WO2016149883A1 - Uses of multipolar microkinetic drinking water in preparing drink, healthcare or medicament used for reducing blood pressure - Google Patents

Uses of multipolar microkinetic drinking water in preparing drink, healthcare or medicament used for reducing blood pressure Download PDF

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WO2016149883A1
WO2016149883A1 PCT/CN2015/074761 CN2015074761W WO2016149883A1 WO 2016149883 A1 WO2016149883 A1 WO 2016149883A1 CN 2015074761 W CN2015074761 W CN 2015074761W WO 2016149883 A1 WO2016149883 A1 WO 2016149883A1
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frequency electromagnetic
electromagnetic wave
water
drinking water
kinetic energy
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PCT/CN2015/074761
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French (fr)
Chinese (zh)
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王卫星
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欢乐海（北京）水业科技有限公司
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Priority to AU2015387923A priority Critical patent/AU2015387923A1/en
Priority to PCT/CN2015/074761 priority patent/WO2016149883A1/en
Publication of WO2016149883A1 publication Critical patent/WO2016149883A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/30—Treatment of water, waste water, or sewage by irradiation
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F1/00—Treatment of water, waste water, or sewage
    • C02F1/48—Treatment of water, waste water, or sewage with magnetic or electric fields

Definitions

  • the invention belongs to the technical field of water treatment, and particularly relates to the use of multi-pole micro-kinetic energy drinking water in preparing beverages, health care products or medicines for lowering blood pressure.
  • Water is an inorganic substance composed of two elements of hydrogen and oxygen, and is a colorless and odorless transparent liquid under normal temperature and normal pressure. Water is one of the most common substances, an important resource for all life, including human beings, and the most important component of living things. Water plays an important role in the evolution of life. Humans have long begun to understand water, and water is regarded as a basic component in the ancient and simple material view of the East and West.
  • the water molecule itself has its special structural shape, it is composed of two hydrogen and one oxygen ion combined at an angle of 104.5. It is an ionization system with an electronic "magnetic polarity". Water molecules exhibit solid crystals below zero and below zero, which we call ice. However, water is not completely liquid at a temperature of from zero to 60 ° C, but exists in the form of a liquid crystal. However, if the temperature is higher than 60 ° C, the liquid crystal molecules will be destroyed.
  • the form of the diluted solute molecules can be contained in its liquid crystals, which is a generally understood dissolution process.
  • the new discovery is that when a solute molecule is separated from a water molecule by some means (eg, through multiple dilutions and oscillations), the water molecule can still maintain a "shape" memory of the solute molecule. When the body cells are exposed to such water molecules, the "shape" memory of the solute molecule is treated as a true solute molecule, which is the cornerstone of homeopathic therapy with more than 200 years of history.
  • Water is composed of two elements of hydrogen and oxygen, but does not exist in nature as a single water molecule. Instead, a group of water molecules (H 2 O) n is present in nature (ie, the above liquid state). Crystallization), the group of water molecules may be chain, ring, agglomerate or grape-like, with at least five water molecules, and more than a dozen, tens to hundreds.
  • Electromagnetic waves have been used to treat water molecules to increase the energy level of water molecules or to disinfect or prevent and remove dirt.
  • these treatment methods do not control the effect of the treatment well, and the effect of the treated water is mostly limited to drinking, and there is no other additional advantageous effect.
  • the present invention provides a multi-pole micro-kinetic energy drinking water obtained by electromagnetic wave non-contact treatment, a preparation method thereof and use thereof.
  • the present invention provides a multi-pole micro-kinetic energy drinking water prepared by non-contact treatment of drinking water raw water by electromagnetic waves, and the ultraviolet absorption peak is short-waved compared with the raw water of drinking water.
  • the direction shift is 25 nm to 40 nm, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the multi-pole micro-kinetic energy drinking water of the present invention is best consumed or used within 72 hours after preparation.
  • the present invention provides a method for preparing the above-mentioned multi-pole micro-kinetic energy drinking water, which comprises non-contact treatment of drinking water raw water by using electromagnetic waves of low, medium and high frequencies, wherein the frequency of low-frequency electromagnetic waves
  • the range is 30-100 kHz
  • the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz
  • the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
  • the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Cambodia glacier mineral water, Nongfu Spring, Wahaha, and the like.
  • the low frequency electromagnetic wave has a frequency in the range of 45-75 kHz.
  • the intermediate frequency electromagnetic wave has a frequency range of 600-720 kHz.
  • the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are a sine wave, a square wave, a sharp wave, a sawtooth wave or a trapezoidal wave.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform may be the same or different, preferably the same.
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by the electromagnetic wave non-contact treatment of the drinking water raw water treatment by using three different frequencies of low, medium and high frequencies, and then the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave are simultaneously processed.
  • the time for simultaneous treatment using the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes; the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes. .
  • the prepared ultraviolet light absorption peak of the multi-pole micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably to the short-wave direction by 28 nm to 30 nm, and most preferably to the short-wave direction. 30 nm or 28 nm.
  • the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
  • a method and apparatus for generating electromagnetic waves are conventional techniques in the art.
  • the present invention provides the use of the above-described multi-pole micro-kinetic energy drinking water in the preparation of various mineral water, purified water, beverages, health care products or medicines.
  • the beverage is a functional beverage and can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.
  • the health care product or drug can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid lowering and blood urea lowering.
  • the invention adopts electromagnetic wave non-contact type treatment water, without adding any additives, is simple to manufacture, has high production efficiency and low cost.
  • the multi-pole micro-kinetic energy drinking water of the present invention is more than the drinking water before treatment.
  • the original water produced a significant shift in the ultraviolet absorption peak to the short-wave direction.
  • the spectral intensity of the fluorescent radiation was greatly improved in the 300-400 nm spectrum, and more extranuclear electrons were in the high-energy non-radiative energy level.
  • Animal experiments have proven to have anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea reduction.
  • FIG 1 shows the effect of water in different treatment groups on peroxide-induced DNA damage
  • FIG. 1 shows the effect of differently treated media on cells
  • 3 is an absorption spectrum distribution of the ordinary drinking water raw water and the multi-pole micro kinetic energy of the present invention obtained by using the ordinary drinking water raw water;
  • Figure 6 is a fluorescence steady state transient test result of ordinary drinking water (raw water) and its multi-pole micro-kinetic energy drinking water;
  • Figure 7 is a fluorescence steady state transient test result of Evian natural mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 8 shows the fluorescence steady-state transient test results of Vietnamese glacial mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
  • Figure 9 shows the results of repeatability test of three samples of multi-pole micro-kinetic energy drinking water made from raw water of Georgia glacial mineral water;
  • Figure 10 is a graph showing the effect of multipolar kinetic energy drinking water on blood pressure systolic blood pressure (SBP) in SHR spontaneously hypertensive rats;
  • SBP blood pressure systolic blood pressure
  • Figure 11 is a graph showing the effect of multipolar kinetic energy drinking water on blood pressure diastolic blood pressure (DBP) in SHR spontaneously hypertensive rats;
  • DBP blood pressure diastolic blood pressure
  • Figure 12 is a graph showing the effect of multipolar kinetic energy drinking water on the mean blood pressure (MBP) of SHR spontaneously hypertensive rats;
  • Figure 13 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on heart rate (HR) in SHR spontaneously hypertensive rats;
  • Figure 14 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the body weight of SHR spontaneously hypertensive rats ring;
  • electromagnetic waves of three different frequencies of low, medium and high are applied in a non-contact manner, wherein the frequency of the low frequency electromagnetic wave is 30 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550 kHz, and the frequency range of the high frequency electromagnetic wave is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both sinusoidal waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 100 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 725 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • electromagnetic waves of low, medium and high frequencies are applied non-contactly, wherein the frequency of the low frequency electromagnetic waves is 45 kHz, the frequency range of the intermediate frequency electromagnetic waves is 600 kHz, and the frequency range of the high frequency electromagnetic waves is 300 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 75 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 425 MHz. .
  • the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sharp waves, and the electromagnetic wave propagation directions of the two are the same.
  • the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
  • the multi-pole micro-kinetic energy drinking water prepared in Examples 1-4 was tested according to the Standard Test Method for Drinking Water (GB/T 5750-2006), and the test results were basically the same. The results are shown in Table 1.
  • the indicators of the micro-kinetic energy drinking water of the present invention all meet the requirements of the Sanitary Standard for Drinking Water (GB5749-2006).
  • Test substance Micro-kinetic energy drinking water prepared in Example 3
  • Animals Kunming species of white mice were provided by the Laboratory Animal Resources Research Institute of China Food and Drug Control Research Institute (Qualification No.: SCXK (Beijing) 2009-0017), SPF Grade, body weight 18.0 ⁇ 22.0g, before the test, the animals fasted overnight, free to drink water.
  • Dose grouping The test substance was set at a dose of 20000 mg/kg body weight, and 10 female and male animals. Grouped by sex. The dose was calculated by oral gavage at 0.1 ml/10 g body weight.
  • the oral LD 50 of the test article for both female and male mice is greater than 10000 mg/kg body weight, which is an actual non-toxic grade.
  • Plasmid DNA pET28a plasmid was amplified and extracted in DH5a E. coli (plasmid extraction kit was purchased from Promega);
  • EDTA is provided by Sinopharm Chemical Reagent Co., Ltd.;
  • Electrophoresis instrument Liuyi Instrument Factory
  • Test substance Micro-kinetic energy drinking water prepared in Examples 3 and 4
  • Group I untreated group, adding untreated sterile water to the reaction system;
  • Group II the kinetic energy drinking water prepared in Example 3;
  • Group III The kinetic energy drinking water prepared in Example 4.
  • reaction materials were added in the following order, and the final volume of the reaction system was 10 ⁇ L:
  • reaction was stopped by adding 1 ⁇ L of 5 mM EDTA and 2.2 ⁇ L of loading buffer at 0.5 hour and 1 hour, respectively.
  • the redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment are shown in Figure 1.
  • the experiments were divided into three groups. The grouping method is described in the scheme. The two time points of each group were: 0.5 hours and 1 hour, and the brightness of DNA represented the DNA content.
  • the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no effect of preventing or accelerating DNA damage and cleavage due to water treatment.
  • Vitamin C undergoes redox reaction with divalent copper ions, accompanied by peroxide formation. Peroxide can cause damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band.
  • the results of this experiment showed that the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no industrial effect of preventing or accelerating DNA damage and cleavage due to water treatment. It is indicated that the multipolar micro kinetic energy of the present invention has no effect on peroxide-induced DNA damage.
  • FAM-aptamer purchased from Shanghai Shenggong Bioengineering Co., Ltd.;
  • the cell culture medium adherently cultured in a 96-well plate was changed to medium A and medium B containing FAM-C6-8 aptamer, cultured at 37 ° C for 30 min, washed once with PBS, and observed under a fluorescence microscope.
  • Fig. 2 There is no difference in cell morphology between the treated group and the untreated group.
  • the cells are white-fluorescent, the cell membrane is intact, and no obvious FAM-labeled C6-8aptamer green fluorescence is observed in the cells.
  • the FAM-labeled C6-8 aptamer can enter the cell and bind to the protein inside the cell, and emit green fluorescence after excitation at 530 wavelength. And the results in the figure see the processing group There is no difference between the cells in the untreated group and the cells, which are autofluorescence, indicating that the cell membrane is intact.
  • the multipolar microkinetic energy technology of the present invention has no effect on cell morphology and cell membrane integrity.
  • Glacier Mineral Water Purchased at Carrefour Supermarket
  • Ultrapure water made by the German Sartorius ultrapure water preparation system
  • Example 3 The ordinary drinking water raw water used in Example 3 and the micro-kinetic energy drinking water prepared in Example 3 were placed in an experimental vessel, and the absorption of the spectral light spectrum of the standard light source of the sample of 200 nm to 800 nm was scanned, and the experimental results were recorded.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in an ultraviolet intensity detecting system, the ultraviolet light source illuminates the water to be tested, and the photodetector Thorlab DET10A/M is read by an oscilloscope MS04104 to read ultraviolet rays that have not passed the water to be tested.
  • the intensity value is read by the value of the ultraviolet intensity of the water to be tested, continuously tested continuously for 72 hours, and the amount of change in ultraviolet intensity during the recording.
  • Example 3 The drinking water made from the Vietnamese glacial mineral water in the same manner as in Example 3 is placed in the ultraviolet intensity detecting system, and the step (2) is repeated to record the amount of change in the ultraviolet intensity during the recording.
  • Multi-pole micro-kinetic energy drinking water made from ordinary water
  • Multi-pole micro-kinetic energy drinking water made from Evian natural mineral water
  • Multi-pole micro-kinetic energy drinking water made from Vietnamese glacial mineral water
  • the absorption spectrum absorption peak of the multi-pole micro-kinetic energy drinking water of the present invention is shifted to a short wave by about 30 nm;
  • Glacier Mineral Water Purchased at Carrefour Supermarket
  • Ultrapure water made by the German Sartorius ultrapure water preparation system
  • Test environment temperature 22 ° C, humidity 37%.
  • Example 3 The micro-kinetic energy prepared in Example 3 is placed in a sample cell of a fiber optic spectrometer, and an excitation pulse is given to the sample by a 266 nm pulsed ultraviolet laser, and the spectral spectrum of the sample is used to measure the emission spectrum of the sample. Fluorescence emission spectrum of the sample.
