WO2017163378A1 - Récipient de culture - Google Patents

Récipient de culture Download PDF

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Publication number
WO2017163378A1
WO2017163378A1 PCT/JP2016/059439 JP2016059439W WO2017163378A1 WO 2017163378 A1 WO2017163378 A1 WO 2017163378A1 JP 2016059439 W JP2016059439 W JP 2016059439W WO 2017163378 A1 WO2017163378 A1 WO 2017163378A1
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WO
WIPO (PCT)
Prior art keywords
culture
peripheral surface
inner peripheral
approximately
side wall
Prior art date
Application number
PCT/JP2016/059439
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English (en)
Japanese (ja)
Inventor
宏昭 紀伊
魚住　孝之
泰次郎 清田
林　大輔
春男 大久保
Original Assignee
株式会社ニコン
住友ベークライト株式会社
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by 株式会社ニコン, 住友ベークライト株式会社 filed Critical 株式会社ニコン
Priority to PCT/JP2016/059439 priority Critical patent/WO2017163378A1/fr
Publication of WO2017163378A1 publication Critical patent/WO2017163378A1/fr

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/04—Flat or tray type, drawers

Definitions

  • the present invention relates to a culture vessel. More specifically, the present invention relates to a culture vessel having a meniscus effect.
  • This type of device enables culturing of cells in a plurality of samples in a culture vessel, and automatic observation / photographing within the culture environment of the cells being cultured. There are advantages such as less damage caused by environmental exposure during microscopic observation, and automatic observation, recording and management of cells in culture, reducing the burden on the cultivator.
  • Patent Document 1 discloses a layer A in which a solution that is in a gel state by containing a biological substance on the bottom of a culture vessel and being heated is frozen in an ungelled state. And a layer B frozen with an aqueous solution that does not become a gel state when heated, is superimposed on the layer A, and a cell culture device is disclosed.
  • Patent Document 2 discloses a technique for flattening at least a part of a meniscus of a culture solution by floating a transparent flat plate on the culture solution.
  • JP 2001-340070 A Japanese Patent Laid-Open No. 5-181068
  • Patent Document 1 requires a device for freezing and humidifying the solution, resulting in an increase in cost.
  • Patent Document 2 it is necessary to prepare a flat plate separately, which increases costs, and there is a possibility that bubbles are formed between the flat plate and the solution or cause contamination. is there.
  • the present invention has been made in consideration of the above points, and an object of the present invention is to provide a culture vessel that enables high-precision observation without incurring an increase in cost.
  • a bottom wall portion having a culture surface, a side wall portion having an inner peripheral surface disposed along a peripheral edge of the bottom wall portion, an outer peripheral end portion of the culture surface, and the inner portion
  • a predetermined amount of liquid is used that is provided at a predetermined distance from the peripheral surface and is surrounded by a peripheral surface having a connecting portion with the inner peripheral surface, the culture surface, the side wall, and the peripheral surface.
  • a culture container is provided in which the contact position is higher than the position of the connecting portion of the inner peripheral surface with the peripheral surface.
  • FIG. 1 is a diagram showing an embodiment of the present invention, and is a schematic external perspective view of a culture vessel 100.
  • FIG. FIG. 2 is a cross-sectional view taken along line AA in FIG.
  • FIG. 2 is a cross-sectional view taken along line AA in FIG. It is a figure which shows a mode that the culture surface 51 of the barrier surface 53 vicinity in the culture container 100 was observed. It is a figure which shows a mode that the culture surface of the side wall part vicinity was observed on the same conditions using the conventional 100mmDish.
  • FIGS. 1 to 5 show one aspect of the present invention and does not limit the present invention, and can be arbitrarily changed within the scope of the technical idea of the present invention. Moreover, in the following drawings, in order to make each configuration easy to understand, the actual structure is different from the scale and number of each structure.
  • FIG. 1 is a schematic external perspective view of the culture vessel 100.
  • 2 is a cross-sectional view taken along line AA in FIG.
  • the culture vessel 100 is surrounded by the bottom wall portion 10, the side wall portion 30 disposed along the periphery of the bottom wall portion 10, and the bottom wall portion 10 and the side wall portion 30.
  • a culture chamber 50 a culture chamber 50.
