WO2016171482A1 - Cosmetic composition including apitoxin extract, and method of manufacturing same - Google Patents

Abstract

The present invention relates to a cosmetic composition using apitoxin and Houttuynia cordata extracts and, more particularly, to a cosmetic composition having a fat soluble extract and a water soluble extract of an apitoxin and Houttuynia cordata extract mixed together so as to show anti-aging effects such as the alleviation of skin wrinkles, and to a method of manufacturing the cosmetic composition. The cosmetic composition of the present invention expedites collagen synthesis and activates the ECM (Epidermal Cell Metabolism) so as to show effects of skin condition improvement including the alleviation of skin wrinkles.

Description

Cosmetic composition comprising bee venom extract and preparation method thereof

The present invention relates to a cosmetic composition using bee venom and eochochocho extract, and more particularly, to a cosmetic composition exhibiting anti-aging effects such as wrinkle improvement by mixing fat soluble extract of bee venom and water soluble extract and echochocho extract and a method for producing the same. will be.

This application claims priority based on Korean Patent Application No. 10-2015-0057894 filed on April 24, 2015, and all the contents disclosed in the specification and drawings of the application are incorporated in this application.

The venom that comes from the bee's spawning tube is called Bee Venom. Bee venom is a natural animal bioactive substance stored in bee abdominal sac and linked to bee sting and secreted at the time of stimulation. It has been recorded that bee venom was used for therapeutic purposes in Egypt and Babylonia. In Korea, records of the use of raw acupuncture for the treatment of pain and rheumatoid arthritis have been recorded in the Demon King's white paper (the first acupuncture literature), consent and folk remedies.

This bee venom reduces edema in inflammatory animals, acts on synovial cells in patients with arthritis, and its main ingredient, melittin, inactivates NF-ΚB, reducing the amount of nitric oxide (NO) and cyclooxygena. It is known to induce anti-inflammatory effects by inhibiting Cyclooxygenase-2 (COX-2). There have been reports of physiological studies confirming the effects of bee venom treatment by separately purifying the components of bee venom and treating them with dementia or cancer. In oriental medicine, it has been used for the treatment of inflammatory diseases for a long time and studies the anti-inflammatory and anticancer effects of bee venom.

Meanwhile, Eoseongcho is a perennial herb belonging to more than three hundred, and its scientific name is Houttuynia. Cordata , and according to the Chinese Medicine Dictionary (Shanghai Science and Technology Publishing House), there are other names such as medicinal wheat and ten.

It is described that Echoseongcho removes boils and poisons along with antipyretic action, and Chinese herbal medicine dictionary clears blood and removes inflammation.

Eoseongcho contains active ingredients throughout the leaves, stems, and roots, and bioactive substances, including their unique odors, help metabolism to clear blood, promote kidney function, and excrete inflammatory symptoms and toxins in the body. Since ancient times, it has been widely used as a folk medicine in China and Japan.

Herbal herbs are used for hypertension, constipation, diabetes, fever, boil, detoxification, arthritis, inflammatory diseases, etc. The main ingredients are decanoyl acetaldehyde, melon and myrsen, which are essential oils in the outpost. Aldehydes have a titer of 40,000 times that of sulfamine antibiotics. In addition, in relation to Eoseongcho extract, Korean Patent Application Publication No. 2014-0124172 and Korean Patent Registration No. 1383519 disclose that the content is applied as one component of a cosmetic composition.

On the other hand, the present inventors have disclosed a process for preparing bee venom extract using a fat-soluble solvent and a water-soluble solvent in Korean Patent No. 1425018, in the manufacturing process when separating the water-soluble solvent layer and the fat-soluble solvent layer to separate from each other In this case, the intermediate layer appears to be difficult to completely separate, which causes a problem of inconsistent yield.

Therefore, the situation for the development of a method for producing a cosmetic composition using bee venom extract is constantly required.

Accordingly, the present inventors research and efforts to provide a cosmetic composition excellent in improving skin wrinkles and a method for producing a cosmetic composition that can ensure a constant yield, as a result, obtain bee venom extract according to filtration using a fat-soluble solvent and a water-soluble solvent, When the mixture with Eochocho extract in a certain ratio, the present invention was completed by discovering that a cosmetic composition excellent in improving skin wrinkles can be prepared according to collagen metabolism activation while securing a constant yield of fat-soluble extract and water-soluble extract.

