WO2018018783A1 - 一种益生菌整理剂的制作方法 - Google Patents

一种益生菌整理剂的制作方法 Download PDF

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WO2018018783A1
WO2018018783A1 PCT/CN2016/105674 CN2016105674W WO2018018783A1 WO 2018018783 A1 WO2018018783 A1 WO 2018018783A1 CN 2016105674 W CN2016105674 W CN 2016105674W WO 2018018783 A1 WO2018018783 A1 WO 2018018783A1
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probiotics
probiotic
microcapsule
finishing agent
microcapsules
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French (fr)
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汪明星
刘金抗
陈永兵
陈凤
孙利萍
葛玲
王雪芬
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紫罗兰家纺科技股份有限公司
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • A61K35/741—Probiotics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/4808—Preparations in capsules, e.g. of gelatin, of chocolate characterised by the form of the capsule or the structure of the filling; Capsules containing small tablets; Capsules with outer layer for immediate drug release
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/10—Laxatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
    • A61P1/12—Antidiarrhoeals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/02—Drugs for genital or sexual disorders; Contraceptives for disorders of the vagina
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/08—Antiallergic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • D—TEXTILES; PAPER
    • D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
    • D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
    • D06M23/00—Treatment of fibres, threads, yarns, fabrics or fibrous goods made from such materials, characterised by the process
    • D06M23/12—Processes in which the treating agent is incorporated in microcapsules

Definitions

  • the invention particularly relates to a method for preparing a probiotic finishing agent.
  • Probiotics are a kind of active microorganisms beneficial to the host. They are the general term for active beneficial microorganisms that are colonized in the human intestines and reproductive system and can produce exact health effects to improve the balance of the micro-ecology of the host and play a beneficial role.
  • the beneficial bacteria or fungi in the human body and animal body mainly include: Clostridium butyricum, lactic acid bacteria, bifidobacteria, Lactobacillus acidophilus, actinomycetes, yeasts and the like.
  • the most powerful products studied in the world are mainly composite active probiotics composed of the above various microorganisms, which are widely used in bioengineering, industry and agriculture, food safety and life health.
  • the probiotics that scientists have discovered can be broadly divided into three categories, including: 1 Lactobacillus (such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus johnsonii, Lactobacillus ramisii, etc.); 2 Bifidobacteria (such as Bifidobacterium longum, Bifidobacterium breve, Bifidobacterium ovale, Bifidobacterium thermophilus, etc.; 3 Gram-positive cocci (such as Streptococcus faecalis, Lactococcus, Intermediate Streptococcus, etc.). In addition, some yeasts and enzymes can also be classified as probiotics.
  • 1 Lactobacillus such as Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus johnsonii, Lactobacillus ramisii, etc.
  • Bifidobacteria Such as Bifidobacterium long
  • Lactobacillus can help the body break down lactose, relieve diarrhea, flatulence and other symptoms, can be eaten with milk.
  • probiotics can regulate excessive or excessive immunological activity to a normal state by stimulating immune function in the intestine.
  • the role of probiotics in this immunomodulation is also believed to contribute to anti-cancer and allergic diseases.
  • probiotics can inhibit the proliferation of harmful bacteria in the intestine, reduce toxins, promote intestinal peristalsis, thereby improving intestinal function and improving defecation.
  • Lactobacillus acidophilus is a representative strain in this respect, which secretes lactase which digests lactose, thereby alleviating lactose intolerance.
  • Probiotics produce vitamins, including pantothenic acid, niacin, B1, B2, B6 and vitamin K. They also produce short-chain fatty acids, antioxidants, amino acids, etc., which play an important role in bone growth and heart health.
  • probiotics have these benefits, they are easy to inactivate and need to be used orally in use. If they can be used as a finishing agent on the surface of people's contact, people can supplement probiotics during their daily life and use them.
  • Probiotics The colonization process inhibits harmful and harmful microbes and will be another green health revolution that will change human health.
  • the present invention provides a method for preparing a probiotics finishing agent, which comprises a method for preparing a probiotic bacteria by dormancy and microcapsule encapsulation, and can be grafted on a surface of a living environment, furniture, textiles, etc.
  • Probiotic microcapsules enable people to infect probiotics in their lives to maintain probiotics on the human body and in the body.
