WO2018029890A1

WO2018029890A1 - Antibiotic

Info

Publication number: WO2018029890A1
Application number: PCT/JP2017/012319
Authority: WO
Inventors: 靖史田中
Original assignee: 株式会社ブレインヘルス
Priority date: 2016-08-09
Filing date: 2017-03-27
Publication date: 2018-02-15
Also published as: JP2018024592A

Abstract

Provided is an antibiotic capable of showing an immediate effect. The present invention relates to an antibiotic that comprises gnetin C, resveratrol, vitamin E and a fluidizing agent. The content by weight of gnetin C is 0.17-4.11 wt% inclusive. The content by weight of resveratrol is 0.06-1.33 wt% inclusive. The content by weight of vitamin E is 0.28-2.22 wt% inclusive. It is preferred that the content by weight of gnetin C is 0.17-1.37 wt% inclusive. In this case, it is preferred that the content by weight of resveratrol is 0.06-0.44 wt% inclusive. It is still preferred that the sum of the contents by weight of gnetin C and resveratrol is 0.23-1.81 wt% inclusive.

Description

Antibiotics

The present invention relates to antibiotics.

Patent Document 1 discloses an agent for preventing influenza virus infection. The preventive agent for influenza virus infection disclosed in Patent Document 1 uses tea leaves as an active ingredient. The influenza virus infection preventive agent disclosed in Patent Document 1 can effectively prevent virus infection without depending on antigenicity, and has no harmful side effects on the human body.

Patent Document 2 discloses a pharmaceutical composition. The pharmaceutical composition disclosed in Patent Document 2 contains a modified catechin. The pharmaceutical composition disclosed in Patent Document 2 can increase antibacterial activity.

Japanese Patent Laid-Open No. 3-101623 Special table 2007-521239 gazette

However, the preventive agent for influenza virus infection disclosed in Patent Document 1 and the pharmaceutical composition disclosed in Patent Document 2 have a problem in that the onset of the effect is slow.

The object of the present invention is to provide antibiotics that are effective quickly.

As a result of earnest research to solve the above-mentioned problems, the present inventor has found that antibiotics containing melinjo extract and vitamin E can quickly suppress the activity of bacteria and viruses, and completed the present invention.

That is, the present invention relates to antibiotics. This antibiotic includes gnetin C, resveratrol, vitamin E, and a fluidizing substance. The weight% of gnetin C is 0.17 wt% or more and 4.11 wt% or less. The weight% of resveratrol is 0.06 wt% or more and 1.33 wt% or less. The weight% of vitamin E is 0.28 wt% or more and 2.22 wt% or less.

Moreover, it is preferable that the weight% of the above-mentioned gnetin C is 0.17 wt% or more and 1.37 wt% or less. In this case, it is preferable that the weight% of resveratrol is 0.06 weight% or more and 0.44 weight% or less.

Or it is preferable that the sum of the weight% of the above-mentioned gnetin C and the weight% of resveratrol is 0.23% by weight or more and 1.81% by weight or less.

Moreover, it is preferable that the weight% of the above-mentioned gnetin C is 0.17 wt% or more and 0.69 wt% or less. In this case, it is preferable that the weight% of resveratrol is 0.06 weight% or more and 0.22 weight% or less.

The antibiotic of the present invention is effective quickly.

MDCK. 2 When the cells were placed in the collagen gel coat preparation indentation, the MDCK. It is a microscope picture of 2 cells. MDCK. 2 When the cells were put into the collagen gel coat preparation indentation, the MDCK. It is a microscope picture of 2 cells. MDCK. The MDCK.2 at 100 minutes after the 2 cells were placed in the preparation of the collagen gel coat preparation. It is a microscope picture of 2 cells. MDCK. 2 When the cells were placed in the collagen gel coat preparation indentation 120 minutes later, the MDCK. It is a microscope picture of 2 cells. MDCK. 2 When the cells were placed in the collagen gel coat preparation indentation 150 minutes later, their MDCK. It is a microscope picture of 2 cells. MDCK. It is the figure which showed the microscope picture of 2 cells along the time series. MDCK. 2 When the cells were placed in the collagen gel coat preparation indentation, the MDCK. It is a microscope picture of 2 cells. MDCK. 2 When the cells were put into the collagen gel coat preparation indentation, the MDCK. It is a microscope picture of 2 cells. MDCK. 2 When the cells were placed in the collagen gel coat preparation indentation 120 minutes later, the MDCK. It is a microscope picture of 2 cells. MDCK. 2 MDCK. Of the cells at the time when 180 minutes have passed since the cells were put into the preparation of the collagen gel coat preparation. It is a microscope picture of 2 cells. MDCK. It is the figure which showed the microscope picture of 2 cells along the time series.

[Description of ingredients]
The present invention will be described in detail below. Antibiotics of the present invention include gnetin C, resveratrol, vitamin E, and a fluidizing substance.

(Description of Gunnetin C)
Gnetin C is a dimer of resveratrol. The structural formula of gnetin C is well known and will not be repeated here. Gnetin C is contained, for example, in the fruit of Merinjo (scientific name: Gnetum gnemon). In the present invention, the weight% of gnetin C is 0.17 wt% or more and 4.11 wt% or less. Preferably, the weight% of gnetin C is 0.17 wt% or more and 1.37 wt% or less. More preferably, the weight% of gnetin C is 0.17 wt% or more and 0.69 wt% or less.

