WO2017044651A2 - Oligonucléotides courts extincteurs pour réduire la fluorescence de ligne de base de sonde taqman - Google Patents

Oligonucléotides courts extincteurs pour réduire la fluorescence de ligne de base de sonde taqman Download PDF

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Publication number
WO2017044651A2
WO2017044651A2 PCT/US2016/050811 US2016050811W WO2017044651A2 WO 2017044651 A2 WO2017044651 A2 WO 2017044651A2 US 2016050811 W US2016050811 W US 2016050811W WO 2017044651 A2 WO2017044651 A2 WO 2017044651A2
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WO
WIPO (PCT)
Prior art keywords
oligonucleotide
quencher
oligonucleotide probe
linked
fluorophore
Prior art date
Application number
PCT/US2016/050811
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English (en)
Other versions
WO2017044651A3 (fr
Inventor
Erik Gustafson
Xin Zeng
Antonio Reyes
Jessica Smith
Original Assignee
Beckman Coulter, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Beckman Coulter, Inc. filed Critical Beckman Coulter, Inc.
Publication of WO2017044651A2 publication Critical patent/WO2017044651A2/fr
Publication of WO2017044651A3 publication Critical patent/WO2017044651A3/fr

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne des oligonucléotides extincteurs et des procédés pour utiliser ces oligonucléotides pour réduire une fluorescence de ligne de base et augmenter la sensibilité d'analyse pour des analyses PCR en temps réel. L'invention concerne également des kits qui peuvent être utilisés à cet effet.
PCT/US2016/050811 2015-09-10 2016-09-08 Oligonucléotides courts extincteurs pour réduire la fluorescence de ligne de base de sonde taqman WO2017044651A2 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562216794P 2015-09-10 2015-09-10
US62/216,794 2015-09-10

Publications (2)

Publication Number Publication Date
WO2017044651A2 true WO2017044651A2 (fr) 2017-03-16
WO2017044651A3 WO2017044651A3 (fr) 2017-04-06

Family

ID=56940442

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2016/050811 WO2017044651A2 (fr) 2015-09-10 2016-09-08 Oligonucléotides courts extincteurs pour réduire la fluorescence de ligne de base de sonde taqman

Country Status (1)

Country Link
WO (1) WO2017044651A2 (fr)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5723591A (en) 1994-11-16 1998-03-03 Perkin-Elmer Corporation Self-quenching fluorescence probe

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5455170A (en) * 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
JP4608483B2 (ja) * 2003-02-18 2011-01-12 アプライド バイオシステムズ， エルエルシー ポリヌクレオチドの複合的分析のための組成物および方法
WO2006045009A2 (fr) * 2004-10-20 2006-04-27 Stratagene California Compositions de sonde triplex et procedes de detection de polynucleotide
EP2116614A1 (fr) * 2008-05-06 2009-11-11 Qiagen GmbH Détection simultanée de plusieurs séquences d'acide nucléique dans une réaction
US9637790B2 (en) * 2010-12-03 2017-05-02 Brandeis University Detecting mutations in DNA

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5188934A (en) 1989-11-14 1993-02-23 Applied Biosystems, Inc. 4,7-dichlorofluorescein dyes as molecular probes
US5723591A (en) 1994-11-16 1998-03-03 Perkin-Elmer Corporation Self-quenching fluorescence probe

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HAUGLAND: "Handbook of Fluorescent Probes and Research Chemicals", 1989, MOLECULAR PROBES, INC.
KLENOW ET AL., EUR. J. BIOCHEM., vol. 22, 1971, pages 371
KLENOW ET AL., PROC. NATL. ACAD. SCI., vol. 65, 1970, pages 168

Also Published As

Publication number Publication date
WO2017044651A3 (fr) 2017-04-06

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