WO2017014340A1 - Composition containing stephanandra incisa (thunb.) zabel extracts having anti-aging efficacy and use containing same as active ingredient - Google Patents

Composition containing stephanandra incisa (thunb.) zabel extracts having anti-aging efficacy and use containing same as active ingredient Download PDF

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WO2017014340A1
WO2017014340A1 PCT/KR2015/007654 KR2015007654W WO2017014340A1 WO 2017014340 A1 WO2017014340 A1 WO 2017014340A1 KR 2015007654 W KR2015007654 W KR 2015007654W WO 2017014340 A1 WO2017014340 A1 WO 2017014340A1
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extract
noodle
active ingredient
aging
composition
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PCT/KR2015/007654
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French (fr)
Korean (ko)
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정현정
박성하
김유아
박병준
정태화
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한국콜마주식회사
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9783—Angiosperms [Magnoliophyta]
    • A61K8/9789—Magnoliopsida [dicotyledons]
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P39/00—General protective or antinoxious agents
    • A61P39/06—Free radical scavengers or antioxidants

Definitions

  • the present invention relates to a composition containing an extract of Noodles having an anti-aging effect and a use thereof as an active ingredient, and more particularly, to inhibiting NO production after treating lipopolysaccharide (LPS) that induces an inflammatory response.
  • LPS lipopolysaccharide
  • COX-2 cyclooxygenase-2
  • iNOS nitric oxide synthase
  • the aging process can be largely divided into intrinsic aging and photoaging. As the age increases, active oxygen species can be activated. If this condition is exacerbated, the function of cells and tissues is impaired, leading to aging. At this time, as a cause of inflammation, reactive oxygen species are produced by various biochemical stimuli in the body due to excessive stress, etc., various biochemical phenomena are involved as a cause of inflammation.
  • Inflammation is a local physiological response that protects the living body from factors that may or may not be harmful to tissue.
  • Enzymes such as elastase, hyaluronidase and lipoxygenase are well known for protein and lipolytic enzymes produced during inflammation. Their actions sometimes cause detrimental damage to adjacent tissue cells and noncellular components.
  • proteolytic enzymes caused by excessive inflammation damage cells and connective tissues, and the damage of connective tissues reduces skin elasticity and causes wrinkles, as well as rapid skin aging. Therefore, by inhibiting the following proteins and lipolytic enzymes produced by excessive inflammation, it is possible to protect the damaged cells thereby inhibiting skin aging.
  • ROS reactive oxygen species
  • NO nitric oxide
  • Cox-2 cyclooxygenase-2
  • nitric oxide is produced by nitric oxide synthas (NOS), and enzymes involved in nitric oxide synthas (NOS) are classified into two types.
  • NOS nitric oxide synthases
  • nNOS inducible nitric oxide synthases
  • eNOS endothelial nitric oxide synthase
  • LPS lipopolysaccharide -induced cytokines.
  • COX-2 which forms the central axis of the inflammatory mediator, is involved in the production of PGs (Prostaglandin).
  • COX-2 When COX-2 is activated, PGE 2 (Prostaglandin E 2 ) is produced. NF- ⁇ B is activated and moves to the nucleus to induce an inflammatory response and is involved in the expression of various genes such as iNOS and COX-2.
  • PGE 2 Prostaglandin E 2
  • NF- ⁇ B is activated and moves to the nucleus to induce an inflammatory response and is involved in the expression of various genes such as iNOS and COX-2.
  • Noodle Tree ( Stephanandra incisa ( Thunb.) Zabel is a deciduous broad-leaved shrub of the family Rosaceae, native to Korea, and distributed in Korea, northeastern China, Taiwan (Taiwan), and Japan. Inhabits forests or edges with mixed conifers and hardwoods, sunny slopes and roadsides. Likes the sunny side but grows well in the shade and is strong against cold. Its height is 1 ⁇ 2m, many branches come out, and it is limp on the ground like a long vine, so the whole is like a round bush. Twigs are round and have hairs or cilia.
  • the leaves are 2 ⁇ 5cm long, triangular broad egg-shaped, pointed at the end, sharp and deep teeth at the edges, and may be divided into several branches. Reddish petiole has fine hairs. Flowers bloom from May to June with yellowish white flowers with a diameter of 4-5mm and run in cones at the end of the branches. Calyx leaves are sharp at the end, 5 petals, 10 stamens, shorter than petals. Fruits are round or egg-shaped, with a lot of fine hairs and ripen in August-September.
  • Korean Patent No. 523443 discloses a composition for the prevention and / or treatment of obesity, based on one or more extracts selected from the group consisting of alder, cypress, and noodle from the native plant group, and Republic of Korea Patent Publication No. 2011-0068923 The issue reports a Noodle Leaf Extract as an active ingredient of an antihistamine composition.
  • US Patent Publication No. 2009-68291 discloses a Noodle Extract as a plant extract comprising an in vitro protease inhibitor and reports its activity.
  • the present inventors have investigated the physiological activity of domestic native plants, and the extract of N. japonica ( Stephanandra incisa ( Thunb.) Zabel) was treated with lipopolysaccharide (LPS), which induces an inflammatory response, and inhibited NO production.
  • LPS lipopolysaccharide
  • COX-2 Xenase-2
  • iNOS nitric oxide synthase
  • Another object of the present invention to provide a skin anti-aging cosmetic composition containing the noodle extract as an active ingredient.
  • Still another object of the present invention is to provide an external preparation composition for skin containing the noodle extract as an active ingredient.
  • the present invention has an anti-aging effect by the immune activity of the production inhibitory to inflammatory pathogens ( Stephanandra) incisa (Thunb.) Zabel) Provides a composition containing an extract.
  • Noodle extract extracts inhibited NO production and inhibited the production of cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS). Since it has an anti-aging or skin useful effect.
  • LPS lipopolysaccharide
  • COX-2 cyclooxygenase-2
  • iNOS nitric oxide synthase
  • the noodle extract is extracted from any one or two or more mixed sites selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and starch extracts of the Noodle plant, preferably water, hexane, methanol, It is extracted by an extraction solvent alone or in a mixture thereof selected from the group consisting of ethanol and butylene glycol.
  • the present invention provides a skin anti-aging cosmetic composition containing more than 0.001% by weight of the noodle extract as an active ingredient.
  • the present invention also provides a topical skin composition containing more than 0.001% by weight of noodle extract as an active ingredient.
  • Noodle extract of the present invention has a production inhibitory activity against inflammatory pathogens that increase in production as aging progress, specifically after the LPS treatment to induce an inflammatory response, NO production inhibition by the immune response, cyclooxygenase- Noodle tree effective for anti-inflammatory and anti-aging applications by confirming its excellent stability due to its anti-aging effect showing inhibitory activity against 2 (COX-2) and nitric oxide synthase (iNOS) expression and no toxicity and irritation to skin
  • COX-2 inhibitory activity against 2
  • iNOS nitric oxide synthase
  • the present invention can provide a skin anti-aging cosmetic composition and an external composition for skin by containing the extract of the tree as an active ingredient.
  • Figure 3 is a result showing the inhibitory effect on the expression of COX-2 and iNOS in LPS-activated mouse macrophages (Raw264.7) according to the extract of the Noodle Tree of the present invention.
  • the present invention has an anti-aging effect by the immune activity of the production inhibitory to inflammatory pathogens ( Stephanandra) incisa (Thunb.) Zabel) Provides a composition containing an extract.
  • the noodle extract of the present invention may include all the noodle parts, for example, any one selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and starch extracts of the noodle tree plant or It can be extracted from two or more mixed sites. More preferably, in the embodiment of the present invention, the extract extracted from the mixed form of the leaves and stems of the noodle tree implements more excellent immunological activity against the inflammatory invention factor, but is not limited thereto.
  • each extract may be extracted using an extraction solvent of a single or mixed form selected from the group consisting of water, hexane, methanol, ethanol and butylene glycol, preferably methanol extract In the case of excellent activity.
  • the extraction solvent of alcohols including methanol and alcohol may be used without particular limitation on the purity, but preferably 80 to 95% methanol and 70 to 80% ethanol.
  • the noodle extract of the present invention is heated to extract hot water by heating at 45 ⁇ 120 °C for 30 minutes to 24 hours using the above extraction solvent, or in a method of extracting shaking by immersion at 5 ⁇ 40 °C for 30 minutes to 15 days It can be prepared by at least one extraction method selected, it is possible to improve the yield of the extract according to the extraction solvent and the extraction method.
  • the amount of the extraction solvent is not particularly limited, but when used in excess of the plant material, the extract cannot be extracted sufficiently, it takes a lot of time and money to remove the extraction solvent and obtain a dry extract after extraction. Therefore, in the case of hot water extraction, it is preferable to include a sufficient amount of plant material, and to extract it in a heated state in order to increase the extraction efficiency. If the temperature is too low, it is difficult to fully utilize the benefits of warming and must be extracted for a long time. Higher levels may lead to degradation of useful substances.
  • the powdered sample is immersed in a sufficient extraction solvent for a sufficient time, preferably for about 3 days to ensure that the effective ingredient is extracted sufficiently, if the extraction is not sufficient
  • the extraction volume may be increased by an iterative process or by increasing the extraction time.
  • Noodle extract extracted from each of the above extraction solvents is not useful for skin application because no cytotoxicity was observed under the concentration condition of 100 ⁇ g / ml [ FIG. 1 ].
