WO2017185558A1 - 含有噻苯隆和超敏蛋白的植物生长调节剂组合物 - Google Patents

含有噻苯隆和超敏蛋白的植物生长调节剂组合物 Download PDF

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WO2017185558A1
WO2017185558A1 PCT/CN2016/094034 CN2016094034W WO2017185558A1 WO 2017185558 A1 WO2017185558 A1 WO 2017185558A1 CN 2016094034 W CN2016094034 W CN 2016094034W WO 2017185558 A1 WO2017185558 A1 WO 2017185558A1
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protein
active component
composition
plant growth
hypersensitive
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PCT/CN2016/094034
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English (en)
French (fr)
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仲汉根
吴进龙
季红进
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江苏辉丰农化股份有限公司
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Publication of WO2017185558A1 publication Critical patent/WO2017185558A1/zh

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/28—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N<
    • A01N47/36—Ureas or thioureas containing the groups >N—CO—N< or >N—CS—N< containing the group >N—CO—N< directly attached to at least one heterocyclic ring; Thio analogues thereof

Definitions

  • the invention belongs to the field of agricultural plant protection, in particular to a plant growth regulator composition with improved properties, in particular to a composition comprising a thidiazuron and a hypersensitive protein pesticide.
  • the harpin protein is a special protein that can induce plants to produce defensive responses and enhance plant growth and development. Because of its unique mechanism of action, it has improved immunity and enhanced resistance to diseases and insect pests in crops, fruit trees and flowers. Regulate growth and development, improve crop quality, increase production and income. It is an agricultural biotechnology product developed by protein elicitors produced by plant pathogenic bacteria. The dosage form is 3% microparticles. It was invented in 1992 by Dr. Wei Zhongmin of Cornell University. Registered in the United States by Eden Biotech in the United States in 2000
  • Pyraclostrobin is a new type of high-efficiency plant growth regulator. When it is absorbed by plants, it can promote the separation of petioles and stems and form natural shedding. It can be widely used in pesticides and fertilizers. It has strong biological activity, and its ability to induce plant cell division and callus formation is many times higher than that of general cytokinins. Improve crop absorptive capacity and increase photosynthesis, so as to increase production and enhance the purpose. It can be used in various kinds of melons, fruits, vegetables, seedlings, flowers, food, tobacco and other crops, and can also be used for tissue culture, genetic engineering, etc. In production, it can significantly improve crop yield, improve quality and use less, has no pollution to the ecological environment, and is non-toxic to humans, animals, birds, bees and natural enemies.
  • the plant growth regulators currently on the market are mainly based on a single agent, and the effect is single.
  • the inventors have found that there is a significant synergistic effect between the hypersensitive protein and the thidiazuron in regulating or promoting plant growth.
  • Another object of the present invention is to provide a process for the preparation of a composition comprising two active ingredients A and B plant growth regulators and for regulating and promoting the growth of crops in the agricultural field.
  • the object of the invention can be achieved by the following measures:
  • a synergistic plant growth regulator composition comprising two active components A and B, wherein active component A is thidiazem and active component B is hypersensitive protein, two components The weight ratio between the active components A The weight ratio to the active ingredient B is 1:0.05 to 90.
  • the weight ratio of the active component A to the active component B is 1:0.05-85.
  • the weight ratio of the active component A to the active component B is 1: 0.05 to 80.
  • the weight ratio of active ingredient A to active ingredient B is from 1:0.1 to 85.
  • the weight ratio of active component A to active component B is from 1:1 to 80.
  • the weight ratio of active ingredient A to active ingredient B is from 1:10 to 80.
  • the weight ratio between the two components of A and B can be arbitrarily adjusted within the range of the following ratios: 1:0.05, 1:0.051, 1:0.052, 1:0.055, 1:0.054 , 1:0.055, 1:0.056, 1:0.057, 1:0.058, 1:0.059, 1:0.06, 1:0.07, 1:0.08, 1:0.09, 1:0.1, 1:0.11, 1:0.12,1 : 0.13, 1:0.14, 1:0.15, 1:0.16, 1:0.17, 1:0.18, 1:0.19, 1:0.2, 1:0.3, 1:0.4, 1:0.5, 1:0.6, 1:0.7 , 1:0.8, 1:0.9, 1:1, 1:2, 1:3, 1:4, 1:5, 1:6, 1:7, 1:8, 1:9, 1:10, 1 :11, 1:12, 1:13, 1:14, 1:15, 1:16, 1:17, 1:18, 1:19, 1:20, 1:25, 1:30, 1:35 , 1:40, 1:45, 1:50, 1:55, 1
  • composition of the present invention is useful for better regulating the growth of crops, promoting the health effects of crops, increasing crop yields, and improving crop quality.
