WO2016186312A1 - Dispositif de pcr - Google Patents

Dispositif de pcr Download PDF

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Publication number
WO2016186312A1
WO2016186312A1 PCT/KR2016/003286 KR2016003286W WO2016186312A1 WO 2016186312 A1 WO2016186312 A1 WO 2016186312A1 KR 2016003286 W KR2016003286 W KR 2016003286W WO 2016186312 A1 WO2016186312 A1 WO 2016186312A1
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WIPO (PCT)
Prior art keywords
sample
temperature
heating
temperature control
weight
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PCT/KR2016/003286
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English (en)
Korean (ko)
Inventor
김윤진
조진우
신권우
박지선
Original Assignee
주식회사 대화알로이테크
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Publication of WO2016186312A1 publication Critical patent/WO2016186312A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices

Definitions

  • the present invention relates to a PCR device.
  • DNA amplification is widely used for research and development and diagnostic purposes in the fields of life science, genetic engineering, and medicine.
  • DNA amplification by polymerase chain reaction (PCR) is widely used. It is becoming.
  • the PCR is used to amplify specific DNA sequences in the genome as necessary.
  • the PCR amplifies DNA through a denaturation step, an annealing step, and an extension step.
  • the PCR is performed by a PCR device.
  • the PCR device adjusts the temperature of the sample sample to the step-by-step temperature required for DNA synthesis using one temperature controller. Since the temperature control unit controls the temperature of the sample sample unit, it may take a long time to complete the PCR due to a delay in increasing or decreasing the temperature of the sample sample unit.
  • the PCR apparatus has a plurality of temperature control units having different fixed temperatures, and the sample sample unit may move between the temperature control units.
  • Korean Patent Publication No. 2010-0008476 discloses a technique for moving the sample sample portion between the temperature control portion.
  • the PCR device can reduce the time required to complete the PCR.
  • the sample sample portion moves, it is difficult to detect the accurate result from the sample sample portion using light in the sensor module due to shaking the sample sample portion.
  • Another object of the present invention is to provide a PCR device including a heating paste composition having high heat resistance, small resistance change according to temperature, low specific resistance, and which can be driven with low voltage and low power.
  • the PCR device accommodates a material for synthesizing DNA, the sample sample portion disposed in a fixed state, and arranged to be adjacent to the sample sample portion And at least one planar heating element formed through the exothermic paste composition, and including a temperature control unit configured to adjust the temperature of the sample sample unit to a step-by-step temperature required for DNA synthesis while moving with respect to the sample sample unit in a fixed state.
  • the heating paste composition may include 3 to 6 parts by weight of carbon nanotube particles, 0.5 to 30 parts by weight of carbon nanoparticles, 10 to 30 parts by weight of a mixed binder, 29 to 83 parts by weight of an organic solvent, and a dispersant based on 100 parts by weight of a heat paste composition.
  • the mixed binder is epoxy acrylate, polyvinyl acetal and phenolic resin Is mixed or hexamethylene diisocyanate, polyvinyl acetal and phenolic resin are mixed.
  • the temperature adjusting part may include a first heating region for heating the sample sample portion to a first temperature, a second heating region for heating the sample sample portion to a second temperature lower than the first temperature, and the sample.
  • a third heating region for heating the sample part to a third temperature between the first temperature and the second temperature may be sequentially arranged.
  • the method may further include a sensor module for determining the amplification degree of the DNA by irradiating light to the sample sample unit and determining the DNA.
  • the temperature controller may include a light transmitting region for transmitting light of the sensor module on one side of the first heating region or on one side of the third heating region, and the sensor module may be configured to pass through the light transmitting region. The light can be irradiated.
  • the sensor module may be disposed between the first heating region and the third heating region of the temperature controller to move together with the temperature controller.
  • the temperature control part may be provided between the first heating area and the second heating area, and further include a cooling area for cooling the sample sample part.
  • the temperature controller may further include a heat insulation region between each of the regions to prevent heat transfer between the regions of the temperature controller.
  • the temperature control part may be rotatably supported to adjust the temperature of the sample sample part, and may further include a slip ring for supplying power to the temperature control part.
  • it may further include a housing provided to surround the temperature control part and having an insertion hole for accommodating the sample sample part.
  • the housing may include a guide protrusion on an inner surface including a portion in which the insertion hole is formed, and the temperature control part may be configured to maintain a constant distance from the sample sample part while moving the temperature control part. It may include a guide member extending to contact.
  • the mixed binder may be mixed with 10 to 150 parts by weight of polyvinyl acetal resin, 100 to 500 parts by weight of phenolic resin based on 100 parts by weight of epoxy acrylate or hexamethylene diisocyanate.
  • 0.5 to 5 parts by weight of the silane coupling agent may be further included based on 100 parts by weight of the heating paste composition.
  • the carbon nanotube particles may be multi-walled carbon nanotube particles.
  • the organic solvent is carbitol acetate, butyl carbitol acetate, DBE (dibasic ester), ethyl carbitol, ethyl carbitol acetate, dipropylene glycol methyl ether, cellosolve acetate, butyl cellosolve acetate, butanol And two or more mixed solvents selected from octanol.
  • the planar heating element may be formed by screen printing, gravure printing, or comma coating the heating paste composition on a substrate.
  • the substrate may be a polyimide substrate, glass fiber mat or ceramic glass.