  • the peripheral surface 55 is provided at a predetermined distance between the outer peripheral end of the culture surface 53 and the inner peripheral surface 31.
  • the peripheral surface 55 is connected to the inner peripheral surface 31 via the connection portion 56.
  • the outer peripheral edge is a boundary portion of the culture surface 53 region.
  • the bottom wall portion 10 has a plate shape arranged along a horizontal plane and is formed in a rectangular shape in plan view.
  • the side wall portion 30 is arranged in a substantially rectangular frame shape (the rectangular four corner portions may be curved) on the periphery of the bottom wall portion 10b. Accordingly, the culture vessel 100 has a rectangular parallelepiped culture chamber 50 that opens upward.
  • the side wall portion 30 is disposed on the outer peripheral side, as shown in FIG. And a step 43 formed between the first outer peripheral surface 41 and the second outer peripheral surface 42 in parallel with the horizontal plane.
  • the first outer peripheral surface 41 constitutes a fitting portion into which the lid body 60 that closes the culture chamber 50 is fitted.
  • the first outer peripheral surface 41 located on the long side of the side wall portion 30 and the first outer peripheral surface 41 located on one short side in the long side direction are connected by an arcuate curved surface 44 in plan view.
  • the 1st outer peripheral surface 41 located in a long side among the side wall parts 30, and the 1st outer peripheral surface 41 located in the other short side (front side in FIG. 1) of a long side direction are circular arc by planar view. They are connected by a curved surface 45 having a shape and a curvature larger than that of the curved surface 44. That is, the first outer peripheral surface 41 is formed asymmetrically with respect to the long side direction with the short side direction as the center line. Accordingly, when the first outer peripheral surface 41 is fitted to the lid body 60, the lid body cannot be fitted unless it is in a prescribed direction, and the lid body is prevented from being assembled incorrectly.
  • the culture chamber 50 has a culture surface 51 at the bottom that is the inside of the side wall 30. At the edge of the culture surface 51, a barrier surface 53 that rises from the culture surface 51 at a height H1 is provided at a predetermined height.
  • the barrier surface 53 is formed with a gradient (inclination angle) of about 2 degrees as an example.
  • the barrier surface 53 is provided at a predetermined distance W from the inner peripheral surface 31 of the side wall portion 30.
  • the predetermined distance W in the present embodiment is defined by the distance between the lower end of the inner peripheral surface 31 and the upper end of the barrier surface 53 in the side wall portion 30.
  • a peripheral surface 55 is provided at the upper end of the barrier surface 53.
  • the peripheral surface 55 extends outward from the upper end of the barrier surface 53 and is connected to the inner peripheral surface 31 of the side wall portion 30.
  • the peripheral surface 55 is inclined at an inclination angle ⁇ gradually downward as it goes inward from the inner peripheral surface 31.
  • a height H ⁇ b> 2 from the culture surface 51 at a position where the peripheral surface 55 is connected to the inner peripheral surface 31 of the side wall portion 30 is set according to the amount of the medium put into the culture chamber 50. More specifically, the height H ⁇ b> 2 is set such that the liquid level of the medium placed in the culture chamber 50 is above the connection portion 56 between the peripheral surface 55 and the inner peripheral surface 31. In other words, the volume up to the connection portion 56 in the culture chamber 50 is set to be less than the amount of the medium used for culture in the culture chamber 50.
  • the barrier surface 53 and the peripheral surface 55 are formed over the entire circumference inside the side wall 30 as shown in FIG.
  • the peripheral surface 55 arranged inside the long side wall portion 30 and the peripheral surface 55 arranged inside the short side wall portion 30 are connected by a curved surface 57 at each intersection.
  • the curved surface 57 is inclined at the same angle as the inclination angle ⁇ of the peripheral surface 55 and is formed around an axis orthogonal to the culture surface 51. Since the barrier surface 53 and the peripheral surface 55 are formed on the inner side of the side wall portion 30 over the entire circumference, the culture surface 51 is surrounded by the barrier surface 53.
  • FIG. 3 is a cross-sectional view taken along line AA in FIG. 1 in which a culture solution (medium) B is placed in the culture chamber 50.
  • a culture solution (medium) B is placed in the culture chamber 50.