  • a method for preparing a probiotic finishing agent comprising the following steps:
  • Probiotics culture is industrialized direct-fermentation fermentation, cultivating 3 genera and 11 species of probiotic bacteria recognized by the state, accounting for 143 strains, and 11 species of probiotics are all kinds of strains that can be used in the health food industry, and Ensure the strain activity of the strain;
  • Preliminary freeze-concentration of active probiotics concentration by centrifugation at 0 °C - 4 °C or direct drying of probiotics at -1 °C - 50 °C to make the probiotics per gram of 1-50 billion strains, adding microcapsule wall material
  • the oily wall material is used for probiotic microcapsule encapsulation: after evenly mixing the wall material and the bacterial liquid, the lyophilized powder technology is used for vacuum drying, and the completely dried lyophilized material is obtained.
  • the airflow is pulverized to 3000-5000 mesh in a low temperature -1 ° C - 50 ° C environment to obtain a probiotic fungal low temperature dormant microcapsule;
  • each microcapsule is a separate separator.
  • the internal environment of the microcapsules is relatively dry and the probiotics are relatively dormant until the microcapsules are outside the ring.
  • the probiotics in the microcapsules of the suitable growth species will "infect" the outside world, and the active species will still be stored inside the microcapsules;
  • the preparation of the microcapsule finishing agent is carried out by using the microcapsule lyophilized powder and the nano-adhesive material according to a certain ratio at room temperature, specifically: adding 3-5 kg of nano microcapsule lyophilized powder to the nano resin containing 20-100 nm resin 20% The liquid is mixed with 97-95 kg to obtain 3-6% probiotics containing microcapsules. When used, it is diluted to 2-5% as needed to be used in household products and clothing.
  • the wall material is one or more of the following materials: a natural polymer material, a semi-synthetic polymer material, and a fully synthetic polymer material.
  • the natural polymer materials are: gelatin, gum arabic, shellac, shellac, starch, dextrin, wax, turpentine, sodium alginate, corn mash, chitosan.
  • the semi-synthetic polymer material is carboxymethyl cellulose, methyl cellulose, ethyl cellulose.
  • the fully synthetic polymer material is: polyethylene, polystyrene, polybutadiene, polypropylene, polyether, polyurea, polyethylene glycol, polyvinyl alcohol, polyamide, polyacrylamide, polyurethane , polymethyl methacrylate, polyvinyl pyrrolidone, epoxy resin, polysiloxane.
  • the present invention is a method for preparing a probiotic by dormancy and microcapsule encapsulation treatment, and can graft dormant probiotic microcapsules on the surface of a living environment, furniture, textiles, etc., so that people in life Probiotics can be used to probiotics on the human body and in the body, avoiding the inconvenience of oral use and the persistence of probiotic survival.
  • probiotics first multiply, while inhibiting harmful bacteria and harmful microorganisms, and in the human body.
  • the external environment forms a probiotic-protected community.
  • a method for preparing a probiotic finishing agent comprises the following steps:
  • Probiotics culture is industrialized direct-fermentation fermentation, cultivating 3 genera and 11 species of probiotic bacteria recognized by the state, accounting for 143 strains, and 11 species of probiotics are all kinds of strains that can be used in the health food industry, and Ensure the strain activity of the strain.
  • Preliminary freeze-concentration of active probiotics concentration by centrifugation at 0 °C - 4 °C or direct drying of probiotics at -1 °C - 50 °C to make the probiotics per gram of 1-50 billion strains, adding microcapsule wall material
  • the oily wall material is used for probiotic microcapsule encapsulation: after evenly mixing the wall material and the bacterial liquid, the lyophilized powder technology is used for vacuum drying, and the completely dried lyophilized material is obtained.
  • the airflow is pulverized to 3000-5000 mesh at a low temperature of -1 ° C - 50 ° C to obtain a low-temperature dormant microcapsule of probiotic bacteria.
  • the wall material is one or more of the following materials: a natural polymer material, a semi-synthetic polymer material, and a fully synthetic polymer material.
  • the natural polymer materials are: gelatin, gum arabic, shellac, shellac, starch, dextrin, wax, turpentine, sodium alginate, corn mash, chitosan.
  • the semi-synthetic polymer material is carboxymethyl cellulose, methyl cellulose, or ethyl cellulose.