  • Example 3 The ordinary drinking water raw water used in Example 3 was placed in the fiber optic spectrometer sample cell, and step 2 was repeated to record the experimental data.
  • the fluorescence steady-state transient test results of ordinary drinking water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 6.
  • the fluorescence steady-state transient test results of Evian natural mineral water and its multi-pole micro-kinetic drinking water are shown in Figure 7.
  • the fluorescence steady-state transient test results of Vietnamese glacial mineral water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 8.
  • the glacial mineral water in Moscow was tested for repeatability by taking three samples of multi-polar micro-kinetic energy drinking water made of raw water. The results are shown in Figure 9.
  • the multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;
  • the positive drug amlodipine besylate was produced by Guangdong Pi Di Pharmaceutical Co., Ltd., batch number 20140401.
  • Feeding conditions Experimental animal facilities continue to maintain barrier environmental standards.
  • the control range of the main environmental indicators room temperature 20 ⁇ 26 ° C, the daily temperature difference ⁇ 4 ° C.
  • the relative humidity is 40 to 70%.
  • Minimum air exchange times 15 times / hour, light illumination: dark 12h: 12h.
  • the animals are housed in a polypropylene rat group box.
  • the cage specifications are: 545*395*200mm 3 , 5 per box, and the feeding space meets the minimum space required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Provisions. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.
  • Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.
  • Sample collection The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).
  • Test indicators animal general observation, weight, growth status, blood pressure, heart rate.
  • the daily dose of adult is 10mg
  • the human body weight is calculated according to 60kg, which is 0.167mg/kg, 2mg/kg is about 12 times of human dose, prepared with 0.5% CMC-Na); kinetic energy plus amlodipine besylate group
  • the dose of amlodipine besylate was also 2 mg/kg, which was prepared with kinetic energy.
  • the weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: Kolmogorov-Smirnov method should be used for normal test, Levene median method for homogeneity test of variance, if P>0.05, one-way ANOVA method, if normal and variance The homogeneity test failed (P ⁇ 0.05), then a non-parametric Mann-Whitney test was required.
  • the heart rate of the animal has no effect, and it can be judged that the kinetic energy has the function of assisting blood pressure lowering; if the blood pressure of the amlodipine besylate group is lower than that of the amlodipine besylate group, it can be determined that the kinetic energy has enhanced benzene sulfonate The role of the level of blood pressure lowering effect.
  • the systolic blood pressure of the low-activity and high-dose groups returned to the control group, no statistical difference; amlodipine 2 mg/kg group, amlodipine besylate 2 mg/ The systolic blood pressure of the kg-energy water group also returned to the control level.
  • DBP diastolic blood pressure
  • the amlodipine 2 mg/kg group After continuous administration for 15 days, compared with the control group, the low- and high-dose kinetic energy group, the amlodipine 2 mg/kg group, the amlodipine besylate 2 mg/kg plus the kinetic energy group had a certain degree of diastolic blood pressure reduction. However, the magnitude of the change was not large, and no statistical difference was found (p>0.05).
  • the diastolic blood pressure of the kinetic energy low group decreased to a certain extent, the decrease was 11.2%, but no statistical difference was found.
  • amlodipine The diastolic blood pressure of the 2 mg/kg group and the amlodipine besylate 2 mg/kg group were significantly decreased (135 ⁇ 23, 148 ⁇ 9, 111 ⁇ 24**, 99 ⁇ 14**, vs 152 ⁇ 10, mmHg, **p ⁇ 0.01).
  • the diastolic blood pressure of the low-activity and high-dose groups returned to the control group, no statistical difference; amlodipine 2 mg/kg group, amlodipine besylate 2 mg/ The systolic blood pressure of the kg-energy water group also returned to the control level.
  • the mean pressure of the kinetic energy low and high dose groups returned to the control group, no statistical difference; amlodipine 2 mg/kg group, amlodipine besylate 2 mg
  • the systolic blood pressure of the /kg plus energy group also returned to the level of the control group.
  • the mean pressure of the amlodipine besylate 2mg/kg plus kinetic energy group increased compared with the control group, with statistical difference (174 ⁇ 13*, vs 161 ⁇ 13, mmHg, *p ⁇ 0.05), it is concluded that this phenomenon is related to the continuous blood pressure reduction, and the physiological rebound of blood pressure after stopping the drug.
  • the heart rate of the low-dose kinetic energy group and the amlodipine besylate 2 mg/kg plus kinetic energy group was not statistically different from the control group.
  • the high-dose group of kinetic energy and benzenesulfonate The heart rate of the amlodipine 2 mg/kg group increased, with statistical difference (438 ⁇ 60*, 439 ⁇ 44**, vs 361 ⁇ 67, times/min, *p ⁇ 0.05, **p ⁇ 0.01).
  • kinetic energy was continuously administered for 15 days and administered for 36 days. Compared with the control group, the kinetic energy was low, the high dose group, the amlodipine 2 mg/kg group, and the amlodipine besylate 2 mg/ The weight of the kinetic energy of the kg-energy water group is relatively uniform.
  • Example 10 Effect of multi-polar micro-kinetic energy drinking water of the present invention on blood pressure of normal SD rats
  • the multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;
  • the positive drug hypotension pill was produced by Beijing Tongrentang Technology Development Co., Ltd. Pharmaceutical Factory, batch number 12072918.
  • Feeding conditions Experimental animal facilities continue to maintain barrier environmental standards.
  • the control range of the main environmental indicators room temperature 20 ⁇ 26 ° C, the daily temperature difference ⁇ 4 ° C.
  • the relative humidity is 40 to 70%.
  • Minimum air exchange times 15 times / hour, light illumination: dark 12h: 12h.
  • the animals are housed in a polypropylene rat group box.
  • the cage specifications are: 545*395*200mm 3 , 5 per box, and the feeding space meets the minimum space required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Provisions. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.
  • Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.
  • Sample collection The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).
  • Test indicators animal general observation, weight, growth status, blood pressure, heart rate.
  • Blood pressure measurement the first week of administration (day 4 of administration), the second week of administration (day 11 of administration), the third week of administration (day 18 of administration), and the fourth week of administration (administration) Day 25)
  • Body weight measurement pre-drug, first week of administration (day 8 of administration), second week of administration (day 15 of administration), third week of administration (day 22 of administration), fourth week of administration (Day 29 of administration)
  • the weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: Kolmogorov-Smirnov method should be used for normal test, Levene median method for homogeneity test of variance, if P>0.05, one-way ANOVA method, if normal and variance The homogeneity test failed (P ⁇ 0.05), then a non-parametric Mann-Whitney test was required.
  • the blood pressure of the experimental group was significantly lower than that of the control group, the difference was significant, and there was no effect on the heart rate of the experimental group, and the experimental results of the auxiliary blood pressure lowering functional animals were determined to be positive.
  • the first week, the second week, the third week, and the fourth week of administration compared with the control group, the systolic blood pressure of the rats with low kinetic energy and high dose group was not statistically compared with the control group. Learning differences, the absolute value did not change much (p>0.05). Compared with the control group, there was no statistical difference in systolic blood pressure between the rats in the antihypertensive group, and the absolute value of the absolute value did not change much (p>0.05).
  • the diastolic blood pressure of the rats with low kinetic energy and high dose group was not statistically compared with the control group. Learning differences, the absolute value did not change much (p>0.05). Compared with the control group, there was no statistical difference in diastolic blood pressure between the rats in the antihypertensive group, and the absolute value of the absolute value did not change much (p>0.05).
  • the third week and the fourth week of administration compared with the control group, the body weight of the low dose group of kinetic energy increased, which was statistically different from the control group (third week: 254 ⁇ 9*, vs 241 ⁇ 14; fourth week: 269 ⁇ 6**, vs 252 ⁇ 17, g, *p ⁇ 0.05, **p ⁇ 0.01).

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Abstract

Uses of a multipolar microkinetic drinking water in preparing a drink, healthcare or medicament used for reducing blood pressure. The multipolar microkinetic drinking water is prepared from raw drinking water being passed through an electromagnetic wave without contact. Compared with the raw drinking water, the ultraviolet absorption peak is offset by 25 nm to 40 nm in the direction of shortwaves, where the preferred offset is 28nm to 30 nm in the direction of the shortwaves, and the optimal offset is either 30 nm or 28 nm in the direction of the shortwaves.

Description

多极微动能饮用水在制备用于降血压的饮料、保健品或药物中的用途Use of multi-pole micro-kinetic drinking water in the preparation of beverages, health products or medicines for lowering blood pressure 技术领域Technical field
本发明属于水处理技术领域，具体涉及多极微动能饮用水在制备用于降血压的饮料、保健品或药物中的用途。The invention belongs to the technical field of water treatment, and particularly relates to the use of multi-pole micro-kinetic energy drinking water in preparing beverages, health care products or medicines for lowering blood pressure.
背景技术Background technique
水(H2O)是由氢、氧两种元素组成的无机物，在常温常压下为无色无味的透明液体。水是最常见的物质之一，是包括人类在内所有生命生存的重要资源，也是生物体最重要的组成部分。水在生命演化中起到了重要的作用。人类很早就开始对水产生了认识，东西方古代朴素的物质观中都把水视为一种基本的组成元素。Water (H 2 O) is an inorganic substance composed of two elements of hydrogen and oxygen, and is a colorless and odorless transparent liquid under normal temperature and normal pressure. Water is one of the most common substances, an important resource for all life, including human beings, and the most important component of living things. Water plays an important role in the evolution of life. Humans have long begun to understand water, and water is regarded as a basic component in the ancient and simple material view of the East and West.
现代人以科学的角度对水有如下认识：Modern people have the following understanding of water from a scientific perspective:
水分子具有记忆力：当科学家将个别具有不同形状和重量的雪花晶体分别溶化而后再冻结起来时，发现重新冻结的雪花晶体的形状和重量与溶化前完全一样，得出水本身有“记忆力”的结论。Water molecules have memory: When scientists melted individual snow crystals with different shapes and weights and then frozen them, it was found that the shape and weight of the re-frozen snow crystals were exactly the same as before melting, which concluded that the water itself had "memory". .
由于水分子本身有其特殊的结构形状，它是由两个氢和一个氧离子以104.5的角度结合而成。它是一种带有电子“磁极性”的离子化系统。水分子在零度及零度以下呈现固态的结晶体，我们称之为冰。然而，水在零至60℃的温度下并不是完全呈现液状，而是以一种液态晶体的形式存在。但如果温度高于60℃，这液态晶体分子便会遭受破坏。Since the water molecule itself has its special structural shape, it is composed of two hydrogen and one oxygen ion combined at an angle of 104.5. It is an ionization system with an electronic "magnetic polarity". Water molecules exhibit solid crystals below zero and below zero, which we call ice. However, water is not completely liquid at a temperature of from zero to 60 ° C, but exists in the form of a liquid crystal. However, if the temperature is higher than 60 ° C, the liquid crystal molecules will be destroyed.
由于水分子的特殊夹角方式，可以将稀释过的溶质分子的形体包含在它的液态晶体中，这就是一般所理解的溶解过程。现在的新发现是，当用某种办法使溶质分子与水分子分离时(如经由多次的稀释与震荡)，水分子仍旧能够保持对溶质分子的“形状”记忆。当机体细胞接触到这样的水分子时，会把这个溶质分子的“形状”记忆当作真的溶质分子一样对待，这是有200多年历史的顺势疗法的基石。Due to the special angle of the water molecules, the form of the diluted solute molecules can be contained in its liquid crystals, which is a generally understood dissolution process. The new discovery is that when a solute molecule is separated from a water molecule by some means (eg, through multiple dilutions and oscillations), the water molecule can still maintain a "shape" memory of the solute molecule. When the body cells are exposed to such water molecules, the "shape" memory of the solute molecule is treated as a true solute molecule, which is the cornerstone of homeopathic therapy with more than 200 years of history.
水由氢与氧两种元素结合而成，但不以单个的水分子存在于自然界，而是由许多个水分子集聚成水分子的集团(H2O)n存在于自然界(即上述的液态结晶)，水分子的集团可能呈链状、环状、团状或葡萄状，最少五个水分子，多则十几、数十至数百个。 Water is composed of two elements of hydrogen and oxygen, but does not exist in nature as a single water molecule. Instead, a group of water molecules (H 2 O) n is present in nature (ie, the above liquid state). Crystallization), the group of water molecules may be chain, ring, agglomerate or grape-like, with at least five water molecules, and more than a dozen, tens to hundreds.
目前已经有采用电磁波来处理水分子，以提高水分子的能量等级或者消毒或者预防和清除污垢的方法。但是这些处理方法均不能很好地控制处理的效果，且处理后的水的效果大多仅限于饮用，没有其他的附加的有利效果。Electromagnetic waves have been used to treat water molecules to increase the energy level of water molecules or to disinfect or prevent and remove dirt. However, these treatment methods do not control the effect of the treatment well, and the effect of the treated water is mostly limited to drinking, and there is no other additional advantageous effect.
发明内容Summary of the invention
为了克服现有技术的缺陷，本发明提供一种采用电磁波非接触式处理得到的多极微动能饮用水及其制备方法和用途。In order to overcome the defects of the prior art, the present invention provides a multi-pole micro-kinetic energy drinking water obtained by electromagnetic wave non-contact treatment, a preparation method thereof and use thereof.
