WO2016183789A1 - 燕窝萃取组成物及含有所述燕窝萃取组成物的食品 - Google Patents

燕窝萃取组成物及含有所述燕窝萃取组成物的食品 Download PDF

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WO2016183789A1
WO2016183789A1 PCT/CN2015/079239 CN2015079239W WO2016183789A1 WO 2016183789 A1 WO2016183789 A1 WO 2016183789A1 CN 2015079239 W CN2015079239 W CN 2015079239W WO 2016183789 A1 WO2016183789 A1 WO 2016183789A1
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Prior art keywords
bird
nest
nest extract
protein
extract composition
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PCT/CN2015/079239
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English (en)
French (fr)
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陈天煌
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陈天煌
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Priority to PCT/CN2015/079239 priority Critical patent/WO2016183789A1/zh
Publication of WO2016183789A1 publication Critical patent/WO2016183789A1/zh

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G3/00—Sweetmeats; Confectionery; Marzipan; Coated or filled products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/06—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from blood
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/24—Mucus; Mucous glands; Bursa; Synovial fluid; Arthral fluid; Excreta; Spinal fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin

Definitions

  • the present invention relates to an extract composition, and more particularly to a composition obtained from bird's nest extraction.
  • Bird's nest is one of the traditional and precious foods that China has been eating since ancient times. It refers to the saliva secreted by several swiftlets of the Swift and the swiftlet genus, and mixed with other substances (such as feathers). Built nests, not nests built by birds of the genus Yankee (such as Jiayan).
  • the protein purity and nutritional value of the swiftlet saliva are the highest. Produced in the southern coastal areas of China, Vietnam, Thailand, Malaysia, Indonesia and the Philippines. After the bird's nest is picked, it must be processed by steaming, soaking, removing impurities, picking, drying and other complex processing to make the finished bird's nest, including swallow, swallow, swallow, and swallow. Among them, the swallow is the whole high-quality bird's nest, which retains the original shape, so the price is particularly expensive.
  • bird's nest acid which is also called sialic acid.
  • Gunnar Blix et al. isolated Sialic acid from submandibular gland mucin in 1952 and established the naming rules for sialic acid, but little is known about its physiological functions for a long time. Its chemical structure was determined exactly in 1960. With the rapid development of modern physics, chemistry and life sciences, the physiological activity of sialic acid has gradually been recognized.
  • Sialic acid is a generic term for a class of derivatives in which amino acids or hydroxy hydrogens are substituted in neuraminic acid, and usually also refers to N-acetylneuraminic acid, one of the most important members of such compounds.
  • N-acetylneuraminic acid (Neu5Ac), N-glycolylneuraminic acid (Neu5Gc) and N-acetyl-9-O-acetylneuraminic acid are most common in the sialic acid family. Among them, Neu5Gc is commonly found in animal cells, but only a small amount appears in tumor cells in humans, while normal cells do not have Neu5Gc.
  • sialic acid In addition to its structural changes, sialic acid also changes with the modification of its own molecules. In addition to this, the manner in which sialic acid and sialic acid are bonded to other sugars also produces a diversity of sialic acid bonding structures.
  • Neu5Ac binds to galactose in an ⁇ 2,3-linked or ⁇ 2,6-linked manner to form Neu5Ac- ⁇ 2,3-Gal and Neu5Ac- ⁇ 2,6-Gal. In human tissues and cells, only Neu5Ac is present, but there is no Neu5Gc.
  • sialic acid is an important component of the structure and function of brain gangliosides and glycoproteins. Because sialic acid is a nutrient of nerve cell membrane and synapse, sialic acid can promote the differentiation, development and nerve regeneration of nerve cells, participate in synaptic transmission, maintain the normal function of cells, and participate in memory learning. Animal experiments have shown that supplementation with sialic acid can improve the learning behavior of animals, and supplementation with sialic acid can significantly increase the content of sialic acid in gangliosides and glycoproteins in the brain and cerebellum, which can improve the test results of experimental animals in maze test. . This result indicates that sialic acid is quite important for learning ability.
  • breastfed babies are able to take large amounts of sialic acid from breast milk, which may promote sialylation of gangliosides and glycoproteins in body fluids, tissues and glycosides, while further showing breastfeeding.
  • the development of the nervous system and intelligence of infants is superior to that of milk powder.
  • breast milk is supplemented with sialic acid for the baby.
  • An effective route because about 0.3-1.5 mg of sialic acid is contained per mL of breast milk.
  • bird's nest contains a glycoprotein that enhances the mitosis of mononuclear cells in human blood by concanavalin A (Con A) or phytohemagglutinin A (PHA).
  • Mitogenic stimulation factor It is like epidermic growth factor, which can stimulate cell division and proliferation and promote cell differentiation, so that damaged epithelial cells can be repaired quickly.
  • This glycoprotein and concanavalin A priming effect had the strongest effect in the first 10 hours.
  • its glycoprotein activity is very stable and does not disappear due to the action of trypsin or extraction with ether in an alkaline environment.
  • sialic acid has the effect of scavenging free radicals in cells, thereby producing an antioxidant effect and thus having an anti-aging effect.
  • an object of the present invention is to provide a bird's nest extract composition and
  • the food having the bird's nest extract composition has a considerable amount of sialic acid and protein.
  • the protein comprises at least one of an epidermal growth factor (EGF) or a fibroblast growth factor (FGF).
  • EGF epidermal growth factor
  • FGF fibroblast growth factor
  • the protein has a molecular weight distribution from 100 Da to 300 kDa.
  • the protein of less than 6.5 kDa comprises from 0.6% to 6.4% by weight of the total protein content.
  • the bird's nest extract is in a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis at 145.991 m/z, 172.000 m/z, 190.07 m/z, 211.987 m/ z, 294.06 m/z, 335.104 m/z, 379.132 m/z, 496.354 m/z, and 1563.790 m/z have characteristic peaks.
  • MALDI-TOF MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • the bird's nest extract is in a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis pattern at 22.701 m/z, 38.666 m/z, 71.715 m/z, 145.875 m/ z, 171.966 m/z, 189.971 m/z, 211.976 m/z, 233.971 m/z, 249.949 m/z, 379.067 m/z, 445.031 m/z, and 655.995 m/z have characteristic peaks.
  • MALDI-TOF MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • the present invention provides a bird's nest extract composition
  • a bird's nest extract composition comprising: a bird's nest extract comprising 1% to 13% by weight of sialic acid and 4% to 15% by weight of protein, wherein the molecular weight distribution of the protein is 100 Da to 300 kDa, and wherein less than 6.5 kDa of protein accounts for 0.6% to 6.4% by weight of the total protein content.
  • the bird's nest extract composition is used to make shampoo, hair lotion, mask, make-up remover, eye cream, hand cream, lotion, bath essence, beauty lotion, body cream, toothpaste, sputum At least one of a cream, a soft candy or a soap.