Therefore, an object of the present invention is to provide a cosmetic composition having excellent skin wrinkle improvement effect by activating collagen metabolism including bee venom extract and a method for producing the same.

The present invention is characterized in that the cosmetic composition comprising 50 to 500 parts by weight of Echochocho extract against 100 parts by weight of the bee venom extract mixed with the fat soluble extract of bee venom extracted by the fat-soluble solvent and the water soluble extract of bee venom extracted by the water-soluble solvent do.

The cosmetic composition of the present invention is characterized in that the fat-soluble extract and the water-soluble extract are mixed in a weight ratio of 1: 0.1-10.

In addition, in the cosmetic composition of the present invention, the fat-soluble solvent is one or two or more mixed solvents selected from hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane and chloroform. The water-soluble solvent is characterized in that one or two or more mixed solvents selected from water and C ₁ ~ C ₄ alcohol.

In addition, in the cosmetic composition of the present invention, the Echoseongcho extract is characterized in that extracted with one or two or more mixed solvents selected from water and C ₁ ~ C ₄ alcohol.

In addition, the cosmetic composition of the present invention is 5 to 40% by weight melittin, 1 to 20% by weight of eicosenol, phospholipase A-2 1 to 20% by weight, 0.1 to 5% by weight of apamin (Apamin), It comprises 0.01 to 5% by weight of pinocembrin and 0.01 to 5% by weight of chrysin, characterized in that it comprises 1 to 10% by weight of quercitrin and 0.01 to 5% by weight of quercetin.

On the other hand, the present invention, the bee venom in the fat-soluble solvent and filtered to obtain a fat-soluble extract of the filtrate;

Putting the residue filtered by the filtration into a water-soluble solvent to obtain a water-soluble extract of the filtrate;

Preparing a bee venom extract by mixing the fat-soluble extract and a water-soluble extract; And

It characterized by another method for producing a cosmetic composition comprising the step of mixing 50 to 500 parts by weight of Eochocho extract based on 100 parts by weight of the bee venom extract.

In the manufacturing method of the cosmetic composition, the bee venom extract is characterized in that the fat-soluble extract and water-soluble extract is prepared by mixing in a weight ratio of 1: 0.1 to 10.

On the other hand, the present invention is another feature of a microneedle patch containing the cosmetic composition.

The cosmetic composition of the present invention promotes collagen synthesis and activates skin cell metabolism (ECM: Epidermal Cell Metabolism), thereby improving skin conditions including skin wrinkles.

In addition, the cosmetic composition of the present invention can be used safely without cytotoxicity and skin side effects.

1 is a schematic diagram showing a manufacturing process of the cosmetic composition according to an embodiment of the present invention.

2 is a result confirming the procollagen synthesis effect and MMP-1 expression inhibitory effect of the cosmetic composition according to an embodiment of the present invention.

3 is a result of the comparative experiment of the cosmetic composition and Adenosine according to an embodiment of the present invention.

4 is a graph comparing collagen metabolic activity (Pro-collagen / MMP-1 ratio) of the cosmetic composition according to an embodiment of the present invention.

5 is a result of a comparative experiment of the cosmetic composition according to an embodiment of the present invention and EGF (Epidermal Growth Factor) and EGCG [(-)-Epigallocatechin-3-O-gallate].

Figure 6 is a result of comparing the collagen synthesis effect and MMP-1 expression inhibitory effect according to the weight ratio of bee venom extract and Echochocho extract in the embodiment of the present invention.

7 is a diagram illustrating a process of preparing a mixed solution for preparing a microneedle patch on which a cosmetic composition according to an embodiment of the present invention is mounted.

8 is an experimental result for confirming the effective concentration range of the composition when applying the microneedle patch with a cosmetic composition according to an embodiment of the present invention.

The present invention is characterized by a cosmetic composition comprising a bee venom extract and Eochocho extract mixed with a fat soluble extract of bee venom extracted with a fat soluble solvent and a water soluble extract of bee venom extracted with a water soluble solvent.

Bee venom is a venom nectar from the bee spawning tube, and the bee venom used in the present invention may be live bee venom, dried bee venom or purified bee venom. Raw bee venom is not dried or purified and has a specific gravity of 1.1 to 1.3, a pH in the range of 5.2 to 5.5, and contains 70 to 80% by weight of the peptide.

