AU2015101916A4 - Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament for use in reducing blood urea - Google Patents
Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament for use in reducing blood urea Download PDFInfo
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Abstract
Abstract Uses of a multipolar microkinetic drinking water in preparing a drink, healthcare product or medicament for use in reducing blood urea. The multipolar microkinetic drinking water is prepared from raw drinking water being passed through an electromagnetic wave without contact. Compared with the raw drinking water, the ultraviolet absorption peak is offset by 25 nm to 40 nm in the direction of shortwaves, where a preferred offset is between 28 nm and 30 nm, and the optimal offset is either 30 nm or 28 nm.
Description
USES OF MULTIPOLAR MICRO KINETIC DRINKING WATER IN PREPARING DRINK, HEALTHCARE PRODUCT OR MEDICAMENT USED FOR REDUCING BLOOD UREA
TECHNICAL FIELD
The present invention relates to the field of water treatment technology and more particularly to uses of multipolar microkinetic drinking water in the preparation of beverages, health care products or medicaments for reducing blood urea.
BACKGROUND OF THE INVENTION
Water (H2O) is an inorganic substance consisting of two elements of hydrogen and oxygen, and is a colorless, odorless and transparent liquid at normal temperature and pressure. Water is one of the most common substances and it is an important resource for the survival of all life, including human beings and it is also the most important part of the organism. Water plays an important role in the evolution of life. Humans began to generate awareness on the water very early, in the ancient East-West simple material concept, water was regarded as a basic constituent element.
Modem people have the following cognitions on water from a scientific angle:
Water molecules have memory: when scientists melted and then refreezed up individual snow crystals with different shapes and weights, it was found that the shape and weight of the re-frozen snow crystals were exactly the same as that before melting and therefore, scientists come to the conclusion that the water itself has memory.
Since the water molecule itself has its own special structural shape, it is made up of two hydrogens and one oxygen ion at an angle of 104.5. It is a kind of
2015101916 20 Mar 2015 ionization system with electronic magnetic polarity. Water molecule at zero and below exhibits the state of solid crystal, and we call it ice. However water is not completely liquid at a temperature of 0 to 60 °C, but in the form of a liquid crystal. But if the temperature is higher than 60 °C, this liquid crystal molecule will be destroyed.
Due to the special angle of the water molecule, the body of the diluted solute molecule can be contained in its liquid crystal, which is the general understanding of the dissolution process. At present, new finding is that water molecules are still able to keep the shape” memory on the solute molecules when the solute molecules are separated from the water molecules by some means (such as by repeated dilution and shock). When the body cells are exposed to such water molecules, due to the shape memory on the solute molecule they will be treated as true solute molecules, this is the cornerstone of homeopathic therapy for more than 200 years.
Water is composed of two elements of hydrogen and oxygen, it does not exsit in the form of individual water molecules, but in the form of water molecules group (H2O)n (i.e. the liquid crystal described above), which is formed by gathering many water molecules in nature. The water molecules group may be chain-like, ring-like, slug-like or grape-like, and have at least five, or as many as more than ten, dozens to hundreds of water molecules.
There are already some methods for treating water molecules to increase the energy level of water molecules or disinfect or prevent and remove dirts by using electromagnetic waves. However, all of these methods can not adequately control the treatment effect, and the treated water by such methods is mostly used as the drinking water without other additional beneficial effects.
SUMMARY OF THE INVENTION
In order to overcome the drawbacks of the prior art, the present invention provides a multi-polar micro-kinetic energy drinking water, which was obtained
2015101916 20 Mar 2015 by using the electromagnetic wave in non-contact mode and preparation method for same and use thereof.
It is an object of the present invention to provide a multi-polar micro-kinetic energy drinking water. It is another object of the present invention to provide a preparation method of the above multi-polar micro-kinetic energy drinking water. It is yet another object of the present invention to provide a use of the above multi-polar micro-kinetic energy drinking water.
The above objects of the present invention are achieved by the following technical solution.
In one aspect, the present invention provides a multi-polar micro-kinetic energy drinking water, which is prepared by treating raw water of drinking water using the electromagnetic wave in non-contact mode, the ultraviolet absorption peak of the multi-polar micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably 28 nm to 30 nm, and most preferably 30 nm or 28 nm when compared with the raw water of drinking water.
Compared with the raw water of drinking water, the intensity of the fluorescence emission spectrum of the multi-polar micro-kinetic energy drinking water is greatly improved in the spectral range between 300nm and 400nm. The results from many repeated experiments on the same experimental sample show that the experiment has good reproducibility
The experiments show that the multi-polar micro-kinetic energy drinking water of the present invention has more extranuclear electrons in the non-radiative high energy level.
Preferably, the raw water of drinking water is ordinary tap water or various mineral water or purified water such as Evian Natural Mineral Water, Tibet Glacier Mineral Water, NongFu Spring, Wahaha and so on.
It is best to drink or use the multi-polar micro-kinetic energy drinking water of the present invention within 72 hours after preparation.
In another aspect, the present invention provides a preparation method of the
2015101916 20 Mar 2015 above-mentioned multi-polar micro-kinetic energy drinking water, which comprises treating raw water of drinking water using three different types of electromagnetic waves: low, medium and high frequency electromagnetic waves, in non-contact mode, wherein the frequency range of the low frequency electromagnetic wave is 30-100 kHz, the frequency range of the medium frequency electromagnetic wave is 550-720 kHz, the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
Preferably, the raw water of drinking water is ordinary tap water or various mineral water or purified water such as Evian Natural Mineral Water, Tibet Glacier Mineral Water, NongFu Spring, Wahaha and so on.
Preferably, the frequency range of the low frequency electromagnetic wave is 45-75 kHz.
Preferably, the frequency range of the medium frequency electromagnetic wave is 600-720 kHz.
Preferably, the frequency range of the high frequency electromagnetic wave is 300-425 MHz.
Preferably, the low frequency electromagnetic wave and the medium frequency electromagnetic wave are sine wave, square wave, sharp wave, sawtooth wave or trapezoidal wave.
Preferably, the waveform of the low frequency electromagnetic wave and the medium frequency electromagnetic wave may be the same or different, and are preferably the same.
Preferably, the low frequency electromagnetic wave and the medium frequency electromagnetic wave are propagated in the same direction.
Preferably, the high frequency electromagnetic wave is triangular wave, and its propagation direction is perpendicular to that of the low frequency electromagnetic wave and the medium frequency electromagnetic wave.