本发明的一个目的是提供一种多极微动能饮用水。本发明的另一个目的是提供一种上述多极微动能饮用水的制备方法。本发明的又一目的是提供一种上述多极微动能饮用水的用途。It is an object of the present invention to provide a multi-pole micro-kinetic energy drinking water. Another object of the present invention is to provide a method of preparing the above-described multi-pole micro-kinetic energy drinking water. It is still another object of the present invention to provide a use of the above-described multi-pole micro-kinetic energy drinking water.
本发明的上述目的是采用如下技术方案来实现的。The above object of the present invention is achieved by the following technical solutions.
一方面，本发明提供一种多极微动能饮用水，该多极微动能饮用水由饮用水原水通过电磁波非接触式处理制备而成，与饮用水原水相比，紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。In one aspect, the present invention provides a multi-pole micro-kinetic energy drinking water prepared by non-contact treatment of drinking water raw water by electromagnetic waves, and the ultraviolet absorption peak is short-waved compared with the raw water of drinking water. The direction shift is 25 nm to 40 nm, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.
与饮用水原水相比，本发明的多极微动能饮用水的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。Compared with the raw water of drinking water, the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
实验表明，本发明的多极微动能饮用水具有更多的核外电子处于高能级非辐射能级态。Experiments have shown that the multi-pole micro-kinetic energy drinking water of the present invention has more extra-nuclear electrons in a high-energy non-radiative energy level.
优选地，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。Preferably, the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Tibet glacier mineral water, Nongfu Spring, Wahaha, and the like.
本发明的多极微动能饮用水在制备好后72小时内饮用或者使用效果最佳。The multi-pole micro-kinetic energy drinking water of the present invention is best consumed or used within 72 hours after preparation.
另一方面，本发明提供一种上述多极微动能饮用水的制备方法，该制备方法包括采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水，其中，低频电磁波的频率范围为30-100kHz，中频电磁波的频率范围为550-720kHz，高频电磁波的频率范围为300-725MHz。In another aspect, the present invention provides a method for preparing the above-mentioned multi-pole micro-kinetic energy drinking water, which comprises non-contact treatment of drinking water raw water by using electromagnetic waves of low, medium and high frequencies, wherein the frequency of low-frequency electromagnetic waves The range is 30-100 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz, and the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
优选地，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。Preferably, the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Tibet glacier mineral water, Nongfu Spring, Wahaha, and the like.
优选地，所述低频电磁波的频率范围为45-75kHz。Preferably, the low frequency electromagnetic wave has a frequency in the range of 45-75 kHz.
优选地，所述中频电磁波的频率范围为600-720kHz。 Preferably, the intermediate frequency electromagnetic wave has a frequency range of 600-720 kHz.
优选地，所述高频电磁波的频率范围为300-425MHz。Preferably, the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.
优选地，所述低频电磁波和中频电磁波为正弦波、方波、尖波、锯齿波或梯形波。Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are a sine wave, a square wave, a sharp wave, a sawtooth wave or a trapezoidal wave.
优选地，所述低频电磁波和中频电磁波波形可以相同或者不同，优选相同。Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform may be the same or different, preferably the same.
优选地，所述低频电磁波和中频电磁波传播方向一致。Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.
优选地，所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。Preferably, the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.
优选地，采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水处理时，先使用低频电磁波和高频电磁波同时处理，然后再使用中频电磁波和高频电磁波同时处理。Preferably, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by the electromagnetic wave non-contact treatment of the drinking water raw water treatment by using three different frequencies of low, medium and high frequencies, and then the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave are simultaneously processed.
优选地，使用低频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟；使用中频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟。Preferably, the time for simultaneous treatment using the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes; the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes. .
优选地，与饮用水原水相比，制备得到的多极微动能饮用水的紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。Preferably, the prepared ultraviolet light absorption peak of the multi-pole micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably to the short-wave direction by 28 nm to 30 nm, and most preferably to the short-wave direction. 30 nm or 28 nm.
与饮用水原水相比，本发明的多极微动能饮用水的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。Compared with the raw water of drinking water, the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .
实验表明，本发明的多极微动能饮用水具有更多的核外电子处于高能级非辐射能级态。Experiments have shown that the multi-pole micro-kinetic energy drinking water of the present invention has more extra-nuclear electrons in a high-energy non-radiative energy level.
在本发明中，产生电磁波的方法及装置是本领域的常规技术手段。In the present invention, a method and apparatus for generating electromagnetic waves are conventional techniques in the art.
又一方面，本发明提供上述多极微动能饮用水在制备各种矿泉水、纯净水、饮料、保健品或药物中的用途。In still another aspect, the present invention provides the use of the above-described multi-pole micro-kinetic energy drinking water in the preparation of various mineral water, purified water, beverages, health care products or medicines.
优选地，所述饮料为功能饮料，可以用于抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和血尿素。Preferably, the beverage is a functional beverage and can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.
优选地，所述保健品或药物可以用于抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和降血尿素。Preferably, the health care product or drug can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid lowering and blood urea lowering.
与现有技术相比，本发明采用电磁波非接触式处理水，没有添加任何添加剂，制作简单，生产效率高，成本低。Compared with the prior art, the invention adopts electromagnetic wave non-contact type treatment water, without adding any additives, is simple to manufacture, has high production efficiency and low cost.
与已有的报道相比较，本发明的多极微动能饮用水较处理前的饮用水 原水在紫外吸收峰向短波方向产生了明显的位移，荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，更多的核外电子处于高能级非辐射能级态。动物实验已经证明具有抗疲劳、减肥、通便、降血压、降血糖、降血尿酸和降血尿素作用。Compared with the existing reports, the multi-pole micro-kinetic energy drinking water of the present invention is more than the drinking water before treatment. The original water produced a significant shift in the ultraviolet absorption peak to the short-wave direction. The spectral intensity of the fluorescent radiation was greatly improved in the 300-400 nm spectrum, and more extranuclear electrons were in the high-energy non-radiative energy level. Animal experiments have proven to have anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea reduction.
附图说明DRAWINGS
以下，结合附图来详细说明本发明的实施方案，其中：Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, in which:
图1为不同处理组的水对过氧化物致DNA损伤的影响；Figure 1 shows the effect of water in different treatment groups on peroxide-induced DNA damage;
图2为不同处理的培养基对细胞的影响；Figure 2 shows the effect of differently treated media on cells;
图3为普通饮用水原水和使用该普通饮用水原水得到的本发明的多极微动能水的吸收光谱分布；3 is an absorption spectrum distribution of the ordinary drinking water raw water and the multi-pole micro kinetic energy of the present invention obtained by using the ordinary drinking water raw water;
图4为依云天然矿泉水(原水)和使用该依云天然矿泉水得到的本发明的多极微动能水的吸收光谱分布；4 is an absorption spectrum distribution of the multi-pole micro kinetic energy of the present invention obtained by Evian natural mineral water (raw water) and using the Evian natural mineral water;
图5为西藏冰川矿泉水(原水)和使用该西藏冰川矿泉水得到的本发明的多极微动能水的吸收光谱分布；5 is an absorption spectrum distribution of the glacial mineral water (raw water) of Tibet and the multi-pole micro kinetic energy of the present invention obtained by using the glacial mineral water of the Tibet;
图6为普通饮用水(原水)与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；Figure 6 is a fluorescence steady state transient test result of ordinary drinking water (raw water) and its multi-pole micro-kinetic energy drinking water;
图7为依云天然矿泉水(原水)和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；Figure 7 is a fluorescence steady state transient test result of Evian natural mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
图8为西藏冰川矿泉水(原水)和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果；Figure 8 shows the fluorescence steady-state transient test results of Tibetan glacial mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;
图9为西藏冰川矿泉水为原水制成的多极微动能饮用水分别取三个样品进行重复性测试结果；Figure 9 shows the results of repeatability test of three samples of multi-pole micro-kinetic energy drinking water made from raw water of Tibet glacial mineral water;
图10为本发明的多极微动能饮用水对SHR自发高血压大鼠血压收缩压(SBP)的影响；Figure 10 is a graph showing the effect of multipolar kinetic energy drinking water on blood pressure systolic blood pressure (SBP) in SHR spontaneously hypertensive rats;
图11为本发明的多极微动能饮用水对SHR自发高血压大鼠血压舒张压(DBP)的影响；Figure 11 is a graph showing the effect of multipolar kinetic energy drinking water on blood pressure diastolic blood pressure (DBP) in SHR spontaneously hypertensive rats;
图12为本发明的多极微动能饮用水对SHR自发高血压大鼠血压平均(MBP)的影响；Figure 12 is a graph showing the effect of multipolar kinetic energy drinking water on the mean blood pressure (MBP) of SHR spontaneously hypertensive rats;
图13为本发明的多极微动能饮用水对SHR自发高血压大鼠心率(HR)的影响；Figure 13 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on heart rate (HR) in SHR spontaneously hypertensive rats;
图14为本发明的多极微动能饮用水对SHR自发高血压大鼠体重的影 响；Figure 14 is a graph showing the effect of multi-polar micro-kinetic energy drinking water on the body weight of SHR spontaneously hypertensive rats ring;
图15动能水对正常SD大鼠血压收缩压(SBP)的影响；Figure 15 Effect of kinetic energy on blood pressure systolic blood pressure (SBP) in normal SD rats;
图16动能水对正常SD大鼠血压舒张压(DBP)的影响；Figure 16 Effect of kinetic energy on blood pressure diastolic blood pressure (DBP) in normal SD rats;
图17动能水对正常SD大鼠血压平均压(MBP)的影响；Figure 17 Effect of kinetic energy on mean blood pressure (MBP) in normal SD rats;
图18动能水对正常SD大鼠心率的影响；Figure 18 Effect of kinetic energy on heart rate in normal SD rats;
图19动能水对正常大鼠血压的影响试验体重变化。Figure 19 Effect of kinetic energy on blood pressure in normal rats Test body weight changes.
具体实施方式detailed description
下面结合具体实施方式对本发明进行进一步的详细描述，给出的实施例仅为了阐明本发明，而不是为了限制本发明的范围。The present invention is further described in detail with reference to the preferred embodiments thereof.
下述实施例中的实验方法，如无特殊说明，均为常规方法。下述实施例中所用的药材原料、试剂材料等，如无特殊说明，均为市售购买产品。The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal materials, reagent materials and the like used in the following examples are commercially available products unless otherwise specified.
实施例1本发明的多极微动能饮用水的制备方法Example 1 Preparation method of multi-pole micro-kinetic energy drinking water of the invention
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为30kHz，中频电磁波的频率范围为550kHz，高频电磁波的频率范围为300MHz。In addition to ordinary drinking water raw water pipes, electromagnetic waves of three different frequencies of low, medium and high are applied in a non-contact manner, wherein the frequency of the low frequency electromagnetic wave is 30 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550 kHz, and the frequency range of the high frequency electromagnetic wave is 300 MHz. .
其中，所述低频电磁波和中频电磁波均为正弦波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理20分钟，然后再使用中频电磁波和高频电磁波同时处理20分钟。处理完成后，得到本发明的多极微动能饮用水。Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both sinusoidal waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.
实施例2本发明的多极微动能饮用水的制备方法Example 2 Preparation method of multi-pole micro-kinetic energy drinking water of the invention
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为100kHz，中频电磁波的频率范围为720kHz，高频电磁波的频率范围为725MHz。In addition to ordinary drinking water raw water pipes, non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 100 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 725 MHz. .
其中，所述低频电磁波和中频电磁波均为方波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理10分钟，然后再使用中频电磁波和高频电磁波同时处理10分钟。处理完成后，得到本发明的多极微动能饮用水。 Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 10 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 10 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.
实施例3本发明的多极微动能饮用水的制备方法Example 3 Preparation method of multi-pole micro-kinetic energy drinking water of the invention
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为45kHz，中频电磁波的频率范围为600kHz，高频电磁波的频率范围为300MHz。In addition to ordinary drinking water raw water pipes, electromagnetic waves of low, medium and high frequencies are applied non-contactly, wherein the frequency of the low frequency electromagnetic waves is 45 kHz, the frequency range of the intermediate frequency electromagnetic waves is 600 kHz, and the frequency range of the high frequency electromagnetic waves is 300 MHz. .
其中，所述低频电磁波和中频电磁波均为方波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理15分钟，然后再使用中频电磁波和高频电磁波同时处理15分钟。处理完成后，得到本发明的多极微动能饮用水。Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 15 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 15 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.
实施例4本发明的多极微动能饮用水的制备方法Example 4 Preparation method of multi-pole micro-kinetic energy drinking water of the invention
在普通饮用水原水管外，非接触式施加低、中、高三种不同频率的电磁波进行处理，其中，低频电磁波的频率为75kHz，中频电磁波的频率范围为720kHz，高频电磁波的频率范围为425MHz。In addition to ordinary drinking water raw water pipes, non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 75 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 425 MHz. .
其中，所述低频电磁波和中频电磁波均为尖波，且二者电磁波传播方向一致。所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。处理时，先使用低频电磁波和高频电磁波同时处理20分钟，然后再使用中频电磁波和高频电磁波同时处理20分钟。处理完成后，得到本发明的多极微动能饮用水。Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sharp waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.
实施例5本发明的多极微动能饮用水的理化指标检测Example 5 Physical and chemical index detection of multi-pole micro-kinetic energy drinking water of the present invention
按照《生活饮用水标准检验方法》(GB/T 5750-2006)对实施例1-4制备的多极微动能饮用水进行检测，检测结果基本一致，结果见表1所示。The multi-pole micro-kinetic energy drinking water prepared in Examples 1-4 was tested according to the Standard Test Method for Drinking Water (GB/T 5750-2006), and the test results were basically the same. The results are shown in Table 1.
