WO2017045587A1 - 基因测序的流体控制装置 - Google Patents

基因测序的流体控制装置 Download PDF

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Publication number
WO2017045587A1
WO2017045587A1 PCT/CN2016/098890 CN2016098890W WO2017045587A1 WO 2017045587 A1 WO2017045587 A1 WO 2017045587A1 CN 2016098890 W CN2016098890 W CN 2016098890W WO 2017045587 A1 WO2017045587 A1 WO 2017045587A1
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WIPO (PCT)
Prior art keywords
reagent
sequencing
way valve
gene sequencing
bottle
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PCT/CN2016/098890
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English (en)
French (fr)
Inventor
葛良进
吴平
颜钦
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深圳市瀚海基因生物科技有限公司
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Publication of WO2017045587A1 publication Critical patent/WO2017045587A1/zh

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors

Definitions

  • the invention relates to the field of medical equipment, in particular to a fluid control device for gene sequencing.
  • single molecule sequencing technology has three technical routes: single molecule fluorescence sequencing technology, single molecule real-time synthesis sequencing technology and nanopore sequencing technology.
  • the single-molecule fluorescence sequencing technology has the characteristics of high accuracy and short sequencing time.
  • the fluid control device for single-molecule gene sequencing is a key module of the single-molecule tester, and the gene sequencing chip in the fluid control device for single-molecule gene sequencing is performed.
  • the core component of biochemical reaction and optical imaging, the fluid control device for gene sequencing usually has a plurality of fluid delivery devices, which have various reaction reagents, control reagent flow direction and flow rate, control reagent mixing and derivation to the gene sequencing chip. Functions such as waste liquid.
  • commercial fluid sequencing devices for gene sequencing mostly use robotic syringes to extract or inject sequencing reagents. The sequencing reagents take a long time in the delivery process, resulting in low efficiency of gene sequencing and high cost of use. .
  • the embodiments of the present invention are directed to the absence of the prior art, and the main object thereof is to provide a fluid control device for gene sequencing that improves the efficiency of gene sequencing.
  • an embodiment of the present invention provides a fluid control device for gene sequencing, wherein the fluid control device for gene sequencing includes a reagent component, a first multi-way valve, a first three-way valve, a gene sequencing chip, and a driver.
  • An assembly comprising a first reagent bottle containing a first reagent, a second reagent bottle containing a second reagent, and a third reagent bottle containing a third reagent, the first multi-pass valve connecting the first reagent a bottle, the second reagent bottle, the third reagent bottle, and the first three-way valve, the first multi-way valve for using the first reagent bottle, the second reagent bottle, or the a third reagent bottle and the The first three-way valve is connected.
  • the gene sequencing chip includes a first gene sequencing channel and a second gene sequencing channel, and the first three-way valve connects the first gene sequencing channel, the second gene sequencing channel, and the first multi-pass valve, The first three-way valve is configured to connect the first gene sequencing channel or the second gene sequencing channel to the first multi-pass valve.
  • the drive assembly includes a first syringe pump and a second syringe pump, the first syringe pump and the second syringe pump respectively tubing the first gene sequencing channel and the second gene sequencing channel.
  • the first reagent is a sequencing reagent
  • the first reagent bottle is a sequencing reagent bottle
  • the second reagent is a detectable group ablation reagent
  • the second reagent bottle is The group retreating reagent bottle
  • the third reagent is an imaging reagent
  • the third reagent bottle is an imaging reagent bottle
  • the first multi-pass valve comprises a first extraction port connected to the sequencing reagent bottle
  • the base is connected a second extraction port of the mass removal reagent bottle and a third extraction port connected to the imaging reagent bottle, and a liquid outlet, the liquid outlet and the first extraction port, or the second extraction port,
  • the first three-way valve comprises a liquid suction port, a first distribution port and a second distribution port, and the liquid suction port is connected to the first distribution port or the first a diversion port
  • the liquid suction port conduit is connected to the liquid outlet
  • the first gene sequencing channel and the second gene sequencing channel are respectively
  • the fluid sequencing device for gene sequencing further comprises a sequencing reagent configuration component, the sequencing reagent configuration component comprising a plurality of sequencing reagent raw material bottles, a second multi-way valve, and a second three-way valve And a third syringe pump, wherein the plurality of sequencing reagent raw material bottles are used to hold a plurality of sequencing reagent raw materials, the second multi-pass valve simultaneously pipes a plurality of the sequencing reagent raw material bottles, and the pipeline connects the second three a valve, the second three-way valve is further connected to the third syringe pump and the sequencing reagent bottle, and the third syringe pump is connected to the second three-way valve and the second multi-way valve
  • One of the sequencing reagent raw material bottles is connected, and the sequencing reagent bottle is connected to the third injection pump via the second three-way valve, wherein the third injection pump sequentially performs a plurality of the sequencing
  • the reagent raw material bottle is connected to extract a plurality of sequencing
  • a first mixer is connected between the second three-way valve and the sequencing reagent bottle, and the first mixer is provided with a plurality of first weir pipes, and a plurality of The first conduits are connected end to end and connected between the second three-way valve and the sequencing reagent bottle.
  • the number of the sequencing reagent bottles is plural, and the sequencing reagent configuration component further includes a third multi-pass valve, and the third multi-pass valve simultaneously connects a plurality of the sequencing a reagent bottle, and the second three-way valve, wherein the third injection pump is connected to one of the sequencing reagent bottles via the second three-way valve and the third multi-way valve.
  • the sequencing reagent configuration component further includes a rinse reagent bottle and a first waste liquid bottle, wherein the rinse reagent bottle is used to hold a rinse reagent, and the rinse reagent bottle passes through the second plurality a valve and the second three-way valve are connected to the third syringe pump, the first waste bottle is for containing waste liquid, and the first waste bottle is passed through the third multi-way valve and The second three-way valve is coupled to the third syringe pump.
  • the gene sequencing fluid control device further comprises an imaging reagent configuration component, the imaging reagent configuration component comprising a plurality of imaging reagent raw material bottles, a fourth multi-way valve, and a third three-way valve And a fourth syringe pump, wherein the plurality of imaging reagent raw material bottles are used to hold a plurality of imaging reagent raw materials, the fourth multi-pass valve simultaneously pipes a plurality of the imaging reagent raw material bottles, and the pipeline connects the third three a third valve, wherein the third three-way valve is further connected to the fourth syringe pump and the imaging reagent bottle, wherein the fourth syringe pump passes through the third three-way valve and the fourth multi-way valve One of the imaging reagent raw material bottles is connected, and the imaging reagent bottle is connected to the fourth injection pump via the third three-way valve, wherein the fourth injection pump sequentially performs a plurality of the imaging The reagent raw material bottle is connected to
  • the imaging reagent configuration assembly further includes a second mixer, the second mixer being coupled between the third three-way valve and the imaging reagent bottle, the first The second mixer includes a plurality of second weir tubes, and the plurality of second weir tubes are connected end to end and connected between the third three-way valve and the imaging reagent bottle.
  • the driving assembly further includes a fourth three-way valve, a fifth three-way valve, a second waste liquid bottle, and a third waste liquid bottle, wherein the fourth three-way valve is connected to the pipe Between the first syringe pump and the first gene sequencing channel, the second waste liquid bottle is also connected to the pipeline, and the fifth three-way valve is connected to the second syringe pump and the second Between the gene sequencing channels, the third waste bottle is also piped.
  • the first gene sequencing channel and the second gene The number of sequencing channels is multiple.
  • the fluid sequencing device for gene sequencing further includes a control component electrically connecting the first multi-way valve, the first three-way valve, and the gene sequencing chip And the drive assembly to control operation of the first multi-way valve, the first three-way valve, the genetic sequencing chip, and the drive assembly.
  • the fluid control device for gene sequencing is connected to the gene sequencing chip through the first multi-way valve and the first three-way valve through the reagent component, and utilizes the gene sequencing chip
  • the first gene sequencing channel and the second gene sequencing channel are set internally such that reagents of the reagent component can automatically flow into the first gene sequencing channel and the second gene sequencing channel for reaction and image acquisition And when the first gene sequencing channel performs a sequencing reaction, the second gene sequencing channel can perform image collection, thereby effectively reducing the gene sequencing time, that is, effectively improving the efficiency of gene sequencing, and the fluid for sequencing the gene.
  • the control device reduces the cost of gene sequencing and improves the efficiency of gene sequencing.