Preferably, when treating raw water of drinking water using low, medium and high frequency electromagnetic waves, which are three different types of
2015101916 20 Mar 2015 electromagnetic waves, in non-contact mode, firstly, the low-frequency electromagnetic wave and the high frequency electromagnetic wave are used simultaneously and then the medium electromagnetic wave and high frequency electromagnetic wave are used simultaneously
Preferably, the treating time with the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10 to 30 minutes, preferably 10 to 20 minutes; and the treating time with the medium frequency electromagnetic wave and the high frequency electromagnetic wave is 10 to 30 minutes, preferably 10 to 20 minutes.
Preferably, when compared with the raw water of drinking water, the ultraviolet absorption peak of the obtained multi-polar micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably 28 nm to 30 nm, and most preferably 30 nm or 28 nm.
Compared with raw water of drinking water, the intensity of the fluorescence emission spectrum of the multi-polar micro-kinetic energy drinking water of the present invention is greatly improved in the spectral range between 300nm and 400nm. The results from many repeated experiments on the same experimental sample show that the experiment has good reproducibility.
The experiments show that the multi-polar micro-kinetic energy drinking water of the present invention has more extranuclear electrons in the non-radiative high energy level.
In the present invention, the method and apparatus for generating the electromagnetic waves are conventional technical means in the art.
In yet another aspect, the present invention provides a use of the multi-polar micro-kinetic energy drinking water described above for the preparation of various mineral water, purified water, beverages, health care products or medicaments.
Preferably, the beverage is a functional beverage and can be used for inhibiting fatigue, losing weight, defaecating, lowering blood pressure, lowering
2015101916 20 Mar 2015 blood glucose, lowering blood uric acid and lowering blood urea.
Preferably, the health care products or medicaments can be used for inhibiting fatigue, losing weight, defaecating, lowering blood pressure, lowering blood glucose, lowering blood uric acid and lowering blood urea.
Compared with the prior art, the present invention treats water using the electromagnetic wave non-contact treatment water without adding any additives, the preparation method of the present invention is simple and has high production efficiency and low cost.
Compared with the existing water, the ultraviolet absorption peak of the multi-polar micro-kinetic energy drinking water of the present invention is shifted obviously to the short-wave direction relative to the untreated raw water of drinking water, and the intensity of the fluorescence emission spectrum of the multi-polar micro-kinetic energy drinking water is greatly improved in the spectral range between 300nm and 400nm, and more extranuclear electrons are in the non-radiative high energy level. Animal experiments have proven that the multi-polar micro-kinetic energy drinking water of the present invention has the following effects: inhibiting fatigue, losing weight, defaecating, lowering blood pressure, lowering blood glucose, lowering blood uric acid and lowering blood urea.
BRIEF DESCRIPTION OF THE DRAWINGS
Hereinafter, exemplary embodiments of the present invention will be described in detail with reference to the accompanying drawings, wherein:
Figure 1 shows the effect of the water from different treatment groups on DNA damage induced by peroxide;
Figure 2 shows the effect of the culture medium from different treatment groups on cells;
Figure 3 shows the absorption spectrum of raw water of ordinary drinking water and the multi-polar micro-kinetic energy water according to the present
2015101916 20 Mar 2015 invention obtained from the raw water of ordinary drinking water.
Figure 4 shows the absorption spectrum distribution of Evian Natural Mineral Water (raw water) and the multi-polar micro-kinetic energy water according to the present invention obtained from the Evian Natural Mineral Water.
Figure 5 shows the absorption spectrum distribution of Tibet Glacier Mineral Water (raw water) and the multi-polar micro-kinetic energy water according to the present invention obtained from the Tibet Glacier Mineral Water.
Figure 6 shows the fluorescence steady-state and transient result of ordinary drinking water (raw water) and the multi-polar micro-kinetic energy drinking water obtained from the ordinary drinking water;
Figure 7 shows the fluorescence steady-state and transient result of Evian Natural Mineral Water (raw water) and the multi-polar micro-kinetic energy drinking water obtained from the Evian Natural Mineral Water;
Figure 8 shows the fluorescence steady-state and transient result of Tibet Glacier Mineral Water (raw water) and the multi-polar micro-kinetic energy drinking water obtained from the Tibet Glacier Mineral Water;
Figure 9 shows the results of repeatability tests of the multi-polar micro-kinetic energy drinking water prepared from Tibet Glacier Mineral Water as the raw water in triplicate;
Figure 10 shows the effect of the multi-polar micro-kinetic energy drinking water of the present invention on uric acid (UA) of hyperuricemia rats;
Figure 11 shows the effect of the multi-polar micro-kinetic energy drinking water of the present invention on urea (UREA) of hyperuricemia rats;
Figure 12 shows the effect of the multi-polar micro-kinetic energy drinking water of the present invention on creatinine (CREA) of hyperuricemia rats.
DETAILED DESCRIPTION OF THE EMBODIMENTS
The invention will now be described in further detail with reference to
2015101916 20 Mar 2015 specific embodiments thereof, and the invention is given by way of illustration only and is not intended to limit the scope of the invention.
The experimental methods in the following examples are conventional methods unless otherwise specified. The raw materials, reagents and the like used in the following examples are commercially available unless otherwise specified.
Example 1 A preparation method of the multi-polar micro-kinetic energy drinking water of the present invention
Three different frequencies of electromagnetic waves: low, medium and high frequency electromagnetic waves, were applied at the outside of the pipe of raw water of ordinary drinking water in non-contact mode, wherein the frequency of the low frequency electromagnetic wave was 30 kHz, the frequency of the medium frequency electromagnetic wave was 550 kHz, and the frequency of the high frequency electromagnetic wave was 300 MHz.
Wherein, the low frequency electromagnetic wave and the medium frequency electromagnetic wave were both sine wave and they were propagated in the same direction. The high frequency electromagnetic wave was triangular wave, and its propagation direction was perpendicular to that of the low frequency electromagnetic wave and the medium frequency electromagnetic wave. When treatment, firstly, the low frequency electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 20 minutes, and then the medium electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 20 minutes. After the treatment was completed, the multi-polar micro-kinetic energy drinking water of the present invention was obtained.