  • the invention provides the use of a composition comprising component A (thiabendazole) and component B (supersensitive protein) to regulate crop growth in the agricultural field, in particular to regulate crop growth in rice, corn or fruit trees to improve disease resistance Other uses.
  • component A thiabendazole
  • component B supersensitive protein
  • composition of the present invention can also be used in combination with other pesticides (such as fungicides, insecticides, herbicides, plant growth regulators), fertilizers, etc., and these are mixed with Commonly used pesticides or fertilizers disclosed in the art.
  • pesticides such as fungicides, insecticides, herbicides, plant growth regulators
  • fertilizers etc.
  • the composition of the present invention can be processed into a preparation of various dosage forms with a fertilizer, or co-administered or mixed with a fertilizer.
  • Suitable fertilizers include: inorganic fertilizers or organic fertilizers, such as one or more of a large number of elements containing nitrogen, phosphorus, potassium, etc., or containing copper, iron, manganese, zinc, boron, calcium, magnesium, sulfur, etc.
  • inorganic fertilizers or organic fertilizers such as one or more of a large number of elements containing nitrogen, phosphorus, potassium, etc., or containing copper, iron, manganese, zinc, boron, calcium, magnesium, sulfur, etc.
  • One or several trace elements in the mixture or a mixture containing at least one of humic acid, amino acid, chitin and the like.
  • composition of the present invention can be made into a pesticide-acceptable dosage form from the active ingredient and the pesticide adjuvant.
  • composition may specifically comprise an agrochemical adjuvant, such as one or more of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, a binder, a surfactant or a fertilizer, in the course of application.
  • an agrochemical adjuvant such as one or more of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, a binder, a surfactant or a fertilizer, in the course of application.
  • agrochemical adjuvant such as one or more of a carrier, a solvent, a dispersing agent, a wetting agent, an adhesive, a thickener, a binder, a surfactant or a fertilizer, in the course of application.
  • Common additives can be mixed in.
  • Suitable auxiliaries may be solid or liquid, they are usually materials commonly used in the processing of dosage forms, such as natural or regenerated minerals, solvents, dispersants, wetting agents, adhesives, thickeners, binders or fertilizers. .
  • the method of application of the compositions of the invention comprises the use of the compositions of the invention for aerial parts of plants, in particular leaves or foliage. You can choose to soak or apply to the surface of the control object. The frequency of application and the amount applied will depend on the growth of the crop.
  • compositions may be applied only with the active ingredient or may be mixed with an additive (agrochemical adjuvant).
  • composition of the present invention can be prepared into a pesticide-acceptable dosage form such as a liquid, a suspension, a soluble liquid, an oil suspension, a microcapsule, a microemulsion, a wettable powder and the like.
  • a pesticide-acceptable dosage form such as a liquid, a suspension, a soluble liquid, an oil suspension, a microcapsule, a microemulsion, a wettable powder and the like.
  • the compositions may be applied by spraying, misting, dusting, spreading or pouring, and the like.
  • Microcapsule dosage forms suitable for the present invention include: microcapsule suspension, microcapsule suspension-suspension, microcapsule suspension-water emulsion, etc., characterized in that the inner wall of the capsule (ie, the capsule core) contains the supersensitive protein and the thiobenzene. Long in accordance with a certain proportion of the mixture, that is, the two are mixed into a microcapsule.
  • the microcapsules are stably dispersed and suspended in a certain concentration to form a microcapsule suspending agent in water as a continuous phase.
  • one of the active ingredients is prepared as a microcapsule suspension, another active ingredient is prepared into an emulsion, and the prepared two components are mixed, stirred, and shaken to obtain a microcapsule suspension-suspension or microcapsule suspension.
  • Agent-water emulsion is a microcapsule suspension.
  • composition of the present invention can be prepared into various dosage forms by a known method, and the active ingredient and the auxiliary agent, such as a solvent, a solid carrier, and, if necessary, can be uniformly mixed and ground together with the surfactant to prepare a desired preparation. Dosage form.
  • the above solvent may be selected from aromatic hydrocarbons, preferably containing from 8 to 12 carbon atoms, such as a mixture of xylenes or substituted benzenes, phthalates such as dibutyl phthalate or dicaprylic acid, aliphatic hydrocarbons such as rings.
  • aromatic hydrocarbons preferably containing from 8 to 12 carbon atoms, such as a mixture of xylenes or substituted benzenes, phthalates such as dibutyl phthalate or dicaprylic acid, aliphatic hydrocarbons such as rings.
  • Alkenes or paraffins, alcohols and glycols and their ethers and esters such as ethanol, ethylene glycol, ethylene glycol monomethyl
  • ketones such as cyclohexanone
  • highly polar solvents such as N-methyl-2 Pyrrolidone, dimethyl sulfoxide or dimethylformamide
  • vegetable or vegetable oils such as soybean oil.
  • the above solid carriers are typically natural mineral fillers such as talc, kaolin, montmorillonite or activated clay.
