WO2016163427A1 - Agent d'amélioration de la croissance de végétaux apte à réduire l'inhibition de croissance provoquée par une humidité excessive, et procédé permettant de réduire l'inhibition de croissance provoquée par une humidité excessive - Google Patents

Agent d'amélioration de la croissance de végétaux apte à réduire l'inhibition de croissance provoquée par une humidité excessive, et procédé permettant de réduire l'inhibition de croissance provoquée par une humidité excessive Download PDF

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WO2016163427A1
WO2016163427A1 PCT/JP2016/061321 JP2016061321W WO2016163427A1 WO 2016163427 A1 WO2016163427 A1 WO 2016163427A1 JP 2016061321 W JP2016061321 W JP 2016061321W WO 2016163427 A1 WO2016163427 A1 WO 2016163427A1
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yeast
soil
growth
improving agent
plant growth
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PCT/JP2016/061321
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English (en)
Japanese (ja)
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正人 船津
建史 白井
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アサヒグループホールディングス株式会社
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Priority to JP2017511037A priority Critical patent/JPWO2016163427A1/ja
Publication of WO2016163427A1 publication Critical patent/WO2016163427A1/fr

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32—Yeast
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00—Botany in general
    • A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants

Definitions

  • the present invention relates to a plant growth improving agent, and more particularly to a growth improving agent that can improve the suppression of plant growth caused by excessive moisture (pF value of 1.5 or less).
  • moisture damage causes crop failure such as a lack of oxygen in the soil due to excess water in the soil in farm fields, etc., resulting in inhibition of rooting.
  • the moisture damage is one of the obstacles in crop cultivation.
  • paddy fields that are no longer used due to rice production adjustments due to the policy of reduction are about 1 million hectares (2003), and it has been tried to cultivate wheat etc. as paddy field rotation fields.
  • the water permeability to the deep part is impaired, and as a result, the surface tends to be stagnated by rain.
  • a disinfectant such as Cruiser (registered trademark) MAXX
  • a disinfectant such as Cruiser (registered trademark) MAXX
  • measures such as deep plowing, high hail cultivation, and use of moisture-resistant varieties are also taken.
  • Etherphon which is marketed as an agricultural chemical and growth regulator.
  • Patent Documents 1 and 2 reducing fertilizers and microorganism-derived reducing mixtures produced using brewer's yeast or the like are known (for example, Patent Documents 1 and 2).
  • the reducing fertilizer of patent document 1 is comprised in the aspect containing microbial material superheated steam treatment material and diatomaceous earth, for example, microbial material superheated steam treatment material is yeast, the extract of yeast, or yeast, for example. It can be obtained by subjecting a mixture containing cell walls, phosphoric acid and potassium to superheated steam treatment (hydrothermal reaction treatment).
  • Patent Document 1 describes that application of the reducing fertilizer can promote the growth of roots of fruit trees and the enlargement of fruits.
  • the microorganism-derived reducible mixture of patent document 2 is comprised including the microorganism material superheated steam treatment thing, the microorganisms which are not performing the superheated steam treatment, or the component of microorganisms.
  • the microbial material superheated steam-treated product according to Patent Document 2 can also be obtained by subjecting yeast or the like to superheated steam treatment.
  • Patent Document 2 describes that the oxidation-reduction potential of soil can be adjusted by applying a microorganism-derived reducing mixture, and as a result, it can contribute to the promotion of absorption of yeast-derived components and soil improvement.
  • This invention is made
  • the present inventor has conducted intensive research on a technique that can reduce moisture damage caused by excessive moisture in the soil and improve plant rooting.
  • the rooting improving agent is a microorganism material superheated steam treatment obtained by subjecting a mixture containing yeast, yeast extract or yeast cell wall, and phosphoric acid or a phosphate compound and potassium or potassium compound to superheated steam treatment.
  • a microorganism-derived mixture in which yeast cell wall powder and diatomaceous earth are mixed.
  • the rooting of a plant when the soil is in an excessively humid state can be improved by mixing the rooting improving agent with the soil.