  • FIG. 1 is a schematic structural diagram of a fluid control device for gene sequencing according to an embodiment of the present invention.
  • FIG. 1 is a fluid control device 100 for gene sequencing provided by an embodiment of the present invention.
  • the gene sequencing fluid control device 100 includes a reagent assembly, a first multi-way valve 20, a first three-way valve 30, a gene sequencing chip 40, and a drive assembly 50.
  • the reagent assembly includes a first reagent bottle 11 containing a first reagent, a second reagent bottle 12 containing a second reagent, and a third reagent bottle 13 containing a third reagent, and the first multi-way valve 20 is connected to the first a reagent bottle 11, the second reagent bottle 12, the third reagent bottle 13 and the first three-way valve 30, the first multi-way valve 20 is for using the first reagent bottle 11, The second reagent bottle 12 or the third reagent bottle 13 is connected to the first three-way valve 30.
  • the gene sequencing chip 40 includes a first gene sequencing channel 41 and a second gene sequencing channel 42, the first three-way a valve 30 connecting the first gene sequencing channel 41, the second gene sequencing channel 42 and the first multi-way valve 20, the first three-way valve 30 for using the first gene sequencing channel 41 or
  • the second gene sequencing channel 42 is coupled to the first multi-way valve 20.
  • the drive assembly 50 includes a first syringe pump 51 and a second syringe pump 52, and the first syringe pump 51 and the second syringe pump 52 respectively connect the first gene sequencing channel 41 and the second gene Sequencing channel 42.
  • the second syringe pump 52 can stop providing a negative pressure; when the first syringe pump 51 stops providing the negative pressure, the second syringe pump 52 can provide a negative pressure, such that The sequencing efficiency of the fluid control device 100 capable of improving gene sequencing.
  • the first syringe pump 51 supplies a negative pressure to the first gene sequencing channel 41 such that the first gene sequencing channel 41 sequentially acquires the first reagent, the second reagent, and the first
  • the second syringe pump 52 stops providing a negative pressure to the second gene sequencing channel 42 to cause the second gene sequencing channel 42 to perform image acquisition.
  • the fluid control device 100 for gene sequencing of the above embodiment is connected to the gene sequencing chip 40 via the first multi-way valve 20 and the first three-way valve 30 through the reagent assembly, and utilizes the The first gene sequencing channel 41 and the second gene sequencing channel 42 are disposed in the gene sequencing chip 40 such that reagents of the reagent component can automatically flow into the first gene sequencing channel 41 and the second gene
  • the reaction and image acquisition are performed in the sequencing channel 42, and when the first gene sequencing channel 41 performs a sequencing reaction, the second gene sequencing channel 42 can perform image collection, thereby effectively reducing the gene sequencing time, that is, effectively improving
  • the efficiency of gene sequencing enables the fluid control device 100 for sequencing the gene to reduce the cost of gene sequencing and improve the efficiency of gene sequencing.
  • the first reagent is a sequencing reagent
  • the first reagent bottle 11 is a sequencing reagent bottle 11
  • the second reagent is a detectable group excision reagent
  • the second reagent bottle 12 is a base.
  • the reagent bottle 12 is removed
  • the third reagent is an imaging reagent
  • the third reagent bottle 13 is an imaging reagent bottle 13.
  • the first multi-way valve 20 includes a first extraction port 21 connected to the sequencing reagent bottle 11, a second extraction port 22 connecting the group excision reagent bottle 12, and a third connection to the imaging reagent bottle 13.
  • the port 23 is extracted and a sequencing outlet 24 is provided.
  • the liquid outlet 24 is connected to the first extraction port 21, the second extraction port 22 or the third extraction port 23.
  • the first three-way valve 30 includes a liquid suction port 31, a first split port 32, and a second split port 33, and the liquid suction port 31 opens the first split port 32 or the second split port 33.
  • the liquid suction port 31 is connected
  • the liquid outlet 24 is connected.
  • the first gene sequencing channel 41 and the second gene sequencing channel 42 are respectively connected to the first split port 32 and the second split port 33.
  • the second syringe pump 52 stops providing negative pressure to the second gene sequencing channel 42 for image acquisition by the second gene sequencing channel 42.
  • the so-called second reagent is a fluorophore excision reagent
  • the third reagent is a fluorescent imaging reagent, such that multiple acquisitions of the fluorescent image can be achieved.
  • the open conduit of the sequencing reagent bottle 11 is connected to the first extraction port 21 .
  • the sequencing reagent bottle 11 is made of a transparent glass test tube.
  • the opening of the sequencing reagent bottle 11 is disposed at the top end of the sequencing reagent bottle 11, the opening is plugged with a plug (not labeled), and the plug is inserted with an infusion tube (not In addition, the infusion tube extends into the bottom of the sequencing reagent bottle 11, so that the sequencing reagent of the sequencing reagent bottle 11 can be sufficiently extracted.
  • the sequencing reagent bottle 10 can also be a cartridge.
  • the group excision reagent bottle 12 and the imaging reagent bottle 13 have the same structure as the sequencing reagent bottle 11, and will not be described herein.
  • the first multi-way valve 20 is a rotary valve, and the first extraction port 21, the second extraction port 22, and the third extraction port 23 respectively surround the liquid outlet 24,
  • the first extraction port 21, the second extraction port 22, and the third extraction port 23 are connected to the liquid outlet 24 by a rotating duct 25 that rotates around the liquid outlet 24.
  • the rotating pipe 25 can be sequentially rotated to the positions of the first extraction port 21, the second extraction port 22, and the third extraction port 23, so that the liquid outlet 24 can sequentially turn on the sequencing reagent bottle 11,
  • the group excision reagent bottle 12 and the imaging reagent bottle 13, that is, the gene sequencing chip 40 may sequentially obtain reagents from the sequencing reagent bottle 11, the group excision reagent bottle 12, and the imaging reagent bottle 13. , in turn, gene sequencing reactions and image acquisition.
  • the order in which the liquid outlet 24 and the first extraction port 21, the second extraction port 22, and the third extraction port 23 are turned on may not be limited.
  • the liquid suction port 31 of the first three-way valve 30 when the liquid suction port 31 of the first three-way valve 30 is connected to the first split port 32, the liquid suction port 31 is disconnected from the second split port 33.
  • the liquid suction port 31 may be configured to turn on the first split port 32 or the second split port 33 according to a gene sequencing cycle, that is, when the first gene sequencing channel 41 performs a gene sequencing reaction in one cycle,
  • the first split port 32 is connected to the liquid suction port 30, so that the liquid suction port 30 supplies a reaction reagent to the first gene sequencing channel 41 via the first split port 32, and at this time
  • the second split port 33 is disconnected from the liquid suction port 31, so that the second The reagents in the gene sequencing channel 42 stop flowing, thereby enabling image acquisition and photographing.
  • the second split port 33 is connected to the liquid suction port 31, and the second gene sequencing channel 42 starts to receive the reaction through the liquid suction port 31.
  • the reagent and the gene sequencing reaction are performed, and the first gene sequencing channel 41 stops acquiring the reaction reagent and performs image collection, thereby effectively reducing the gene sequencing time and improving the gene sequencing efficiency.
  • the two ends of the first gene sequencing channel 41 are respectively connected to the first split port 32 of the first three-way valve 30 and the first syringe pump 51.
  • the two ends of the second gene sequencing channel 42 are respectively connected to the second split port 33 of the first three-way valve 30 and the second syringe pump 52.
  • the second gene sequencing channel 42 performs image acquisition and photographing; when the second gene sequencing channel 42 performs the gene sequencing reaction, the first gene is sequenced.
  • the channel 41 can perform image acquisition and photographing, so that the first gene sequencing channel 41 and the second gene sequencing channel 42 operate in coordination with each other, thereby effectively reducing gene sequencing time, thereby improving gene sequencing efficiency.
  • the first syringe pump 51 and the second syringe pump 52 respectively perform sequencing of the gene sequencing reaction in the first gene sequencing channel 41 and the second gene sequencing channel 42 by using a negative pressure. Waste liquid to ensure stable sequencing of the gene.
  • the gene sequencing fluid control device 100 further includes a sequencing reagent configuration component 60 including a plurality of sequencing reagent raw material bottles 61, a second multi-way valve 62, a second three-way valve 63, and The third syringe pump 64.
  • a plurality of the sequencing reagent raw material bottles 61 are used to hold a plurality of sequencing reagent raw materials
  • the second multi-way valve 62 simultaneously pipes a plurality of the sequencing reagent raw material bottles 61, and the pipes are connected to the second three-way valves 63.
