KR20130108826A - Cosmetic composition for inhibiting aging - Google Patents

Abstract

PURPOSE: A cosmetic composition for suppressing aging is provided to show an antioxidation effect without toxicity and side effects and to improve superior skin moisturizing and anti-wrinkling effects using safe bee venom and royal jelly. CONSTITUTION: A cosmetic composition for suppressing aging contains bee venom which prevents wrinkles and royal jelly which moisturizes skin as active ingredients. The composition contains 10^-3 to 10^-1 wt% of the royal jelly and 10^-10-10^-8 wt% of the bee venom. The composition is used for a diet in a form selected among a powder, a granule, a capsule, and a liquid.

Description

COSMETIC COMPOSITION FOR INHIBITING AGING [0002]

The present invention relates to a cosmetic composition for inhibiting skin aging, and more particularly, to a cosmetic composition for inhibiting skin aging, which contains bee venom and royal jelly as active ingredients and has excellent skin moisturizing ability and wrinkle formation inhibiting ability.

Skin is the primary protective membrane of the human body. It protects the body from changes in temperature and humidity, stimulates the external environment such as ultraviolet rays and pollutants, and plays an important role in keeping the body homeostatic such as body temperature control.

However, if the metabolism of the cells is not properly performed and the cells are inactivated due to various pollutants and stresses, the regeneration and proliferation can not be properly performed, the skin is damaged and the skin ages quickly.

Such skin aging occurs at various sites, and skin symptoms generally appear as dull skin, wrinkles, dry skin, and the like. Therefore, in order to solve this problem, it is necessary to understand various aging mechanisms and try to solve them.

In other words, the biomimetic skin cells increase rapidly as the age of the skin cells reaches the level of peroxide. If the constituents of the cell (or cell membrane) become peroxidized by active oxygen and protein damage occurs, the cell or cell membrane will not be able to function properly.

That is, it is known that wrinkles are caused by their functional and structural damage and that they accelerate aging.

These aging phenomena occur naturally over time and can not be prevented 100%, but various methods can slow or prevent the occurrence of aging.

Currently, these cosmetic products, medicines and foods containing substances having active oxygen inhibition effect are known. However, since they are synthetic, they are not safe because they are not safe when applied to human skin for a long period of time. We are developing materials that are applied to our products through efforts such as fermenting natural ingredients.

However, although they are guaranteed to be safe, most of them have a weak active oxygen inhibition effect, so that there is a problem that the effect of preventing and improving wrinkles and the effect of moisturizing ability are insignificant.

An object of the present invention is to provide a cosmetic composition for inhibiting skin aging, which has excellent cytostatic function and wrinkle formation inhibitory ability by containing bee venom and royal jelly which are safe to human body as cytotoxic and effective ingredient.

It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not intended to limit the invention to the particular embodiments that are described. It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory and are not restrictive of the invention, There will be.

To achieve the above object, the cosmetic composition for inhibiting skin aging according to the present invention is characterized by containing bee venom having an ability to suppress wrinkle formation and royal jelly having skin moisturizing effect as an active ingredient.

The royal jelly is contained in an amount of 10 ^-3 to 10 ^-1 % by weight based on the weight of the composition.

The bee venom is contained in an amount of 10 ^-10 to 10 ^-8 % by weight based on the weight of the composition.

The composition is composed of any one selected from powder, granule, capsule, and liquid, and is used as a food.

The method for producing a cosmetic composition for inhibiting skin aging according to the present invention comprises the steps of dissolving a bee venom isolated from bees in water and centrifuging and then freeze-drying the supernatant to prepare a bee venom dried product, freezing and drying the raw royal jelly, And a step of mixing the dried bee ware and the dried product of royal jelly.

The method of inhibiting skin aging according to the present invention is characterized in that skin moisturizing effect and wrinkle formation inhibiting effect are obtained by ingesting the above-mentioned cosmetic composition.

According to the present invention, there is provided a cosmetic composition for inhibiting skin senescence having excellent skin moisturizing ability and wrinkle formation inhibitory ability by containing bee venom and royal jelly which are antioxidative, toxic,

Also, cosmetic compositions for inhibiting skin aging are provided that can be used by food use and are more conveniently usable by consumers.