  • a first preferred embodiment of the present invention is a bird's nest extract composition
  • a bird's nest extract composition comprising a bird's nest extract obtained by a vacuum decompression extraction step, the bird's nest extract further comprising 1% to 13% by weight of sialic acid and 4% to 15% Percentage of protein by weight.
  • the above numerical range of 1% to 13% by weight of sialic acid means that any value between "1%” and "13%” is included, for example, 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, and any positive integer, rational number, and irrational number between the foregoing values, and encompasses any combination of the foregoing various values. range.
  • the bird's nest extract may be further decomposed by protease of at least one of papain, tryptone, or pineapple enzyme during the extraction step.
  • the bird's nest extract composition of the present embodiment may further comprise an excipient in addition to the bird's nest extract, the excipient comprising at least one of starch, corn starch, maltodextrin, lactose, dextrin, or cellulose.
  • the bird's nest extract composition of the present embodiment is suitable for making various solid or semi-solid products such as capsules, emulsions and the like.
  • the extracted bird's nest extract composition contains EGF (Epidermal Growth Factor) or fibroblast growth factor (FGF, Fibroblast). Growth Factor) At least one of the proteins, and the molecular weight distribution of the protein is from 100 Da to 300 kDa. In addition, proteins having a protein content of less than 6.5 kDa are present in an amount of from 0.6% to 6.4% by weight of the total protein.
  • EGF Epidermal Growth Factor
  • FGF fibroblast growth factor
  • the bird's nest extract obtained through the above extraction step may be in the form of powder and is in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis map at 145.991 m/z, 172.000 m/z. 190.017 m/z, 211.987 m/z, 294.06 m/z, 335.104 m/z, 379.132 m/z, 496.354 m/z, and 1563.790 m/z have characteristic peaks.
  • MALDI-TOF MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • the bird's nest extract When the bird's nest extract is liquid, it is in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis map at 22.701m/z, 38.666m/z, 71.715m/z, 145.875m/z, 171.966 m/z, 189.971 m/z, 211.976 m/z, 233.971 m/z, 249.949 m/z, 379.067 m/z, 445.031 m/z, and 655.995 m/z have characteristic peaks. Detailed data and experimental methods are described later.
  • the bird's nest extract composition of the present embodiment can be used to prepare shampoo, hair lotion, facial mask, make-up remover, eye cream, hand cream, lotion, bath essence, beauty liquid, body cream, toothpaste, ⁇ At least one of a cream, a soft candy, or a soap.
  • the present invention further provides a second preferred embodiment, which is also a bird's nest extract composition comprising a bird's nest extract, the bird's nest extract comprising 1% to 13% by weight of sialic acid and 4% to 15% by weight
  • the protein wherein the protein has a molecular weight distribution of from 100 Da to 300 kDa, and wherein the protein of less than 6.5 kDa comprises from 0.6% to 6.4% by weight of the total protein content.
  • the protein contained in the bird's nest extract composition of the present embodiment comprises at least an epidermal growth factor (EGF) or a fibroblast growth factor (FGF). one.
  • the bird's nest extract can be powdery and in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis map at 145.991m/z, 172.000m/z, 190.017m/z, 211.987m/z 294.06 m/z, 335.104 m/z, 379.132 m/z, and 496.354 m/z have characteristic peaks.
  • MALDI-TOF MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • the bird's nest extract When the bird's nest extract is liquid, it is in the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis map at 22.701m/z, 38.666m/z, 71.715m/z, 145.875m/z, 171.966m/z, 189.971m/z, 211.976m/z, 233.971m/z, 249.949m/z, 379.067m/z, 445.031m/z, and 655.995 m/z has a characteristic peak.
  • MALDI-TOF MS matrix-assisted laser desorption ionization time-of-flight mass spectrometry
  • the bird's nest extract composition of the present embodiment is also the same as the previous embodiment, and can be used to prepare shampoo, hair lotion, facial mask, make-up remover, eye cream, hand cream, lotion, bath essence, beauty liquid, body At least one of cream, toothpaste, ointment, fondant, or soap.
  • the bird's nest extract composition of the present invention and the food containing the bird's nest extract composition can achieve anti-oxidation, enhance immunity, enhance memory, or have a considerable amount of sialic acid and protein at the same time. Prevents colds and other effects, and can be used as shampoo, hair lotion, mask, make-up remover, eye cream, hand cream, lotion, bath essence, beauty lotion, body cream, toothpaste, ointment, soft candy, Or products such as soap.
  • bird's nest extract a liquid bird's nest extract
  • the above-mentioned bird's nest extract is mixed with 0 to 10% by weight of an excipient (for example, starch, corn starch, maltodextrin, lactose, dextrin, or cellulose) for lyophilization to obtain a solid (or powder) Bird's nest extract (hereinafter referred to as bird's nest extract powder).
  • an excipient for example, starch, corn starch, maltodextrin, lactose, dextrin, or cellulose
  • the bird's nest extract and the bird's nest extract powder obtained in the above experimental example 1 were subjected to high performance liquid chromatography (Hua Yongyou et al., Chinese Journal of Health Inspection, October 2010, Vol. 20, No. 10, pp. 2454-2456)
  • the sialic acid content of each sample was measured.
  • the results showed that the different samples prepared under the extraction conditions of Experimental Example 1 were repeatedly tested, and the percentage of sialic acid was 1% to 13% (depending on the sample, the bird's nest extract and the bird's nest extract powder may contain 1%). , 2.07%, 3.09%, 8.61%, 10.08%, 13% sialic acid).
  • the references listed above are all incorporated by reference in their entirety.
  • the bird's nest extract and the bird's nest extract powder obtained in the first experimental example contain a considerable amount of sialic acid, and therefore, those skilled in the art to which the present invention pertains, according to the disclosure of the present specification, can be known.
  • the provided bird's nest extract composition and the food containing the bird's nest extract composition can have the functions of anti-oxidation, enhancing immunity, enhancing memory and preventing cold.
  • the bird's nest extract obtained in the above experimental example 1 was further analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to analyze the molecular weight distribution of each molecule in the sample, which was found in the analysis spectrum at 22.701 m/z.
  • MALDI-TOF MS matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
  • the "m/z" (mass-to-charge ratio) unit shown by the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOFMS) is a unit of Dalton (Da) equivalent to the molecular weight. That is, the above-mentioned matrix-assisted laser desorption ionization time-of-flight mass spectrometry method is equivalent to the molecular weight value of "Da" after subtracting 1 from the "m/z" value in the spectrum, for example, The map shows 200 m/z, which is equivalent to a molecular weight of 199 Da.
  • both the bird's nest extract and the bird's nest extract powder contain oligo-peptide molecules (having a molecular weight of less than 1000 Da), which are particularly helpful for percutaneous absorption of organisms (eg, humans) or Absorbed through the digestive tract.
  • the bird's nest extract and the bird's nest extract powder obtained in the above experimental example 1 were subjected to commercial EGF and FGF kits (manufacturer name: Peprotech, and the box names were: Human EGF Mini ELISA Development Kit PEP-900-M05, Human FGF-basic ELISA Development Kit 900-K0) EGF and FGF in samples were detected by sandwich enzyme-linked immunosorbent assay (Sandwich ELISA).