表1 本发明的多极微动能饮用水的理化指标检测结果Table 1 Physical and chemical indicators test results of multi-pole micro-kinetic energy drinking water of the present invention
Figure PCTCN2015074761-appb-000001
Figure PCTCN2015074761-appb-000001
Figure PCTCN2015074761-appb-000002
Figure PCTCN2015074761-appb-000002
根据上表可知，本发明的微动能饮用水的指标均符合《生活饮用水卫生标准》(GB5749-2006)的要求。According to the above table, the indicators of the micro-kinetic energy drinking water of the present invention all meet the requirements of the Sanitary Standard for Drinking Water (GB5749-2006).
实施例6本发明的多极微动能饮用水的毒理实验Example 6 Toxicological experiment of multi-pole micro-kinetic energy drinking water of the present invention
一、急性经口毒性试验I. Acute oral toxicity test
1、材料和方法 1. Materials and methods
1.1 受试物：实施例3制备的微动能饮用水1.2动物：由中国食品药品检定研究院实验动物资源研究所提供昆明种小白鼠(合格证号：SCXK(京)2009-0017)，SPF级，体重18.0～22.0g，试验前，动物禁食过夜，自由饮水。1.1 Test substance: Micro-kinetic energy drinking water prepared in Example 3 1.2 Animals: Kunming species of white mice were provided by the Laboratory Animal Resources Research Institute of China Food and Drug Control Research Institute (Qualification No.: SCXK (Beijing) 2009-0017), SPF Grade, body weight 18.0 ~ 22.0g, before the test, the animals fasted overnight, free to drink water.
1.3 剂量分组：受试物设剂量为20000mg/kg体重，雌、雄性动物各10只。按性别分笼群饲。采用一次经口灌胃方式，按0.1ml/10g体重计算染毒量。1.3 Dose grouping: The test substance was set at a dose of 20000 mg/kg body weight, and 10 female and male animals. Grouped by sex. The dose was calculated by oral gavage at 0.1 ml/10 g body weight.
1.4 观察指标：染毒后，观察动物的一般状况、中毒体征和死亡情况，观察期限两周。试验结束时所有动物进行解剖，记录动物的大体病理改变。根据急性毒性分级标准进行急性毒性分级。1.4 Observation indicators: After the exposure, observe the general condition, poisoning signs and death of the animals, and observe the period of two weeks. At the end of the experiment, all animals were dissected and gross pathological changes in the animals were recorded. Acute toxicity grading according to acute toxicity grading standards.
2、试验结果2, test results
受试物对小鼠急性经口毒性试验结果Acute oral toxicity test results of test subjects on mice
Figure PCTCN2015074761-appb-000003
Figure PCTCN2015074761-appb-000003
3、结论3. Conclusion
染毒后，动物无任何中毒症状及死亡情况出现，未见任何大体病理改变，动物体重呈增加趋势。因此，该受试物对雌、雄小白鼠经口LD50均大于10000mg/kg体重，属于实际无毒级。After the exposure, the animals did not show any symptoms of poisoning and death. No gross pathological changes were observed, and the body weight of the animals showed an increasing trend. Therefore, the oral LD 50 of the test article for both female and male mice is greater than 10000 mg/kg body weight, which is an actual non-toxic grade.
二、对过氧化物致DNA损伤影响Second, the effect of peroxide-induced DNA damage
1、实验材料与设备1. Experimental materials and equipment
1.1 5mM维生素C溶液：溶于三蒸水，现用现配，维生素C粉末由Sigma-Aldrich公司提供；1.1 5mM vitamin C solution: dissolved in three distilled water, ready to use, vitamin C powder provided by Sigma-Aldrich;
1.2 5mM CuCl2溶液：溶于三蒸水，现用现配，CuCl2粉末由国药集团化学试剂有限公司提供；1.2 5mM CuCl 2 solution: dissolved in three distilled water, ready to use, CuCl 2 powder is provided by Sinopharm Chemical Reagent Co., Ltd.;
1.3 质粒DNA：pET28a质粒在DH5a大肠杆菌中扩增提取(质粒提取试剂盒购自Promega公司)；1.3 Plasmid DNA: pET28a plasmid was amplified and extracted in DH5a E. coli (plasmid extraction kit was purchased from Promega);
1.4 5mMEDTA溶液pH8.0：EDTA由国药集团化学试剂有限公司提供；1.4 5mM EDTA solution pH 8.0: EDTA is provided by Sinopharm Chemical Reagent Co., Ltd.;
1.5 10×PBS缓冲液：80gNaCl、2gKCl、14.4gNa2HPO4、2.4gKH2PO4 溶解于蒸馏水，调pH值至7.4并定容至1L高压灭菌，所需试剂均购自国药集团化学试剂有限公司；1.5 10×PBS buffer: 80g NaCl, 2gKCl, 14.4g Na 2 HPO 4 , 2.4g KH 2 PO 4 dissolved in distilled water, adjusted to pH 7.4 and fixed to 1L autoclave, the required reagents were purchased from Sinopharm Chemical Reagent Limited company;
1.6 琼脂糖：由Biowest公司提供；1.6 Agarose: supplied by Biowest;
1.7 Goldview核酸染料由Biotium公司提供；1.7 Goldview nucleic acid dyes are supplied by Biotium;
1.8 凝聚成像仪：由赛智创业科技有限公司提供；1.8 Condensation Imager: provided by Saizhi Entrepreneur Technology Co., Ltd.;
1.9 电泳仪：六一仪器厂；1.9 Electrophoresis instrument: Liuyi Instrument Factory;
1.10 受试物：实施例3和4制备的微动能饮用水1.10 Test substance: Micro-kinetic energy drinking water prepared in Examples 3 and 4
2、实验方法2, experimental methods
2.1 实验分组2.1 Experimental grouping
I组：未处理组，采用未处理无菌水加入反应体系；Group I: untreated group, adding untreated sterile water to the reaction system;
II组：实施例3制备的微动能饮用水；Group II: the kinetic energy drinking water prepared in Example 3;
III组：实施例4制备的微动能饮用水。Group III: The kinetic energy drinking water prepared in Example 4.
按照下列顺序加入各反应物质，反应体系终体积为10μL：The reaction materials were added in the following order, and the final volume of the reaction system was 10 μL:
10×PBS缓冲液1μL；10×PBS buffer 1 μL;
质粒DNA(2μg)2μL；Plasmid DNA (2 μg) 2 μL;
5mM维生素C 1μL；5 mM vitamin C 1 μL;
5mM CuC121μL；5 mM CuC1 2 1 μL;
用未处理水或处理水补齐至10μL。Add up to 10 μL with untreated or treated water.
2.2 分别于0.5小时和l小时，加入lμL 5mM EDTA和2.2μL上样缓冲液终止反应；2.2 The reaction was stopped by adding 1 μL of 5 mM EDTA and 2.2 μL of loading buffer at 0.5 hour and 1 hour, respectively.
2.3 0.8％琼脂糠凝胶(含有Goldview染料)电泳l h；2.3 0.8% agar 糠 gel (containing Goldview dye) electrophoresis l h;
2.4 凝胶成像。2.4 Gel imaging.
3、实验结果3. Experimental results
维生素C与二价铜离子发生氧化还原反应，伴随着过氧化物的生成，过氧化物会对质粒DNA造成损伤断裂，体现在电泳条带的减弱。本实验结果如图1所示，实验分为3组，分组方法见方案中描述，每组反应两个时间点:0.5小时和1小时，DNA的亮度代表DNA含量。根据实验结果显示，II组、III组与未处理I组相比较，质粒DNA受氧化还原反应降解的速率基本一致，并没有因为水的处理而产生阻止或者加速DNA损伤断裂的效应。The redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band. The results of this experiment are shown in Figure 1. The experiments were divided into three groups. The grouping method is described in the scheme. The two time points of each group were: 0.5 hours and 1 hour, and the brightness of DNA represented the DNA content. According to the experimental results, the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no effect of preventing or accelerating DNA damage and cleavage due to water treatment.
4、结果分析4, the result analysis
维生素C与二价铜离子发生氧化还原反应，伴随着过氧化物的生成， 过氧化物会对质粒DNA造成损伤断裂，体现在电泳条带的减弱。本实验结果显示，II组、III组与未处理I组相比较，质粒DNA受氧化还原反应降解的速率基本一致，并没有因为水的处理而产业阻止或者加速DNA损伤断裂的效应。说明本发明的多极微动能水对过氧化物致DNA损伤无影响。Vitamin C undergoes redox reaction with divalent copper ions, accompanied by peroxide formation. Peroxide can cause damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band. The results of this experiment showed that the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no industrial effect of preventing or accelerating DNA damage and cleavage due to water treatment. It is indicated that the multipolar micro kinetic energy of the present invention has no effect on peroxide-induced DNA damage.
三、对体外培养HepG2细胞膜完整性影响3. Effect on membrane integrity of HepG2 cells cultured in vitro
1、实验材料与设备1. Experimental materials and equipment
1.1 人肝癌细胞：HepG2；1.1 human liver cancer cells: HepG2;
1.2 细胞培养基：DMEM培养液+10％FBS；1.2 Cell culture medium: DMEM culture solution + 10% FBS;
1.3 FAM-aptamer：购自上海生工生物工程有限公司；1.3 FAM-aptamer: purchased from Shanghai Shenggong Bioengineering Co., Ltd.;
1.4 荧光EZ微镜：OLYMPUS公司；1.4 Fluorescent EZ Micromirror: OLYMPUS;
1.5 96孔细胞培养板；1.5 96-well cell culture plate;
2、实验方法2, experimental methods
2.1 将对数生长期的细胞，加入适量胰酶消化。调整细胞浓度，以4000个细胞/孔的量接种于96孔培养板，过夜培养；2.1 Add the appropriate amount of trypsin to the cells in the logarithmic growth phase. The cell concentration was adjusted, and the cells were seeded in a 96-well culture plate at 4000 cells/well, and cultured overnight;
2.2 将5mL含10％FBS的DMEM培养基(培养基A)采用实施例3的方法处理40分钟(处理组)；2.2 5 mL of DMEM medium (medium A) containing 10% FBS was treated by the method of Example 3 for 40 minutes (treatment group);
2.3 将5mL含10％FBS的DMEM培养基(均养基B)远离实施例3的电磁波源2米以上处理40分钟(未处理组)；2.3 5 mL of DMEM medium (homogeneous B) containing 10% FBS was treated for more than 2 m from the electromagnetic wave source of Example 3 for 40 minutes (untreated group);
2.4 分别将4μg FAM标记的C6-8aptamer加入到100μL培养基A和培养基B中混匀；2.4 Add 4 μg of FAM-labeled C6-8 aptamer to 100 μL of medium A and medium B, respectively;
2.5 将96孔板中贴壁培养的细胞液更换为含有FAM-C6-8aptamer的培养基A和培养基B，37℃培养30min后，用PBS洗一次，荧光显微镜下观察。2.5 The cell culture medium adherently cultured in a 96-well plate was changed to medium A and medium B containing FAM-C6-8 aptamer, cultured at 37 ° C for 30 min, washed once with PBS, and observed under a fluorescence microscope.
3、实验结果3. Experimental results
结果如图2，处理组和未处理组中的细胞形态没有什么区别，都是细胞白发荧光，细胞胞膜完整，细胞内未观察到明显的FAM标记的C6-8aptamer绿色荧光。The results are shown in Fig. 2. There is no difference in cell morphology between the treated group and the untreated group. The cells are white-fluorescent, the cell membrane is intact, and no obvious FAM-labeled C6-8aptamer green fluorescence is observed in the cells.
4、结果与分析4. Results and analysis
如果细胞存在打孔则FAM标记的C6-8aptamer可以进入细胞并与细胞内的蛋白结合，经530波长激发后发绿色荧光。而图中结果看到处理组 和未处理组中的细胞没有什么区别，都是细胞自发荧光，说明细胞胞膜完整，本发明的多极微动能技术对细胞形态及细胞膜完整性无影响。If the cells are punctured, the FAM-labeled C6-8 aptamer can enter the cell and bind to the protein inside the cell, and emit green fluorescence after excitation at 530 wavelength. And the results in the figure see the processing group There is no difference between the cells in the untreated group and the cells, which are autofluorescence, indicating that the cell membrane is intact. The multipolar microkinetic energy technology of the present invention has no effect on cell morphology and cell membrane integrity.
实施例7本发明的多极微动能饮用水的宽光谱吸收率与紫外吸收率Example 7 Broad Spectral Absorption Rate and Ultraviolet Absorption Rate of Multipolar Micro Kinetic Drinking Water of the Present Invention 变化Variety
1、实验材料与设备1. Experimental materials and equipment
1.1 实施例3制备的微动能饮用水；1.1 The kinetic energy drinking water prepared in Example 3;
1.2 实施例3中使用的普通饮用水原水；1.2 ordinary drinking water raw water used in Example 3;
1.3 采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水；1.3 treating the drinking water made of Evian natural mineral water in the same manner as in Example 3;
1.4 依云天然矿泉水：在家乐福超市购买；1.4 Evian natural mineral water: purchased at Carrefour supermarket;
1.5 采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水；1.5 using the same method as in Example 3 to treat drinking water made from Tibetan glacial mineral water;
1.6 西藏冰川矿泉水：在家乐福超市购买；1.6 Tibet Glacier Mineral Water: Purchased at Carrefour Supermarket;
1.7 超纯水：由德国Sartorius超纯水制备系统制得；1.7 Ultrapure water: made by the German Sartorius ultrapure water preparation system;
1.8 光纤光谱仪Ava-Spec3648，测量范围200-1100nm，不确定度/准确度0.5nm；1.8 fiber optic spectrometer Ava-Spec3648, measuring range 200-1100nm, uncertainty / accuracy 0.5nm;
1.9 示波器MS04104，设备出厂编号：C001163，测量精度：1GHz；1.9 oscilloscope MS04104, equipment factory number: C001163, measurement accuracy: 1GHz;
1.10 光电探测器Thorlab DET10A/M，设备出厂编号：JGZX-ZXZC-012，测量精度：200nm-1100nm；1.10 Photodetector Thorlab DET10A/M, equipment ex-factory number: JGZX-ZXZC-012, measurement accuracy: 200nm-1100nm;
1.11 测试条件：温度21℃，湿度：38％。1.11 Test conditions: temperature 21 ° C, humidity: 38%.