  • each noodle extract 100 ⁇ g / ml compared to the control group treated with LPS It is possible to confirm the NO production inhibitory effect at a level of 30 to 40% at the concentration [ FIG. 2 ].
  • the noodle extract of the present invention extracts COX-2 and iNOS inhibitory effect in a concentration-dependent manner. Indicates. In particular, as confirmed in Figure 3 , it can be confirmed that the result of the iNOS expression is suppressed at a concentration of 100 ⁇ g / ml.
  • the Noodle extract of the present invention has an effect of inhibiting NO production and COX-2, iNOS expression after LPS treatment to induce an inflammatory response, and toxic and irritating to the skin at the concentration of 100 ⁇ g / ml or less Since it is excellent in terms of stability, it can be contained as an active ingredient of a skin anti-aging cosmetic composition or a topical skin composition.
  • the present invention provides a skin anti-aging cosmetic composition containing the noodle extract as an active ingredient.
  • the present invention provides an external skin composition having an anti-inflammatory and anti-aging effect containing the noodle extract as an active ingredient.
  • the external skin composition is a cream, gel, patch, spray, ointment, warning, It may be applied to lotion, liniments, pasta or cataplasma formulations.
  • noodle extract is preferably contained as 0.001% by weight or more as an active ingredient, the content is selected as the minimum content to implement the production inhibitory activity against the inflammatory disease.
  • the effective content may be increased in consideration of stability and immune activity of noodle extract, but is preferably contained within 30% by weight in consideration of discoloration and deodorization when applying skin.
  • Noodle Tree ( Stephanandra incisa (Thunb.) Zabel)
  • a mixture of leaf, stem extract and water in a 1:20 weight ratio was extracted with hot water, filtered through a 380 mesh, and then re-filtered with a 0.45 ⁇ m membrane filter (Supor®-450, 60173, PALL Life Sciences). Filtered. Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare noodle leaves and stem extracts 5.8 g.
  • Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Leaves, stem extract and hexane was mixed in a 1:20 weight ratio at room temperature immersion for 3 days, the process was extracted three times, and then filtered through a 380 mesh 0.45 ⁇ m membrane filter (Supor® -450, 60173, PALL Life Sciences). Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare 6.4 g of noodle extract.
  • Noodles Stephanandra incisa (Thunb.) Zabel
  • Leaves, stem extracts and methanol were mixed in a 1:20 weight ratio at room temperature for 3 days, and the process was repeated three times, followed by extraction with a 380 mesh to filter 0.45 ⁇ m membrane filter (Supor® -450, 60173, PALL Life Sciences). Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare ethanol methanol extract 6.8 g.
  • Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Leaves, stem extracts and ethanol were mixed at 1:20 weight ratio to immerse at room temperature for 3 days, extracted three times, and then filtered through a 380 mesh 0.45 ⁇ m membrane filter (Supor®). -450, 60173, PALL Life Sciences). Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare 5.1 g of noodle ethanol extract.
  • Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) leaves, stem extract ingredients and butylene glycol were mixed in a 1:20 weight ratio, immersed at room temperature for 3 days, the process was extracted three times, and then filtered through a 380 mesh to 0.45 ⁇ m membrane filter Refiltration was performed to prepare a noodle butylene glycol extract.
  • Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Except for using the fruit component, was carried out in the same manner as in Example 3, to prepare a 5.7 g of Noodle fruit methanol extract.
  • Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Except for using the root component, it was carried out in the same manner as in Example 3, to prepare 4.2g of Noodle root methanol extract.
  • Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Except for using the starch component, it was carried out in the same manner as in Example 3, to prepare 6.3g of methanol extract Noodle outpost.
  • the noodle methanol extract prepared in Example 3 above was sampled in a 96-well plate at a concentration of 100, 75, 50, 25, 10, 5, 1, 0.5, 0.1 ⁇ g / ml in a 37 ° C., CO 2 incubator. Incubated for 24 hours.
  • mice macrophage Raw264.7 cells were injected into 96-well plates at a concentration of 5 ⁇ 10 cells / ml and 37 ⁇ l. C, incubated for 24 hours in a CO 2 incubator. In this case, DMEM / high glucose (Dulbeco's Modified Eagle Medium, Hyclone) containing 10% bovine serum and 1% antibiotic was used.
  • DMEM / high glucose Dulbeco's Modified Eagle Medium, Hyclone
  • Methanol extract prepared above was sampled in a 96-well plate at a concentration of 100, 50 or 25 ⁇ g / ml, and 90 ⁇ l of DMEM (Dulbecco's modified Eagle's medium) and 10 ⁇ l of sample at 37 ° C. in a CO 2 incubator. After the reaction, LPS (Lipopolysaccharide) was treated with 1 ⁇ g / ml and incubated for 24 hours at 37 °C, CO 2 incubator.
  • the amount of NO (Nitrite Oxide) produced was measured in the form of NO2 present in the cell culture solution using a Greries reagent (NED solution, sulfanilamide solution).
  • NED solution, sulfanilamide solution Cell culture supernatant 50 ⁇ l and Greece reagent (NED solution, sulfanilamide solution), respectively after the treatment by 50 ⁇ l reacted by blocking the light for 10 minutes at room temperature, and measuring the 540nm / 655nm absorbance, to the results in Table 1 and Table 2 is described.
  • RAW 264.7 cells (5 ⁇ 10 5 cells / ml) were pre-incubated for 18 hours, and the test substance (100, 50, 25 ⁇ g / ml of methanol extract of Example 3) and LPS (1 ⁇ g / ml) simultaneously Treated and incubated for 24 hours.
  • Formulation examples for the flexible cosmetics in the cosmetics containing the methanol extract of the noodle tree prepared in Example 3 as an active ingredient is shown in Table 3 below.
  • sodium hyaluronate was prepared as a 1% solution by dispersing at 3000 rpm propeller mixer in purified water.
  • the raw materials 1 to 8 were homogenized at 500 rpm in a propeller mixer in a water phase dissolving tank, warmed at 75 ° C. to completely dissolve, and then cooled to room temperature.
  • the raw materials 9-11 were completely melt
  • noodle methanol extract prepared in Example 3 was added and sufficiently stirred and mixed to prepare a flexible cosmetic water.
  • Formulation example for the nutrient cosmetic water in the cosmetics containing the methanol extract of noodle tree prepared in Example 3 as an active ingredient is shown in Table 4 .
  • Carbomer was prepared in 2% solution by dispersing with propeller mixer 4000rpm in purified water.
  • the raw materials 1-6 were thrown into the water melting tank, and it stirred at 2000 rpm and disperse
  • the raw materials 7-13 were put into the oil phase dissolution tank, and it melt
  • the oil phase dissolved in the aqueous phase dissolution tank was introduced and emulsified (3000 rpm / 5 minutes), followed by cooling to room temperature. Into this, noodle methanol extract prepared in Example 3 was added and sufficiently stirred and mixed to prepare nutritious longevity.
  • Formulation example for the essence of the cosmetics containing the methanol extract of noodle tree prepared in Example 3 as an active ingredient is shown in Table 5 below.
  • Sodium hyaluronate and hydroxyethyl cellulose were each dispersed in purified water at 2000 rpm with a propeller mixer to prepare a 1% solution.
  • Carbomer was prepared in 2% solution by dispersing with propeller mixer 4000rpm in purified water.
  • the raw materials 1-12 were thrown into the aqueous phase dissolution tank, and it stirred at 2000 rpm and disperse
  • the warm water phase was cooled to room temperature. After completely dissolving the raw materials 13-15 in another dissolution tank, the solution was added to the aqueous phase dissolution tank and stirred and mixed.
  • Example 3 Here, the noodle methanol extract prepared in Example 3 was added thereto, followed by sufficiently stirring and mixing to prepare an essence.
  • Formulation example for the nourishing cream in the cosmetic composition containing the methanol extract of noodle tree prepared in Example 3 as an active ingredient is shown in Table 6 .
  • the raw materials 1-8 were thrown into the water melting tank, it stirred at 2000 rpm, and it disperse
  • Raw materials 9-16 were thrown into the oil phase dissolution tank, and it heated and melt
  • the oil phase dissolved in the aqueous phase bath was added to emulsify (3000 rpm / 10 minutes), and then cooled to room temperature. Into this, noodle methanol extract prepared in Example 3 was added and sufficiently stirred and mixed to prepare a nourishing cream.
  • the present invention through the study on the physiological activity of domestic native plants ( Stephanandra) By incisa ( Thunb.) Zabel extract confirmed the anti-aging effect by the immunosuppressive activity of the production inhibitory to inflammatory onset factors, to provide a composition containing the Noodle extract effective for anti-inflammatory and anti-aging applications.
  • the present invention provides a skin anti-aging cosmetic composition and a topical skin composition containing the extract as an active ingredient by checking the stability of the noodle extract does not have toxicity and irritation to the skin.

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Abstract

The present invention relates to a composition containing Stephanandra incisa (Thunb.) Zabel extracts having an anti-aging efficacy and a use containing the same as an active ingredient. The present invention provides Stephanandra incisa (Thunb.) Zabel extracts having an anti-aging effect by means of immunological activity due to NO production repression and production inhibition of cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS), after treating lipopolysaccharides (LPS) that induce an inflammatory response. Furthermore, the extracts have excellent stability due to no toxicity and irritation to the skin, as well as the anti-aging effect due to the immunological activity, and thus the present invention can provide a skin anti-aging cosmetic composition and a skin composition for external application containing the same as an active ingredient.