  • the planar heating element may be coated on the top surface of the planar heating element, and may further include a protective layer formed of an organic material including a black pigment such as silica or carbon black.
  • the apparatus may further include a power supply unit configured to supply power to the planar heating element.
  • it has a high heat resistance may have a small resistance change according to the temperature
  • low specific resistance may include a heat paste composition capable of driving at low voltage and low power.
  • FIG. 1 is a side cross-sectional view for explaining a PCR device according to a first embodiment of the present invention.
  • FIG. 2 is a plan view illustrating the temperature control unit shown in FIG. 1.
  • FIG 3 is a side cross-sectional view for explaining a PCR device according to a second embodiment of the present invention.
  • FIG. 4 is a side cross-sectional view for explaining a PCR device according to a third embodiment of the present invention.
  • FIG. 5 is a side cross-sectional view for explaining a PCR device according to a fourth embodiment of the present invention.
  • FIG. 6 is a plan view illustrating the temperature controller and the sensor module illustrated in FIG. 5.
  • FIG. 7 is a side cross-sectional view for explaining a PCR device according to a fifth embodiment of the present invention.
  • FIG. 8 is a cross-sectional view taken along line AA ′ of FIG. 7.
  • FIG. 9 is a side cross-sectional view for explaining a PCR device according to a sixth embodiment of the present invention.
  • FIG. 10 is a plan view illustrating the temperature controller illustrated in FIG. 9.
  • FIG. 11 is an image of a specimen of a planar heating element using a heating paste composition included in a PCR device according to an embodiment of the present invention.
  • FIG. 1 is a side cross-sectional view for explaining a PCR device according to a first embodiment of the present invention
  • Figure 2 is a plan view for explaining the temperature control unit shown in FIG.
  • the PCR device 100 includes a housing 110, a sample sample unit 120, a temperature control unit 130, a sensor module 140, a slip ring 150, and a driving unit 160. It includes.
  • the housing 110 has a hollow cylindrical or box shape.
  • the housing 110 has an insertion hole 112 through which the sample sample part 120 is inserted.
  • the insertion hole 112 may be located at the side of the housing 110.
  • the housing 110 also has a guide protrusion 114 along the inner side circumference.
  • the height of the guide protrusion 114 may be substantially the same as the height of the insertion hole (112).
  • Sample sample unit 120 has a substantially flat plate shape, and houses a material for synthesizing DNA.
  • the sample sample unit 120 may be inserted into and fixed to the insertion hole 112 of the housing 110.
  • the sample sample unit 120 may include a temperature sensor (not shown). The temperature of the sample sample unit 120 may be accurately measured using the temperature sensor. The temperature of the sample sample unit 120 measured by the temperature sensor may be used by the temperature controller 130 to adjust the temperature of the sample sample unit 120.
  • the temperature control unit 130 is provided inside the housing 110 and is disposed to be adjacent to the sample sample unit 120 inserted into and fixed to the housing 110.
  • the temperature control unit 130 includes at least one or more planar heating elements formed through the heating paste composition, and moves with respect to the sample sample unit 120 while the temperature of the sample sample unit 120 is at a stepwise temperature necessary for DNA synthesis. Adjust The heat generating paste composition forming the planar heating element and planar heating element included in the temperature control unit 130 will be described in more detail later.
  • the temperature control unit 130 may include a pair of temperature control members 131 including a planar heating element formed through the heat paste composition.
  • the temperature regulating members 131 have a disc shape, respectively, and are disposed to be parallel to each other above and below the sample sample part 120. In order to prevent the temperature control member 131 from colliding with the sample sample unit 120 when the temperature control members 131 move, the temperature control members 131 are spaced apart from each other by a distance wider than the thickness of the sample sample unit 120.
  • Each temperature regulating member 131 has a first heating region 132, a cooling region 133, a second heating region 134, a third heating region 135, and a light transmitting region 136 along the circumference of the disc. Sequentially located.
  • the temperature control unit 130 heats and cools the sample sample unit 120 while the temperature control members 131 rotate.
  • the first heating area 132 heats the sample sample part 120 to a first temperature
  • the cooling area 133 cools the sample sample part 120
  • the second heating area 134 is a sample sample part ( 120 is heated to a second temperature lower than the first temperature
  • the third heating zone 135 heats the sample sample portion to a third temperature between the first and second temperatures.
  • the first temperature is about 94 to 95 °C
  • the second temperature is about 55 °C
  • the third temperature is preferably about 72 °C, but the first temperature, the second temperature and the second by the user 3
  • the temperature may vary.
  • the cooling region 133 may have a temperature equal to or lower than the second temperature.
  • the sample sample unit 120 may be cooled to the second temperature more quickly. Meanwhile, the cooling region 133 may be omitted as necessary.
  • the first heating region 132, the second heating region 134, and the third heating region 135 may be provided with a thermoelectric element, a light source, a heating coil, and the like.
  • the cooling region 133 may include a thermoelectric element, a heat radiating fin, a cooling coil, a cooling fan, and the like.
  • a temperature sensor may be provided in each of the first heating region 132, the cooling region 133, the second heating region 134, and the third heating region 135.
  • the first heating region 132, the cooling region 133, the second heating region 134, and the third heating region 135 may be adjusted to have a constant temperature.
  • the double-stranded DNA is separated into single-stranded DNA. Then, since the DNA sample is cooled to the second temperature, the DNA strands are partially double-stranded by allowing the single-stranded DNA and the primer to be double-stranded.