(Description of resveratrol)
Resveratrol is a kind of stilbene derivative. The structural formula of resveratrol is well known and will not be repeated here. Resveratrol is found in red wine, red grape skin, peanut skin, itadori, melinjo and others. In the present invention, the weight percent of resveratrol is 0.06 wt% or more and 1.33 wt% or less. Preferably, the weight percent of resveratrol is 0.06 wt% or more and 0.44 wt% or less. More preferably, the weight percent of resveratrol is 0.06 wt% or more and 0.22 wt% or less.

It should be noted that when the weight% of gnetin C is 0.17 wt% or more and 1.37 wt% or less and the weight% of resveratrol is 0.06 wt% or more and 0.44 wt% or less, It is preferable that the sum of the weight percent of the above and the weight percent of resveratrol is 0.23% by weight or more and 1.81% by weight or less.

(Explanation of vitamin E)
Since the structure of vitamin E is well known, its description is not repeated here. In the present invention, the vitamin E weight percent is 0.28 wt% or more and 2.22 wt% or less. Preferably, the vitamin E weight percent is 0.28 wt% or more and 1.11 wt% or less.

(Description of fluidized material)
The fluidizing substance is a substance that facilitates the flow of the antibiotic of the present invention. The specific components of the fluidizing substance are not particularly limited as long as the synergistic effects of gnetin C, resveratrol, and vitamin E are not completely lost. However, the fluidizing substance is preferably a substance that does not impair the synergistic effect of gnetin C, resveratrol, and vitamin E. The fluidizing substance is more preferably a substance that enhances the synergistic effect of gnetin C, resveratrol, and vitamin E. Examples of fluidizing materials include dimethyl sulfoxide and purified water.

(Description of other ingredients)
Antibiotics of the present invention may contain components other than gnetin C, resveratrol, vitamin E, and fluidizing substances. An example of such a component is Gnetin C glycoside. Examples of glycosides of gnetin C include gunemonoside A, gunemonoside C, and gunemonoside D. The specific content of such components is not particularly limited as long as the synergistic effect of gnetin C, resveratrol, and vitamin E is not completely lost. Of course, the antibiotic of the present invention may consist only of gnetin C, resveratrol, vitamin E, and a fluidizing substance.

[Description of manufacturing method]
The method for producing the antibiotic of the present invention is not particularly limited. One example includes an extract extraction process, a fruit crushing process, a vitamin E addition process, and a fluidization process. The extract extraction step is a step of extracting the meringo extract from the fruit of meringo. Melinjo extract is an extract of the fruit of Merinjo. Melinjo extract contains gnetin C and resveratrol. The fruit crushing step is a step of crushing the fruit of Merinjo. Merinjo fruit is pulverized before the meringo extract is extracted from merinjo fruit in the extract extraction process. The vitamin E addition step is a step of adding vitamin E to the meringo extract after the extract extraction step. The fluidizing step is a step of imparting fluidity to the meringo extract.

[Description of usage and usage]
The antibiotic according to the present invention can be used for various applications described below. Its uses are the control of bacteria and viruses, their disinfection, and their growth inhibition. The range of bacteria and viruses targeted by the antibiotic according to the present invention is not particularly limited. Antibiotics according to the present invention can combat various bacteria and various viruses. The antibiotic according to the present invention can be used in various ways. Examples include internal medicines, paints, poultices, and sprays.

Hereinafter, the present invention will be specifically described with reference to examples. However, the present invention is not limited to these.

[Example 1]
(1) Test Procedure (A) Preparation of Melinjo Extract Tube The worker prepared a Melinjo extract tube. The “merinjo extract tube” is a tube in which a purified merinjo extract solution described later is contained in a predetermined tube described later. The operator prepared the meringo extract tube according to this example according to the following procedure. First, the worker roasted the fruit of Melinjo (scientific name: Gnetum gnemon) at 110 degrees Celsius for 10 minutes. The mass of merinjo fruit for one meringo extract tube was 50 grams. By roasting, the water content of the melinjo was 8% by weight. The meringo was made by pulverizing the whole skin, fruit and seeds. When the roasting was finished, the operator soaked the roasted melinjo in 55% by weight ethyl alcohol in a sealed bottle for 24 hours. The volume of ethyl alcohol for one meringo extract tube was 500 ml. While the meringo was soaked, the temperature of the ethyl alcohol was maintained at 90 degrees Celsius. While the meringo was soaked, the ethyl alcohol continued to be stirred by the stirrer. After the meringo was soaked in ethyl alcohol for 24 hours, the worker extracted an unpurified meringo extract from the ethyl alcohol supernatant. A centrifugal evaporator (EC-57C manufactured by Sakuma Seisakusho Co., Ltd.) was used for extraction of the unpurified melinjo extract. The extraction time was 12 hours. The unpurified meringo extract extracted by the centrifugal evaporator was in the form of a dry powder. When the unpurified melinjo extract was extracted, the worker added an aqueous dimethyl sulfoxide solution (the concentration of dimethyl sulfoxide was 63% by weight) to the unpurified melinjo extract and stirred. The amount of the aqueous dimethyl sulfoxide solution added was 3 ml with respect to 5 ml of the supernatant of ethyl alcohol from which the unpurified meringo extract was extracted. The unpurified meringo extract to which the aqueous dimethyl sulfoxide solution has been added and stirred is the unpurified meringo extract solution in this example. When the stirring was completed, the operator removed impurities from the unpurified melinjo extract solution by using a centrifuge (Eppendorf Model 5415R). The rotational speed of this centrifuge was 15400 rpm. This centrifuge was used for 10 minutes. By removing the impurities, the unpurified meringo extract solution became a purified meringo extract solution. The operator accommodated 2 grams (30 milliliters) of this purified meringo extract solution in a predetermined tube. This is a meringo extract tube. The purified meringo extract solution contained in one meringo extract tube is composed of 0.46% by weight of gnetin C, 0.15% by weight of resveratrol, 62.62% by weight of dimethyl sulfoxide, and water content. 36.77% by weight.