Description

항노화 효능을 가지는 국수나무 추출물을 함유하는 조성물 및 그를 유효성분으로 함유한 용도Composition containing noodle extract having anti-aging effect and use thereof as an active ingredient
본 발명은 항노화 효능을 가지는 국수나무 추출물을 함유하는 조성물 및 그를 유효성분으로 함유한 용도에 관한 것으로서, 더욱 상세하게는 염증반응을 유도하는 리포폴리싸카라이드(LPS)를 처리한 후 NO 생성저해, 사이클로옥시제나제-2(COX-2) 및 일산화질소합성효소(iNOS) 생성억제의 면역활성에 의한 항노화 효과를 가지는 조성물을 제공하고, 나아가 피부에 독성 및 자극이 없어 안정성이 우수하므로, 상기 국수나무 추출물을 함유하는 조성물 및 그를 유효성분으로 함유한 피부 항노화 화장료 조성물 및 피부외용제 조성물에 관한 것이다.The present invention relates to a composition containing an extract of Noodles having an anti-aging effect and a use thereof as an active ingredient, and more particularly, to inhibiting NO production after treating lipopolysaccharide (LPS) that induces an inflammatory response. , To provide a composition having an anti-aging effect by the immunological activity of cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS) production inhibitory, and furthermore, it is excellent in stability because there is no toxicity and irritation to the skin, It relates to a composition containing the noodle extract and a skin anti-aging cosmetic composition and an external preparation composition containing the same as an active ingredient.
노화 과정은 크게 내적인 노화(intrinsic aging)와 광노화(photo-aging)로 나눌 수 있다. 연령증가에 따라 활성 산소종이 활성화될 수 있으며, 이런 상태가 심화될 경우 세포와 조직의 기능이 손상되어 결국 노화로 이어진다. 이때, 염증의 발생 원인으로 활성산소종은 과도한 스트레스 등에 의한 체내에서 다양한 생화학적 자극에 의하여 생성이 되며, 염증의 발생 원인으로는 다양한 생화학적 현상이 관여한다.The aging process can be largely divided into intrinsic aging and photoaging. As the age increases, active oxygen species can be activated. If this condition is exacerbated, the function of cells and tissues is impaired, leading to aging. At this time, as a cause of inflammation, reactive oxygen species are produced by various biochemical stimuli in the body due to excessive stress, etc., various biochemical phenomena are involved as a cause of inflammation.
염증은 조직에 해롭거나 해로울 가능성이 있는 요인들로부터 생체를 보호하는 국소적인 생리적 반응이다. 엘라스타제(Elastase), 히알루로니다아제(hyaluronidase) 및 리폭시게나아제(lipoxygenase)와 같은 효소들은 염증 시 생성되는 단백질 및 지질 분해 효소들로 잘 알려져 있다. 이들의 작용은 때로는 인접해 있는 조직 세포와 비세포 성분들에게도 해로운 손상을 일으키기도 한다. Inflammation is a local physiological response that protects the living body from factors that may or may not be harmful to tissue. Enzymes such as elastase, hyaluronidase and lipoxygenase are well known for protein and lipolytic enzymes produced during inflammation. Their actions sometimes cause detrimental damage to adjacent tissue cells and noncellular components.
따라서 적절한 조건하에서는 염증을 자극하는 자극제가 없어지지 않거나 계속해서 만들어지면 결과적으로 만성염증이 일어나게 되어서 더욱더 심각한 조직손상을 가져온다. Therefore, under the right conditions, if the irritant that stimulates inflammation does not go away or is made continuously, chronic inflammation can result, resulting in even more severe tissue damage.
이처럼 과다 염증에 의해 유발되는 단백질 분해효소들에 의해 세포 및 결합조직이 손상을 입고 결합조직의 손상은 피부탄력을 감소시켜 주름 원인이 될 뿐 아니라 나아가 빠른 피부 노화를 초래하게 된다. 그러므로 과다 염증에 의해 생성되는 하기의 단백질 및 지질분해효소들을 억제함으로써 그로 인해 손상되는 세포들을 보호하여 피부 노화를 억제할 수 있다. The proteolytic enzymes caused by excessive inflammation damage cells and connective tissues, and the damage of connective tissues reduces skin elasticity and causes wrinkles, as well as rapid skin aging. Therefore, by inhibiting the following proteins and lipolytic enzymes produced by excessive inflammation, it is possible to protect the damaged cells thereby inhibiting skin aging.
염증은 ROS(Reactive oxygen species), NO(nitric oxide), Cox-2(cyclooxygenase-2)와 같은 인자에 의해 유발된다. 상기 ROS는 세포내의 신호전달로에서 활성화가 이루어진다. Inflammation is caused by factors such as reactive oxygen species (ROS), nitric oxide (NO), and cyclooxygenase-2 (Cox-2). The ROS is activated in an intracellular signaling pathway.
또한, 상기 NO(nitric oxide)는 NOS(nitric oxide synthas)에 의해 생산이 되며, NOS(nitric oxide synthas)에 관여하는 효소는 두 가지로 분류된다. 세포 내에 항상 존재하는 nNOS(neuronal nitric oxide synthase)와 eNOS(endothelial nitric oxide synthase)와 LPS(lipopolysaccharide) 염증유도 사이토카인에 노출되는 경우에만 발현이 가능한 유도성 iNOS(inducible nitric oxide synthase)가 있다. 염증 매개체의 중심축을 이루는 COX-2는 PGs(Prostaglandin) 생산에 관여하며, COX-2가 활성화되면 PGE2(Prostaglandin E2)가 많이 생산된다. NF-κB가 활성화되어 핵으로 이동하여 염증반응을 유도하여 iNOS 및 COX-2 등의 다양한 유전자 발현에 관여한다. In addition, the nitric oxide (NO) is produced by nitric oxide synthas (NOS), and enzymes involved in nitric oxide synthas (NOS) are classified into two types. There are inducible nitric oxide synthases (nNOS), inducible nitric oxide synthases that can be expressed only when exposed to inflammatory cytokines such as endothelial nitric oxide synthase (nNOS), endothelial nitric oxide synthase (eNOS), and lipopolysaccharide (LPS) -induced cytokines. COX-2, which forms the central axis of the inflammatory mediator, is involved in the production of PGs (Prostaglandin). When COX-2 is activated, PGE 2 (Prostaglandin E 2 ) is produced. NF-κB is activated and moves to the nucleus to induce an inflammatory response and is involved in the expression of various genes such as iNOS and COX-2.
국수나무(Stephanandra incisa (Thunb.) Zabel)는 장미과의 낙엽활엽관목이며 한국이 원산지이고, 한국, 중국 동북부, 대만(타이완), 일본에 분포한다. 침엽수와 활엽수가 섞여 있는 숲 또는 가장자리, 빛이 잘 드는 경사 지대, 길가에 서식한다. 양지쪽을 좋아하나 음지에서도 잘 자라며 추위에 강하다. 높이가 1∼2m이며, 가지가 많이 나오며 긴 덩굴처럼 땅 위로 축축 늘어져서 전체가 둥그스름한 덤불처럼 된다. 잔가지는 둥글고 잔털 또는 선모가 있다. 잎의 길이는 2∼5cm로 어긋나고 세모진 넓은 달걀 모양이며 끝이 뾰족하며 가장자리에 날카롭고 깊은 톱니가 있으며 몇 갈래로 깊이 갈라지기도 한다. 붉은빛이 도는 잎자루는 잔털이 있으며, 꽃은5∼6월에 지름 4∼5mm의 노란색 빛이 도는 흰색 꽃이 햇가지 끝에 원추꽃차례로 달린다. 꽃받침 잎은 끝이 날카로우며, 꽃잎은 5개이고 수술은 10개로 꽃잎보다 짧다. 열매는 둥글거나 달걀 모양이고 잔털이 많으며 8∼9월에 익는다. Noodle Tree ( Stephanandra incisa ( Thunb.) Zabel is a deciduous broad-leaved shrub of the family Rosaceae, native to Korea, and distributed in Korea, northeastern China, Taiwan (Taiwan), and Japan. Inhabits forests or edges with mixed conifers and hardwoods, sunny slopes and roadsides. Likes the sunny side but grows well in the shade and is strong against cold. Its height is 1 ~ 2m, many branches come out, and it is limp on the ground like a long vine, so the whole is like a round bush. Twigs are round and have hairs or cilia. The leaves are 2 ~ 5cm long, triangular broad egg-shaped, pointed at the end, sharp and deep teeth at the edges, and may be divided into several branches. Reddish petiole has fine hairs. Flowers bloom from May to June with yellowish white flowers with a diameter of 4-5mm and run in cones at the end of the branches. Calyx leaves are sharp at the end, 5 petals, 10 stamens, shorter than petals. Fruits are round or egg-shaped, with a lot of fine hairs and ripen in August-September.