  • a highly dispersible silicic acid or a highly dispersible adsorbent polymer carrier such as a particulate adsorbent carrier or a non-adsorbing carrier, and a suitable particulate adsorbent carrier is porous, such as pumice, bentonite or Bentonite; a suitable non-adsorbing carrier such as calcite or sand.
  • a large amount of pre-granulated materials of inorganic or organic nature can be used as a carrier, in particular dolomite.
  • Suitable surfactants are lignosulfonic acid, naphthalenesulfonic acid, phenolsulfonic acid, alkaline earth metal or amine salts, alkylarylsulfonates, alkyl groups Sulfate, alkyl sulfonate, fat Alcohol sulfate, fatty acid and sulfated fatty alcohol glycol ether, condensate of sulfonated naphthalene and naphthalene derivatives with formaldehyde, condensate of naphthalene or naphthalenesulfonic acid with phenol and formaldehyde, polyoxyethylene octylphenyl Ether, ethoxylated isooctyl phenol, octyl phenol, nonyl phenol, alkyl aryl polyglycol ether, tributyl benzene polyglycol ether
  • the mass content of the active ingredient is from 1 to 80%, preferably from 1 to 50%, more preferably from 2 to 30%.
  • Plants of the invention include, but are not limited to, strawberries or cherries.
  • the two active ingredients in the compositions of the present invention exhibit synergistic effects, the activity of which is more pronounced than the expected sum of activity using a single compound, and the individual activity of a single compound.
  • the other characteristics exhibited by the composition of the present invention are mainly as follows: 1.
  • the composition of the present invention has obvious synergistic effect; 2.
  • the composition of the invention can well regulate crop growth and improve disease resistance. Resistance, promote crop health, improve crop quality, and increase crop yield.
  • the active ingredient hypersensitive protein and the thidiazepine, and the components such as dispersant, wetting agent, thickener and water are uniformly mixed according to the formula, and after the sanding and/or high-speed shearing, the semi-finished product is obtained and analyzed. After the addition of water and evenly filtered, the finished product is obtained.
  • Example 2 30.6% thiabendazole ⁇ supersensitive protein suspension
  • Example 3 5.25% thiabendazole ⁇ supersensitive protein suspension
  • the active ingredient thiablon and the hypersensitive protein are mixed in a certain ratio, that is, the two are mixed into a microcapsule.
  • the microcapsules are stably dispersed and suspended in a certain concentration to form a microcapsule suspending agent in water as a continuous phase.
  • one of the active ingredients is prepared as a microcapsule suspension, another active ingredient is prepared into an emulsion, and the prepared two components are mixed, stirred, and shaken to obtain a microcapsule suspension-suspension or microcapsule suspension.
  • Agent-water emulsion Agent-water emulsion.
  • Example 4 14.2% thiabendazole ⁇ supersensitive protein microcapsule suspension
  • Thiabendazole 0.2% hypersensitive protein 14%, turpentine vegetable oil 12%, glyceryl lactate 4%, emulsifier polyoxyethylene castor oil 3%, stabilizer triphenyl phosphite 3.5%, toluene diisocyanate 15%, acrylic acid - 5% acrylate copolymer, 3% dipropylene glycol, 1% preservative sodium benzoate, 2.1% thickener xanthan gum, water up to 100%.
  • Example 5 24.8% thiabendazole ⁇ supersensitive protein microcapsule suspension-suspending agent
  • Example 6 2.2% thiabendazole ⁇ supersensitive protein microcapsule suspension-water emulsion
  • the active ingredient thiablon and the hypersensitive protein are placed in a kettle according to a certain proportion of the mixture, the surfactant, the auxiliary agent and the filler, and the mixture is thoroughly stirred to obtain a soluble powder.
  • Example 7 9.1% thidianon ⁇ supersensitive protein soluble powder
  • Example 8 18.3% thiabendazole ⁇ supersensitive protein soluble powder
  • Example 9 2% thiabendazole ⁇ supersensitive protein soluble powder
  • Example 10 20.2% thiabendazole ⁇ supersensitive protein aqueous solution
  • hypersensitive protein 20% Thiabendazole 0.2%, hypersensitive protein 20%, ethanol 5% mixed, stirred for 20 minutes, then added hypersensitive protein 28%, stirred for 30 minutes until the hypersensitive protein is completely dissolved; add 8% alkyl alcohol sodium sulfate, The amount of water is used to make up.
  • Example 11 2.1% thibenone-supersensitive protein aqueous solution
  • Example 12 10.2% thibenone-supersensitive protein aqueous solution
  • the active ingredient hypersensitive protein and the thidiazepine are mixed with the solvent, the emulsifier, the dispersing agent, the antifreezing agent and the water in the proportion of the formulation, and the mixture is thoroughly stirred to obtain an aqueous emulsion.