  • the inventor can improve the suppression of plant growth caused by the soil becoming excessively humid by adding the yeast degradation product adsorbed to the porous material to the soil.
  • the present invention was completed.
  • the gist of the present invention is as follows.
  • a plant growth improving agent comprising one or more yeast degradation products selected from the group consisting of yeast extract, yeast cell wall and yeast cell wall degradation product adsorbed on the porous material.
  • the porous material is one or more porous materials selected from the group consisting of diatomaceous earth, pearlite, zeolite, charcoal and activated carbon.
  • a plant growth improving agent containing one or more yeast degradation products selected from the group consisting of cell wall degradation products is mixed with soil
  • a method for improving the suppression of plant growth caused by the soil becoming excessively humid comprising planting and cultivating a plant in soil mixed with the plant growth improving agent.
  • the porous material is one or more porous materials selected from the group consisting of diatomaceous earth, pearlite, zeolite, charcoal and activated carbon.
  • yeast degradation product is a yeast degradation product obtained by subjecting yeast to a degradation treatment by an autolysis method.
  • FIG. It is a graph showing the relationship between the growth improving agent of an Example or the soil admixture of a comparative example, and root dry matter weight concerning the test example 1.
  • FIG. It is a graph which concerns on the test example 2 and represents the relationship between the growth improving agent of an Example, and a root dry matter weight. It is a graph which concerns on the test example 2 and represents the relationship between the growth improving agent of an Example, and a root dry matter weight. It is a graph which concerns on the test example 2 and represents the relationship between the growth improving agent of an Example, and a root dry matter weight. It is a graph which concerns on the test example 3 and represents the relationship between the growth improving agent of an Example, and a root dry matter weight.
  • FIG. It is a graph showing the relationship between the growth improvement agent of an Example, or the soil mixture of a comparative example, and root dry matter weight concerning the test example 4.
  • FIG. It is a graph which concerns on the test example 5, and represents the relationship between the growth improving agent of an Example, or the soil mixture of a comparative example, and root dry matter weight.
  • the present embodiment relates to a plant growth improving agent (hereinafter also simply referred to as a growth improving agent) that can improve the suppression of plant growth caused by excessive soil moisture, and at least a porous material and a yeast degradation product It is configured to include. Moreover, the yeast decomposition product is contained in the aspect adsorbed by the porous material.
  • a plant growth improving agent hereinafter also simply referred to as a growth improving agent
  • a porous material and a yeast degradation product It is configured to include.
  • the yeast decomposition product is contained in the aspect adsorbed by the porous material.
  • the contained yeast degradation product is a degradation product obtained by performing a degradation treatment on yeast, and is specifically selected from the group consisting of yeast extract, yeast cell wall, and yeast cell wall degradation product. It can be 1 type or 2 types or more.
  • the yeast may be cultured to obtain the yeast degradation product according to the present embodiment, but from the viewpoint of waste utilization and waste disposal cost reduction, beer, whiskey, shochu, sake, miso, soy sauce It is preferable to use yeast obtained as extra waste discharged in the brewing industry.
  • the yeast degradation product can be produced using one or more of mud beer yeast, pressed beer yeast, dry beer yeast, beer yeast suspension, and the like.
  • a self-digestion method that solubilizes cells by using a proteolytic enzyme or the like inherent in yeast cells, solubilizing cells by adding microorganism or plant-derived enzyme preparations Enzymatic decomposition method, Hot water extraction method to solubilize cells by soaking in hot water for a certain period of time, Acid / alkaline decomposition method to solubilize cells by adding various acids or alkalis, Freezing / thawing
  • the freeze-thaw method which crushes a microbial cell by performing this once or more
  • the physical crushing method which crushes a microbial cell by physical irritation, etc. are mentioned.
  • Examples of physical stimulation used in the physical crushing method include ultrasonic treatment, homogenization under high pressure, and grinding by mixing with a solid material such as glass beads.
  • a yeast extract is an extract that can be prepared from a yeast culture and can be obtained, for example, by any of the degradation methods described above.
  • the yeast cell wall is not particularly limited in its preparation method (decomposition method).
  • yeast cell walls obtained as a by-product in the yeast extract preparation process can be used for this embodiment.