Example 2 A preparation method of the multi-polar micro-kinetic energy drinking water of the present invention
Three different frequencies of electromagnetic waves: low, medium and high
2015101916 20 Mar 2015 frequency electromagnetic waves, were applied at the outside of the pipe of raw water of ordinary drinking water in non-contact mode, wherein the frequency of the low frequency electromagnetic wave was 100 kHz, the frequency of the medium frequency electromagnetic wave was 720 kHz, and the frequency of the high frequency electromagnetic wave was 725 MHz.
Wherein, the low frequency electromagnetic wave and the medium frequency electromagnetic wave were both sine wave and they were propagated in the same direction. The high frequency electromagnetic wave was triangular wave, and its propagation direction was perpendicular to that of the low frequency electromagnetic wave and the medium frequency electromagnetic wave. When treatment, firstly, the low frequency electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 10 minutes, and then the medium electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 10 minutes. After the treatment was completed, the multi-polar micro-kinetic energy drinking water of the present invention was obtained.
Example 3 A preparation method of the multi-polar micro-kinetic energy drinking water of the present invention
Three different frequencies of electromagnetic waves: low, medium and high frequency electromagnetic waves, were applied at the outside of the pipe of raw water of ordinary drinking water in non-contact mode, wherein the frequency of the low frequency electromagnetic wave was 45 kHz, the frequency of the medium frequency electromagnetic wave was 600 kHz, and the frequency of the high frequency electromagnetic wave was 300 MHz.
Wherein, the low frequency electromagnetic wave and the medium frequency electromagnetic wave were both sine wave and they were propagated in the same direction. The high frequency electromagnetic wave was triangular wave, and its propagation direction was perpendicular to that of the low
2015101916 20 Mar 2015 frequency electromagnetic wave and the medium frequency electromagnetic wave. When treatment, firstly, the low frequency electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 15 minutes, and then the medium electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 15 minutes. After the treatment was completed, the multi-polar micro-kinetic energy drinking water of the present invention was obtained.
Example 4 A preparation method of the multi-polar micro-kinetic energy drinking water of the present invention
Three different frequencies of electromagnetic waves: low, medium and high frequency electromagnetic waves, were applied at the outside of the pipe of raw water of ordinary drinking water in non-contact mode, wherein the frequency of the low frequency electromagnetic wave was 75 kHz, the frequency of the medium frequency electromagnetic wave was 720 kHz, and the frequency of the high frequency electromagnetic wave was 425 MHz.
Wherein, the low frequency electromagnetic wave and the medium frequency electromagnetic wave were both sine wave and they were propagated in the same direction. The high frequency electromagnetic wave was triangular wave, and its propagation direction was perpendicular to that of the low frequency electromagnetic wave and the medium frequency electromagnetic wave. When treatment, firstly, the low frequency electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 20 minutes, and then the medium electromagnetic wave and the high frequency electromagnetic wave were used simultaneously for 20 minutes. After the treatment was completed, the multi-polar micro-kinetic energy drinking water of the present invention was obtained.
Example 5 Physical and chemical properties of the multi-polar
2015101916 20 Mar 2015 micro-kinetic energy drinking water of the present invention
In accordance with the Standard examination methods for drinking water (GB/T 5750-2006), the multi-polar micro-kinetic drinking water according to examples 1 to 4 of the present invention was tested and the testing results were basically the same. The results are shown in Table 1.
Table 1 physical and chemical properties of the multi-polar micro-kinetic
| energy drinking water of the | ?resent invention | ||||
| Nos. | Testing items | Units | National standards | Testing results | Conclusions |
| 1 | Chroma | Degree | <15 | <5 | Qualified |
| 2 | Turbidity | NTU | <1 | 0.39 | Qualified |
| 3 | Smell and taste | Description | none | none | Qualified |
| 4 | Visible material to the naked eye | Description | none | none | Qualified |
| 5 | pH | 6.5-8.5 | 8.00 | Qualified | |
| 6 | Total hardness | mg/L | <450 | 164 | Qualified |
| 7 | Aluminum (Al) | mg/L | <0.2 | <0.01 | Qualified |
| 8 | Iron (Fe) | mg/L | <0.3 | 0.089 | Qualified |
| 9 | Manganese (Mn) | mg/L | <0.1 | 0.0082 | Qualified |
| 10 | Copper (Cu) | mg/L | <1 | <0.01 | Qualified |
| 11 | Zinc (Zn) | mg/L | <1 | 0.108 | Qualified |
| 12 | Sulfates | mg/L | <250 | 87.4 | Qualified |
| 13 | Chlorides | mg/L | <250 | 47.3 | Qualified |
| 14 | Total dissolved solids | mg/L | <1000 | 396 | Qualified |
| 15 | Oxygen consumption | mg/L | <3 | 0.68 | Qualified |
| 16 | Volatile phenols | mg/L | <0.002 | <0.002 | Qualified |
| 17 | Anionic synthetic detergents | mg/L | <0.3 | <0.10 | Qualified |
| 18 | Cyanides | mg/L | <0.05 | <0.002 | Qualified |
2015101916 20 Mar 2015
| 19 | Fluorides | mg/L | <1.0 | 0.50 | Qualified |
| 20 | Arsenic (As) | mg/L | <0.01 | <0.0005 | Qualified |
| 21 | Selenium (Se) | mg/L | <0.01 | 0.0014 | Qualified |
| 22 | Mercury (Hg) | mg/L | <0.001 | <0.00005 | Qualified |
| 23 | Cadmium (Cd) | mg/L | <0.005 | <0.0001 | Qualified |
| 24 | Chromium (hexavalent) (Cr(VI)) | mg/L | <0.05 | <0.004 | Qualified |
| 25 | Nitrate nitrogen | mg/L | <10 | 0.08 | Qualified |
| 26 | Lead(Pb) | mg/L | <0.01 | <0.0005 | Qualified |
| 27 | Trichloromethane | mg/L | <60 | <0.5 | Qualified |
| 28 | Carbon Tetrachloride | mg/L | <2 | <0.1 | Qualified |
According to the above table, it can be seen that the micro-kinetic energy drinking water of the present invention meet the requirements of Standard examination methods for drinking water (GB/T 5750-2006).
Example 6 Toxicology experiment of the multi-polar micro-kinetic energy drinking water of the present invention
I.Acute oral toxicity test
1. Materials and Methods
1.1 Test substance: the micro-kinetic drinking water prepared in Example 3
1.2 Animals: Kunming mice, which were obtained from Institute of Laboratory Animal Resources, National Institute for Food and Drug Control (certificate number: SCXK (Beijing) 2009-0017), SPF level, weight 18.0-22.0 g; before testing, the animals were fasted overnight and allowed to intake water freely.