  • Example 14 20.5% thiabendazole ⁇ supersensitive protein water emulsion
  • Thiabendazole 0.5% hypersensitive protein 20%, xylene 10%, polyvinyl alcohol 5%, glycerol 5%, silicone defoamer 1%, water to 100%.
  • Thiabendazole 0.2% hypersensitive protein 2%, sodium dodecylbenzenesulfonate 10%, methanol 10%, polyvinyl alcohol 5%, glycerol 5%, silicone defoamer 1%, water complement To 100%.
  • Thiabendron 1% hypersensitive protein 0.3%, alkylphenol ethoxylate 10%, toluene 10%, glycerol 5%, silicone defoamer 1%, water to 100%.
  • the thidiazepine and the hypersensitive protein are diluted with acetone at a certain concentration, and the crops are sprayed with stems and leaves, and each is measured in the garden.
  • concentration on the growth of strawberry fruit, including the evaluation of strawberry petiole, single fruit weight, disease resistance, fruit quality, and yield.
  • Tip length increase rate (length of petiole after treatment - length of petiole of clear water control) / length of clear water control petiole * 100%
  • Fruit setting rate (the fruit setting rate after treatment - the fruit setting rate of the clear water control) / the water fruit comparison fruit setting rate * 100%
  • Single fruit weight increase rate (single fruit weight after treatment - single fruit weight of clear water control) / single fruit weight of clear water control * 100%
  • the experimental data from Table 2 shows that the product of the present invention is applied at the early flowering stage of the cherry, and can effectively regulate the healthy growth of the cherry.
  • the ratio of thiampone to hypersensitive protein is 1:(0.05-90)
  • the main performances are as follows: 1. It can significantly increase the fruit setting rate and increase the yield of cherry; 2. It can regulate the autoimmune resistance of cherry plants.