  • FIG. 3 As shown in FIG. 3, a curved meniscus M is formed on the culture surface S of the culture solution B placed in the culture chamber 50 at the contact portion with the inner peripheral surface 31 of the side wall portion 30 due to the surface tension of the liquid. Arise.
  • observation with transmitted illumination (bright field, phase difference, differential interference, etc.) is essential, but to observe the observation object. May be refracted by the meniscus M, and the light may not reach the observation target.
  • the boundary of the culture surface 51 by the barrier surface 53 is provided with a predetermined distance W from the inner peripheral surface 31.
  • the predetermined distance W is set to be larger than the distance DM from the inner peripheral surface 31 to the end of the meniscus M. Therefore, the observation target cultured on the culture surface 51 can be illuminated with observation illumination light in a state where the influence of the meniscus M is suppressed, and high-precision observation is possible.
  • the barrier surface 53 having a height H1 rising from the culture surface 51 is provided at the periphery of the culture surface 51, the cultured cells from the culture surface 51 to the barrier surface 53 side are provided. Can be prevented.
  • the peripheral surface 55 provided between the upper end of the barrier surface 53 and the inner peripheral surface 31 of the side wall 30 is gradually inclined downward at an inclination angle ⁇ as it goes inward. Therefore, it is possible to introduce cells or the like staying outside the culture surface 51 into the culture surface 51. Further, the peripheral surface 55 provided inside the long side of the side wall portion 30 and the peripheral surface 55 provided inside the short side of the side wall portion 30 are inclined at the same angle as the inclination angle ⁇ of the peripheral surface 55. Since they are connected by the curved surface 57, it is possible to introduce cells or the like staying outside the culture surface 51 into the culture surface 51 even inside the portion where the long side and the short side of the side wall portion 30 intersect.
  • the curved surface 57 it is possible to prevent cells from collecting in the corner portion at the bottom of the container. In addition, operations such as sweeping out cells with a culture instrument such as a scraper (such as a brush) are facilitated.
  • a culture instrument such as a scraper (such as a brush)
  • the size of the culture vessel 100 for example, a standardized outer shape having an outer length of 127.76 mm, an outer width of 85.48 mm, and a height of 14.35 mm can be adopted.
  • This size is one of the standards developed by SBS (the Society Society for Biomolecular Screening) and approved by ANSI (American National Standards Institute) (so-called 100 mmDish).
  • the thickness of the first outer peripheral surface 41 is about 2 to 3 mm.
  • the culture vessel 100 can be made of a transparent material.
  • the material having transparency include inorganic substances typified by glass and quartz, and organic substances typified by synthetic resins.
  • Synthetic resins include polystyrene (PS), polypropylene (PP), polymethylpentene (PMP), polycarbonate (PC), polymethyl methacrylate (PMMA), polymethylacrylmethylimide (PMMI), and cycloolefin copolymer (COC). And the like.
  • combined from two or more of the monomer units of these polymers is also mentioned.
  • the culture vessel 100 may be a structure formed by integral molding, or may be a structure formed by combining a member constituting the culture surface 51 and a member constituting the peripheral wall portion 30 and the barrier surface 53, for example.
  • each member may be made of different materials.
  • the culture surface 51, the barrier surface 53, the peripheral surface 55 and the inner peripheral surface 31 of the culture vessel 100 may be subjected to a surface treatment from the viewpoint of physical, chemical and / or biochemistry.
  • a surface treatment can be appropriately selected by those skilled in the art.
  • at least one of the barrier surface 53, the peripheral surface 55, and the inner peripheral surface 31 may be subjected to a surface treatment for inhibiting cell culture.
  • Area of the culture surface 51 in the culture container 100 substantially 55cm 2 or more, approximately 58cm 2 or less.
  • a culture area equivalent to the SBS standard 100 mmDish can be secured.
  • Many protocols such as the number of seeded cells, the amount of medium used, the amount of serum, the time to change the medium, the amount of various coatings, the time to confluence, the number of cells at confluence, etc. are built in accordance with the containers specified in the SBS standard. Yes. Therefore, when the culture vessel 100 of the present embodiment is used, it becomes possible to work while referring to the protocol constructed in the SBS standard vessel, and cell culture according to the purpose can be performed easily and without error. Is possible.