  • the degradation product of the yeast cell wall can be obtained, for example, by subjecting the yeast cell wall to enzymatic degradation or hydrothermal reaction according to a known method.
  • Enzymes that can be used for the preparation of yeast cell wall degradation products include glucanase, ⁇ -amylase, ⁇ -amylase, glucoamylase, pullulanase, transglucosidase, dextranase, glucose isomerase, cellulase, naringinase, hesperidinase, xylanase, Hemicellulase, mannanase, pectinase, invertase, lactase, chitinase, lysozyme, inulinase, chitosanase, ⁇ -galactosidase, protease, papain, peptidase, aminopeptidase, lipase, phospholipase, phytase, acid phosphatase, phosphodiesterase, catalase, glucose oxidase, peroxidase, Tannase
  • the yeast degradation product which concerns on this embodiment is the aspect of a mixture
  • the said mixture may be the thing which has not been isolate
  • a material that has not been subjected to separation treatment for example, after performing a decomposition treatment on yeast, the yeast extract and the yeast cell wall are adsorbed to a porous material as they are without being separated into this embodiment. The case where such a growth improving agent is obtained is mentioned.
  • the growth improving agent of this embodiment by containing 2 or more types among yeast extract, a yeast cell wall, and the degradation product of a yeast cell wall, compared with the case where these are contained independently, the growth improvement effect is further improved. Can be increased, which is preferable.
  • the method for decomposing yeast is not particularly limited, but on the other hand, since the growth of the plant can be promoted with a smaller addition ratio to the soil, the yeast degradation product according to this embodiment is prepared by an autolysis method. It is preferable.
  • yeast cell walls or a degradation product thereof and a mixture thereof as yeast degradation products from the viewpoint of treatment of surplus waste discharged in the industry.
  • porous material As a porous material, it can be set as 1 type, or 2 or more types selected from the group which consists of diatomaceous earth, pearlite, a zeolite, charcoal, and activated carbon, for example.
  • Examples of charcoal include charcoal, bamboo charcoal, and rice husk charcoal.
  • diatomaceous earth it is preferable to contain diatomaceous earth as a porous material from the viewpoint of having a track record as an agricultural material.
  • the growth improving agent of the present embodiment can be prepared by mixing the aforementioned yeast degradation product and porous material.
  • the mixing method is not particularly limited, and can be appropriately set by those skilled in the art.
  • the yeast degradation product is adsorbed in a porous manner.
  • the mixing ratio of the yeast degradation product and the porous material is not particularly limited, but from the viewpoint of further promoting the growth of the plant, for example, the ratio (adsorption rate) of the yeast degradation product to the whole growth improving agent of this embodiment is 0. It is preferably 5% (W / W) or more.
  • the adsorption rate is more preferably 1% (W / W) or more, still more preferably 1 to 30% (W / W), still more preferably 10 to 20% (W / W), and even more preferably. 10 to 15% (W / W).
  • the upper limit of the adsorption rate is not particularly limited, but is preferably 40% (W / W) or less from the viewpoint of manufacturing cost and labor.
  • the growth improving agent of the present embodiment is mixed with soil such as a farm field, and can improve the growth inhibition of plants called moisture damage caused by the soil becoming overhumid. Specifically, the growth improving agent of the present embodiment suppresses the influence caused by the soil becoming excessively humid, and improves plant growth.
  • the overhumid soil refers to a state in which the moisture contained in the soil is excessive, for example, the pF value representing the degree of strength that the water in the soil is attracted by the capillary force of the soil. State that is 1.5 or less.
  • the state in which the soil is excessively humid may be temporary, and varies depending on the type of plant, the type of soil, the temperature, the degree of excessive humidity, the growth stage at the time of encountering excessive humidity in the plant, etc. It is possible to sufficiently improve the moisture damage (growth suppression) of plants due to temporary overhumidity by adding the growth improver to the soil.
  • Specific examples of moisture damage due to temporary overhumidity include pumpkin, cucumber, tomato, biman, onion, cabbage, Chinese cabbage, burdock (6-7 leaves), Japanese radish, green beans, etc. It is known that wet damage will occur in celery, spinach, leek, burdock (2-3 leaves), carrots, sweet potatoes, etc.