  • the fully synthetic polymer material is: polyethylene, polystyrene, polybutadiene, polypropylene, polyether, polyurea, polyethylene glycol, polyvinyl alcohol, polyamide, polyacrylamide, polyurethane, polymethyl Methyl acrylate, polyvinyl pyrrolidone, epoxy resin, polysiloxane.
  • each microcapsule is a separate isolation
  • the internal environment of the microcapsules is relatively dry and the probiotics are in a relatively dormant state until the microcapsules are suitable for the growth of the bacteria.
  • the probiotics in the microcapsules will grow and expand to "infect" the outside world, while the microcapsules still store active species inside.
  • the preparation of the microcapsule finishing agent is carried out by using the microcapsule lyophilized powder and the nano-adhesive material according to a certain ratio at room temperature, specifically: adding 3-5 kg of nano microcapsule lyophilized powder to the nano resin containing 20-100 nm resin 20% The liquid is mixed with 97-95 kg to obtain 3-6% probiotics containing microcapsules. When used, it is diluted to 2-5% as needed to be used in household products and clothing.
  • the invention is a method for preparing a probiotic by dormancy and microcapsule encapsulation, and can graft the dormant microcapsules on the surface of the living environment, furniture, textiles, etc., so that people can be inoculated in the life.
  • the bacteria maintain probiotics on the human body and in the body, avoiding the inconvenience of oral use and the persistence of probiotic survival. As long as it is suitable for bacterial growth, the probiotics first multiply, while inhibiting harmful bacteria and harmful microorganisms, and forming in the human environment. Probiotics protect the community.