  • the second three-way valve 63 is also piped to the third syringe pump 64 and the sequencing reagent bottle 11.
  • the third syringe pump 64 is connected to one of the sequencing reagent raw material bottles 61 via the second three-way valve 63 and the second multi-way valve 62.
  • the sequencing reagent bottle 11 is connected to the third syringe pump 64 via the second three-way valve 63.
  • the third injection pump 64 is sequentially connected to the plurality of sequencing reagent raw material bottles 61 to extract a plurality of sequencing reagent raw materials in the sequencing reagent raw material bottles 61, and is mixed and configured into the sequencing reagents.
  • the third syringe pump 64 is connected to the sequencing reagent bottle 11 for injecting the sequencing reagent into the sequencing reagent bottle 11.
  • the plurality of sequencing reagent raw material bottles 61 respectively contain different sequencing reagent raw materials, so that the sequencing reagent raw materials in the plurality of sequencing reagent raw material bottles 61 can be sequentially extracted by the third injection pump 64, thereby The mixture is configured as a sequencing reagent.
  • the number of the sequencing reagent raw material bottles 61 is ten.
  • the structure of the sequencing reagent raw material bottle 61 is set in the same manner as the structure of the sequencing reagent bottle 11.
  • a plurality of the sequencing reagent raw material bottles 61 may be placed on a test tube rack to stabilize a plurality of the sequencing reagent raw material bottles 61, and different labels may be respectively attached to the six sequencing reagent raw material bottles 61.
  • the number of sequencing reagent raw material bottles 61 may also be six or eight.
  • the second multi-way valve 62 is disposed in the same configuration as the first multi-way valve 20. Differently, the second multi-way valve 62 realizes that the third syringe pump 64 is sequentially connected to a plurality of the sequencing reagent raw material bottles 61, and the second multi-way valve 62 selects one of the sequencing units.
  • the reagent raw material bottle 61 is turned on, and the third injection pump 64 is controlled to adjust the amount of the sampling reagent raw material extracted in the sequencing reagent raw material bottle 61 by controlling the ON time. Thereby, the sequencing reagent raw materials of the plurality of the sequencing reagent raw material bottles 61 can be configured in proportion to meet the requirements of gene sequencing.
  • the second three-way valve 63 is configured in the same manner as the first three-way valve 30.
  • the second three-way valve 63 can realize that the third syringe pump 64 and the second multi-way valve 62 are turned on, so that the third syringe pump 64 can extract a plurality of the sequencing reagent raw material bottles 61.
  • the sequencing reagent raw material is configured as a sequencing reagent.
  • the second three-way valve 63 can realize that the third syringe pump 64 and the sequencing reagent bottle 11 are turned on, so that the third syringe pump 64 can inject the already configured sequencing into the sequencing reagent bottle 11. Reagents.
  • the third syringe pump 64 may provide a negative pressure to the plurality of sequencing reagent raw material bottles 61 via the second three-way valve 63 and the second multi-way valve 62 to extract a plurality of the sequencing reagent raw material bottles. Sequencing reagent in 61.
  • the third syringe pump 64 can also provide a positive pressure to the sequencing reagent bottle 11 via the second three-way valve 63 to inject a sequencing reagent into the sequencing reagent bottle 11.
  • a first mixer 65 is connected between the second three-way valve 63 and the sequencing reagent bottle 11, and the first mixer 65 is provided with a plurality of first weir pipes 651, and the plurality of A plurality of tubes 651 are connected end to end and connected between the second three-way valve 63 and the sequencing reagent bottle 11.
  • a plurality of the first weir pipes 651 are fixed on one fixed plate, the first weir pipes 651 are S-shaped, and the plurality of the weir pipes 651 may be juxtaposed in multiple rows, and each The rows are connected to each other.
  • a plurality of the first weir conduits 651 are connected between the second three-way valve 63 and the sequencing reagent bottle 11 such that the sequencing reagent injected from the third syringe pump 64 passes through a plurality of The pipeline 651 is buffered, and the flow stroke of the sequencing reagent is increased, so that the plurality of gene sequencing reagent raw materials in the sequencing reagent are thoroughly mixed to improve the reaction efficiency of the sequencing reagent.
  • multiple The weir pipe 651 may also be sequentially spiraled.
  • the sequencing reagent configuration component 60 further includes a third multi-way valve 66, which simultaneously pipes a plurality of the sequencing reagent bottles 11 And the second three-way valve 63, the third syringe pump 64 is connected to one of the sequencing reagent bottles 11 via the second three-way valve 63 and the third multi-way valve 66.
  • the sequencing reagents in the plurality of sequencing reagent bottles 11 are different, and the number of the sequencing reagent bottles 11 is four.
  • the sequencing reagent raw material reagents of the plurality of the sequencing reagent raw material bottles 61 are different according to the third injection pump 64, and can be configured as different sequencing reagents, so that the plurality of the sequencing reagent bottles 11 can be used to hold a plurality of different types. Sequencing reagents.
  • the third multi-way valve 66 has the same structure as the second multi-way valve 62. The third multi-way valve 66 can realize that the third syringe pump 64 sequentially injects different sequencing reagents into the plurality of sequencing reagent bottles 11 respectively.
  • one of the sequencing reagent bottles 11 is selected through the second three-way valve 63 and the third multi-way valve 66, and the sequencing reagent is injected. Up to the sequencing reagent bottle 11. In other embodiments, the number of sequencing reagent bottles 11 may also be five, six or seven.
  • the sequencing reagent configuration component 60 further includes a rinse reagent bottle 67 and a first waste liquid bottle 68 for containing a rinse reagent, and the rinse reagent bottle 67 passes through the second multi-way valve 62 and the second three-way valve 63 are connected to the third syringe pump 64, the first waste liquid bottle 68 is for containing waste liquid, and the first waste liquid bottle 68 is subjected to the third The valve 66 and the second three-way valve 63 are connected to the third syringe pump 64.
  • the third syringe pump 64 may extract the flushing reagent
  • the reagent 67 is flushed in the bottle 67 to flush the third syringe pump 64, that is, after the third syringe pump 64 is configured to complete a sorting reagent, the flushing reagent bottle may be extracted before the next sequencing reagent is configured.
  • the flushing reagent in 67 is flushed and then the sequencing reagent is configured again to avoid cross-infection with two different gene sequencing configurations.
  • the third injection pump 64 When the first waste liquid bottle 68 is connected to the third injection pump 64 via the third multi-way valve 66 and the second three-way valve 63, the third injection pump 64 may have been completed.
  • the cleaned waste liquid is injected into the first waste liquid bottle 68 to achieve an environmentally friendly recycling effect.
  • the gene sequencing fluid control device 100 further includes an imaging reagent configuration component 70 including a plurality of imaging reagent raw material bottles 71, a fourth multi-way valve 72, a third three-way valve 73, and The fourth syringe pump 74. a plurality of the imaging reagent raw material bottles 71 for containing a plurality of imaging reagents material.
  • the fourth multi-way valve 72 simultaneously pipes a plurality of the image forming reagent raw material bottles 71, and pipes are connected to the third three-way valve 73.
  • the third three-way valve 73 is also piped to the fourth syringe pump 74 and the imaging reagent bottle 13.
  • the fourth syringe pump 74 is connected to one of the imaging reagent material bottles 71 via the third three-way valve 73 and the fourth multi-way valve 72.
  • the imaging reagent bottle 13 is connected to the fourth syringe pump 74 via the third three-way valve 73, wherein the fourth syringe pump 74 is sequentially connected to a plurality of the imaging reagent raw material bottles 71. And extracting a plurality of imaging reagent raw materials in the imaging reagent raw material bottle 71, and mixing and configuring the imaging reagents.
  • the fourth syringe pump 74 is coupled to the imaging reagent bottle 13 for injecting the imaging reagent into the imaging reagent bottle 13.
  • the plurality of imaging reagent raw material bottles 71 respectively contain different imaging reagent raw materials, so that the imaging reagent raw materials in the plurality of imaging reagent raw material bottles 71 can be sequentially extracted by the fourth injection pump 74, thereby
  • the mixture is configured as an imaging reagent.
  • the number of the imaging reagent raw material bottles 71 is five.
  • the structure of the imaging reagent material bottle 71 is set in the same manner as the structure of the sequencing reagent bottle 11.
