WO2017109244A1 - Sensor electroquímico y procedimiento de recubrimiento, procedimiento de fabricación y usos correspondientes - Google Patents
Sensor electroquímico y procedimiento de recubrimiento, procedimiento de fabricación y usos correspondientes Download PDFInfo
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- WO2017109244A1 WO2017109244A1 PCT/ES2016/070832 ES2016070832W WO2017109244A1 WO 2017109244 A1 WO2017109244 A1 WO 2017109244A1 ES 2016070832 W ES2016070832 W ES 2016070832W WO 2017109244 A1 WO2017109244 A1 WO 2017109244A1
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- Prior art keywords
- plasma
- detection
- organic polymer
- carbon
- sensor
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- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/28—Electrolytic cell components
- G01N27/30—Electrodes, e.g. test electrodes; Half-cells
- G01N27/327—Biochemical electrodes, e.g. electrical or mechanical details for in vitro measurements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/62—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving urea
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/66—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood sugars, e.g. galactose
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/82—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving vitamins or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
- G01N33/9406—Neurotransmitters
- G01N33/9413—Dopamine
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- C—CHEMISTRY; METALLURGY
- C01—INORGANIC CHEMISTRY
- C01B—NON-METALLIC ELEMENTS; COMPOUNDS THEREOF; METALLOIDS OR COMPOUNDS THEREOF NOT COVERED BY SUBCLASS C01C
- C01B32/00—Carbon; Compounds thereof
- C01B32/15—Nano-sized carbon materials
- C01B32/158—Carbon nanotubes
Definitions
- the invention relates to an electrochemical sensor for the detection of various organic substances, such as dopamine, glucose, uric acid and / or ascorbic acid, in various body fluids, such as in blood and / or urine.
- various organic substances such as dopamine, glucose, uric acid and / or ascorbic acid
- the invention also relates to a method of coating an electrochemical sensor according to the invention, a method of manufacturing an electrochemical sensor according to the invention and various uses thereof.
- Dopamine a member of the catecholamine family, acts as an important neurotransmitter in the central nervous system of mammals by modulating vital functions, such as voluntary movement. It is related to cognitive and motor functions.
- the neurons (dopaminergic) of DA release in the central nervous system are dysfunctional or dying, causing a lack of dopamine in the target territories, which results in impaired motor functions.
- the object of the invention is to overcome these drawbacks. This purpose is achieved by a method of coating an electrochemical sensor characterized in that it comprises the steps of:
- the plasma is an atmospheric plasma, a vacuum plasma or a crown plasma of energy comprised between 0.1 mJ / cm 2 and 100 J / cm 2 in an atmosphere with oxygen or nitrogen or other inert gas.
- the organic polymer is a non-electrochemically active polymer, and is preferably a polymer of the group consisting of polyethylene, poly (tetramethylene) succinate), polypropylene, polyvinylpyrrolidone, ethylene polyoxide, poly (4-vinylphenol), polycaprolactone, polyamide PA 66, polystyrene, polyacrylic acid and cellulose.
- the organic polymer can advantageously be an electrochemically active polymer (ie, a polymer with conjugated bonds or a conductive polymer), and is preferably a polymer of the group consisting of poly (3,4-ethylenedioxythiophene) and poly (A / - cyanoethylpyrrole).
- electrochemically active polymer ie, a polymer with conjugated bonds or a conductive polymer
- the plasma application time is greater than 1 s (and advantageously it is greater than 15 s) and / or it is less than 120 s.
- the carbon-rich substrate is a material of the group consisting of graphite, vitreous carbon, nanostructured carbons (preferably graphene or carbon nanotubes) and fulerenes.
- a subject of the invention is also a method of manufacturing an electrochemical sensor comprising a carbon-rich substrate, with a carbon content greater than or equal to 50% by weight with respect to the total weight of the substrate, characterized in that it includes a treatment step plasma surface of said substrate.
- a subject of the invention is also a method of manufacturing an electrochemical sensor, comprising a carbon-rich substrate, with a carbon content greater than or equal to 50% by weight with respect to the total weight of the substrate, characterized in that it comprises a step of coating according to the invention.
- Another object of the invention is an electrochemical sensor characterized in that it comprises a carbon-rich substrate, with a carbon content greater than or equal to 50% by weight with respect to the total weight of the substrate, and a modified organic polymeric coating, where the polymeric coating Modified is obtainable by a method according to the invention.