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Abstract

一种益生菌整理剂的制作方法，包括如下步骤：益生菌培养；益生菌微胶囊包裹；益生菌休眠；微胶囊的附着剂调和。是将益生菌进行休眠和微胶囊包裹处理做成整理剂的方法，可以在人们生活环境的墙面、家具、纺织品等表面嫁接休眠的益生菌微胶囊，使人在生活中得以接种益生菌对人体表和体内做益生菌养护，避免了口服使用的不便性和益生菌存活的持久性，只要适合细菌生长，益生菌首先繁殖，同时抑制有害菌和有害微生物，并且在人体外环境形成益生菌保护群落。

Description

一种益生菌整理剂的制作方法 技术领域
本发明具体涉及一种益生菌整理剂的制作方法。
背景技术
益生菌是一类对宿主有益的活性微生物，是定植于人体肠道、生殖系统内，能产生确切健康功效从而改善宿主微生态平衡、发挥有益作用的活性有益微生物的总称。人体、动物体内有益的细菌或真菌主要有：酪酸梭菌、乳酸菌、双歧杆菌、嗜酸乳杆菌、放线菌、酵母菌等。目前世界上研究的功能最强大的产品主要是以上各类微生物组成的复合活性益生菌，其广泛应用于生物工程、工农业、食品安全以及生命健康领域。科学家已发现的益生菌大体上可分成三大类，其中包括：①乳杆菌类(如嗜酸乳杆菌、干酪乳杆菌、詹氏乳杆菌、拉曼乳杆菌等)；②双歧杆菌类(如长双歧杆菌、短双歧杆菌、卵形双歧杆菌、嗜热双歧杆菌等)；③革兰氏阳性球菌(如粪链球菌、乳球菌、中介链球菌等)。此外，还有一些酵母菌与酶亦可归入益生菌的范畴。
当人体住满足够的益生菌时，人就会处于健康的状态，但是一旦体内菌群失去平衡，比如菌种间比例发生大幅变化或者超出正常数值时，那么腹泻、过敏、胃口不佳、疲倦、免疫力低等一系列病症就会随之而来，人体的健康，而这时适当添加益生菌，协助体内菌群平衡，才能让人重现健康状态。国外一些学者认为，其作用可以概括为：
1、预防或改善腹泻：饮食习惯不良或服用抗生素均会打破肠道菌群平衡，从而导致腹泻。补充益生菌有助于平衡肠道菌群及恢复正常的肠道pH值，缓解腹泻症状。
2、缓解不耐乳糖症状：乳杆菌可帮助人体分解乳糖，缓解腹泻、胀气等不适症状，可与牛奶同食。
3、预防生殖系统感染：酸牛奶、瑞卡福抑菌喷剂中的嗜酸乳杆菌可抑制阴道内白色念珠菌的繁殖。欧洲所做的盲对照试验证实了这一点。患者每天给体内补充10ml活性益生菌，结果显示生殖系统感染病发病率大大降低，长期给体内补充活性益生菌能够帮助修复生殖系统微循环。
4、增强人体免疫力：在肠道内存在着非常发达的免疫系统。益生菌可以通过刺激肠道内的免疫机能，将过低或过高的免疫活性调节至正常状态。益生菌这种免疫调节的作用也被认为有助于抗癌与抑制过敏性疾病。
5、促进肠道消化系统健康：益生菌可以抑制有害菌在肠内的繁殖，减少毒素，促进肠道蠕动，从而提高肠道机能，改善排便状况。
6、降低血清胆固醇：欧洲的高加索山区、地中海沿岸是著名的长寿之乡，当地人常饮自制的酸牛奶，极少患糖尿病、心血管病及肥胖症，大量科学研究证实这与酸牛奶中富含益生菌有关。这些益生菌可降低血清胆固醇水平，此外，长期补充益生菌的还有助于防止骨质丢失，预防骨质疏松症。
7、帮助吸收营养成分：如果每天摄入益生菌，不仅能够扼制肠内有害菌群的产生，还能为肠内有益菌提供良好的生长环境，造就健康肠道。
病人经常服用含益生菌的保健食品，可预防与治疗腹泻症。正常人体肠道内栖息着500多种、数十万亿个不同的细菌，它们在绝大多数情况下是互相制约、共存共荣的。一旦肠道菌丛平衡被打破，就会引起腹泻。其次，滥用抗生素也会引起腹泻。欧洲一些医疗中心试用以乳杆菌、双歧杆菌与菊糖为主要成分的口服液治疗旅行者腹泻，也取得如下良好效果：
1.帮助营养物质的消化吸收
许多益生菌株在胃肠道内可产生消化酶，这些酶可帮助人体更好地消化所摄入的食品及吸收食品中的营养成分。益生菌还可竞争性抑制有害微生物吸收营养物质及进入血液循环系统。嗜酸乳杆菌是这方面的代表菌株，可分泌消化乳糖的乳糖酶，从而缓解乳糖不耐症。
2.产生重要的营养物质
益生菌能产生维生素，包括泛酸、尼克酸、B1,B2,B6及维生素K等，同时能产生短链脂肪酸、抗氧化剂、氨基酸等等，对骨骼成长和心脏健康有重要作用。
3.抵抗细菌病毒的感染，提升免疫通过三大步骤，益生菌可清除有害菌对身体的伤害：
