Devgen Nv Diminution de l'expression génique chez des insectes nuisibles
WO2015153339A2 (fr) * 2014-04-01 2015-10-08 Monsanto Technology Llc Compositions et procédés pour lutter contre les insectes nuisibles
US20160053259A1 (en) * 2014-08-22 2016-02-25 The United States Of America As Represented By The Secretary Of Agriculture Double stranded rna constructs for aphid control

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003004644A1 (fr) * 2001-07-06 2003-01-16 Commonwealth Scientific And Industrial Research Organisation Administration d'arn bicatenaires a des arthropodes
WO2005049841A1 (fr) * 2003-11-17 2005-06-02 Commonwealth Scientific And Industrial Research Organisation Resistance aux insectes par inhibition de l'expression genique
EP2270182A2 (fr) * 2005-09-16 2011-01-05 deVGen N.V. l'ARN à double brin pour la lutte contre les insectes
WO2012055982A2 (fr) * 2010-10-27 2012-05-03 Devgen Nv Régulation à la baisse de l'expression génique chez des insectes nuisibles
US20120148524A1 (en) * 2010-12-14 2012-06-14 Vander Meer Robert K Formicidae (Ant) Control Using Double-Stranded RNA Constructs
WO2012143542A1 (fr) * 2011-04-20 2012-10-26 Devgen Nv Diminution de l'expression génique chez des insectes nuisibles
WO2015153339A2 (fr) * 2014-04-01 2015-10-08 Monsanto Technology Llc Compositions et procédés pour lutter contre les insectes nuisibles
US20160053259A1 (en) * 2014-08-22 2016-02-25 The United States Of America As Represented By The Secretary Of Agriculture Double stranded rna constructs for aphid control

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
HADA WURIYANGHAN ET AL: "Oral Delivery of Double-Stranded RNAs and siRNAs Induces RNAi Effects in the Potato/Tomato Psyllid, Bactericerca cockerelli", PLOS ONE, vol. 6, no. 11, 16 November 2011 (2011-11-16), pages e27736, XP055068898, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0027736 *
SHIM JAE-KYOUNG ET AL: "Oral ingestion of heat shock protein 70 dsRNA is lethal under normal and thermal stress conditions in the sweetpotato whitefly,Bemisia tabaci", JOURNAL OF ASIA PACIFIC ENTOMOLOGY, KOREAN SOCIETY OF APPLIED ENTOMOLOGY, SUWON, KR, vol. 18, no. 4, 17 October 2015 (2015-10-17), pages 797 - 800, XP029318346, ISSN: 1226-8615, DOI: 10.1016/J.ASPEN.2015.10.004 *
TURNER C T ET AL: "RNA interference in the light brown apple moth, Epiphyas postvittana (Walker) induced by double-stranded RNA feeding", INSECT MOLECULAR BIO, BLACKWELL SCIENTIFIC, OXFORD, GB, vol. 15, no. 3, 31 May 2006 (2006-05-31), pages 383 - 391, XP008082514, ISSN: 0962-1075, DOI: 10.1111/J.1365-2583.2006.00656.X *
WHYARD S ET AL: "Ingested double-stranded RNAs can act as species-specific insecticides", INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, ELSEVIER SCIENCE LTD, GB, vol. 39, no. 11, 6 October 2009 (2009-10-06), pages 824 - 832, XP026777046, ISSN: 0965-1748, [retrieved on 20091006], DOI: 10.1016/J.IBMB.2009.09.007 *

Also Published As

Publication number Publication date
AR109465A1 (es) 2018-12-12
WO2018046312A2 (fr) 2018-03-15

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