  • the pF value can be measured based on the tensiometer method.
  • the tensiometer method is described in, for example, “Soil Environment Analysis Method”, supervised by the Japan Soil Fertilizer Society, edited by the Soil Environment Analysis Method Editorial Committee, published by Hirotomo, 1997, first edition, pages 59-62. Yes.
  • the overhumid soil which concerns on this embodiment is the state (pF value 1.5 or less) in which the water
  • the soil to which the growth improving agent of this embodiment is applied is not particularly limited and can be appropriately set by those skilled in the art. Although those skilled in the art can naturally understand, in this specification, soil refers to a component that is derived from rocks and plants and can be used to maintain vegetation. It is also a concept that includes materials used for cultivation such as soil collected from peanuts, peat moss, coco pate, vermiculite, humus, compost, and charcoal.
  • the growth improving agent of the present embodiment is a soil having a pF value of 1.5 or less (over-humid soil, and also includes a case where it temporarily falls within the range as described above) and can grow a plant. Improve plant growth.
  • a preferable aspect in which the growth improving agent of the present embodiment is mixed is a case where the pF value is greater than 0 and 1.5 or less, and a more preferable aspect is a case where the pF value is greater than 0 and 0.5 or less.
  • the method of using the growth improving agent of the present embodiment is not particularly limited, and can be appropriately set by those skilled in the art.
  • the application time is not limited, and the growth improving agent of the present embodiment can be used in the soil before planting (seeding seeds or planting seedlings).
  • the pF value may not be 1.5 or less when the growth improving agent of the present embodiment is mixed with soil.
  • the amount and ratio mixed with the overhumid soil can be appropriately set and are not particularly limited.
  • mixing of soil (cultured soil) and the growth improving agent of the present embodiment is possible. It is preferable to mix in the range of 0.02 to 1.0% (W / V). Further, it is more preferable to mix the growth improving agent of this embodiment in a range of 0.03 to 0.6% (W / V), and it is preferable to mix in a range of 0.03 to 0.3% (W / V). Even more preferred.
  • By mixing in the range of 0.02 to 1.0% (W / V) plant growth can be further promoted.
  • the soil volume per 10a is assumed to be 100t with a soil layer of 10cm and a temporary specific gravity of 1, and mixing at 0.02 to 1.0% (W / W), similar to the above range, It is preferable for the same reason.
  • the yeast degradation product is 0.5 to 30% (W / W) (more preferably 10 to 20% (W / W), more preferably still, per growth improving agent. 10 to 15% (W / W)) of the growth improving agent of this embodiment, the mixing ratio of the soil and the growth improving agent of this embodiment is 0.02 to 1.0% (W / V) ) (0.02-1.0% (W / W) for fields) (more preferably 0.03-0.6% (W / V), even more preferably 0.03-0.3% ( W / V)).
  • the amount of cultivation in a field or the like to which the growth improving agent of this embodiment is applied is not particularly limited.
  • spinach 25,000 to 200,000 shares / 10a (preferably 50,000 to 150,000 shares / 10a)
  • sweet potatoes 1,000 to 10,000 shares / 10a (preferably 2000 to 5000 shares / 10a)
  • wheat In the case of 5,000 to 500,000 strains / 10a (preferably 80,000 to 400,000 strains / 10a)
  • green soybeans 2000 to 30,000 strains / 10a (preferably 5000 to 15,000 strains / 10a)
  • rice seedlings In the case of: 50 g to 300 g / one seedling box (preferably 130 g to 180 g / one seedling box).
  • the growth suppression (moisture damage) of the plant resulting from the soil becoming an overhumid state is improved by mixing the said growth improving agent with soil. Can do. Therefore, it can be expected that plants can be grown healthy while improving growth inhibition (wet damage) in diversion of paddy fields that are left uncultivated or in fields where hard disks are formed.
  • a plant cultivated in a field or the like to which the growth improving agent of the present embodiment is applied is not particularly limited, and can be appropriately set by those skilled in the art. Specific examples include monocotyledonous plants such as wheat, leeks and onions, and dicotyledonous plants such as sweet potato and spinach, which have been tried in the paddy field cropping field.