  • the invention can increase the individual weight of cherry; 4, the composition of the invention can increase the sugar content of the cherry, reduce the acidity of the cherry, the ratio of sugar to acid Significantly improved, can promote the improvement of cherry mouthfeel; 5, Example 10, Example 12, Example 16, ie, thidiazepine: the ratio of hypersensitive protein is less than 1:90 or greater than 1:0.05, relative to the single In contrast to the agent control and other examples, the synergistic effect was not significant and showed an additive effect.

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
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Abstract

提供了一种含有噻苯隆和超敏蛋白的植物生长调节剂组合物，其有效成分包含A和B，其中活性组分A为噻苯隆，活性组分B为超敏蛋白。还提供了该组合物的制备方法及用途。试验结果表明，该组合物可很好地调节作物生长，提高抗病抗逆性，促进作物健康作用，改善作物品质，提高作物产量。

Description

含有噻苯隆和超敏蛋白的植物生长调节剂组合物 技术领域
本发明属于农业植物保护领域，特别是涉及一种具有改进性能的植物生长调节剂组合物，具体地说是涉及一种包含噻苯隆和超敏蛋白农药的组合物。
背景技术
超敏蛋白(harpin protein)是一种能诱导植物产生防卫反应，增强植物生长发育功能的特殊蛋白质，因其独特的作用机理对农作物、果林、花卉等具有提高免疫力、增强抗病虫性和调节生长发育、改善作物品质、增产增收功能。是由植物病原细菌产生的蛋白激发子开发成的一种农用生物技术产品，剂型为3％微粒剂，它是美国康乃尔大学韦忠民博士在1992年研究发明的。2000年由美国伊甸生物技术公司在美国获得登记
噻苯隆(pyraclostrobin)是新型高效植物生长调节剂，噻苯隆被植物吸收后，可促进叶柄与茎之间的分离组织，形成自然脱落，在农药、肥料中可被广泛应用。它具有极强的生物活性，其诱导植物细胞分裂、愈伤组织形成的能力比一般细胞分裂素高许多倍。提高作物吸收能力，增加光合作用，从而达到增产、增优目的。可用于各类瓜、果、蔬菜、苗木、花卉、粮食、烟草等作物，还可用于组织培养、基因工程等。在生产上能显著提高作物产量、改善品质且有用量少、对生态环境无污染，对人、畜、鸟、蜜蜂、天敌无毒等特点。
目前市面上销售的植物生长调节剂主要是以单剂为主，且作用效果单一。
发明内容
本发明的目的是提供一种植物生长调节剂，以促进作物生长，提高作物的抗病、作物产量和改善作物的品质。发明人发现,超敏蛋白与噻苯隆两者之间在调节或促进植物生长方面具有显著的协同提升作用。
本发明的另一个目的是提供包含两种有效成分A和B植物生长调节剂组合物的制备方法及在农业领域调节和促进农作物生长的应用。
本发明的目的可以通过以下措施达到：
一种具有增效作用的植物生长调节剂组合物，该组合物包含A和B两种活性组分，其中活性组分A为噻苯隆，活性组分B为超敏蛋白，两组分之间的重量比为活性组分A 和活性组分B的重量比为1：0.05～90。
在一种优选方案中，活性组分A和活性组分B的重量比为1：0.05～85，为进一步提高协同作用的效果，活性组分A和活性组分B的重量比为为1：0.05～80。
在一种优选方案中，活性组分A和活性组分B的重量比为1：0.1～85。
在一种优选方案中，活性组分A和活性组分B的重量比为1：1～80。
在一种优选方案中，活性组分A和活性组分B的重量比为1：10～80。
一种优选的方案中，A和B两组分之间的重量比可以任意地在下述配比的范围内进行调整：1：0.05、1：0.051、1：0.052、1：0.055、1：0.054、1：0.055、1：0.056、1：0.057、1：0.058、1：0.059、1：0.06、1：0.07、1：0.08、1：0.09、1：0.1、1：0.11、1：0.12、1：0.13、1：0.14、1：0.15、1：0.16、1：0.17、1：0.18、1：0.19、1：0.2、1：0.3、1：0.4、1：0.5、1：0.6、1：0.7、1：0.8、1：0.9、1：1、1：2、1：3、1：4、1：5、1：6、1：7、1：8、1：9、1：10、1：11、1：12、1：13、1：14、1：15、1：16、1：17、1：18、1：19、1：20、1：25、1：30、1：35、1：40、1：45、1：50、1：55、1：60、1：65、1：70、1：75、1：80、1：85、1：90，也可以在以上任意两个配比所组成的范围内进行选择。
