WO2017204442A3 - 비대칭 입자를 이용한 표적 물질 검출 키트 및 방법 - Google Patents

비대칭 입자를 이용한 표적 물질 검출 키트 및 방법 Download PDF

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Publication number
WO2017204442A3
WO2017204442A3 PCT/KR2017/002592 KR2017002592W WO2017204442A3 WO 2017204442 A3 WO2017204442 A3 WO 2017204442A3 KR 2017002592 W KR2017002592 W KR 2017002592W WO 2017204442 A3 WO2017204442 A3 WO 2017204442A3
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kit
beads
asymmetric
marker substance
detecting marker
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PCT/KR2017/002592
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English (en)
French (fr)
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WO2017204442A2 (ko
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이정훈
한상훈
김성구
베르마네하
이예진
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서울대학교 산학협력단
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Publication of WO2017204442A2 publication Critical patent/WO2017204442A2/ko
Publication of WO2017204442A3 publication Critical patent/WO2017204442A3/ko

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G01N33/54333—Modification of conditions of immunological binding reaction, e.g. use of more than one type of particle, use of chemical agents to improve binding, choice of incubation time or application of magnetic field during binding reaction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54346—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
    • G01N33/545—Synthetic resin

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  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nanotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

본 발명은 크기가 상이한 입자의 비대칭 응집을 이용한 표적 물질 검출 키트 및 표적 물질의 검출 방법에 관한 것으로, 표적 물질에 대하여 서로 다른 결합 분자를 사용하면 입자 간의 비대칭 복합체를 얻을 수 있으며, 이를 분석하여 불특정 응집 효과를 배제하고, 높은 특이적 신호를 확보할 수 있어 각종 표적 물질을 효율적으로 검출할 수 있다.
PCT/KR2017/002592 2016-05-26 2017-03-09 비대칭 입자를 이용한 표적 물질 검출 키트 및 방법 WO2017204442A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2016-0065067 2016-05-26
KR20160065067 2016-05-26

Publications (2)

Publication Number Publication Date
WO2017204442A2 WO2017204442A2 (ko) 2017-11-30
WO2017204442A3 true WO2017204442A3 (ko) 2018-08-02

Family

ID=60412502

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Application Number Title Priority Date Filing Date
PCT/KR2017/002592 WO2017204442A2 (ko) 2016-05-26 2017-03-09 비대칭 입자를 이용한 표적 물질 검출 키트 및 방법

Country Status (2)

Country Link
KR (1) KR102026521B1 (ko)
WO (1) WO2017204442A2 (ko)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111044724B (zh) * 2019-12-22 2023-11-10 复星诊断科技(上海)有限公司 一种胸苷激酶1磁微粒化学发光测定试剂盒及其制备方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060133596A (ko) * 2004-01-30 2006-12-26 큐티엘 바이오시스템즈 엘엘씨 생물학적 및 화학적 시료의 검출 방법
WO2009048878A2 (en) * 2007-10-09 2009-04-16 University Of Notre Dame Du Lac Microfluidic platforms for multi-target detection
KR20130123766A (ko) * 2012-05-03 2013-11-13 한국과학기술연구원 금 나노입자를 이용하여 신호 증폭도가 향상된, 표적 물질의 다중화 처리 분석법과 그 시스템
US20130337455A1 (en) * 2012-05-03 2013-12-19 The Regents Of The University Of Michigan Multi-Mode Separation for Target Detection and Cell Growth Monitoring
US20140243231A1 (en) * 2013-02-28 2014-08-28 The Terasaki Family Foundation Methods and compositions for the efficient reuse of multiply dyed particles

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007003412A (ja) * 2005-06-24 2007-01-11 Sekisui Chem Co Ltd 生物学的測定方法
US20120288852A1 (en) * 2010-01-15 2012-11-15 Richard Willson Force Mediated Assays
CN103025859A (zh) 2010-05-25 2013-04-03 阿尔利克斯公司 用于检测生物学和化学分析中的颗粒的位置自由度的方法和装置以及在免疫诊断学中的应用
KR101277696B1 (ko) * 2011-05-06 2013-06-24 한국식품연구원 타겟팅-금 자성 나노입자―기반된 미생물의 원-스텝 분리, 농축 및 검출 방법
CN104471380B (zh) 2012-07-06 2017-11-17 株式会社日立高新技术 分析装置以及分析方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20060133596A (ko) * 2004-01-30 2006-12-26 큐티엘 바이오시스템즈 엘엘씨 생물학적 및 화학적 시료의 검출 방법
WO2009048878A2 (en) * 2007-10-09 2009-04-16 University Of Notre Dame Du Lac Microfluidic platforms for multi-target detection
KR20130123766A (ko) * 2012-05-03 2013-11-13 한국과학기술연구원 금 나노입자를 이용하여 신호 증폭도가 향상된, 표적 물질의 다중화 처리 분석법과 그 시스템
US20130337455A1 (en) * 2012-05-03 2013-12-19 The Regents Of The University Of Michigan Multi-Mode Separation for Target Detection and Cell Growth Monitoring
US20140243231A1 (en) * 2013-02-28 2014-08-28 The Terasaki Family Foundation Methods and compositions for the efficient reuse of multiply dyed particles

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Publication number Publication date
KR102026521B1 (ko) 2019-09-30
KR20170134188A (ko) 2017-12-06
WO2017204442A2 (ko) 2017-11-30

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