1.)抑制有害菌的生长。益生菌通过产生杀灭有害菌的化学物质，及与有害菌竞争空间和资源而遏制它们的生长。
2.)抑制有害菌产生毒素。
3.)清除有害菌产生的毒素。
4.预防和治疗某些疾病，如肠道综合症、呼吸道感染、生殖系统感染、过敏、口臭、胃溃疡等。
人体许多健康问题都是由体内菌群失衡引起，决定因素是微生态的平衡，可通过使体内菌群重新达到生态平衡来实现缓解与治疗的目的。比如腹泻、便秘、阴道感染等综合症状，国内外已经经过多年的研究和实践，证明使用特定的经临床证实的益生菌可有效地进行治疗。益生菌虽然有这些好处，但是在使用中存在容易失活、需要口服使用等；如果可以在制成整理剂形式作用在人们接触的物品表面，让人们在生活起居时候可以补充益生菌，并且利用益生菌繁 殖过程抑制有害菌和有害微生物那么将是改变人类健康生活的另一种绿色健康革命。
发明内容
发明目的：本发明提供一种益生菌整理剂的制作方法，将益生菌进行休眠和微胶囊包裹处理做成整理剂的方法，可以在人们生活环境的墙面、家具、纺织品等表面嫁接休眠的益生菌微胶囊，使人在生活中得以接种益生菌对人体表和体内做益生菌养护。
技术方案：一种益生菌整理剂的制作方法，包括如下步骤：
(1)、益生菌培养
益生菌培养为工业化直投发酵，培养国家认可的益生菌菌种3个属、11个种,计143株,所收录的益生菌11个种均为可用于保健食品行业生产的菌种，并且保证菌种的菌株活性；
(2)、益生菌微胶囊包裹
初步冷冻浓缩活性益生菌：采用0℃-4℃离心脱水浓缩或者直接在-1℃—-50℃冷冻真空干燥益生菌原液使每克益生菌含量在1-500亿株，加入微胶囊壁材，为了使益生菌休眠效果好选用油性壁材进行益生菌微胶囊包裹：搅拌均匀使壁材和菌液均匀混合后，用冻干粉技术进行冷冻真空干燥，得到完全干燥冻干料后再在低温-1℃—-50℃环境进行气流粉碎到3000-5000目，得到益生菌低温休眠微胶囊；
(3)、益生菌休眠
由于益生菌被微胶囊包裹同时是油性包裹，每个微胶囊是一个单独的隔离体，微胶囊内部环境相对很干燥益生菌处于相对休眠状态，直至微胶囊外界环 境适宜菌种生长微胶囊内益生菌才会对外生长扩张“感染”外界，而微胶囊内部依然储存活性菌种；
(4)、微胶囊的附着剂调和
微胶囊整理剂的配制，采用微胶囊冻干粉和纳米粘合材料按照一定比例常温混合，具体为：用3-5公斤纳米微胶囊冻干粉加入含有30-100纳米树脂20％的纳米树脂液体97-95公斤混合得到含微胶囊3-6％的益生菌整理剂，使用时根据需要稀释到2-5％在家居产品和服装上整理使用。
作为优化：所述壁材是以下材料中的一种或几种：天然高分子材料、半合成高分子材料、全合成高分子材料。
作为优化：所述天然高分子材料为：明胶、阿拉伯胶、虫胶、紫胶、淀粉、糊精、蜡、松脂、海藻酸钠、玉米朊、壳聚糖。
作为优化：所述半合成高分子材料为：羧甲基纤维素、甲基纤维素、乙基纤维素。
作为优化：所述全合成高分子材料为：聚乙烯、聚苯乙烯、聚丁二烯、聚丙烯、聚醚、聚脲、聚乙二醇、聚乙烯醇、聚酰胺、聚丙烯酰胺、聚氨酯、聚甲基丙烯酸甲酯、聚乙烯吡咯烷酮、环氧树脂、聚硅氧烷。
有益效果：本发明是将益生菌进行休眠和微胶囊包裹处理做成整理剂的方法，可以在人们生活环境的墙面、家具、纺织品等表面嫁接休眠的益生菌微胶囊，使人在生活中得以接种益生菌对人体表和体内做益生菌养护，避免了口服使用的不便性和益生菌存活的持久性，只要适合细菌生长，益生菌首先繁殖，同时抑制有害菌和有害微生物，并且在人体外环境形成益生菌保护群落。
具体实施方式
下面结合具体实施例对本发明进行详细阐述。
具体实施例一：
一种益生菌整理剂的制作方法，包括如下步骤：
(1)、益生菌培养
益生菌培养为工业化直投发酵，培养国家认可的益生菌菌种3个属、11个种,计143株,所收录的益生菌11个种均为可用于保健食品行业生产的菌种，并且保证菌种的菌株活性。
(2)、益生菌微胶囊包裹
初步冷冻浓缩活性益生菌：采用0℃-4℃离心脱水浓缩或者直接在-1℃—-50℃冷冻真空干燥益生菌原液使每克益生菌含量在1-500亿株，加入微胶囊壁材，为了使益生菌休眠效果好选用油性壁材进行益生菌微胶囊包裹：搅拌均匀使壁材和菌液均匀混合后，用冻干粉技术进行冷冻真空干燥，得到完全干燥冻干料后再在低温-1℃—-50℃环境进行气流粉碎到3000-5000目，得到益生菌低温休眠微胶囊。
