WO2017039279A1 - Module de prétraitement d'un échantillon et procédé de prétraitement d'un échantillon l'utilisant - Google Patents

Module de prétraitement d'un échantillon et procédé de prétraitement d'un échantillon l'utilisant Download PDF

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Publication number
WO2017039279A1
WO2017039279A1 PCT/KR2016/009640 KR2016009640W WO2017039279A1 WO 2017039279 A1 WO2017039279 A1 WO 2017039279A1 KR 2016009640 W KR2016009640 W KR 2016009640W WO 2017039279 A1 WO2017039279 A1 WO 2017039279A1
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WO
WIPO (PCT)
Prior art keywords
sample
chamber
dotting
sample pretreatment
discharge
Prior art date
Application number
PCT/KR2016/009640
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English (en)
Korean (ko)
Inventor
김유래
Original Assignee
주식회사 나노엔텍
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR1020160107601A external-priority patent/KR101808231B1/ko
Application filed by 주식회사 나노엔텍 filed Critical 주식회사 나노엔텍
Priority to JP2018530454A priority Critical patent/JP6606615B2/ja
Priority to CN201680050579.XA priority patent/CN107923824A/zh
Priority to EP16842245.9A priority patent/EP3346256B1/fr
Priority to US15/757,523 priority patent/US10758877B2/en
Publication of WO2017039279A1 publication Critical patent/WO2017039279A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • H—ELECTRICITY
    • H02—GENERATION; CONVERSION OR DISTRIBUTION OF ELECTRIC POWER
    • H02N—ELECTRIC MACHINES NOT OTHERWISE PROVIDED FOR
    • H02N11/00—Generators or motors not provided for elsewhere; Alleged perpetua mobilia obtained by electric or magnetic means

Definitions

  • the present invention relates to a sample pretreatment module and a sample pretreatment method using the same, and more particularly, to reduce the error of the operator in the sample pretreatment process, to ensure the reliability of the test results, and to perform the pretreatment process simply and easily.
  • the present invention relates to a sample pretreatment module capable of quantitative discharging of a sample and a sample pretreatment method using the same.
  • one of the important things in analyzing such a fluid sample is to pretreat the fluid sample.
  • the pretreatment of the fluid sample is to extract a desired amount of sample prior to analysis of the fluid sample and to accurately process it in an appropriate ratio, for example, in a dilution buffer, or to mix with a reaction reagent in solid or liquid state, or to fill or support the liquid sample.
  • an appropriate ratio for example, in a dilution buffer, or to mix with a reaction reagent in solid or liquid state, or to fill or support the liquid sample.
  • a pipette or dropper is used to pretreat the fluid sample, but in the analysis of samples in units of lab-on-a-chip or lab-on-a-tip, Since the amount of sample used is extremely small and must be processed very accurately, it is not easy for the operator to directly pretreat the desired small amount of sample accurately using a pipette or dropper.
  • a sample pretreatment module capable of minimizing an operator error in pretreatment of a very small amount of blood or other samples, easily and easily performing a pretreatment process, and capable of quantitatively discharging a pretreated sample.
  • Embodiments of the present invention are intended to minimize the errors that can occur when the operator proceeds by hand, and to ensure the accuracy and uniformity of the sample pretreatment and test results.
  • the pressure in the chamber is to be maintained and adjusted uniformly to prevent the sample from bursting under rapid pressure changes in the chamber.
  • the magnetic force is used to increase the mixing effect of the sample and to minimize mechanical driving.
  • the present invention provides a sample pretreatment module capable of quantitatively discharging the sample after pretreatment.
  • the body having a chamber for receiving a sample therein; A cap coupled to one end of the body; A dotting substrate, dotting a reagent in at least a portion, and inserting into the chamber; A discharge tip movably coupled to the other end of the body and discharging a sample received in the chamber; A permanent magnet inserted into the chamber and mixing the sample by rotating by a magnetic force acting according to a change in a magnetic field applied from the outside; And a moving part provided to be movable in the cap to pressurize the sample in the chamber and discharge the sample to the outside according to the movement thereof.