2、实验方法2, experimental methods
2.1 宽光谱的吸收率2.1 Wide spectrum absorption rate
(1)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标稳定。(1) Rinse the experimental utensils with about 200 ml of ultrapure water until the physicochemical indexes are stable.
(2)将实施例3中使用的普通饮用水原水和实施例3制备的微动能饮用水置于实验器皿中，扫描200nm～800nm样品标准光源光谱分光的吸收，记录实验结果。(2) The ordinary drinking water raw water used in Example 3 and the micro-kinetic energy drinking water prepared in Example 3 were placed in an experimental vessel, and the absorption of the spectral light spectrum of the standard light source of the sample of 200 nm to 800 nm was scanned, and the experimental results were recorded.
(3)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。(3) Rinse the experimental utensils with about 200 ml of ultrapure water until the physicochemical indexes are restored to their original values and stabilized.
(4)将依云天然矿泉水和采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于实验器皿中，重复步骤(2)，记录期间紫外线强度变化量。(4) Evian natural mineral water and drinking water prepared by treating Evian natural mineral water in the same manner as in Example 3 were placed in an experimental vessel, and step (2) was repeated to record the amount of change in ultraviolet intensity during the recording.
(5)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。 (5) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.
(6)将西藏冰川矿泉水和采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置于实验器皿中，重复步骤(2)，记录期间紫外线强度变化量。(6) The Tibetan glacial mineral water and the drinking water prepared by treating the Tibetan glacial mineral water by the same method as in Example 3 were placed in an experimental vessel, and the step (2) was repeated to record the amount of change in the ultraviolet intensity during the recording.
(7)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。(7) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.
2.2 紫外吸收率测定2.2 Determination of UV absorption rate
(1)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标稳定。(1) Rinse the experimental utensils with about 200 ml of ultrapure water until the physicochemical indexes are stable.
(2)将实施例3制备的微动能饮用水置于紫外线强度探测系统中，紫外光源照射待测水，通过示波器MS04104读取光电探测器Thorlab DET10A/M读取未经过待测水的紫外线强度数值，读取经过待测水的紫外线强度数值，连续不间断测试72小时，记录期间紫外线强度变化量。(2) The micro-kinetic energy prepared in Example 3 is placed in an ultraviolet intensity detecting system, the ultraviolet light source illuminates the water to be tested, and the photodetector Thorlab DET10A/M is read by an oscilloscope MS04104 to read ultraviolet rays that have not passed the water to be tested. The intensity value is read by the value of the ultraviolet intensity of the water to be tested, continuously tested continuously for 72 hours, and the amount of change in ultraviolet intensity during the recording.
(3)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。(3) Rinse the experimental utensils with about 200 ml of ultrapure water until the physicochemical indexes are restored to their original values and stabilized.
(4)将采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于紫外线强度探测系统中，重复步骤(2)，记录期间紫外线强度变化量。(4) The drinking water prepared by the Evian natural mineral water in the same manner as in Example 3 was placed in the ultraviolet intensity detecting system, and the step (2) was repeated to record the amount of change in the ultraviolet intensity during the recording.
(5)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。(5) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.
(6)将采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置于紫外线强度探测系统中，重复步骤(2)，记录期间紫外线强度变化量。(6) The drinking water made from the Tibetan glacial mineral water in the same manner as in Example 3 is placed in the ultraviolet intensity detecting system, and the step (2) is repeated to record the amount of change in the ultraviolet intensity during the recording.
(7)使用约200ml超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。(7) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.
3、实验结果3. Experimental results
3.1 宽光谱吸收率测试3.1 Wide spectrum absorption rate test
结果见图3-5。Results are shown in Figure 3-5.
3.2 紫外吸收率测定3.2 Determination of UV absorption rate
普通水制成的多极微动能饮用水：Multi-pole micro-kinetic energy drinking water made from ordinary water:
紫外吸收率：0.9135->0.9037UV absorption rate: 0.9135->0.9037
依云天然矿泉水制成的多极微动能饮用水：Multi-pole micro-kinetic energy drinking water made from Evian natural mineral water:
紫外吸收率：0.9244->0.9188UV absorption rate: 0.9244->0.9188
西藏冰川矿泉水制成的多极微动能饮用水：Multi-pole micro-kinetic energy drinking water made from Tibetan glacial mineral water:
紫外吸收率：0.9157->0.9079UV absorption rate: 0.9157->0.9079
4、实验结论与分析 4. Experimental conclusions and analysis
通过上述实验，得到如下结论：Through the above experiments, the following conclusions were obtained:
(1)与原水相比较，本发明的多极微动能饮用水的吸收光谱吸收峰向短波偏移30nm左右；(1) Compared with the raw water, the absorption spectrum absorption peak of the multi-pole micro-kinetic energy drinking water of the present invention is shifted to a short wave by about 30 nm;
(2)在72小时的测试下，本发明的多极微动能饮用水的吸收率下降1％。(2) Under the 72-hour test, the absorption rate of the multi-pole micro-kinetic energy drinking water of the present invention decreased by 1%.
实施例8本发明的多极微动能饮用水的受激荧光光谱变化Example 8 Variation of stimulated fluorescence spectrum of multi-pole micro-kinetic energy drinking water of the present invention
1、实验材料与设备1. Experimental materials and equipment
1.1 实施例3制备的微动能饮用水；1.1 The kinetic energy drinking water prepared in Example 3;
1.2 实施例3中使用的普通饮用水原水；1.2 ordinary drinking water raw water used in Example 3;
1.3 采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水；1.3 treating the drinking water made of Evian natural mineral water in the same manner as in Example 3;
1.4 依云天然矿泉水：在家乐福超市购买；1.4 Evian natural mineral water: purchased at Carrefour supermarket;
1.5 采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水；1.5 using the same method as in Example 3 to treat drinking water made from Tibetan glacial mineral water;
1.6 西藏冰川矿泉水：在家乐福超市购买；1.6 Tibet Glacier Mineral Water: Purchased at Carrefour Supermarket;
1.7 超纯水：由德国Sartorius超纯水制备系统制得；1.7 Ultrapure water: made by the German Sartorius ultrapure water preparation system;
1.8 光纤光谱仪Ava-Spec3648，测量范围200-1100nm，不确定度/准确度0.5nm；1.8 fiber optic spectrometer Ava-Spec3648, measuring range 200-1100nm, uncertainty / accuracy 0.5nm;
1.9 测试环境：温度22℃，湿度37％。1.9 Test environment: temperature 22 ° C, humidity 37%.
2、实验方法2, experimental methods
(1)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标稳定。(1) Rinse the experimental utensils with about 200 mL of ultrapure water until the physicochemical indexes are stable.
(2)将实施例3制备的微动能饮用水置于光纤光谱仪样品池中，用266nm脉冲紫外激光器给样品一个激发脉冲，使用光纤光谱仪光谱扫描功能，以对样品的发射光谱进行测量，记录样品的荧光发射光谱。(2) The micro-kinetic energy prepared in Example 3 is placed in a sample cell of a fiber optic spectrometer, and an excitation pulse is given to the sample by a 266 nm pulsed ultraviolet laser, and the spectral spectrum of the sample is used to measure the emission spectrum of the sample. Fluorescence emission spectrum of the sample.
(3)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。(3) Rinse the experimental utensils with about 200 mL of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.
(4)将实施例3中使用的普通饮用水原水置于光纤光谱仪样品池中，重复步骤2，记录实验数据。(4) The ordinary drinking water raw water used in Example 3 was placed in the fiber optic spectrometer sample cell, and step 2 was repeated to record the experimental data.
(5)使用约200mL超纯水冲洗实验用器皿至各项物理化学指标恢复原值并稳定。(5) Rinse the experimental utensils with about 200 mL of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.
(6)将采用采用实施例3的相同的方法处理依云天然矿泉水制成的饮用水置于光谱仪样品池中，重复步骤2，3，记录实验数据。(6) The drinking water prepared by treating Evian natural mineral water in the same manner as in Example 3 was placed in the spectrometer sample cell, and steps 2 and 3 were repeated to record the experimental data.
(7)在将依云天然矿泉水置于光纤光谱仪样品池中，重复步骤2，3记 录实验数据。(7) Repeat the steps 2 and 3 in the EW natural mineral water in the fiber optic spectrometer sample cell. Record experimental data.
(8)将采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水置光纤光谱仪样品池中，重复步骤2，3记录实验数据。(8) The drinking water of the Tibetan glacial mineral water is treated in the sample cell of the optical fiber spectrometer prepared by the same method as in Example 3, and the experimental data is recorded in steps 2 and 3.
(9)将西藏冰川矿泉水置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。(9) Place the Tibetan glacial mineral water in the fiber optic spectrometer sample cell and repeat steps 2 and 3 to record the experimental data.
(10)将采用采用实施例3的相同的方法处理西藏冰川矿泉水制成的饮用水取三个样品分别置于光纤光谱仪样品池中，重复步骤2，3记录实验数据。(10) Three samples of drinking water prepared from the Tibetan glacial mineral water in the same manner as in Example 3 were placed in the sample of the optical fiber spectrometer, and the experimental data were recorded in steps 2 and 3.
3、实验结果3. Experimental results
3.1 荧光稳态瞬态测试3.1 Fluorescence steady state transient test
普通饮用水与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图6。依云天然矿泉水和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图7。西藏冰川矿泉水和与其制成的多极微动能饮用水的荧光稳态瞬态测试结果见图8。西藏冰川矿泉水为原水制成的多极微动能饮用水分别取三个样品进行重复性测试，结果见图9。The fluorescence steady-state transient test results of ordinary drinking water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 6. The fluorescence steady-state transient test results of Evian natural mineral water and its multi-pole micro-kinetic drinking water are shown in Figure 7. The fluorescence steady-state transient test results of Tibetan glacial mineral water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 8. The glacial mineral water in Tibet was tested for repeatability by taking three samples of multi-polar micro-kinetic energy drinking water made of raw water. The results are shown in Figure 9.
4、实验结论与分析4. Experimental conclusions and analysis
由上述实验结果可以看出，与原水相比较，本发明的多极微动能饮用水的三种不同样品的荧光辐射光谱强度在300nm-400nm谱段均有大幅度提升，对同种样品重复实验表明，该实验有很好的重复性。It can be seen from the above experimental results that the fluorescence intensity intensity of the three different samples of the multi-pole micro-kinetic energy drinking water of the present invention is greatly improved in the 300 nm-400 nm spectrum, and is repeated for the same sample. Experiments show that the experiment has good repeatability.
实验表明，本发明的多极微动能水饮用水具有更多的核外电子处于高能级非辐射能级态。Experiments have shown that the multi-pole micro-kinetic water drinking water of the present invention has more extra-nuclear electrons in a high-energy non-radiative energy level.
实施例9本发明的多极微动能饮用水对SHR自发高血压大鼠血压Example 9 Multi-polar micro-kinetic energy drinking water of the present invention on blood pressure of SHR spontaneously hypertensive rats 的影响Impact
1.材料和方法1. Materials and methods
1.1 样品：1.1 Sample:
实施例3制备的多极微动能饮用水(动能水)，无色无味透明水状液体，常温保存，制备好后72h内使用；The multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;
阳性药苯磺酸氨氯地平，由广东彼迪药业有限公司生产，批号20140401。The positive drug amlodipine besylate was produced by Guangdong Pi Di Pharmaceutical Co., Ltd., batch number 20140401.
1.2 实验动物：SHR大鼠，购自北京维通利华生物技术有限公司，生产许可证号：SCXK(京)2012-0001，实验动物质量合格证号： 11400700052484。100只，雌性，订购时56-69日龄，经筛选后选择50只大鼠用于正式试验。1.2 Experimental animals: SHR rats, purchased from Beijing Weitong Lihua Biotechnology Co., Ltd., production license number: SCXK (Beijing) 2012-0001, laboratory animal quality certificate number: 11400700052484. 100 females, 56-69 days old when ordered, 50 rats were selected for formal testing after screening.