(B) Preparation of vitamin tube The operator put 50 grams of vitamin E powder (manufactured by Yamaguchi Kaken Co., Ltd.) into a bottle. The worker then placed 500 ml of ethyl alcohol (concentration 55% weight) in the bottle. Thereby, the powder of vitamin E is immersed in the ethyl alcohol. The powder was immersed in the ethyl alcohol for 12 hours while sealed in the bottle. Meanwhile, the temperature of ethyl alcohol was 80 to 90 degrees Celsius. Meanwhile, the powder and the ethyl alcohol were stirred with a stirrer. Thereafter, the worker left the mixture for 12 hours. After 12 hours, a thin oil film was formed on the surface of the mixture. The oil film is purified vitamin E (hereinafter referred to as “purified vitamin E”). The worker accommodated 10 milligrams (20 milliliters) of this purified vitamin E in a predetermined tube. This tube is the same type of tube used for the preparation of melinjo extract tubes. The tube containing 10 milligrams of purified vitamin E is a vitamin tube.

(B) Preparation of sample The worker prepared purified melinjo extract solution (mass was 6 grams) contained in three meringo extract tubes and purified vitamin E (10 milligrams) contained in one vitamin tube. Were dissolved in 4.5 ml of dimethyl sulfoxide stock solution in a test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are as follows: gnetin C 1.37% by weight, resveratrol 0.44% by weight, dimethyl sulfoxide 60.46% by weight, vitamin E 2.22% by weight, moisture Is 35.51% by weight.

(C) Preparation of liquid medium First, the operator added the following seven kinds of substances to 1 liter of sterilized purified water (manufactured by ASONE CORPORATION). The first substance is 10 grams of powder medium (Wako Pure Chemical Industries, E-MEM Powder, product number 054-09001). The second substance is the serum of a newborn calf within 10 days after birth (product number 04-102-1A, manufactured by Cosmo Bio Inc.). The operator added 100 ml of this serum to the sterile purified water described above. The third substance is penicillin streptomycin (manufactured by Wako Pure Chemical Industries, Ltd., product number 168-23191). The amount of penicillin / streptomycin added was 10 ml. The fourth substance is a vitamin solution (Wako Pure Chemical Industries, Ltd., MEM vitamin solution, product number 130-17141). The amount of vitamin solution added was 10 ml. The fifth substance is a non-essential amino acid solution (Wako Pure Chemical Industries, Ltd., MEM non-essential amino acid solution, product number 139-15651). The amount of the non-essential amino acid solution added was 10 ml. The sixth substance is a sodium pyruvate solution (manufactured by Wako Pure Chemical Industries, Ltd., sodium pyruvate solution, product number 190-14881). The amount of this sodium pyruvate solution added was 10 ml. The seventh substance is 13.4 grams of sodium bicarbonate (Wako Pure Chemical Industries, Ltd., product number 198-01315). When these seven substances were added to the sterilized purified water described above, the operator agitated the sterilized purified water with the substances added. Stirred sterilized purified water is the liquid medium according to this example. In the following description, this liquid medium is referred to as “liquid medium”.

(D) Preparation of Collagen Gel Coat Preparation First, an operator spread 50 microliters of collagen medium (manufactured by Nippi Co., Ltd., collagen gel cell culture kit, product number 891503) on the prepared preparation. Once the collagen medium was spread, the operator placed the preparation in an incubator. The temperature inside the incubator was 37 degrees Celsius. Inside the incubator, the preparation was irradiated with ultraviolet rays having a wavelength of 365 nm. Thereby, the culture medium was dried. When the ultraviolet rays were irradiated for 2 hours, the worker removed the preparation from the incubator. This is a collagen gel coat preparation used for cell observation.

(E) Observation of cells First, the operator was cultivated in 20 microliters of liquid medium in a preparation of collagen gel-coated preparation. Two cells (ATCC number CRL-2936) were added together with the liquid medium. MDCK. After 60 minutes have passed since the two cells were placed in the preparation, the operator can then confirm the MDCK. Two cells were loaded with 20 microliters of the liquid described below. The liquid contained the influenza virus H3N2 (ATCC number VR-1680). As a result, the MDCK. Two cells were infected with H3N2. After 50 minutes had passed since the infection, the worker had MDCK. A sample of 20 microliters according to this example was applied to 2 cells. These operations were performed in an environment that can be observed with an optical microscope. After these operations are completed, the worker continues to use MDCK. Two cells were observed with a light microscope.

