WO2016202155A2 - 一种西兰花孵育粉、压榨汁及其制备方法和应用 - Google Patents

一种西兰花孵育粉、压榨汁及其制备方法和应用 Download PDF

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WO2016202155A2
WO2016202155A2 PCT/CN2016/083432 CN2016083432W WO2016202155A2 WO 2016202155 A2 WO2016202155 A2 WO 2016202155A2 CN 2016083432 W CN2016083432 W CN 2016083432W WO 2016202155 A2 WO2016202155 A2 WO 2016202155A2
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broccoli
incubation
cancer
powder
juice
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PCT/CN2016/083432
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French (fr)
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WO2016202155A3 (zh
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王继栋
张灵坚
张辉
王永东
郑晓鹤
齐欢
唐海玲
叶利娇
白骅
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浙江海正药业股份有限公司
台州市天莱生物科技有限公司
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Publication of WO2016202155A2 publication Critical patent/WO2016202155A2/zh
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Definitions

  • the invention belongs to the field of bioengineering and relates to the treatment and application of broccoli.
  • Broccoli oleracea var.italia is a variant of Brassica oleracea, which is native to Italy and is rich in bioactive substances and nutrients. It is known as the “vegetable crown”.
  • the nutrients in broccoli are not only high in content, but also very comprehensive, mainly including protein, carbohydrates, fats, minerals, vitamin C and carotene.
  • the content of vitamin C is higher than that of the cauliflower belonging to the cruciferous family, about 20%, which is 2-3 times that of cabbage, 5-6 times that of tomato; the content of carotene is more than 30 times of flower; the content of folic acid is 2 of cauliflower. Double; the protein content is 3 times that of cauliflower and 4 times that of tomato.
  • the mineral composition of broccoli is more comprehensive than other vegetables, calcium, phosphorus, iron, potassium, zinc, manganese and other content are very rich, much higher than the cauliflower of the same family.
  • broccoli is a high-fiber vegetable, which can effectively reduce the absorption of glucose in the stomach, thereby lowering blood sugar, and effectively controlling the condition of diabetic patients.
  • the broccoli powder sold on the market is mainly a broccoli powder obtained by directly lyophilizing and pulverizing broccoli, and the vegetable powder has poor dispersibility and solubility in water.
  • the direct lyophilization treatment has not been able to enrich the various nutrients in broccoli, and And lyophilization will inactivate the myrosinase, so that the glucosinolate cannot be converted into an active ingredient such as sulforaphane.
  • the patent application CN201210258522.2 discloses a method for preparing broccoli vegetable powder by spray drying, which comprises the following steps: raw material pretreatment, blanching color protection, drainage, beating, homogenization and spray drying.
  • This processing method improves the dispersibility and solubility of broccoli vegetable powder, and is beneficial for addition to other foods without causing a rough mouth.
  • the broccoli vegetable powder obtained by the method does not have the effect of enriching the nutrient composition of the broccoli, and there is no catalytic process of the black mustard enzyme, and it is difficult to form an active ingredient such as sulforaphane which is beneficial to the human body.
  • the patent application CN201310281689.5 discloses a method for extracting and preparing broccoli concentrated powder, which is carried out according to the following steps: (1) Washing broccoli, removing leaves and other debris, chopping; (2) adding A large amount of ethanol is extracted; (3) filtered, and the extract is collected; (4) vacuum dried at a low temperature; (5) pulverized; (6) packaged and stored.
  • the broccoli extract collected by this method can completely extract the active ingredients from broccoli, and preserve the easily oxidized components such as vitamin C for a long time.
  • this method concentrates the nutrients in broccoli, the method does not include the black mustard enzyme catalyzed process, so the amount of sulforaphane and diterpene methane produced is small; and a large amount is used in the extraction process.
  • ethanol not only greatly increases its cost but also increases the risk of production.
  • the extracted waste residue is difficult to reuse and will pollute the ecological environment.
  • Patent application CN201310126916.7 discloses a broccoli scrap extract and a preparation method thereof, the method comprising: (1) ultrasonically assisting enzymatic hydrolysis after broccoli beating; (2) radish sulphur in the obtained broccoli slurry Extraction with flavonoids.