대한민국특허 제523443호에는 자생식물군으로부터 오리나무, 고로쇠나무, 국수나무로 구성된 그룹에서 선택된 하나 이상의 추출물을 주성분으로 하여 비만 예방 및/또는 치료용 조성물을 개시하고 있고, 대한민국 공개특허 제2011-0068923호에는 항히스타민제 조성물의 유효성분으로서 국수나무 잎 추출물을 보고하고 있다. Korean Patent No. 523443 discloses a composition for the prevention and / or treatment of obesity, based on one or more extracts selected from the group consisting of alder, cypress, and noodle from the native plant group, and Republic of Korea Patent Publication No. 2011-0068923 The issue reports a Noodle Leaf Extract as an active ingredient of an antihistamine composition.
또한, 미국공개특허 제2009-68291호에는 체외 단백질 분해 효소 억제제를 포함하는 식물 추출물로서 국수나무 추출물을 개시하고 그 활성을 보고하고 있다. In addition, US Patent Publication No. 2009-68291 discloses a Noodle Extract as a plant extract comprising an in vitro protease inhibitor and reports its activity.
이에, 본 발명자들은 국내 자생식물에 대한 생리활성 연구를 통해 국수나무(Stephanandra incisa (Thunb.) Zabel) 추출물이 염증반응을 유도하는 리포폴리싸카라이드(LPS)를 처리한 후 NO 생성저해, 사이클로옥시제나제-2(COX-2) 및 일산화질소합성효소(iNOS) 생성억제의 면역활성에 의한 항노화 효능과 피부에 독성 자극이 없는 안정성을 확인하고, 이를 유효성분으로 함유하는 피부유용성 용도를 제공함으로써, 본 발명을 완성하였다. Accordingly, the present inventors have investigated the physiological activity of domestic native plants, and the extract of N. japonica ( Stephanandra incisa ( Thunb.) Zabel) was treated with lipopolysaccharide (LPS), which induces an inflammatory response, and inhibited NO production. To confirm the anti-aging effect of the immune activity of the generation of Xenase-2 (COX-2) and nitric oxide synthase (iNOS) and the stability without toxic stimulation to the skin, and to provide a skin useful use containing it as an active ingredient By this, the present invention was completed.
본 발명의 목적은 국내 자생식물군 중에서 염증 발병 인자에 대한 생성억제의 면역활성에 의한 항노화 효과를 가지는 국수나무 추출물을 함유하는 조성물을 제공하는 것이다.It is an object of the present invention to provide a composition containing a Noodle extract having an anti-aging effect by the immunosuppressive activity of the production inhibitory factor against inflammatory onset factors among domestic native plant groups.
본 발명의 다른 목적은 상기 국수나무 추출물을 유효성분으로 함유하는 피부 항노화 화장료 조성물을 제공하는 것이다.Another object of the present invention to provide a skin anti-aging cosmetic composition containing the noodle extract as an active ingredient.
본 발명의 또 다른 목적은 상기 국수나무 추출물을 유효성분으로 함유하는 피부외용제 조성물을 제공하는 것이다. Still another object of the present invention is to provide an external preparation composition for skin containing the noodle extract as an active ingredient.
본 발명은 염증 발병 인자에 대한 생성억제의 면역활성에 의한 항노화 효과를 가지는 국수나무(Stephanandra incisa (Thunb.) Zabel) 추출물을 함유하는 조성물을 제공한다.The present invention has an anti-aging effect by the immune activity of the production inhibitory to inflammatory pathogens ( Stephanandra) incisa (Thunb.) Zabel) Provides a composition containing an extract.
더욱 구체적으로 국수나무 추출물은 염증반응을 유도하는 리포폴리싸카라이드(LPS)처리 후, NO 생성저해 및 사이클로옥시제나제-2(COX-2) 및 일산화질소합성효소(iNOS) 생성억제의 면역활성을 가지므로, 항노화 또는 피부유용성 효과를 제공한다. More specifically, after extracting lipopolysaccharide (LPS) to induce an inflammatory response, Noodle extract extracts inhibited NO production and inhibited the production of cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS). Since it has an anti-aging or skin useful effect.
이상의 국수나무 추출물은 국수나무 식물의 꽃, 잎, 줄기, 열매, 종자, 뿌리 및 전초 추출물로 이루어진 군에서 선택되는 어느 하나 또는 2종이상의 혼합부위로부터 추출되며, 바람직하게는 물, 헥산, 메탄올, 에탄올 및 부틸렌글리콜으로 이루어진 군에서 선택되는 단독 또는 그들의 혼합형태의 추출용매에 의해 추출된다. The noodle extract is extracted from any one or two or more mixed sites selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and starch extracts of the Noodle plant, preferably water, hexane, methanol, It is extracted by an extraction solvent alone or in a mixture thereof selected from the group consisting of ethanol and butylene glycol.
이에, 본 발명은 이상의 국수나무 추출물 0.001중량% 이상을 유효성분으로 함유한 피부 항노화 화장료 조성물을 제공한다. Thus, the present invention provides a skin anti-aging cosmetic composition containing more than 0.001% by weight of the noodle extract as an active ingredient.
또한, 본 발명은 이상의 국수나무 추출물 0.001중량% 이상을 유효성분으로 함유한 피부외용제 조성물을 제공한다. The present invention also provides a topical skin composition containing more than 0.001% by weight of noodle extract as an active ingredient.
본 발명의 국수나무 추출물은 노화진행에 따라 생성이 증가되는 염증 발병 인자에 대한 생성억제 활성을 가지며, 구체적으로 염증반응을 유도하는 LPS 처리 후, 면역 반응에 의한 NO 생성저해, 사이클로옥시제나제-2(COX-2) 및 일산화질소합성효소(iNOS) 발현에 대한 억제활성을 보이는 항노화 효과와 더불어 피부에 독성 및 자극이 없어 안정성이 우수함을 확인함으로써, 항염증 및 항노화 용도에 유효한 국수나무 추출물을 함유하는 조성물을 제공할 수 있다. Noodle extract of the present invention has a production inhibitory activity against inflammatory pathogens that increase in production as aging progress, specifically after the LPS treatment to induce an inflammatory response, NO production inhibition by the immune response, cyclooxygenase- Noodle tree effective for anti-inflammatory and anti-aging applications by confirming its excellent stability due to its anti-aging effect showing inhibitory activity against 2 (COX-2) and nitric oxide synthase (iNOS) expression and no toxicity and irritation to skin A composition containing an extract can be provided.
이에, 본 발명은 국수나무 추출물을 유효성분으로 함유하여 피부 항노화 화장료 조성물 및 피부 외용제 조성물을 제공할 수 있다. Thus, the present invention can provide a skin anti-aging cosmetic composition and an external composition for skin by containing the extract of the tree as an active ingredient.
도 1은 본 발명의 국수나무 메탄올 추출물에 따른 세포생존능 측정 결과이고, 1 is a cell viability measurement results according to the methanol extract of noodle tree of the present invention,
도 2는 본 발명의 국수나무 추출물에 따른 LPS-활성화 마우스 대식세포(Raw264.7)에서 NO(Nitrite Oxide) 생성 억제효과를 나타낸 결과이고, 2 is a result showing the inhibitory effect of NO (Nitrite Oxide) production in LPS-activated mouse macrophages (Raw264.7) according to the extract of the Noodle Tree of the present invention,
도 3은 본 발명의 국수나무 추출물에 따른 LPS-활성화 마우스 대식세포(Raw264.7)에서 COX-2와 iNOS 발현에 미치는 저해효과를 나타낸 결과이다. Figure 3 is a result showing the inhibitory effect on the expression of COX-2 and iNOS in LPS-activated mouse macrophages (Raw264.7) according to the extract of the Noodle Tree of the present invention.
이하, 본 발명을 상세히 설명하고자 한다. Hereinafter, the present invention will be described in detail.
본 발명은 염증 발병 인자에 대한 생성억제의 면역활성에 의한 항노화 효과를 가지는 국수나무(Stephanandra incisa (Thunb.) Zabel) 추출물을 함유하는 조성물을 제공한다.The present invention has an anti-aging effect by the immune activity of the production inhibitory to inflammatory pathogens ( Stephanandra) incisa (Thunb.) Zabel) Provides a composition containing an extract.
즉, 본 발명의 실시예에서는 국수나무 추출물에 의해 염증반응을 유도하는 LPS 처리 후, NO 생성저해 및 사이클로옥시제나제-2(COX-2) 및 일산화질소합성효소(iNOS) 생성억제의 면역활성을 구현한다. That is, in the embodiment of the present invention, after the LPS treatment to induce an inflammatory response by the Noodle Extract, the NO activity inhibition and the cyclooxygenase-2 (COX-2) and the nitric oxide synthase (iNOS) production inhibitory activity Implement
본 발명의 국수나무 추출물에 있어서, 국수나무 부위를 모두 포함할 수 있으며, 그 일례로는 국수나무 식물의 꽃, 잎, 줄기, 열매, 종자, 뿌리 및 전초 추출물로 이루어진 군에서 선택되는 어느 하나 또는 2종이상의 혼합부위로부터 추출될 수 있다. 더욱 바람직하게는 본 발명의 실시예에서 국수나무의 잎과 줄기의 혼합형태로부터 추출된 추출물의 경우 염증 발명 인자에 대한 더욱 우수한 면역활성을 구현하나, 이에 한정되지 아니할 것이다. In the noodle extract of the present invention, it may include all the noodle parts, for example, any one selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and starch extracts of the noodle tree plant or It can be extracted from two or more mixed sites. More preferably, in the embodiment of the present invention, the extract extracted from the mixed form of the leaves and stems of the noodle tree implements more excellent immunological activity against the inflammatory invention factor, but is not limited thereto.