(2) Observation results MDCK. Two cells began to swell after infection with H3N2. Ten minutes after the sample was applied, some MDCK. Although 2 cells were killed, most MDCK. 2 cells began to adhere to the bottom of the preparation and return to normal morphology. About 50 minutes after the sample was applied, MDCK. Two cell divisions were initiated. 50 minutes after the sample was applied, MDCK. Since 2 cells returned to normal and started dividing, it is considered that H3N2 was killed.

[Example 2]
(1) Test procedure An operator shall prepare 13.5 ml of purified meringo extract solution contained in nine meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 18 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are 4.11% by weight of gnetin C, 1.33% by weight of resveratrol, 58.17% by weight of dimethyl sulfoxide, 2.22% by weight of vitamin E, moisture Is 34.17% by weight. Other points are the same as in the first embodiment.

(2) Observation results MDCK. Two cells began to swell after infection with H3N2. After the sample has been applied, almost all MDCK. Two cells returned to normal. Cell division started approximately 50 minutes after the sample was applied.

[Example 3]
(1) Test procedure The operator puts 4.5 ml of purified merinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture was doubled. The concentrations of gnetin C, resveratrol, and vitamin E were diluted by half. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are as follows: gnetin C is 0.69% by weight, resveratrol is 0.22% by weight, dimethyl sulfoxide is 61.72% by weight, vitamin E is 1.11% by weight, moisture Is 36.26% by weight. Other points are the same as in the first embodiment.

(2) Observation result The observation result was the same as that of Example 2.

[Example 4]
(1) Test procedure The operator puts 4.5 ml of purified merinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture became 4 times. The concentrations of gnetin C, resveratrol, and vitamin E were diluted by a factor of four. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to the present example were 0.34% by weight of gnetin C, 0.11% by weight of resveratrol, 62.36% by weight of dimethyl sulfoxide, 0.56% by weight of vitamin E, moisture Is 36.63 wt%. Other points are the same as in the first embodiment.

[Example 5]
(1) Test procedure The operator puts 4.5 ml of purified merinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture became 8 times. The concentrations of gnetin C, resveratrol, and vitamin E were diluted 1/8. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are 0.17% by weight of gnetin C, 0.06% by weight of resveratrol, 62.68% by weight of dimethyl sulfoxide, 0.28% by weight of vitamin E, moisture Is 36.81% by weight. Other points are the same as in the first embodiment.

[Example 6]
(1) Test procedure In preparation of the sample, the operator tested nine meringo extract tubes (18 grams of purified meringo extract solution) and purified vitamin E (10 milligrams) contained in one vitamin tube. It was dissolved in 13.5 ml dimethyl sulfoxide stock solution in a tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 18 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are 4.11% by weight of gnetin C, 1.33% by weight of resveratrol, 58.17% by weight of dimethyl sulfoxide, 2.22% by weight of vitamin E, moisture Is 34.17% by weight. In addition, when observing the cells, the operator placed MDCK. Two cells were added. This MDCK. Two cells were cultured in 20 microliters of liquid medium. This MDCK. Two cells were placed in the wells along with the liquid medium. MDCK. After 60 minutes have passed since the two cells were placed in the preparation, the operator can then confirm the MDCK. Two cells were loaded with 20 microliters of the liquid described below. The liquid contained RS virus (ATCC number VR-1580). As a result, the MDCK. Two cells were infected with RS virus. Other points are the same as in the first embodiment.

(2) Observation Results FIG. 1 shows MDCK. 2 When the cells were placed in the collagen gel coat preparation indentation, the MDCK. It is an optical micrograph of 2 cells. FIG. 2 shows MDCK. 2 When 70 minutes have passed since the cells were placed in the preparation of the collagen gel coat preparation (when 10 minutes have passed since infection with the RS virus), the MDCK. It is an optical micrograph of 2 cells. FIG. 3 shows MDCK. 2 When 100 minutes have passed since the cells were placed in the preparation of the collagen gel coat preparation (when 40 minutes have passed after infection with the RS virus), the MDCK. It is an optical micrograph of 2 cells. FIG. 4 shows MDCK. 2 When 120 minutes have passed since the cells were put into the preparation of the collagen gel coat preparation (60 minutes after infection with the RS virus), the MDCK. It is an optical micrograph of 2 cells. FIG. 5 shows MDCK. The MDCK.2 at the time when 150 minutes have passed since the 2 cells were placed in the preparation of the collagen gel coat preparation (90 minutes after infection with the RS virus). It is an optical micrograph of 2 cells. FIG. 6 is a block diagram of MDCK. It is the figure which showed the microscope picture of 2 cells along the time series. As shown in FIGS. 1 to 6, particularly FIGS. 1 and 2, MDCK. Two cells began to swell after infection with the RS virus. After the sample is applied, some MDCK. Although 2 cells were killed, most MDCK. Two cells returned to normal. Cell division started approximately 10 minutes after the sample was applied. Also, as shown in FIG. 5, when about 40 minutes had passed after the sample was applied, the expanded cells adhered to the bottom of the preparation, and the cell morphology returned to normal. The number of cells has not decreased. 10 minutes after the sample was applied, MDCK. Since the two cells start dividing and return to a normal form by 60 minutes, it is considered that the RS virus has been killed.

[Example 7]
(1) Test procedure The operator puts 4.5 ml of purified merinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are as follows: gnetin C 1.37% by weight, resveratrol 0.44% by weight, dimethyl sulfoxide 60.46% by weight, vitamin E 2.22% by weight, moisture Is 35.51% by weight. The other points are the same as in the sixth embodiment.