  • the primer of the DNA-primer complex can be extended by a polymerase by a polymerase polymerase, and thus, a new single strand having a sequence complementary to the original template DNA. DNA can be replicated.
  • the temperature control member 131 rotates and heats and cools the sample sample part 120, the time required for heating and cooling the sample sample part 120 can be reduced. Therefore, the time required for PCR of the sample sample unit 120 can be shortened.
  • the sample sample unit 120 since the sample sample unit 120 is maintained in a fixed state, the DNA sample is not shaken and maintained in a stable state. Since the shaking of the DNA sample is prevented, the amplification degree and DNA of the DNA can be accurately determined from the sample sample unit 120.
  • the light transmitting region 136 is a region for transmitting the light irradiated from the sensor module 140 to the sample sample unit 120.
  • the light transmitting region 136 may be formed of an empty space, or may be made of a transparent material through which the light may pass.
  • the thermal insulation regions 137 are positioned between the first heating region 132, the cooling region 133, the second heating region 134, the third heating region 135, and the light transmitting region 136.
  • the adiabatic regions 137 are disposed or partially disposed between the first heating region 132, the cooling region 133, the second heating region 134, the third heating region 135, and the light transmitting region 136, respectively. May optionally be placed only between.
  • the thermal insulation regions 137 prevent heat from the first heating region 132, the cooling region 133, the second heating region 134, and the third heating region 135 from being transferred to the adjacent region.
  • the insulation regions 137 may be made of an empty space or made of an insulation material.
  • the temperature controller 130 further includes a fixing member 138 and a pair of guide members 139.
  • the fixing member 138 fixes the central portion of the temperature regulating members 131. Therefore, the temperature regulating members 131 may be maintained in parallel at a predetermined interval.
  • the guide members 139 may be provided at the upper edge of the upper temperature regulating member 131 and the lower edge of the lower temperature regulating member 131, respectively.
  • the guide members 139 have a ring shape.
  • the guide members 139 may be provided to cover the entire upper surface of the upper temperature regulating member 131 and the entire lower surface of the lower temperature regulating member 131.
  • the guide members 139 may have a substantially disc shape.
  • the guide members 139 fix the first heating region 132, the cooling region 133, the second heating region 134, the third heating region 135, the light transmitting region 136, and the thermal insulation region 137. It plays a role.
  • the guide members 139 may protrude from the edge of the temperature control member 131 in a radial direction, that is, in a horizontal direction.
  • the protruding guide members 139 may be supported in contact with the guide protrusion 114 of the housing 110.
  • the thickness of the guide protrusion 114 may be substantially equal to the distance between the guide members 139, and the guide protrusion 114 may be disposed between the guide members 139 to support the guide members 139. Can be.
  • the thickness of the guide protrusion 114 is greater than the gap between the guide members 139, and the guide protrusion 114 has grooves (not shown) at the same height as the height of the guide members 139.
  • the guide protrusion 114 may accommodate the guide members 139 in the grooves to support the guide members 139.
  • the guide members 139 are supported by the guide protrusions 114 even when the temperature regulating members 131 rotate, so that the distance between the temperature regulating members 131 can be kept constant. Therefore, the gap between the temperature adjusting members 131 can be narrowed to prevent the collision with the sample sample part 120.
  • the sensor module 140 is provided inside the housing 110, and determines the DNA amplification degree and the DNA using light.
  • the sensor module 140 may be fixed to the inner side surface of the housing 110.
  • the sensor module 140 irradiates light onto the sample sample unit 120 while the light transmitting region 136 of the temperature controller 130 is positioned above and below the sample sample unit 120, and then the sample sample unit 120. It can receive light transmitted from it.
  • the sensor module 140 may have a light emitting unit disposed above the sample sample unit 120, and a light receiving unit may be disposed below the sample sample unit 120.
  • the light is radiated from the light emitting part of the sensor module 140 to the sample sample part 120 while the light transmitting region 136 of the temperature controller 130 is positioned above and below the sample sample part 120.
  • the light irradiated from the light emitting part passes through the light transmitting region 136 of the upper temperature adjusting member 131, the sample sample part 120, and the light transmitting region 136 of the lower temperature adjusting member 131. do.
  • the upper and lower portions of the part in which the material is accommodated in the sample sample part 120 are transparent, so that the light penetrates the sample sample part 120. It may be limited to the case.
  • the sensor module 140 may be disposed integrally with the light emitting unit and the light receiving unit above or below the sample sample unit 120.
  • the light irradiated from the light emitting part passes through the light transmitting region 136 of the temperature adjusting member 131 to the sample sample part 120, and the light reflected from the sample sample part 120 passes through the temperature adjusting member 131.
  • the light-receiving portion may be received by passing through the light transmission region 136.
  • any one of the upper and lower portions of the portion in which the material is accommodated in the sample sample unit 120 is transparent, so that the light is sampled. It may be limited to the case where the portion 120 does not penetrate.
  • the sample sample unit 120 may further include a reflective member to increase the reflection efficiency of the light entering through the transparent portion.
  • the sensing efficiency of the sensor module 140 may be improved.
  • the distance between the sensor module 140 and the sample sample unit 120 is kept constant, the sensing accuracy of the sensor module 140 can be improved.
  • the temperature of the sample sample unit 120 may be variously set according to the user's selection.
  • the degree of amplification of the sample can be determined.