  • the dotting member may include a dotting member body, at least one first extension extending to one side of the dotting member body, and at least one second extension extending to the other side of the dotting member body. have.
  • the length of the entire dotting member may be configured to match the length of the chamber.
  • the permanent magnet may be magnetized to N-S or S-N along the vertical direction.
  • the cap may include a hollow portion in communication with the chamber, and the moving portion may be provided to be movable in the hollow portion.
  • the sample pretreatment module according to the present invention may be formed along the inner wall of the hollow portion, and may further include at least one bending line for adjusting the pressure in the chamber.
  • the sample pretreatment module includes a through membrane interposed between the chamber and the discharge tip, and is provided at one end of the chamber side of the discharge tip, and passes through the through membrane as the discharge tip moves to the chamber side, thereby allowing the sample in the chamber to pass through. It may further comprise a through part for forming a discharge passage capable of discharging the.
  • the sample pretreatment module according to the present invention may be formed on the through membrane, and may further include a through guide for guiding the through portion to penetrate a predetermined position of the through membrane.
  • the method comprises: dotting and drying a buffer in a chamber forming a predetermined space inside the body; Inserting a dotting member and a permanent magnet into the chamber, and coupling a discharge tip to the body; Injecting a sample into the chamber; Coupling a cap connected to one end of the body to close the chamber; And mixing the sample by rotating by applying a magnetic force to the permanent magnet in the chamber, the sample pretreatment method may be provided.
  • the sample pretreatment method according to the present invention further comprises the step of penetrating the through-film interposed between the chamber and the discharge tip through the through portion provided in the discharge tip, by moving the moving unit provided in the cap to discharge the sample quantitatively; Can be done.
  • the sample pretreatment method according to the present invention may further comprise the step of doping and drying the gold nanoparticles on one side of the dotting member, before inserting the dotting member in the chamber.
  • Embodiments of the present invention can minimize the errors that can occur when the operator proceeds by hand and ensure the accuracy and uniformity in the pre-treatment and test results of the sample.
  • the mixing and discharging of the sample can be easily performed to increase the convenience of work and to provide a user-friendly experimental environment.
  • the pressure in the chamber can be maintained and adjusted uniformly to prevent the sample from bursting even under a sudden pressure change in the chamber.
  • Magnetic force can also be used to increase the mixing effect of the sample and to minimize mechanical drive.
  • a sample pretreatment module capable of quantitatively discharging the sample after the pretreatment of the sample may be provided.
  • FIG. 1 is a perspective view of a sample pretreatment module according to an embodiment of the present invention.
  • FIG. 2 is a side view of a sample pretreatment module according to an embodiment of the present invention.
  • Figure 3 is an exploded perspective view partially cut into a sample pretreatment module according to an embodiment of the present invention
  • FIG. 4 is a perspective view and a front view showing a dotting member of the sample pretreatment module according to an embodiment of the present invention
  • FIG. 5 is a perspective view showing a body and a cap of a sample pretreatment module according to an embodiment of the present invention.
  • Figure 6 is a cross-sectional view showing the discharge tip of the sample pretreatment module according to an embodiment of the present invention
  • FIG. 7 is a configuration diagram illustrating a method for obtaining a diameter of an outlet for quantitative discharge of a sample pretreatment module according to an embodiment of the present invention.
  • FIG. 8 is a perspective view of a sample pretreatment system according to an embodiment of the invention.
  • FIG. 9 is a perspective view illustrating a state in which a sample pretreatment module is seated in a state in which a holder stage of a sample pretreatment system is withdrawn according to an embodiment of the present invention
  • FIG. 10 is a perspective view illustrating a module holder of a sample pretreatment system according to an embodiment of the present invention.
  • FIG. 11 is a perspective view showing a holder stage of the sample pretreatment system is withdrawn according to an embodiment of the present invention.
  • FIG. 12 is a perspective view illustrating a holder stage of a sample pretreatment system according to an exemplary embodiment of the present invention.
  • FIG. 13 is a diagram illustrating a process of doping and drying gold nanoparticles in a dotting member of a sample pretreatment module according to an exemplary embodiment of the present invention.