- the invention also aims at various uses:
- the sensor is for the detection of dopamine, glucose, uric acid and / or ascorbic acid.
- a sensor according to the invention for the detection of dopamine, glucose, uric acid and / or ascorbic acid.
- Fig. 1 Control voltamograms of 100 ⁇ DA, 100 ⁇ UA and 100 ⁇ AA in a 0.1 M phosphate buffer solution (PBS) recorded using untreated substrates (electrodes): vitreous carbon (GCE) bare GCE, and GCE coated with poly (3,4-ethylenedioxythiophene) (PEDOT) and poly (A / -cyanoethylpyrrole) (PNCPy).
- PBS phosphate buffer solution
- Fig. 2 Control voltamograms of 100 ⁇ DA, 100 ⁇ UA and 100 ⁇ AA in a 0.1 M phosphate buffer solution (PBS) recorded using plasma-air treated substrates (electrodes): bare GCE, and coated GCE with poly (3,4-ethylenedioxythiophene) (PEDOT) and poly (A / -cyanoethylpyrrol) (PNCPy).
- PBS phosphate buffer solution
- Fig. 4 determination of the limit of detection of DA of the GCE coated with PEDOT and PNCPy with a plasma-air treatment.
- Fig. 5 variation of the intensity of anodic peak (/ p ) in the ECGs coated with PEDOT with cold plasma treatment with respect to the plasma application time (t cp ).
- Fig. 6 control voltamogram of 1 mM DA in 0.1 M PBS in the GCE coated with low density polyethylene (LDPE), without and with plasma-air treatment. The first and third cycle for the electrode treated with plasma-air is shown.
- Fig. 7 Control voltamograms of 100, 10 and 1 ⁇ DA in GCE coated with LPDE treated with plasma-air. Right: Complete voltamograms; Left: extension of the area associated with the oxidation of DA. In all cases: scanning speed: 100 mV / s; final and initial potentials: -0.40; Reverse potential: +0.80 V.
- Fig. 8 micrograph obtained by scanning electron microscopy (SEM) of GCE coated with PEDOT not treated with plasma.
- Fig. 9 SEM micrograph of GCE coated with plasma treated PEDOT.
- Fig. 10 cyclic voltammetry of dopamine 10 ⁇ in urine chemical simulation using a GPE coated with plasma-air treated LDPE.
- Fig. 1 extension of the cyclic voltammeters in the oxidation zone of 10 ⁇ dopamine in urine chemical simulation using a GPE coated with plasma-air treated LDPE.
- Fig. 13 intensity of the dopamine oxidation peak against oxidation cycles and reduction in urine chemical simulation using a GPE coated with plasma-air treated LDPE.
- Fig. 14 expansion of cyclic voltammeters in the oxidation zone of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GPE coated with plasma-air treated LDPE.
- PBS phosphate buffered saline, pH 7.2
- Fig. 15 intensity of the oxidation peak of the DA against oxidation and reduction cycles in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with plasma-air treated LDPE.
- Fig. 16 absolute and peak intensity of the DA using GCE coated with conventional polymer and treated with plasma-air.
- Fig. 18 DA oxidation potential using conventional polymer coated GCE and plasma-air treated.
- Fig. 19 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with isotactic polypropylene treated with and without plasma-air.
- the results will be purchased with GCE treated with and without plasma-air.
- Fig. 20 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with polyvinylpyrrolidone (approximate average molecular weight: 40,000) treated with and without plasma-air.
- PBS phosphate buffered saline, pH 7.2
- polyvinylpyrrolidone approximately average molecular weight: 40,000
- Fig. 21 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with poly (ethylene oxide) (approximate average molecular weight: 600,000) treated with y without plasma-air. The results will be purchased with GCE treated with and without plasma-air.
- Fig. 22 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with poly (4-vinylphenol) (approximate average molecular weight: 25,000) treated with y without plasma-air. The results will be purchased with GCE treated with and without plasma-air.
- Fig. 23 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with polycaprolactone (approximate average molecular weight: 7,000) treated with and without plasma-air.
- PBS phosphate buffered saline, pH 7.2
- polycaprolactone approximately average molecular weight: 7,000
- Fig. 24 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with nylon 66 (polyamide PA 66) treated with and without plasma-air.
- the results will be purchased with GCE treated with and without plasma-air.