1.3 Groups according to dose: the dose of the test substance was 20000 mg/kg body weight, 10 female animals and 10 male animals in each group. They were caged by sex to feed. They were infected by a single oral gavage at a dose
2015101916 20 Mar 2015 of 0.1ml/10g body weight.
1.4 Observations: after infection, the animal’s general situation, infected symptoms and death were observed. The observation period was two weeks. At the end of the trial, all animals were dissected to record the general pathological changes. Acute toxicity was graded according to the acute toxicity rating criteria.
2. Test results
Test results of acute oral toxicity test in mice
| Sex | Dose (mg/kg) | Number of Animals | Number of Dead Animals | Mortality Rate (%) |
| Female | 20000 | 10 | 0 | 0 |
| Male | 20000 | 10 | 0 | 0 |
3. Conclusion
After infecntion, the animals do not show any infected symptoms and have no death and no significant pathological changes, and on the contrary, the weight of the animals showed an increasing trend. Therefore, the LD50 of the test substance on female and male mice by oral was greater than 10000 mg/kg body weight, so the test substance belongs to the actual non-toxic level.
II. Effect on DNA damage induced by peroxide
1. Experimental materials and equipment
1.1 5mM Vitamin C solution: it was prepared by dissolving it in three distilled water and it needs to be prepared before use. Vitamin C powder was obtained from Sigma-Aldrich;
1.2 5mM CuCl2 solution: it was prepared by dissolving it in three distilled water and it needs to be prepared before use. CuCl2 powder was obtained from Sinopharm Chemical Reagent Co. Ltd.;
1.3 Plasmid DNA: it was obtained by amplifmg and extracting the pET28a plasmid from DH5a Escherichia coli (the plasmid extraction kit was purchased
2015101916 20 Mar 2015 from Promega);
1.4 5mMEDTA solution, pH8.0: EDTA was obtained from Sinopharm Chemical Reagent Co. Ltd.;
1.5 lOxPBS buffer: 80g NaCl, 2g KC1, 14.4g Na2HPO4 and 2.4g KH2PO4 were dissolved in distilled water, the pH was adjusted to 7.4 and the volume was adjusted to IL. The required reagents were purchased from Sinopharm Chemical Reagent Co. Ltd.;
1.6 Agarose: obtained from Bio west;
1.7 Goldview nucleic acid dyes were obtained from Biotium;
1.8 Gel Imager: obtained from Sage Creation Science And Technology Co., Ltd;
1.9 Electrophoresis apparatus: LiuYi instrument factory;
1.10 Test substance: the micro-kinetic drinking water of Examples 3 and 4
2. Experimental methods
2.1 Group of Experiments
Group I: untreated group, the untreated sterile water was applied to the reaction system;
Group II: the micro-kinetic drinking water of example 3;
Group III: the micro-kinetic drinking water of example 4.
The reaction substances were added according to the following order, the final volume of the reaction system was 10 pL:
lOxPBS buffer 1 pL;
Plasmid DNA (2 pg) 2 pL;
mM Vitamin C 1 pL;
mM CuCl2 1 pL;
supplemented with the untreated water or treated water to 10 pL.
2.2 The reaction was terminated by adding lpL of 5 mM EDTA and 2.2 pL of loading buffer at 0.5 hour and 1 hour respectively;
2.3 0.8% agarose gel (containing Goldview dye), electrophoresis 1 h;
2015101916 20 Mar 2015
2.4 Gel imaging.
3. Experimental results
Vitamin C and divalent copper ions occur redox reaction to form peroxide, which will cause plasmid DNA damage and fracture, this embodies in the weakening of the electrophoretic bands. The experimental results are shown in figure 1, the experiment is divided into three groups, the grouping method was as described above, each group is reacted at two time points: 0.5 hours and 1 hour, the brightness of DNA represents DNA content. According to the experimental results, the degradation rate of plasmid DNA by redox reaction was basically the same in group II and III compared with untreated group I, and the micro-kinetic drinking water does not prevent or accelerate DNA damage and fracture.
4. Analysis on the results
Vitamin C and divalent copper ions occur redox reaction to form peroxide, which will cause plasmid DNA damage and fracture, this embodies in the weakening of the electrophoretic bands. The experimental results showed the degradation rate of plasmid DNA by redox reaction was basically the same in group II and III compared with untreated group I, and the micro-kinetic drinking water did not prevent or accelerate DNA damage and fracture. It is shown that the multi-polar micro-kinetic energy water of the present invention has no effect on DNA damage induced by peroxide.
III. Effect on cell membrane integrity of HepG2 cultured in vitro
1. Experimental materials and equipment
1.1 Human hepatocellular carcinoma cells: HepG2;
1.2 Cell culture medium: DMEM culture medium+10%FBS;
1.3 FAM-aptamer: purchased from Sangon Biotech (Shanghai) Co., Ltd.;
1.4 Fluorescent EZ micromirrors: OLYMPUS;
1.5 96-well cell culture plate;
2. Experimental methods
2015101916 20 Mar 2015
2.1 A proper amount of pancreatic enzyme was added to digest the cells in logarithmic growth phase. The cell concentration was adjusted, and the cell was seeded in 96-well plates at 4000 cells per well and cultured overnight;
2.2 5 ml of DMEM culture medium containing 10% FBS (culture medium A) was treated in the same manner as in Example 3 for 40 minutes (treatment group);
2.3 5 ml of DMEM culture medium containing 10% FBS (culture medium B) was treated for 40 minutes at the position which was more than 2m away from the wave source of example 3 (untreated group);
2.4 4 pg of FAM-labeled C6-8 aptamer was added to 100 pL of culture medium A and culture medium B respectively and mixed uniformly;
2.5 The cell fluid adherent cultured in the 96-well plate were replaced with culture medium A and culture medium B containing FAM-C6-8 aptamer, incubated at 37 °C for 30 min, and then washed once with PBS and observed under the fluorescence microscope.
3. Experimental results
The results were shown in figure 2, there was no difference in cell morphology between the treated and untreated groups, the cells in the two groups showed autofluorescence and had intact cell membranes, and no apparent FAM-labeled C6-8 aptamer green fluorescence was observed in the cells.