  • FIG. 14 is a block diagram illustrating a process of dotting and drying a buffer in a chamber of a sample pretreatment module according to an exemplary embodiment of the present invention.
  • 15 is a configuration diagram showing a state in which each part of the sample pretreatment module according to an embodiment of the present invention is assembled
  • 16 is a diagram illustrating a state in which a sample is injected into a chamber of a sample pretreatment module according to an embodiment of the present invention.
  • FIG. 17 is a block diagram illustrating a process of mixing a sample by applying magnetic force to a permanent magnet of a sample pretreatment module according to an embodiment of the present invention.
  • FIG. 18 is a block diagram illustrating a process of penetrating the through membrane by pressing the cap edge of the sample pretreatment module according to an embodiment of the present invention.
  • FIG. 19 is a block diagram illustrating a process of discharging a sample by pressing the moving part of the sample pretreatment module cap according to an embodiment of the present invention.
  • FIG. 1 is a perspective view of a sample pretreatment module according to an embodiment of the present invention
  • FIG. 2 is a side view of a sample pretreatment module according to an embodiment of the present invention
  • FIG. 3 is a sample according to an embodiment of the present invention.
  • Figure 4 is a perspective view and a front view showing a dotting member of the sample pretreatment module according to an embodiment of the present invention
  • Figure 5 is a perspective view showing a body and a cap of the sample pretreatment module according to an embodiment of the present invention
  • 6 is a cross-sectional view showing the discharge tip of the sample pretreatment module according to an embodiment of the present invention.
  • 7 is a configuration diagram illustrating a method of obtaining a diameter of an outlet for quantitative discharge of a sample pretreatment module according to an embodiment of the present invention.
  • the sample pretreatment module 100 includes a body 110 having a chamber 112 accommodating a sample therein and one end of the body 110.
  • the body 110 may be formed in a cylindrical shape having a predetermined height
  • the chamber 110 may be provided with a chamber 112 forming a predetermined space also made of a cylindrical shape.
  • the shape of the body 110 and the chamber 112 is not limited to a cylindrical shape, it may be modified in various forms as necessary.
  • the body 110 heats the sample accommodated in the chamber 112
  • One side of the body 110 may be provided with an inlet port 114 so that a sample or buffer may be injected into the chamber 112.
  • the dotting member 130 is also inserted into the chamber 112 through the inlet port 114. Can be accommodated.
  • the discharge tip 140 is coupled to the other side of the body 110, and a through film 116 may be provided between the chamber 112 and the discharge tip 140.
  • the through film 116 blocks the communication with the discharge tip 140 until it is penetrated by the through part 146, which will be described later, so that the sample may be accommodated in the chamber 112.
  • the through-membrane 116 is dried with a doped buffer applied to the chamber 112 before sample injection, and then the injected sample is mixed with the buffer to form a dilution or a mixed solution.
  • the pretreatment process is performed.
  • the pretreatment material may be applied not only to the through membrane 116 but also to the inside of the chamber 112 such as the inner wall of the chamber 112.
  • the body 110 and the discharge tip 140, the cap 120 and the through-membrane 116 may be made of a synthetic resin of elastic material, for example PS (Polystyrene), PP (Polypropylene) or PE (Polyethylene) ) And other elastic materials can also be used, and can be manufactured by injection molding using elastic materials.
  • PS Polystyrene
  • PP Polypropylene
  • PE Polyethylene
  • the resin material of the discharge tip 140 is made of PP. This is to consider the viscosity of the solution, because the pretreatment solution of Vitamin D is low viscosity to make the discharge tip 140 hydrophobic to prevent the sample from flowing out arbitrarily unintentionally and to be controlled by the quantitative discharge.
  • the discharge tip 140 may be made of a hydrophilic material so as to smoothly discharge the sample mixed with the buffer.
  • the dotting member 130 is accommodated in the chamber 112 together with the sample, and a predetermined reagent is inserted and inserted into at least one side of the dotting member 130 so that the received sample may react with or mix with the reagent.
  • the dotting member 130 may be doped with an additional sample or pretreatment material.