  • Each sample was diluted 40-fold and subjected to three replicates, while the EGF standard was serially diluted twice from 1000 pg/mL (pg/mL) to 15.625 pg/mL (pg/mL), while the FGF standard was from 4000 Pico/ml (pg/mL) was serially diluted twice to 62.5 pg/ml (pg/mL).
  • the capture antibody concentration was 100 ⁇ g/ml ( ⁇ g/mL)
  • the detection antibody concentration was 100 ⁇ g/ml ( ⁇ g/mL).
  • each milliliter of bird's nest extract contained at least 0.04 ⁇ g of EGF, and each gram of bird's nest extract contained at least 0.0832 ⁇ g of EGF, and was tested with bird's nest food (ie, swallow) purchased from other sources.
  • the gram contains less than 31.26 pg of EGF.
  • Each milliliter of bird's nest extract contains at least 0.16 ⁇ g of FGF, and each gram of bird's nest extract contains 38.835 to 261.88 ng of FGF, and is tested with bird's nest food (ie, swallow) purchased from other sources.
  • the gram contains 228.67 to 300.172 pg of FGF.
  • the bird's nest extract and the bird's nest extract powder obtained in the above Experimental Example 1 do contain a considerable amount of EGF and FGF protein, so the bird's nest extract composition provided by the present invention and the bird's nest extract composition containing the same Foods can have the function of promoting epidermal growth, skin care, hair care or wound repair.
  • the bird's nest extract composition obtained in Experimental Example 1 was added with an appropriate amount of water, glycerin, aloe vera extract (Kidachi Aloe Ekisu) and isopropyl myristate, glycerol stearin at a weight percentage of 0.02% to 3%.
  • Acid Glyceryl Stearate
  • cetyl alcohol sorbitol
  • ceteth-15 sorbitan stearate
  • sorbitan stearate Sorbitan Stearate
  • the bird's nest extract composition obtained in Experimental Example 1 was added with 65.7%-93.0% maltodextrin in a weight percentage of 2%-8.5%, and an appropriate amount (400-1000 mg) was packaged in a gelatin capsule to obtain a bird's nest extract composition.
  • the bird's nest extract composition obtained in Experimental Example 1 was added with an appropriate amount of water, lauryl sulfate, lauryl polyoxyethylene sulfate, sodium cocoamphoacetate, coconut at a weight percentage of 0.02% to 3%.
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, a surfactant (for example, sodium lauryl sulfate, sodium laureth sulfate) (Sodium Laureth) in a weight percentage of 0.02% to 3%.
  • a surfactant for example, sodium lauryl sulfate, sodium laureth sulfate) (Sodium Laureth) in a weight percentage of 0.02% to 3%.
  • Sulfate sodium lauryl sulfate, sodium laureth sulfate) (Sodium Laureth)
  • cocamide DEA Cocamide DEA
  • Glycol stearate emollients
  • emulsifiers eg olive oil, polysorbate, sodium chloride ( Sodium chloride)
  • a humectant such as Tocopheryl Acetate E
  • other suitable preservatives such as sodium hydroxymethylglycine
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, Cyclopentasiloxane, emollient (for example, Isohexadecane), perfume, surface at a weight percentage of 0.02% to 3%.
  • Active agents such as Hexylene Glycol, Sodium chloride
  • emulsifiers such as cyclohexasiloxane
  • preservatives such as benzyl alcohol, Phenoxyethanol
  • a fragrance such as sodium citrate
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, a humectant (for example, glycerin, Saccharide isomerate), and a silicon-based smoothing agent (for example, cyclopolymerized) in a weight percentage of 0.02% to 3%.
  • a humectant for example, glycerin, Saccharide isomerate
  • a silicon-based smoothing agent for example, cyclopolymerized
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water and a humectant (for example, Dipropylene glycol, Butylene Glycol, glycerin), alcohol, in an amount of 0.02% to 3% by weight.
  • Emulsifiers such as Methyl gluceth-10, Polyglyceryl-2 diisostearate
  • surfactants eg PEG-60 hydrogenated castor oil (PEG-60 Hydrogenated) Castor Oil
  • Isostearic Acid eg PEG-60 hydrogenated castor oil (PEG-60 Hydrogenated) Castor Oil
  • a emollient such as Triethylhexanoin
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, a humectant (for example, glycerin, propylene glycol), and a surfactant (for example, polysorbate 20) in a weight percentage of 0.02% to 3%. ), PEG-150 distearate (Hydrogenated Castor Oil), emulsifier (such as glyceryl oleate), preservative (such as methyl isothiazolinone ( Methylisothiazolinone)), a suitable fragrance to make a lotion containing bird's nest extract.
  • a humectant for example, glycerin, propylene glycol
  • a surfactant for example, polysorbate 20
  • PEG-150 distearate Hydrogenated Castor Oil
  • emulsifier such as glyceryl oleate
  • preservative such as methyl isothiazolinone ( Methylisothi
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, alcohol, a humectant (for example, glycerin, butylene glycol), an emulsifier (for example, hydrogenated lecithin) at a weight percentage of 0.02% to 3%. Hydrogenated lecithin), a emollient (such as acetyl glutamine (Acetyl Glutamine)), a suitable fragrance, and a preservative (such as Methylparaben) to produce a cosmetic liquid containing bird's nest extract.
  • a humectant for example, glycerin, butylene glycol
  • an emulsifier for example, hydrogenated lecithin
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, a humectant (for example, glycerin, propylene glycol, 1,2-pentanediol (Pentylene Glycol)) at a weight percentage of 0.02% to 3%, suitably Perfumes, emollients (such as Cyclopentasiloxane, Glyceryl Stearate), surfactants (such as PEG-60 Hydrogenated Castor Oil, antioxidants (such as citric acid) Sodium), an emulsifier (such as behenic acid), a preservative, and a suitable fragrance to prepare a body cream containing bird's nest extract.
  • a humectant for example, glycerin, propylene glycol, 1,2-pentanediol (Pentylene Glycol)
  • emollients such as Cyclopentasiloxane, Glyceryl Stearate
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, silicate, muscovite, fluoride, and a surfactant (for example, sodium dodecyl sulfate (SLS)) in a weight percentage of 0.02% to 3%.
  • a surfactant for example, sodium dodecyl sulfate (SLS)
  • SLS sodium dodecyl sulfate
  • a suitable flavor, glycerin, and sorbitol are used to prepare a toothpaste containing a bird's nest extract.
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, honey, rock sugar, and plants in a weight percentage of 0.02% to 3% (for example, eucalyptus leaves, ginseng, platycodon, rehmannia, raw land, Ophiopogon japonicus, Angelica, lily , licorice, menthol, almond) extract to prepare a cream containing bird's nest extract.