상기 국수나무 부위별 추출물에 있어서, 각 추출물은 물, 헥산, 메탄올, 에탄올 및 부틸렌글리콜으로 이루어진 군에서 선택되는 단독 또는 그들의 혼합형태의 추출용매를 이용하여 추출될 수 있으며, 바람직하게는 메탄올 추출물의 경우 우수한 활성을 보인다. 이때, 메탄올 및 알코올을 포함하는 알코올류의 추출용매는 그 순도에 특별한 제한없이 사용될 수 있으나 바람직하게는 80∼95% 메탄올 및 70∼80%의 에탄올을 사용할 수 있다. In the extract according to the noodle parts, each extract may be extracted using an extraction solvent of a single or mixed form selected from the group consisting of water, hexane, methanol, ethanol and butylene glycol, preferably methanol extract In the case of excellent activity. At this time, the extraction solvent of alcohols including methanol and alcohol may be used without particular limitation on the purity, but preferably 80 to 95% methanol and 70 to 80% ethanol.
또한, 본 발명의 국수나무 추출물은 상기의 추출용매를 이용하여 45∼120℃에서 30분∼24시간 동안 가열하여 열수 추출되거나, 5∼40℃에서 30분∼15일간 침지하여 진탕 추출하는 방법에서 선택된 적어도 하나의 추출 방법에 의해 제조될 수 있으며, 추출용매 및 추출방법에 따라 추출물의 수율을 향상시킬 수 있다. In addition, the noodle extract of the present invention is heated to extract hot water by heating at 45 ~ 120 ℃ for 30 minutes to 24 hours using the above extraction solvent, or in a method of extracting shaking by immersion at 5 ~ 40 ℃ for 30 minutes to 15 days It can be prepared by at least one extraction method selected, it is possible to improve the yield of the extract according to the extraction solvent and the extraction method.
이때, 추출용매의 사용량은 각별히 한정되는 것은 아니나, 식물소재 대비 과량 사용되면 추출물이 충분히 추출될 수 없고, 추출 후 추출용매를 제거하고 건조 추출물을 얻는 데 시간과 비용이 많이 들게 된다. 따라서, 열수 추출할 경우 식물 소재를 충분히 포함하고, 추출 효율을 높이기 위해서 가온된 상태로 추출하는 것이 바람직하며, 온도가 너무 낮으면 가온의 이점을 충분히 살리기 어렵고 긴 시간동안 추출해야 하며, 온도가 너무 높으면 유용물질의 분해가 생길 수 있다. At this time, the amount of the extraction solvent is not particularly limited, but when used in excess of the plant material, the extract cannot be extracted sufficiently, it takes a lot of time and money to remove the extraction solvent and obtain a dry extract after extraction. Therefore, in the case of hot water extraction, it is preferable to include a sufficient amount of plant material, and to extract it in a heated state in order to increase the extraction efficiency. If the temperature is too low, it is difficult to fully utilize the benefits of warming and must be extracted for a long time. Higher levels may lead to degradation of useful substances.
또한, 진탕 추출할 경우 상온에서 추출할 수 있으며, 분말 상태의 시료를 충분한 시간 동안, 바람직하게는 3일 정도의 시간 동안 충분한 추출용매에 침지하여 유효성분이 충분히 추출되도록 하며, 추출이 충분하지 못한 경우 반복 공정을 통하거나 추출 시간을 증가시켜 추출량을 늘릴 수 있을 것이다.In addition, when shaking extraction can be extracted at room temperature, the powdered sample is immersed in a sufficient extraction solvent for a sufficient time, preferably for about 3 days to ensure that the effective ingredient is extracted sufficiently, if the extraction is not sufficient The extraction volume may be increased by an iterative process or by increasing the extraction time.
이상의 각 추출용매로부터 추출된 국수나무 추출물은 100㎍/㎖ 이하 농도조건에서 세포독성이 관찰되지 않음으로써[도 1], 피부 적용에 유용하다. Noodle extract extracted from each of the above extraction solvents is not useful for skin application because no cytotoxicity was observed under the concentration condition of 100 μg / ml [ FIG. 1 ].
또한, 본 발명의 각 추출용매로부터 추출된 국수나무 추출물에 대하여 마우스 대식세포(Raw 264.7)에서 NO 생성에 미치는 영향을 관찰한 결과, LPS를 처리한 대조군에 비해, 각 국수나무 추출물 100㎍/㎖ 농도에서 30∼40% 수준으로 NO 생성 억제효과를 확인할 수 있다[도 2]. In addition, as a result of observing the effect on NO production in mouse macrophages (Raw 264.7) with respect to the noodle extract extracted from each extract solvent of the present invention, each noodle extract 100㎍ / ㎖ compared to the control group treated with LPS It is possible to confirm the NO production inhibitory effect at a level of 30 to 40% at the concentration [ FIG. 2 ].
또한, 본 발명의 국수나무 추출물이 LPS-활성화 마우스 대식세포(Raw 264.7)에서 COX-2와 iNOS 발현에 미치는 저해효과를 측정한 결과, 국수나무 추출물은 농도 의존적으로 COX-2와 iNOS 저해 효과를 나타낸다. 특히, 도 3에서 확인되는 바와 같이, 100㎍/㎖ 농도에서 iNOS 발현이 억제된 결과를 확인할 수 있다. In addition, as a result of measuring the inhibitory effect of the noodle extract of the present invention on COX-2 and iNOS expression in LPS-activated mouse macrophages (Raw 264.7), the noodle extract extracts COX-2 and iNOS inhibitory effect in a concentration-dependent manner. Indicates. In particular, as confirmed in Figure 3 , it can be confirmed that the result of the iNOS expression is suppressed at a concentration of 100㎍ / ㎖.
이에, 본 발명의 국수나무 추출물은 염증반응을 유도하는 LPS 처리 후, NO 생성저해 및 COX-2, iNOS 발현을 탁월하게 억제하는 효과를 가지며, 100㎍/㎖ 이하 농도조건에서 피부에 독성 및 자극이 없어 안정성 면에서도 우수하므로, 이를 피부 항노화 화장료 조성물 또는 피부외용제 조성물의 유효성분으로 함유할 수 있다. Thus, the Noodle extract of the present invention has an effect of inhibiting NO production and COX-2, iNOS expression after LPS treatment to induce an inflammatory response, and toxic and irritating to the skin at the concentration of 100 ㎍ / ㎖ or less Since it is excellent in terms of stability, it can be contained as an active ingredient of a skin anti-aging cosmetic composition or a topical skin composition.
이에, 본 발명은 상기 국수나무 추출물을 유효성분으로 함유한 피부 항노화 화장료 조성물을 제공한다. Accordingly, the present invention provides a skin anti-aging cosmetic composition containing the noodle extract as an active ingredient.
또한, 본 발명은 상기 국수나무 추출물을 유효성분으로 함유한 항염증 및 항노화 효능을 가지는 피부외용제 조성물을 제공한다. In addition, the present invention provides an external skin composition having an anti-inflammatory and anti-aging effect containing the noodle extract as an active ingredient.
이때, 본 발명의 피부 항노화 화장료 조성물의 경우는 유연화장수, 영양화장수, 에센스, 영양크림, 마사지 또는 팩 제형에 적용될 수 있고, 피부외용제 조성물은 크림, 젤, 패취, 분무제, 연고제, 경고제, 로션제, 리니멘트(liniments)제, 파스타(pasta)제 또는 카타플라스마(cataplasma)제 제형에 적용될 수 있다. At this time, in the case of the skin anti-aging cosmetic composition of the present invention can be applied to softening longevity, nourishing longevity, essence, nourishing cream, massage or pack formulation, the external skin composition is a cream, gel, patch, spray, ointment, warning, It may be applied to lotion, liniments, pasta or cataplasma formulations.
또한, 상기 조성물의 총 중량에 대하여, 국수나무 추출물은 0.001중량% 이상을 유효성분으로 함유하는 것이 바람직하며, 상기 함량은 염증 발병 인자에 대한 생성억제 활성을 구현하는 최소함량으로 선정된다. 다만, 국수나무 추출물의 안정성과 면역활성을 고려하여 유효함량을 높여 함유할 수 있을 것이나, 피부적용시 변색, 변취 등을 고려하여 바람직하게는 30 중량% 이내 범위로 함유된다.In addition, with respect to the total weight of the composition, noodle extract is preferably contained as 0.001% by weight or more as an active ingredient, the content is selected as the minimum content to implement the production inhibitory activity against the inflammatory disease. However, the effective content may be increased in consideration of stability and immune activity of noodle extract, but is preferably contained within 30% by weight in consideration of discoloration and deodorization when applying skin.
이하, 본 발명을 실시예를 통하여 더욱 상세히 설명하고자 한다. Hereinafter, the present invention will be described in more detail with reference to Examples.
이들 실시예는 오로지 본 발명을 보다 구체적으로 설명하기 위한 것으로서, 본 발명의 요지에 따라 본 발명의 범위가 이들 실시 예에 제한되지 않는다는 것은 이 기술분야에서 통상의 지식을 가진 자에게 있어서 자명할 것이다.These examples are only for illustrating the present invention in more detail, and it will be apparent to those skilled in the art that the scope of the present invention is not limited to these embodiments according to the gist of the present invention. .