  • the predetermined distance W between the inner peripheral surface 31 and the barrier surface 53 in the side wall portion 30, that is, the predetermined distance W between the inner peripheral surface 31 and the culture surface 51 is approximately 10 mm or more and approximately 14 mm or less (when there is no barrier surface 53).
  • approximately 13 mm or less is preferable. That is, the height of the liquid surface when the same amount of liquid is accommodated varies depending on whether the barrier surface 53 is present or not. Therefore, in consideration of changes in the liquid level, when the peripheral surface 55 is inclined, the height at which the liquid surface contacts the inner peripheral surface 31 can be adjusted by changing the length of the peripheral surface 55. It becomes possible.
  • the predetermined distance W is less than about 10 mm, the distance from the inner peripheral surface 31 to the end of the meniscus M is shorter than the distance DM from the culture surface 51, and part of the illumination light that illuminates the cells on the culture surface 51 Since it passes through the meniscus M, the observation accuracy may be adversely affected.
  • the predetermined distance W exceeds approximately 13 mm, the area of the culture surface 51 becomes small, and there is a possibility that the area of approximately 55 cm 2 or more cannot be secured.
  • the predetermined distance W is about 10 mm or more and about 14 mm or less, so that the culture surface 51 can be observed with high accuracy without a part of the illumination light passing through the meniscus M, and SBS. A culture area equivalent to the standard 100 mm dish can be secured.
  • the peripheral surface 55 in the culture vessel 100 is gradually inclined downward as it goes inward from the inner peripheral surface 31, and the inclination angle ⁇ satisfies about 0 ⁇ ⁇ about 5.5.
  • the inclination angle ⁇ is 0 ° or less, that is, parallel to the horizontal surface or inclined upward in the direction from the inner peripheral surface 31 toward the inner side, the seedling is seeded and positioned outside the culture surface 51.
  • a cell that stays on the peripheral surface 55 or moves in the direction toward the inner peripheral surface 31 due to its own weight at the time of sowing may cause an undesirable situation.
  • the inclination angle ⁇ is larger than approximately 0 degrees, the cells that have been seeded and are located outside the culture surface 51 do not stay on the peripheral surface 55, and do not stay on the peripheral surface 55. It moves along the inclination of 55 and can be introduced into the culture surface 51.
  • the barrier surface 53 needs to have a height that can prevent the cells grown on the culture surface 51 from growing on the peripheral surface 55.
  • the amount of medium generally used in the SBS standard 100 mm dish is about 10 ml, but when about 10 ml of the culture medium B is put in the culture chamber 50, the liquid level of the culture liquid level S is higher than the height H2.
  • the culture liquid surface S is formed at a position in contact with the peripheral surface 55.
  • the meniscus M is formed on the inner side than the case where the culture liquid surface S is in contact with the inner peripheral surface 31, and the inner end of the meniscus M is positioned above the culture surface 51 to illuminate the cells on the culture surface 51. There is a possibility that part of the illumination light to be refracted by the meniscus M.
  • the height H1 of the barrier surface 53 is approximately 0.5 mm
  • the inclination angle ⁇ of the peripheral surface 55 is defined as approximately 0 ⁇ ⁇ approximately 5.5.
  • the seeded cells can move along the peripheral surface 55 inclined toward the culture surface 51 and be introduced into the culture surface 51, and the liquid level of the culture liquid surface S becomes higher than the height H2 so that the culture liquid surface S is changed. Since it contacts with the inner peripheral surface 31, the meniscus M can be formed at a position outside the culture surface 51, and adverse effects on the observation accuracy of cells and the like on the culture surface 51 can be suppressed.
  • the present invention may have either a container having a barrier surface 53 whose culture surface is recessed with respect to the peripheral surface 55 and a configuration without the barrier surface 53.
  • the configuration without the barrier surface 53 is a configuration in which the peripheral surface 53 is formed from the outer peripheral end of the culture surface.
  • the position of the liquid surface of the liquid accommodated in the culture chamber is important.
  • the height of the barrier surface 53 is also an important factor. Further, it is possible to adjust by the combination of the inclination angle of the peripheral surface 55 and the length of the peripheral surface 55.
  • the final product is a cell in regenerative medicine, and the cell can be a product sold as a medicine.