  • a weight percentage of 0.02% to 3% for example, eucalyptus leaves, ginseng, platycodon, rehmannia, raw land, Ophiopogon japonicus, Angelica, lily , licorice, menthol, almond
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, oil (for example, palm oil), sodium stearate, appropriate flavors and dyes in a weight percentage of 0.02% to 3% to obtain a bird's nest extract. soap bar.
  • oil for example, palm oil
  • sodium stearate for example, sodium stearate
  • appropriate flavors and dyes in a weight percentage of 0.02% to 3% to obtain a bird's nest extract. soap bar.
  • the bird's nest extract composition obtained in Experimental Example 1 is added with an appropriate amount of water, gelatin, glucose, sucrose, citric acid, and a suitable flavor and sweetener in a weight percentage of 0.02% to 3% to obtain a bird's nest extract.
  • Soft candy

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Abstract

一种燕窝萃取组成物，包含燕窝萃取物，该燕窝萃取物经真空减压萃取步骤所得，该燕窝萃取物包含1％至13％重量百分比的唾液酸与4％至15％重量百分比的蛋白质。还提供一种含有上述燕窝萃取组成物的食品。

Description

燕窝萃取组成物及含有所述燕窝萃取组成物的食品 技术领域
本发明关于一种萃取组成物，特别关于一种自燕窝萃取而得的组成物。
背景技术
燕窝是中国自古代以来开始被食用的传统名贵食品之一，是指雨燕目雨燕科部分雨燕和金丝燕属的几种金丝燕分泌出来的唾液，再混合其它物质(如：羽毛)所筑成的巢穴，而非雀形目燕科鸟类(如家燕)所筑巢穴。
其中以金丝燕唾液的蛋白质纯度和营养价值为最高。产自中国南部沿海一带、越南、泰国、马来西亚、印度尼西亚及菲律宾等地。燕窝被采摘之后，还要经过蒸细、浸泡、除杂、挑毛、烘干等复杂的加工才能制成燕窝成品，包括燕盏、燕条、燕饼、燕丝。其中燕盏是整只优质的燕窝经除杂保留原有形状而成，故价格特别昂贵。
而在燕窝的组成中，含有一种名为燕窝酸的活性物质，其又称为唾液酸。贡纳尔·布利克斯等人于1952年从颌下腺黏蛋白中分离出唾液酸(Sialic acid)，并建立了唾液酸的命名规则，但在很长时期内人们对其生理功能了解甚少，其化学结构在1960年才被确切地测定出来。随着现代物理学、化学和生命科学技术的飞速发展，唾液酸的生理活性已逐渐被认识。唾液酸是神经氨酸中胺基酸或羟基氢被取代的一类衍生物的总称，通常也指代此类化合物中最重要的成员之一N-乙酰神经氨酸。唾液酸在自然界分布很广，目前已知的食物中唾液酸含量最高的是存在燕窝中，含量达12％左右，在牛奶、鸡蛋和奶酪中也存在唾液酸，其主要作为糖蛋白和神经节苷脂的组分。唾液酸取代基通常为乙酰基或羟乙酰基，不过甲基、硫酸酯基、磷酸酯基取代氨基的唾液 酸也已被发现。目前被发现的唾液酸单糖家族约有50种，都是神经胺酸的衍生物，每一种都携带着各种不同的取代基，有的是胺基(amine groups)、有的则是羟基(hydroxyl groups)。
而在唾液酸家族中又以N-乙酰神经氨酸(Neu5Ac)、N-羟乙酰神经氨酸(Neu5Gc)和N-乙酰基-9-O-乙酰神经氨酸最为常见。其中Neu5Gc普遍出现于动物细胞，但在人体中却只有少量出现于肿瘤细胞上，正常的细胞则无Neu5Gc存在。
唾液酸除了本身结构上的变化，也会随着本身分子修饰的不同而有所改变。除此之外，唾液酸与唾液酸之间或与其它的糖的键结方式，也产生唾液酸键结结构的多样性。像是Neu5Ac以α2，3-连结或是α2，6-连结的方式与半乳糖结合，形成Neu5Ac-α2，3-Gal和Neu5Ac-α2，6-Gal。而人类的组织和细胞中只有Neu5Ac，却没有Neu5Gc的存在。也有文献显示，流感病毒的感染与唾液酸有关，因此其中一类的抗流感药物(克流感(oseltamivir)和瑞乐沙(zanamivir))，为模拟唾液酸的类似物，可与病毒的神经胺酸酶也就是唾液酸酶反应，使得病毒无法正常的从宿主细胞中释出，因此无法再感染其它正常细胞。
有文献显示，唾液酸是大脑神经节苷脂和糖蛋白的结构和功能上的重要组成部分。因唾液酸是神经细胞膜及突触的营养素，因此唾液酸可促进神经细胞的分化、发育和神经再生作用，参与突触传递、维持细胞的正常机能，参与记忆学习功能。动物实验研究表明，补充唾液酸可以提高动物的学习行为，而补充唾液酸可以明显增加大脑和小脑的神经节苷脂和糖蛋白中唾液酸的含量，可以提高实验动物在迷宫测试中的测试结果。此结果表明，唾液酸对于学习能力相当重要。也有研究显示，大脑的生长和成熟与唾液酸所参与形成的酸性糖脂与蛋白含量的增加有关，而衰老和先天性发育迟缓综合征与其含量下降有关。通过动物实验发现，在没有唾液酸或唾液酸含量很低的情况下，实验动物的学习及记忆能力均大大下降。
许多研究都证明，母乳喂养的婴儿能够从母乳中摄取大量的唾液酸，其有可能促进神经节苷脂和在体液、组织和配糖体中的糖蛋白的唾液酸化，同时进一步显示出母乳喂养对婴儿的神经系统和智力的发育要优于奶粉喂养。而在婴儿诞生后，母乳则是为婴儿补充唾液酸的 有效途径，因为每mL母乳中含有约0.3-1.5mg的唾液酸。
更进一步地，有研究发现，燕窝含有一种糖蛋白，可增强刀豆蛋白A(Con A)或植物血凝素A(PHA)刺激人体血液中单核细胞的有丝分裂，因而称为「促细胞分裂刺激因子」(mitogenic stimulation factor)。其如同表皮生长因子(epidermic growth factor)能够刺激细胞分裂增殖和促进细胞分化，使得受损的上皮细胞得以快速修复。而此糖蛋白与刀豆蛋白A激发作用在最初的10小时保有最强的效果。除此之外，其糖蛋白的活性非常稳定，并不会因胰蛋白酶的作用或是在碱性环境下经由乙醚萃取而消失。
而在燕窝糖蛋白的寡糖研究方面，有文献显示燕窝糖蛋白上有着各式各样的含唾液酸的O-连接寡糖。而若以酶切下燕窝糖蛋白上N-连接寡糖进行分析，发现燕窝糖蛋白上唾液酸N-连接寡糖含有多量的L-岩藻糖(L-fucose)，而其含的唾液酸是多价的，并且在对抗疾病上可能扮演着重要的角色。另外也有研究指出，经胰酶水解的燕窝可以达到抗流感病毒的效果，可能和燕窝中所含的唾液酸有关。