- Fig. 25 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with polystyrene (from the manufacturer Polymer Additives) treated with and without plasma-air.
- the results will be purchased with GCE treated with and without plasma-air.
- Fig. 26 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with polyacrylic acid (25% by weight in water, approximate average molecular weight: 240,000) treated with and without plasma-air.
- PBS phosphate buffered saline
- polyacrylic acid 25% by weight in water, approximate average molecular weight: 240,000
- Fig. 27 cyclic voltammetry of oxidation of DA 10 ⁇ in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with poly (butylene succinate) (marketed under the trade name Bionolle®) treated with and without plasma-air.
- PBS phosphate buffered saline, pH 7.2
- GCE coated with poly (butylene succinate) marketed under the trade name Bionolle®
- Fig. 28 cyclic voltammetry of oxidation of 10 ⁇ DA in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GPE coated with cold plasma treated LDPE in an oxidizing and reducing atmosphere.
- Fig. 29 Current-time density graph for chronoamperometric detection of 1 mM glucose using a plasma-air-treated PEDOT coated GCE on which the glucose oxidase enzyme has been immobilized. The injection of glucose into the detection cell starts in 300 s and is performed every 100 s.
- Fig. 30 Current-time density graph for chronoamperometric detection of 1 mM glucose, 1 mM UA, 1 mM AA and 1 mM DA using a plasma-air-treated PEDOT-coated GCE on which the glucose oxidase enzyme has been immobilized .
- the injection of glucose and the different interferents into the detection cell starts in 500 s and takes place every 100 s.
- Fig. 31 determination of the glucose detection limit of the ECG coated with PEDOT and a plasma-air treatment.
- the glucose oxidase enzyme was immobilized on the electrode surface.
- One of the surprising results of the present invention is the application of a cold plasma (plasma in which ions and electrons are not in thermal equilibrium) as a very simple and effective technique for the preparation of electrochemical sensors of DA (dopamine).
- a cold plasma plasma in which ions and electrons are not in thermal equilibrium
- DA dopamine
- PNMP behavior improves when the film is coated with Gold nanoparticles (AuNPs), which demonstrates the electrocatalytic activity promoted by the latter.
- AuNPs Gold nanoparticles
- Both PEDOT and PNCPy films generated by anodic polymerization on a CGE electrode were modified by applying the cold plasma surface treatment (corona plasma in an ambient atmosphere at approximately 0.5 J / cm "2 for 2 minutes ).
- DA, UA and AA were carried out by cyclic voltammetry (CV) using a glass cell containing 10 mL of PBS (saline phosphate buffer solution) 0.1 M at room temperature .
- Figs. 1 to 5 show the voltammetric response of the CPGs coated with PNCPy and PEDOT not treated and treated with plasma. Voltamograms recorded using bare ECGs have been included for comparison. Although the plasma treatment causes a significant reduction of the anodic peak intensity at 0.70V for all systems, it should be noted that this effect is relatively reduced for the anodic intensities associated with the oxidation of the three analytes.
- both electrodes with PEDOT and with PNCP and plasma treated are capable of selectively detecting oxidation of DA, UA, and AA, while untreated PNCPy is not able to selectively discriminate between them.
- untreated PNCPy is not able to selectively discriminate between them.
- the bare ECG it is not able to selectively detect the presence of AA in the mixture, regardless of plasma treatment.
- Figs. 1 and 2 show control voltamograms of 100 ⁇ DA, 100 ⁇ UA and 100 ⁇ AA in 0.1 M PBS at bare GCE, and of the GCE coated with PEDOT and PNMPy. Arrows indicate oxidation processes. Scanning speed: 100 mV / s. Initial and final potentials: -0.40 V; reverse potential: +0.80 V.
- Fig. 4 " 1 The determination of the DA detection limit (in the absence of UA and AA) of the GCE coated with PEDOT and PNCPy with a cold plasma treatment by CV using a scan speed of 50 mV-s is shown in Fig. 4 " 1.
- the results were derived from the standard addition of 10 ⁇ of DA in 10 mL of 0.1 M PBS (ie, a linear range of 0.5 to 100 ⁇ DA).
- Anodic peak intensity (/ p ) increases with the concentration of DA for the two electrodes
- the detection limit which was determined using a calibration curve for the concentration of DA between 0.5 and 5 ⁇ (box) was between 140 and 750 nM for PEDOT and PNCPy, respectively These values are markedly lower than those obtained for untreated samples, and show an improvement not only in resolution (especially for PNCPy).