  • how to evaluate the quality of the cells as a product is a big issue. Unlike drugs (low molecular weight compounds), cells are alive and have different states, so sampling and testing cannot guarantee the quality of the entire product. Further, in the process of culturing, unexpected impurities (for example, those differentiated into cells different from the target cells) may easily appear. It is extremely difficult to control them to produce only a single cell. Therefore, it is very important in this field to provide a means for observing all the cells in the container in terms of improving the evaluation performance.
  • the culture vessel 100 of the present embodiment since cells and the like located outside the culture surface 51 can be introduced into the culture surface 51 via the peripheral surface 55 during seeding, the influence of the meniscus M is suppressed. In this state, it is possible to observe all the cells in the culture vessel 100 with high accuracy. Therefore, when the culture container 100 of the present embodiment is used, it is possible to accurately perform image analysis such as cell counting and morphological analysis based on the result observed with high accuracy.
  • the culture vessel 100 was manufactured by injection molding using polystyrene.
  • the outer shape of the culture vessel 100 was 127.76 mm in the long side direction and 85.48 mm in the short side direction.
  • the culture surface 51 in the culture vessel 100 has a shape in which the length in the long side direction is 100 mm, the length in the short side direction is 58 mm, and the corner portion is connected in an arc shape with a radius of 17.5 mm.
  • the area of the culture surface 51 was about 5537 mm 2 (55.37 cm 2 ).
  • the width of the peripheral surface 55 in the culture vessel 100 was 10.5 mm (entire circumference) in both the long side direction and the short side direction from the lower end of the inner peripheral surface 31 to the upper end of the barrier surface 53. Further, the inclination angle ⁇ of the peripheral surface 55 was set to 5 degrees. The height H2 at this time was approximately 1.42 mm.
  • the culture solution B 10 ml of the culture solution B was put into the culture chamber 50 in the culture vessel 100.
  • a basal medium DMEM Dulbecco-Folkto modified Eagle's minimum essential medium
  • the culture solution B uses “mTeSR1 (registered trademark)” manufactured by Veritas Co., Ltd. and “Stemfit (registered trademark)” manufactured by Ajinomoto Co., Inc. Can do.
  • the culture liquid level S was formed at a position about 0.1 mm higher than the height H2.
  • the inner end portion of the meniscus M was formed at a position outside the culture surface 51.
  • FIG. 4 is a view showing a state in which the culture surface 51 in the vicinity of the barrier surface 53 in the culture vessel 100 is observed.
  • FIG. 5 is a figure which shows a mode that the culture surface of the side wall part vicinity was observed on the same conditions using the conventional 100 mmDish.
  • FIG. 4 when observed using the culture vessel 100, it was confirmed that a uniform image could be observed without being affected by the meniscus M.
  • FIG. 5 when observed using a 100 mm dish, uneven illumination occurs between the container center and the side wall due to the influence of the meniscus M, and a wide range of cell observation is performed with high accuracy. It was difficult.
  • the volume up to the connection in the culture chamber was less than about 10 ml.
  • the volume up to the connection in the culture chamber The volume can be about 11 ml or less.
  • the basic condition is that approximately 10 ml of the culture solution is accommodated in the culture chamber, but it is also possible to accommodate 10 ml or more of liquid.
  • the influence of the meniscus can be reduced.
  • the volume to the connection part of the culture chamber may be less than about 20 ml. That is, in the case of a dedicated container in which the amount of liquid to be stored is limited, it may be assumed that the volume up to the connection portion is set to be less than the amount of liquid to be used.
  • the barrier surface 53 and the peripheral surface 55 illustrated the structure formed over the perimeter along the side wall part 30, it is not limited to this structure, All the circumferences The structure provided only in one part may be sufficient.
  • the circumferential lengths of the barrier surface 53 and the peripheral surface 55 are preferably at least twice the predetermined distance W. By making the length in the circumferential direction of the barrier surface 53 and the peripheral surface 55 more than twice the predetermined distance W, the influence of the meniscus M formed in the region where the barrier surface 53 and the peripheral surface 55 are not provided is eliminated.
  • the culture surface 51 can be observed in the state.
  • the structure which the culture container 100 has a rectangular external shape was illustrated, it is good also as a culture container circular in planar view other than a rectangular shape.