  • a plurality of the imaging reagent raw material bottles 71 may be placed on the test tube rack to stabilize the plurality of the imaging reagent raw material bottles 71, and different labels may be respectively attached to the five imaging reagent raw material bottles 71. In order to facilitate the next time the imaging reagent raw material is replenished, the cross infection of the imaging reagent raw material is avoided.
  • the number of imaging reagent material bottles 71 may also be six or eight.
  • the fourth multi-way valve 72 is disposed in the same configuration as the first multi-way valve 20. Differently, the fourth multi-way valve 72 realizes that the fourth syringe pump 74 is sequentially connected to a plurality of the imaging reagent raw material bottles 71, and the fourth multi-way valve 72 selects one of the images.
  • the reagent raw material bottle 71 is turned on, and the adjustment of the amount of the image forming reagent raw material in the image forming reagent raw material bottle 71 is controlled by the fourth syringe pump 74 by controlling the ON time.
  • the imaging reagent raw materials of the plurality of the imaging reagent raw material bottles 71 can be configured in proportion to meet the needs of gene sequencing.
  • the third three-way valve 73 is configured in the same manner as the first three-way valve 30.
  • the third three-way valve 73 can realize that the fourth syringe pump 74 and the fourth multi-way valve 72 are turned on, so that the fourth syringe pump 74 can extract a plurality of the imaging reagent raw material bottles 71.
  • the imaging reagent material is configured as an imaging reagent.
  • the third three-way valve 73 can realize that the fourth syringe pump 74 and the imaging reagent bottle 13 are turned on, so that the fourth syringe pump 74 can inject the already configured imaging into the imaging reagent bottle 13 Reagents.
  • the fourth syringe pump 74 may provide a negative pressure to the plurality of imaging reagent raw material bottles 71 via the third three-way valve 73 and the fourth multi-way valve 72 to extract a plurality of the imaging reagent raw material bottles. Imaging reagent material in 71.
  • the fourth syringe pump 74 can also pass through the third three-way valve 73 into the imaging reagent bottle 13 A positive pressure is provided to inject an imaging reagent into the imaging reagent vial 13.
  • the imaging reagent configuration assembly 70 further includes a second mixer 75 coupled between the third three-way valve 73 and the imaging reagent bottle 13, the second mixing The device 75 includes a plurality of second weir ducts 751 connected end to end and connected between the third three-way valve 73 and the imaging reagent bottle 13.
  • the second mixer 75 is configured in the same manner as the first mixer 65, and the imaging reagent injected from the fourth syringe pump 74 by the second mixer 75 passes through the plurality of the second weir pipes 751.
  • the buffering is performed, and the flow stroke of the imaging reagent is increased, so that the plurality of imaging reagent materials in the imaging reagent are sufficiently mixed to improve the reaction efficiency of the sequencing reagent.
  • the drive assembly 50 further includes a fourth three-way valve 53, a fifth three-way valve 54, a second waste liquid bottle 55, and a third waste liquid bottle 56.
  • the fourth three-way valve 53 is connected between the first syringe pump 51 and the first gene sequencing channel 41, and is also connected to the second waste liquid bottle 55.
  • the fifth three-way valve 54 is piped between the second syringe pump 52 and the second gene sequencing channel 42 while also pipe connecting the third waste bottle 56.
  • the first syringe pump 51 turns on the first gene sequencing channel 41 or the second waste liquid bottle 55 via the fourth three-way valve 53, so that the first syringe pump 51 extracts the first gene
  • the waste liquid may be injected into the second waste liquid bottle 55, so that the first injection pump 51 performs the next supply to the first gene sequencing channel 41.
  • the fifth three-way valve 54 is configured in the same manner as the fourth three-way valve 53.
  • the third waste liquid bottle 56 and the second waste liquid bottle 55 are similarly arranged. No longer.
  • the number of the first gene sequencing channel 41 and the second gene sequencing channel 42 are both plural. By using a plurality of the first gene sequencing channel 41 and the second gene sequencing channel 42 , a plurality of gene sequencing reactions can be simultaneously performed, thereby further improving the efficiency of gene sequencing.
  • the number of the first syringe pump 51 and the second syringe pump 52 may also be plural, and the plurality of the first syringe pumps 51 respectively provide a negative pressure to the plurality of the first gene sequencing channels 41.
  • a plurality of the second syringe pumps 52 respectively provide a negative pressure to the plurality of the second gene sequencing channels 42.
  • a plurality of the first syringe pump 51 and a plurality of the second syringe pumps 52 may be driven by means of a row brake, such that a plurality of the first syringe pumps 51 or a plurality of the second injections
  • the pump 52 can be driven simultaneously to ensure the accuracy of the sequencing efficiency of the gene.
  • the gene sequencing fluid control device 100 further includes a control component (not shown), The control component electrically connects the first multi-way valve 20, the first three-way valve 30, the gene sequencing chip 40, and the driving assembly 50 to control the first multi-way valve 20 and the first three-way valve 30
  • the gene sequencing chip 40 and the drive assembly 50 operate.
  • the control component may be a single chip microcomputer, a calculator processor, or a central control processor, etc., and the first multi-way valve 20, the first three-way valve 30, the gene sequencing chip 40, and the driver are controlled by the control component.
  • the assembly 50 is operated, and the fluid control device 100 that implements the sequencing of the genes is automatically operated to put the gene side suction efficiency.
  • control component is further electrically connected to the second multi-way valve 62, the second three-way valve 63, the third multi-way valve 66, the fourth multi-way valve 72, the third three-way valve 73, and the The three-injection pump 64 and the fourth syringe pump 74 increase the operational efficiency of the gene-sequencing fluid control device 100.
  • the fluid control device for gene sequencing is connected to the gene sequencing chip through the first multi-way valve and the first three-way valve through the reagent component, and is set by using the gene sequencing chip
  • the first gene sequencing channel and the second gene sequencing channel such that reagents of the reagent component can automatically flow into the first gene sequencing channel and the second gene sequencing channel for reaction and image acquisition, and
  • the second gene sequencing channel can perform image collection, thereby effectively reducing the gene sequencing time, that is, the fluid sequencing device that effectively improves the gene sequencing efficiency and the gene sequencing Reduce the cost of gene sequencing and improve the efficiency of gene sequencing.