  • the invention is characterized in that the composite enzyme preparation, the ultrasonic assisted extraction and the microwave assisted extraction are applied to the extraction of the active ingredients in the broccoli scrap, which greatly increases the added value of the broccoli scrap.
  • the introduction of exogenous enzymes in this method has a potential impact on the active nutrients of broccoli.
  • the method also uses a large amount of ethanol, which increases the cost and is not conducive to the comprehensive utilization of waste residue.
  • the present invention provides a method of preparing broccoli incubation powder.
  • the method firstly concentrates the nutrients in broccoli, and then uses broccoli's own enzyme system to convert glucosinolate into anti-tumor components such as sulforaphane and diterpene methane. There is almost no waste discharge in the entire process, reaching green production standards.
  • One aspect of the present invention provides a method for preparing a broccoli incubation powder, comprising:
  • the incubation time of the incubation in the step (3) is 10 to 240 minutes, preferably 20 to 120 minutes, more preferably 30 to 60 minutes.
  • the incubation temperature of the incubation in the step (3) is from 10 to 60 ° C, preferably from 20 to 40 ° C, more preferably from 20 to 30 ° C.
  • step (5) comprises freeze drying or spray drying.
  • Another aspect of the present invention provides a method of preparing broccoli pressed juice, the method comprising:
  • the incubation time of the incubation in the step (3) is 10 to 240 minutes, preferably 20 to 120 minutes, more preferably 30 to 60 minutes.
  • the incubation temperature of the incubation in the step (3) is from 10 to 60 ° C, preferably from 20 to 40 ° C, more preferably from 20 to 30 ° C.
  • a third aspect of the invention relates to a broccoli incubation powder or broccoli pressed juice prepared according to the method of the invention.
  • a fourth aspect of the invention relates to a composition
  • a composition comprising the broccoli incubation powder or the broccoli juice of the invention, the composition being a food, a health supplement or a medicine for lowering blood fat, lowering blood pressure, preventing cancer, Reduce blood sugar and / or improve immunity.
  • a fifth aspect of the invention relates to the broccoli broth or broccoli squeezing juice for use in preparing foods, health products and medicines having the effects of lowering blood fat, lowering blood pressure, preventing cancer, lowering blood sugar and/or improving immunity Use, wherein the cancer in the anti-cancer effect comprises: lung cancer, prostate cancer, colon cancer, breast cancer.
  • a sixth aspect of the invention relates to the use of the broccoli incubation powder or broccoli juice for lowering blood fat, lowering blood pressure, preventing cancer, lowering blood sugar and/or improving immunity; preferably, said anticancer Cancer includes: lung cancer, prostate cancer, colon cancer or breast cancer.
  • Broccoli contains myrosinase, which can hydrolyze glucosinolates from broccoli to form sulforaphane during incubation; in addition, other nutrients such as crude polysaccharides and quinces in broccoli can be incubated by incubation. Methane and vitamin C are extracted more fully.
  • the method of the invention is simple and easy to operate, and has strong operability.
  • the obtained broccoli incubation powder has high nutritional value and good dispersibility and water solubility, and can be used in processing and production of food, health care products and medicines.
  • the reagents and instruments used are all commonly used reagents and instruments in the art, and can be purchased from chemical or biological products/preparation companies; the methods used in the following examples are all conventional methods in the art. Those skilled in the art can undoubtedly know the operation process of these experiments and obtain corresponding results according to the prior art or the operation manual provided by the manufacturer.
  • Protein content National food safety standard for foods (GB 5009.5-2010).
  • Vitamin C content (potentiometric titration: redox indicator electrode): take about 1.0g of this product, accurately weighed, placed in a beaker, add 50mL of fresh boiling cold water and 5mL of dilute acetic acid to dissolve, after 30min ultrasonication, titration; Titration was carried out with an iodine titration solution (0.05 mol/L), and the potential indicating method indicated the end point. Each 1 mL of iodine titration solution (0.05 mol/L) corresponds to 8.806 mg of C 6 H 8 O 6 .
