WO2016194797A1 - 被検対象の検出方法並びにそのための免疫測定器具及びモノクローナル抗体 - Google Patents
被検対象の検出方法並びにそのための免疫測定器具及びモノクローナル抗体 Download PDFInfo
- Publication number
- WO2016194797A1 WO2016194797A1 PCT/JP2016/065683 JP2016065683W WO2016194797A1 WO 2016194797 A1 WO2016194797 A1 WO 2016194797A1 JP 2016065683 W JP2016065683 W JP 2016065683W WO 2016194797 A1 WO2016194797 A1 WO 2016194797A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- antibody
- immunoassay
- antigen
- antigens
- monoclonal antibody
- Prior art date
Links
- 238000003018 immunoassay Methods 0.000 title claims abstract description 55
- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 238000012360 testing method Methods 0.000 title claims abstract description 30
- 239000000427 antigen Substances 0.000 claims abstract description 104
- 102000036639 antigens Human genes 0.000 claims abstract description 103
- 108091007433 antigens Proteins 0.000 claims abstract description 103
- 238000000034 method Methods 0.000 claims abstract description 57
- 238000009739 binding Methods 0.000 claims abstract description 15
- 239000012634 fragment Substances 0.000 claims abstract description 13
- 241000202934 Mycoplasma pneumoniae Species 0.000 claims description 20
- 101001105440 Chlamydomonas reinhardtii Photosystem I reaction center subunit IV, chloroplastic Proteins 0.000 claims description 11
- 101000846901 Drosophila melanogaster Fat-body protein 1 Proteins 0.000 claims description 10
- 239000007790 solid phase Substances 0.000 claims description 10
- 238000003317 immunochromatography Methods 0.000 claims description 6
- 230000035945 sensitivity Effects 0.000 abstract description 17
- 239000000523 sample Substances 0.000 description 25
- 239000000463 material Substances 0.000 description 15
- 230000002725 anti-mycoplasma Effects 0.000 description 14
- 238000010521 absorption reaction Methods 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 10
- 239000012528 membrane Substances 0.000 description 9
- 210000004027 cell Anatomy 0.000 description 8
- 239000002245 particle Substances 0.000 description 7
- 238000002965 ELISA Methods 0.000 description 6
- 210000000746 body region Anatomy 0.000 description 6
- 210000004408 hybridoma Anatomy 0.000 description 6
- 239000003550 marker Substances 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 5
- 239000000020 Nitrocellulose Substances 0.000 description 5
- 238000011161 development Methods 0.000 description 5
- 230000002163 immunogen Effects 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 229920001220 nitrocellulos Polymers 0.000 description 5
- 108090000765 processed proteins & peptides Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 230000009257 reactivity Effects 0.000 description 5
- 241000699670 Mus sp. Species 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- 238000005259 measurement Methods 0.000 description 4
- 229920000136 polysorbate Polymers 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 235000018102 proteins Nutrition 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- 206010003445 Ascites Diseases 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 239000004793 Polystyrene Substances 0.000 description 3