饲养条件：实验动物设施持续保持屏障环境标准。主要环境指标的控制范围：室温20～26℃，日温差≤4℃。相对湿度40～70％。最小换气次数15次/小时，光照明：暗＝12h：12h。动物饲养于聚丙烯大鼠群养盒中，笼具规格为：545*395*200mm3，每盒5只，其饲养空间符合中华人民共和国国家标准GB14925-2010中关于实验动物所需最小空间的规定。所有动物均由培训合格的人员进行饲养管理。每周更换1次垫料和笼具。每日添加鼠专用饲料供动物食用，整个饲养过程中保持动物饮食活动自由。Feeding conditions: Experimental animal facilities continue to maintain barrier environmental standards. The control range of the main environmental indicators: room temperature 20 ~ 26 ° C, the daily temperature difference ≤ 4 ° C. The relative humidity is 40 to 70%. Minimum air exchange times 15 times / hour, light illumination: dark = 12h: 12h. The animals are housed in a polypropylene rat group box. The cage specifications are: 545*395*200mm 3 , 5 per box, and the feeding space meets the minimum space required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Provisions. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.
动物福利：本试验所用动物及相关处置应符合动物福利的要求，实验开展要经过中国医学科学院北京协和医学院新药安全评价研究中心动物管理和使用委员会(IACUC)的审查批准。具体注意事项如下：Animal welfare: The animals used in this experiment and related disposals should meet the requirements of animal welfare. The experimental development should be reviewed and approved by the Animal Management and Use Committee (IACUC) of the New Drug Safety Evaluation Research Center of Peking Union Medical College, Chinese Academy of Medical Sciences. The specific considerations are as follows:
(1)实验过程中，应将动物的惊恐和疼痛减少到最低程度。(1) Animal panic and pain should be minimized during the experiment.
(2)在对实验动物进行手术、解剖时，必须进行有效的麻醉。术后恢复期应根据实际情况，进行镇痛和有针对性的护理及饮食调理。(2) Effective anesthesia must be performed when performing and dissecting experimental animals. Postoperative recovery period should be based on actual conditions, analgesia and targeted care and diet conditioning.
(3)保定实验动物时，应避免引起动物的不安、惊恐、疼痛和损伤。保定器具应结构合理、规格适宜、坚固耐用、环保卫生、便于操作。在不影响实验的前提下，对动物身体的强制性限制宜减少到最低程度。(3) When Baoding experimental animals, avoid causing uneasiness, panic, pain and damage to the animals. Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.
(4)样本采集：以安全人道的方式(尽量减少对动物造成的紧张与不适)对实验动物进行采集样本的操作。(4) Sample collection: The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).
(5)对于濒死动物、患病动物以及毒性反应严重的动物，及时报告给兽医及专题负责人，给予及时治疗或实施安乐死。在不影响实验结果判定的情况下，选择“仁慈终点”，避免延长动物承受痛苦的时间。处死实验动物时，按照人道主义原则实施安死术。处死现场，无其他动物在场。确认动物死亡后，妥善处置尸体。(5) For sudden death, sick animals and animals with serious toxic reactions, report to the veterinarian and the person in charge of the case in time for timely treatment or euthanasia. In the case of not affecting the judgment of the experimental results, choose "benevolent end point" to avoid prolonging the time the animal suffers. When the experimental animals were sacrificed, euthanasia was carried out in accordance with humanitarian principles. At the scene of the execution, no other animals were present. After confirming the death of the animal, properly dispose of the body.
(6)职业安全方面：实验过程中，注意采取个人防护措施，如手套、隔离衣、面罩、眼罩、耳塞等。(6) Occupational safety: During the experiment, take personal protective measures such as gloves, gowns, masks, eye masks, earplugs, etc.
1.4 主要仪器与试剂：BP-98A智能无创血压计。1.4 Main instruments and reagents: BP-98A intelligent non-invasive sphygmomanometer.
1.5 试验方法：1.5 Test method:
1.5.1 遵循的标准：辅助降血压功能检验方法，保健食品检验与评价技术规范(2003版)；国食药监保化[2012]107号。1.5.1 Standards to be followed: Auxiliary blood pressure lowering function test method, health food inspection and evaluation technical specification (2003 edition); National Food and Drug Administration [2012] No. 107.
1.5.2 检测指标：动物一般情况观察、体重、生长状况、血压、心率。 1.5.2 Test indicators: animal general observation, weight, growth status, blood pressure, heart rate.
1.5.3 动物分组与剂量设计：动物接收后适应检疫2周，检疫结束时选取50只动物根据血压测定结果进行随机分层分组，共分为5组，每组10只。空白对照组每日饮用正常饮用水；动能水低剂量组每日饮用动能水(饮水瓶灌装动能水，每日补充1次)；动能水高剂量组除每日基础饮用动能水外，每天灌胃动能水一次，剂量为1.5mL/100g；阳性药苯磺酸氨氯地平组每日灌胃一次，给药剂量为2mg/kg，给药体积为1.5mL/kg(苯磺酸氨氯地平成人每日用量10mg，人体重按照60kg计算，为0.167mg/kg，2mg/kg约为人用剂量的12倍，用0.5％CMC-Na配制)；动能水加苯磺酸氨氯地平组苯磺酸氨氯地平剂量也为2mg/kg，用动能水配制。1.5.3 Animal grouping and dose design: Animals were quarantined for 2 weeks after receiving. At the end of quarantine, 50 animals were randomly divided into groups according to the blood pressure measurement results, which were divided into 5 groups, 10 in each group. The blank control group drank normal drinking water daily; the kinetic energy low dose group consumed kinetic energy daily (the drinking bottle filled kinetic energy, supplemented once a day); the kinetic energy high dose group except daily basic drinking kinetic energy, daily The kinetic energy of the broth was once administered at a dose of 1.5 mL/100 g. The positive drug amlodipine besylate was administered once a day at a dose of 2 mg/kg and the dose was 1.5 mL/kg (ammonia chloride benzenesulfonate). The daily dose of adult is 10mg, the human body weight is calculated according to 60kg, which is 0.167mg/kg, 2mg/kg is about 12 times of human dose, prepared with 0.5% CMC-Na); kinetic energy plus amlodipine besylate group The dose of amlodipine besylate was also 2 mg/kg, which was prepared with kinetic energy.
表2 动能水对SHR自发高血压大鼠血压的影响剂量设计Table 2 Effect of kinetic energy on blood pressure of SHR spontaneously hypertensive rats
Figure PCTCN2015074761-appb-000004
Figure PCTCN2015074761-appb-000004
1.5.4 给药时间：连续给药40天。1.5.4 Dosing time: continuous administration for 40 days.
1.5.5 测定时间：1.5.5 Measurement time:
分组前、给药第15天，给药第36天，停止给药后2周，共测定4次。Before the grouping, on the 15th day of administration, the 36th day of administration, and 2 weeks after the administration was stopped, the total was measured 4 times.
1.6 试验数据统计：1.6 Test data statistics:
运用SPSS统计软件处理体重结果并进行给药组与对照组的比较分析。按照以下方法统计：应采用Kolmogorov-Smirnov法进行正态检验，Levene中位数法进行方差齐性检验，若P＞0.05，进行单因素方差分析(One-Way ANOVA)方法，如果正态和方差齐性检验失败(P≤0.05)，那么需要进行非参数Mann-Whitney检验。The weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: Kolmogorov-Smirnov method should be used for normal test, Levene median method for homogeneity test of variance, if P>0.05, one-way ANOVA method, if normal and variance The homogeneity test failed (P ≤ 0.05), then a non-parametric Mann-Whitney test was required.
1.7 结果判定： 1.7 Determination of results:
结果判定：苯磺酸氨氯地平组动物血压明显低于空白对照组，差异具有显著性，表明SHR大鼠模型成功；如果动能水组动物血压明显低于空白对照组，差异具有显著性，对动物心率无影响，可判定动能水具有辅助降血压功能；如果动能水组苯磺酸氨氯地平组血压降低低于苯磺酸氨氯地平组，则可判定动能水具有增强苯磺酸氨氯地平降血压效果的作用。The results showed that the blood pressure of the amlodipine besylate group was significantly lower than that of the blank control group, and the difference was significant, indicating that the SHR rat model was successful. If the blood pressure of the kinetic energy group was significantly lower than that of the blank control group, the difference was significant. The heart rate of the animal has no effect, and it can be judged that the kinetic energy has the function of assisting blood pressure lowering; if the blood pressure of the amlodipine besylate group is lower than that of the amlodipine besylate group, it can be determined that the kinetic energy has enhanced benzene sulfonate The role of the level of blood pressure lowering effect.
2.结果：2. Results:
动物一般情况观察各组均未见异常。Animals were generally observed to have no abnormalities in each group.
表3 动能水对SHR自发高血压大鼠血压收缩压(SBP)的影响(mmHg，
Figure PCTCN2015074761-appb-000005
n＝10)Table 3 Effect of kinetic energy on blood pressure systolic blood pressure (SBP) in SHR spontaneously hypertensive rats (mmHg,
Figure PCTCN2015074761-appb-000005
n=10)
Figure PCTCN2015074761-appb-000006
Figure PCTCN2015074761-appb-000006
注：**，与对照比较，p<0.01Note: **, compared with the control, p<0.01
由表3、图10可见，药前各组别大鼠收缩压(SBP)结果较一致，均未见统计学差异。It can be seen from Table 3 and Figure 10 that the systolic blood pressure (SBP) results of the rats in each group were consistent, and no statistical difference was found.
连续给药15天，与对照组比较，动能水低、高剂量组，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组各组别收缩压数值有一定程度的降低，但变化幅度不大，未见统计学差异(p>0.05)。Continuous administration for 15 days, compared with the control group, the kinetic energy low, high dose group, amlodipine 2mg/kg group, amlodipine besylate 2mg/kg plus kinetic energy group, the systolic blood pressure values of each group have a certain degree Decrease, but the change was not large, and no statistical difference was found (p>0.05).
连续给药36天，与对照组比较，动能水低、高剂量组，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组大鼠收缩压均明显降低(188±18**、193±10**、156±19**、155±12**，vs 216±21，mmHg，**p<0.01)，动能水低剂量组降低幅度为13.0％，动能水高剂量组降低幅度为10.6％。Continuous administration for 36 days, compared with the control group, systolic blood pressure was significantly lower in the low-activity, high-dose group, amlodipine 2 mg/kg group, amlodipine besylate 2 mg/kg plus kinetic energy group (188± 18**, 193±10**, 156±19**, 155±12**, vs 216±21, mmHg, **p<0.01), the kinetic energy low dose group reduced by 13.0%, and the kinetic energy was high. The dose group decreased by 10.6%.
停药后恢复2周，与对照组比较，动能水低、高剂量组的收缩压恢复至对照组水平，未见统计学差异；氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组收缩压也恢复至对照组水平。After stopping the drug for 2 weeks, compared with the control group, the systolic blood pressure of the low-activity and high-dose groups returned to the control group, no statistical difference; amlodipine 2 mg/kg group, amlodipine besylate 2 mg/ The systolic blood pressure of the kg-energy water group also returned to the control level.
表4 动能水对SHR自发高血压大鼠血压舒张压(DBP)的影响(mmHg，
Figure PCTCN2015074761-appb-000007
n＝10) Table 4 Effect of kinetic energy on blood pressure diastolic blood pressure (DBP) in SHR spontaneously hypertensive rats (mmHg,
Figure PCTCN2015074761-appb-000007
n=10)
Figure PCTCN2015074761-appb-000008
Figure PCTCN2015074761-appb-000008
注：*`**，与对照组比较，p<0.05、p<0.01Note: *`**, compared with the control group, p<0.05, p<0.01
由表4、图11可见，药前各组别大鼠舒张压(DBP)结果较一致，均未见统计学差异。It can be seen from Table 4 and Figure 11 that the diastolic blood pressure (DBP) results of the rats in each group were consistent, and no statistical difference was found.
连续给药15天，与对照组比较，动能水低、高剂量组，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组各组别舒张压有一定程度的降低，但变化幅度不大，未见统计学差异(p>0.05)。After continuous administration for 15 days, compared with the control group, the low- and high-dose kinetic energy group, the amlodipine 2 mg/kg group, the amlodipine besylate 2 mg/kg plus the kinetic energy group had a certain degree of diastolic blood pressure reduction. However, the magnitude of the change was not large, and no statistical difference was found (p>0.05).
连续给药36天，与对照组比较，动能水低组大鼠舒张压有一定程度的降低，降低幅度为11.2％，但未见统计学差异，高剂量组未见统计学差异，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg组大鼠舒张压均显著降低(135±23、148±9、111±24**、99±14**，vs 152±10，mmHg，**p<0.01)。After continuous administration for 36 days, compared with the control group, the diastolic blood pressure of the kinetic energy low group decreased to a certain extent, the decrease was 11.2%, but no statistical difference was found. There was no statistical difference in the high dose group, amlodipine The diastolic blood pressure of the 2 mg/kg group and the amlodipine besylate 2 mg/kg group were significantly decreased (135±23, 148±9, 111±24**, 99±14**, vs 152±10, mmHg, **p<0.01).
停药后恢复2周，与对照组比较，动能水低、高剂量组的舒张压恢复至对照组水平，未见统计学差异；氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组收缩压也恢复至对照组水平。此外苯磺酸氨氯地平2mg/kg加动能水组收缩压、平均压与对照组比较有所增加，具有统计学差异(收缩压：154±13*，vs 138±14；平均压：174±13*，vs 161±13，mmHg，*p<0.05)，推断本现象与连续用药明显降压，停药后血压生理性反弹有关。After stopping the drug for 2 weeks, compared with the control group, the diastolic blood pressure of the low-activity and high-dose groups returned to the control group, no statistical difference; amlodipine 2 mg/kg group, amlodipine besylate 2 mg/ The systolic blood pressure of the kg-energy water group also returned to the control level. In addition, the systolic and mean pressure of amlodipine besylate 2mg/kg plus kinetic energy group increased compared with the control group, with statistical difference (systolic blood pressure: 154±13*, vs 138±14; mean pressure: 174± 13*, vs 161±13, mmHg, *p<0.05), it is concluded that this phenomenon is significantly associated with continuous medication, and the physiological rebound of blood pressure after withdrawal.