(2) Observation results MDCK. Two cells began to swell after infection with the RS virus. After the sample has been applied, almost all MDCK. Two cells returned to normal. Cell division started approximately 10 minutes after the sample was applied. About 60 minutes after the sample was applied, the number of cells increased and the morphology returned to normal, and it is considered that the RS virus had died.

[Example 8]
(1) Test procedure The operator puts 4.5 ml of purified merinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture was doubled. The concentrations of gnetin C, resveratrol, and vitamin E were diluted by half. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are as follows: gnetin C is 0.69% by weight, resveratrol is 0.22% by weight, dimethyl sulfoxide is 61.72% by weight, vitamin E is 1.11% by weight, moisture Is 36.26% by weight. The other points are the same as in the sixth embodiment.

(2) Observation result The observation result was the same as that of Example 7.

[Example 9]
The worker dissolves the purified meringo extract solution contained in three meringo extract tubes and the purified vitamin E contained in one vitamin tube in 4.5 ml dimethyl sulfoxide stock solution in a test tube. I let you. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture became 4 times. The concentrations of gnetin C, resveratrol and vitamin E were diluted by a factor of four. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to the present example were 0.34% by weight of gnetin C, 0.11% by weight of resveratrol, 62.36% by weight of dimethyl sulfoxide, 0.56% by weight of vitamin E, moisture Is 36.63 wt%. The other points are the same as in the sixth embodiment.

[Example 10]
The worker dissolves the purified meringo extract solution contained in three meringo extract tubes and the purified vitamin E contained in one vitamin tube in 4.5 ml dimethyl sulfoxide stock solution in a test tube. I let you. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture became 8 times. The concentrations of gnetin C, resveratrol, and vitamin E were diluted 1/8. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are 0.17% by weight of gnetin C, 0.06% by weight of resveratrol, 62.68% by weight of dimethyl sulfoxide, 0.28% by weight of vitamin E, moisture Is 36.81% by weight. The other points are the same as in the sixth embodiment.

[Example 11]
(1) Test Procedure (A) Preparation of Melinjo Extract Tube The worker prepared a melinjo extract tube according to this example according to the following procedure. First, the worker dipped the Merinjo fruit in 55% by weight ethyl alcohol in a sealed bottle for 12 hours. The meringo was made by pulverizing the whole skin, fruit and seeds. The meringo was a raw meringo that was not dry. The mass of merinjo fruit for one meringo extract tube was 50 grams. The volume of ethyl alcohol for one meringo extract tube was 500 ml. While the meringo was soaked, the temperature of the ethyl alcohol was maintained at 90 degrees Celsius. While the meringo was soaked, the ethyl alcohol continued to be stirred by the stirrer. After 12 hours of immersing the Merinjo in ethyl alcohol, the worker removed the Merinjo's solid components from the ethyl alcohol. Once the solid component was removed from the ethyl alcohol, the operator placed the ethyl alcohol in a sealed bottle. The operator caused the pump to discharge the gas in the bottle out of the bottle. At that time, the temperature inside the bottle was 40 degrees Celsius. As the gas was evacuated, the ethyl alcohol inside the bottle evaporated. The operator put 30 ml of the solid content remaining in the bottle into a predetermined tube. The tube containing this solid content is the melinjo extract tube in this example.

(B) Preparation of sample The worker prepared 4.5 ml of purified melinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. In dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are as follows: gnetin C 1.37% by weight, resveratrol 0.44% by weight, dimethyl sulfoxide 60.46% by weight, vitamin E 2.22% by weight, moisture Is 35.51% by weight.

(C) Culture of bacteria First, the worker formed a lump of cooked rice by grasping the cooked rice with his bare hands. When the cooked rice lump was formed, the worker left the cooked rice lump for a day. After the lump of cooked rice was left for a day, the operator took 5 g of the lump of cooked rice and mixed it with phosphate buffered saline PBS (diluted solution) containing pH 7.4 and 7.5 wt% sodium chloride. Crushed. The operator smeared the ground diluted solution onto the egg yolk-added mannitol salt medium. When the ground rice was smeared on the egg yolk-added mannitol salt medium, the worker left the egg yolk-added mannitol salt medium at 36 degrees Celsius for 48 hours. Thereby, bacterial colonies were formed. After the yolk-added mannitol salt medium was left for 48 hours, the worker dissolved the bacterial colonies formed in the egg yolk-added mannitol salt medium in 100 microliters of phosphate buffered saline. The phosphate buffered saline in which the bacterial colonies are dissolved is a bacterial sample according to this example.

(E) Observation of bacteria First, the operator put 20 microliters of phosphate buffered saline in the well of the preparation. When 20 microliters of phosphate buffered saline was placed in the well, the operator placed 20 microliters of bacterial sample in the well. Next, the worker applied the sample according to this example to the bacterial sample. Thereafter, the operator observed the bacteria in the recess with an optical microscope.

(2) Observation results The bacterial sample contained Staphylococcus aureus and Neisseria gonorrhoeae. These bacteria were sinking to the bottom of the preparation recess when one hour had passed since the sample according to this example was applied. All bacteria died 1 hour after the sample was applied.