  • Vitamin C (%) 0.4 0.3 0.3 0.3 0.3 0.2 Radish Sulfur (%) 0.022 0.035 0.039 0.051 0.055 0.058
  • the whole broccoli was divided into three parts: flower ball, stem and leaf, and another whole broccoli was taken, and then crushed with a pulverizer. Weigh the same amount of whole broccoli, smashed stalks, stalks and leaves and transfer to a beaker, then incubate in a 20 ° C water bath for 60 minutes, then filter with gauze (300 mesh) ( That is, the filtrate is obtained by pressing, and the filtrate is dried in a freeze dryer to obtain a broccoli incubation powder. The nutrients in the obtained product were measured, and the results are shown in Table 3.
  • Example 3 After the stalks and leaves of the stalks and stalks of Example 3 were incubated, the filter residue obtained by filtration was dried (Shanghai Bo Xun Industrial Co., Ltd. Medical Equipment Factory, electric thermostatic blast drying oven GZX-9140MBE type) to obtain broccoli dried stem and leaf powder.
  • the crude protein content was 22% (the method for determination of crude protein in feed, GB/T 6432-1994), which has the use value and avoids material waste.

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  • Medicines Containing Plant Substances (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Preparation Of Fruits And Vegetables (AREA)

Abstract

本发明属于生物工程领域，公开了一种西兰花孵育粉、压榨汁及其制备方法和应用，所述西兰花孵育粉的制备方法包括：将西兰花的茎、叶和花球三种器官中的至少一种器官粉碎、孵育，然后通过压榨得到压榨汁，压榨汁直接经过冷冻干燥或喷雾干燥得到西兰花孵育粉。本发明的制备方法利用西兰花内源酶系统将自身的硫代葡萄糖苷水解为具有抗癌作用的萝卜硫素，同时也浓缩了西兰花的其他各种营养保健成分，而且工艺简单，可操作性强，得到的孵育粉水分散性好，可用于食品、保健品及药品等领域。

Description

一种西兰花孵育粉、压榨汁及其制备方法和应用 技术领域
本发明属于生物工程领域，涉及西兰花的处理和应用。
背景技术
西兰花(Brassica oleracea var.italia)是由十字花科芸苔属甘蓝变种而成，原产于意大利，含有丰富的生物活性物质和营养物质，被誉为“蔬菜皇冠”。西兰花中的营养成分不仅含量高，而且十分全面，主要包括蛋白质、碳水化合物、脂肪、矿物质、维生素C和胡萝卜素等。其中维生素C含量高于同属于十字花科的菜花20％左右，是洋白菜的2-3倍，是番茄的5-6倍；胡萝卜素的含量是花的30多倍；叶酸含量是菜花的2倍；蛋白质含量是菜花的3倍，是番茄的4倍。此外，西兰花中矿物质成分比其它蔬菜更全面，钙、磷、铁、钾、锌、锰等含量都很丰富，比同科属的菜花高出很多。