发明人通过试验发现，本发明的组合物是用于可以更好的调节作物的生长，促进作物的健康作用，提高作物的产量、改善作物品质。
本发明提供了包含组分A(噻苯隆)和组分B(超敏蛋白)的组合物在农业领域调节农作物生长的用途，特别是在水稻、玉米或果树方面调节作物生长提高抗病效果等方面的用途。
为充分发挥本发明的优势效果，本发明的组合物还可以与其他农药(如杀菌剂、杀虫剂、除草剂、植物生长调节剂)、肥料等混合使用，这些与之混用的均为现有技术公开的常用的农药或肥料。
为提高使用效果，本发明的组合物可以与肥料加工成各种剂型的制剂，或与肥料共同施用或混用。合适的肥料包括：无机肥或有机肥，具体的肥料如含有氮、磷、钾等中的一种或几种大量元素，或含有铜、铁、锰、锌、硼、钙、镁、硫等中的一种或几种微量元素，或含有腐殖酸、氨基酸、甲壳素等肥料的至少一种的混合物。
本发明的组合物可以由活性成分和农药助剂制成农药上允许的剂型。
上述组合物具体可包含农药助剂，例如载体、溶剂、分散剂、润湿剂、胶粘剂、增稠剂、粘合剂、表面活性剂或肥料等中的一种或几种，在施用的过程中可以混合常用的助剂。
合适的助剂可以是固体或液体，它们通常是剂型加工过程中常用的物质，例如天然的或再生的矿物质，溶剂、分散剂、润湿剂、胶粘剂、增稠剂、粘合剂或肥料。
本发明组合物的施用方法包括将本发明的组合物用于植物生长的地上部分，特别是叶部或叶面。可以选择浸种或涂抹于防治对象表面。施用的频率和施用量取决于作物的生长情况。
这些组合物可以仅仅包含活性成分进行施用，也可以与添加剂(农药助剂)一起混合使用。
本发明的组合物可以制备成农药上可接受的剂型，例如水剂、悬浮剂、可溶性液剂、油悬浮剂、微囊剂型、微乳剂、可湿性粉剂等。根据这些组合物的性质以及施用组合物所要达到的目的和环境情况，可以选择将组合物以喷雾、弥雾、喷粉、撒播或泼浇等之类的方法施用。
适合本发明的微囊剂型包括：微囊悬浮剂、微囊悬浮-悬浮剂、微囊悬浮-水乳剂等，其特征在于囊壁内(即囊芯)包含由有效成分超敏蛋白和噻苯隆按照一定比例的混合物，即将两者混合制成微型胶囊。将微型胶囊以一定的浓度稳定地分散、悬浮在作为连续相的水中形成微囊悬浮剂。或者将其中一种有效成分制备成微囊悬浮剂，将另外一种有效成分制备成乳液，将制备的两种成分在混合，搅拌，摇匀即得微囊悬浮剂-悬浮剂或微囊悬浮剂-水乳剂。
可用已知的方法可以将本发明的组合物制备成各种剂型，可以将有效成分与助剂，如溶剂、固体载体，需要时可以与表面活性剂一起均匀混合、研磨，制备成所需要的剂型。
上述的溶剂可选自芳香烃，优选含8-12个碳原子，如二甲苯混合物或取代的苯，酞酸酯类，如酞酸二丁酯或酞酸二辛酸，脂肪烃类，如环已烷或石蜡，醇和乙二醇和它们的醚和酯，如乙醇，乙二醇，乙二醇单甲基；酮类，如环已酮，强极性的溶剂，如N-甲基-2-吡咯烷酮，二甲基亚砜或二甲基甲酰胺，和植物油或植物油，如大豆油。
上述的固体载体，如用于可湿性粉剂和可分散剂的通常是天然矿物填料，例如滑石、高岭土，蒙脱石或活性白土。为了管理组合物的物理性能，也可以加入高分散性硅酸或高分散性吸附聚合物载体，例如粒状吸附载体或非吸附载体，合适的粒状吸附载体是多孔型的，如浮石、皂土或膨润土；合适的非吸附载体如方解石或砂。另外，可以使用大量的无机性质或有机性质的预制成粒状的材料作为载体，特别是白云石。
根据本发明的组合物中的有效成分的化学性质，合适的表面活性剂为木质素磺酸、萘磺酸、苯酚磺酸、碱土金属盐或胺盐，烷基芳基磺酸盐，烷基硫酸盐，烷基磺酸盐，脂肪 醇硫酸盐，脂肪酸和硫酸化脂肪醇乙二醇醚，还有磺化萘和萘衍生物与甲醛的缩合物，萘或萘磺酸与苯酚和甲醛的缩合物，聚氧乙烯辛基苯基醚，乙氧基化异辛基酚，辛基酚，壬基酚，烷基芳基聚乙二醇醚，三丁基苯聚乙二醇醚，三硬脂基苯基聚乙二醇醚，烷基芳基聚醚醇，乙氧基化蓖麻油，聚氧乙烯烷基醚，氧化乙烯缩合物、乙氧基化聚氧丙烯，月桂酸聚乙二醇醚缩醛，山梨醇酯，木质素亚硫酸盐废液和甲基纤维素。
本发明的组合物中，活性成分的质量含量为1～80％，优选1～50％，进一步优选2～30％。
本发明中的植物包括但不限于草莓或樱桃。
本发明的组合物中两种有效成分表现为增效效果，该组合物的活性比使用单个化合物的活性预期总和，以及单个化合物的单独活性更为显著。本发明的组合物的表现出的其它特点主要表现为：1、本发明的组合物混配具有明显的协同增效作用；2、本发明的组合物可很好的调节作物生长，提高抗病抗逆性，促进作物健康作用，改善作物品质，提高作物产量。
具体实施方式