  • the dotting member 130 may include a dotting member body 134, at least one first extension part 136 extending to one side of the dotting member body 134, and the other side of the dotting member body 134. It may include at least one second extension portion 138 that is extended.
  • the dotting member body 134 has a substantially rectangular polyhedral shape, and the aforementioned reagent may be mainly doped with the second extension 138.
  • the present invention is not limited thereto, and reagents may also be inserted into the body 134 and the first extension 136 to be inserted into the chamber 112.
  • two first extension parts 136 may extend upward from both sides of the dotting member body 134.
  • the first extension part 136 is separated into two parts to form a sample injection space, and to provide a space for the pipette to enter the inlet of the chamber 112.
  • the dotting member 130 is designed to be in close contact with the outer wall of the chamber 112 as much as possible in order to be inserted fluidly even if an error occurs.
  • the second extension part 138 may extend downward from the lower portion of the dotting member body 134. In this case, the second extension part 138 may extend downward while forming a step with the dotting member body 134.
  • the reagent that is originally doped must be present in the area where the sample contacts, and when the reagent is dropped on the surface of the second extension 138, the reagent is diffused and dried, making it difficult to locally control the dotting area.
  • the reagent since a step is formed between the dotting member body 134 and the second extension 138, the reagent may be doped without spreading out of the step.
  • the length of the second extension 138 may be extended by (or less) the level of the sample contained in the chamber 112 to allow the sample and the reagent to fully react. That is, the area of the second extension 138 or the length of the extension may be adjusted according to the amount of sample to be injected.
  • the shape of the dotting member 130 is not limited to that shown in FIG. 4, and may be changed to various shapes as necessary.
  • the permanent magnet 132 together with the dotting member 130 may be inserted together into the chamber 112.
  • the permanent magnet 132 is formed in a cylindrical shape, and serves to mix the sample by rotating by a magnetic force acting according to a change in the magnetic field applied from the outside.
  • the permanent magnet 132 may be seated in the space formed by the second extension 138 to mix the sample.
  • the permanent magnet 132 is magnetized to NS or SN along an up and down direction, and when the vortexing magnet 320 (see FIG. 17) is rotated around the chamber 112, the permanent magnet 132 also follows the circumferential direction. Will rotate.
  • the rotation axis of the vortexing magnet 320 and the rotation axis of the permanent magnet 132 are perpendicular to each other. If the vortexing magnet 320 is located above or below the vertical direction of the sample pretreatment module 100, the axis of rotation of the vortexing magnet 320 and the axis of rotation of the permanent magnet 132 are horizontal to each other. do.
  • the second extension 138 of the dotting member 130 may be provided with additional samples by dotting and drying.
  • gold nanoparticles G, see FIG. 13
  • the second extension 138 may be provided with additional samples by dotting and drying.
  • various pretreatment materials may be used.
  • the method of applying the pretreatment material to the dotting member 130 may be selectively applied as well as the above-described dotting and drying methods.
  • the pretreatment material may be accommodated in the chamber 112, such as the through film 116 or the inner wall of the chamber 112, using the aforementioned method.
  • the length of the entire dotting member 130 may be configured to match the length of the chamber 112. Therefore, the dotting member 130 may also serve as a frame for maintaining the shape of the chamber 112 when inserted into the chamber 112.
  • the cap 120 may be coupled to the inlet 114 side of the body 110.
  • the cap 120 may be provided in a state of being connected to one side of the body 110 by a cap connecting portion 128.
  • the cap 120 is provided with a hollow portion 122 in communication with the chamber 112, and is movable in the hollow portion 122 to pressurize a sample in the chamber 112 according to the movement thereof to the outside. It may be made to include a moving part 124 to discharge.
  • the moving part 124 may be made of, for example, a rubber packing.
  • the hollow part 122 may be in communication with the chamber 112 when the cap 120 is coupled to the body 110.
  • the cap 120 includes a chamber communicating part 126 extending from the hollow part 122, and the chamber communicating part 126 is fitted into the inlet 114 so that the cap 120 is provided.
  • the chamber 112 and the hollow portion 122 may be in communication with each other while being coupled to the body 110.