- PEDOT and PNCPy films were prepared by chronoamperometry (AC) under a constant potential of 1.40 V using a two-compartment cell and three electrodes in a nitrogen atmosphere (99.995% purity) at 25 e C.
- a vitreous carbon electrode (GCE) naked with a diameter of 2 mm was used as working electrode, whereas a sheet of steel AISI 316 with an area of 1 cm 2 was used as the counterelectrode.
- the surface of the vitreous carbon electrode was polished with alumina powder and cleaned by ultrasonication before polymer deposition.
- the reference electrode was an Ag
- AgCI electrode containing a saturated aqueous solution of KCI ⁇ 0.222 V vs.
- PEDOT and PNCPy films were obtained using 10 mM of a solution of monomer in acetonitrile with 0.1 M of LiCI0 4 and a polymerization period between 6 and 10 s, respectively.
- the ECGs coated with PEDOT and PNCPy were prepared with a corona discharge in an ambient atmosphere using a BD-20AC from the Electro-Technic Products company.
- the BD-20AC operates at a very high frequency in the MHz range, generating an electric field created around the electrode that is used for the treatment of the polymer surface.
- the unit consists of a power control unit and a separate high voltage handle. What sets it apart from other models is that it generates an adjustable high voltage output between 10,000 and 45,000 volts at a high frequency 4.5 MHz.
- the treatment of the polymers was carried out using a flat tip needle electrode (in English, spring tip wire electrode) and a voltage of 45,000 volts at a high frequency of 4.5 MHz in all cases. After plasma treatment, the coated GCE electrodes were used for DA detection experiments over a period of 24 hours.
- CV cyclic voltammetry
- low density polyethylene The low density polyethylene (LDPE) was deposited on the GCE by dissolving (34.4 mg LDPE dissolved in 10 ml of dichlorobenzene at 95 C and stirring for 4 hours).
- LDPE-coated ECG without cold plasma treatment the cyclic voltamogram recorded in a 0.1 M PBS solution with 1 mM DA does not provide for any oxidation peak (Fig. 6), indicating that, as expected , LDPE cannot detect said neurotransmitter.
- LDPE coated electrodes treated with a simple plasma-air for 1 minute are capable of detecting DA concentrations similar to those estimated for the synapse during several cycles
- FIGs. 8 and 9 SEM micrographs of GCE coated with untreated and plasma-treated PEDOT are shown, respectively.
- the relatively compact morphology of the untreated samples (Fig. 8) containing C, S, O and Cl (chlorine is due to the perchlorate dopant) is transformed into a very porous network of active species composed solely of C and O (Fig. 10). Therefore, the electrochemical activity of GCE coated with plasma-treated polymers should probably be essentially attributed to the incorporation of active species on the surface, which are possibly responsible for the detection of oxidized and reduced analytes. Similar characteristics have been observed in the GPE coated with LDPE.
- the pH of the simile is 6.2, and the chemical composition is indicated below:
- Fig. 1 An extension of the cyclic voltammeters in the oxidation zone of dopamine 10 ⁇ in urine chemical simulation using a GPE coated with plasma-air treated LDPE is shown in Fig. 1.
- Fig. 10 shows the cyclic voltammeters in the full scan.
- the oxidation potential of the Dopamine is between 0.230-0.237V, while the oxidation peak of urea and other components is 0.418-0.425V.
- Fig. 12 shows the intensity of the oxidation peak of urea and other compounds against oxidation and reduction cycles in chemical urine simulation using a GPE coated with plasma-air treated LDPE.
- the oxidation potential of urea and other compounds is between 0.418 and 0.425 V.
- the total cycles applied to the system are 10.
- the loss of intensity after 10 oxidation / reduction cycles is approximately 18%.
- Fig. 13 shows the intensity of the dopamine oxidation peak against oxidation and reduction cycles in urine chemical simulation using a GPE coated with plasma-air treated LDPE.
- the oxidation potential of dopamine is between 0.230 and 0.237V.
- the total cycles applied to the system are 10. In this case there is no loss of intensity but an increase of 25% after applying 10 oxidation / reduction cycles.
- FIG. 14 shows an extension of cyclic voltammeters in the oxidation zone of 10 ⁇ dopamine in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GCE coated with plasma-air treated LDPE.