BRIEF DESCRIPTION OF THE DRAWINGS FIG. 1 is a graph showing the degree of weight gain of a composition for inhibiting skin aging according to the present invention. FIG.
Fig. 2 is a view showing the skin wrinkle suppressing effect of the composition for suppressing skin aging according to the feeding of the present invention. Fig.
(A) UV +
(B) UV-
(C) Example 3 (BRJ3)
(D) Example 4 (BRJ4)
(E) Example 2 (BRJ2)
(F) Example 1 (BRJ1)
Fig. 3 is a graph showing moisture content of the skin according to the feeding of the composition for inhibiting skin aging according to the present invention. Fig.
4 is a graph showing serum IgE levels according to the dietary supply of the composition for inhibiting skin aging according to the present invention.
5 is a graph showing the antioxidative effect of the composition for inhibiting skin aging according to the feeding of the present invention.

The terms, techniques, and the like described in this specification are used in the meaning commonly used in the technical field to which the present invention belongs, unless otherwise specified.

The present invention is characterized by providing a cosmetic composition for inhibiting skin aging comprising bee venom and royal jelly as active ingredients.

To explain, the bee venom, which is contained in a bee venom sac, consists of about 40 kinds of substances and has been used to treat diseases of the human body since BC.

Currently, studies on the effects and mechanisms of bee venom components, hypersensitivity and toxicity of bee venom, immunotherapy and arthritis, herpes simplex, multiple sclerosis, treatment of tumors, etc. have been reported. Known.

In the present invention, the bee venom is preferably separated from the bee, then dissolved in water and centrifuged to use a bee venom dried product in which the supernatant is freeze-dried.

In addition, the royal jelly is a substance produced by bees eating a large amount of seeds and pollen, and is a nutrient containing protein, fat, carbohydrates and various trace elements.

Royal jelly contains a lot of vitamins and rich nutrients. Especially, the neurotransmitters of acetylcholine, which enriches the skin of women, and the parotin-like substance, which is a salivary gland hormone that ages muscles, bones and teeth, .

In addition, through the royal jelly, the sebaceous glands on the surface of the skin secrete natural oily fractions, so that moisture can be adequately retained, thereby maintaining moisture on the skin surface.

In the present invention, it is preferable that the royal jelly is lyophilized and the dried royal jelly is used.

Thus, according to the present invention, royal jelly having skin moisturizing ability and bee venom having wrinkle formation inhibiting effect are included as active ingredients in comparison with cosmetic composition containing only royal jelly, And the effect portion is further added through bee venom to provide a cosmetic composition having an effect of suppressing the aging of the skin.

That is, the effect of suppressing skin wrinkles is much wrinkled when the ultraviolet rays are irradiated (UV +), whereas the composition for suppressing skin aging according to the present invention containing ultraviolet light (UV +) but including bee venom and royal jelly is fed In particular, the composition containing 0.1 g of bee venom + 10 g of royal jelly exhibits the highest skin wrinkle inhibitory effect. When the skin moisturizing effect is only irradiated with ultraviolet rays (UV +), It was confirmed that the composition for inhibiting skin aging according to the present invention containing bee venom and royal jelly showed a high skin moisturizing effect in the treatment group to which the diet was supplied.

Preferably, the royal jelly is contained in an amount of 10 ^-3 to 10 ^-1 wt% based on the weight of the composition, and the bee venom is contained in an amount of 10 ^-10 to 10 ^-8 wt% based on the weight of the composition .

This is because when the content of the royal jelly and bee venom is less than the above weight percentage, the wrinkle suppressing effect and the moisturizing effect of the desired skin are lowered. If the content exceeds the above weight percentage, the royal jelly and bee venom are rather toxic, ) Is lowered and it is difficult to diet, so that it is manufactured in a state unsuitable for use in food use.

As described above, the present invention can provide a cosmetic composition for inhibiting skin aging with excellent skin moisturizing ability and wrinkle formation inhibiting ability by containing bee venom and royal jelly which are safe and toxic and have little side effects.

The composition may be used in any form. However, in order to obtain a higher skin wrinkle suppressing effect and a moisturizing effect, it is preferable that the composition is composed of any one of powder, granule, capsule or liquid, Higher efficiency can be expected.

The method for producing the composition for inhibiting skin aging according to the present invention will be described in detail as follows.