所述壁材是以下材料中的一种或几种：天然高分子材料、半合成高分子材料、全合成高分子材料。所述天然高分子材料为：明胶、阿拉伯胶、虫胶、紫胶、淀粉、糊精、蜡、松脂、海藻酸钠、玉米朊、壳聚糖。所述半合成高分子材料为：羧甲基纤维素、甲基纤维素、乙基纤维素。所述全合成高分子材料为：聚乙烯、聚苯乙烯、聚丁二烯、聚丙烯、聚醚、聚脲、聚乙二醇、聚乙烯醇、聚酰胺、聚丙烯酰胺、聚氨酯、聚甲基丙烯酸甲酯、聚乙烯吡咯烷酮、环氧树脂、聚硅氧烷。
(3)、益生菌休眠
由于益生菌被微胶囊包裹同时是油性包裹，每个微胶囊是一个单独的隔离 体，微胶囊内部环境相对很干燥益生菌处于相对休眠状态，直至微胶囊外界环境适宜菌种生长微胶囊内益生菌才会对外生长扩张“感染”外界，而微胶囊内部依然储存活性菌种。
(4)、微胶囊的附着剂调和
微胶囊整理剂的配制，采用微胶囊冻干粉和纳米粘合材料按照一定比例常温混合，具体为：用3-5公斤纳米微胶囊冻干粉加入含有30-100纳米树脂20％的纳米树脂液体97-95公斤混合得到含微胶囊3-6％的益生菌整理剂，使用时根据需要稀释到2-5％在家居产品和服装上整理使用。
本发明是将益生菌进行休眠和微胶囊包裹处理做成整理剂的方法，可以在人们生活环境的墙面、家具、纺织品等表面嫁接休眠的益生菌微胶囊，使人在生活中得以接种益生菌对人体表和体内做益生菌养护，避免了口服使用的不便性和益生菌存活的持久性，只要适合细菌生长，益生菌首先繁殖，同时抑制有害菌和有害微生物，并且在人体外环境形成益生菌保护群落。
本发明不局限于上述最佳实施方式，任何人在本发明的启示下都可得出其他各种形式的产品，但不论在其形状或结构上作任何变化，凡是具有与本申请相同或相近似的技术方案，均落在本发明的保护范围之内。

Claims (5)

  1. 一种益生菌整理剂的制作方法，其特征在于：包括如下步骤：
    (1)、益生菌培养
    益生菌培养为工业化直投发酵，培养国家认可的益生菌菌种3个属、11个种,计143株,所收录的益生菌11个种均为可用于保健食品行业生产的菌种，并且保证菌种的菌株活性；
    (2)、益生菌微胶囊包裹
    初步冷冻浓缩活性益生菌：采用0℃-4℃离心脱水浓缩或者直接在-1℃—-50℃冷冻真空干燥益生菌原液使每克益生菌含量在1-500亿株，加入微胶囊壁材，
    为了使益生菌休眠效果好选用油性壁材进行益生菌微胶囊包裹：搅拌均匀使壁材和菌液均匀混合后，用冻干粉技术进行冷冻真空干燥，得到完全干燥冻干料后再在低温-1℃—-50℃环境进行气流粉碎到3000-5000目，得到益生菌低温休眠微胶囊；
    (3)、益生菌休眠
    由于益生菌被微胶囊包裹同时是油性包裹，每个微胶囊是一个单独的隔离体，微胶囊内部环境相对很干燥益生菌处于相对休眠状态，直至微胶囊外界环境适宜菌种生长微胶囊内益生菌才会对外生长扩张“感染”外界，而微胶囊内部依然储存活性菌种；
    (4)、微胶囊的附着剂调和
    微胶囊整理剂的配制，采用微胶囊冻干粉和纳米粘合材料按照一定比例常温混合，具体为：用3-5公斤纳米微胶囊冻干粉加入含有30-100纳米树脂20％的纳米树脂液体97-95公斤混合得到含微胶囊3-6％的益生菌整理剂，使用时根据需要稀释到2-5％在家居产品和服装上整理使用。
  2. 根据权利要求1所述的益生菌整理剂的制作方法，其特征在于：所述壁材是以下材料中的一种或几种：天然高分子材料、半合成高分子材料、全合成高分子材料。
  3. 根据权利要求2所述的益生菌整理剂的制作方法，其特征在于：所述天然高分子材料为：明胶、阿拉伯胶、虫胶、紫胶、淀粉、糊精、蜡、松脂、海藻酸钠、玉米朊、壳聚糖。
  4. 根据权利要求2所述的益生菌整理剂的制作方法，其特征在于：所述半合成高分子材料为：羧甲基纤维素、甲基纤维素、乙基纤维素。
  5. 根据权利要求2所述的益生菌整理剂的制作方法，其特征在于：所述全合成高分子材料为：聚乙烯、聚苯乙烯、聚丁二烯、聚丙烯、聚醚、聚脲、聚乙二醇、聚乙烯醇、聚酰胺、聚丙烯酰胺、聚氨酯、聚甲基丙烯酸甲酯、聚乙烯吡咯烷酮、环氧树脂、聚硅氧烷。
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