此外，另有研究文献指出，唾液酸具有清除细胞内自由基的功效，故会产生抗氧化的效果，并且亦因此具有抗老化的功效。
然而，为了方便消费者食用燕窝，市场上有许多即食燕窝产品，多以液体形式出现在市场上，但常有鱼目混珠的现象。随着市民对燕窝及其相关的产品的需求不断增加，反之天然燕窝的供应因过度采摘而严重不足。商人研究开发新的燕窝资源，例如以草燕作代替品，但其功效存疑。燕屋的发明对解决目前燕窝供求矛盾，提供某种程度的缓冲，但是由于市场对天然燕窝的需求有增无减，最后造成市场上充斥着不含唾液酸或是唾液酸含量极低的燕窝伪品或是低质量燕窝，而其是否真有效用仍无法得知。
因此，如何提供一种具有相当效用(例如：抗氧化、提升免疫力、增强记忆力、预防感冒功能)的燕窝萃取组成物，已成为重要课题之一。
发明内容
有鉴于上述课题，本发明的目的为提供一种燕窝萃取组成物及含 有所述燕窝萃取组成物的食品，同时具有相当含量的唾液酸及蛋白质。
为达上述目的，依据本发明的一种燕窝萃取组成物，包含燕窝萃取物，所述燕窝萃取物经真空减压萃取步骤所得，所述燕窝萃取物进一步包含有1％至13％重量百分比的唾液酸与4％至15％重量百分比的蛋白质。
在一实施例中，蛋白质包含有表皮生长因子(EGF，Epidermal Growth Factor)、或纤维母细胞生长因子(FGF，Fibroblast Growth Factor)至少其中之一。
在一实施例中，所述蛋白质的分子量分布为100Da至300kDa。
在一实施例中，小于6.5kDa的蛋白质占总蛋白质含量为0.6％～6.4％重量百分比。
在一实施例中，所述燕窝萃取物于基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)分析图谱中于145.991m/z、172.000m/z、190.017m/z、211.987m/z、294.06m/z、335.104m/z、379.132m/z、496.354m/z以及1563.790m/z具有特征峰。
在一实施例中，所述燕窝萃取物于基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)分析图谱中于22.701m/z、38.666m/z、71.715m/z、145.875m/z、171.966m/z、189.971m/z、211.976m/z、233.971m/z、249.949m/z、379.067m/z、445.031m/z、655.995m/z具有特征峰。
此外，本发明还提供一种燕窝萃取组成物，包含：燕窝萃取物，包含有1％至13％重量百分比的唾液酸以及4％至15％重量百分比的蛋白质，其中所述蛋白质的分子量分布为100Da至300kDa，且其中小于6.5kDa的蛋白质占总蛋白质含量为0.6％～6.4％重量百分比。
在一实施例中，燕窝萃取组成物用以制成洗发精、护发乳、面膜、卸妆液、眼霜、护手霜、化妆水、沐浴精、美容液、身体用乳霜、牙膏、枇杷膏、软糖或是肥皂中至少一种。
此外，本发明还提供一种食品，含有本发明各实施例所述的燕窝萃取组成物。
在一实施例中，所述食品具有抗氧化、提升免疫力、增强记忆力、预防感冒功能中至少一种。
承上所述，本发明的燕窝萃取组成物及含有所述燕窝萃取组成物 的食品，因同时具有相当含量的唾液酸及蛋白质，故具有提升免疫力、增强记忆力、或是预防感冒等功能，而可制成洗发精、护发乳、面膜、卸妆液、眼霜、护手霜、化妆水、沐浴精、美容液、身体用乳霜、牙膏、枇杷膏、软糖、或是肥皂等产品。
具体实施方式
以下将参照相关附图，说明本发明较佳实施例的一种燕窝萃取组成物以及包含此燕窝萃取组成物的食品，其中相同的组件将以相同的参照符号加以说明。此外，以下各实施例及各实验例中所提及的数值范围，如有特别指示及说明外，均包含各数值范围端点值及所述端点值之间的任意数值，且还涵盖任何正整数、有理数及无理数，以及包含前述各种数值的任意组合所涵盖的范围。
本发明第一较佳实施例为一种燕窝萃取组成物，包含经真空减压萃取步骤所得燕窝萃取物，燕窝萃取物进一步包含有1％至13％重量百分比的唾液酸以及4％至15％重量百分比的蛋白质。前述1％至13％重量百分比的唾液酸的数值范围指「1％」及「13％」之间的任意数值均涵盖在内，例如1％、2％、3％、4％、5％、6％、7％、8％、9％、10％、11％、12％、13％以及前述各数值之间的任何正整数、有理数及无理数，并且包含前述各种数值的任意组合所涵盖的范围。前述4％至15％重量百分比的蛋白质的数值范围，同样地，指「4％」及「15％」之间的任意数值均涵盖在内，且亦涵盖任何正整数、有理数及无理数，例如4％、5％、6％、7％、8％、9％、10％、11％、12％、13％、14％、15％，以及包含前述各种数值的任意组合所涵盖的范围。
而本实施例在进行萃取步骤时，燕窝萃取物进一步可经由木瓜酶、胰蛋白脢、或菠萝酶至少其中之一的蛋白酶分解所得。且本实施例的燕窝萃取组成物，除燕窝萃取物之外，可进一步包含有赋形剂，赋形剂包含有淀粉、玉米淀粉、麦芽糊精、乳糖、糊精、或纤维素中至少一种，使本实施例的燕窝萃取组成物适于制成各种固态或半固态的产品，例如胶囊、乳液等。
而萃取所得的燕窝萃取组成物，包含有表皮生长因子(EGF，Epidermal Growth Factor)、或纤维母细胞生长因子(FGF，Fibroblast  Growth Factor)至少其中之一种蛋白质，且蛋白质的分子量分布为100Da至300kDa。另外，小于6.5kDa的蛋白质占总蛋白质含量为0.6％～6.4％重量百分比。
进一步来说，经由上述萃取步骤所得的燕窝萃取物，可呈粉状，且于基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)分析图谱中于145.991m/z、172.000m/z、190.017m/z、211.987m/z、294.06m/z、335.104m/z、379.132m/z、496.354m/z以及1563.790m/z具有特征峰。而当燕窝萃取物为液状时，于基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)分析图谱中于22.701m/z、38.666m/z、71.715m/z、145.875m/z、171.966m/z、189.971m/z、211.976m/z、233.971m/z、249.949m/z、379.067m/z、445.031m/z、655.995m/z具有特征峰。详细数据及实验方式如后所述。
而本实施例的燕窝萃取组成物，可用以制成洗发精、护发乳、面膜、卸妆液、眼霜、护手霜、化妆水、沐浴精、美容液、身体用乳霜、牙膏、枇杷膏、软糖、或是肥皂中至少一种。
上述各项产品的添加成分，如后实验例六中所述。
另外，本发明进一步提供第二较佳实施例，也为一种燕窝萃取组成物，包含燕窝萃取物，燕窝萃取物包含有1％至13％重量百分比的唾液酸以及4％至15％重量百分比的蛋白质，其中蛋白质的分子量分布为100Da至300kDa，且其中小于6.5kDa的蛋白质占总蛋白质含量为0.6％～6.4％重量百分比。