  • leek, onion, sweet potato, and spinach are known to be vulnerable to moisture damage compared to other cereals and vegetables, but by mixing the growth improving agent of this embodiment with soil, The growth of these crops in can be improved. Furthermore, by adding the growth improving agent of this embodiment to the soil, green soybeans (soybeans) that are known to be less susceptible to moisture damage than other cereals and vegetables, as well as sweet potatoes, and Asia including Japan. It is also possible to improve the growth of rice, which is a representative crop in the field (especially rice in the seedling stage before planting).
  • Test Example 1 The test varieties were spinach 'night-extraction parc' and tested in a greenhouse owned by Asahi Group Holdings in Moriya, Ibaraki. Sowing was performed by filling a 72-hole cell tray with the seedling culture medium of Takii Seedling Co., Ltd. The seedlings were raised on a seedling terrace (closed-type seedling production system) at 25 ° C for 12 hours, and irrigation was conducted once a day. The pots were raised in a No. 5 polypot and grown in a plastic house for 20 days.
  • test plots were: A) Control plot (no soil mixing), B) Diatomaceous earth (powder) only, C) Yeast (powder), D) Yeast adsorbed on diatomaceous earth, E) Yeast extract (powder), F) Diatomaceous earth Adsorbed yeast extract (Example 1), G) Yeast cell wall (powder), H) Yeast cell wall adsorbed on diatomaceous earth (Example 2), I) Enzymatic degradation product (powder) of yeast cell wall, J) Diatomaceous earth An enzyme degradation product (Example 3) of the adsorbed yeast cell wall was set.
  • yeast extract obtained by yeast self-digestion method as yeast extract ("Meast” (Asahi Food and Healthcare Co., Ltd.)
  • yeast self-digestion method and enzyme treatment as yeast cell wall
  • yeast cell wall (“YPR” (Asahi Food and Healthcare Co., Ltd.)
  • YPR Asahi Food and Healthcare Co., Ltd.
  • an enzymatic degradation product of the yeast cell wall the yeast cell wall obtained by the yeast self-digestion method was subjected to the enzymatic degradation method.
  • the obtained degradation product (“Toyo crop word ⁇ SP powder>” (Asahi Food and Healthcare Co., Ltd.) was used.
  • yeast extract, yeast cell wall, or enzyme degradation product of yeast cell wall were It was made to adsorb
  • the soil pF value is adjusted according to the irrigation method, and two types of soil moisture conditions, pF1.5 to 2.7 (water irrigation / suitable humidity, the same shall apply hereinafter) and pF0 to 0.3 (bottom flooding / superhumidity, the same shall apply hereinafter) Cultivated in
  • Each material containing the growth improving agent of the examples according to the above B) to J) is 0.06% in the mixing ratio of the material to the soil (hereinafter also referred to as soil miscibility. The same applies to other test examples). (W / V).
  • the result of measuring the dry weight of the root after the test is shown in FIG.
  • the root mass was significantly decreased in the over-humidity / immiscible group compared with the appropriate-humidity / immiscible group.
  • each material growth improving agent of Examples 1 to 3 in which yeast extract, yeast cell wall and enzyme degradation product of yeast cell wall were adsorbed on diatomaceous earth in an overhumid state was mixed with soil, it was compared with the overhumidity / non-mixed group. It was found that the root mass increased significantly.
  • Test Example 2 was conducted in order to confirm appropriate amounts such as the type of yeast degradation product, the ratio of yeast degradation product, and the soil admixture of the growth improver.
  • test varieties were spinach 'night-extraction parc' and tested in a greenhouse owned by Asahi Group Holdings in Moriya, Ibaraki. Sowing was performed by filling a 72-hole cell tray with the seedling culture medium of Takii Seedling Co., Ltd. The seedlings were raised on a seedling terrace (closed-type seedling production system) at 25 ° C for 12 hours, and irrigation was conducted once a day. The pots were raised in a No. 5 polypot and grown in a plastic house for 30 days.