  • the amplification degree of the DNA sample accommodated in the sample sample unit 120 may be determined using the sensor module 140 while the sample sample unit 120 has a temperature of about 55 ° C.
  • the temperature adjusting member 131 may include the third heating area 135.
  • a fourth heating region for maintaining the second cooling region (not shown) or the sample sample unit 120 at the temperature of about 55 ° C. .
  • the cooling region 133 or the second heating region 134 of the temperature regulating member 131 is further provided without the second cooling region or the fourth heating region.
  • the temperature of the sample sample unit 120 heated to a third temperature of about 72 ° C may be adjusted to about 55 ° C.
  • the sensor module 140 may be omitted in some cases. That is, the PCR device 100 may not include the sensor module 140.
  • Slip ring 150 is provided between the temperature control unit 130, specifically between the fixing member 138 and the housing 110, the sample sample with the temperature control unit 130 while supporting the rotating temperature control unit 130 Power is supplied to heat and cool the unit 120.
  • the slip ring 150 includes a fixing part 152 and a rotating part 154.
  • the fixing part 152 is fixed to the inner lower surface of the housing 110 and has a wire connected to an external power source.
  • the rotating part 154 is provided to be rotatable on the fixing part 152 and is electrically connected to the fixing part 152.
  • the connection between the rotating part 154 and the fixing part 152 is divided into an end type and a hollow type according to the mounting method of the rotating part, and the contact method using carbon graphite or alloy material and the contact method using mercury or liquid metals according to the contact method. Can be divided into a contactless manner.
  • the rotating unit 154 is connected to the fixing member 138 of the temperature control unit 130, and rotates together with the temperature control unit 130.
  • the rotating part 154 has a wire electrically connected to the temperature adjusting members 131 of the temperature adjusting part 130 through the fixing member 138.
  • the rotating unit 154 may serve as the fixing member 138 without providing a separate fixing member 138.
  • the wire of the rotating unit 154 may be directly connected to the temperature control member 131.
  • slip ring 150 Since the slip ring 150 is used, power may be supplied to the temperature controller 130 without twisting the wires even when the temperature controller 130 rotates.
  • the driving unit 160 is provided inside the housing 110 and includes a first heating region 132, a cooling region 133, a second heating region 134, a third heating region 135, and a light transmitting region 136. ) Rotates the temperature controller 130 so that the sample passes through the sample sample 120 in sequence.
  • An example of the driver 160 may be a step motor.
  • the driving unit 160 may be directly connected to the temperature controller 130 to rotate the temperature controller 130.
  • the driving unit 160 may be connected to the fixing member 138.
  • the driving unit 160 may rotate the temperature control unit 130 using a gear (not shown). By using the gear, the position of the driving unit 160 may be changed to minimize the size of the housing 110.
  • FIG 3 is a side cross-sectional view for explaining a PCR device according to a second embodiment of the present invention.
  • the PCR device 200 includes a housing 210, a sample sample unit 220, a temperature controller 230, a sensor module 240, a slip ring 250, and a driver 260. .
  • the temperature control unit 230 includes one temperature control member rather than a pair.
  • the temperature control member may be disposed on one side of the sample sample unit 220. That is, the temperature controller 230 may be disposed below or above the sample sample unit 220.
  • the structure of the temperature controller 230 may be simplified. Therefore, it is possible to reduce the cost required to configure the temperature controller 230, and to reduce the possibility of failure due to the structure of the temperature controller 230.
  • FIG. 4 is a side cross-sectional view for explaining a PCR device according to a third embodiment of the present invention.
  • the PCR apparatus 300 includes a housing 310, a sample sample unit 320, a temperature controller 330, a sensor module 340, a slip ring 350, and a driver 360. .
  • the sample sample part 320, and the temperature control part 330 is substantially the same as that of the PCR device 100 with reference to FIGS. 1 and 2. Omit.
  • Sample sample portion 320 has a tube shape.
  • the sample sample part 320 is inserted through the insertion hole 312 of the housing 310 in a fixed state by the clamp 322, and is fixed to be spaced apart from the temperature control part 330 during PCR.
  • the insertion hole 312 has a size sufficient to allow the clamp 322 of the sample sample part 320 to be inserted.
  • the temperature control unit 330 includes one temperature control member rather than a pair.
  • the temperature control member may be disposed on one side of the sample sample part 320. That is, the temperature controller 130 may be disposed below or above the sample sample unit 320.
  • a ring-shaped groove may be formed in the temperature control member of the temperature control part 330 to accommodate the sample sample part 320 in the form of a tube.
  • the PCR apparatus 300 may perform DNA amplification and discrimination even on the tube sample sample 320.
  • FIG. 5 is a side cross-sectional view for explaining a PCR device according to a fourth embodiment of the present invention
  • FIG. 6 is a plan view for explaining the temperature control unit and the sensor module shown in FIG. 5.
  • the PCR device 400 includes a housing 410, a sample sample part 420, a temperature controller 430, a sensor module 440, a slip ring 450, and a driver 460. It includes.
  • the sensor module 440 may be located between the first heating region 432 and the third heating region 435 in each of the temperature regulating members 431 of the temperature regulating unit 430. That is, the sensor module 440 may be located in the light transmission region without being fixed to the housing 410. Therefore, the sensor module 440 may be disposed closer to the sample sample unit 420. Therefore, the sensor module 440 can more accurately determine the degree of DNA amplification and the DNA type of the sample sample unit 420.