- the inserted box shows the cyclic voltammetries in the full scan.
- the oxidation potential of dopamine is between 0.171 and 0.174V.
- Fig. 15 shows the intensity of the dopamine oxidation peak against oxidation and reduction cycles in 0.1 M PBS (phosphate buffered saline, pH 7.2) using a GPE coated with plasma-air treated LDPE.
- the oxidation potential of dopamine is between 0.171 and 0.174V.
- the total cycles applied to the system are 10.
- the loss of intensity after 10 oxidation / reduction cycles is approximately 17%. 3 - Alternative substrates with polyethylene
- ITO substrates indium and tin oxide, in English indium tin oxide
- AISI 316 stainless steel have been tested, in both cases coated with low density polyethylene. In both cases, the substrates are negatively affected with plasma application and favorable results are not obtained. 4 - Alternative polymers
- Figs. 16 to 27 show the results obtained. As can be seen, the application of plasma-air in other conventional polymers produces effects similar to those obtained with LDPE.
- the monitoring of glucose levels in the human body is essential for the diagnosis and treatment of diabetes, which has become a public health problem worldwide.
- the monitoring of glucose metabolism through the detection of changes in the concentration of this analyte can improve the treatment of brain diseases, such as tumors and brain injuries.
- Glucose detection is also very important in the food processing, fermentation and bio-fuel cells industry.
- Another surprising result of the present invention is the preparation of electrochemical glucose sensors by applying the cold plasma surface treatment (corona plasma in ambient atmosphere at approximately 0.5 J / cm "2 for 2 minutes) at polymer films deposited on a CGE.
- PNMPy improves when the film is coated with gold nanoparticles (AuNPs), which demonstrates the electrocatalytic activity promoted by the latter.
- AuNPs gold nanoparticles
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RU2018126488A RU2018126488A (ru) | 2015-12-22 | 2016-11-22 | Электрохимический датчик и способ нанесения покрытия, способ изготовления и соответствующие применения |
AU2016378671A AU2016378671A1 (en) | 2015-12-22 | 2016-11-22 | Electro-chemical sensor and coating method, production method and corresponding uses |
MX2018007699A MX2018007699A (es) | 2015-12-22 | 2016-11-22 | Sensor electroquimico y procedimiento de recubrimiento, procedimiento de fabricacion y usos correspondientes. |
CA3009433A CA3009433A1 (en) | 2015-12-22 | 2016-11-22 | Electrochemical sensor and coating method, production method and corresponding uses |
KR1020187021121A KR20180098331A (ko) | 2015-12-22 | 2016-11-22 | 전기화학적 센서, 코팅 방법, 제조 방법 및 용도 |
JP2018552916A JP2019501397A (ja) | 2015-12-22 | 2016-11-22 | 電気化学センサ並びにコーティング方法、生産方法、及び対応する使用 |
BR112018012810-4A BR112018012810A2 (pt) | 2015-12-22 | 2016-11-22 | sensor eletroquímico e método de revestimento, método de produção e usos correspondentes |
CN201680082176.3A CN108700541A (zh) | 2015-12-22 | 2016-11-22 | 电化学传感器和涂覆方法、制造方法以及相应的用途 |
US16/065,080 US11067528B2 (en) | 2015-12-22 | 2016-11-22 | Electro-chemical sensor and coating method, production method and corresponding uses |
EP16877827.2A EP3396367A4 (en) | 2015-12-22 | 2016-11-22 | ELECTROCHEMICAL SENSOR AND COATING METHOD, MANUFACTURING METHOD AND CORRESPONDING USES |
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CN110192868B (zh) * | 2019-05-24 | 2021-01-08 | 厦门大学 | 基于石墨烯复合材料的柔性钙钾离子检测传感器及其制备方法 |
CN111781258A (zh) * | 2020-02-27 | 2020-10-16 | 中国地质大学(北京) | 一种可快速检测水环境中抗生素的传感器及检测方法 |