  • the moving part 124 inserted into the hollow part 122 When the moving part 124 inserted into the hollow part 122 is pressed, the moving part 124 moves in the direction of the chamber 112, and pressure is transferred into the chamber 112 to discharge the sample to the outside. Can be.
  • the through membrane 116 since the chamber 112 is blocked by the through membrane 116, the through membrane 116 must be drilled first in order to discharge the pretreated sample. To this end, as the discharge tip 140 moves toward the chamber 112, the through part 146 penetrates the through membrane 116 to form a discharge passage 149 through which the sample in the chamber 112 can be discharged. ) May be provided.
  • the discharge tip 140 has a double-pointed structure in which the discharge part 142 on one side and the through part 146 on the other side are communicated by the discharge flow path 149.
  • the discharge tip 140 has an insertion body 144 is inserted into the insertion hole 118 formed in the body 110, the through portion 146 from the upper end of the insertion body 144 Is formed extending.
  • the penetrating portion 146 has a sharp tip shape so as to penetrate the penetrating membrane 116 while moving.
  • the through part 146 may be formed in a tapered shape with both sides symmetrically inclined so that the center part becomes sharp.
  • a locking jaw 119 having a convex shape along the circumferential direction is formed at an inner circumferential side of the insertion hole 118, and an upper tip of the insertion body 144 is caught by the locking jaw 119 during initial assembly.
  • a locking portion 145 may be formed to limit further movement of the 140.
  • the penetrating portion 146 penetrates the penetrating membrane 116 by additionally applying an external force.
  • a through guide 116a may be formed in the through film 116 to guide the through part 146 to pass through a predetermined position of the through film 116.
  • the through guide 116a guides the through part 146 so that the central part of the through film 116 can pass therethrough.
  • the through guide 116a has a possibility that the sample can leak through the through portion 146 through any position of the through membrane 116 when the body 110 and the discharge tip 140 is initially assembled It can also play a role in suppression.
  • the lower portion of the insertion body 144 has a flange portion 143 is formed. Since the flange portion 143 is caught by the edge of the insertion hole 118 of the body 110, the through film 116 may restrict the further advancement of the discharge tip 140 after passing through the through film 116. have.
  • a discharge passage 149 through which the sample in the chamber 112 is discharged is formed in the state where the through portion 146 of the discharge tip 140 passes through the through membrane 116.
  • the sample may be discharged through the discharge part 142.
  • the amount of sample to be discharged depends on the moving distance and the speed of the moving part 124, but the operator may press the moving part 124 directly, but by disposing and applying a device capable of applying a constant speed and distance.
  • the amount to be kept can be kept constant in quantitative terms.
  • the drop volume of the sample to be discharged may vary according to the size of the discharge unit 142, it is possible to adjust the volume discharged according to the diameter of the discharge unit 142.
  • the discharge part 142 may be formed to have a diameter corresponding to the type of the sample pretreated in the chamber 112 and the amount to be discharged.
  • the diameter of the discharge portion 142 may be obtained by the size of the spherical cap of the sphere.
  • the radius of the discharge portion 142 is a
  • the radius of the discharged sample droplet d is r
  • the height of the cut portion of the sphere is h
  • the through part 146 penetrates the through membrane 116, the pressure in the chamber 112 is rapidly increased, the sample contained in the chamber 112 may burst.
  • a pressure in the chamber 112 may increase.
  • a bending line 125 may be formed on the inner wall of the hollow part 122 of the cap 120 to adjust the pressure in the chamber 112.
  • the bending line 125 may be formed to have a predetermined length along a vertical direction on the inner wall of the hollow part 122, and serve to adjust the pressure in the chamber 112 by discharging the elevated pressure in the chamber 112 to the outside. have.
  • the two venting lines 125 are formed at one side and the other side of the inner wall of the hollow part 122, so that even when one venting line 125 is blocked, the venting line 125 continues to control the pressure in the chamber 112. This can be done.
  • FIG. 8 is a perspective view of a sample pretreatment system according to an embodiment of the present invention
  • FIG. 9 illustrates a state in which a sample pretreatment module is seated in a state where a holder stage of a sample pretreatment system according to an embodiment of the present invention is drawn out.