与前述较佳实施例类似，本实施例的燕窝萃取组成物中所含的蛋白质，包含有表皮生长因子(EGF，Epidermal Growth Factor)、或纤维母细胞生长因子(FGF，Fibroblast Growth Factor)至少其中之一。且燕窝萃取物可为粉状，并于基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)分析图谱中于145.991m/z、172.000m/z、190.017m/z、211.987m/z、294.06m/z、335.104m/z、379.132m/z、以及496.354m/z具有特征峰。而当燕窝萃取物为液状时，于基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)分析图谱中于22.701m/z、38.666m/z、71.715m/z、145.875m/z、171.966m/z、189.971m/z、211.976m/z、233.971m/z、249.949m/z、379.067m/z、445.031m/z、以及655.995 m/z具有特征峰。
本实施例的燕窝萃取组成物，也与前实施例相同，可用以制成洗发精、护发乳、面膜、卸妆液、眼霜、护手霜、化妆水、沐浴精、美容液、身体用乳霜、牙膏、枇杷膏、软糖、或是肥皂中至少一种。
另外，本发明进一步提供第三较佳实施例，为一种包含有前述第一或是第二较佳实施例所述的燕窝萃取组成物的食品。
而本较佳实施例的食品，经由下述实验数据显示，具有提升免疫力、增强记忆力、或是预防感冒等功能。
综上所述，本发明的一种燕窝萃取组成物以及包含此燕窝萃取组成物的食品，故可因同时具有相当含量的唾液酸及蛋白质，达到抗氧化、提升免疫力、增强记忆力、或是预防感冒等功效，进而可制成洗发精、护发乳、面膜、卸妆液、眼霜、护手霜、化妆水、沐浴精、美容液、身体用乳霜、牙膏、枇杷膏、软糖、或是肥皂等产品。以下以各实验例及相关实验数据证实其具有相当含量的唾液酸、寡肽与表皮生长因子(EGF，Epidermal Growth Factor)及纤维母细胞生长因子(FGF，Fibroblast Growth Factor)等蛋白质，故可具有促进表皮生长、皮肤保养、护发、伤口修补、抗氧化、提升免疫力、增强记忆力、或预防感冒等效果。
实验例一：燕窝萃取组成物的萃取
首先，取天然燕窝磨碎后的燕窝粉末6公斤，加入114公升的无菌蒸馏水中，并进行真空减压萃取，亦即于摄氏30～50度环境中抽取真空(压力为10～200托(torr))维持30分钟至12小时。待冷却至摄氏37度之后，加入228公克的蛋白酶(可为木瓜酶、胰蛋白脢、或菠萝酶)，维持于摄氏37度下作用1～12小时，之后以加热方式中止酶反应。
接着过滤上述经过蛋白酶反应完毕后的液体，以滤除未溶解的杂质，并将滤液减压浓缩后以巴式低温杀菌法进行杀菌，取得液态的燕窝萃取物(以下简称燕窝萃取液)。将前述燕窝萃取液混入0～10％重量百分比的赋形剂(例如：淀粉、玉米淀粉、麦芽糊精、乳糖、糊精、或纤维素)进行冻干处理，以取得固态(或是粉状)的燕窝萃取物(以下简称燕窝萃取粉)。
实验例二：燕窝萃取物中粗蛋白质含量的分析
将上述实验例一中取得的燕窝萃取液以及燕窝萃取粉，以经济部中央标准局食品中粗蛋白质的检验法(CNS总号5035，类号N6116，又俗称「凯氏法」(Kjeldahl method))分别进行粗蛋白质含量的测定。以实验例一中萃取条件下所制得的不同样本进行重复测验，结果显示每100公克的燕窝萃取液以及燕窝萃取粉中含有4至15公克的粗蛋白质(依不同样本，每100公克的燕窝萃取液以及燕窝萃取粉中可含有4、4.9、11.57、14.6、15公克的粗蛋白质)。
实验例三：燕窝萃取物中唾液酸含量分析
将上述实验例一中取得的燕窝萃取液以及燕窝萃取粉，以高效液相色谱法(华永有等人，中国卫生检验杂志，2010年10月第20卷第10期，页2454-2456)检测各样本的唾液酸含量。结果显示，以实验例一的萃取条件下所制得的不同样本进行重复测验，其唾液酸的重量百分比为1％至13％(依不同样本，燕窝萃取液以及燕窝萃取粉中可含有1％、2.07％、3.09％、8.61％、10.08％、13％的唾液酸)。而上述所列的参考文献全体皆引用作为本说明书的公开内容。
经由上述实验结果可知，实验例一中取得的燕窝萃取液以及燕窝萃取粉含有相当含量的唾液酸，因此本发明所属技术领域具有通常知识者，依据本说明书的公开内容，当可得知本发明所提供的燕窝萃取组成物及含有此种燕窝萃取组成物的食品可具有抗氧化、提升免疫力、增强记忆力、预防感冒的功能。
实验例四：燕窝萃取物中蛋白质分子量分布分析
将上述实验例一中取得的燕窝萃取液以及燕窝萃取粉，以十二烷基硫酸钠聚丙烯酰胺凝胶电泳(sodium dodecyl sulfate polyacrylamide gel electrophoresis，SDS-PAGE)来分析样本中蛋白质的分子量分布。前述十二烷基硫酸钠聚丙烯酰胺凝胶浓度为12％及15％，且添加有10％重量百分比的甘油(glycerol)，并将实验后的胶体以Bio-rad gel DOC XR+系统影像分析装置扫描及分析计算各样本中的不同分子量的蛋白质含量。以实验例一的萃取条件下所制得的不同样本进行重复测验，结果显示，经实验例一所制得的燕窝萃取液以及燕窝萃取粉中，小于6.5kDa的蛋白质占总蛋白质含量约是0.6％至6.4％重量百分比， 以其中一样本进行前述分析的蛋白质分子量分布分析结果如下表一所示。
表一：燕窝萃取液以及燕窝萃取粉的蛋白质分布
  燕窝萃取粉 燕窝萃取液
分子量(kDa) 重量百分比(％) 重量百分比(％)
＞100 7.9-15.3 1.4-1.6
100-75 11.6-20.6 1.4-4.4
75-50 14.1-35.1 22.9-27.5
50-35 9.4-12.6 2.9-25.9
35-25 6.4-16.6 4.5-5.5
25-15 16.8-30 24.2-56
15-6.5 0.7-3.7 4.1-10.7
＜6.5 0.6-1.5 0.6-6.4
此外，将上述实验例一中取得的燕窝萃取粉，经由基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)进一步分析样本中各分子的分子量分布，发现在分析图谱中于145.991m/z、172.000m/z、190.017m/z、211.987m/z、294.065m/z、335.104m/z、379.132m/z、496.354m/z、1563.790m/z、5312.775m/z、6220.710m/z、5419.701m/z、7802.584m/z、5562.115m/z、24966.772m/z、以及5916.553m/z处有特征峰(peak)。
而将上述实验例一中取得的燕窝萃取液，经由基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOF MS)进一步分析样本中各分子的分子量分布，发现在分析图谱中于22.701m/z、38.666m/z、71.715m/z、145.875m/z、171.966m/z、189.971m/z、211.976m/z、233.971m/z、249.949m/z、379.067m/z、445.031m/z、655.995m/z、5308.455m/z、5417.040m/z、5553.870m/z、5865.525m/z、6219.320m/z、7593.220m/z、7824.713m/z、8147.532m/z、以及25756.060m/z处有特征峰(peak)。