<실시예 1> 국수나무 열수 추출물의 제조Example 1 Preparation of Noodle Tree Hydrothermal Extract
국수나무(Stephanandra incisa (Thunb.) Zabel) 잎, 줄기 추출성분과 물을 1:20 중량비로 혼합하여 열수 추출한 후, 380 메쉬로 여과한 후, 0.45㎛ 멤브레인 필터(Supor®-450, 60173, PALL Life Sciences)로 재여과하였다. 이후 여과액을 냉각 콘덴서가 달린 증류장치에서 40℃로 감압 농축한 후 감압증발하여 국수나무 잎, 줄기 추출물 5.8g을 제조하였다.Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) A mixture of leaf, stem extract and water in a 1:20 weight ratio was extracted with hot water, filtered through a 380 mesh, and then re-filtered with a 0.45 μm membrane filter (Supor®-450, 60173, PALL Life Sciences). Filtered. Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare noodle leaves and stem extracts 5.8 g.
<실시예 2> 국수나무 헥산 추출물의 제조 Example 2 Preparation of Noodle Extract Hexane
국수나무(Stephanandra incisa (Thunb.) Zabel) 잎, 줄기 추출성분과 헥산을 1:20 중량비로 혼합하여 3일 동안 상온 침지하였고, 상기 과정을 3회 반복하여 추출한 후, 380 메쉬로 여과하여 0.45㎛ 멤브레인 필터(Supor®-450, 60173, PALL Life Sciences)로 재여과하였다. 이후 여과액을 냉각 콘덴서가 달린 증류장치에서 40℃로 감압 농축한 후 감압증발하여, 국수나무 헥산 추출물6.4g을 제조하였다.Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Leaves, stem extract and hexane was mixed in a 1:20 weight ratio at room temperature immersion for 3 days, the process was extracted three times, and then filtered through a 380 mesh 0.45㎛ membrane filter (Supor® -450, 60173, PALL Life Sciences). Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare 6.4 g of noodle extract.
<실시예 3> 국수나무 메탄올 추출물의 제조Example 3 Preparation of Noodle Tree Methanol Extract
국수나무( Stephanandra incisa (Thunb.) Zabel) 잎, 줄기 추출성분과 메탄올을 1:20 중량비로 혼합하여 3일 동안 상온 침지하였고, 상기 과정을 3회 반복하여 추출한 후, 380 메쉬로 여과하여 0.45㎛ 멤브레인 필터(Supor®-450, 60173, PALL Life Sciences)로 재여과하였다. 이후 여과액을 냉각 콘덴서가 달린 증류장치에서 40℃로 감압 농축한 후 감압증발하여, 국수나무 메탄올 추출물6.8g을 제조하였다.Noodles ( Stephanandra incisa (Thunb.) Zabel) Leaves, stem extracts and methanol were mixed in a 1:20 weight ratio at room temperature for 3 days, and the process was repeated three times, followed by extraction with a 380 mesh to filter 0.45㎛ membrane filter (Supor® -450, 60173, PALL Life Sciences). Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare ethanol methanol extract 6.8 g.
<실시예 4> 국수나무 에탄올 추출물의 제조Example 4 Preparation of Noodle Ethanol Extract
국수나무(Stephanandra incisa (Thunb.) Zabel) 잎, 줄기 추출성분과 에탄올을 1:20 중량비로 혼합하여 3일 동안 상온 침지하였고, 상기 과정을 3회 반복하여 추출한 후, 380 메쉬로 여과하여 0.45㎛ 멤브레인 필터(Supor®-450, 60173, PALL Life Sciences)로 재여과하였다. 이후 여과액을 냉각 콘덴서가 달린 증류장치에서 40℃로 감압 농축한 후 감압증발하여, 국수나무 에탄올 추출물5.1g을 제조하였다.Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Leaves, stem extracts and ethanol were mixed at 1:20 weight ratio to immerse at room temperature for 3 days, extracted three times, and then filtered through a 380 mesh 0.45 ㎛ membrane filter (Supor®). -450, 60173, PALL Life Sciences). Thereafter, the filtrate was concentrated under reduced pressure at 40 ° C. in a distillation apparatus equipped with a cooling condenser and evaporated under reduced pressure to prepare 5.1 g of noodle ethanol extract.
<실시예 5> 국수나무 부틸렌글리콜 추출물의 제조Example 5 Preparation of Noodle Butyl Glycol Extract
국수나무(Stephanandra incisa (Thunb.) Zabel) 잎, 줄기 추출성분과 부틸렌글리콜을 1:20 중량비로 혼합하여 3일 동안 상온 침지하였고, 상기 과정을 3회 반복하여 추출한 후, 380 메쉬로 여과하여 0.45㎛ 멤브레인 필터로 재여과하여, 국수나무 부틸렌글리콜 추출물을 제조하였다.Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) leaves, stem extract ingredients and butylene glycol were mixed in a 1:20 weight ratio, immersed at room temperature for 3 days, the process was extracted three times, and then filtered through a 380 mesh to 0.45 ㎛ membrane filter Refiltration was performed to prepare a noodle butylene glycol extract.
<실시예 6> 국수나무 열매 추출물의 제조Example 6 Preparation of Noodle Fruit Extract
국수나무(Stephanandra incisa (Thunb.) Zabel) 열매 성분을 사용하는 것을 제외하고는, 상기 실시예 3과 동일하게 수행하여, 국수나무 열매 메탄올 추출물 5.7g을 제조하였다. Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Except for using the fruit component, was carried out in the same manner as in Example 3, to prepare a 5.7 g of Noodle fruit methanol extract.
<실시예 7> 국수나무 뿌리 추출물의 제조Example 7 Preparation of Noodle Root Extract
국수나무(Stephanandra incisa (Thunb.) Zabel) 뿌리 성분을 사용하는 것을 제외하고는, 상기 실시예 3과 동일하게 수행하여, 국수나무 뿌리 메탄올 추출물 4.2g을 제조하였다. Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Except for using the root component, it was carried out in the same manner as in Example 3, to prepare 4.2g of Noodle root methanol extract.
<실시예 8> 국수나무 전초 추출물의 제조Example 8 Preparation of Noodle Tree Outpost Extract
국수나무(Stephanandra incisa (Thunb.) Zabel) 전초 성분을 사용하는 것을 제외하고는, 상기 실시예 3과 동일하게 수행하여, 국수나무 전초 메탄올 추출물 6.3g을 제조하였다. Noodle Tree ( Stephanandra incisa (Thunb.) Zabel) Except for using the starch component, it was carried out in the same manner as in Example 3, to prepare 6.3g of methanol extract Noodle outpost.
<실험예 1> MTT 세포생존능 측정 Experimental Example 1 Measurement of MTT Cell Viability
상기 실시예 1∼5에서 제조된 국수나무 추출물들에 대한 세포독성을 확인하기 위하여, 마우스 대식세포주인 Raw264.7 셀을 5×10⁴cells/㎖ 농도로 하여 96-웰 플레이트에 100㎕씩 분주하여 37℃, CO2 인큐베이터에서 6시간 동안 배양하였다. 이때 배지는 소혈청 10%와 항생제 1%가 포함된 DMEM/high glucose(Dulbeco's Modified Eagle Medium, Hyclone)를 이용하였다.In order to confirm the cytotoxicity of the Noodle extracts prepared in Examples 1 to 5, 100 μl of a mouse macrophage line, Raw264.7 cells, was injected into a 96-well plate at a concentration of 5 × 10 μs cells / ml and 37 C, incubated for 6 hours in a CO 2 incubator. At this time, DMEM / high glucose (Dulbeco's Modified Eagle Medium, Hyclone) containing 10% bovine serum and 1% antibiotic was used.
상기 중에서 실시예 3에서 제조된 국수나무 메탄올 추출물을 농도 100, 75, 50, 25, 10, 5, 1, 0.5, 0.1㎍/㎖로 96-웰 플레이트에 시료 처리하여 37℃, CO2 인큐베이터에서 24시간 동안 배양하였다. The noodle methanol extract prepared in Example 3 above was sampled in a 96-well plate at a concentration of 100, 75, 50, 25, 10, 5, 1, 0.5, 0.1 µg / ml in a 37 ° C., CO 2 incubator. Incubated for 24 hours.
24시간 후 MTT 용액을 웰 당 10㎕씩 처리하여 37℃, CO2 인큐베이터에 배양 3시간 후에 MTT를 제거하고 DMSO를 웰당 100㎕씩 주입하여 20분 흔들어(Shaking) 570nm/ 655nm 흡광도를 측정하였다. After 24 hours, 10 μl of MTT solution was treated per well, and after 3 hours of incubation at 37 ° C. in a CO 2 incubator, MTT was removed, and 100 μl of DMSO was injected per well for 20 minutes, shaking (570 nm / 655 nm) of absorbance was measured.
상기 실험결과를 도 1에 도시하였으며, 상기 실시예 3에서 제조된 국수나무 메탄올 추출물 100㎍/㎖이하의 농도조건에서 세포독성을 나타내지 않음을 확인하였다. The experimental results are shown in FIG. 1, and it was confirmed that no cytotoxicity was observed under the concentration condition of 100 μg / ml or less of the methanol extract of Noodle tree prepared in Example 3.