4. Results and analysis
If the cells are perforated, the FAM-labeled C6-8 aptamer can enter the cell and bind with the protein in the cell and emit green fluorescence upon excitation at 530nm wavelengths. The results show that there is no difference between the cells in the treatment group and that in the untreated group, the cells in the two groups showed autofluorescence, which indicates that the cell membrane is intact. The multi-polar micro-kinetic energy drinking water of the present invention has no effect on cell morphology and cell membrane integrity.
2015101916 20 Mar 2015
Example 7 Wide spectral absorptance and UV absorptance of the multi-polar micro-kinetic energy drinking water of the present invention
1. Experimental materials and equipment
1.1 The micro-kinetic energy drinking water prepared in Example 3
1.2 The raw water of ordinary drinking water used in Example 3;
1.3 The drinking water prepared from Evian Natural Mineral Water, which was treated in the same manner as in Example 3;
1.4 Evian Natural Mineral Water: purchased from Carrefour supermarket;
1.5 The drinking water prepared from Tibet Glacier Mineral Water, which was treated in the same manner as in Example 3;
1.6 Tibet Glacier Mineral Water: purchased from Carrefour supermarket;
1.7 Ultra-pure water: prepared by the German Sartorius ultra-pure water preparation system;
1.8 Fiber optic spectrometer Ava-Spec3648, the measuring range is 200-1100 nm, the uncertainty/accuracy is 0.5nm;
1.9 Oscilloscope MS04104, equipment factory number: C001163, measurement accuracy: 1 GHz;
1.10 Photodetector Thorlab DET10A/M, equipment factory number: JGZX-ZXZC-012, measurement accuracy: 200 nm-1100 nm;
1.11 Test conditions: the temperature is 21 °C, the humidity is 38%.
2. Experimental methods
2.1 Wide spectrum absorptance (1) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes were stable.
(2) Placing the raw water of ordinary drinking water used in Example 3 and the micro-kinetic energy drinking water prepared in Example 3 in an experimental vessel, and scanning the spectral absorption of the standard light source of sample at 200 nm to 800 nm, and the experimental results were recorded.
2015101916 20 Mar 2015 (3) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability (4) Placing the Evian Natural Mineral Water and the drinking water prepared from the Evian Natural Mineral Water by using the same manner as Example 3 in the experimental vessels, repeating the step (2), and then recording the changes in UV intensity during the experimental period.
(5) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability.
(6) Placing the Tibet Glacier Natural Mineral Water and the drinking water prepared from the Tibet Glacier Natural Mineral Water by using the same manner as Example 3 in the experimental vessels, repeating the step (2), and then recording the changes in UV intensity during the experimental period.
(7) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability.
2.2 UV absorptance (1) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes were stable.
(2) Placing the micro-kinetic energy drinking water prepared in example 3 in an ultraviolet intensity detection system, irradiatting the water to be measured using the ultraviolet light source, observaing the ultraviolet intensity value of the water to be measured through the oscilloscope MS04104 and the photodetector Thorlab DET10A/M for 72 hours continuously, and recording the changes in UV intensity during the experimental period.
(3) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability.
2015101916 20 Mar 2015 (4) Placing the Evian Natural Mineral Water and the drinking water prepared from the Evian Natural Mineral Water by using the same manner as Example 3 in the ultraviolet intensity detection system, repeating the step (2), and then recording the changes in UV intensity during the experimental period.
(5) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability.
(6) Placing the Tibet Glacier Natural Mineral Water and the drinking water prepared from the Tibet Glacier Natural Mineral Water by using the same manner as Example 3 in the the ultraviolet intensity detection system, repeating the step (2), and then recording the changes in UV intensity during the experimental period.
(7) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability.
3. Experimental results
3.1 Wide spectrum absorptance test
The results are shown in Figure 3-5.
3.2 UV absorptance test
The multi-polar micro-kinetic energy drinking water prepared from ordinary water:
UV absorptance 0.9135 -> 0.9037
The multi-polar micro-kinetic energy drinking water prepared from Evian Natural Mineral Water:
UV absorptance 0.9244 -> 0.9188
The multi-polar micro-kinetic energy drinking water prepared from Tibet Glacier Mineral Water:
UV absorptance 0.9157 -> 0.9079
4. Experimental conclusions and analysis
2015101916 20 Mar 2015
Through the above experiments, the following conclusions are obtained:
(1) Compared with the raw water, the absorption peak of the absorption spectrum of the multi-polar micro-kinetic energy drinking water of the present invention is shifted to the short wave by about 30 nm;
(2) The absorbance of the multi-polar micro-energy drinking water of the present invention decreased by 1% after testing for 72 hours.
Example 8 The stimulated fluorescence spectrum change of the multi-polar micro-kinetic energy drinking water of the present invention
1. Experimental materials and equipment
1.1 The micro-kinetic energy drinking water prepared in Example 3
1.2 The raw water of ordinary drinking water used in Example 3;
1.3 The drinking water prepared from Evian Natural Mineral Water, which was treated in the same manner as in Example 3;
1.4 Evian Natural Mineral Water: purchased from Carrefour supermarket;
1.5 The drinking water prepared from Tibet Glacier Mineral Water, which was treated in the same manner as in Example 3;
1.6 Tibet Glacier Mineral Water: purchased from Carrefour supermarket;
1.7 Ultra-pure water: prepared by the German Sartorius ultra-pure water preparation system;
1.8 Fiber optic spectrometer Ava-Spec3648, equipment factory number is 1101230U1, the measuring range is 200-1100 nm, and the uncertainty/accuracy is 0.5nm;
1.9 Test conditions: the temperature is 22 °C, and the humidity is 37 %.
2. Experimental methods (1) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes were stable.
(2) Placing the micro-kinetic energy drinking water prepared in Example 3 in a fiber-optic spectrometer sample pool, enforcing an excitation pulse on the
2015101916 20 Mar 2015 sample with a 266 nm pulsed ultraviolet laser, and measuring the emission spectrum of the samples using the scanning function on the fiber-optic spectrometer and recording the fluorescence emission spectra of the samples.
(3) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability.
(4) Placing the raw water of ordinary drinking water used in Example 3 in the fiber-optic spectrometer sample pool, repeating the step (2), and then recording the changes in UV intensity during the experimental period.
(5) Rinsing the experimental vessels by using about 200 ml ultra-pure water till the physical and chemical indexes restored the original value and reached stability.