  • 10 is a perspective view of a module holder of a sample pretreatment system according to an embodiment of the present invention.
  • 11 is a perspective view illustrating a holder stage of a sample pretreatment system withdrawn according to an embodiment of the present invention
  • FIG. 12 illustrates a holder stage of a sample pretreatment system according to an embodiment of the present invention.
  • the sample pretreatment system 1000 includes a holder cashier 500 having a module holder 520 on which the above-described sample pretreatment module 100 is mounted, A cartridge accommodating part 400 in which a cartridge (not shown) into which the sample contained in the chamber 112 of the sample pretreatment module 100 is discharged and loaded is stored.
  • the permanent magnet provided in the sample pretreatment module 100 ( The magnetic force generating unit 300 to generate the magnetic force to rotate the 132 and the through-membrane 116 of the sample pretreatment module 100, and press the moving part 124 of the cap 120 to the sample It may be made to include a through and discharge execution unit 200 for discharging.
  • the holder cashier 500 serves to load the sample pretreatment module 100 containing the sample to be preprocessed into the sample pretreatment system 1000.
  • the internal configuration of the sample pretreatment system 1000 is illustrated to be exposed, but a cover (not shown) covering the outside may be provided.
  • the holder withdrawal unit 500 As described above is provided with a module holder 520 on which the sample pretreatment module 100 is seated.
  • the module holder 520 is installed on the holder stage 530, and the holder stage 530 is movable to move the module holder 520 to a loading or unloading position.
  • a fourth motor 510 may be provided at one side of the holder stage 530 to provide a driving force for moving the holder stage 530 together with the module holder 520.
  • the fourth motor 510 rotates the cashier pinion gear 512 connected to the rotating shaft of the motor, and the cashier pinion gear 512 is engaged with a rack gear (not shown) provided under the holder stage 530.
  • the holder stage 530 is moved in the horizontal direction by converting the rotational motion into the horizontal motion.
  • a guide rail 532 is provided below the holder stage 530 to guide horizontal movement of the holder stage 530.
  • the fourth motor 510 is driven in one direction so that the holder stage 530 is slid out on the guide rail 532.
  • a door (not shown) through which the holder stage 530 may enter or exit may be provided on the entire cover (not shown) of the sample pretreatment system 1000.
  • the fourth motor 510 is driven again in the opposite direction, so that the holder stage 530 is It slides on the guide rail 532 and is received inward.
  • This operation may be implemented such that the user presses a switch (not shown) provided on the outside.
  • the module holder 520 provided on the holder stage 530 forms a space having a substantially cylindrical shape inward to allow the sample pretreatment module 100 to be seated.
  • a module heater 524 for heating the sample pretreatment module 100 may be provided outside the module holder 520.
  • the module heater 524 is made of a heating wire surrounding the outside of the holder body 522, as shown in Figure 10, the sample pretreatment module seated inside the holder body 522 by the heat generated as the current flows The 100 is heated.
  • the body 110 of the sample pretreatment module 100 since the body 110 of the sample pretreatment module 100 according to the present embodiment has a thin thickness and has high heat transfer power, the body 110 absorbs heat emitted from the module heater 524 as a heat source, thereby desired.
  • the sample can be heated to temperature.
  • the holder body 522 interposed between the module heater 524 and the sample pretreatment module 100 is also made of a metallic material having good thermal conductivity.
  • the heater cover 526 is installed on the outside of the module heater 524 to finish.
  • the temperature and the retention time of the sample pretreatment module 100 may vary the types of samples and buffers that are subject to pretreatment. For example, vitamin D is maintained at 49 ° C for 10 minutes, and FreeT4 and Testosterone at 37 ° C for 5 minutes.
  • the through and discharge execution unit 200 presses the cap rim portion 129 (see FIGS. 18 and 19) of the sample pretreatment module 100 to pass through the membrane 116 inside the sample pretreatment module 100. Edge pressing portion 220 to penetrate) and the moving portion pressing portion 230 for pressing the moving portion 124 to discharge the sample may be made.