The cosmetic composition of the present invention contains 50 to 500 parts by weight of Echochocho extract, and preferably 80 to 400 parts by weight, more preferably 100 to 300 parts by weight, based on 100 parts by weight of the bee venom extract.

In the cosmetic composition of the present invention, the bee venom extract is mixed with the fat soluble extract of bee venom extracted by the fat soluble solvent and the water soluble extract of bee venom extracted by the water soluble solvent, so that the fat soluble component and the water soluble component for skin wrinkle improvement are included in an optimal ratio.

The bee venom extract may be mixed with a fat-soluble extract and a water-soluble extract in a weight ratio of 1: 0.1 to 10, preferably 1: 1: 0.2 to 5.0, a weight ratio of more preferably 1: 0.5 to 2.0 It is more advantageous for the wrinkle improvement effect.

As the fat-soluble solvent, one or two or more mixed solvents selected from hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane and chloroform may be used, preferably ethyl acetate or Preference is given to using methyl acetate.

In addition, one or two or more kinds of mixed solvents selected from water and C ₁ to C ₄ alcohols may be used as the water-soluble solvent, and preferably water, methanol or ethanol is used.

On the other hand, Eochocho extract ( Houtuynia) included in the cosmetic composition of the present invention Cordata ) may be extracted using one or two or more mixed solvents selected from water and C ₁ to C ₄ alcohols, and preferably, extracts using water, methanol or ethanol.

The cosmetic composition of the present invention is 5 to 40% by weight melittin derived from bee venom extract, 1 to 20% by weight of eicosenol, phospholipase A-2 1 to 20% by weight, 0.1 to 5% by weight of apamin (Apamin) %, 0.01-5% by weight of pinocembrin and 0.01-5% by weight of chrysin, preferably 10-30% by weight of melittin, 5-15% by weight of eicosenol %, 5-15 wt% of phospholipase A-2, 0.5-3 wt% of Apamin, 0.01-3 wt% of pinocembrin, and 0.01-3 wt% of chrysin. Can be.

In addition, the cosmetic composition of the present invention may include 1 to 10% by weight of Quercitrin and 0.01 to 5% by weight of Quercetin derived from Eochocho extract, and preferably 2 to 8% by weight of Quercetin, Quercetin or The derivatives (Quercetin-glucose, Quercetin-galactose) may comprise 0.01 to 3% by weight.

Wrinkle development is known to be due to decreased collagen metabolism of the skin, collagen synthesis decreases due to oxidative stress or light damage, and increased degradation activity of Matrix metallo proteinase (MMP). Exposure of blasts to hydrogen peroxide stress decreased the amount of procollagen mRNA and protein and increased the amount of MMP-1 mRNA and protein due to oxidative stress. The cosmetic composition of the present invention inhibits the MMP, promotes collagen synthesis, and ultimately activates skin cell metabolism (ECM: Epidermal Cell Metabolism) to improve skin conditions including skin wrinkles.

In addition, pinocembrin and eicosenol in bee venom extract, together with quercitrin and quercetin in Echo extract, promote collagen synthesis of skin cells and activate tyrosinase. It may have a whitening effect of inhibiting melanin production by participating in inhibition. Quatcitrin is a bioflavonoid that has antioxidant properties and serves to inhibit skin damage and cell death by antibacterial and anti-inflammatory, anti-allergic, oxidation and UV rays.

And the cosmetic composition of the present invention can ensure safety without cytotoxicity and skin side effects.

On the other hand, the present invention, the bee venom in the fat-soluble solvent and filtered to obtain a fat-soluble extract of the filtrate; Putting the residue filtered by the filtration into a water-soluble solvent to obtain a water-soluble extract of the filtrate; Preparing a bee venom extract by mixing the fat-soluble extract and a water-soluble extract; And a method for preparing a cosmetic composition comprising mixing 50 to 500 parts by weight of Eochocho extract based on 100 parts by weight of the bee venom extract.

More specifically, crude raw bee venom was added to one or two or more fat-soluble solvents selected from hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane and chloroform, and stirred and filtered. Get a fat-soluble extract. It is preferable to further proceed with the ultrasonication after the stirring. In addition, the pore diameter when the filtration can be used to filter paper in the range of 0.1 ~ 10 μm, through which the fat-soluble extract and the residue of the filtrate is separated.