(6) Placing the drinking water prepared from Evian Natural Mineral Water by using the same manner as in Example 3 in the fiber-optic spectrometer sample pool, repeating the steps (2) and (3), and then recording the experimental results.
(7) Placing the Evian Natural Mineral Water in the fiber-optic spectrometer sample pool, repeating the steps (2) and (3), and then recording the experimental results.
(8) Placing the drinking water prepared from Tibet Glacier Natural Mineral Water by using the same manner as in Example 3 in the fiber-optic spectrometer sample pool, repeating the steps (2) and (3), and then recording the experimental results.
(9) Placing the Tibet Glacier Natural Mineral Water in the fiber-optic spectrometer sample pool, repeating the steps (2) and (3), and then recording the experimental results.
(10) Placing the drinking water prepared from Tibet Glacier Natural Mineral Water in triplicate by using the same manner as in Example 3 in the fiber-optic spectrometer sample pool, repeating the steps (2) and (3), and then recording the experimental results.
2015101916 20 Mar 2015
3. Experimental results
3.1 Fluorescence steady-state transient test
The fluorescence steady-state transient test results of ordinary drinking water and the multi-polar micro-kinetic energy drinking water prepared from the same are shown in Figure 6. The fluorescence steady-state transient test results of Evian Natural Mineral Water and the multi-polar micro-kinetic energy drinking water prepared from the same are shown in Figure 7. The fluorescence steady-state transient test results of Tibet Glacier Mineral Water and the multi-polar micro-kinetic energy drinking water prepared from the same are shown in Figure 8. The repeatability test results of the drinking water prepared from Tibet Glacier Natural Mineral Water in triplicate are shown in Figure 9.
4. Experimental conclusions and analysis
From the above experimental results, it can be seen that compared with raw water of drinking water, the intensity of the fluorescence emission spectrum of the multi-polar micro-kinetic energy drinking water is greatly improved in the spectral range between 300nm and 400nm. The results from many repeated experiments on the same experimental sample show that the experiment has good reproducibility.
The experiments show that the multi-polar micro-kinetic energy drinking water of the present invention has more extranuclear electrons in the non-radiative high energy level.
Example 9 Effect of the multi-polar micro-kinetic energy drinking water of the present invention on hyperuricemia SD rats induced by oteracil potassium
1. Materials and Method
1.1 Samples:
The multi-polar micro-kinetic energy drinking water (the kinetic energy water) prepared in Example 3, which is a colorless, tasteless and transparent
2015101916 20 Mar 2015 water-like liquid, stored at room temperature and should be used within 72 hours after being prepared.
Positive-drug benzbromarone: Yichang Changjiang Pharmaceutical Co. Ltd. The batch number: 0061402004.
1.2 Experimental animals: SD rats were purchased from Beijing Huafu Kang Biotechnology Co. Ltd.. The production license number: SCXK (Beijing) 2007-0001. There are 70 in all, all of them are males, the ordering weight is 160-180 g. 60 rats were selected for formal testing after screening.
Feeding conditions: the laboratory animal facilities continue to maintain the barrier environmental standards. The main environmental indicators are as follows: the room temperature is 20 ~ 26 °C, the daily temperature difference is <4 °C. The relative humidity is 40 to 70%. The minimum ventilation time is 15 times per hour. Lighting: Dark = 12 h: 12 h. Animals were housed in PC mice rearing cages and the specifications of cages were 545x395x200 mm , 5 in each cage, and their space was in accordance with the provisions of GB 14925-2010 of the People's Republic of China on the minimum required space for experimental animals. All animals are fed and managed by trained and qualified personnel. The padding and cage are replaced once a week. And the special feed for mice is added every day for animal consumption and the animal diet activities are kept free during the whole feeding process.
Animal welfare: the animal and related treatment used in this test should meet the requirements of animal welfare. Experiments were carried out after being reviewed and approved by the Laboratory's Institutional Animal Care and Use Committee (IACUC), Beijing Jianhao Pharmaceutical Technology Development Co. Ltd.. The specific considerations are as follows:
(1) During the course of the experiment, the animal's panic and pain should be minimized.
(2) Effective anesthesia must be performed in the operation and dissection of experimental animals. Analgesic, targeted care and diet conditioning should be
2015101916 20 Mar 2015 performed based on the actual situation during postoperative recovery period.
(3) Animal disturbances, panic, pain and injure should be avoided in the experiments of fixation of animal. Fixation machine should have a reasonable structure, appropriate specification, should be durable, environmentally friendly and easy to operate. Without prejudice to the experiment, the mandatory restrictions on the animal's body should be reduced to a minimum.
(4) Sample collection: the operation of the sample collection of experimental animals should be carried out in a safe and humane manner (to minimize stress and discomfort caused to animals).
(5) Dying animals, diseased animals and animals with severe toxicity should be promptly reported to the veterinarian and the person in charge to timely treat or euthanize. Without affecting the determination of the experiment results, “humane endpoint should be chosen to avoid prolonging the time when the animals suffer from pain. It should be euthanized in accordance with the humanitarian principles when the animals are sacrificed. At the scene of the execution, there should be no other animals present. The body can be properly disposed of after confirmation of the death of the animal.
(6) Occupational safety: personal protective measures such as gloves, gowns, masks, goggles and earplugs etc. should be taken during the experiment.
1.4 Main instruments and reagents:
AU480 automatic biochemical analyzer, determination kit for blood uric acid, urea and creatinine (Zhongsheng reagent company).
1.5 Test methods:
1.5.1 Compliance criteria: Yang Guimei et al., establishment of hyperuricemia model in rats, Laboratory Animal Science, 2011; and Liu Shufen et al., Advances in research of animal models of hyperuricemia, Basic and Clinical Medicine, 2011.