  • yeast degradation products yeast cell walls derived from brewer's yeast (“YPR”, Asahi Food and Healthcare Co., Ltd.), yeast cell wall degradation products obtained by allowing enzymes to act on yeast cell walls obtained by the self-digestion method (good harvest) Story ⁇ >, Asahi Food and Healthcare Co., Ltd., hereinafter also referred to as cell wall degradation product (self-digestion method), yeast cell wall degradation product obtained by allowing enzymes to act on yeast cell wall obtained by enzymatic degradation method (Abundant crop word ⁇ green>, Asahi Food and Healthcare Co., Ltd., hereinafter also referred to as cell wall degradation product (enzymatic degradation method)).
  • the growth improving agents of the examples were prepared by adsorbing these yeast cell walls or cell wall degradation products to diatomaceous earth at a ratio of 10% (W / W) or 20% (W / W) as a whole of the growth improving agent (adsorption). Rate: 10% (W / W) or 20% (W / W)).
  • the growth improving agent obtained using the yeast cell wall is collectively referred to as the growth improving agent of Example 4.
  • the growth improving agent obtained by using the cell wall degradation product self-digestion method
  • the growth improving agent obtained by using the cell wall degradation product (enzymatic degradation method).
  • the growth improving agent of Example 6 are collectively referred to as the growth improving agent of Example 6.
  • the soil admixture of the growth-improving agent is 0.03% (W / V), 0.06% (W / V), 0.3% (W / V), or 0.6% (W / V).
  • the soil pF value was adjusted by the irrigation method, and cultivation was carried out under the two soil moisture conditions of each test section, pF1.5 to 2.7 (jouro irrigation / suitable humidity) and pF0 to 0.3 (bottom flooding / overhumidity).
  • FIGS. The results of measurement of root dry matter weight after completion of the test are shown in FIGS. Regarding the growth improving agents of Examples 4 and 5, especially when the mixture was mixed with soil at a soil degradation rate of 0.03 to 0.3% (W / V) with a yeast degradation product ratio of 10% (W / W), The root growth improvement effect was confirmed. Moreover, about the growth improving agent of Example 6, when the ratio of a yeast degradation product is 10% (W / W) and soil mixing rate is 0.6% (W / V), the ratio of a yeast degradation product is 20% (W / W). In the case of W) and the soil admixture ratio of 0.03 to 0.3% (W / V), the root growth improving effect was confirmed particularly in overhumid soil.
  • the yeast cell wall and the enzymatic degradation product of the yeast cell wall differ in the ratio of the appropriate yeast degradation product and the soil mixing ratio depending on the degradation method for yeast when obtaining the yeast cell wall.
  • yeast cell walls and cell wall degradation products autolysis method
  • plants can be used even if the proportion of yeast degradation products added to the soil is smaller than when cell wall degradation products (enzymatic degradation method) are used. It can be understood that the growth of can be promoted.
  • the ratio of yeast degradation products has an optimum value in the range of 10 to 20% (W / W) /.
  • Test Example 3 The test varieties were spinach 'night-extraction parc' and tested in a greenhouse owned by Asahi Group Holdings in Moriya, Ibaraki. Sowing was carried out by filling a 72-hole cell tray with the seedling culture soil of Takii Seedling Co., Ltd. The seedlings were raised on a seedling terrace (closed-type seedling production system) at 25 ° C for 12 hours, and irrigation was carried out once a day so that the irrigation was in an overhumid state of pF0 to 0.3. The pots were raised in a No. 5 polypot and grown in a plastic house for 20 days.
  • Example 7 Hokkaido diatomaceous earth (“nano-K” (Cross Farm Co., Ltd.)) and yeast cell wall (“YPR” (Asahi Food and Healthcare Co., Ltd.)) in a total ratio of 10% (W / W) Adsorption was carried out to obtain a growth improving agent of Example 7 (adsorption rate: 10% (W / W)).
  • diatomaceous earth Standard Supercell” (Tokyo Kogyo Trading Co., Ltd.)
  • the growth improver of Example 8 was obtained, and two test plots were set, and the growth improver of Examples 7 and 8 was added to each test plot as 0.06% (W / V).