[Example 12]
(1) Test procedure In this comparative example, the worker puts purified melinjo extract solution contained in nine meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. In 13.5 milliliters of dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 18 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this example. Therefore, the components of the sample according to this example are 4.11% by weight of gnetin C, 1.33% by weight of resveratrol, 2.22% by weight of vitamin E, 58.17% by weight of dimethyl sulfoxide, and moisture. Is 34.17% by weight. Other points in the sample preparation, the bacterial culture procedure, and the bacterial observation procedure are the same as in Example 11.

(2) Observation result The observation result was the same as that of Example 11.

[Comparative Example 1]
(1) Test procedure The operator made a purified melinjo extract solution (mass was 60 grams) contained in 30 meringo extract tubes and purified vitamin E (10 milligrams) contained in one vitamin tube. Were dissolved in 45 ml of dimethyl sulfoxide stock solution in a test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 60 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example were as follows: gnetin C 13.7% by weight, resveratrol 4.4% by weight, dimethyl sulfoxide 50.20% by weight, vitamin E 2.22% by weight, moisture Is 29.48% by weight. Other points are the same as in the first embodiment.

(2) Observation results MDCK. Two cells began to swell after infection with H3N2. MDCK. The cell division of the two cells stopped. After the sample is applied, MDCK. Two cells gradually decreased. MDCK. No cell division of 2 cells was observed.

[Comparative Example 2]
(1) Test procedure The operator puts 4.5 ml of purified merinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture became 16 times. The concentrations of gnetin C, resveratrol and vitamin E were diluted 1/16. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example were as follows: gnetin C 0.09% by weight, resveratrol 0.06% by weight, dimethyl sulfoxide 62.81% by weight, vitamin E 0.14% by weight, moisture Is 36.90% by weight. Other points are the same as in the first embodiment.

(2) Observation results MDCK. Two cells began to swell after infection with H3N2. After the sample is applied, MDCK. There was no change in the morphology of the two cells. MDCK. Two cells did not divide and the number of cells decreased after 60 minutes. Swelled infected cells were also seen.

[Comparative Example 3]
(1) Test procedure The worker dissolved the purified melinjo extract solution contained in the three melinjo extract tubes in 4.5 ml of dimethyl sulfoxide stock solution in the test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 1.37% by weight of gnetin C, 0.44% by weight of resveratrol, 61.86% by weight of dimethyl sulfoxide, and 36.33% by weight of water. Other points are the same as in the first embodiment.

(2) Observation Result The observation result was the same as Comparative Example 2.

[Comparative Example 4]
(1) Test procedure The worker dissolved purified vitamin E (10 milligrams) contained in one vitamin tube in 4.5 milliliters of dimethyl sulfoxide stock solution in a test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 2.22% by weight of vitamin E, 61.60% by weight of dimethyl sulfoxide, and 36.18% by weight of water. Other points are the same as in the first embodiment.
(2) Observation Result The observation result was the same as Comparative Example 2.

[Comparative Example 5]
(1) Test procedure (A) Preparation of catechin extract tube The operator prepared the catechin extract tube. The “catechin extract tube” is a tube in which a purified catechin extract solution described later is accommodated in a predetermined tube described later. The operator prepared the catechin extract tube according to this example according to the following procedure. First, the worker roasted leaves of Canoki (scientific name: Camellia sinensis (L.) Kuntze) at 110 degrees Celsius for 10 minutes. The weight of the leaves of one catechin extract tube was 50 grams. When the roasting was finished, the operator soaked the roasted chanoki leaves in 55% by weight ethyl alcohol in a sealed bottle for 24 hours. The volume of ethyl alcohol for one catechin extract tube was 500 ml. The temperature of the ethyl alcohol was maintained at 90 degrees Celsius while the leaves were pickled. The ethyl alcohol continued to be agitated by the stirrer while the leaf was pickled. After 24 hours of chanoki leaves were soaked in ethyl alcohol, the worker extracted unpurified catechin extract from the ethyl alcohol supernatant. A centrifugal evaporator (EC-57C manufactured by Sakuma Seisakusho Co., Ltd.) was used for extraction of the unpurified catechin extract. The extraction time was 12 hours. The unpurified catechin extract extracted by the centrifugal evaporator was a dry powder. When the crude catechin extract was extracted, the worker added an aqueous dimethyl sulfoxide solution (the concentration of dimethyl sulfoxide was 63% by weight) to the crude catechin extract and stirred. The amount of the aqueous dimethyl sulfoxide solution added was 3 ml with respect to 5 ml of the supernatant of ethyl alcohol from which the unpurified meringo extract was extracted. The unpurified catechin extract in which a dimethyl sulfoxide aqueous solution is added and stirred is the unpurified catechin extract solution in this example. When stirring was completed, the operator removed impurities from the unpurified catechin extract solution by using a centrifuge (Eppendorf Model 5415R). The rotational speed of this centrifuge was 15400 rpm. This centrifuge was used for 10 minutes. By removing the impurities, the unpurified Kitekin extract solution became a purified catechin extract solution. The operator accommodated 2 grams (30 milliliters) of this purified catechin extract solution in a predetermined tube. This is a catechin extract tube.

(B) Preparation of sample In this comparative example, the worker puts purified catechin extract solution contained in nine catechin extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in 4.5 ml dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are catechin 16.80% by weight, vitamin E 2.22% by weight, dimethyl sulfoxide 51.02%, and moisture 29.96% by weight. Other points are the same as in the first embodiment.