为了使本发明的目的、技术方案及优点更加清楚明白，以下结合实施例，对本发明进行进一步详细说明。应当理解，此处所描述的具体实施例仅用以解释本发明，并不用于限定本发明，凡在本发明的精神和原则之内所做的任何修改、等同替换和改进等，均应包含在本发明的保护范围之内。
以下实施例所有配方中百分比均为重量百分比(折百)。本发明组合物各种制剂的加工工艺均为现有技术，根据不同情况可以有所变化。
一、剂型制备实施例
(一)悬浮剂的加工及实施例
将活性成分超敏蛋白与噻苯隆，与分散剂、润湿剂、增稠剂和水等各组分按配方的比例混合均匀，经砂磨和/或高速剪切后，得到半成品，分析后补加水混合均匀过滤即得成品。
实施例1：20.25％噻苯隆·超敏蛋白悬浮剂
噻苯隆0.25％，超敏蛋白20％，黄原胶4％，丙三醇2％，高岭土5％，硅酸铝镁5％，木质素磺酸钠5％,水补足至100％。
实施例2：30.6％噻苯隆·超敏蛋白悬浮剂
噻苯隆0.6％，超敏蛋白30％，膨润土7％，乙二醇3％，甲基萘磺酸钠甲醛缩合物5％， 水补足至100％。
实施例3：5.25％噻苯隆·超敏蛋白悬浮剂
噻苯隆5％，超敏蛋白0.25％，白炭黑5％，丙三醇6％，苯甲酸钠3％，脂肪醇聚氧乙烯醚磷酸酯5％,水补足至100％。
(二)微囊剂的加工及实施例
将有效成分噻苯隆与超敏蛋白按照一定比例的混合物，即将两者混合制成微型胶囊。将微型胶囊以一定的浓度稳定地分散、悬浮在作为连续相的水中形成微囊悬浮剂。或者将其中一种有效成分制备成微囊悬浮剂，将另外一种有效成分制备成乳液，将制备的两种成分在混合，搅拌，摇匀即得微囊悬浮剂-悬浮剂或微囊悬浮剂-水乳剂。
实施例4：14.2％噻苯隆·超敏蛋白微囊悬浮剂
噻苯隆0.2％，超敏蛋白14％，松脂植物油12％，乳酸甘油酯4％，乳化剂聚氧乙烯蓖麻油3％，稳定剂亚磷酸三苯酯3.5％，甲苯二异氰酸酯15％，丙烯酸-丙烯酸酯共聚物5％，二丙二醇3％，防腐剂苯甲酸钠1％，增稠剂黄原胶2.1％，水补足至100％。
实施例5:24.8％噻苯隆·超敏蛋白微囊悬浮-悬浮剂
噻苯隆0.8％，超敏蛋白24％，甲酯椰子油15％，聚氧乙烯单棕榈酸酯5％，BHT5％、引诱剂氨基酸2％，苯甲酸钠5％，乙二醇5％，乙酸乙烯乳液4％，黄原胶2％，水补足至100％。
实施例6:2.2％噻苯隆·超敏蛋白微囊悬浮-水乳剂
噻苯隆2％，超敏蛋白0.2％，明胶15％，乙醇4％，，乙二胺5％，甲基萘磺酸钠甲醛缩合物5％，水补足至100％。
(三)可溶性粉剂的加工及实施例
将有效成分噻苯隆与超敏蛋白按照一定比例的混合物、表面活性剂、助剂、填料，置于釜中，充分搅拌，即得可溶性粉剂。
实施例7:9.1％噻苯隆·超敏蛋白可溶性粉剂
噻苯隆0.1％，超敏蛋白9％，烷基苯磺酸钠5％，高岭土补足至100％。
实施例8:18.3％噻苯隆·超敏蛋白可溶性粉剂
噻苯隆0.3％，超敏蛋白18％，烷基酚聚氧乙烯醚聚氧丙烯醚2％，膨润土补足至100％
实施例9:2％噻苯隆·超敏蛋白可溶性粉剂
噻苯隆1％，超敏蛋白1％，苄基二甲基酚聚氧乙烯醚4％，硫酸铵盐补足至100％。
(四)水剂的加工及实施例
在反应釜中加入有效成分和助剂，余量用水补足，并搅拌均匀，即可得到噻苯隆·超 敏蛋白水剂。
实施例10:20.2％噻苯隆·超敏蛋白水剂
噻苯隆0.2％，超敏蛋白20％，乙醇5％混匀，搅拌20分钟，再加入超敏蛋白28％，搅拌30分钟至超敏蛋白完全溶解；加入8％烷基醇硫酸钠，余量用水补足。
实施例11:2.1％噻苯隆·超敏蛋白水剂
在反应釜中加入噻苯隆0.1％和乙醇8％，搅拌20分钟；再加入超敏蛋白2％，搅拌30分钟至超敏蛋白完全溶解；加入10％脂肪醇聚氧乙烯醚，余量用水补足。
实施例12:10.2％噻苯隆·超敏蛋白水剂
在反应釜中加入噻苯隆0.2％和乙醇5％，搅拌20分钟；再加入超敏蛋白10％，搅拌30分钟至超敏蛋白完全溶解；加入5％脂肪醇聚氧乙烯醚，余量用水补足。
(五)水乳剂的加工及实施例
将活性成分超敏蛋白与噻苯隆，与溶剂、乳化剂、分散剂、抗冻剂和水等各组分按配方的比例混合置于反应釜中，充分搅拌，即可得到水乳剂。
实施例13:12.9％噻苯隆·超敏蛋白水乳剂
噻苯隆0.15％，超敏蛋白12.75％，十二烷基苯磺酸钠10％，甲苯10％，，聚乙烯醇5％，丙三醇5％，有机硅消泡剂1％，水补足至100％。
实施例14:20.5％噻苯隆·超敏蛋白水乳剂
噻苯隆0.5％，超敏蛋白20％，二甲苯10％，聚乙烯醇5％，丙三醇5％，有机硅消泡剂1％，水补足至100％。
实施例15:2.2％噻苯隆·超敏蛋白水乳剂
噻苯隆0.2％，超敏蛋白2％，十二烷基苯磺酸钠10％，甲醇10％，，聚乙烯醇5％，丙三醇5％，有机硅消泡剂1％，水补足至100％。
实施例16:1.3％噻苯隆·超敏蛋白水乳剂
噻苯隆1％，超敏蛋白0.3％，烷基酚聚氧乙烯醚10％，甲苯10％，丙三醇5％，有机硅消泡剂1％，水补足至100％。