  • Test Example 4 The test varieties were spinach 'night-drawn bulk' and tested in a greenhouse owned by Asahi Group Holdings in Moriya, Ibaraki Prefecture. Sowing was carried out by filling a 72-hole cell tray with the seedling culture soil of Takii Seedling Co., Ltd. The seedlings were raised on a seedling terrace (closed-type seedling production system) at 25 ° C for 12 hours, and irrigation was carried out once a day so that the irrigation was in an overhumid state of pF0 to 0.3. The pots were raised in a No. 5 polypot and grown in a plastic house for 20 days.
  • Yeast cell wall degradation product (“YPR”, Asahi Food and Healthcare Co., Ltd.) is adsorbed to pearlite, charcoal (rice husk charcoal), activated carbon, or zeolite so that the total amount is 10% (W / W).
  • the growth improving agents of Examples 9 to 12 were obtained (adsorption rate: 10% (W / W)).
  • Each test group was set for the growth improving agents of Examples 9 to 12, and each test group was mixed at a soil mixing ratio of 0.06% (W / V).
  • a section in which 0.06% (W / V) was mixed with soil without adsorbing yeast cell wall degradation products was set for each material.
  • a test group that was grown with no material admixture, appropriate humidity and excessive humidity was set as a control group.
  • the result of measuring the dry weight of the root after the test is shown in FIG. It was confirmed that all of the growth improving agents of Examples 9 to 12 obtained by adsorbing the yeast cell wall (YPR) to pearlite, charcoal, activated carbon, or zeolite had a growth improving effect under excessively humid conditions. On the other hand, the growth improvement effect was not seen by all each material which is a porous material alone. From the results, it can be understood that the porous material according to the growth improving agent of the present invention is not limited to diatomaceous earth, and the same effect can be obtained when other porous materials are used.
  • Yeast cell walls (“YPR” (Asahi Food and Healthcare Co., Ltd.)) were adsorbed to diatomaceous earth or zeolite so as to have a ratio of 10% (W / W) as a whole, and the growth improving agents of Examples 13 and 14 were obtained. (Adsorption rate: 10% (W / W)).
  • a test plot was set and mixed at a rate of 0.06% (W / V) per mixture of soil and growth improver.
  • the test plot which set the mixing ratio of soil and the growth improving agent to 0.12% (W / V) was also set.
  • test plot was set in which diatomaceous earth or zeolite and yeast cell wall were mixed with soil without adsorbing the yeast cell wall to diatomaceous earth or zeolite, respectively. Further, as a control group, a test group that was grown without mixing materials under superhumid conditions was set.
  • the result of measuring the dry weight of the root after the test is shown in FIG.
  • the root mass was significantly increased in any of the test plots mixed with the growth improving agent of Example 13 or 14 prepared by adsorbing yeast cell walls to diatomaceous earth or zeolite.
  • the values of the dry weight of the control group and the root dry matter were almost the same. From the above results, it was suggested that the yeast extract and its degradation products would not show growth improvement effect even if mixed with soil unless adsorbed on porous materials.
  • the test varieties were edamame: 'Kitanosato', rice: 'Nihonbare', onion: 'Quanzhou Chuo', and green onion: 'Ishikura Ippon' in a greenhouse owned by Asahi Group Holdings in Moriya, Ibaraki. I did it.
  • the seedlings were raised on a seedling terrace (closed-type seedling production system) at 25 ° C for 12 hours, irrigation once a day by bottom irrigation. Sowing is done in a 72-hole cell tray. A nursery soil was used. The cultivation period was 21 days for green soybeans, 17 days for rice, 30 days for onions, and 45 days for onions.
  • the soil pF value was around 0.5 for the seed sowing medium of the green soybean Takii Seedling Co., Ltd.
  • the growth-improving agent of the example was prepared in the same manner as in Example 2 or 3 using the yeast cell wall or its enzymatic degradation product.
  • the test plots for green soybean (soybean), rice, onion, and green onion were controls (soil admixture of material 0% (W / V)), soil admixture of the growth improver (yeast cell wall) of Example 15 0.06% ( Two test zones (W / V) were set.