(2) Observation results MDCK. Two cells began to swell after infection with H3N2. Even after the sample is applied, MDCK. There was no improvement in the state of the two cells, and MDCK. Two cells died sequentially. MDCK. No cell division was observed in 2 cells, and swollen infected cells were also observed.

[Comparative Example 6]
(1) Test procedure In this comparative example, the operator dissolved the purified catechin extract solution contained in the three catechin extract tubes in 4.5 ml of a dimethyl sulfoxide stock solution in a test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 5.6% by weight of catechin, 2.22% by weight of vitamin E, 58.17% by weight of dimethyl sulfoxide, and 34.11% by weight of water. Other points are the same as in the first embodiment.

(2) Observation Result The observation result was the same as Comparative Example 5.

[Comparative Example 7]
(1) Test procedure In this comparative example, an operator dissolved purified vitamin E (10 milligrams) contained in one vitamin tube in 4.5 milliliters of a dimethyl sulfoxide stock solution in a test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 2.22% by weight of vitamin E, 61.6% by weight of dimethyl sulfoxide, and 36.18% by weight of water. Other points are the same as in the first embodiment.

[Comparative Example 8]
(1) Test procedure When preparing the sample, the operator tested 30 meringo extract tubes (60 grams of purified meringo extract solution) and purified vitamin E (10 milligrams) contained in one vitamin tube. It was dissolved in 45 ml of dimethyl sulfoxide stock solution in a tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 60 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example were as follows: gnetin C 13.7% by weight, resveratrol 4.4% by weight, dimethyl sulfoxide 50.20% by weight, vitamin E 2.22% by weight, moisture Is 29.48% by weight. The other points are the same as in the sixth embodiment.

(2) Observation results MDCK. Two cells began to swell after infection with the RS virus. MDCK. The cell division of the two cells stopped. After the sample is applied, MDCK. Two cells gradually decreased. MDCK. No cell division of 2 cells was observed.

[Comparative Example 9]
(1) Test procedure The operator puts 4.5 ml of purified merinjo extract solution contained in three meringo extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. Next, the worker added a dimethyl sulfoxide aqueous solution (the concentration of dimethyl sulfoxide was 63% by weight) to the mixed solution. Thereby, the volume of the liquid mixture became 16 times. The concentrations of gnetin C, resveratrol and vitamin E were diluted 1/16. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example were as follows: gnetin C 0.09% by weight, resveratrol 0.06% by weight, dimethyl sulfoxide 62.81% by weight, vitamin E 0.14% by weight, moisture Is 36.90% by weight. The other points are the same as in the sixth embodiment.

(2) Observation results MDCK. Two cells began to swell after infection with the RS virus. After the sample is applied, MDCK. There was no change in the morphology of the two cells. Swelled infected cells were also seen.

[Comparative Example 10]
(1) Test procedure The worker dissolved the purified melinjo extract solution contained in the three melinjo extract tubes in 4.5 ml of dimethyl sulfoxide stock solution in the test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 29 ml. The volume of purified water in this dimethyl sulfoxide aqueous solution was 10 ml. This mixed liquid is a sample according to this comparative example. The other points are the same as in the sixth embodiment. Therefore, the components of the sample according to this comparative example are 1.37% by weight of gnetin C, 0.44% by weight of resveratrol, 61.86% by weight of dimethyl sulfoxide, and 36.33% by weight of water.

(2) Observation results MDCK. Two cells began to swell after infection with the RS virus. MDCK. The cell division of the two cells stopped. After the sample was applied, a relatively large number of MDCK. 2 cells returned to normal, but some MDCK. 2-cell MDCK. Two cells remained expanded. MDCK. Two-cell cell division did not occur.

[Comparative Example 11]
(1) Test procedure The worker used 4.5 ml dimethyl sulfoxide in a test tube of purified catechin extract solution contained in three catechin extract tubes and purified vitamin E contained in one vitamin tube. Dissolved in the stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 5.6% by weight of catechin, 2.22% by weight of vitamin E, 58.07% by weight of dimethyl sulfoxide, and 34.11% by weight of water. The other points are the same as in the sixth embodiment.

(2) Observation Result The observation result was the same as Comparative Example 9.

[Comparative Example 12]
(1) Test procedure An operator uses 13.5 milliliters of dimethyl sulfoxide in a test tube with purified catechin extract solution contained in 9 catechin extract tubes and purified vitamin E contained in one vitamin tube. Dissolved in the stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 18 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are catechin 16.80% by weight, vitamin E 2.22% by weight, dimethyl sulfoxide 51.02% by weight, and moisture 29.96% by weight. The other points are the same as in the sixth embodiment.

[Comparative Example 13]
(1) Test procedure The sample of this comparative example is the same as that of the comparative example 7. The other points are the same as in the sixth embodiment.

[Comparative Example 14]
(1) Test Procedure In this comparative example, MDCK. Two cells were placed in a collagen gel coat preparation. MDCK. After 60 minutes have passed since the 2 cells were added to the preparation, the operator can then confirm the MDCK. Two cells were loaded with 20 microliters of the liquid described below. The liquid contained RS virus (ATCC number VR-1580). As a result, the MDCK. Two cells were infected with RS virus. Thereafter, the MDCK. Nothing was applied to the two cells. After the liquid containing the RS virus was applied, the worker continued to use MDCK. Two cells were observed with a light microscope.