After drying the filtered residue by filtration and put into one or two or more water-soluble solvent selected from water and C ₁ ~ C ₄ alcohol can be stirred and filtered to obtain a water-soluble extract of the filtrate. It is preferable to further proceed with the ultrasonication after the stirring. In addition, the pore diameter during the filtration can be used to filter paper in the range 0.1 ~ 10 μm, through which the aqueous extract and insoluble matter of the filtrate is further separated.

After drying the fat-soluble extract and the water-soluble extract, respectively, the fat-soluble extract and the water-soluble extract are mixed in a weight ratio of 1: 0.1 to 10 to prepare bee venom extract.

In addition, the extract of Eoseongcho which is one component of the cosmetic composition of the present invention is prepared by extracting by a conventional method in the art, preferably one or two or more mixed solvents selected from water and C ₁ to C ₄ alcohols. It can be extracted by ultrasonication at room temperature. Next, 50 to 500 parts by weight, preferably 80 to 400 parts by weight, and more preferably 100 to 300 parts by weight of Eochocho extract, based on 100 parts by weight of the bee venom extract, to prepare a cosmetic composition.

The cosmetic composition comprises a cosmetically or dermatologically acceptable medium or base. These are all formulations suitable for topical application, e.g. emulsions obtained by dispersing the oil phase in solutions, gels, solids, pasty anhydrous products, aqueous phases, emulsions obtained by dispersing the aqueous phase in oil phases, multiemulsions, suspensions, micro emulsions, micro It may be provided in the form of an aerosol composition containing capsules, microgranulocytes, ionic (liposomal) and nonionic vesicle dispersants, foams or compressed propellants. These compositions can be prepared according to conventional methods in the art.

Cosmetic compositions may include fatty substances, organic solvents, solubilizers, thickeners, gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants, water, ionic or nonionic emulsifiers, fillers , Metal ion sequestrants, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or auxiliaries commonly used in the cosmetic or dermatological fields. have. Such adjuvants are introduced in amounts generally used in the cosmetic or dermatological fields.

In addition, the cosmetic composition is not particularly limited in the formulation, it is possible to appropriately select the formulation as desired. For example, flexible lotion, astringent lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, eye essence, cleansing cream, cleansing lotion, cleansing foam, cleansing water, pack, powder, conceal stick, body lotion, body Creams, body oils, body essences, body cleansers, hair dyes, hair tonics, hair nourishing lotions, hair essences, hair serums, scalp treatments, hair treatments, hair conditioners, hair shampoos, hair lotions, toothpastes, mouthwashes, hair conditioners , Wool, lotions, ointments, gels, creams, patches, and sprays may be prepared in one or more formulations selected from the group consisting of, but not limited to.

In particular, the cosmetic composition may affect the dermal layer by penetrating the skin barrier through the microneedle patch. The microneedles can be locally and continuously injected with active ingredients and can minimize pain when inserted into the skin. The cosmetic composition may be mixed with a biodegradable polymer having excellent biocompatibility and mounted on a microneedle patch. When the mixed viscous solution is dried, the cosmetic composition may be in a solid state and may be re-dissolved by body fluid when injected into the body. As the biodegradable polymer, hyaluronic acid may be used, but is not limited thereto. In addition, the active ingredient concentration in the cosmetic composition mounted on the microneedle patch may be 0.01 ~ 5.0 μg / ㎖, more preferably 0.1 ~ 3.0 μg / ㎖, most preferably 0.1 ~ 2.0 μg / ㎖.

The present invention will be described in detail through the following examples. However, this is only to aid the understanding of the invention, the present invention should not be considered to be limited thereto.

Example

[Preparation of Bee Venom Extract]

50 g of crude raw bee venom powder was added to a beaker, and 400 ml of ethyl acetate (EA) was added thereto, followed by mixing for 20 minutes with a stirrer. The stirred bee venom was treated with ultrasonic waves (40 KHz, 4.5 A) for 10 minutes, and the solution dissolved in ethyl acetate was filtered through a filter paper having a pore diameter of 1 μm. The fat-soluble extract of the filtrate was separated by the filtration, and the residue on the filtered filter paper was left in a hood (23 ° C.) for 20 hours to dry the solvent. 400 ml of distilled water was added to the dried residue, followed by stirring for 20 minutes to dissolve by sonication for 10 minutes. This solution was filtered through a filter paper with a pore diameter of 1 μm as above to separate the aqueous extract of the filtrate.