  • test groups were established: the two test groups and a test group having a soil admixture of 0.06% (W / V) of the growth improving agent (cell wall degradation product) of Example 16.
  • the survey was conducted in 12 replicate strains, and repeated 3 times in 1 test area.
  • FIG. 10 An example of green soybeans after the test is shown in FIG.
  • the above-mentioned dry weight was significantly increased in both the growth improving agent 0.06% group of Example 15 and the growth improving agent 0.06% group of Example 16 as compared with the control group (FIG. 9).
  • the underground dry matter weight in the growth improving agent 0.06% group of Example 15 was particularly increased compared to the control group (FIG. 10).
  • the germination rate was 0.06% for the growth improving agent of Example 15 and 0.06% for the growth improving agent of Example 16, both higher than the control, and significantly higher at the 5% level for the 0.06% growth improving agent of Example 15. .
  • Example 15 In rice, the above-mentioned dry weight and underground dry weight increased significantly in both the 0.06% growth improvement agent of Example 15 and the 0.06% growth improvement agent of Example 16 as compared to the control group (FIG. 11 to FIG. 11). 13).
  • the dry matter weight in the underground portion was significantly increased in the growth improving agent 0.06% section of Example 9 (FIGS. 14 to 15).
  • the dry weight of the above-ground part and the dry part of the underground part significantly increased in the 0.06% growth improving agent of Example 9 (FIGS. 16 to 18).
  • the test area is a material in which the yeast cell wall and yeast extract are mixed at 4: 6 and adsorbed on diatomaceous earth (hereinafter referred to as material (1)) and a material in which the yeast cell wall is adsorbed on diatomaceous earth (hereinafter referred to as material (2)).
  • material (1) a material in which the yeast cell wall and yeast extract are mixed at 4: 6 and adsorbed on diatomaceous earth
  • material (2) a material in which the yeast cell wall is adsorbed on diatomaceous earth
  • 2 materials were prepared and used as growth improving agents in the examples.
  • the growth improving agents of these examples were prepared by adsorbing the yeast degradation product on diatomaceous earth so that the ratio (adsorption rate) of the yeast degradation product to the whole mixture was the value shown in Table 1.
  • the test group which mixed by the mixing rate shown in Table 2 or Table 3, respectively was set.
  • the control group was a material-unmixed group. Since the material (1) and the material (2) were tested on different seedling terraces, the control zone was also set for each material (1) and (2).
  • the test was conducted with 5 strains ⁇ 3 replicates in 1 test section.
  • yeast is decomposed by various methods to extract a yeast extract, and the extraction rate varies depending on the method, and is about 30 to 70%. In general, considering the economy and quality, the extraction rate is about 60%.
  • the material (1) in which the yeast cell wall and the yeast extract are blended at 4: 6 is equivalent to a material obtained by decomposing yeast and adsorbing it as it is on diatomaceous earth.
  • Tables 2 and 3 The results are shown in Tables 2 and 3.
  • an example of the photograph of the rice cultivated in the test area which mixed the material (1) of adsorption rate 1% (W / W) is shown in FIG.

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Abstract

La présente invention vise à fournir une nouvelle technique grâce à laquelle l'inhibition de la croissance de végétaux provoquée par une humidité excessive du sol (dégâts d'humidité) peut être réduite. L'invention décrit un agent d'amélioration de la croissance de végétaux apte à réduire l'inhibition de la croissance de végétaux provoquée par une humidité excessive du sol, ledit agent d'amélioration de la croissance de végétaux comprenant un matériau poreux et un ou plusieurs types de produits de décomposition de levure qui sont choisis dans le groupe constitué par un extrait de levure, une paroi cellulaire de levure et un produit de décomposition de paroi cellulaire de levure et adsorbés par le matériau poreux.
PCT/JP2016/061321 2015-04-07 2016-04-06 Agent d'amélioration de la croissance de végétaux apte à réduire l'inhibition de croissance provoquée par une humidité excessive, et procédé permettant de réduire l'inhibition de croissance provoquée par une humidité excessive WO2016163427A1 (fr)

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