Abstract

一种基因测序的流体控制装置，所述基因测序的流体控制装置包括试剂组件、第一多通阀、第一三通阀、基因测序芯片和驱动组件，所述试剂组件经所述第一多通阀和所述第一三通阀与所述基因测序芯片相连接，并利用所述基因测序芯片内设置所述第一基因测序通道和所述第二基因测序通道，从而使得所述试剂组件的试剂可以自动流入所述第一基因测序通道和所述第二基因测序通道内进行反应和图像采集，而且在所述第一基因测序通道进行测序反应时，所述第二基因测序通道可以进行图像采集，从而有效减少了基因测序时间，即有效提高了基因测序效率，使得所述基因测序的流体控制装置减少了基因测序成本，提高基因测序效率。

Description

基因测序的流体控制装置
优先权信息
本申请请求2015年9月15日向中国国家知识产权局提交的、专利申请号为201510585327.4的专利申请的优先权和权益，并且通过参照将其全文并入此处。
技术领域
本发明涉及医疗设备领域，尤其涉及一种基因测序的流体控制装置。
背景技术
目前单分子测序技术有三种技术路线：单分子荧光测序技术、单分子实时合成测序技术和纳米孔测序技术。单分子荧光测序技术，具有准确率高、测序时间短的特点，单分子基因测序的流体控制装置是单分子测试仪的关键模块，而单分子基因测序的流体控制装置中的基因测序芯片是进行生化反应和光学成像的核心部件，基因测序的流体控制装置通常设置多个流体输送器件，该流体输送器件具有向基因测序芯片输送各种反应试剂、控制试剂流向和流量、控制试剂的混合、导出废弃液等功能。目前商用的基因测序的流体控制装置多采用机械手进样针的方式对测序试剂的提取或进样，测序试剂在输送过程中耗时较长，导致基因测序的效率较低，且使用成本较高。
发明内容
有鉴于此，本发明实施方式针对现有技术存在之缺失，其主要目的是提供一种提高基因测序效率的基因测序的流体控制装置。
为实现上述目的，本发明实施方式提供一种基因测序的流体控制装置，其中，所述基因测序的流体控制装置包括试剂组件、第一多通阀、第一三通阀、基因测序芯片和驱动组件，所述试剂组件包括盛装第一试剂的第一试剂瓶、盛装第二试剂的第二试剂瓶和盛装第三试剂的第三试剂瓶，所述第一多通阀连接所述第一试剂瓶、所述第二试剂瓶、所述第三试剂瓶及所述第一三通阀，所述第一多通阀用于将所述第一试剂瓶、所述第二试剂瓶或所述第三试剂瓶与所述 第一三通阀相接通。所述基因测序芯片包括第一基因测序通道和第二基因测序通道，所述第一三通阀连接所述第一基因测序通道、所述第二基因测序通道及所述第一多通阀，所述第一三通阀用于将所述第一基因测序通道或所述第二基因测序通道与所述第一多通阀相接通。所述驱动组件包括第一注射泵和第二注射泵，所述第一注射泵和所述第二注射泵分别管道连接所述第一基因测序通道和所述第二基因测序通道。
其中，在本发明一个实施方式中，所述第一试剂为测序试剂，所述第一试剂瓶为测序试剂瓶，所述第二试剂为可检测基团切除试剂，所述第二试剂瓶为基团切除试剂瓶，所述第三试剂为成像试剂，所述第三试剂瓶为成像试剂瓶，所述第一多通阀包括连接所述测序试剂瓶的第一抽取口、连接所述基团切除试剂瓶的第二抽取口和连接于所述成像试剂瓶的第三抽取口，以及一个出液口，所述出液口与所述第一抽取口、或所述第二抽取口、或所述第三抽取口相接通，所述第一三通阀包括吸液口、第一分流口和第二分流口，所述吸液口接通所述第一分流口或所述第二分流口，所述吸液口管道连接所述出液口，所述第一基因测序通道和所述第二基因测序通道分别管道连接所述第一分流口和所述第二分流口。
其中，在本发明一个实施方式中，所述基因测序的流体控制装置还包括测序试剂配置组件，所述测序试剂配置组件包括多个测序试剂原料瓶、第二多通阀、第二三通阀和第三注射泵，多个所述测序试剂原料瓶用以盛装多种测序试剂原料，所述第二多通阀同时管道连接多个所述测序试剂原料瓶，以及管道连接所述第二三通阀，所述第二三通阀还管道连接所述第三注射泵和所述测序试剂瓶，所述第三注射泵经所述第二三通阀和所述第二多通阀与其中一个所述测序试剂原料瓶相接通，所述测序试剂瓶经所述第二三通阀与所述第三注射泵相接通，其中，所述第三注射泵依次与多个所述测序试剂原料瓶相接通，以抽取多个所述测序试剂原料瓶中的测序试剂原料，并混合配置成所述测序试剂，所述第三注射泵与所述测序试剂瓶相接通，用于将所述测序试剂注射至所述测序试剂瓶。
其中，在本发明一个实施方式中，所述第二三通阀和所述测序试剂瓶之间连接有第一混合器，所述第一混合器设置多个第一蜿蜒管道，多个所述第一蜿蜒管道首尾相连，并接通于所述第二三通阀和所述测序试剂瓶之间。
其中，在本发明一个实施方式中，所述测序试剂瓶的数目为多个，所述测序试剂配置组件还包括第三多通阀，所述第三多通阀同时管道连接多个所述测序试剂瓶，以及所述第二三通阀，所述第三注射泵经所述第二三通阀和所述第三多通阀与其中一个所述测序试剂瓶相接通。
其中，在本发明一个实施方式中，所述测序试剂配置组件还包括冲洗试剂瓶和第一废液瓶，所述冲洗试剂瓶用于盛装冲洗试剂，所述冲洗试剂瓶经所述第二多通阀和所述第二三通阀与所述第三注射泵相接通，所述第一废液瓶用于盛装废液，所述第一废液瓶经所述第三多通阀和所述第二三通阀与所述第三注射泵相接通。
其中，在本发明一个实施方式中，所述基因测序的流体控制装置还包括成像试剂配置组件，所述成像试剂配置组件包括多个成像试剂原料瓶、第四多通阀、第三三通阀和第四注射泵，多个所述成像试剂原料瓶用以盛装多种成像试剂原料，所述第四多通阀同时管道连接多个所述成像试剂原料瓶，以及管道连接所述第三三通阀，所述第三三通阀还管道连接所述第四注射泵和所述成像试剂瓶，所述第四注射泵经所述第三三通阀和所述第四多通阀与其中一个所述成像试剂原料瓶相接通，所述成像试剂瓶经所述第三三通阀与所述第四注射泵相接通，其中，所述第四注射泵依次与多个所述成像试剂原料瓶相接通，以抽取多个所述成像试剂原料瓶中的成像试剂原料，并混合配置成成像试剂，所述第四注射泵与所述成像试剂瓶相接通，用于将所述成像试剂注射至所述成像试剂瓶内。
其中，在本发明一个实施方式中，所述成像试剂配置组件还包括第二混合器，所述第二混合器连接于所述第三三通阀和所述成像试剂瓶之间，所述第二混合器包括多个第二蜿蜒管道，多个所述第二蜿蜒管道首尾相连，并接通于所述第三三通阀和所述成像试剂瓶之间。
其中，在本发明一个实施方式中，所述驱动组件还包括第四三通阀、第五三通阀、第二废液瓶和第三废液瓶，所述第四三通阀管道连接于所述第一注射泵和所述第一基因测序通道之间，同时还管道连接所述第二废液瓶，所述第五三通阀管道连接于所述第二注射泵和所述第二基因测序通道之间，同时还管道连接所述第三废液瓶。
其中，在本发明一个实施方式中，所述第一基因测序通道和所述第二基因 测序通道的数目均为多个。
其中，在本发明一个实施方式中，所述基因测序的流体控制装置还包括控制组件，所述控制组件电连接所述第一多通阀、所述第一三通阀、所述基因测序芯片和所述驱动组件，以控制所述第一多通阀、所述第一三通阀、所述基因测序芯片和所述驱动组件运行。
本发明实施方式提供的基因测序的流体控制装置，通过所述试剂组件经所述第一多通阀和所述第一三通阀与所述基因测序芯片相连接，并利用所述基因测序芯片内设置所述第一基因测序通道和所述第二基因测序通道，从而使得所述试剂组件的试剂可以自动流入所述第一基因测序通道和所述第二基因测序通道内进行反应和图像采集，而且在所述第一基因测序通道进行测序反应时，所述第二基因测序通道可以进行图像采集，从而有效减少了基因测序时间，即有效提高了基因测序效率，使得所述基因测序的流体控制装置减少了基因测序成本，提高基因测序效率。
附图说明
为更清楚地阐述本发明的构造特征和功效，下面结合附图与具体实施方式来对其进行详细说明。
图1为本发明实施方式提供的基因测序的流体控制装置的结构示意图。
具体实施方式
下面将结合本发明实施方式中的附图，对本发明实施方式中的技术方案进行清楚、完整地描述。