1.5.2 Detection Indicators: animal general condition, body weight, growth status, blood uric acid, urea and creatinine.
2015101916 20 Mar 2015
1.5.3 Animal grouping and dose design: animals were collected and adapted to quarantine for 2 weeks. At the end of the quarantine, 70 animals were selected and randomly divided into 6 groups according to the measurement results of blood uric acid, and each group had 10 animals. Ten animals were selected as the blank control group and 50 animals were administrated 0.70 g/kg of oteracil potassium (0.10 g/ml) by gavage per day to prepare hyperuricemia models. The animals in the blank control group drank normal drinking water daily; In the low dose kinetic energy water group, the animals drank the kinetic energy water daily (the kinetic energy water was kept in a drinking bottle, and supplemented once a day); in addition to drinking the kinetic energy water daily, in the high dose kinetic energy water group, the animals were given the kinetic energy water by gavage once a day, the dose was 1.5 ml/100 g; in the positive-drug benzbromarone group, the animals were given by gavage once a day, the administration dose was 10 mg/kg and the administration volume was 1.5 mL/100 g (the daily dosage of the benzbromarone tablets per person is 50 mg, if the weight of a person is 60kg, the dosage on the person is equivalent to 0.833 mg/kg, if the dosage used in mice is 10 times of that used in the person, the dosage on the mice is equivalent to 100 mg/kg, the administration volume is 1.5 mL/kg and the concentration of drugs is 0.67 mg/ml); In addition, a group of benzbromarone and high-dose kinetic energy water was established and the dose of benzbromarone tablet prepared by kinetic energy water was 10 mg/kg and the administration volume was 1.5 mL/100 g.
Table 2 Effect of the kinetic energy water of the present invention on the levels of uric acid, urea and creatinine in hyperuricemia SD rats induced by oteracil potassium
| Numb | |||||
| Group | Dosage | Administration volume | er of | Cage | Serial number |
| anima | number | ||||
| Is |
2015101916 20 Mar 2015
| Blank control group | 10 | 1,2 | 1-10 | ||
| Model group | - | - | 10 | 3,4 | 11-20 |
| Low-dose kinetic energy water | Daily drinking | Daily drinking | 10 | 5,6 | 21-30 |
| group | |||||
| High-dose kinetic energy water | Daily drinking +1.5 mL/100 g | Daily drinking +1.5 mL/100 g | 10 | 7,8 | 31-40 |
| group | |||||
| Benzbromaro ne group | 10 mg/kg | 1.5 mL/100 g | 10 | 9, 10 | 41-50 |
| high-dose kinetic energy water and benzbromaro ne group | Daily drinking | Daily drinking | |||
| +1.5 mL/100 g (containing 10 | +1.5 mL/100 g (containing | 10 | 11, 12 | 51-60 | |
| mg/kg of drugs) | 0.67 mg/ml of drugs) |
1.5.4 Administration time: continuous administration for 6 weeks.
1.5.5 The measuring time:
Blood uric acid, blood creatinine and urea were measured respectively at the time before administration, the fifteenth day of administration (DI5), the thirty-fourth day of administration (D34) and the forty-third day of administration (D43). The blood samples in the first three times were collected from inner canthus, and the blood sample in the last time was collected from abdominal aorta.
1.6 Statistics and analysis of the test data
A SPSS statistical software was used to process the results of body weight and compare and analyze the administration and control group. Statistical
2015101916 20 Mar 2015 analysis was done according to the following methods: the normal test was performed using Kolmogorov-Smirnov method and the test for homogeneity of variance was performed using Levene median method, if P>0.05, then one-way ANOVA method will be performed, and if the normal test and the test for homogeneity of variance failed (P<0.05), then a nonparametric Mann-Whitney test will be required.
1.7 Determination of results
Determination of results: if the uric acid level of the animals in the model group was higher than that in the blank control group or the uric acid level of the animals in the bromambalone group was significantly lower than that in the model control group and the difference was significant, then this indicated that the hyperuricemia model was established successfully. If the uric acid level of the animals in the kinetic energy water group was significantly lower than that in the model control group and the difference was significant, then this indicated that the kinetic energy water had the function of lowering uric acid. If the uric acid value in the benzbromarone and kinetic energy water group was lower than that in the benzbromarone group, then this indicated that the kinetic energy water had the effect of enhancing the function of benzbromarone tablet in lowering uric acid, and the determination of urea and creatinine level was carried out at the same time. The criteria for determining the efficacy of the administration groups was the same as above.
2. Results:
Table 3 Effect of the kinetic energy water of the present invention on blood uric acid in hyperuricemia SD rats induced by oteracil potassium (pmol/L, x±5, n=10)
| Blank | Low-dose | High-dose | Benzbromarone | |||
| Measurement | control | Model control | kinetic | kinetic | Benzbromarone | and kinetic |
| time | group | group | energy | energy water | group | energy water |
| water group | group | group |
2015101916 20 Mar 2015
| D3 | 90.0±l 1.4 | 86.3±17.4 | 83.6±9.4 | 89.7±16.7 | 86.7±18.3 | 81.2M9.8 |
| D15 | 82.0±17.6 | 106.0±14.7* | 115.8±9.1 | 120.6±15.4## | 115.0±13.9 | 127.3±8.4## |
| D34 | 98.4±19.5 | 95.3±13.1 | 82.2±12.5 | 92.4±7.6## | 79.2±22.9 | 73.4±9.3## |
| D43 | 73.1±11.0 | 216.2±20.1 | 198.6±19.0 | 198.7M7.6 | 139.9±30.5## | 137.2±22.9## |
Remark: **, compared with the blank control group, p<0.01; # ##, compared with the model control group, p<0.05 > p<0.01.
It can be seen from Table 3 and Figure 10 that the uric acid (UA) before administration of the rats in each group was consistent and there was no statistical difference.
Oteracil potassium was continuously administered for 15 days, compared with the blank control group, the uric acid value of animals in the model control group was increased to some extent (106.1±I4.7**, vs 82.0±17.6, pmol/L, **p<0.01). Oteracil potassium was continuously administered for 34 days, the results of the model group and the control group were consistent, and there was no statistically significant difference between them (95.3±13.1, vs 98.4±19.5, pmol/L, p>0.05). Oteracil potassium was continuously administered for 43 days, compared with the blank control group, the uric acid value of animals in the model control group was significant increased, the increase rate was 195.8% and the difference was statistically significant (216.2±20.1**, vs 73.1±1I.O, pmol/L, **p<0.01). This showed that the animal body can self-regulate in the process of modeling by administrating oteracil potassium. The establishment of the hyperuricemia model was successful when oteracil potassium was continuously administered for 43 days.
Compared with the model control group, after continuously drinking the kinetic energy water for 43 days, the blood uric acid value of rats in the low-dose and high-dose group reduced to a certain extent, the reduction rate was 8%, but the difference was not significant (198.6±19.0, 198.7±17.6, vs 216.2±20.1, pmol/L, p>0.05). The blood uric acid significantly decreased in the 10 mg/kg benzbromarone group and the 10 mg/kg benzbromarone and the kinetic energy
2015101916 20 Mar 2015 water group and the difference was significant (139.9±30.5##, 137.2±22.9##, vs 216.2±20.1, μηιοΙ/L, p>0.05).