- 238000005194 fractionation Methods 0.000 description 3
- 102000037865 fusion proteins Human genes 0.000 description 3
- 108020001507 fusion proteins Proteins 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 229920002223 polystyrene Polymers 0.000 description 3
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 102000005720 Glutathione transferase Human genes 0.000 description 2
- 108010070675 Glutathione transferase Proteins 0.000 description 2
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 2
- 241000204003 Mycoplasmatales Species 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 102000002933 Thioredoxin Human genes 0.000 description 2
- 238000001042 affinity chromatography Methods 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 229920002678 cellulose Polymers 0.000 description 2
- 239000001913 cellulose Substances 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 239000003365 glass fiber Substances 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 239000004745 nonwoven fabric Substances 0.000 description 2
- XOJVVFBFDXDTEG-UHFFFAOYSA-N pristane Chemical compound CC(C)CCCC(C)CCCC(C)CCCC(C)C XOJVVFBFDXDTEG-UHFFFAOYSA-N 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 238000011002 quantification Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 108060008226 thioredoxin Proteins 0.000 description 2
- 229940094937 thioredoxin Drugs 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 229920002799 BoPET Polymers 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 101000605534 Homo sapiens Prostate-specific antigen Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 102100038358 Prostate-specific antigen Human genes 0.000 description 1
- 241000606701 Rickettsia Species 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- 101710120037 Toxin CcdB Proteins 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000006096 absorbing agent Substances 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 235000011130 ammonium sulphate Nutrition 0.000 description 1
- 210000000628 antibody-producing cell Anatomy 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 150000001718 carbodiimides Chemical class 0.000 description 1
- 229920002301 cellulose acetate Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000012470 diluted sample Substances 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000001641 gel filtration chromatography Methods 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000012744 immunostaining Methods 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000013618 particulate matter Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 229920002704 polyhistidine Polymers 0.000 description 1
- 229920001470 polyketone Polymers 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 102220129022 rs202069145 Human genes 0.000 description 1
- 238000005185 salting out Methods 0.000 description 1
- 235000020183 skimmed milk Nutrition 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 235000011152 sodium sulphate Nutrition 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 229920001059 synthetic polymer Chemical class 0.000 description 1