上述基质辅助激光解吸电离飞行时间质谱分析法(MALDI-TOFMS)显示的「m/z」(质荷比)单位，即相当于分子量的道尔顿(Dalton，Da)单位。亦即上述基质辅助激光解吸电离飞行时间质谱分析法于图谱中「m/z」数值减1后即相当于「Da」为单位的分子量数值，例如 图谱中显示200m/z，即是相当于其分子量为199Da。
上述结果显示，不论是燕窝萃取液以及燕窝萃取粉的样本中，均含有寡肽(oligo-peptide)分子(其分子量小于1000Da)，特别有助于生物体(例如：人体)经皮吸收或是经消化道吸收。
实验例五：燕窝萃取物中EGF与FGF检测
将上述实验例一中取得的燕窝萃取液以及燕窝萃取粉，利用商用EGF与FGF试剂盒(厂商名：Peprotech，盒名分别为：Human EGF Mini ELISA Development Kit PEP-900-M05、Human FGF-basic ELISA Development Kit 900-K0)以夹心酶联免疫分析法(sandwich enzyme-linked immunosorbent assay，Sandwich ELISA)检测样本中的EGF及FGF。每样本稀释至40倍，并进行三重复，而EGF标准品自1000皮克/毫升(pg/mL)连续两倍稀释至15.625皮克/毫升(pg/mL)，而FGF标准品则自4000皮克/毫升(pg/mL)连续两倍稀释至62.5皮克/毫升(pg/mL)。其中捕捉抗体(Capture Antibody)浓度为100微克/毫升(μg/mL)，而检测抗体(Detection Antibody)浓度为100微克/毫升(μg/mL)。并以卵白素结合辣根过氧化物酶(Avidin-HRP conjugate)与ABTS(2，2′-连氮基双-(3-乙基苯并噻唑啉-6-磺酸酯))进行呈色反应，每五分钟测定一次于450nm与650nm读取反应的吸光值，并且控制最高浓度的标准品吸光值不可超过1.2，最低浓度的标准品吸光值不可超过0.2。其余实验步骤则依照所利用商用EGF与FGF试剂盒中所附的说明进行操作。
结果显示，每毫升的燕窝萃取液至少含有0.04μg的EGF，而每克的燕窝萃取粉至少含有0.0832μg的EGF，同时以其它来源购得的燕窝食品(即食燕)进行测试，其产品中每克含有的EGF小于31.26pg。而每毫升的燕窝萃取液中至少含有0.16μg的FGF，而每克的燕窝萃取粉含有38.835～261.88ng的FGF，同时以其它来源购得的燕窝食品(即食燕)进行测试，其产品中每克含有228.67～300.172pg的FGF。
由上述实验结果可知，经上述实验例一中取得的燕窝萃取液以及燕窝萃取粉确实含有相当含量的EGF及FGF蛋白质，故本发明所提供的燕窝萃取组成物及含有此种燕窝萃取组成物的食品可具有促进表皮生长、皮肤保养、护发或是伤口修补的功能。
实验例六：燕窝萃取组成物所制得的各产品添加成分
A.护手霜
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、甘油、芦荟精华液(Kidachi Aloe Ekisu)与肉豆蔻酸异丙酯(isopropyl myristate)、甘油硬脂酸(Glyceryl Stearate)、鲸蜡醇(cetyl alcohol)、山梨糖醇(Sorbitol)、鲸蜡醇聚醚-15(Ceteth-15)、失水山梨糖醇硬脂酸酯(Sorbitan Stearate)及其它适当的乳化剂，以制得含有燕窝萃取物的护手霜。
B.燕萃胶囊
以实验例一所取得的燕窝萃取组成物以2％-8.5％的重量百分比添加65.7％-93.0％的麦芽糊精，并取适当量(400-1000毫克)包装于明胶胶囊中，以制得含有燕窝萃取物的燕萃胶囊。
C.洗发精
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、十二烷基硫酸盐、十二烷基聚氧乙烯硫酸盐、椰油基两性醋酸钠、椰油基单乙醇酰胺、二甲聚硅氧烷(Dimethicone)、胶原蛋白、维生素、植物提取物(例如：芦荟精华液)、珠光剂、保湿剂、香精、色素、防腐剂、螯合剂(例如：EDTA)，以制得含有燕窝萃取物的洗发精。
D.护发乳
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、表面活性剂(例如月桂硫酸钠(Sodium Lauryl Sulfate)、月桂醇聚醚硫酸酯钠盐(Sodium Laureth Sulfate)、椰油酰胺DEA(Cocamide DEA)、及/或乙二醇硬脂酸酯(Glycol stearate))、柔润剂、乳化剂(例如橄榄油、聚山梨醇酯(Polysorbate)、氯化钠(Sodium chloride))、保湿剂(例如醋酸盐维他命E(Tocopheryl Acetate E))、及其它适当的防腐剂(例如羟甲基甘氨酸钠)、及香料，以制得含有燕窝萃取物的护发乳。
E.面膜
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的熊果素、玻尿酸、弹性蛋白(Elastin)、海藻萃取物、羟苯 丙酯、水，并涂附于适当基材(例如：不织布、羽丝绒、天丝棉、生物纤维)上，以制得含有燕窝萃取物的面膜。
F.卸妆液
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、环戊硅氧烷(Cyclopentasiloxane)、柔润剂(例如异十六烷(Isohexadecane))、香料、表面活性剂(例如己二醇(Hexylene Glycol)、氯化钠(Sodium chloride))、乳化剂(例如环己硅氧烷(Cyclohexasiloxane))、防腐剂(例如苯甲醇、苯氧乙醇(Phenoxyethanol))、香料、及抗氧化剂(例如柠檬酸钠)，以制得含有燕窝萃取物的卸妆液。
G.眼霜
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、保湿剂(例如甘油、异构寡糖(Saccharide isomerate))，硅基顺滑剂(例如环聚二甲基硅氧烷(Cyclomethicone))、植物油、抗氧化剂(例如维生素E)，以制得含有燕窝萃取物的眼霜。
H.化妆水
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、保湿剂(例如双丙甘醇(Dipropylene glycol、丁二醇(Butylene Glycol)、甘油)、酒精、乳化剂(例如甲基葡糖聚醚-10(Methyl gluceth-10)、聚甘油-2二异硬脂酸(Polyglyceryl-2diisostearate))、表面活性剂(例如PEG-60氢化篦麻油(PEG-60Hydrogenated Castor Oil)、异硬脂酸(Isostearic Acid))、柔润剂(例如三异辛酸甘油酯(Triethylhexanoin))、适当防腐剂，以制得含有燕窝萃取物的化妆水。