表5 动能水对SHR自发高血压大鼠血压平均压(MBP)的影响(mmHg，
Figure PCTCN2015074761-appb-000009
n＝10)Table 5 Effect of kinetic energy on mean blood pressure (MBP) of SHR spontaneously hypertensive rats (mmHg,
Figure PCTCN2015074761-appb-000009
n=10)
Figure PCTCN2015074761-appb-000010
Figure PCTCN2015074761-appb-000010
Figure PCTCN2015074761-appb-000011
Figure PCTCN2015074761-appb-000011
注：*`**，与对照组比较，p<0.05、p<0.01Note: *`**, compared with the control group, p<0.05, p<0.01
由表5、图12可见，药前各组别大鼠平均压(MBP)结果较一致，均未见统计学差异。From Table 5 and Figure 12, the mean pressure (MBP) of the rats in each group before the drug was consistent, and no statistical difference was found.
连续给药15天，与对照组比较，动能水低、高剂量组，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组各组别平均压数值有一定程度的降低，但变化幅度不大，未见统计学差异(p>0.05)。Continuous administration for 15 days, compared with the control group, the kinetic energy low, high dose group, amlodipine 2mg/kg group, amlodipine besylate 2mg/kg plus kinetic energy group, the average pressure value of each group has a certain degree Decrease, but the change was not large, and no statistical difference was found (p>0.05).
连续给药36天，与对照组比较，动能水低组大鼠平均压有一定程度的降低，有统计学差异，降低幅度为11.0％，高剂量组未见明显变化，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组大鼠舒平均压均显著降低(153±19*、163±9、126±22**、118±11**，vs 172±11，mmHg，*p<0.05、**p<0.01)。After continuous administration for 36 days, compared with the control group, the mean pressure of the kinetic energy low group decreased to some extent, with a statistical difference, the reduction was 11.0%, no significant change in the high dose group, amlodipine 2mg/kg The average pressure of the rats in the 2 mg/kg amlodipine besylate plus kinetic energy group was significantly decreased (153±19*, 163±9, 126±22**, 118±11**, vs 172±11, mmHg, *p<0.05, **p<0.01).
停药后恢复2周，与对照组比较，动能水低、高剂量组的平均压均恢复至对照组水平，未见统计学差异；氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组收缩压也恢复至对照组水平，此外苯磺酸氨氯地平2mg/kg加动能水组平均压与对照组比较有所增加，具有统计学差异(174±13*，vs 161±13，mmHg，*p<0.05)，推断本现象与连续用药明显降压，停药后血压生理性反弹有关。After stopping the drug for 2 weeks, compared with the control group, the mean pressure of the kinetic energy low and high dose groups returned to the control group, no statistical difference; amlodipine 2 mg/kg group, amlodipine besylate 2 mg The systolic blood pressure of the /kg plus energy group also returned to the level of the control group. In addition, the mean pressure of the amlodipine besylate 2mg/kg plus kinetic energy group increased compared with the control group, with statistical difference (174±13*, vs 161±13, mmHg, *p<0.05), it is concluded that this phenomenon is related to the continuous blood pressure reduction, and the physiological rebound of blood pressure after stopping the drug.
表6 动能水对SHR自发高血压大鼠心率的影响(次/min，
Figure PCTCN2015074761-appb-000012
n＝10)Table 6 Effect of kinetic energy on heart rate in SHR spontaneously hypertensive rats (time/min,
Figure PCTCN2015074761-appb-000012
n=10)
Figure PCTCN2015074761-appb-000013
Figure PCTCN2015074761-appb-000013
注：*`**，与对照组比较，p<0.05、p<0.01Note: *`**, compared with the control group, p<0.05, p<0.01
由表6、图13可见，药前各组别大鼠心率(HR)结果较一致，均未见统计学差异。 It can be seen from Table 6 and Figure 13. The heart rate (HR) of the rats in each group before the drug was consistent, and no statistical difference was found.
连续给药15天，与对照组比较，动能水低、高剂量组心率绝对值有所增加，但未见统计学差异；氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组心率有所增加，有统计学差异(454±44*、475±57*，vs 359±124，次/min，*p<0.05)。Continuous administration for 15 days, compared with the control group, the absolute value of heart rate in the low- and high-dose kinetic energy group increased, but no statistical difference; amlodipine 2mg/kg group, amlodipine besylate 2mg/kg plus The heart rate of the kinetic energy group increased, with statistical differences (454±44*, 475±57*, vs 359±124, times/min, *p<0.05).
连续给药36天，与对照组比较，动能水低、高剂量组，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组心率未见统计学差异。After continuous administration for 36 days, compared with the control group, there was no statistical difference in heart rate between the low-dynamic and high-dose kinetic energy groups, the amlodipine 2 mg/kg group, and the amlodipine besylate 2 mg/kg plus kinetic energy group.
停药后恢复2周，与对照组比较，动能水低剂量组、苯磺酸氨氯地平2mg/kg加动能水组心率与对照组比较未见统计学差异，动能水高剂量组、苯磺酸氨氯地平2mg/kg组心率有所增加，具有统计学差异(438±60*、439±44**，vs 361±67，次/min，*p<0.05、**p<0.01)。After stopping the drug for 2 weeks, compared with the control group, the heart rate of the low-dose kinetic energy group and the amlodipine besylate 2 mg/kg plus kinetic energy group was not statistically different from the control group. The high-dose group of kinetic energy and benzenesulfonate The heart rate of the amlodipine 2 mg/kg group increased, with statistical difference (438±60*, 439±44**, vs 361±67, times/min, *p<0.05, **p<0.01).
表7 动能水对SHR自发高血压大鼠体重的影响(g，
Figure PCTCN2015074761-appb-000014
n＝10)Table 7 Effect of kinetic energy on body weight of SHR spontaneously hypertensive rats (g,
Figure PCTCN2015074761-appb-000014
n=10)
Figure PCTCN2015074761-appb-000015
Figure PCTCN2015074761-appb-000015
由表7、图14可见，动能水连续给药15天、给药36天，与对照组比较，动能水低、高剂量组，氨氯地平2mg/kg组、苯磺酸氨氯地平2mg/kg加动能水组动能水体重较一致。As can be seen from Table 7 and Figure 14, kinetic energy was continuously administered for 15 days and administered for 36 days. Compared with the control group, the kinetic energy was low, the high dose group, the amlodipine 2 mg/kg group, and the amlodipine besylate 2 mg/ The weight of the kinetic energy of the kg-energy water group is relatively uniform.
3.小结3. Summary
综合上述结果，在本实验条件下，连续饮用本发明的多极微动能饮用水36天，可一定程度降低SHR自发高血压大鼠的收缩压和平均压。Based on the above results, under the experimental conditions, continuous drinking of the multi-polar micro-kinetic energy drinking water of the present invention for 36 days can reduce the systolic blood pressure and the average pressure of SHR spontaneously hypertensive rats to some extent.
提示动能水具有降低血压的作用。It is suggested that kinetic energy has the effect of lowering blood pressure.
实施例10本发明的多极微动能饮用水对正常SD大鼠血压的影响Example 10 Effect of multi-polar micro-kinetic energy drinking water of the present invention on blood pressure of normal SD rats
1.材料和方法1. Materials and methods
1.1 样品：1.1 Sample:
实施例3制备的多极微动能饮用水(动能水)，无色无味透明水状液体，常温保存，制备好后72h内使用；The multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;
阳性药降压丸，由北京同仁堂科技发展股份有限公司制药厂生产，批号12072918。 The positive drug hypotension pill was produced by Beijing Tongrentang Technology Development Co., Ltd. Pharmaceutical Factory, batch number 12072918.
1.2 实验动物：SD大鼠，购自北京华阜康生物科技股份有限公司，生产许可证号：SCXK(京)2009-0004，40只，雄性，订购体重160～180g。实验动物质量合格证号：11401300009312。1.2 Experimental animals: SD rats, purchased from Beijing Huakangkang Biotechnology Co., Ltd., production license number: SCXK (Beijing) 2009-0004, 40, male, ordering weight 160-180g. Laboratory animal quality certificate number: 11401300009312.
饲养条件：实验动物设施持续保持屏障环境标准。主要环境指标的控制范围：室温20～26℃，日温差≤4℃。相对湿度40～70％。最小换气次数15次/小时，光照明：暗＝12h：12h。动物饲养于聚丙烯大鼠群养盒中，笼具规格为：545*395*200mm3，每盒5只，其饲养空间符合中华人民共和国国家标准GB14925-2010中关于实验动物所需最小空间的规定。所有动物均由培训合格的人员进行饲养管理。每周更换1次垫料和笼具。每日添加鼠专用饲料供动物食用，整个饲养过程中保持动物饮食活动自由。Feeding conditions: Experimental animal facilities continue to maintain barrier environmental standards. The control range of the main environmental indicators: room temperature 20 ~ 26 ° C, the daily temperature difference ≤ 4 ° C. The relative humidity is 40 to 70%. Minimum air exchange times 15 times / hour, light illumination: dark = 12h: 12h. The animals are housed in a polypropylene rat group box. The cage specifications are: 545*395*200mm 3 , 5 per box, and the feeding space meets the minimum space required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Provisions. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.
动物福利：本试验所用动物及相关处置应符合动物福利的要求，实验开展要经过中国医学科学院北京协和医学院新药安全评价研究中心动物管理和使用委员会(IACUC)的审查批准。具体注意事项如下：Animal welfare: The animals used in this experiment and related disposals should meet the requirements of animal welfare. The experimental development should be reviewed and approved by the Animal Management and Use Committee (IACUC) of the New Drug Safety Evaluation Research Center of Peking Union Medical College, Chinese Academy of Medical Sciences. The specific considerations are as follows:
(1)实验过程中，应将动物的惊恐和疼痛减少到最低程度。(1) Animal panic and pain should be minimized during the experiment.
(2)在对实验动物进行手术、解剖时，必须进行有效的麻醉。术后恢复期应根据实际情况，进行镇痛和有针对性的护理及饮食调理。(2) Effective anesthesia must be performed when performing and dissecting experimental animals. Postoperative recovery period should be based on actual conditions, analgesia and targeted care and diet conditioning.
(3)保定实验动物时，应避免引起动物的不安、惊恐、疼痛和损伤。保定器具应结构合理、规格适宜、坚固耐用、环保卫生、便于操作。在不影响实验的前提下，对动物身体的强制性限制宜减少到最低程度。(3) When Baoding experimental animals, avoid causing uneasiness, panic, pain and damage to the animals. Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.
(4)样本采集：以安全人道的方式(尽量减少对动物造成的紧张与不适)对实验动物进行采集样本的操作。(4) Sample collection: The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).
(5)对于濒死动物、患病动物以及毒性反应严重的动物，及时报告给兽医及专题负责人，给予及时治疗或实施安乐死。在不影响实验结果判定的情况下，选择“仁慈终点”，避免延长动物承受痛苦的时间。处死实验动物时，按照人道主义原则实施安死术。处死现场，无其他动物在场。确认动物死亡后，妥善处置尸体。(5) For sudden death, sick animals and animals with serious toxic reactions, report to the veterinarian and the person in charge of the case in time for timely treatment or euthanasia. In the case of not affecting the judgment of the experimental results, choose "benevolent end point" to avoid prolonging the time the animal suffers. When the experimental animals were sacrificed, euthanasia was carried out in accordance with humanitarian principles. At the scene of the execution, no other animals were present. After confirming the death of the animal, properly dispose of the body.
(6)职业安全方面：实验过程中，注意采取个人防护措施，如手套、隔离衣、面罩、眼罩、耳塞等。(6) Occupational safety: During the experiment, take personal protective measures such as gloves, gowns, masks, eye masks, earplugs, etc.
1.4 主要仪器与试剂：BP-98A智能无创血压计。1.4 Main instruments and reagents: BP-98A intelligent non-invasive sphygmomanometer.
1.5 试验方法：1.5 Test method:
1.5.1 遵循的标准：辅助降血压功能检验方法，保健食品检验与评价技术规范(2003版)；国食药监保化[2012]107号。 1.5.1 Standards to be followed: Auxiliary blood pressure lowering function test method, health food inspection and evaluation technical specification (2003 edition); National Food and Drug Administration [2012] No. 107.
1.5.2 检测指标：动物一般情况观察、体重、生长状况、血压、心率。1.5.2 Test indicators: animal general observation, weight, growth status, blood pressure, heart rate.