二、药效验证试验
(一)生物测定实施例
1、噻苯隆与超敏蛋白复配对草莓生长调节综合测定试验
将噻苯隆与超敏蛋白用丙酮按照一定浓度稀释，对作物进行茎叶喷雾，在园间测定各 个浓度对草莓果实生长的影响，包括对草莓的叶柄、单果重、抗病情况、果实品质，以及产量情况等进行综合评价。
叶柄长度提高率＝(处理后叶柄长度-清水对照的叶柄长度)/清水对照叶柄长度*100％
座果提高率＝(处理后座果率-清水对照的座果率)/清水对照座果率*100％
预防效果＝(清水对照的发病指数-处理后的发病指数)/清水对照的发病指数*100％
单果重提高率＝(处理后的单果重-清水对照的单果重)/清水对照的单果重*100％
含糖提高率＝(处理后的含糖量-清水对照的含糖量)/清水对照的亩产量*100％
表1.噻苯隆与超敏蛋白复配对草莓生长调节综合测定汇总表
Figure PCTCN2016094034-appb-000001
注：表格中“超”为“超敏蛋白”缩写，“噻”为“噻苯隆”的缩写。施药时期：始花期初期，该阶段草莓未发生开始发生白粉病。施药次数：1次；施药方式：茎叶喷雾；调查时期：草莓成熟期；本试验中，叶柄长度、发病指数、单果重、含量量等指标均为各处理的平均值。
通过实验发明人发现，噻苯隆与超敏蛋白之间具有很好协同提升作用，噻苯隆与超敏 蛋白混配在不同配比下，相对噻苯隆、超敏蛋白单剂，以及清水对照，对草莓的生长调节作用明显，主要表现在：1、本发明可有效增加草莓的叶柄长度；2、噻苯隆在作为单剂使用时对植物生长具有一定的抗病抗逆作用，而超敏蛋白是一种高生物活性物质，对作物病害具有一定免疫力，超敏蛋白与噻苯隆之间共同施用具有良好的协同作用，从上表分析可以看出，噻苯隆与超敏蛋白之间协同作用对草莓白粉病的预防效果相对于单剂及清水对照，具有很好的预防效果；3、由于噻苯隆与超敏蛋白的协同作用，促进了草莓的营养传导、抗病抗逆，对草莓的单果重有显著的提升作用；4、本发明能显著提高草莓含糖量，从而提高果品品种；4、噻苯隆与超敏蛋白在配比1：(0.05～90)之间时，协同增效作用效果明显，当噻苯隆与超敏蛋白的比值在小于1:90或大于1：0.05时，两者表现为相加作用。
(二)田间药效验证试验
1、噻苯隆与超敏蛋白混配对樱桃作物调节生长及抗病增产的田间综合试验
施药时期：樱桃初花期。
施药次数：1次。利用本发明的制剂实施例进行喷施，三个重复，结果取平均值。
表2噻苯隆与超敏蛋白混配对樱桃调节生长的田间综合实验
Figure PCTCN2016094034-appb-000002
Figure PCTCN2016094034-appb-000003
从表2实验数据显示，本发明的产品在樱桃初花期施用，可以有效调节樱桃的健康生长。在噻苯隆与超敏蛋白在配比1：(0.05～90)之间时，主要表现为：1、可显著提高樱桃座果率、提高产量；2、可调节樱桃植株自身免疫抵抗力，太高樱桃抗病抗逆能力，降低樱桃褐腐病发病指数；3、本发明可增加樱桃单个重量；4、本发明的组合物可提高樱桃的含糖量，降低樱桃的酸度，糖酸比显著提高，可促进樱桃口感的提高；5、实施例10、实施例12、实施例16，即噻苯隆：超敏蛋白配比小于1:90或或大于1：0.05时，，相对于单剂对照和其它实施例，协同作用效果不明显，表现为相加作用。

Claims (10)

  1. 一种含有噻苯隆和超敏蛋白的植物生长调节剂组合物，其特征在于该组合物包含A和B两种活性组分，其中活性组分A为噻苯隆，活性组分B为超敏蛋白，活性组分A和活性组分B的重量比为1：0.05～90。
  2. 根据权利要求1所述的组合物，其特征在于活性组分A和活性组分B的重量比为1：0.1～85。
  3. 根据权利要求2所述的组合物，其特征在于活性组分A和活性组分B的重量比为1：1～80。
  4. 根据权利要求3所述的组合物，其特征在于活性组分A和活性组分B的重量比为1：10～80。
  5. 根据权利要求1所述的组合物，其特征在于该组合物由活性成分和助剂制成农药上允许的剂型。
  6. 根据权利要求5所述的组合物，其特征在于所述剂型为水剂、悬浮剂、可溶液剂或微囊剂型。
  7. 根据权利要求5所述的组合物，其特征在于所述助剂选自载体、溶剂、分散剂、润湿剂、胶粘剂、增稠剂、粘合剂、表面活性剂或肥料中的一种或几种。
  8. 根据权利要求5所述的组合物，其特征在于该组合物中活性成分的质量含量为1～80％。
  9. 权利要求1～8中任意一项所述的含有噻苯隆和超敏蛋白的植物生长调节剂组合物在农业领域促进或调节植物生长的用途。
  10. 根据权利要求9所述的用途，其特征在于所述植物选自草莓或樱桃。
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