<실험예 2> NO 생성평가 측정Experimental Example 2 Measurement of NO Production Evaluation
상기 실시예 1∼8에서 제조된 국수나무 추출물들에 대한 세포독성을 확인하기 위하여, 마우스 대식세포주인 Raw264.7 세포를 5×10 cells/㎖ 농도로 96-웰 플레이트에100㎕씩 분주하여 37℃, CO2 인큐베이터에서 24시간 동안 배양하였다. 이때, 배지는 소혈청 10%와 항생제 1%가 포함된 DMEM/high glucose(Dulbeco's Modified Eagle Medium, Hyclone)를 이용하였다. In order to confirm the cytotoxicity of the Noodle extracts prepared in Examples 1 to 8, 100 μl of mouse macrophage Raw264.7 cells were injected into 96-well plates at a concentration of 5 × 10 cells / ml and 37 μl. C, incubated for 24 hours in a CO 2 incubator. In this case, DMEM / high glucose (Dulbeco's Modified Eagle Medium, Hyclone) containing 10% bovine serum and 1% antibiotic was used.
상기에서 제조된 국수나무 메탄올 추출물을 100, 50, 25㎍/㎖ 농도로 96-웰 플레이트에 시료 처리하여 DMEM(Dulbecco’s modified Eagle’s medium) 90㎕, 시료 10㎕) 37℃, CO2 인큐베이터에서 30분 동안 반응시킨 후, LPS (Lipopolysaccharide) 1㎍/㎖씩 처리하여 37℃, CO2 인큐베이터에서 24시간 동안 배양하였다. Methanol extract prepared above was sampled in a 96-well plate at a concentration of 100, 50 or 25 µg / ml, and 90 µl of DMEM (Dulbecco's modified Eagle's medium) and 10 µl of sample at 37 ° C. in a CO 2 incubator. After the reaction, LPS (Lipopolysaccharide) was treated with 1 ㎍ / ㎖ and incubated for 24 hours at 37 ℃, CO 2 incubator.
생성된 NO(Nitrite Oxide) 양은 그리스(Griess) 시약(NED solution, sulfanilamide solution)을 이용하여 세포배양액 중에 존재하는 NO₂형태로 측정하였다. 세포배양 상등액 50㎕와 그리스 시약 (NED solution, sulfanilamide solution) 각각 50㎕씩 처리하여 10분간 실온에서 빛을 차단하여 반응시킨 후, 540nm/655nm 흡광도를 측정하고, 그 실험결과를 하기 표 1 및 표 2에 기재하였다. The amount of NO (Nitrite Oxide) produced was measured in the form of NO₂ present in the cell culture solution using a Greries reagent (NED solution, sulfanilamide solution). Cell culture supernatant 50㎕ and Greece reagent (NED solution, sulfanilamide solution), respectively after the treatment by 50㎕ reacted by blocking the light for 10 minutes at room temperature, and measuring the 540nm / 655nm absorbance, to the results in Table 1 and Table 2 is described.
Figure PCTKR2015007654-appb-I000001
Figure PCTKR2015007654-appb-I000001
Figure PCTKR2015007654-appb-I000002
Figure PCTKR2015007654-appb-I000002
상기 표 1 및 표 2에서 가장 우수한 NO(Nitrite Oxide)의 생성 억제효과를 보인 실시예 3의 국수나무 추출물에 대하여, 도 2는 본 발명의 실시예 3에 국수나무 추출물에 따른 LPS-활성화 마우스 대식세포(Raw264.7)에서 NO(Nitrite Oxide) 생성 억제효과를 나타낸 도시하였다. For the noodle extract of Example 3 showing the best inhibitory effect of NO (Nitrite Oxide) production in Table 1 and Table 2, Figure 2 is LPS-activated mouse vs. Noodle extract in Example 3 of the present invention NO (Nitrite Oxide) production inhibitory effect was shown in phagocytes (Raw264.7).
그 결과, 100㎍/㎖ 이하의 조건에서 염증 유발물질인 LPS에 의하여 유도된 NO(Nitrite Oxide)의 생성 억제효과를 확인하였으며, 특히, 100㎍/㎖ 농도에서 가장 높은 NO(Nitrite Oxide)의 생성 억제효과를 확인하였다.As a result, the effect of inhibiting the production of NO (Nitrite Oxide) induced by LPS, an inflammation-inducing substance under conditions of 100 µg / ml or less, in particular, the highest production of NO (Nitrite Oxide) at 100 µg / ml The inhibitory effect was confirmed.
<실험예 3> iNOS 및 COX-2 발현 측정Experimental Example 3 Measurement of iNOS and COX-2 Expression
RAW 264.7 세포 (5×105 cells/㎖)를 18시간 동안 전 배양하고, 시험물질(실시예 3의 국수나무 메탄올 추출물 100, 50, 25㎍/㎖)과 LPS(1㎍/㎖)를 동시 처리하여 24시간 배양하였다. RAW 264.7 cells (5 × 10 5 cells / ml) were pre-incubated for 18 hours, and the test substance (100, 50, 25 µg / ml of methanol extract of Example 3) and LPS (1 µg / ml) simultaneously Treated and incubated for 24 hours.
24시간 배양 후 세포를 수거하고 PBS로 두 번 세척하였다. 이후 세포를 버퍼용액[Pro-PREPTM solution]에 용해하고 원심분리시킨 후 상등액을 수거하여 사용 전까지 70℃에서 보관하였다. 단백질의 농도는 브래드포드(Bradford) 방법으로 정량하였다. 세포 용해 상등액을 SDS-폴리아크릴아미드 겔 상에서 전기영동하고 PVDF 막(BIO-RAD, HC, USA)에 전이시킨 후 블락킹(blocking) 용액(5% nonfat dried milk)으로 블락킹하였다. After incubation for 24 hours, cells were harvested and washed twice with PBS. Cells were then dissolved in buffer [Pro-PREP ™ solution], centrifuged and the supernatant collected and stored at 70 ° C. until use. The concentration of protein was quantified by the Bradford method. The cell lysis supernatants were electrophoresed on SDS-polyacrylamide gels and transferred to PVDF membranes (BIO-RAD, HC, USA) and then blocked with blocking solution (5% nonfat dried milk).
anti-iNOS 항체 및 anti-COX-2 항체를 이용하여 밤새(overnight) 방치한 후 2차 항체를 넣고 반응시켰다. 그 다음 ECL 웨스턴 블롯팅 검출 키트로 검출하고 액틴(actin)과 비교하여 장비(chemi-Doc)를 이용하여 단백질의 발현 정도를 측정하였다. After leaving overnight with anti-iNOS antibody and anti-COX-2 antibody, the secondary antibody was added and reacted. It was then detected with an ECL western blotting detection kit and the expression level of the protein was measured using a device (chemi-Doc) compared to actin.
그 실험결과를 도 3에 도시하였으며, 100㎍/㎖ 농도에서 iNOS 발현이 억제된 결과를 확인하였다. The experimental results are shown in FIG. 3, and the results showed that iNOS expression was suppressed at a concentration of 100 μg / ml.
<처방예 1> 유연화장수 제조<Prescription 1> Manufacturing flexible cosmetic water
상기 실시예의 3에서 제조된 국수나무 메탄올 추출물을 유효성분으로 함유한 화장료 중 유연화장수에 대한 제형예는 하기 표 3과 같다.Formulation examples for the flexible cosmetics in the cosmetics containing the methanol extract of the noodle tree prepared in Example 3 as an active ingredient is shown in Table 3 below.
더욱 구체적으로는, 소듐히아루로네이트는 정제수에 프로펠러믹서 3000rpm으로 분산하여 1% 용액으로 준비하였다. 수상 용해조에 원료 1∼8을 프로펠러믹서 500rpm으로 균일화하고 75℃에서 가온하여 완전 용해시킨 후, 실온으로 냉각하였다. 별도 용해조에 원료 9∼11을 완전 용해시켜 상기 수상 용해조에, 투입한 후 교반 혼합하였다. 여기에, 실시예의 3에서 제조된 국수나무 메탄올 추출물을 투입하고 충분히 교반 혼합하여, 유연화장수를 제조하였다.More specifically, sodium hyaluronate was prepared as a 1% solution by dispersing at 3000 rpm propeller mixer in purified water. The raw materials 1 to 8 were homogenized at 500 rpm in a propeller mixer in a water phase dissolving tank, warmed at 75 ° C. to completely dissolve, and then cooled to room temperature. The raw materials 9-11 were completely melt | dissolved in the separate dissolution tank, and it put into the said water phase dissolution tank, and stirred and mixed. Into this, noodle methanol extract prepared in Example 3 was added and sufficiently stirred and mixed to prepare a flexible cosmetic water.
Figure PCTKR2015007654-appb-I000003
Figure PCTKR2015007654-appb-I000003
<처방예 2> 영양화장수 제조<Prescription 2> Preparation of nutritional cosmetic water
상기 실시예의 3에서 제조된 국수나무 메탄올 추출물을 유효성분으로 함유한 화장료 중 영양화장수에 대한 제형예는 하기 표 4와 같다.Formulation example for the nutrient cosmetic water in the cosmetics containing the methanol extract of noodle tree prepared in Example 3 as an active ingredient is shown in Table 4 .