请一并参阅图1，本发明实施方式提供的一种基因测序的流体控制装置100。所述基因测序的流体控制装置100包括试剂组件、第一多通阀20、第一三通阀30、基因测序芯片40和驱动组件50。所述试剂组件包括盛装第一试剂的第一试剂瓶11、盛装第二试剂的第二试剂瓶12和盛装第三试剂的第三试剂瓶13，所述第一多通阀20连接所述第一试剂瓶11、所述第二试剂瓶12、所述第三试剂瓶13及所述第一三通阀30，所述第一多通阀20用于将所述第一试剂瓶11、所述第二试剂瓶12或所述第三试剂瓶13与所述第一三通阀30相接通。所述基因测序芯片40包括第一基因测序通道41和第二基因测序通道42，所述第一三通 阀30连接所述第一基因测序通道41、所述第二基因测序通道42及所述第一多通阀20，所述第一三通阀30用于将所述第一基因测序通道41或所述第二基因测序通道42与所述第一多通阀20相接通。
所述驱动组件50包括第一注射泵51和第二注射泵52，所述第一注射泵51和所述第二注射泵52分别管道连接所述第一基因测序通道41和所述第二基因测序通道42。
工作时，较佳地，第一注射泵51提供负压时，第二注射泵52可停止提供负压；第一注射泵51停止提供负压时，第二注射泵52可提供负压，这样能提高基因测序的流体控制装置100的测序效率。例如，在所述第一注射泵51向所述第一基因测序通道41提供负压，以使所述第一基因测序通道41依次获取所述第一试剂、所述第二试剂和所述第三试剂进行基因测序反应时，所述第二注射泵52停止向所述第二基因测序通道42提供负压，以使所述第二基因测序通道42进行图像采集。
因此，上述实施方式的基因测序的流体控制装置100，通过所述试剂组件经所述第一多通阀20和所述第一三通阀30与所述基因测序芯片40相连接，并利用所述基因测序芯片40内设置所述第一基因测序通道41和所述第二基因测序通道42，从而使得所述试剂组件的试剂可以自动流入所述第一基因测序通道41和所述第二基因测序通道42内进行反应和图像采集，而且在所述第一基因测序通道41进行测序反应时，所述第二基因测序通道42可以进行图像采集，从而有效减少了基因测序时间，即有效提高了基因测序效率，使得所述基因测序的流体控制装置100减少了基因测序成本，提高基因测序效率。
在一个实施方式中，所述第一试剂为测序试剂，所述第一试剂瓶11为测序试剂瓶11，所述第二试剂为可检测基团切除试剂，所述第二试剂瓶12为基团切除试剂瓶12，所述第三试剂为成像试剂，所述第三试剂瓶13为成像试剂瓶13。所述第一多通阀20包括连接所述测序试剂瓶11的第一抽取口21、连接所述基团切除试剂瓶12的第二抽取口22和连接于所述成像试剂瓶13的第三抽取口23，以及一个测序出液口24。所述出液口24与所述第一抽取口21、或所述第二抽取口22或所述第三抽取口23相接通。
所述第一三通阀30包括吸液口31、第一分流口32和第二分流口33，所述吸液口31接通所述第一分流口32或所述第二分流口33。所述吸液口31管道连 接所述出液口24。所述第一基因测序通道41和所述第二基因测序通道42分别管道连接所述第一分流口32和所述第二分流口33。
在所述第一注射泵51向所述第一基因测序通道41提供负压，以使所述第一基因测序通道41依次获取所述测序试剂、可检测基团切除试剂和成像试剂进行基因测序反应时，所述第二注射泵52停止向所述第二基因测序通道42提供负压，以使所述第二基因测序通道42进行图像采集。
在一个实施方式中，所称第二试剂为荧光基团切除试剂，所述第三试剂为荧光成像试剂，如此可实现荧光图像的多次采集。
本实施方式中，所述测序试剂瓶11的开口管道连接于所述第一抽取口21。所述测序试剂瓶11采用透明玻璃试管制成，所述测序试剂瓶11的开口设置于所述测序试剂瓶11的顶端，开口塞有堵头(未标注)，堵头插有输液管(未标注)，所述输液管均伸入到所述测序试剂瓶11的底部，从而可以充分抽取所述测序试剂瓶11的测序试剂。在其他实施方式中，所述测序试剂瓶10还可以是盒体。
所述基团切除试剂瓶12和所述成像试剂瓶13与所述测序试剂瓶11的结构相同，在此不再赘述。
本实施方式中，所述第一多通阀20为旋转阀，所述第一抽取口21、第二抽取口22和所述第三抽取口23分别围绕于所述出液口24周围，所述第一抽取口21、第二抽取口22和所述第三抽取口23通过绕所述出液口24旋转的旋转管道25接通所述出液口24。所述旋转管道25可以依次旋转至所述第一抽取口21、第二抽取口22和第三抽取口23的位置，从而实现所述出液口24可以依次接通所述测序试剂瓶11、所述基团切除试剂瓶12和所述成像试剂瓶13，即所述基因测序芯片40可以依次从所述测序试剂瓶11、所述基团切除试剂瓶12和所述成像试剂瓶13获取试剂，进而基因测序反应和图像采集。在其他实施方式中，所述出液口24与所述第一抽取口21、第二抽取口22和第三抽取口23的接通顺序也可以不限制。
本实施方式中，所述第一三通阀30的吸液口31与所述第一分流口32相接通时，所述吸液口31与所述第二分流口33断开。所述吸液口31可以是按照基因测序周期接通所述第一分流口32或所述第二分流口33，即当所述第一基因测序通道41在一个周期内进行基因测序反应时，所述第一分流口32与所述吸液口30相接通，从而所述吸液口30经所述第一分流口32向所述第一基因测序通道41提供反应试剂，而此时所述第二分流口33与所述吸液口31断开，从而使得所述第二 基因测序通道42内的反应试剂停止流动，从而实现图像采集拍照。
待所述第二基因测序通道42完成图像采集后，所述第二分流口33与所述吸液口31相接通，所述第二基因测序通道42开始经所述吸液口31获取反应试剂，并进行基因测序反应，所述第一基因测序通道41则停止获取反应试剂，并进行图像采集，从而有效减少基因测序时间，提高基因测序效率。
本实施方式中，所述第一基因测序通道41的两端分别管道连接所述第一三通阀30的第一分流口32和所述第一注射泵51。所述第二基因测序通道42的两端分别管道连接所述第一三通阀30的第二分流口33和所述第二注射泵52。所述第一基因测序通道41内进行基因测序反应时，所述第二基因测序通道42内进行图像采集拍照；所述第二基因测序通道42内进行基因测序反应时，所述第一基因测序通道41可以进行图像采集拍照，从而所述第一基因测序通道41和所述第二基因测序通道42相互协调运行，从而有效减少基因测序时间，从而提高基因测序效率。
本实施方式中，所述第一注射泵51和所述第二注射泵52分别利用负压抽取所述第一基因测序通道41和所述第二基因测序通道42内已经完成基因测序反应的测序废液，从而保证基因测序反应稳定进行。
进一步地，所述基因测序的流体控制装置100还包括测序试剂配置组件60，所述测序试剂配置组件60包括多个测序试剂原料瓶61、第二多通阀62、第二三通阀63和第三注射泵64。多个所述测序试剂原料瓶61用以盛装多种测序试剂原料，所述第二多通阀62同时管道连接多个所述测序试剂原料瓶61，以及管道连接所述第二三通阀63。所述第二三通阀63还管道连接所述第三注射泵64和所述测序试剂瓶11。所述第三注射泵64经所述第二三通阀63和所述第二多通阀62与其中一个所述测序试剂原料瓶61相接通。所述测序试剂瓶11经所述第二三通阀63与所述第三注射泵64相接通。其中，所述第三注射泵64依次与多个所述测序试剂原料瓶61相接通，以抽取多个所述测序试剂原料瓶61中的测序试剂原料，并混合配置成所述测序试剂，所述第三注射泵64与所述测序试剂瓶11相接通，用于将所述测序试剂注射至所述测序试剂瓶11。
本实施方式中，多个所述测序试剂原料瓶61分别盛装不同的测序试剂原料，从而可以利用所述第三注射泵64依次抽取多个所述测序试剂原料瓶61中的测序试剂原料，从而混合配置成测序试剂。
具体的，所述测序试剂原料瓶61的数目为十个。所述测序试剂原料瓶61的结构与所述测序试剂瓶11的结构相同设置。多个所述测序试剂原料瓶61可以是放置于试管架上，以便对多个所述测序试剂原料瓶61进行稳固，同时还可以分别对六个所述测序试剂原料瓶61贴设不同标签，以方便下次进行测序试剂原料进行补充，避免测序试剂原料的交叉感染。在其他实施方式中，所述测序试剂原料瓶61的数目还可以是六个或八个。
