WO2017067168A1 - 一种防治锈病的木瓜蛋白酶络合铜剂 - Google Patents

一种防治锈病的木瓜蛋白酶络合铜剂 Download PDF

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WO2017067168A1
WO2017067168A1 PCT/CN2016/083491 CN2016083491W WO2017067168A1 WO 2017067168 A1 WO2017067168 A1 WO 2017067168A1 CN 2016083491 W CN2016083491 W CN 2016083491W WO 2017067168 A1 WO2017067168 A1 WO 2017067168A1
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papain
copper
rust
photocatalyst
complexing
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PCT/CN2016/083491
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王猛
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苏州科大微龙农业科技有限公司
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins

Definitions

  • the invention relates to a papain complexing copper agent for controlling rust, which is suitable for controlling crop rust caused by P. stipitis and Puccinia sphaeroides.
  • the function involved in the present invention belongs to the technical field of plant disease control.
  • the rust fungus causes the rust of legumes, which is the pathogen causing the rust of the grasses, which endangers the leaves, stems and fruits of the plants. It causes defoliation, focal length, and poor growth. In severe cases, the spores are densely packed together, and the crops quickly die due to the evaporation of water in the body. The distribution of rust is wide and harmful. When a large area is popular, it causes short-term harvest and even production. The situation has caused great economic losses.
  • the current effective prevention and control method is to use chemical pesticides for prevention and control.
  • the drugs include triadimefon, sulfur suspension agent, chlorothalonil, daisen zinc and stone sulphur mixture.
  • Triadimefon (Purple Ning), although the effect is obvious, but its drawbacks are also increasingly prominent, such as environmental pollution, human and animal poisoning, Sanzhi (carcinogenic, teratogenic, mutagenic) and other issues, modern people put forward more on the quality and safety of food High requirements, the above-mentioned parties face the problems caused by chemical pesticides, people's understanding of pesticides has undergone profound changes, and it is gradually proposed to be harmful to pests and target organisms, and the products that are easily decomposed and decomposed are harmless to the environment. Biologically reasonable pesticides or environmentally harmonious pesticides are good substitutes for traditional chemical pesticides. How to use biological sources and plant sources to match targets The nutritional needs of sexual crops, extraction and synthesis have developed a new type of environmentally harmonious pesticides, and the prevention and control of tomato early blight is an important issue for plant protection technicians.
  • the object of the present invention is to extract copper ions from copper hydroxide by alcohol to form a copper ion containing copper lysozyme and amino acid copper, which is achieved by the following method;
  • a component of papain complex copper agent for controlling rust is divided into:
  • the present invention prioritizes the weight ratio of the best combination components:
  • the photocatalyst of the present invention is an 80 nm anatase shell titanium spheroid
  • the preparation method of the papain complexing copper agent for controlling rust of the invention comprises: placing the green papaya in the sunlight for 3 days, further pulverizing the green papaya into a fine powder by using a pulverizer, and adding 58% into the glass bottle. Brewing wine, placed under low light for 7 days, further filter the residue, add copper hydroxide and stir evenly, further add photocatalyst, and place it in the sun irradiation or 1500 nm illumination with 1500 lumens for 3 days. Papain complexing copper agent for controlling rust;
  • the present invention extracts papain having an activity of 1000-1300 u/g from green papaya through brewing wine, and then photocatalyzes hydrogen to produce copper having an antibacterial property by complexing copper with glutamic acid;
  • the material taken by the present invention is a plant source, and the crop is not absorbed after application;
  • the antibacterial principle of the present invention is to coagulate the protein in the pathogen, hinder the entry and exit of the pathogen nutrients and to achieve the function of sterilization, and does not cause the resistance of the pathogen;
  • the preparation method of the invention is simple, low in cost, simple in use method, does not cause problems of poisoning of humans and animals, phytotoxicity of plants, and is a rust prevention and control agent which is in harmony with the environment.
  • Figure 1 shows a production process of papain complexing copper agent for controlling rust
  • Case 1 The corn was grown in the experimental field. When the corn was grown to 7 leaf age, the corn rust fungus was sprayed on the soil and the crop. After 7 days, the corn plants were examined and the yellow rust spots on the leaves were found. The disease index was 32%, in the experimental group sprayed rust prevention papain complex copper 1000 times solution, the experimental group and the control group were examined, the experimental group's disease index was found to be 8%, the control group's disease index rose to 48%, continue to spray 1000 After 7 days, the disease index of the experimental group was 2%, and the plants in the control group died dry and the disease index was 78%.
  • Case 2 A farm in Zhangjiagang, Jiangsu province, planted 10 mu of long beans in a simple greenhouse, planted in June 2015, and found yellow spots on the leaves in July. After microscopic examination, it was determined to be rust Caused by bean rust, the disease index was 17%, spraying 1000 times of rust-resistant papain complex copper, and the planting area was checked after 7 days. The disease did not appear on the new leaves. The leaves were thick and green, and were checked before pulling in September. No rust disease was found, and the growers indicated that the difference between the control and the chemical pesticides was that the leaves of the plants grew normally, the leaves were shiny, and the sweetness of the beans was strong.
  • the papain complex copper of the present invention is used to effectively inactivate the growth of the pathogen, and the chemistry is solved.
  • the use of pesticides causes unhealthy growth of crops, thus affecting the quality of finished products.
  • the use of antibiotics extracted from biological sources and plant sources in a comprehensive example not only has the effect of directly inhibiting pathogenic bacteria, but also triggers crop self-generating protective substances.
  • the invention is a green non-toxic rust prevention and treatment solution worth promoting.