  • the through and discharge execution unit 200 may play a role of penetrating the through membrane 116 of the sample pretreatment module 100 described above and pressurizing the sample to be discharged. Can be.
  • the amount of sample discharged depends on the moving distance and the speed of the moving unit 124 of the sample preprocessing module 100, and the moving unit 124 is moved at a constant speed through the moving unit pressing unit 230.
  • the amount of discharged can be kept constant in a fixed amount by pressurizing with a distance.
  • One side of the module holder 520 may be provided with a magnetic force generating unit 300 for generating a magnetic force to rotate the permanent magnet 132 provided in the sample pretreatment module 100.
  • the magnetic force generating unit 300 includes a vortexing magnet 320 rotatably installed at one side of the module holder 520 and a second motor 310 for rotating the vortexing magnet 320. It is provided.
  • the cartridge accommodating unit 400 serves to load or unload a cartridge (not shown) into which a preprocessed sample is to be inserted, and has a third motor 410 that provides a driving force for loading or unloading. .
  • FIG. 13 is a diagram illustrating a process of doping and drying gold nanoparticles in a dotting member of a sample pretreatment module according to an embodiment of the present invention
  • FIG. 14 is a view illustrating a sample pretreatment module according to an embodiment of the present invention
  • FIG. 15 is a diagram illustrating a process of dotting and drying a buffer in a chamber
  • FIG. 15 is a diagram illustrating a state in which each part of the sample pretreatment module according to an embodiment of the present invention is assembled.
  • 16 is a diagram illustrating a state in which a sample is injected into a chamber of a sample pretreatment module according to an embodiment of the present invention
  • FIG. 17 is a magnetic force applied to a permanent magnet of the sample pretreatment module according to an embodiment of the present invention.
  • 18 is a diagram illustrating a process of mixing a sample
  • FIG. 18 is a diagram illustrating a process of pressing a cap edge of a sample pretreatment module according to an embodiment of the present invention and penetrating the through membrane.
  • 19 is a block diagram showing a process of discharging a sample by pressing the moving part of the sample pretreatment module cap according to an embodiment of the present invention.
  • an additional sample is doped into the second extension 138 of the dotting member 130 to be inserted into the sample pretreatment module 100.
  • gold nanoparticles G are doped and dried in the second extension part 138.
  • a release buffer B is dripped and dried on the through membrane 116 in the chamber 112.
  • vitamin D may be used as a buffer.
  • each part of the sample pretreatment module 100 is assembled. That is, the permanent magnet 132 and the dotting member 130 are disposed in the chamber 112, and the discharge tip 140 is coupled to the body 110. At this time, the discharge tip 140 is a state in which only a part of the penetration portion 146 is inserted so as not to penetrate the through-membrane 116. Then, the moving part 124 is inserted into the hollow part 122 of the cap 120.
  • the sample is injected into the chamber 112, the cap 120 is closed, and the sample pretreatment module 100 is seated and loaded in the module holder 520 of the sample pretreatment system 1000.
  • the sample pretreatment module 100 is loaded into the sample pretreatment system 1000 as described above, when the vortexing magnet 320 is rotated as shown in FIG. 17, the magnetic force acts on the permanent magnet 132 to rotate. Mixing of the samples can be made.
  • the module heater 524 is operated to heat the sample. Specifically, for example, the applied temperature is heated at 49 ° C. for about 10 minutes in vitamin D mode, and at about 5 minutes at 37 ° C. in Free T4 and testosterone. .
  • the edge pressing portion 220 presses the cap edge portion 129 downward to penetrate the through membrane 116.
  • the moving part pressing part 230 moves downward, as shown in FIG. 19, and presses the moving part 124 located in the hollow part 122 of the cap 120. .
  • the moving part 124 When the moving part 124 is pressed downward while moving, the sample in the chamber 112 is discharged quantitatively through the discharge part 142. At this time, since the moving unit 124 is pressed by the moving unit pressing unit 230 at a constant moving distance and speed, the amount of discharged can be constantly maintained in a fixed amount.
  • the sample thus discharged is dropped into a fluid analysis cartridge (not shown) located at the bottom and used for diagnosis and analysis.