The fat-soluble extract and the water-soluble extract were dried and mixed in a weight ratio of 1: 2 to prepare bee venom extract.

[Preparation of Echo Extract]

4L of 99.9% ethanol (Ethanol), which is 20 times the weight, is added to 200 g of dried edible vinegar, and the solution obtained by repeatedly extracting the solution obtained by sonication at room temperature for 30 minutes and then left for 30 minutes is concentrated and dried. Prepared.

[Production of Cosmetic Composition and Component Analysis]

The bee venom extract and Eochocho extract were put in a solvent and mixed at a weight ratio of 1: 2 to obtain a cosmetic composition, and the results of component analysis of the cosmetic composition are shown in Table 1 below.

Ingredient Name	Weight ratio (%)
melittin	27.04 ± 1.20
eicosanol	9.42 ± 0.84
PLA2	9.23 ± 5.23
Apamin	1.01 ± 0.98
Pinocembrin	0.04 ± 0.15
Chrysin	0.04 ± 0.13
Quercitrin	4.36 ± 0.48
Quercetin	0.06 ± 0.026

Experimental Example 1 Procollagen Synthesis and Collagen Inhibitor MMP-1 Expression Inhibitory Effect

After pretreatment with bee venom extract and cosmetic composition of the above Example to fibroblasts (NHDF) and exposed to UV-B (30mJ / cm ² ) and incubated for 24 hours, mRNA was isolated by RT-PCR method to procollagen-I gene And the expression level of the MMP-1 gene was confirmed (Fig. 2).

In FIG. 2, M3 shows the result of treatment of the bee venom extract of Example at a concentration of 0.083 μg / ml, and M4 is a cosmetic product in which the bee venom extract and Echochocho extract of the example were mixed at concentrations of 0.083 μg / ml and 0.167 μg / ml, respectively. It is the result of processing a composition.

As shown in FIG. 2, while inhibiting collagen synthesis of fibroblasts by UV-B, MMP-1 expression was significantly increased. When the bee venom extract and Eochocho extract of the present invention were mixed at a certain concentration, the cosmetic composition significantly increased the activity of collagen metabolism and suppressed MMP-1 expression by UV-B.

In addition, the results of the comparative experiment with the positive control group Adenosine known as wrinkle improvement material having an ECM (extracellular matrix) improvement effect is shown in Figure 3, MMP-1 expression by UV-B is superior to the positive control group It can be confirmed that can be suppressed.

In addition, the Pro-collagen / MMP-1 ratio was calculated and shown in FIG. 4, and as shown in FIG. 4, the bee venom extract itself showed less than the result of the positive control Adenosine treatment (10 μM, 100 μM). , The cosmetic composition of the present invention showed an excellent value compared to the positive control group, it was confirmed that the collagen metabolism activity was significantly increased.

Therefore, it can be seen that the composition of the present invention not only has a protective effect of regulating the metabolism of ECM when exposed to stress caused by UV-B irradiation, but also has an excellent rejuvenation effect that induces the synthesis of new collagen. .

Experimental Example 2 Comparison of Effects with Anti-Aging Components

EGCG [(-)-Epigallocatechin-3-O, an anti-aging active ingredient of green tea, known as an anti-aging functional substance, EGF (Epidermal Growth Factor) and MMP-1 inhibitor, to compare the effects with other anti-aging ingredients -gallate].

For this purpose, EGF 10ppm (10μg / ㎖), EGCG 0.5uM (0.23μg / ㎖) using the M4 composition and the same experiment as in Experiment 1, the results are shown in FIG.

As shown in FIG. 5, the M4 composition of the present invention can be confirmed that the anti-aging effect is superior to EGF or EGCG.

Experimental Example 3 Comparison of Effects of Concentrations of Bee Venom Extract and Estuary

In order to compare the effect of each extract by mixing ratio, the composition was treated with different concentrations of bee venom extract and Echochocho extract as shown in Table 2, and the results are shown in FIG.

No	Bee Venom Extract (μg / mL)	Eochocho extract (μg / mL)
One	0.042	0.167
2	0.042	0.334
3	0.083	0.167
4	0.083	0.334
5	0.167	0.167
6	0.167	0.334

 As shown in FIG. 6, when the Pro-collagen / MMP-1 ratio was considered, the No. 3 sample showed the highest ECM metabolic activation, and an excellent effect when the weight ratio of bee venom extract and Echo extract was about 1: 2. It could be confirmed that indicates.