The cosmetic composition of the present invention may contain other components that can give rise to a synergistic effect of the main effect within a range not impairing the intended main effect of the present invention. For example, a perfume, a pigment, An additive such as an antiseptic, an antioxidant, an antiseptic, a moisturizing agent, a thickening agent, an inorganic salt, an emulsifying agent and a synthetic high molecular substance may be further added and the amount of the additive added may be selected within a range not to impair the objects and effects of the present invention .

The above components may be mixed and selected without difficulty by a person skilled in the art according to the formulation or purpose of use, and it is preferable to use any one of powders, granules, capsules or liquid preparations in order to have excellent skin moisturizing ability and wrinkle formation inhibiting ability Type so that it can be used for food use, so that skin moisturizing effect and wrinkle formation inhibiting effect can be obtained through ingestion, so that skin aging can be suppressed.

Hereinafter, the present invention will be described in detail with reference to Examples and Experimental Examples, but the scope of the present invention is not limited thereto.

Examples 1 to 4 Preparation of a cosmetic composition for inhibiting skin aging according to the present invention

1) Bee Venom

The honey bee ( Apis meliffera L.) was collected by using a bee venom collecting device, and the collected bee venom was dissolved with distilled water, centrifuged, filtered, and lyophilized to separate the pure bee venom.

2) Royal jelly

100 g of raw royal jelly in the form of a jelly, which is a natural state obtained by taking the undiluted solution, was lyophilized and pulverized to prepare a dry jelly in the form of a dry powder.

3) Preparation of cosmetic composition

The bee venom and royal jelly were prepared at the combination ratio shown in Table 1 below, and the following experiments were conducted.

Component unit Treated group BRJ1
(Example 1) BRJ2
(Example 2) BRJ3
(Example 3) BRJ4
(Example 4) Casein
(casein) g / kg 230 230 230 230 El-cystine
(L-cystine) g / kg 3 3 3 3 Corn oil
corn oil, g / kg 100 100 100 100 cellulose
(cellulose) g / kg 50 50 50 50 Vitamin mixture
(vitamin mix) g / kg 10 10 10 10 Mineral mixture
(mineral mix) g / kg 35 35 35 35 Sucrose
(sucrose) g / kg 200 200 200 200 Corn starch
(corn starch) g / kg 367 362 347 367 Tablet beige Mu g / kg 0.01 0.01 0.1 0.1 Royal jelly g / kg 10 100 10 100

<Experimental Example 1> Measurement of body weight gain and dietary efficiency according to the feeding of the composition for inhibiting skin aging of the present invention

1. Experimental animals

Male SKH-1 hairless mice of about 20 g were used

2. Experimental conditions

1) Dietary condition

The animals were adapted to the solid diet for 1 week before feeding and divided into 6 groups according to body weight. The experimental group was fed with experimental diet for 6 weeks in parallel with ultraviolet irradiation.

2) Dietary composition

As shown in the above Table 1, the composition containing BRJ1, purified bee venom 0.01 g / kg and royal jelly 100 g / kg is hereinafter referred to as BRJ2, purified bee venom 0.1 g / kg and 10 g / kg of royal jelly were labeled as BRJ3, and the composition containing 0.1 g / kg of tablet bee venom and 100 g / kg of royal jelly as BRJ4.

3) Dietary supplement group

For each 1.0% of the total dietary weight, each of the royal jelly solids was mixed in the diet. The difference in dry weight of the royal jelly samples was adjusted by subtracting the corn starch.

4) UV irradiation

Using UVB (containing about 30% UVA), the animals were irradiated with 2MED (200 mJ / cm ² ) once a day for the first week, and 3 times a week from 2 to 6 weeks Wrinkles were induced by irradiating 4 MED (400 mJ / cm ² ) to the back of the animal.

In the following, ultraviolet rays were indicated as UV + and unexposed groups were indicated as UV-.

3. Analysis method

To measure dietary efficiency, the weight, remaining dietary, dietary, and body weight of each mouse were measured twice a week.

4. Experimental results

The results of the experiment are shown in Table 2 and FIG.