- -1 test tubes Substances 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/577—Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
- C07K16/1253—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Mycoplasmatales, e.g. Pleuropneumonia-like organisms [PPLO]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
- G01N33/56933—Mycoplasma
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/30—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Mycoplasmatales, e.g. Pleuropneumonia-like organisms [PPLO]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2470/00—Immunochemical assays or immunoassays characterised by the reaction format or reaction type
- G01N2470/04—Sandwich assay format
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Food Science & Technology (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pulmonology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Plant Pathology (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
1.マイコプラズマ・ニューモニエ抗原の調製
マイコプラズマ・ニューモニエを培養し、その培養液を60℃、30分間の熱処理で不活化したものを用いた。
1.のマイコプラズマ不活化抗原をBALB/cマウスに免疫し、一定期間飼育したマウスから脾臓を摘出し、ケラーらの方法(Kohler et al., Nature, vol, 256, p495-497(1975))によりマウスミエローマ細胞(P3×63)と融合した。得られた融合細胞(ハイブリドーマ)を、37℃インキュベーター中で維持し、マイコプラズマ・ニューモニエP1抗原を固相したプレートとマイコプラズマ・ニューモニエP30抗原を固相したプレートを用いたELISAにより上清の抗体活性を確認しながら細胞の純化(単クローン化)を行った。
実施例1で得た各モノクローナル抗体とマイコプラズマ・ニューモニエとの反応性をウエスタンブロッティングで確認した。マイコプラズマ・ニューモニエはPPLO Brothで培養し、その培養液をサンプルとした。
1.抗マイコプラズマ・ニューモニエ抗体のニトロセルロースメンブレンへの固定化
実施例1で作製した抗体を1.0mg/mLになるように精製水で希釈した液及び抗マウスIgG抗体を準備し、PETフィルムで裏打ちされたニトロセルロースメンブレンのサンプルパッド側に抗体、吸収体側に抗マウスIgG抗体をそれぞれ線状に塗布した。その後、ニトロセルロースメンブレンを45℃、30分間乾燥させ、抗マイコプラズマ・ニューモニエ抗体固定化メンブレンを得た。本実施例において抗体固定化メンブレンと呼ぶ。
実施例1で作製した抗体を1.0mg/mLになるように精製水で希釈し、これに着色ポリスチレン粒子を0.1%になるように加え、攪拌後、カルボジイミドを1%になるように加え、さらに攪拌する。遠心操作により上清を除き、50mM Tris(pH9.0)、3%BSAに再浮遊し、抗マイコプラズマ・ニューモニエ抗体結合着色ポリスチレン粒子を得た。本実施例において、抗体固定化粒子と呼ぶ。
1で作製した抗体固定化メンブレンと他部材(バッキングシート、吸収帯、サンプルパッド)とを貼り合せて5mm幅に切断し、マイコプラズマ・ニューモニ試験片とした(図2参照)。これらを本実施例において、試験片と呼ぶ。
P1、P30及びP1とP30を測定する免疫測定器具の感度比較
実施例1で得られたモノクローナル抗体を表2の組み合わせで実施例3の手順によりP1試験片(比較例1)、P30試験片(比較例2)、P1-P30試験片(実施例4)の3種類作製した。
P1-P30試験片の使用抗体をP1-P30AとP1-P30Bの組み合わせで実施例4と同様に試験した。その結果、P1-P30Aのみの組み合わせ(実施例4)と比較して2~4倍優れた感度を示した。
2 標識体部
3 検出領域
4 サンプルパッド
5 吸収帯
6 バッキングシート
Claims (11)
- 被検対象の検出を可能とする2種類以上の抗原が存在する被検対象の検出方法であって、前記2種類以上の抗原を認識するモノクローナル抗体又はその抗原結合性断片を用いた免疫測定方法により行う、前記被検対象の検出方法。
- 前記モノクローナル抗体又はその抗原結合性断片が標識または固相の少なくともいずれか一方に使用される、前記免疫測定方法がサンドイッチ法である請求項1記載の方法。
- 前記免疫測定方法がイムノクロマトグラフィー法である請求項2記載の方法。
- 前記モノクローナル抗体により認識される前記2種類以上の抗原が複合体を形成している請求項1~3のいずれか1項に記載の方法。
- 前記被検対象がマイコプラズマ・ニューモニエである請求項1~4のいずれか1項に記載の方法。
- 前記2種類以上の抗原がP1タンパク質とP30タンパク質である請求項5記載の方法。
- 請求項1記載の方法を行うための免疫測定器具であって、前記モノクローナル抗体又はその抗原結合性断片が標識または固相の少なくともいずれか一方に使用される、免疫測定器具。
- イムノクロマト試験片である請求項7記載の免疫測定器具。
- 前記被検対象がマイコプラズマ・ニューモニエである請求項7又は8記載の免疫測定器具。
- 前記2種類以上の抗原がP1タンパク質とP30タンパク質である請求項9記載の免疫測定器具。
- マイコプラズマ・ニューモニエ由来のP1タンパク質とP30タンパク質に対して特異的に反応するモノクローナル抗体。
Priority Applications (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP16803232.4A EP3306319B1 (en) | 2015-05-29 | 2016-05-27 | Test object detection method, and immunoassay instrument and monoclonal antibody for same |
CN201680031403.XA CN107683412A (zh) | 2015-05-29 | 2016-05-27 | 被检对象的检测方法以及用于该方法的免疫测定仪器和单克隆抗体 |