  • FIG. 7 is a side cross-sectional view illustrating a PCR device according to a fifth embodiment of the present invention
  • FIG. 8 is a cross-sectional view taken along line AA ′ of FIG. 7.
  • the PCR apparatus 500 includes a housing 510, a sample sample unit 520, a temperature controller 530, a sensor module 540, a slip ring 550, and a driver 560. It includes.
  • the insertion hole 512 may be located on an upper surface of the housing 510. Therefore, the sample sample part 520 may be inserted in the insertion hole 512 to stand in the vertical direction from the upper side of the housing 510.
  • the sample sample unit 520 may have a tube shape as well as a substantially rectangular flat plate shape.
  • the temperature controller 530 includes a pair of temperature regulating members 531.
  • the temperature regulating members 531 have a hollow cylindrical shape which is approximately open at the top and bottom.
  • the temperature regulating members 531 have different diameters and are arranged in a concentric shape such that the sample sample part 520 is located therebetween.
  • the temperature adjusting members 531 are spaced apart from each other by an interval wider than the thickness of the sample sample unit 520.
  • Each temperature regulating member 531 has a first heating region 532, a cooling region 533, a second heating region 534, a third heating region 535 and a light transmitting region 536 along the circumference of the cylinder. Sequentially located. Insulating regions 537 are positioned between the first heating region 532, the cooling region 533, the second heating region 534, the third heating region 535, and the light transmitting region 536, respectively.
  • the sensor module 540 may be provided at an inner side surface of the housing 510.
  • the temperature controller 530 may include only one of the pair of temperature regulating members 531.
  • the sensor module 540 may not be provided at the inner side of the housing 510 but may be provided at the light transmitting region 536 of the temperature control member 531.
  • FIG. 9 is a side cross-sectional view for explaining a PCR device according to a sixth embodiment of the present invention
  • Figure 10 is a plan view for explaining the temperature control unit shown in FIG.
  • the PCR apparatus 600 includes a housing 610, a sample sample unit 620, a temperature controller 630, a sensor module 640, and a driver 660.
  • the housing 610 has a hollow box shape.
  • the housing 610 has an insertion hole 612 for inserting the sample sample portion 620 on the side.
  • the housing 610 has a guide protrusion 614 along the inner wall of the side surface where the insertion hole 612 is formed.
  • the height of the guide protrusion 614 may be substantially the same as the height of the insertion hole 612.
  • the thickness of the guide protrusion 614 may be substantially the same as the gap between the guide members 639 to be described later.
  • the sample sample portion 620 has a substantially flat plate shape and houses a material for synthesizing DNA.
  • the sample sample unit 620 may be inserted into and fixed to the insertion hole 612 of the housing 610.
  • the temperature controller 630 is provided inside the housing 610 and is disposed to be adjacent to the sample sample part 620 inserted into and fixed to the housing 610.
  • the temperature controller 630 adjusts the temperature of the sample sample 620 to a step-by-step temperature required for DNA synthesis while moving relative to the sample sample 620.
  • the temperature controller 630 includes a pair of temperature regulating members 631.
  • the temperature regulating members 631 have a substantially rectangular flat plate shape, and are disposed to be parallel to each other above and below the sample sample part 620.
  • the temperature regulating members 631 are spaced apart from each other by an interval wider than the thickness of the sample sample part 620.
  • Each of the temperature regulating members 631 includes a first heating region 632, a cooling region 633, a second heating region 634, a third heating region 635, and a light transmitting region 636 in the longitudinal direction of the rectangular plate.
  • the temperature control unit 630 heats and cools the sample sample unit 620 while the temperature control members 631 reciprocate linearly in the horizontal direction.
  • the temperature regulating members 631 heat and cool the sample sample part 620 while reciprocating linearly, the time required for heating and cooling the sample sample part 620 can be reduced. Therefore, the time required for PCR of the sample sample unit 620 can be shortened.
  • the sample sample unit 620 since the sample sample unit 620 is kept in a fixed state, the DNA sample is not shaken to maintain a stable state. Since the shaking of the DNA sample is prevented, the amplification degree of the DNA and the DNA can be accurately determined from the sample sample unit 620.
  • Insulating regions 637 are positioned between the first heating region 632, the cooling region 633, the second heating region 634, the third heating region 635, and the light transmitting region 636.
  • the thermal insulation regions 637 prevent heat from the first heating region 632, the cooling region 633, the second heating region 634, and the third heating region 635 from being transferred to the adjacent region.
  • the insulation regions 637 may be made of an empty space or made of an insulation material.
  • the temperature controller 630 further includes a fixing member 638 and a pair of guide members 639.
  • the fixing member 638 fixes one end portion of the temperature regulating members 631. Therefore, the temperature regulating members 631 can be kept in parallel at a predetermined interval.
  • Guide members 639 are provided at the other end portion opposite to the one end portion of the temperature regulating members 631. Specifically, the guide members 639 may be provided at the other end of the upper surface of the upper temperature regulating member 631 and the other end of the lower surface of the lower temperature regulating member 631.
  • the guide members 639 fix the first heating zone 632, the cooling zone 633, the second heating zone 634, the third heating zone 635, the light transmitting zone 636, and the thermal insulation zone 637. It plays a role.