CN114216943B (zh) * | 2021-11-26 | 2023-11-03 | 青岛科技大学 | 一种抗污染电化学免疫传感器及其制备方法与应用 |
CN115290719A (zh) * | 2022-08-26 | 2022-11-04 | 江苏鱼跃凯立特生物科技有限公司 | 一种用于尿酸检测的电化学试纸及其制备方法与应用 |
Family Cites Families (11)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
FR2590219B1 (fr) | 1985-11-20 | 1991-02-01 | Bendix France | Dispositif de freinage electrique pour vehicule |
US6103033A (en) | 1998-03-04 | 2000-08-15 | Therasense, Inc. | Process for producing an electrochemical biosensor |
US6338790B1 (en) | 1998-10-08 | 2002-01-15 | Therasense, Inc. | Small volume in vitro analyte sensor with diffusible or non-leachable redox mediator |
KR100340174B1 (ko) * | 1999-04-06 | 2002-06-12 | 이동준 | 전기화학적 바이오센서 테스트 스트립, 그 제조방법 및 전기화학적 바이오센서 |
JP2001159618A (ja) * | 1999-12-03 | 2001-06-12 | Matsushita Electric Ind Co Ltd | バイオセンサ |
US6863800B2 (en) | 2002-02-01 | 2005-03-08 | Abbott Laboratories | Electrochemical biosensor strip for analysis of liquid samples |
US7276283B2 (en) * | 2004-03-24 | 2007-10-02 | Wisconsin Alumni Research Foundation | Plasma-enhanced functionalization of carbon-containing substrates |
US20080164142A1 (en) | 2006-10-27 | 2008-07-10 | Manuel Alvarez-Icaza | Surface treatment of carbon composite material to improve electrochemical properties |
KR20140066377A (ko) * | 2012-11-23 | 2014-06-02 | 삼성전자주식회사 | 바이오 센서 |
CN103487484B (zh) | 2013-10-10 | 2015-05-27 | 佳木斯大学 | 一种超敏感石墨烯电极的制备方法 |
ES2663899B2 (es) | 2016-10-17 | 2018-09-11 | Universidade Da Coruña | Sistema para asistir a caminar |
-
0
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Non-Patent Citations (6)
Title |
---|
E. LUAIS ET AL.: "Preparation and modification of carbon nanotubes electrodes by cold plasmas processes toward the preparation of amperometric biosensors", ELECTROCHIMICA ACTA, vol. 55, no. 27, 30 November 2010 (2010-11-30), pages 7916 - 7922, XP027428402 * |
G. FABREGAT ET AL.: "A rational design for the selective detection of dopamine using conducting polymers", PHYSICAL CHEMISTRY CHEMICAL PHYSICS, vol. 16, no. 17, 7 May 2014 (2014-05-07), pages 7850 - 7861, XP055394010 * |
H. MORTENSEN ET AL.: "Modification of glassy carbon surfaces by atmospheric pressure cold plasma torch", JAPANESE JOURNAL OF APPLIED PHYSICS, vol. 45, no. 10B, 24 October 2006 (2006-10-24), pages 8506 - 8511, XP055394021 * |
J. ZHAO ET AL.: "Carbon nanotube nanoweb-bioelectrode for highly selective dopamine sensing", APPLIED MATERIALS & INTERFACES, vol. 4, no. 1, 25 January 2012 (2012-01-25), pages 44 - 48, XP055393993 * |
Q. LIU ET AL.: "Electrochemical detection of dopamine in the presence of ascorbic acid using PVP/graphene modified electrodes", TALANTA, vol. 97, 15 August 2012 (2012-08-15), pages 557 - 562, XP028450153 * |
Y. WU ET AL.: "A dopamine sensor based on a methoxypolyethylene glycol polymer covalently modified glassy carbon electrode", ANALYST, vol. 138, no. 4, 2013, pages 1204 - 1211, XP055394028 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107941875A (zh) * | 2017-11-25 | 2018-04-20 | 于世金 | 一种尿液中的尿酸的电化学的检测方法及检测电极材料 |
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ES2560577A1 (es) | 2016-02-19 |
MX2018007699A (es) | 2018-11-09 |
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EP3396367A4 (en) | 2020-01-15 |
EP3396367A1 (en) | 2018-10-31 |
CA3009433A1 (en) | 2017-06-29 |
CN108700541A (zh) | 2018-10-23 |
AU2016378671A1 (en) | 2018-08-09 |
ES2560577B2 (es) | 2017-01-17 |
KR20180098331A (ko) | 2018-09-03 |
US20190004001A1 (en) | 2019-01-03 |
JP2019501397A (ja) | 2019-01-17 |
RU2018126488A (ru) | 2020-01-23 |
US11067528B2 (en) | 2021-07-20 |
BR112018012810A2 (pt) | 2018-12-04 |
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