Abstract

本发明公开了一种防治锈病的木瓜蛋白酶络合铜剂，属于植物病害生物防治的技术领域；其特征是由组合成分重量百分比：青木瓜25％、氢氧化铜2％、光催化剂2％、余量为58％酿造酒加至100％；制成的含有活性1000-1300u/g的木瓜蛋白酶，再经由光催化氢解出具有谷氨酸络合铜制成具有抗菌性的氨基酸铜；本发明的优点是采用植物源的原料，是环境和谐的农药，能解决目前常规使用的三唑酮化学农药产生的农药残留问题，并且能促进增产和提升产成品的质量。

Description

一种防治锈病的木瓜蛋白酶络合铜剂 技术领域
本发明涉及一种防治锈病的木瓜蛋白酶络合铜剂，针对齐单胞锈菌、柄锈菌引起的作物锈病进行防治，本发明涉及的功能属于植物病害防治的技术领域。
背景技术
单胞锈菌引起豆科植物的锈病，柄锈菌是引起禾科植物的锈病的病原体，危害植物的叶、茎、果实。造成落叶、焦梢、生长不良，严重时孢子堆密集成片，作物因体内水分大量蒸发而迅速枯死，锈病分布范围广而且危害性大，当大面积的流行时引起短收，甚至绝产的情况发生，造成极大的经济损失，目前有效的防治方法是采用化学农药进行防治，药品有三唑酮、硫悬浮剂、百菌清、代森锌、石硫合剂，在目前使用量最大的为三唑酮(粉锈宁)，虽然效果明显，但它的弊端也日渐突出，如环境污染、人畜中毒、三致(致癌、致畸形、致突变)等问题，现代人们对食品的质量、安全提出更高的要求，上述各方面对化学农药造成的问题，人们对农药的认识发生深刻的变化，逐渐地提出对有害生物、对标靶性生物安全，而且容易分解分解后的产物对环境无害等优点的生物合理农药或环境和谐农药是传统化学农药的很好替代品，如何使用生物源、植物源配合标靶性作物的营养需求，提取合成而开发出新型的环境和谐农药，针对番茄早疫病进行防治，是目前植物保护技术人员的一个重要课题。
发明内容
本发明的目的是由酒精提取木瓜粗蛋白酶后，络合氢氧化铜的铜离子，形成含有溶菌酶、氨基酸铜的木瓜蛋白酶络合铜，是用以下的方法实现；
一种防治锈病的木瓜蛋白酶络合铜剂组成分重量百分比为：
Figure PCTCN2016083491-appb-000001
本发明优先出最佳组合成分的重量百为分比：
Figure PCTCN2016083491-appb-000002
Figure PCTCN2016083491-appb-000003
本发明所述的光催化剂为80纳米锐钛型壳聚球钛；
本发明的一种防治锈病的木瓜蛋白酶络合铜剂的制备方法：将青木瓜放在太阳光下3天，进一步的将青木瓜用粉碎机粉碎成细末，放入玻璃瓶中加入58％酿造酒，放置弱光下7d，进一步的过滤残渣后，加入氢氧化铜搅拌均匀，进一步地加入光催化剂后，放置在太阳照射或具有650nm照度1500流明的光照环境下进行络合3d后即得防治锈病的木瓜蛋白酶络合铜剂；
本发明中一种防治锈病的木瓜蛋白酶络合铜剂的优点：
(1)本发明由青木瓜经由酿造酒制取出活性1000-1300u/g的木瓜蛋白酶，再经过光催化氢解出具有谷氨酸络合铜制成具有抗菌性的氨基酸铜；
(2)本发明的采取的材料为植物源，施用后作物吸收不残留；
(3)本发明的抗菌原理是凝结病原体内的蛋白，阻碍病原体营养进出与呼吸从而达到灭菌的功能，不会造成病原体的抗药性；
(4)本发明制备方法简单，成本低廉，使用方法简单，不会造成人畜中毒、植物药害的问题，是与环境和谐的锈病防治剂。
附图说明
图1一种防治锈病的木瓜蛋白酶络合铜剂的生产工艺
具体实施方式与案例
下面配合实际的使用案例说明本发明的特性