  • the guide members 639 may protrude in a horizontal direction from the other end portion of the temperature regulating members 631. Since the spacing between the guide members 639 is substantially the same as the thickness of the guide protrusion 614, the protruding guide members 639 may be supported by the guide protrusion 614 of the housing 610. Therefore, even when the temperature regulating members 631 move reciprocally linearly, the guide members 639 are supported by the guide protrusions 614, so that the distance between the temperature regulating members 631 can be kept constant. Therefore, the gap between the temperature regulating members 631 can be narrowed to prevent the collision with the sample sample part 620.
  • the temperature control unit 630 may be directly connected to the wire for supplying external power. Since the temperature controller 630 linearly reciprocates, the wire is not twisted even when the wire is directly connected to the temperature controller 630.
  • the sensor module 640 is provided on the inner upper surface of the housing 610, and determines the DNA amplification degree and the DNA by using light.
  • the sensor module 640 may be fixed to an inner side surface of the housing 610.
  • the sensor module 640 irradiates light onto the sample sample unit 620 while the light transmitting region 636 of the temperature controller 630 is positioned above and below the sample sample unit 620, and the sample sample unit 620. It can receive light transmitted from it.
  • the sensor module 640 may include a light emitting part and a light receiving part, and the light emitting part and the light receiving part may be disposed above and below the sample sample part 620, respectively.
  • the sensor module 640 may include an integrated light emitting unit and a light receiving unit, and the integrated light emitting unit and the light receiving unit may be disposed above or below the sample sample unit 620.
  • the driving unit 660 is provided inside the housing 610, and includes a first heating region 632, a cooling region 633, a second heating region 634, a third heating region 635, and a light transmitting region 636. ) Linearly reciprocates the temperature control unit 630 in a horizontal direction so that) passes sequentially through the sample sample unit 620. Examples of the driving unit 660 include a linear motor, a cylinder, and the like.
  • the temperature controller 630 may include only one of the pair of temperature regulating members 631.
  • the sensor module 640 may not be provided on the inner upper surface of the housing 610, but may be provided in the light transmitting region 636 of the temperature control member 631.
  • exothermic paste composition for forming a thick film according to an embodiment of the present invention includes carbon nanotube particles, carbon nanoparticles, a mixed binder, an organic solvent and a dispersant.
  • the carbon nanotube particles may be selected from single-walled carbon nanotubes, double-walled carbon nanotubes, multi-walled carbon nanotubes, or mixtures thereof.
  • the carbon nanotube particles may be multi wall carbon nanotubes.
  • the diameter may be 5 nm to 30 nm, and the length may be 3 ⁇ m to 40 ⁇ m.
  • the carbon nanoparticles may be, for example, graphite nanoparticles, and the diameter may be 1 ⁇ m to 25 ⁇ m.
  • the mixed binder serves to make the exothermic paste composition have heat resistance even in the temperature range of about 300 ° C., and includes epoxy acrylate or hexamethylene diisocyanate, polyvinyl acetal, and the like.
  • Phenolic resin has a mixed form.
  • the mixed binder may be a mixture of epoxy acrylate, polyvinyl acetal, and phenolic resin, or may be a mixture of hexamethylene diisocyanate, polyvinyl acetal, and phenolic resin.
  • by increasing the heat resistance of the mixed binder even if the heat generated at a high temperature of about 300 °C has the advantage that there is no change in resistance of the material or breakage of the coating film.
  • the phenolic resin means a phenolic compound including phenol and phenol derivatives.
  • the phenol derivative may include p-cresol, o-Guaiacol, Creosol, catechol, 3-methoxy-1,2-benzenediol (3 -methoxy-1,2-Benzenediol), Homocatechol, Vinylguaiacol, Syringol, Iso-eugenol, Methoxyeugenol, o O-Cresol, 3-methyl-1,2-benzenediol, (z) -2-methoxy-4- (1-propenyl) -phenol ( (z) -2-methoxy-4- (1-propenyl) -Phenol), 2, .6-diethoxy-4- (2-propenyl) -phenol (2,6-dimethoxy-4- (2-propenyl) ) -Phenol), 3,4-dimethoxy-Phenol, 4-ethyl-1,3-benzened
  • the mixing ratio of the mixed binder may be a ratio of 10 to 150 parts by weight of polyvinyl acetal resin and 100 to 500 parts by weight of phenolic resin based on 100 parts by weight of epoxy acrylate or hexamethylene diisocyanate. If the content of the phenolic resin is 100 parts by weight or less, the heat resistance characteristics of the heat-paste composition is lowered, and if it exceeds 500 parts by weight, there is a problem that the flexibility is lowered (brittleness increase).
  • the organic solvent is used to disperse the conductive particles and the mixed binder, carbitol acetate, butyl carbotol acetate, dibasic ester, ethyl carbitol, ethyl carbitol acetate, dipropylene It may be a mixed solvent of two or more selected from glycol methyl ether, cellosolve acetate, butyl cellosolve acetate, butanol and octanol.
  • the dispersion process can be applied to a variety of commonly used methods, for example through the ultra-sonication (Roll mill), bead mill (Bead mill) or ball mill (Ball mill) process Can be done.
  • Roll mill ultra-sonication
  • Bead mill bead mill
  • Ball mill ball mill
  • the dispersant is to make the dispersion more smoothly, and a conventional dispersant used in the art such as BYK, an amphoteric surfactant such as Triton X-100, SDS and the like and a ionic surfactant may be used.
  • a conventional dispersant used in the art such as BYK
  • an amphoteric surfactant such as Triton X-100, SDS and the like
  • a ionic surfactant may be used.