Average diet intake (g / mice / day) 1 week 2 weeks 3 weeks 4 weeks 5 weeks 6 weeks UV- 3.1 2.9 2.8 2.8 2.8 2.8 UV + 3.6 3.4 3.2 3.3 3.2 3.4 BRJ1 3.4 3.3 3.3 3.5 3.2 3.5 BRJ2 3.8 3.4 3.4 3.6 3.4 3.5 BRJ3 3.5 3.3 3.2 3.3 3.3 3.5 BRJ4 3.5 3.5 3.5 3.5 3.4 3.6

As shown in FIG. 1, the initial weight of each test group divided by the nodule method was about 21-22 g, and the body weight of all groups including the UV-irradiated control group increased during the experimental period, There was no significant difference in body weights between 26.1g (UV-group), 26.4g (UV + group), 27.3g (BRJ1), 27.4g (BRJ2), 26.3g (BRJ3) and 26.1g

In addition, as shown in Table 2, the dietary intake for 6 weeks in all groups including UV-irradiated control group ranged from 2.8 to 3.8 (g / mice / day).

There was no statistically significant difference in food efficiency rate (weight gain (g) / dietary intake (g)) between 0.01 and 0.12 in each group.

<Experimental Example 2> Measurement of skin wrinkle inhibitory effect of the composition for suppressing skin aging according to the feeding of the present invention

1. Experimental animals and conditions

The same procedure as in Experimental Example 1 was carried out.

2. Experimental method

1) Manufacture and analysis of skin molds

The flask was placed on the back of a hairless mouse, and a silicone mixture prepared by adding about 1.5 g of light bodied silicone and one drop of a catalyst and a thinner was poured into a flask.

After it was confirmed to be completely dry, the mold was carefully removed from the skin and stored in a dry place.

The incident angle of the light was fixed at 20 °, and the generated shadow was photographed with a CCD camera to generate an image file. At this time, the amount of wrinkle shadows and darkness was converted into the amount of wrinkles using a skin viscometer (SV 600).

2) Animal sacrifice

To investigate the efficacy of aging-induced hairless mice on skin wrinkles, skin tissue was sampled after sacrificing cervical dislocation at 6 weeks of dietary supplementation.

Skin tissues and blood were sampled as described above for the following moisturizing and antioxidant effects.

3. Experimental Results

As shown in FIG. 2, as shown in FIG. 2, it was confirmed that large vertical wrinkles were increased in the UV + group (B) irradiated with ultraviolet light as compared with a normal control group (A: UV-group) supplied with normal diet for 6 weeks without ultraviolet irradiation.

On the contrary, it was found that the treatment groups (C, D, E, and F) after the 6-week feeding of the composition of the present invention combined with ultraviolet irradiation suppressed the formation of large wrinkles in comparison with the UV + group (B).

&Lt; Experimental Example 3 > The effect of the composition for inhibiting skin aging of the present invention on moisturizing skin moisture

1. Experimental animals and conditions

The same procedure as in Experimental Example 1 was carried out.

2. Experimental method

In the same manner as in Experimental Example 2, a mixture of HBSS (Hank's Balanced Salt Solution) and Dispase in a ratio of 1: 1 was added to the skin of the sacrificed animal in order to confirm the moisturizing effect of both the epidermis and the dermis The skin was treated at 4 캜 for 16 hours to separate the epidermis and the dermis, and the moisturizing effect of both epidermis and dermis was confirmed.

3. Experimental Results

As shown in Fig. 3, the moisture content (mean SEM: measured by skin) of UV-group, UV + group, BRJ1, BRJ2, BRJ3 and BRJ4 of the composition of the present invention immediately before UV irradiation As a result of measuring the moisture content of the skin after the 6 weeks of feeding of the capacitance in au, the water retention amount of the skin, which was fed with the composition of the present invention, was significantly higher than that of the UV + And the effect of moisturizing enhancement was high.

<Experimental Example 4> The effect of the composition for inhibiting skin aging of the present invention on the serum IgE level

1. Experimental animals and conditions

The same procedure as in Experimental Example 1 was carried out.

2. Experimental method

In the same manner as in Experimental Example 2, the following method was further used to measure serum IgE.

That is, as a method of measuring serum IgE, blood was collected from the orbital artery before sacrifice of an animal, and the total IgE concentration in the serum obtained by centrifuging at 3000xg for 30 minutes at 4 ° C was measured by ELISA.

3. Experimental Results

As shown in FIG. 4, IgE levels (ng / ml) measured in sera of BRJ1, BRJ2, BRJ3 and BRJ4 as the compositions of the present invention were 10.7 ± 5.42, 115.7 The IgE levels of the BRJ1, BRJ2, BRJ3 and BRJ4 groups of the compositions of the present invention were found to be increased by irradiation with ultraviolet light, Was significantly lower than that of UV + group.