西兰花的抗癌作用是近些年来西方国家及日本科学家研究的重要内容。日本国家癌症研究中心公布的抗癌蔬菜排行榜上，西兰花名列前茅。美国《营养学》杂志上，也刊登了西兰花能够有效预防前列腺癌的研究成果。西兰花中含有硫代葡萄糖苷，其在黑芥子酶的催化下水解为萝卜硫素，这是西兰花中具有抗癌作用的关键物质。除了硫代葡萄糖苷以外，西兰花还含有丰富的维生素C，能增强肝脏的解毒能力，提高机体免疫力；另外还含有一定量的类黄酮物质，对高血压、心脏病有预防和调节的功效。此外，西兰花属于高纤维蔬菜，能有效降低肠胃对葡萄糖的吸收，进而降低血糖，有效控制糖尿病患者的病情。
由上述可知，西兰花中所含有的丰富多样的营养成分是其具有健康促进作用的重要原因，但人们日常食用西兰花的量不足，另外蒸煮的方式也破坏了植物内源的黑芥子酶，抑制了硫代葡萄糖苷转化生成萝卜硫素、二吲哚甲烷等有重要生物活性的成分，致使常规的食用方式对机体的健康难有大的裨益。因此，目前极需开发一种西兰花多种营养成分的富集工艺，而不是单一成分的提取工艺。利用该工艺生产的产品能够达到具有调节人体营养平衡、促进机体健康的作用。
目前，市场上出售的西兰花粉主要是将西兰花直接进行冷冻干燥、再粉碎加工得到的西兰花粉末，该蔬菜粉末在水中的分散性及溶解性都很差。此外，直接冻干这种处理方式未能起到富集西兰花中多种营养成分的作用，并 且冻干会使黑芥子酶失活，使硫代葡萄糖苷无法转化为萝卜硫素等活性成分。针对该情况，专利申请CN201210258522.2公开了一种通过喷雾干燥制备得到西兰花蔬菜粉的方法，该发明包括如下步骤：原料预处理、漂烫护色、沥水、打浆、均质和喷雾干燥。此加工方法提高了西兰花蔬菜粉的分散性和溶解性，有利于添加到其他食品中而不产生口腔粗糙感。但是由该方法得到的西兰花蔬菜粉没达到富集西兰花营养成分的效果，也没有黑芥子酶催化过程，难以形成对人体有益的萝卜硫素等活性成分。
对于上述问题，专利申请CN201310281689.5公开了一种西兰花浓缩粉的提取制备方法，依次按照以下步骤进行：(1)取西兰花洗净，除去叶子等杂物，切碎；(2)加入大量乙醇进提取；(3)过滤，收集提取液；(4)真空低温干燥；(5)粉碎；(6)封装保存。本法收集的西兰花提取液可将西兰花中有效成分提取完全，并使维生素C等易氧化成分长期保存不变质。该方法虽然对西兰花中的营养成分进行了浓缩，但该方法也没有包括黑芥子酶催化过程，因此生成的萝卜硫素及二吲哚甲烷的量很少；而且在提取过程中使用了大量乙醇，一方面不仅大大提高了其成本而且增加了生产的危险性，另外一方面是提取后的废渣很难再利用，将对生态环境造成污染。
专利申请CN201310126916.7公开了一种西兰花下脚料提取物及其制备方法，该方法包括(1)将西兰花打浆后进行超声波辅助酶解；(2)对所得西兰花浆液中的萝卜硫素和黄酮类化合物进行提取。该发明的特点是将复合酶制剂、超声波辅助萃取和微波辅助萃取应用于西兰花下脚料中有效成分的提取，大大提高了西兰花下脚料的附加值。但该方法引入了外源酶，会对西兰花内在的活性营养成分带来潜在的影响；同时该方法也使用了大量的乙醇，增加了成本，不利于废渣的综合利用。
发明内容
针对上述出现的问题，本发明提供了一种制备西兰花孵育粉的方法。该方法首先对西兰花中的营养成分进行了浓缩；再利用西兰花自身的酶系统，实现了将硫代葡萄糖苷孵育转化为抗肿瘤成分萝卜硫素、二吲哚甲烷等。整个工艺几乎没有废物排放，达到绿色生产标准。
本发明的一个方面提供了一种西兰花孵育粉的制备方法，包括：
(1)将西兰花的茎、叶和花球三种器官中的至少一种器官进行粉碎；
(2)粉碎后的西兰花经过匀浆；
(3)将得到的匀浆液进行孵育；
(4)将孵育后的匀浆液经过压榨得到西兰花压榨汁；
(5)所得的压榨汁经过干燥后得到西兰花孵育粉。
其中，步骤(3)所述孵育的孵育时间为10-240分钟，优选20-120分钟，更优选30-60分钟。
其中，步骤(3)所述孵育的孵育温度为10-60℃，优选20-40℃，更优选20-30℃。
其中，步骤(5)所述的干燥包括冷冻干燥或喷雾干燥。