  • the heating paste composition according to an embodiment of the present invention may further include 0.5 to 5 parts by weight of the silane coupling agent based on 100 parts by weight of the heating paste composition.
  • the silane coupling agent functions as an adhesion promoter to promote adhesion between the resins in the formulation of the exothermic paste composition.
  • the silane coupling agent may be an epoxy containing silane or a merceto containing silane.
  • Examples of such silane coupling agents include epoxy and include 2- (3,4 epoxy cyclohexyl) -ethyltrimethoxysilane, 3-glycidoxytrimethoxysilane, 3-glycidoxypropyltriethoxysilane, 3-glycidoxypropyltriethoxysilane, containing amine groups, N-2 (aminoethyl) 3-amitopropylmethyldimethoxysilane, N-2 (aminoethyl) 3-aminopropyltrimethoxysilane , N-2 (aminoethyl) 3-aminopropyltrimethoxysilane, 3-aminopropyltriethoxysilane, 3-aminopropy
  • the present invention further provides a planar heating element which is formed by screen-printing, gravure printing (or roll-to-roll gravure printing) or comma coating (or roll-to-roll comma coating) on a substrate of the heating paste composition according to the embodiments of the present invention described above. .
  • the substrate is polycarbonate, polyethylene terephthalate (PET), polyethylene naphthalate (PEN), polyimide, cellulose ester, nylon, polypropylene, polyacrylolintril, polysulfone, polyester sulfone, polyvinylidene fluoride , Glass, glass fiber (matte), ceramic, SUS, copper or aluminum substrate, etc. may be used, but is not limited to those listed above.
  • the substrate may be appropriately selected depending on the application field of the heating element or the use temperature.
  • the planar heating element prints the heating paste composition according to the embodiments of the present invention on the substrate in a desired pattern through screen printing or gravure printing, and after drying and curing, printing and drying / curing the silver paste or the conductive paste on the top.
  • the heat generating paste composition according to the embodiments of the present invention may be formed by screen printing or gravure printing.
  • the surface heating element may further include a protective layer coated on the upper surface.
  • the protective layer may be formed of silica (SiO2).
  • SiO2 silica
  • the heating element has an advantage of maintaining flexibility even if coated on the heating surface.
  • test examples are only examples for explaining the present invention, and the present invention is not limited by the following test examples.
  • Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 CNT particles 4 5 6 4 5 6 CNP Particles 8 9 15 - - - Mixed binder 20 15 22 - - - Ethyl cellulose - - - 10 12 14 Organic solvent 63 67 52 82 79 76 Dispersant (BYK) 5 4 5 4 4 4 4
  • CNT particles and CNP particles (Examples 1 to 3) were added to a carbitol acetate solvent according to the composition of [Table 1], and BYK dispersant was added, and then dispersion A was prepared by sonication for 60 minutes. It was. Thereafter, a mixed binder was added to the carbitol acetate solvent and then a master batch was prepared through mechanical stirring. Next, the dispersion A and the masterbatch were first kneaded through mechanical stirring, followed by a second kneading process through a 3-roll-mill process to prepare an exothermic paste composition.
  • CNT particles were added to the carbitol acetate solvent according to the composition of [Table 1], BYK dispersant was added, and a dispersion was prepared by sonication for 60 minutes. Thereafter, ethyl cellulose was added to the carbitol acetate solvent to prepare a master batch through mechanical stirring. Next, the dispersion B and the masterbatch were first kneaded through mechanical stirring, followed by a second kneading process through a 3-roll mill to prepare an exothermic paste composition.
  • 11 is an image of a planar heating element specimen prepared using the heating paste composition according to the present invention.
  • 11A is a planar heating element formed by screen printing a heat generating paste composition on a polyimide substrate.
  • 11B is a planar heating element formed by screen printing a heating paste composition on a glass fiber mat.
  • 11C and 11D are images when the protective layer is coated on the planar heating element of FIG. 11A (FIG. 11C is a black protective layer coating, and FIG. 11D is a green protective layer coating).
  • planar heating element sample Example
  • planar heating element samples prepared according to the comparative example as shown in FIG. 11A were measured (the applied voltage / current is shown in [Table 2]).
  • the planar heating element corresponding to the above embodiments and comparative examples was heated up to 40 ° C, 100 ° C and 200 ° C, respectively, and the DC voltage when the temperature was reached and The current was measured.
  • Figure 12 shows the image of the heat stability test appearance of the planar heating element samples prepared according to the Examples and Comparative Examples, the test results are summarized in the following [Table 2].
  • Example 1 Example 2 Example 3 Comparative Example 1 Comparative Example 2 Comparative Example 3 Specific resistance ( ⁇ 10 ⁇ 2 ⁇ cm 1.9 2.55 2.96 9.73 8.52 6.23 40 °C reach DC drive voltage / current 5V / 0.2A 6V / 0.2A 7V / 0.2A 20V / 0.3A 16V / 0.2A 12V / 0.2A 100 °C reach DC driving voltage / current 9V / 0.5A 12V / 0.4A 14 V / 0.5 A 48V / 0.7A 40V / 0.7A 26V / 0.6A 200 °C reach DC drive voltage / current 20V / 0.6A 24V / 0.7A 24V / 1.0A - - - Heat stability (day) 20 days or more 20 days or more 20 days or more Bad Bad Bad Bad
  • the specific resistance was measured that the planar heating element corresponding to the embodiments is smaller than the planar heating element corresponding to the comparative examples, accordingly driving voltage / current required to reach each temperature also corresponds to the embodiments
  • the planar heating element was measured smaller than the planar heating element corresponding to the comparative examples. That is, it was confirmed that the planar heating elements corresponding to the embodiments can be driven at a lower voltage and lower power than the comparative example.