所述第二多通阀62与所述第一多通阀20的结构相同设置。不同的是，所述第二多通阀62实现所述第三注射泵64依次和多个所述测序试剂原料瓶61相接通，所述第二多通阀62选定其中一个所述测序试剂原料瓶61接通，并通过控制接通时长，从而控制所述第三注射泵64对该测序试剂原料瓶61内测序试剂原料抽取量的调节。从而实现多个所述测序试剂原料瓶61的测序试剂原料可以按比例进行配置，以符合基因测序需求。
所述第二三通阀63与所述第一三通阀30结构相同设置。所述第二三通阀63可以实现所述第三注射泵64和所述第二多通阀62接通，从而所述第三注射泵64可以抽取多个所述测序试剂原料瓶61内的测序试剂原料，配置成测序试剂。所述第二三通阀63可以实现所述第三注射泵64和所述测序试剂瓶11接通，从而所述第三注射泵64可以向所述测序试剂瓶11中注射已经配置好的测序试剂。
所述第三注射泵64可以经所述第二三通阀63和所述第二多通阀62向多个所述测序试剂原料瓶61提供负压，以抽取多个所述测序试剂原料瓶61中的测序试剂。所述第三注射泵64还可以经所述第二三通阀63向所述测序试剂瓶11中提供正压，以注射测序试剂至所述测序试剂瓶11内。
进一步地，所述第二三通阀63和所述测序试剂瓶11之间连接有第一混合器65，所述第一混合器65设置多个第一蜿蜒管道651，多个所述第一蜿蜒管道651首尾相连，并接通于所述第二三通阀63和所述测序试剂瓶11之间。
本实施方式中，多个所述第一蜿蜒管道651固定于一个固定板上，所述第一蜿蜒管道651呈S形，多个所述蜿蜒管道651可以是多排并列，且每排之间相互接通。利用多个所述第一蜿蜒管道651接通于所述第二三通阀63和所述测序试剂瓶11之间，使得从所述第三注射泵64注射出的测序试剂经过多个所述蜿蜒管道651进行缓冲，而且增加测序试剂的流动行程，从而使得测序试剂中的多种基因测序试剂原料进行充分混合，提升测序试剂反应效率。在其他实施方式中，多个 所述蜿蜒管道651还可以是依次盘旋。
进一步地，所述测序试剂瓶11的数目为多个，所述测序试剂配置组件60还包括第三多通阀66，所述第三多通阀66同时管道连接多个所述测序试剂瓶11，以及所述第二三通阀63，所述第三注射泵64经所述第二三通阀63和所述第三多通阀66与其中一个所述测序试剂瓶11相接通。
本实施方式中，多个所述测序试剂瓶11内的测序试剂不同，所述测序试剂瓶11的数目为四个。根据所述第三注射泵64抽取多个所述测序试剂原料瓶61的测序试剂原料试剂比例不同，可以配置成不同的测序试剂，从而可以利用多个所述测序试剂瓶11盛装多种不同的测序试剂。所述第三多通阀66结构与所述第二多通阀62结构相同设置。所述第三多通阀66可以实现所述第三注射泵64依次向多个所述测序试剂瓶11内分别注射不同的测序试剂。具体的，所述第三注射泵64每配置完成测序试剂后，经所述第二三通阀63和所述第三多通阀66选定一个所述测序试剂瓶11，并将测序试剂注射至该测序试剂瓶11内。在其他实施方式中，所述测序试剂瓶11的数目还可以是五个、六个或七个。
进一步地，所述测序试剂配置组件60还包括冲洗试剂瓶67和第一废液瓶68，所述冲洗试剂瓶67用于盛装冲洗试剂，所述冲洗试剂瓶67经所述第二多通阀62和所述第二三通阀63与所述第三注射泵64相接通，所述第一废液瓶68用于盛装废液，所述第一废液瓶68经所述第三多通阀66和所述第二三通阀63与所述第三注射泵64相接通。
所述冲洗试剂瓶67经所述第二多通阀62和所述第二三通阀63与所述第三注射泵64相接通时，所述第三注射泵64可以抽取所述冲洗试剂瓶67内冲洗试剂，以对所述第三注射泵64进行冲洗，即所述第三注射泵64在配置完成一种测序试剂后，下一次配置测序试剂之前，可以先抽取所述冲洗试剂瓶67内的冲洗试剂，进行冲洗后再次配置测序试剂，从而避免配置两种不同的基因测序出现交叉感染。所述第一废液瓶68经所述第三多通阀66和所述第二三通阀63与所述第三注射泵64相接通时，所述第三注射泵64可以将已经完成清洗的废液注射至所述第一废液瓶68中，从而达到环保回收的效果。
进一步地，所述基因测序的流体控制装置100还包括成像试剂配置组件70，所述成像试剂配置组件70包括多个成像试剂原料瓶71、第四多通阀72、第三三通阀73和第四注射泵74。多个所述成像试剂原料瓶71用以盛装多种成像试剂原 料。所述第四多通阀72同时管道连接多个所述成像试剂原料瓶71，以及管道连接所述第三三通阀73。所述第三三通阀73还管道连接所述第四注射泵74和所述成像试剂瓶13。所述第四注射泵74经所述第三三通阀73和所述第四多通阀72与其中一个所述成像试剂原料瓶71相接通。所述成像试剂瓶13经所述第三三通阀73与所述第四注射泵74相接通，其中，所述第四注射泵74依次与多个所述成像试剂原料瓶71相接通，以抽取多个所述成像试剂原料瓶71中的成像试剂原料，并混合配置成成像试剂。所述第四注射泵74与所述成像试剂瓶13相接通，用于将所述成像试剂注射至所述成像试剂瓶13内。
本实施方式中，多个所述成像试剂原料瓶71分别盛装不同的成像试剂原料，从而可以利用所述第四注射泵74依次抽取多个所述成像试剂原料瓶71中的成像试剂原料，从而混合配置成成像试剂。具体的，所述成像试剂原料瓶71的数目为五个。所述成像试剂原料瓶71的结构与所述测序试剂瓶11的结构相同设置。多个所述成像试剂原料瓶71可以是放置于试管架上，以便对多个所述成像试剂原料瓶71进行稳固，同时还可以分别对五个所述成像试剂原料瓶71贴设不同标签，以方便下次进行成像试剂原料补充，避免成像试剂原料的交叉感染。在其他实施方式中，所述成像试剂原料瓶71的数目还可以是六个或八个。
所述第四多通阀72与所述第一多通阀20的结构相同设置。不同的是，所述第四多通阀72实现所述第四注射泵74依次和多个所述成像试剂原料瓶71相接通，所述第四多通阀72选定其中一个所述成像试剂原料瓶71接通，并通过控制接通时长，从而控制所述第四注射泵74对该成像试剂原料瓶71内成像试剂原料抽取量的调节。从而实现多个所述成像试剂原料瓶71的成像试剂原料可以按比例进行配置，以符合基因测序需求。
所述第三三通阀73与所述第一三通阀30结构相同设置。所述第三三通阀73可以实现所述第四注射泵74和所述第四多通阀72接通，从而所述第四注射泵74可以抽取多个所述成像试剂原料瓶71内的成像试剂原料，配置成成像试剂。所述第三三通阀73可以实现所述第四注射泵74和所述成像试剂瓶13接通，从而所述第四注射泵74可以向所述成像试剂瓶13中注射已经配置好的成像试剂。
所述第四注射泵74可以经所述第三三通阀73和所述第四多通阀72向多个所述成像试剂原料瓶71提供负压，以抽取多个所述成像试剂原料瓶71中的成像试剂原料。所述第四注射泵74还可以经所述第三三通阀73向所述成像试剂瓶13中 提供正压，以注射成像试剂至所述成像试剂瓶13内。
进一步地，所述成像试剂配置组件70还包括第二混合器75，所述第二混合器75连接于所述第三三通阀73和所述成像试剂瓶13之间，所述第二混合器75包括多个第二蜿蜒管道751，多个所述第二蜿蜒管道751首尾相连，并接通于所述第三三通阀73和所述成像试剂瓶13之间。
所述第二混合器75与所述第一混合器65结构相同设置，所述第二混合器75从所述第四注射泵74注射出的成像试剂经过多个所述第二蜿蜒管道751进行缓冲，而且增加成像试剂的流动行程，从而使得成像试剂中的多种成像试剂原料进行充分混合，提升测序试剂反应效率。
进一步地，所述驱动组件50还包括第四三通阀53、第五三通阀54、第二废液瓶55和第三废液瓶56。所述第四三通阀53管道连接于所述第一注射泵51和所述第一基因测序通道41之间，同时还管道连接所述第二废液瓶55。所述第五三通阀54管道连接于所述第二注射泵52和所述第二基因测序通道42之间，同时还管道连接所述第三废液瓶56。
所述第一注射泵51经所述第四三通阀53接通所述第一基因测序通道41或所述第二废液瓶55，从而所述第一注射泵51抽取所述第一基因测序通道41内已经完成基因测序反应的废液后，可以向所述第二废液瓶55注射废液，从而使得所述第一注射泵51进行下一次向所述第一基因测序通道41提供负压，以进行基因测序反应。所述第五三通阀54与所述第四三通阀53结构相同设置，在此不再赘述，所述第三废液瓶56和所述第二废液瓶55结构相同设置，在此不再赘述。