本发明的另一方面提供了一种西兰花压榨汁的制备方法，所述方法包括：
(1)将西兰花的茎、叶和花球三种器官中的至少一种器官进行粉碎；
(2)粉碎后的西兰花经过匀浆；
(3)将得到的匀浆液进行孵育；
(4)将孵育后的匀浆液经过压榨得到西兰花压榨汁。
其中，步骤(3)所述孵育的孵育时间为10-240分钟，优选20-120分钟，更优选30-60分钟。
其中，步骤(3)所述孵育的孵育温度为10-60℃，优选20-40℃，更优选20-30℃。
本发明的第三个方面涉及根据本发明的方法制备得到的西兰花孵育粉或西兰花压榨汁。
本发明的第四个方面涉及一种组合物，包括本发明的西兰花孵育粉或西兰花压榨汁，所述组合物为食品、保健品或药品，用于降血脂、降血压、抗癌、降血糖和/或提高免疫力。
本发明的第五个方面涉及所述西兰花孵育粉或西兰花压榨汁用于制备具有降血脂、降血压、抗癌、降血糖和/或提高免疫力作用的食品、保健品及药品中的用途，其中，所述抗癌作用中的癌包括：肺癌、前列腺癌、结肠癌、乳腺癌。
本发明的第六个方面涉及所述西兰花孵育粉或西兰花压榨汁用于降血脂、降血压、抗癌、降血糖和/或提高免疫力的用途；优选地，所述的抗癌中的癌包括：肺癌、前列腺癌、结肠癌或乳腺癌。
西兰花中含有黑芥子酶，其在孵育过程中可以将西兰花中的硫代葡萄糖苷酶解生成萝卜硫素；另外通过孵育还可以将西兰花中的其它营养成分如粗多糖、二吲哚甲烷及维生素C等更充分地提取出来。本发明方法简单易行，可操作性强，得到的西兰花孵育粉的营养价值高而且其分散性及水溶性较好，可用于食品、保健品及药品的加工生产中。
具体实施方式
以下实施例是对本发明的进一步说明但不限制本发明。
以下各实施例中，如无特殊说明，所使用的试剂、仪器都是本领域常用试剂、仪器，可以从化学或生物制品/制剂公司购买；以下实施例中使用的方法都是本领域常规方法，本领域技术人员根据现有技术或生产商提供的操作手册可以毫无疑义地知道这些实验的操作过程并获得相应结果。
本发明实施例制备得到的西兰花孵育粉营养成分的分析方法如下：
蛋白含量：食品安全国家标准食品中蛋白质的测定(GB 5009.5-2010)。
粗多糖含量：苯酚硫酸法。
双吲哚甲烷含量(高效液相色谱法)：色谱柱，依利特ODS2-C18(4.6×250mm，5μm)；柱温，25℃；紫外检测波长，226nm；流速，1.0mL/min；进样量，10μL；进样盘温度，2～8℃；流动相，0.01M磷酸二氢钾水溶液：乙腈＝50:50。
维生素C含量(电位滴定法：氧化还原指示电极)：取本品约1.0g，精密称定，置于烧杯中，加新沸过的冷水50mL与稀醋酸5mL使其溶解，超声30min后滴定；用碘滴定液(0.05mol/L)滴定，电位指示法指示终点。每1mL碘滴定液(0.05mol/L)相当于8.806mg的C6H8O6。
萝卜硫素含量(高效液相色谱法)：色谱柱，依利特ODS2-C18(4.6×250mm，5μm)；柱温，25℃；紫外检测波长，240nm；流速，1.0ml/min；进样量，10μl；进样盘温度，2～8℃；流动相，水：乙腈＝20:80。
实施例1
将6株整株西兰花(包括茎、叶、花球)用粉碎机将其粉碎后匀浆，称取等量的6份匀浆液转移至烧杯中，然后放入20℃水浴中分别孵育10、20、30、60、120和240分钟，然后用纱布过滤(300目)(即压榨)得滤液，滤液放入冷冻干燥机中干燥得到西兰花孵育粉。对所得产品中的营养成分进行测定，所得结果如表1所示，各营养成分均衡。
表1
孵育时间/分钟 10 20 30 60 120 240
蛋白质含量(％) 28 30 29 30 30 32
粗多糖含量(％) 2.3 2.5 2.8 3.2 2.9 2.7
二吲哚甲烷(ppm) 0.8 1.2 1.1 0.8 0.8 0.7
维生素C(％) 0.4 0.3 0.3 0.3 0.3 0.2
萝卜硫素(％) 0.022 0.035 0.039 0.051 0.055 0.058
实施例2
将6株整株西兰花用粉碎机将其粉碎后匀浆，称取等量的6份匀浆液转移至烧杯中，然后分别放入10、20、30、40、50和60℃水浴中孵育60分钟后用纱布过滤(300目)(即压榨)得滤液，将滤液放入冷冻干燥机中干燥得到西兰花孵育粉。对所得产品中的营养成分进行测定，所得结果如表2所示。
表2
温度/℃ 10 20 30 40 50 60