  • the size of the culture container is preferably a size based on the above-mentioned 100 mmDish and the area of the culture surface is about 55 cm 2 or more and about 58 cm 2 or less.
  • the equation of approximately 0 ⁇ ⁇ approximately 5.5 is defined as the inclination angle ⁇ at which the culture solution surface S does not contact the peripheral surface 55.
  • the inclination angle may exceed the angle defined by the above formula. That is, the inclination angle ⁇ may be increased within a range where the culture liquid surface S does not contact the peripheral surface 55.

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  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Le but de la présente invention concerne un récipient de culture qui permet une observation hautement précise sans augmenter les coûts. Ce récipient de culture comporte : une partie (10) de paroi inférieure présentant une surface de culture (51) ; une partie (30) de paroi latérale disposée le long du bord périphérique de la partie de paroi inférieure et présentant une surface périphérique interne (31) ; une surface (55) de bord périphérique disposée de façon à écarter le bord périphérique externe de la surface de culture et la surface périphérique interne l'une de l'autre d'une distance prédéterminée et présentant une partie contiguë (56) qui est contiguë à la surface périphérique interne ; et une chambre de culture pour la culture à l'aide d'une quantité prédéterminée de liquide, la chambre de culture étant entourée par la surface de culture, la partie de paroi latérale et la surface de bord périphérique. Le volume de la chambre de culture par rapport à la partie contiguë est inférieur à celui de la quantité prédéterminée. La quantité prédéterminée est une quantité de liquide qui est contenue dans la chambre de culture de telle sorte que la surface de liquide est en contact avec la surface périphérique interne en une position au-dessus de la partie contiguë où la surface périphérique interne est contiguë à la surface de bord périphérique.
PCT/JP2016/059439 2016-03-24 2016-03-24 Récipient de culture WO2017163378A1 (fr)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019039035A1 (fr) * 2017-08-25 2019-02-28 富士フイルム株式会社 Dispositif, procédé et programme d'apprentissage d'un dispositif de détermination et dispositif de détermination
CN110068558A (zh) * 2018-01-24 2019-07-30 思纳福(北京)医疗科技有限公司 微液滴容器

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59203489A (ja) * 1983-05-02 1984-11-17 ベクトン・デイツキンソン・アンド・カンパニ− ペトリ皿
JPS6251977A (ja) * 1985-09-02 1987-03-06 Terumo Corp 組織培養プレ−ト
JPH01243983A (ja) * 1988-02-16 1989-09-28 Api Syst 微生物分析用容器またはその組立体
WO1991006624A1 (fr) * 1989-10-26 1991-05-16 Costar Corporation Cuvette de fecondation in vitro
WO2014107811A1 (fr) * 2013-01-10 2014-07-17 Stemcell Technologies Inc. Élément de réduction de ménisque
WO2016047751A1 (fr) * 2014-09-25 2016-03-31 住友ベークライト株式会社 Récipient de culture

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS59203489A (ja) * 1983-05-02 1984-11-17 ベクトン・デイツキンソン・アンド・カンパニ− ペトリ皿
JPS6251977A (ja) * 1985-09-02 1987-03-06 Terumo Corp 組織培養プレ−ト
JPH01243983A (ja) * 1988-02-16 1989-09-28 Api Syst 微生物分析用容器またはその組立体
WO1991006624A1 (fr) * 1989-10-26 1991-05-16 Costar Corporation Cuvette de fecondation in vitro
WO2014107811A1 (fr) * 2013-01-10 2014-07-17 Stemcell Technologies Inc. Élément de réduction de ménisque
WO2016047751A1 (fr) * 2014-09-25 2016-03-31 住友ベークライト株式会社 Récipient de culture

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019039035A1 (fr) * 2017-08-25 2019-02-28 富士フイルム株式会社 Dispositif, procédé et programme d'apprentissage d'un dispositif de détermination et dispositif de détermination
US11328522B2 (en) 2017-08-25 2022-05-10 Fujifilm Corporation Learning device, method, and program for discriminator, and discriminator
CN110068558A (zh) * 2018-01-24 2019-07-30 思纳福(北京)医疗科技有限公司 微液滴容器

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