Experimental Example 4 Administration Effect by Microneedle Patch

The microneedle patch was manufactured so that the composition of the present invention penetrates the skin barrier and affects the dermal layer. The procedure is as follows.

First, viscous solution was prepared by mixing 1.92 g of hyaluronic acid, 3 mL of distilled water, and 0.2 mL of the M4 composition at 4 ° C. with 60% (w / v, dissolved in distilled water).

Next, the viscous solution was dispensed in the form of droplets (droplet) of the mixed solution on one surface of the metal substrate, and dried at room temperature through air blowing while adjusting the length of the microneedle through the tensile process to prepare a microneedle patch. The manufacturing process is shown in FIG.

The final length of the microneedle was set to about 300 μm to enable cosmetic use, and an average length of 332.79 ± 27.80 μm and an average top diameter of 42.01 ± 6.04 μm were prepared. The microneedle-shaped patch was used as a gel foam patch (polyurethane film) attached, prepared in a size of 2 cm X 2 cm, 64 micro needles per patch were mounted at 1 mm intervals.

The microneedle was manufactured by adjusting the sum of concentrations of bee venom extract and Echochocho extract in the M4 composition to be 0.16, 0.25, 0.42, 0.50 μg / ml, and the same experiment as in Experimental Example 1 was conducted, and the results are illustrated in FIG. 8 is shown.

As a result, it was confirmed that the concentration of the M4 composition inhibits MMP-1 expression in a concentration-dependent manner in the range of 0.25 μg / ml or more.

Although the above description has been made with reference to embodiments of the present invention, this is merely exemplary, and a person skilled in the art to which the present invention pertains will understand that various modifications and equivalent other embodiments are possible. . Therefore, the true technical protection scope of the present invention will be determined by the claims described below.

Claims (10)

1. Based on 100 parts by weight of bee venom extract in which fat soluble extract of bee venom extracted by fat soluble solvent and water soluble extract of bee venom extracted by water soluble solvent are mixed,

   Cosmetic composition comprising 50 to 500 parts by weight of Eoseongcho extract.
2. The method of claim 1,

   The fat-soluble extract and the water-soluble extract is a cosmetic composition, characterized in that mixed in a weight ratio of 1: 0.1 to 10.
3. The method of claim 1,

   The fat-soluble solvent is a cosmetic composition, characterized in that one or two or more mixed solvents selected from hexane, ether, glycerol, propylene glycol, butylene glycol, ethyl acetate, methyl acetate, dichloromethane and chloroform.
4. The method of claim 1,

   The water-soluble solvent is a cosmetic composition, characterized in that one or two or more mixed solvents selected from water and C ₁ ~ C ₄ alcohol.
5. The method of claim 1,

   The Echo extract is a cosmetic composition, characterized in that extracted with one or two or more mixed solvents selected from water and C ₁ ~ C ₄ alcohol.
6. The method of claim 1,

   The cosmetic composition is 5 to 40% by weight melittin, 1 to 20% by weight of eicosenol, phospholipase A-2 1 to 20% by weight, 0.1 to 5% by weight of apamin (Apamin), pinosembrine ( pinocembrin) 0.01 to 5% by weight and chrysin (chrysin) cosmetic composition comprising a 0.01 to 5% by weight.
7. The method of claim 1,

   The cosmetic composition is a cosmetic composition comprising 1 to 10% by weight of quercitrin and 0.01 to 5% by weight of quercetin.
8. Putting bee venom in a fat soluble solvent and filtering to obtain a fat soluble extract of the filtrate;

   Putting the residue filtered by the filtration into a water-soluble solvent to obtain a water-soluble extract of the filtrate;

   Preparing a bee venom extract by mixing the fat-soluble extract and a water-soluble extract; And

   Method for producing a cosmetic composition comprising the step of mixing 50 to 500 parts by weight of Eoseongcho extract based on 100 parts by weight of the bee venom extract.
9. The method of claim 8,

   Method for producing a cosmetic composition, characterized in that to prepare a bee venom extract by mixing the fat-soluble extract and water-soluble extract in a weight ratio of 1: 0.1 to 10.
10. A microneedle patch comprising the cosmetic composition of any one of claims 1 to 7.

Images