MYPI2017704569A MY194325A (en) | 2015-05-29 | 2016-05-27 | Test object detection method, and immunoassay instrument and monoclonal antibody for same |
US15/577,542 US20180164316A1 (en) | 2015-05-29 | 2016-05-27 | Test object detection method, and immunoassay instrument and monoclonal antibody for same |
KR1020177037381A KR102569269B1 (ko) | 2015-05-29 | 2016-05-27 | 피검 대상의 검출 방법 및 그것을 위한 면역 측정 기구 및 모노클로널 항체 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2015-110585 | 2015-05-29 | ||
JP2015110585A JP6655302B2 (ja) | 2015-05-29 | 2015-05-29 | 被検対象の検出方法並びにそのための免疫測定器具及びモノクローナル抗体 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2016194797A1 true WO2016194797A1 (ja) | 2016-12-08 |
Family
ID=57440534
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2016/065683 WO2016194797A1 (ja) | 2015-05-29 | 2016-05-27 | 被検対象の検出方法並びにそのための免疫測定器具及びモノクローナル抗体 |
Country Status (7)
Country | Link |
---|---|
US (1) | US20180164316A1 (ja) |
EP (1) | EP3306319B1 (ja) |
JP (1) | JP6655302B2 (ja) |
KR (1) | KR102569269B1 (ja) |
CN (1) | CN107683412A (ja) |
MY (1) | MY194325A (ja) |
WO (1) | WO2016194797A1 (ja) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021206079A1 (ja) | 2020-04-08 | 2021-10-14 | デンカ株式会社 | マイコプラズマ・ニューモニエの免疫測定方法及び免疫測定器具 |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111548423B (zh) * | 2020-06-04 | 2021-11-30 | 珠海丽珠试剂股份有限公司 | 肺炎支原体融合抗原及其制备方法和应用 |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988004669A1 (en) * | 1986-12-15 | 1988-06-30 | Mitsui Toatsu Chemicals, Inc. | Human monoclonal antibody and drug for prophylaxis and treatment of infectious diseases comprising same as effective ingredient |
JP2004258024A (ja) * | 2003-02-03 | 2004-09-16 | Toyobo Co Ltd | 皮膚糸状菌の検出方法、検出用試薬および抗原性賦活化方法 |
JP2011079830A (ja) * | 2006-06-14 | 2011-04-21 | M Bio Tech Kk | マイコプラズマ・ニューモニエのグリセロ型糖脂質抗原 |
JP2011220931A (ja) * | 2010-04-13 | 2011-11-04 | Sekisui Medical Co Ltd | マイコプラズマ・ニューモニエ抗原測定の検出方法 |
WO2015025968A1 (ja) * | 2013-08-23 | 2015-02-26 | 株式会社ビーエル | マイコプラズマ・ニューモニエの免疫学的検出法およびキット |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR20080074231A (ko) * | 2000-06-29 | 2008-08-12 | 아보트 러보러터리즈 | 이중 특이성 항체 및 이의 제조 및 사용 방법 |
WO2003012445A1 (en) * | 2001-07-30 | 2003-02-13 | Quest Diagnostics Investments Incorporated | Methods and kits for detecting ita in a biological sample |
US8323966B2 (en) * | 2009-06-25 | 2012-12-04 | Geron Corporation | Differentiated pluripotent stem cell progeny depleted of extraneous phenotypes |
CN102928606B (zh) * | 2012-11-16 | 2015-10-07 | 武汉明德生物科技股份有限公司 | 多抗体标记的降钙素原快速检测试剂盒 |
CN103275196B (zh) * | 2013-06-24 | 2015-09-30 | 武汉市长立生物技术有限责任公司 | 一种肺炎支原体重组抗原及其制备方法和应用 |
CN104198703B (zh) * | 2014-08-18 | 2015-12-30 | 湖北工业大学 | 人肺炎支原体金标银染免疫层析检测试剂盒及其制备方法和应用 |
-
2015
- 2015-05-29 JP JP2015110585A patent/JP6655302B2/ja active Active
-
2016
- 2016-05-27 CN CN201680031403.XA patent/CN107683412A/zh active Pending
- 2016-05-27 WO PCT/JP2016/065683 patent/WO2016194797A1/ja active Application Filing
- 2016-05-27 EP EP16803232.4A patent/EP3306319B1/en active Active
- 2016-05-27 US US15/577,542 patent/US20180164316A1/en not_active Abandoned
- 2016-05-27 MY MYPI2017704569A patent/MY194325A/en unknown