1、案例一：实验田种植玉米，在玉米生长到7叶龄，喷施玉米柄锈菌在土壤与作物上，7天后检查玉米的植株，发现叶片上长出黄色锈斑的病症，病情指数为32％，在实验组喷施防治锈病木瓜蛋白酶络合铜1000倍液，检查实验组与对照组，发现实验组的病情指数为8％，对照组的病情指数上升到48％，继续喷施1000倍液，7天后检查实验组的病情指数为2％，对照组的植株出现干枯死亡，病情指数78％；
2、案例二：江苏张家港某农场，应用简易大棚种植长豆10亩地，于2015年6月份播种，在7月份时发现叶片上长出黄色斑点，经过镜检后确定是由单胞锈菌引起的菜豆锈病，病情指数为17％，喷施防治锈病木瓜蛋白酶络合铜1000倍液，7天后检查种植区域，在新叶上未出现病情，叶片浓绿厚，于9月份拉秧前检查未发现锈病的病症出现，种植户表示使用本发明进行防治与化学农药的差别在于植株生长的叶片展开正常，叶片具有光泽，豆子的甜味较浓。
由以上的实验田与大田的实际使用实例，当发现由单胞锈菌、柄锈菌引起的锈病，使用本发明的木瓜蛋白酶络合铜有效的灭活其病原体的生长，更可解决因为化学农药的使用，造成的作物不健康生长，因而影响产成品质量下降的问题，综合实例的使用由生物源、植物源提取的抗菌素，不但有直接抑制病原菌的效果，更可引发作物自我产生保护素，从而抗击病原体的侵染，作物因为需要自我保护而产生出各种酶的活化，因而让果实更加的健康，产成品的质量增加，果实的营养素在健康的作物上生长，对于人体的吸收更好，本发明是值得推广的一种绿色无毒的锈病防治的解决方案。
虽然本发明已以较佳实施例揭示如上，然其并非用以限定本发明，任何本领域技术人员，在不脱离本发明的精神和范围内，当可作些许的修改和完善，因此本发明的保护范围当以权利要求书所界定的为准。

Claims (3)

  1. 一种防治锈病的木瓜蛋白酶络合铜剂，其特征是由组合成分重量百分比：青木瓜20-30％、氢氧化铜1-3％、光催化剂1-2％，余量为58％酿造酒加至100％；其中所述的光催化剂为80纳米锐钛型壳聚球钛。
  2. 如权利要求1的一种防治锈病的木瓜蛋白酶络合铜剂，其特征是最佳组合成分重量百分比为：青木瓜25％、氢氧化铜2％、光催化剂2％、余量为58％酿造酒加至100％。
  3. 一种防治锈病的木瓜蛋白酶络合铜剂其特征是采用下列的制备方法：将青木瓜放在太阳光下3天，进一步的将青木瓜用粉碎机粉碎成细末，放入玻璃瓶中加入58％酿造酒，放置弱光下7d，进一步过滤残渣后，加入氢氧化铜搅拌均匀，再加入光催化剂后，放置在太阳照射或具有650nm照度1500流明的光照环境下进行络合3d后即得一种防治锈病的木瓜蛋白酶络合铜剂。
PCT/CN2016/083491 2015-10-19 2016-05-26 一种防治锈病的木瓜蛋白酶络合铜剂 WO2017067168A1 (zh)

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CN105211102A (zh) * 2015-10-19 2016-01-06 苏州科大微龙农业科技有限公司 一种防治锈病的木瓜蛋白酶络合铜剂
CN108703025A (zh) * 2018-05-29 2018-10-26 海南农佳佳科技发展有限公司 一种提升番茄口感的生态种植方法
CN108812727A (zh) * 2018-08-31 2018-11-16 深圳市必发达科技有限公司 一种农用除锈组合物及其方法

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