(2) Observation Results FIG. 7 shows MDCK. 2 When the cells were placed in the collagen gel coat preparation indentation, the MDCK. It is an optical micrograph of 2 cells. FIG. 8 shows MDCK. 2 When 70 minutes have passed since the cells were placed in the preparation of the collagen gel coat preparation (when 10 minutes have passed since infection with the RS virus), the MDCK. It is an optical micrograph of 2 cells. FIG. 9 shows MDCK. 2 When 120 minutes have passed since the cells were put into the preparation of the collagen gel coat preparation (60 minutes after infection with the RS virus), the MDCK. It is an optical micrograph of 2 cells. FIG. 10 shows MDCK. 2 When the 180 cells have passed since the cells were placed in the preparation of the collagen gel coat preparation (120 minutes after infection with the RS virus), the MDCK. It is an optical micrograph of 2 cells. FIG. 11 shows MDCK. It is the figure which showed the microscope picture of 2 cells along the time series. As shown in FIGS. 7 to 11, MDCK. Two cells began to swell after infection with the RS virus. MDCK. The cell division of the two cells stopped.

[Comparative Example 15]
(1) Test procedure In this comparative example, an operator can prepare a purified catechin extract solution contained in nine catechin extract tubes and a purified vitamin E contained in one vitamin tube in a test tube. Dissolved in 5 ml dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 18 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are catechin 16.80% by weight, vitamin E 2.22% by weight, dimethyl sulfoxide 51.02% by weight, and moisture 29.96% by weight. Other points in the sample preparation, the bacterial culture procedure, and the bacterial observation procedure are the same as in Example 11.

(2) Observation Results Bacteria survived after 2 hours from the application of the sample and did not disappear. Most of Staphylococcus aureus weakened and died. The gonococcus was not killed and survived.

[Comparative Example 16]
(1) Test procedure In this comparative example, the worker puts purified catechin extract solution contained in three catechin extract tubes and purified vitamin E contained in one vitamin tube in a test tube. Dissolved in 5 ml dimethyl sulfoxide stock solution. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 5.6% by weight of catechin, 2.22% by weight of vitamin E, 58.07% by weight of dimethyl sulfoxide, and 34.11% by weight of water. Other points in the sample preparation, the bacterial culture procedure, and the bacterial observation procedure are the same as in Example 11.

(2) Observation Result The observation result was the same as that of Comparative Example 15.

[Comparative Example 17]
(1) Test procedure In this comparative example, an operator dissolved purified vitamin E (10 milligrams) contained in one vitamin tube in 4.5 milliliters of a dimethyl sulfoxide stock solution in a test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 2.22% by weight of vitamin E, 61.6% by weight of dimethyl sulfoxide, and 36.18% by weight of water. Other points in the sample preparation, the bacterial culture procedure, and the bacterial observation procedure are the same as in Example 11.

(2) Observation Results Bacteria did not show any change in the form or number of Staphylococcus aureus at the time when 2 hours had passed since the sample was applied.

[Comparative Example 18]
(1) Test procedure In this comparative example, the operator dissolved the purified catechin extract solution contained in the three catechin extract tubes in 4.5 ml of a dimethyl sulfoxide stock solution in a test tube. Thereafter, the worker added purified water to the mixed solution. The volume of the mixed solution to which purified water was added was 6 ml. The operator further added 45 ml of a dimethyl sulfoxide aqueous solution to the mixture. Of this aqueous dimethyl sulfoxide solution, the volume of dimethyl sulfoxide was 28 ml. The volume of purified water in this aqueous dimethyl sulfoxide solution was 17 ml. This mixed liquid is a sample according to this comparative example. Therefore, the components of the sample according to this comparative example are 5.6% by weight of catechin, 58.07% by weight of dimethyl sulfoxide, 2.22% by weight of vitamin E, and 34.11% by weight of water. Other points in the sample preparation, the bacterial culture procedure, and the bacterial observation procedure are the same as in Example 11.

[Comparative Example 19]
In this comparative example, the bacterial sample cultured in Example 11 was used. The operator applied only 20 microliters of phosphate buffered saline described in the description of Example 11 to the bacterial sample. Thereafter, the operator observed the bacterial sample. At 2 hours after the application of phosphate buffered saline, there was no change in the form or number of S. aureus.

Claims

Gnetin C,
With resveratrol,
Vitamin E,
An antibiotic containing a fluidizing substance,
The weight% of the gnetin C is 0.17 wt% or more and 4.11 wt% or less,
% By weight of the resveratrol is 0.06 wt% or more and 1.33 wt% or less,
An antibiotic having a vitamin E content of 0.28 wt% or more and 2.22 wt% or less.
The weight% of the gnetin C is 0.17 wt% or more and 1.37 wt% or less,
The antibiotic according to claim 1, wherein a weight percent of the resveratrol is 0.06 wt% or more and 0.44 wt% or less.
The antibiotic according to claim 2, wherein the sum of the weight% of the gnetin C and the weight% of the resveratrol is 0.23 wt% or more and 1.81 wt% or less.
The weight% of the gnetin C is 0.17 wt% or more and 0.69 wt% or less
The antibiotic according to claim 1, wherein a weight percent of the resveratrol is 0.06 wt% or more and 0.22 wt% or less.