1.5.3 动物分组与剂量设计：动物接收后适应检疫3天，检疫结束时选取40只动物根据血压测定结果进行随机分层分组，共分为4组，每组10只。空白对照组每日饮用正常饮用水；动能水低剂量组每日饮用动能水(饮水瓶灌装动能水，每日更换1次)；动能水高剂量组除每日基础饮用动能水外，每天灌胃动能水一次，剂量为1.5mL/100g；降压丸组每日灌胃一次，给药剂量为3g/kg，给药体积为1.5mL/100g(降压丸成人每日用量18g，人体重按照60kg计算，为0.3g/kg，以人用量的10倍计算，折合3g/kg，给药体积为15mL/kg，药物配制浓度为0.2g/mL)。1.5.3 Animal grouping and dose design: Animals were quarantined for 3 days after receiving. At the end of quarantine, 40 animals were randomly divided into groups according to the blood pressure measurement results, which were divided into 4 groups, 10 in each group. The blank control group drank normal drinking water daily; the kinetic energy low dose group consumed kinetic energy daily (the drinking bottle filled kinetic energy, changed once a day); the kinetic energy high dose group except daily basic drinking kinetic energy, daily The kinetic energy of water was administered once, the dose was 1.5mL/100g; the blood pressure pill group was administered once a day, the dose was 3g/kg, and the dosage volume was 1.5mL/100g (the daily dosage of antihypertensive pills was 18g, human The body weight was calculated according to 60 kg, which was 0.3 g/kg, calculated as 10 times the human dose, and was equivalent to 3 g/kg, the administration volume was 15 mL/kg, and the drug preparation concentration was 0.2 g/mL).
表8 动能水对正常动物血压的影响Table 8 Effect of kinetic energy on blood pressure of normal animals
Figure PCTCN2015074761-appb-000016
Figure PCTCN2015074761-appb-000016
1.5.4 给药时间：连续给药32天。1.5.4 Dosing time: continuous administration for 32 days.
1.5.5 测定时间：1.5.5 Measurement time:
血压测定：给药第一周(给药第4天)，给药第二周(给药第11天)，给药第三周(给药第18天)，给药第四周(给药第25天)Blood pressure measurement: the first week of administration (day 4 of administration), the second week of administration (day 11 of administration), the third week of administration (day 18 of administration), and the fourth week of administration (administration) Day 25)
体重测定：药前、给药第一周(给药第8天)，给药第二周(给药第15天)，给药第三周(给药第22天)，给药第四周(给药第29天)Body weight measurement: pre-drug, first week of administration (day 8 of administration), second week of administration (day 15 of administration), third week of administration (day 22 of administration), fourth week of administration (Day 29 of administration)
1.6 试验数据统计：1.6 Test data statistics:
运用SPSS统计软件处理体重结果并进行给药组与对照组的比较分析。按照以下方法统计：应采用Kolmogorov-Smirnov法进行正态检验，Levene中位数法进行方差齐性检验，若P＞0.05，进行单因素方差分析(One-Way ANOVA)方法，如果正态和方差齐性检验失败(P≤0.05)，那么需要进行非参数Mann-Whitney检验。The weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: Kolmogorov-Smirnov method should be used for normal test, Levene median method for homogeneity test of variance, if P>0.05, one-way ANOVA method, if normal and variance The homogeneity test failed (P ≤ 0.05), then a non-parametric Mann-Whitney test was required.
1.7 结果判定1.7 Result determination
结果判定：实验组动物血压明显低于对照组，差异具有显著性，且对实验组动物心率无影响，可判定辅助降血压功能动物实验结果阳性。 The results were determined: the blood pressure of the experimental group was significantly lower than that of the control group, the difference was significant, and there was no effect on the heart rate of the experimental group, and the experimental results of the auxiliary blood pressure lowering functional animals were determined to be positive.
2.结果2. Results
动物一般情况观察各组均未见异常。Animals were generally observed to have no abnormalities in each group.
表9 动能水对正常SD大鼠血压收缩压的影响(SBP，mmHg，
Figure PCTCN2015074761-appb-000017
n＝10)Table 9 Effect of kinetic energy on blood pressure systolic blood pressure in normal SD rats (SBP, mmHg,
Figure PCTCN2015074761-appb-000017
n=10)
Figure PCTCN2015074761-appb-000018
Figure PCTCN2015074761-appb-000018
由表9、图15可见，给药第一周、第二周、第三周、第四周，与对照组比较，动能水低、高剂量组大鼠收缩压与对照组比较均未见统计学差异，绝对值变化不大(p>0.05)。与对照组比较，降压丸组大鼠收缩压未见统计学差异，绝对值绝对值变化不大(p>0.05)。As can be seen from Table 9 and Figure 15, the first week, the second week, the third week, and the fourth week of administration, compared with the control group, the systolic blood pressure of the rats with low kinetic energy and high dose group was not statistically compared with the control group. Learning differences, the absolute value did not change much (p>0.05). Compared with the control group, there was no statistical difference in systolic blood pressure between the rats in the antihypertensive group, and the absolute value of the absolute value did not change much (p>0.05).
表10 动能水对正常SD大鼠血压舒张压的影响(DBP，mmHg，
Figure PCTCN2015074761-appb-000019
n＝10)Table 10 Effect of kinetic energy on blood pressure and diastolic blood pressure in normal SD rats (DBP, mmHg,
Figure PCTCN2015074761-appb-000019
n=10)
Figure PCTCN2015074761-appb-000020
Figure PCTCN2015074761-appb-000020
由表10、图16可见，给药第一周、第二周、第三周、第四周，与对照组比较，动能水低、高剂量组大鼠舒张压与对照组比较均未见统计学差异，绝对值变化不大(p>0.05)。与对照组比较，降压丸组大鼠舒张压未见统计学差异，绝对值绝对值变化不大(p>0.05)。As can be seen from Table 10 and Figure 16, the first week, the second week, the third week, and the fourth week of administration, compared with the control group, the diastolic blood pressure of the rats with low kinetic energy and high dose group was not statistically compared with the control group. Learning differences, the absolute value did not change much (p>0.05). Compared with the control group, there was no statistical difference in diastolic blood pressure between the rats in the antihypertensive group, and the absolute value of the absolute value did not change much (p>0.05).
表11 动能水对正常SD大鼠血压平均压的影响(DBP，mmHg，
Figure PCTCN2015074761-appb-000021
n＝10)Table 11 Effect of kinetic energy on mean blood pressure of normal SD rats (DBP, mmHg,
Figure PCTCN2015074761-appb-000021
n=10)
Figure PCTCN2015074761-appb-000022
Figure PCTCN2015074761-appb-000022
由表11、图17可见，给药第一周、第二周、第三周、第四周，与对照组比较，动能水低、高剂量组大鼠平均压未见统计学差异，绝对值变化不大(p>0.05)。与对照组比较，降压丸组大鼠平均压未见统计学差异，绝对值绝对值变化不大(p>0.05)。As can be seen from Table 11 and Figure 17, the first week, the second week, the third week, and the fourth week of administration, compared with the control group, there was no statistical difference in the mean pressure of the rats with low kinetic energy and high dose, absolute value. The change was not significant (p>0.05). Compared with the control group, there was no statistical difference in the mean pressure of the rats in the antihypertensive group, and the absolute value of the absolute value did not change much (p>0.05).
表12 动能水对正常SD大鼠心率的影响(HR，次/min，
Figure PCTCN2015074761-appb-000023
n＝10)Table 12 Effect of kinetic energy on heart rate in normal SD rats (HR, min/min,
Figure PCTCN2015074761-appb-000023
n=10)
Figure PCTCN2015074761-appb-000024
Figure PCTCN2015074761-appb-000024
由表12、图18可见，给药第一周、第二周、第三周、第四周，与对照组比较，动能水低、高剂量组大鼠心率未见统计学差异，绝对值变化不大(p>0.05)。与对照组比较，降压丸组大鼠心率未见统计学差异，绝对值绝对值变化不大(p>0.05)。As can be seen from Table 12 and Figure 18, the first week, the second week, the third week, and the fourth week of administration, compared with the control group, there was no statistical difference in the heart rate between the low- and high-dose groups of kinetic energy, and the absolute value was changed. Not large (p>0.05). Compared with the control group, there was no statistical difference in heart rate between the rats in the antihypertensive group, and the absolute value of the absolute value did not change much (p>0.05).
表13、动能水对正常SD大鼠体重的影响(g，
Figure PCTCN2015074761-appb-000025
n＝10)Table 13. Effect of kinetic energy on body weight of normal SD rats (g,
Figure PCTCN2015074761-appb-000025
n=10)
Figure PCTCN2015074761-appb-000026
Figure PCTCN2015074761-appb-000026
注：与对照组比较，*p<0.05；**p<0.01Note: compared with the control group, *p<0.05; **p<0.01
由表13、图19可见，给药第三周、第四周，与对照组比较，动能水低剂量组大鼠体重有所增加，与对照组比较有统计学差异(第三周：254±9*，vs 241±14；第四周：269±6**，vs 252±17，g，*p<0.05、**p<0.01)。As can be seen from Table 13 and Figure 19, the third week and the fourth week of administration, compared with the control group, the body weight of the low dose group of kinetic energy increased, which was statistically different from the control group (third week: 254± 9*, vs 241±14; fourth week: 269±6**, vs 252±17, g, *p<0.05, **p<0.01).
3.小结3. Summary
综合上述结果，在本实验条件下，连续饮用本发明的多极微动能饮用Based on the above results, under the experimental conditions, the multi-polar micro-kinetic energy of the present invention is continuously consumed.
水32天，对正常大鼠血压未见明显影响。 Water for 32 days had no significant effect on blood pressure in normal rats.

Claims (10)

  1. 多极微动能饮用水在制备用于降血压的饮料、保健品或药物中的用途。Use of multi-pole micro-kinetic energy drinking water in the preparation of beverages, health care products or medicines for lowering blood pressure.
  2. 根据权利要求1所述的用途，其特征在于，所述多极微动能饮用水采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水制备而成，其中，低频电磁波的频率范围为30-100kHz，中频电磁波的频率范围为550-720kHz，高频电磁波的频率范围为300-725MHz。The use according to claim 1, wherein the multi-pole micro-kinetic energy drinking water is prepared by non-contact treatment of drinking water raw water with low, medium and high electromagnetic waves of different frequencies, wherein the frequency of the low frequency electromagnetic wave The range is 30-100 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz, and the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
  3. 根据权利要求1或2所述的用途，其特征在于，所述饮用水原水为普通自来水或者各种矿泉水或纯净水，例如依云天然矿泉水、西藏冰川矿泉水、农夫山泉、娃哈哈等等。The use according to claim 1 or 2, characterized in that the raw water of drinking water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Tibet glacier mineral water, Nongfu Spring, Wahaha, etc. .
  4. 根据权利要求1至3中任一项所述的用途，其特征在于，所述低频电磁波的频率范围为45-75kHz；The use according to any one of claims 1 to 3, characterized in that the frequency of the low frequency electromagnetic wave is in the range of 45-75 kHz;
    优选地，所述中频电磁波的频率范围为600-720kHz；Preferably, the frequency of the intermediate frequency electromagnetic wave ranges from 600 to 720 kHz;
    优选地，所述高频电磁波的频率范围为300-425MHz。Preferably, the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.
  5. 根据权利要求1至4中任一项所述的用途，其特征在于，所述低频电磁波和中频电磁波为正弦波、方波、尖波、锯齿波或梯形波；The use according to any one of claims 1 to 4, wherein the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sine waves, square waves, sharp waves, sawtooth waves or trapezoidal waves;
    优选地，所述低频电磁波和中频电磁波波形相同或者不同；Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform are the same or different;
    更优选地，所述低频电磁波和中频电磁波波形优选相同。More preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform are preferably the same.
  6. 根据权利要求1至5中任一项所述的用途，其特征在于，所述低频电磁波和中频电磁波传播方向一致。The use according to any one of claims 1 to 5, characterized in that the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.
  7. 根据权利要求1至6中任一项所述的用途，所述高频电磁波为三角波，且与所述低频电磁波和中频电磁波的传播方向垂直。The use according to any one of claims 1 to 6, wherein the high-frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low-frequency electromagnetic wave and the intermediate-frequency electromagnetic wave.
  8. 根据权利要求1至7中任一项所述的用途，其特征在于，采用低、中、高三种不同频率的电磁波非接触式处理饮用水原水处理时，先使用低频电磁波和高频电磁波同时处理，然后再使用中频电磁波和高频电磁波同时处理；The use according to any one of claims 1 to 7, wherein the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by non-contact treatment of drinking water raw water by using three different frequencies of low, medium and high frequencies. And then using intermediate frequency electromagnetic waves and high frequency electromagnetic waves for simultaneous processing;
    优选地，使用低频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟；Preferably, the time for simultaneous treatment using low frequency electromagnetic waves and high frequency electromagnetic waves is 10-30 minutes, preferably 10-20 minutes;
    更优选地，使用中频电磁波和高频电磁波同时处理的时间为10-30分钟，优选为10-20分钟。More preferably, the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10 to 30 minutes, preferably 10 to 20 minutes.
  9. 根据权利要求1至8中任一项所述的用途，其特征在于，与饮用 水原水相比，所述多极微动能饮用水的紫外吸收峰向短波方向偏移25nm-40nm，优选向短波方向偏移28nm-30nm，最优选向短波方向偏移30nm或28nm。Use according to any one of claims 1 to 8, characterized in that it is The ultraviolet absorption peak of the multi-pole micro-kinetic energy drinking water is shifted by 25 nm to 40 nm in the short-wave direction, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.
  10. 根据权利要求1至9中任一项所述的用途，其特征在于，所述饮料为功能饮料，可以用于降血压；The use according to any one of claims 1 to 9, wherein the beverage is a functional beverage and can be used for lowering blood pressure;
    优选地，所述保健品或药物可以用于降血压。 Preferably, the health supplement or drug can be used to lower blood pressure.
PCT/CN2015/074761 2015-03-20 2015-03-20 Uses of multipolar microkinetic drinking water in preparing drink, healthcare or medicament used for reducing blood pressure WO2016149883A1 (en)

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