  • planar heating elements according to Examples 1 to 3 the stability was maintained for 20 days even under the heating operation of 200 ° C. (no separate protective layer). Poor phenomena were observed to swell the surface of the heating portion within time. That is, it was confirmed that the planar heating element corresponding to the embodiments can be stably driven even at a high temperature of 200 ° C. or more than the comparative example.
  • the present invention further provides a portable heating heater including the planar heating element and a power supply unit for supplying power to the planar heating element.
  • the power supply unit may include a lead electrode coated on the left and right sides of the planar heating element, and a power connection electrode attached to the lead electrode.
  • the power connection electrode may be directly connected to the planar heating element.
  • the lead electrode or the electrode for power connection can be formed using silver paste, copper paste, copper tape, or the like.
  • the portable heating heater according to the present invention has a form in which the planar heating element is attached, embedded or mounted on the inner or outer surface of the body, and has a power supply for driving the planar heating element.
  • the portable heating heater may be used for an inner seat for a baby carriage, a heating sock, a heating shoe, a heating hat, a portable heating mat, a portable cooking utensil, a vehicle heating sheet, and the like.
  • planar heating element employed in the portable heating heater according to the present invention can be driven as a secondary battery capable of charging and discharging, such as a lithium ion battery, a lithium polymer battery because it can be driven at a low voltage and low power as described above, The portability is enhanced and the use time can be greatly increased.
  • the PCR device can heat and cool the sample sample unit while moving the temperature control unit while the sample sample unit is fixed.
  • the efficiency of the PCR device may be improved by reducing the heating and cooling time of the sample sample unit.
  • the DNA detection accuracy of the PCR device since the sample sample portion is maintained in a fixed state, the DNA detection accuracy of the PCR device may be improved, and the heat resistance paste composition may be driven at low voltage and low power due to high heat resistance, small resistance change according to temperature, and low specific resistance. It may include.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Selon un mode de réalisation de la présente invention, un dispositif de PCR comprend : une unité d'échantillonnage d'échantillons qui comporte le matériel nécessaire à la synthèse de l'ADN et est montée fixe ; et une unité d'ajustement de la température située de façon à être adjacente à l'unité d'échantillonnage d'échantillons, comporte au moins un ou plusieurs éléments chauffants de type feuille formés par une composition de pâte chauffante, et se déplace par rapport à l'unité d'échantillonnage d'échantillons qui est montée fixe de façon à ajuster la température de l'unité d'échantillonnage d'échantillons à la température requise à chaque étape de la synthèse de l'ADN, la composition de pâte chauffante comprenant, pour 100 parties en poids de composition de pâte chauffante, de 3 à 6 parties en poids de particules de nanotubes de carbone, de 0,5 à 30 parties en poids de nanoparticules de carbone, de 10 à 30 parties en poids de liant mixte, de 29 à 83 parties en poids de solvant organique, et de 0,5 à 5 parties en poids de dispersant, le liant mixte étant à base d'époxy acrylate, de polyacétal de vinyle, et de résine phénolique en mélange, ou de diisocyanate d'hexaméthylène, de polyacétal de vinyle, et de résine phénolique en mélange.
PCT/KR2016/003286 2015-05-19 2016-03-30 Dispositif de pcr WO2016186312A1 (fr)

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KR10-2015-0069497 2015-05-19

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090048575A (ko) * 2006-06-27 2009-05-14 앤에이오에스 주식회사 카본극세사를 이용한 면상발열체 제조방법 및 이를 통해 제조된 카본극세사를 이용한 면상발열체
KR101123351B1 (ko) * 2008-10-09 2012-03-23 주식회사 엑사이엔씨 고전도성 페이스트 조성물 및 이의 제조방법
JP2013085530A (ja) * 2011-10-20 2013-05-13 Sony Corp 加熱反応用マイクロチップ、加熱反応用マイクロチップの製造方法及び加熱制御方法
KR101294596B1 (ko) * 2012-02-09 2013-08-09 한화케미칼 주식회사 탄소나노튜브를 포함하는 면상 발열체 페이스트 조성물 및 그 제조방법
KR20140128671A (ko) * 2013-04-29 2014-11-06 (주)미코엠에스티 Pcr 장치

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20090048575A (ko) * 2006-06-27 2009-05-14 앤에이오에스 주식회사 카본극세사를 이용한 면상발열체 제조방법 및 이를 통해 제조된 카본극세사를 이용한 면상발열체
KR101123351B1 (ko) * 2008-10-09 2012-03-23 주식회사 엑사이엔씨 고전도성 페이스트 조성물 및 이의 제조방법
JP2013085530A (ja) * 2011-10-20 2013-05-13 Sony Corp 加熱反応用マイクロチップ、加熱反応用マイクロチップの製造方法及び加熱制御方法
KR101294596B1 (ko) * 2012-02-09 2013-08-09 한화케미칼 주식회사 탄소나노튜브를 포함하는 면상 발열체 페이스트 조성물 및 그 제조방법
KR20140128671A (ko) * 2013-04-29 2014-11-06 (주)미코엠에스티 Pcr 장치

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