蛋白质含量(％) 27 30 31 31 32 28
粗多糖含量(％) 2.5 3.1 3.3 3.2 3.2 3.0
二吲哚甲烷(ppm) 1.5 2.6 2.8 2.5 2.1 2.0
维生素C(％) 0.4 0.3 0.3 0.3 0.2 0.2
萝卜硫素(％) 0.047 0.052 0.058 0.049 0.041 0.032
实施例3
将整株西兰花分为花球、茎和叶三部分，另取整株西兰花一株，然后分别用粉碎机将其打碎。称取等量的整株西兰花碎末、花球碎末、茎碎末和叶碎末并转入烧杯中，然后放入20℃水浴中孵育60分钟，然后用纱布过滤(300目)(即压榨)得滤液，滤液放入冷冻干燥机中干燥得到西兰花孵育粉。对所得产品中的营养成分进行测定，所得结果如表3所示。
表3
西兰花部位 整株 花球 茎 叶
蛋白质含量(％) 32 35 38 24
粗多糖含量(％) 1.3 1.3 0.4 1.4
二吲哚甲烷(ppm) 0.7 1.5 0.3 0.6
维生素C(％) 0.2 0.2 0.2 0.3
萝卜硫素(％) 0.057 0.074 0.038 0.017
实施例4
将10kg整株西兰花用粉碎机将其打碎，然后室温(25℃)孵育60分钟后用纱布过滤(300目)，得滤液5.0L，滤液通过喷雾干燥(BUCHI，小型喷雾干燥仪B-290)得到260g西兰花孵育粉，其蛋白质，粗多糖，二吲哚甲烷、维生素C和萝卜硫素的含量分别为30％，3.3％，2.9ppm、0.3％与0.062％。
实施例5
将实施例3中茎、叶碎末孵育后过滤所得滤渣经过烘干(上海博迅实业有限公司医疗设备厂，电热恒温鼓风干燥箱GZX-9140MBE型)后得到西兰花烘干茎叶粉，经检测其粗蛋白含量为22％(饲料中粗蛋白测定方法，GB/T 6432-1994)，具有利用价值，避免物料浪费。

Claims (11)

  1. 一种西兰花压榨汁的制备方法，其特征在于，所述方法包括：
    (1)将西兰花的茎、叶和花球三种器官中的至少一种器官进行粉碎；
    (2)粉碎后的西兰花经过匀浆；
    (3)将得到的匀浆液进行孵育；
    (4)将孵育后的匀浆液经过压榨得到西兰花压榨汁。
  2. 根据权利要求1所述的方法，其特征在于，步骤(3)所述孵育的孵育时间为10-240分钟，优选20-120分钟，更优选30-60分钟。
  3. 根据权利要求1或2所述的方法，其特征在于，步骤(3)所述孵育的孵育温度为10-60℃，优选20-40℃，更优选20-30℃。
  4. 一种西兰花孵育粉的制备方法，其特征在于，所述方法包括：
    (1)将西兰花的茎、叶和花球三种器官中的至少一种器官进行粉碎；
    (2)粉碎后的西兰花经过匀浆；
    (3)将得到的匀浆液进行孵育；
    (4)将孵育后的匀浆液经过压榨得到西兰花压榨汁；
    (5)所得的压榨汁经过干燥后得到西兰花孵育粉。
  5. 根据权利要求4所述的方法，其特征在于，步骤(3)所述孵育的孵育时间为10-240分钟，优选20-120分钟，更优选30-60分钟。
  6. 根据权利要求4或5所述的方法，其特征在于，步骤(3)所述孵育的孵育温度为10-60℃，优选20-40℃，更优选20-30℃。
  7. 根据权利要求4-6任一项所述的方法，其特征在于，步骤(5)所述的干燥包括冷冻干燥或喷雾干燥。
  8. 根据权利要求1-3任一项所述的方法制备得到的西兰花压榨汁或根据权利要求4-7任一项所述的方法制备得到的西兰花孵育粉。
  9. 一种组合物，包括权利要求8所述的西兰花压榨汁或西兰花孵育粉；优选地，所述组合物为食品、保健品或药品。
  10. 根据权利要求8所述的西兰花压榨汁或西兰花孵育粉在制备具有降血脂、降血压、抗癌、降血糖和/或提高免疫力作用的食品、保健品或药品中的用途；优选地，所述的抗癌作用中的癌包括：肺癌、前列腺癌、结肠癌或乳腺癌。
  11. 根据权利要求8所述的西兰花压榨汁或西兰花孵育粉在降血脂、降血压、抗癌、降血糖和/或提高免疫力中的用途；优选地，所述的抗癌中的癌包括：肺癌、前列腺癌、结肠癌或乳腺癌。
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CN109527283A (zh) * 2018-10-12 2019-03-29 齐齐哈尔大学 一种富含萝卜硫素的十字花科植物饮料及其制备方法