I.沐浴精
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、保湿剂(例如甘油、丙烯乙二醇)、表面活性剂(例如聚山梨醇酯20(Polysorbate 20)、PEG-150二硬脂酸酯(PEG 150Distearate)、氢化蓖麻油(Hydrogenated Castor Oil))、乳化剂(例如甘油油酸酯(Glyceryl oleate))、防腐剂(例如甲基异噻唑啉酮(Methylisothiazolinone))、适当香料，以制得含有燕窝萃取物的化妆水。
J.美容液
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、酒精、保湿剂(例如甘油、丁二醇(Butylene Glycol))、乳化剂(例如氢化卵磷脂(Hydrogenated lecithin))、柔润剂(例如乙酰谷氨酰胺(Acetyl Glutamine))、适当香料、防腐剂(例如羟苯甲酸甲脂(Methylparaben))，以制得含有燕窝萃取物的美容液。
K.身体用乳霜
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、保湿剂(例如甘油、丙烯乙二醇、1，2-戊二醇(Pentylene Glycol))、适当香料、柔润剂(例如环戊硅氧烷(Cyclopentasiloxane)、甘油硬脂酸(Glyceryl Stearate))、表面活性剂(例如PEG-60氢化篦麻油(PEG-60Hydrogenated Castor Oil)、抗氧化剂(例如柠檬酸钠)、乳化剂(例如山嵛酸(Behenic acid))、防腐剂、适当香料，以制得含有燕窝萃取物的身体用乳霜。
L.牙膏
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、硅酸盐、白云母、氟化物、表面活性剂(例如十二烷基硫酸钠(SLS))、适当香料、甘油、山梨糖醇，以制得含有燕窝萃取物的牙膏。
M.枇杷膏
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、蜂蜜、冰糖、植物(例如枇杷叶、元参、桔梗、熟地、生地、麦冬、当归、百合、甘草、薄荷脑、杏仁)萃取物，以制得含有燕窝萃取物的枇杷膏。
N.肥皂
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、油(例如棕榈油)、硬脂酸钠、适当香料和染料，以制得含有燕窝萃取物的肥皂。
O.软糖
以实验例一所取得的燕窝萃取组成物以0.02％-3％的重量百分比添加适量的水、明胶、葡萄糖、蔗糖、柠檬酸、及适当香料与甜味剂，以制得含有燕窝萃取物的软糖。
以上所述仅为举例性，而非为限制性。任何未脱离本发明的精神与范畴，而对其进行的等效修改或变更，均应包含于后附的权利要求中。

Claims (10)

  1. 一种燕窝萃取组成物，包含：
    燕窝萃取物，所述燕窝萃取物经真空减压萃取步骤所得，所述燕窝萃取物进一步包含有：
    1％至13％重量百分比的唾液酸；
    4％至15％重量百分比的蛋白质。
  2. 如权利要求1所述的燕窝萃取组成物，其中所述蛋白质包含有表皮生长因子，或纤维母细胞生长因子至少其中之一。
  3. 如权利要求1所述的燕窝萃取组成物，其中所述蛋白质的分子量分布为100Da至300kDa。
  4. 如权利要求1所述的燕窝萃取组成物，其中小于6.5kDa的蛋白质占总蛋白质含量为0.6％～6.4％重量百分比。
  5. 如权利要求1所述的燕窝萃取组成物，其中所述燕窝萃取物于基质辅助激光解吸电离飞行时间质谱分析法分析图谱中于145.991m/z、172.000m/z、190.017m/z、211.987m/z、294.06m/z、335.104m/z、379.132m/z、以及496.354m/z具有特征峰。
  6. 如权利要求1所述的燕窝萃取组成物，其中所述燕窝萃取物于基质辅助激光解吸电离飞行时间质谱分析法分析图谱中于22.701m/z、38.666m/z、71.715m/z、145.875m/z、171.966m/z、189.971m/z、211.976m/z、233.971m/z、249.949m/z、379.067m/z、445.031m/z、以及655.995m/z具有特征峰。
  7. 一种燕窝萃取组成物，包含：
    燕窝萃取物，包含有：
    1％至13％重量百分比的唾液酸；
    4％至15％重量百分比的蛋白质，其中所述蛋白质的分子量分布为100Da至300kDa，且其中小于6.5kDa的蛋白质占总蛋白质含量为0.6％～6.4％重量百分比。
  8. 如权利要求1至7中任一项所述的燕窝萃取组成物，用以制成洗发精、护发乳、面膜、卸妆液、眼霜、护手霜、化妆水、沐浴精、美容液、身体用乳霜、牙膏、枇杷膏、软糖、或肥皂中至少一种。
  9. 一种食品，含有如权利要求1至7中任一项所述的燕窝萃取组成物。
  10. 如权利要求9所述的食品，其具有抗氧化、提升免疫力、增强记忆力、预防感冒功能中至少一种。
PCT/CN2015/079239 2015-05-19 2015-05-19 燕窝萃取组成物及含有所述燕窝萃取组成物的食品 WO2016183789A1 (zh)

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CN114886116A (zh) * 2022-03-18 2022-08-12 广东丸美生物技术股份有限公司 一种调理育龄女性健康的组合物及其制备方法和饮料及应用
CN114903171A (zh) * 2022-05-24 2022-08-16 闵乐晓 一种玻尿酸美颜燕窝及其制备方法
CN115201347A (zh) * 2021-04-13 2022-10-18 中轻食品检验认证有限公司 一种检测食品中发酵来源唾液酸的方法

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115201347A (zh) * 2021-04-13 2022-10-18 中轻食品检验认证有限公司 一种检测食品中发酵来源唾液酸的方法
CN113229490A (zh) * 2021-04-17 2021-08-10 北京御本堂安国中药材产业研究院有限公司 一种花露燕窝及其制备方法
CN114886116A (zh) * 2022-03-18 2022-08-12 广东丸美生物技术股份有限公司 一种调理育龄女性健康的组合物及其制备方法和饮料及应用
CN114886116B (zh) * 2022-03-18 2023-11-21 广东丸美生物技术股份有限公司 一种调理育龄女性健康的组合物及其制备方法和饮料及应用
CN114903171A (zh) * 2022-05-24 2022-08-16 闵乐晓 一种玻尿酸美颜燕窝及其制备方法

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