- 2016-05-27 KR KR1020177037381A patent/KR102569269B1/ko active IP Right Grant
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1988004669A1 (en) * | 1986-12-15 | 1988-06-30 | Mitsui Toatsu Chemicals, Inc. | Human monoclonal antibody and drug for prophylaxis and treatment of infectious diseases comprising same as effective ingredient |
JP2004258024A (ja) * | 2003-02-03 | 2004-09-16 | Toyobo Co Ltd | 皮膚糸状菌の検出方法、検出用試薬および抗原性賦活化方法 |
JP2011079830A (ja) * | 2006-06-14 | 2011-04-21 | M Bio Tech Kk | マイコプラズマ・ニューモニエのグリセロ型糖脂質抗原 |
JP2011220931A (ja) * | 2010-04-13 | 2011-11-04 | Sekisui Medical Co Ltd | マイコプラズマ・ニューモニエ抗原測定の検出方法 |
WO2015025968A1 (ja) * | 2013-08-23 | 2015-02-26 | 株式会社ビーエル | マイコプラズマ・ニューモニエの免疫学的検出法およびキット |
Non-Patent Citations (2)
Title |
---|
GUTTIKONDA, SUJATHA ET AL.: "Monospecific and bispecific antibodies against E. coli 0157 for diagnostics", JOURNAL OF IMMUNOLOGICAL METHODS, vol. 327, no. 1-2, 2007, pages 1 - 9, XP022266385 * |
OWAIS, MOHAMMAD ET AL.: "An Alternative Chemical Redox Method for the Production of Bispecific Antibodies: Implication in Rapid Detection of Food Borne Pathogens", PLOS ONE, vol. 9, no. 3, 2014, pages 1 - 13, XP055333846 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021206079A1 (ja) | 2020-04-08 | 2021-10-14 | デンカ株式会社 | マイコプラズマ・ニューモニエの免疫測定方法及び免疫測定器具 |
KR20220164733A (ko) | 2020-04-08 | 2022-12-13 | 덴카 주식회사 | 마이코플라즈마 뉴모니에의 면역 측정 방법 및 면역 측정 기구 |
JP7372196B2 (ja) | 2020-04-08 | 2023-10-31 | デンカ株式会社 | マイコプラズマ・ニューモニエの免疫測定方法及び免疫測定器具 |
Also Published As
Publication number | Publication date |
---|---|
EP3306319A4 (en) | 2019-01-09 |
KR20180020992A (ko) | 2018-02-28 |
KR102569269B1 (ko) | 2023-08-22 |
JP6655302B2 (ja) | 2020-02-26 |
EP3306319A1 (en) | 2018-04-11 |
US20180164316A1 (en) | 2018-06-14 |
CN107683412A (zh) | 2018-02-09 |
JP2016223913A (ja) | 2016-12-28 |
EP3306319B1 (en) | 2020-03-25 |
MY194325A (en) | 2022-11-28 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
KR102227648B1 (ko) | B형 인플루엔자 바이러스의 측정 방법 | |
JP2024012473A (ja) | Rsウイルスを検出するための検出用試薬又はキット及びrsウイルスを検出する方法 | |
RU2366663C2 (ru) | Устройство для иммунного анализа с использованием антитела к вирусу гриппа типа b | |
WO2016194797A1 (ja) | 被検対象の検出方法並びにそのための免疫測定器具及びモノクローナル抗体 | |
JP7455947B2 (ja) | アデノウイルスの免疫測定方法及び免疫測定器具 | |
JP2019207248A (ja) | 被検対象の検出方法並びにそのための免疫測定器具及びモノクローナル抗体 | |
JP7372196B2 (ja) | マイコプラズマ・ニューモニエの免疫測定方法及び免疫測定器具 | |
WO2021117862A1 (ja) | 免疫測定方法及び免疫測定器具 | |
WO2021210631A1 (ja) | アデノウイルスの免疫測定方法及び免疫測定器具 | |
JP7436274B2 (ja) | アデノウイルスの免疫測定方法及び免疫測定器具 | |
WO2021095762A1 (ja) | 抗rsウイルスn蛋白質を認識する抗体、並びに該抗体を用いた免疫測定方法及び免疫測定器具 | |
US11912783B2 (en) | Monoclonal antibody against IgM and non-specific reaction inhibitor |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 16803232 Country of ref document: EP Kind code of ref document: A1 |
|
WWE | Wipo information: entry into national phase |
Ref document number: 15577542 Country of ref document: US |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
ENP | Entry into the national phase |
Ref document number: 20177037381 Country of ref document: KR Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2016803232 Country of ref document: EP |