카보머는 정제수에 프로펠러믹서 4000rpm으로 분산하여 2% 용액으로 준비하였다. 수상용해조에 원료 1∼6을 투입하여 호모 2000rpm으로 교반하여 분산 후, 75℃까지 가온하였다. 유상 용해조에 원료 7∼13을 투입하여 75℃까지 가온 용해하였다. 수상 용해조에 용해된 유상을 투입하여 유화(3000rpm/5분)시킨 후, 실온으로 냉각하였다. 여기에, 실시예의 3에서 제조된 국수나무 메탄올 추출물을 투입하고 충분히 교반 혼합하여, 영양화장수를 제조하였다.Carbomer was prepared in 2% solution by dispersing with propeller mixer 4000rpm in purified water. The raw materials 1-6 were thrown into the water melting tank, and it stirred at 2000 rpm and disperse | distributed, and heated to 75 degreeC. The raw materials 7-13 were put into the oil phase dissolution tank, and it melt | dissolved by heating to 75 degreeC. The oil phase dissolved in the aqueous phase dissolution tank was introduced and emulsified (3000 rpm / 5 minutes), followed by cooling to room temperature. Into this, noodle methanol extract prepared in Example 3 was added and sufficiently stirred and mixed to prepare nutritious longevity.
Figure PCTKR2015007654-appb-I000004
Figure PCTKR2015007654-appb-I000004
<처방예 3> 에센스 제조<Prescription Example 3> Essence Preparation
상기 실시예의 3에서 제조된 국수나무 메탄올 추출물을 유효성분으로 함유한 화장료 중 에센스에 대한 제형예는 하기 표 5와 같다.Formulation example for the essence of the cosmetics containing the methanol extract of noodle tree prepared in Example 3 as an active ingredient is shown in Table 5 below.
소듐히아루로네이트, 히드록시에틸셀룰로이즈는 각각 정제수에 프로펠러믹서 2000rpm으로 분산하여 1% 용액으로 준비하였다. 카보머는 정제수에 프로펠러믹서 4000rpm으로 분산하여 2% 용액으로 준비하였다. 수상 용해조에 원료 1∼12를 투입하여, 호모 2000rpm으로 교반하여 분산한 후, 75℃까지 가온하였다. 상기 가온된 수상을 실온으로 냉각하였다. 별도의 용해조에 원료 13∼15를 완전 용해시킨 후, 상기 수상 용해조에 투입하여 교반 혼합하였다. Sodium hyaluronate and hydroxyethyl cellulose were each dispersed in purified water at 2000 rpm with a propeller mixer to prepare a 1% solution. Carbomer was prepared in 2% solution by dispersing with propeller mixer 4000rpm in purified water. The raw materials 1-12 were thrown into the aqueous phase dissolution tank, and it stirred at 2000 rpm and disperse | distributed, and heated to 75 degreeC. The warm water phase was cooled to room temperature. After completely dissolving the raw materials 13-15 in another dissolution tank, the solution was added to the aqueous phase dissolution tank and stirred and mixed.
여기에, 여기에, 실시예의 3에서 제조된 국수나무 메탄올 추출물을 투입하고 충분히 교반 혼합하여, 에센스를 제조하였다. Here, the noodle methanol extract prepared in Example 3 was added thereto, followed by sufficiently stirring and mixing to prepare an essence.
Figure PCTKR2015007654-appb-I000005
Figure PCTKR2015007654-appb-I000005
<처방예 4> 영양크림 제조<Prescription 4> Preparation of nutrition cream
상기 실시예의 3에서 제조된 국수나무 메탄올 추출물을 유효성분으로 함유한 화장료 중 영양크림에 대한 제형예는 하기 표 6과 같다.Formulation example for the nourishing cream in the cosmetic composition containing the methanol extract of noodle tree prepared in Example 3 as an active ingredient is shown in Table 6 .
수상용해조에 원료 1∼8을 투입하여 호모 2000rpm으로 교반하여 분산한 후, 75℃까지 가온하였다. 유상 용해조에 원료 9∼16을 투입하여 80℃까지 가온 용해시켰다. 수상용해조에 용해된 유상을 투입하여 유화(3000rpm/10분)시킨 후, 실온으로 냉각하였다. 여기에, 실시예의 3에서 제조된 국수나무 메탄올 추출물을 투입하고 충분히 교반 혼합하여, 영양크림을 제조하였다. The raw materials 1-8 were thrown into the water melting tank, it stirred at 2000 rpm, and it disperse | distributed, and it heated up to 75 degreeC. Raw materials 9-16 were thrown into the oil phase dissolution tank, and it heated and melt | dissolved to 80 degreeC. The oil phase dissolved in the aqueous phase bath was added to emulsify (3000 rpm / 10 minutes), and then cooled to room temperature. Into this, noodle methanol extract prepared in Example 3 was added and sufficiently stirred and mixed to prepare a nourishing cream.
Figure PCTKR2015007654-appb-I000006
Figure PCTKR2015007654-appb-I000006
상기에서 살펴본 바와 같이, 본 발명은 국내 자생식물의 생리활성에 대한 연구를 통해 국수나무(Stephanandra incisa (Thunb.) Zabel) 추출물이 염증 발병 인자에 대한 생성억제의 면역활성에 의한 항노화 효과를 확인함으로써, 항염증 및 항노화 용도에 유효한 국수나무 추출물을 함유하는 조성물을 제공하였다. As described above, the present invention through the study on the physiological activity of domestic native plants ( Stephanandra) By incisa ( Thunb.) Zabel extract confirmed the anti-aging effect by the immunosuppressive activity of the production inhibitory to inflammatory onset factors, to provide a composition containing the Noodle extract effective for anti-inflammatory and anti-aging applications.
이에, 본 발명은 국수나무 추출물이 피부에 독성 및 자극이 없어 그 안정성을 확인함으로써, 이를 유효성분으로 함유한 피부 항노화 화장료 조성물 및 피부외용제 조성물을 제공하였다.Accordingly, the present invention provides a skin anti-aging cosmetic composition and a topical skin composition containing the extract as an active ingredient by checking the stability of the noodle extract does not have toxicity and irritation to the skin.
이상에서 본 발명은 기재된 구체예에 대해서만 상세히 설명되었지만 본 발명의 범위 내에서 다양한 변형 및 수정이 가능함은 당업자에게 있어서 명백한 것이며, 이러한 변형 및 수정이 첨부된 특허청구범위에 속함은 당연한 것이다.While the invention has been described in detail only with respect to the described embodiments, it will be apparent to those skilled in the art that various modifications and variations are possible within the scope of the invention, and such modifications and variations belong to the appended claims.

Claims (6)

  1. 염증 발병 인자에 대한 생성억제의 면역활성에 의한 항노화 효과를 가지는 국수나무(Stephanandra incisa (Thunb.) Zabel) 추출물을 함유하는 조성물. Stephanandra incisa with Anti-Aging Effects by Immune Activity of Inflammatory Pathogens (Thunb.) Zabel) A composition containing an extract.
  2. 제1항에 있어서, 상기 국수나무 추출물이 염증반응을 유도하는 리포폴리싸카라이드(LPS)처리 후, NO 생성저해 및 사이클로옥시제나제-2(COX-2) 및 일산화질소합성효소(iNOS) 생성억제의 면역활성을 가지는 것을 특징으로 하는 조성물.The method according to claim 1, wherein the Noodle extract after the lipopolysaccharide (LPS) treatment to induce an inflammatory response, NO production and cyclooxygenase-2 (COX-2) and nitric oxide synthase (iNOS) production A composition, characterized in that it has an immunosuppressive activity.
  3. 제1항에 있어서, 상기 국수나무 추출물이 국수나무 식물의 꽃, 잎, 줄기, 열매, 종자, 뿌리 및 전초 추출물로 이루어진 군에서 선택되는 어느 하나 또는 2종이상의 혼합부위로부터 추출된 것을 특징으로 하는 조성물.The method according to claim 1, wherein the noodle extract is characterized in that extracted from any one or two or more selected from the group consisting of flowers, leaves, stems, fruits, seeds, roots and starch extracts of the Noodle plant Composition.
  4. 제1항에 있어서, 상기 국수나무 추출물이 물, 헥산, 메탄올, 에탄올 및 부틸렌글리콜으로 이루어진 군에서 선택되는 단독 또는 그들의 혼합형태의 추출용매에 의해 추출된 것을 특징으로 하는 조성물.The composition according to claim 1, wherein the noodle extract is extracted by an extraction solvent in a single or mixed form thereof selected from the group consisting of water, hexane, methanol, ethanol and butylene glycol.
  5. 제1항 내지 제4항 중 어느 한 항의 국수나무 추출물 0.001중량% 이상을 유효성분으로 함유한 항노화 효능을 가지는 화장료 조성물.Cosmetic composition having an anti-aging effect containing at least 0.001% by weight of the noodle extract of any one of claims 1 to 4 as an active ingredient.
  6. 제1항 내지 제4항 중 어느 한 항의 국수나무 추출물 0.001중량% 이상을 유효성분으로 함유한 항염증 및 항노화 효능을 가지는 피부외용제 조성물.The topical skin composition having anti-inflammatory and anti-aging efficacy, containing at least 0.001% by weight of the noodle extract of claim 1 as an active ingredient.
PCT/KR2015/007654 2015-07-22 2015-07-23 Composition containing stephanandra incisa (thunb.) zabel extracts having anti-aging efficacy and use containing same as active ingredient WO2017014340A1 (en)

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