<Experimental Example 5> Confirmation of antioxidative effect of the composition for inhibiting skin aging of the present invention

1. Experimental animals and conditions

The same procedure as in Experimental Example 1 was carried out.

2. Experimental method

In order to confirm the antioxidative effect in addition to the above Experimental Example 2, the composition of the present invention was supplementally administered to rats exposed to ultraviolet rays for 6 weeks, and the degree of DNA damage of leukocytes was analyzed by the following method.

That is, in order to confirm the antioxidant effect, degree of damage of erythrocytes induced by photoaging and feeding of the composition of the present invention was measured using the Comet assay.

Whole blood of the animal was collected and mixed with 75 μl of 0.7% LMA (low melting agarose gel), and then a fully frosted slide precoated with 0.5% normal melting agarose (NMA) The suspension of LMA was dispersed evenly, then covered with cover glass and stored in a refrigerator at 4 ° C.

When the gel was hardened, cover glass was peeled off and another layer of 75 μl of 0.7% LMA solution was further coated on the cover glass.

Add 1% Triton X-100 immediately prior to use in a cold alkaline lysis buffer (2.5 M NaCl, 100 mM EDTA, 10 mM tris) and place the slides in a dark room And immersed for 1 hour to loosen the double strand of DNA.

The lysed slide was placed in an electrophoresis tank and unwound by filling in a cold buffer (buffer / 300 mM NaOH, 1 mM Na2EDTA) at 4 캜. After 40 minutes, 25 V / 3003 mA of A voltage was applied and electrophoresis was performed for 20 minutes.

The cells were washed thoroughly with 0.4 M Tris buffer (pH 7.4), stained with ethidium bromide at a concentration of 20 μg / ml, observed under a fluorescence microscope, and each nucleus image sent through a CCD camera And analyzed on a computer equipped with a comet image analyzing system.

DNA damage by H ₂ O ₂ in lymphocytes and inhibition of damage by various extracts were measured by the difference in the tail length of the DNA fragments migrated from the nucleus or in the tail length of the DNA fragment The percent of DNA damage in each of the 100 cells was measured, and each treatment was repeated at least 3 times.

3. Experimental Results

As shown in FIG. 5, in the case of Tail DNA, the degree of damage was increased in UV + compared to the control (UV-), and in the group treated with BRJ1, BRJ2, BRJ3 and BRJ4, And the antioxidant effect was shown.

Based on the above results, the present invention provides a cosmetic composition for inhibiting skin aging, which has superior skin moisturizing ability and wrinkle formation inhibitory ability by containing bee venom and royal jelly which are safe for human body and have an antioxidative effect, And also can be used by food use, and it is possible to obtain a cosmetic composition for skin aging inhibition which is more convenient for consumers to use, and also it is possible to obtain the effect of increasing the income of farm household through development of various products of bee venom and royal jelly .

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments. You can implement the examples. The scope of the present invention is defined by the appended claims, and all differences within the scope of the claims are to be construed as being included in the present invention.

Claims (6)

It contains bee venom that has anti-wrinkle activity and royal jelly having skin moisturizing effect as an active ingredient,
A cosmetic composition for inhibiting skin aging.
The method of claim 1,
The royal jelly is characterized in that it is contained in 10 ^-3 ~ 10 ^-1 wt% based on the weight of the composition,
A cosmetic composition for inhibiting skin aging.
The method of claim 1,
The bee venom is characterized in that it contains 10 ^-10 to 10 ^-8 wt% based on the weight of the composition,
A cosmetic composition for inhibiting skin aging.
4. The method according to any one of claims 1 to 3,
The composition is made of any one selected from powder, granules, capsules or liquids, characterized in that for use in the diet,
A cosmetic composition for inhibiting skin aging.
Dissolving bee venom separated from the bee with water and centrifuging the lyophilized supernatant to prepare a bee venom dried product;
Lyophilizing the raw royal jelly to prepare a royal jelly dried product; And
Comprising; mixing the bee venom dried and the royal jelly dried;
A method for producing a cosmetic composition for inhibiting skin aging.
A method for inhibiting skin aging, characterized by obtaining a skin moisturizing effect and a wrinkle production inhibiting effect by ingesting the cosmetic composition according to any one of claims 1 to 4.

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