CN111280443A (zh) * 2020-03-24 2020-06-16 浙江劢康生物科技有限公司 一种具有调脂减肥的保健食品及其制备方法
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1405316A (zh) * 2001-09-10 2003-03-26 新功能食品创作技术研究组合 由十字花科植物制备异硫氰酸酯类的方法
US20060127996A1 (en) * 2004-12-14 2006-06-15 Johns Hopkins University Method of extraction of isothiocyanates into oil from glucosinolate-containing plants and method of producing products with oil containing isothiocyanates extracted from glucosinolate-containing plants
DE102005033616A1 (de) * 2005-07-19 2007-01-25 Biopro Ag Biological Products Verfahren zur Herstellung von Extrakten aus Brassica-Arten und ihre Verwendung
CN101066148A (zh) * 2007-06-08 2007-11-07 重庆尚蔬坊饮料食品有限公司 复合蔬果汁
CN101993480A (zh) * 2009-08-12 2011-03-30 中国农业科学院饲料研究所 一种西兰花叶蛋白及其制备方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101548760A (zh) * 2009-04-22 2009-10-07 宁波海逸生物科技有限公司 一种富含Glucoraphanin硫代葡萄糖苷的西兰花浓缩粉的制备工艺
CN102586352B (zh) * 2012-01-16 2014-02-26 南京农业大学 一种芸薹属蔬菜种子中萝卜硫素富集与提取方法
CN102763816A (zh) * 2012-07-25 2012-11-07 浙江万里学院 一种西兰花蔬菜粉的喷雾干燥加工方法

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1405316A (zh) * 2001-09-10 2003-03-26 新功能食品创作技术研究组合 由十字花科植物制备异硫氰酸酯类的方法
US20060127996A1 (en) * 2004-12-14 2006-06-15 Johns Hopkins University Method of extraction of isothiocyanates into oil from glucosinolate-containing plants and method of producing products with oil containing isothiocyanates extracted from glucosinolate-containing plants
DE102005033616A1 (de) * 2005-07-19 2007-01-25 Biopro Ag Biological Products Verfahren zur Herstellung von Extrakten aus Brassica-Arten und ihre Verwendung
CN101066148A (zh) * 2007-06-08 2007-11-07 重庆尚蔬坊饮料食品有限公司 复合蔬果汁
CN101993480A (zh) * 2009-08-12 2011-03-30 中国农业科学院饲料研究所 一种西兰花叶蛋白及其制备方法

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