进一步地，所述第一基因测序通道41和所述第二基因测序通道42的数目均为多个。利用所述第一基因测序通道41和所述第二基因测序通道42的数目均设置多个，可以使得多个基因测序反应同时进行，从而进一步提高基因测序效率。同样原理，所述第一注射泵51和所述第二注射泵52的数目也可以是多个，多个所述第一注射泵51对应向多个所述第一基因测序通道41提供负压，多个所述第二注射泵52对应向多个所述第二基因测序通道42提供负压。多个所述第一注射泵51和多个所述第二注射泵52可以是采用联排制动的方式进行驱动，从而使多个所述第一注射泵51或多个所述第二注射泵52可以同时进行驱动，从而保证所述基因测序效率的准确性。
进一步地，所述基因测序的流体控制装置100还包括控制组件(未图示)， 所述控制组件电连接所述第一多通阀20、第一三通阀30、基因测序芯片40和所述驱动组件50，以控制所述第一多通阀20、第一三通阀30、基因测序芯片40和所述驱动组件50运行。所述控制组件可以是单片机、计算器处理器、或中央控制处理器等，利用所述控制组件控制所述第一多通阀20、第一三通阀30、基因测序芯片40和所述驱动组件50运行，实现所述基因测序的流体控制装置100自动运行，提上基因侧吸效率。本实施方式中，所述控制组件还电连接所述第二多通阀62、第二三通阀63、第三多通阀66、第四多通阀72、第三三通阀73、第三注射泵64和第四注射泵74，使得所述基因测序的流体控制装置100运行效率提升。
本发明提供的基因测序的流体控制装置，通过所述试剂组件经所述第一多通阀和所述第一三通阀与所述基因测序芯片相连接，并利用所述基因测序芯片内设置所述第一基因测序通道和所述第二基因测序通道，从而使得所述试剂组件的试剂可以自动流入所述第一基因测序通道和所述第二基因测序通道内进行反应和图像采集，而且在所述第一基因测序通道进行测序反应时，所述第二基因测序通道可以进行图像采集，从而有效减少了基因测序时间，即有效提高了基因测序效率，使得所述基因测序的流体控制装置减少了基因测序成本，提高基因测序效率。
以上所述是本发明的优选实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本发明原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也视为本发明的保护范围。

Claims (11)

  1. 一种基因测序的流体控制装置，其特征在于，所述基因测序的流体控制装置包括试剂组件、第一多通阀、第一三通阀、基因测序芯片和驱动组件，所述试剂组件包括盛装第一试剂的第一试剂瓶、盛装第二试剂的第二试剂瓶和盛装第三试剂的第三试剂瓶，所述第一多通阀连接所述第一试剂瓶、所述第二试剂瓶、所述第三试剂瓶及所述第一三通阀，所述第一多通阀用于将所述第一试剂瓶、所述第二试剂瓶或所述第三试剂瓶与所述第一三通阀相接通；
    所述基因测序芯片包括第一基因测序通道和第二基因测序通道，所述第一三通阀连接所述第一基因测序通道、所述第二基因测序通道及所述第一多通阀，所述第一三通阀用于将所述第一基因测序通道或所述第二基因测序通道与所述第一多通阀相接通；
    所述驱动组件包括第一注射泵和第二注射泵，所述第一注射泵和所述第二注射泵分别管道连接所述第一基因测序通道和所述第二基因测序通道。
  2. 根据权利要求1所述的基因测序的流体控制装置，其特征在于，所述第一试剂为测序试剂，所述第一试剂瓶为测序试剂瓶，所述第二试剂为可检测基团切除试剂，所述第二试剂瓶为基团切除试剂瓶，所述第三试剂为成像试剂，所述第三试剂瓶为成像试剂瓶，所述第一多通阀包括连接所述测序试剂瓶的第一抽取口、连接所述基团切除试剂瓶的第二抽取口和连接于所述成像试剂瓶的第三抽取口，以及一个出液口，所述出液口与所述第一抽取口、或所述第二抽取口、或所述第三抽取口相接通，所述第一三通阀包括吸液口、第一分流口和第二分流口，所述吸液口接通所述第一分流口或所述第二分流口，所述吸液口管道连接所述出液口，所述第一基因测序通道和所述第二基因测序通道分别管道连接所述第一分流口和所述第二分流口。
  3. 根据权利要求2所述的基因测序的流体控制装置，其特征在于，所述基因测序的流体控制装置还包括测序试剂配置组件，所述测序试剂配置组件包括多个测序试剂原料瓶、第二多通阀、第二三通阀和第三注射泵，多个所述测序试剂原料瓶用以盛装多种测序试剂原料，所述第二多通阀同时管道连接多个所述测序试剂原料瓶，以及管道连接所述第二三通阀，所述第二三通阀还管道连接所述第三注射泵和所述测序试剂瓶，所述第三注射泵经所述第二三通阀和所 述第二多通阀与其中一个所述测序试剂原料瓶相接通，所述测序试剂瓶经所述第二三通阀与所述第三注射泵相接通，其中，所述第三注射泵依次与多个所述测序试剂原料瓶相接通，以抽取多个所述测序试剂原料瓶中的测序试剂原料，并混合配置成所述测序试剂，所述第三注射泵与所述测序试剂瓶相接通，用于将所述测序试剂注射至所述测序试剂瓶。
  4. 根据权利要求3所述的基因测序的流体控制装置，其特征在于，所述第二三通阀和所述测序试剂瓶之间连接有第一混合器，所述第一混合器设置多个第一蜿蜒管道，多个所述第一蜿蜒管道首尾相连，并接通于所述第二三通阀和所述测序试剂瓶之间。
  5. 根据权利要求3所述的基因测序的流体控制装置，其特征在于，所述测序试剂瓶的数目为多个，所述测序试剂配置组件还包括第三多通阀，所述第三多通阀同时管道连接多个所述测序试剂瓶，以及所述第二三通阀，所述第三注射泵经所述第二三通阀和所述第三多通阀与其中一个所述测序试剂瓶相接通。
  6. 根据权利要求5所述的基因测序的流体控制装置，其特征在于，所述测序试剂配置组件还包括冲洗试剂瓶和第一废液瓶，所述冲洗试剂瓶用于盛装冲洗试剂，所述冲洗试剂瓶经所述第二多通阀和所述第二三通阀与所述第三注射泵相接通，所述第一废液瓶用于盛装废液，所述第一废液瓶经所述第三多通阀和所述第二三通阀与所述第三注射泵相接通。
  7. 根据权利要求2所述的基因测序的流体控制装置，其特征在于，所述基因测序的流体控制装置还包括成像试剂配置组件，所述成像试剂配置组件包括多个成像试剂原料瓶、第四多通阀、第三三通阀和第四注射泵，多个所述成像试剂原料瓶用以盛装多种成像试剂原料，所述第四多通阀同时管道连接多个所述成像试剂原料瓶，以及管道连接所述第三三通阀，所述第三三通阀还管道连接所述第四注射泵和所述成像试剂瓶，所述第四注射泵经所述第三三通阀和所述第四多通阀与其中一个所述成像试剂原料瓶相接通，所述成像试剂瓶经所述第三三通阀与所述第四注射泵相接通，其中，所述第四注射泵依次与多个所述成像试剂原料瓶相接通，以抽取多个所述成像试剂原料瓶中的成像试剂原料，并混合配置成所述成像试剂，所述第四注射泵与所述成像试剂瓶相接通，用于将所述成像试剂注射至所述成像试剂瓶内。
  8. 根据权利要求7所述的基因测序的流体控制装置，其特征在于，所述成 像试剂配置组件还包括第二混合器，所述第二混合器连接于所述第三三通阀和所述成像试剂瓶之间，所述第二混合器包括多个第二蜿蜒管道，多个所述第二蜿蜒管道首尾相连，并接通于所述第三三通阀和所述成像试剂瓶之间。
  9. 根据权利要求1所述的基因测序的流体控制装置，其特征在于，所述驱动组件还包括第四三通阀、第五三通阀、第二废液瓶和第三废液瓶，所述第四三通阀管道连接于所述第一注射泵和所述第一基因测序通道之间，同时还管道连接所述第二废液瓶，所述第五三通阀管道连接于所述第二注射泵和所述第二基因测序通道之间，同时还管道连接所述第三废液瓶。
  10. 根据权利要求1所述的基因测序的流体控制装置，其特征在于，所述第一基因测序通道和所述第二基因测序通道的数目均为多个。
  11. 根据权利要求1所述的基因测序的流体控制装置，其特征在于，所述基因测序的流体控制装置还包括控制组件，所述控制组件电连接所述第一多通阀、所述第一三通阀、所述基因测序芯片和所述驱动组件，以控制所述第一多通阀、所述第一三通阀、所述基因测序芯片和所述驱动组件运行。
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