  • Table 1 and Table 2 are the results of the actual pretreatment and quantitative discharging experiment using the sample pretreatment module 100 of the present invention, the plasma and gold particles were mixed and maintained at 37 °C for 5 minutes.
  • the module of (4) is heated to 37 ° C. for 5 minutes in a pretreatment system unit.
  • the sample pretreatment module according to the embodiments of the present invention described so far can minimize errors that can occur when the operator proceeds by hand, and ensure the accuracy and uniformity of the sample pretreatment and test results.
  • the mixing and discharging of samples can be easily performed to increase the convenience of work and to provide a user-friendly experimental environment.
  • the pressure in the chamber can be maintained and adjusted uniformly to prevent the sample from bursting under rapid pressure changes in the chamber, and the sample can be mixed by heating to a desired temperature within a short time by increasing the heat transfer power of the sample contained in the chamber. And reaction efficiency can be improved.
  • the magnetic force may be used to increase the mixing effect of the sample and to minimize mechanical driving, and to discharge the sample after the pretreatment of the sample.

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  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne un module de prétraitement d'un échantillon et un procédé de prétraitement d'un échantillon l'utilisant. Le module de prétraitement d'un échantillon et le procédé de prétraitement d'un échantillon l'utilisant selon l'invention peuvent réduire au minimum les erreurs susceptibles de se produire quand un opérateur travaille manuellement, garantir la précision et l'uniformité du prétraitement de l'échantillon et des résultats des tests et, en permettant un mélange et une évacuation simple de l'échantillon, améliorer la praticité du travail et créer un environnement de tests convivial. De plus, le module et le procédé peuvent contrôler et maintenir une pression uniforme dans la chambre de façon à prévenir tout risque de déversement accidentel d'un échantillon, même en cas de très forte variation de pression dans la chambre, et peuvent améliorer le transfert de chaleur à l'échantillon se trouvant dans la chambre, chauffant ainsi l'échantillon jusqu'à la température recherchée en un court laps de temps et améliorant ainsi les efficacités de mélange et de réaction de l'échantillon. Le module et le procédé utilisent en outre une force magnétique, qui permet d'obtenir un mélange plus efficace de l'échantillon moyennant une opération mécanique minimale, et permettent la libération d'une quantité exacte de l'échantillon après son prétraitement.
PCT/KR2016/009640 2015-09-04 2016-08-30 Module de prétraitement d'un échantillon et procédé de prétraitement d'un échantillon l'utilisant WO2017039279A1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
JP2018530454A JP6606615B2 (ja) 2015-09-04 2016-08-30 サンプル前処理モジュール及びこれを用いたサンプル前処理方法
CN201680050579.XA CN107923824A (zh) 2015-09-04 2016-08-30 样品前处理模块及利用其的样品前处理方法
EP16842245.9A EP3346256B1 (fr) 2015-09-04 2016-08-30 Module de prétraitement d'un échantillon
US15/757,523 US10758877B2 (en) 2015-09-04 2016-08-30 Sample pretreatment module and pretreatment method using the same

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
KR10-2015-0125864 2015-09-04
KR20150125864 2015-09-04
KR1020160107601A KR101808231B1 (ko) 2015-09-04 2016-08-24 샘플 전처리 모듈 및 이를 이용한 샘플 전처리 방법
KR10-2016-0107601 2016-08-24

Publications (1)

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WO2017039279A1 true WO2017039279A1 (fr) 2017-03-09

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JPH0961425A (ja) * 1995-08-28 1997-03-07 Sekisui Chem Co Ltd 便潜血判定装置
JPH10170510A (ja) * 1996-12-12 1998-06-26 Fujirebio Inc 便潜血採取用具
KR20060005390A (ko) * 2003-04-25 2006-01-17 세키스이가가쿠 고교가부시키가이샤 검체 채취용 용기를 이용한 검체 여과 방법, 치구 및 검체채취용 용기
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* Cited by examiner, † Cited by third party
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CN108884110B (zh) * 2016-06-29 2021-09-14 三亚普罗股份有限公司 锍盐、光酸产生剂、光固化性组合物及其固化体

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