Table 4 Effect of the kinetic energy water of the present invention on blood urea in hyperuricemia SD rats induced by oteracil potassium (mmol/L, x ± 5, n=10)
| Blank | Low-dose kinetic energy water group | High-dose kinetic energy water group | Benzbromarone group | Benzbromarone and kinetic energy water group | ||
| Measurement time | control group | Model control group | ||||
| D3 | 4.73±1.05 | 4.61M.34 | 5.60±1.53 | 5.32±0.72 | 4.89±0.97 | 5.34±0.44 |
| D15 | 4.49±0.62 | 4.16±0.75 | 3.83±0.37 | 3.28±0.37## | 2.26±0.70## | 2.06±0.35## |
| D34 | 5.89±0.78 | 5.47±0.41 | 5.85±0.50 | 5.70±0.26 | 5.76±0.64 | 6.00±0.60## |
| D43 | 4.71±0.74 | 7.91±0.93** | 6.26±1.33## | 5.09±1.20## | 2.90±0.66## | 2.53±0.29## |
Remark: **, compared with the blank control group, p<0.01; # ##, compared with the model control group, p<0.05, p<0.01.
It can be seen from Table 4 and Figure 11 that oteracil potassium was continuously administered for 15 days and for 34 days, compared with the blank control group, the urea value of animals in the model control group was consistent with the absolute value of animals in the blank control group and there was no statistical difference (D15: 4.16±0.75, vs 4.49±0.62; D34: 5.47±0.41, vs 5.89±0.78, mmol/L, p>0.05). Oteracil potassium was continuously administered for 43 days, compared with the blank control group, the urea value of animals in the model control group significant increased, the increase rate was 67.9%, and the difference was statistically significant (7.91±0.93**, vs 4.7H0.74, mmol/L, ** p<Q.Q 1). This showed that after continuously administrating oteracil potassium for 43 days (oteracil potassium was administrated for modeling), the establishment of hyperuricemia model was successful while the blood urea value of rats significantly increased.
Compared with the model control group, after continuously drinking the kinetic energy water for 43 days, the blood urea value of rats in the low-dose and high-dose group significantly decreased, the reduction rate was 20.8% and 35.7%
2015101916 20 Mar 2015 respectively, but the difference was significant (6.26±1.33##, 5.09±1.20##, vs 7.9H0.93, mmol/L, ##p<0.01). The blood urea value also significantly decreased in the 10 mg/kg benzbromarone group and the 10 mg/kg benzbromarone and the kinetic energy water group and the difference was significant (2.90±0.66##, 2.53±0.29##, vs 7.9H0.93, mmol/L, ## p<0.01).
Table 5 Effect of the kinetic energy water of the present invention on the blood creatinine in hyperuricemia SD rats induced by oteracil potassium (pmol/L, x±5,n=10)
| Blank | Low-dose kinetic energy water group | High-dose kinetic energy water group | Benzbromarone group | benzbromarone and kinetic energy water group | ||
| Measurement time | control group | Model control group | ||||
| D3 | 32.7+5.1 | 30.2+4.4 | 32.3+4.4 | 28.8+4.8 | 32.6+5.3 | 30.7+6.0 |
| D15 | 35.4+5.3 | 35.3+5.9 | 35.1+6.0 | 32.8+4.5 | 29.7+4.7° | 26.0+2.1 |
| D34 | 40.1+5.7 | 38.1+5.8 | 40.8+4.5 | 39.5+3.9 | 42.6+6.9 | 38.7+3.6 |
| D43 | 34.9+1.5 | 38.4+4.3* | 41.0+1.9 | 40.1+2.1 | 40.1+2.2 | 37.8+2.3 |
Remark: **, compared with the blank control group, p<0.05; # ##, compared with the model control group, p<0.05 > p<0.01.
It can be seen from Table 5 and Figure 12 that oteracil potassium was continuously administered for 15 days and for 34 days, compared with the blank control group, the blood creatinine value of animals in the model control group was consistent with the absolute value of animals in the blank control group and there was no statistical difference (D15: 35.3±5.9, vs 35.4±5.3; D34: 38.1±5.8, vs 40.1±5.7, pmol/L, p>0.05). Oteracil potassium was continuously administered for 43 days, compared with the blank control group, the blood creatinine value of animals in the model control group slightly increased, the increase rate was 10.2 %, and the difference was statistically significant (38.4±4.3**, vs 34.9=1=1.5, mmol/L, ** p<0.05). This showed that continuously administrating oteracil potassium for 43 days (oteracil potassium was administrated for modeling), the establishment of hyperuricemia model was successful while the blood creatinine
2015101916 20 Mar 2015 value of rats slightly increased.
Compared with the model control group, after continuously drinking the kinetic energy water for 43 days, there was no significant change in the blood creatinine value between the low-dose and high-dose group and the difference was not significant (41.0±1.9, 40.1±2.1, vs 38.4±4.3, pmol/L, p>0.05). There was no significant change in the blood creatinine value between the 10 mg/kg benzbromarone group and the 10 mg/kg benzbromarone and the kinetic energy water group and the difference was significant (41.0±2.2, 37.8±2.3, vs 38.4±4.3, pmol/L,p>0.05).
3. Conclusion
Based on the above results, under the experimental conditions, after continuously drinking the multi-polar micro-kinetic energy drinking water of the present invention for 43 days, the blood uric acid value of the hyperuricemia SD rats induced by oteracil potassium can be reduced to a certain extent, the blood urea value of the hyperuricemia SD rats induced by oteracil potassium can be significantly reduced, and the creatinine value was not affected.
The results showed that the kinetic energy water can decrease the raised blood uric acid value of the hyperuricemia SD rats induced by oteracil potassium to a certain extent and can obviously decrease the raised blood urea of the hyperuricemia SD rats induced by oteracil potassium.
Claims (10)
- Claims1. Use of a multi-polar micro-kinetic energy drinking water in the preparation of beverages, health care products or medicaments for reducing blood urea.
- 2. The use according to claim 1, wherein the multi-polar micro-kinetic energy drinking water was prepared by treating raw water of drinking water using three different types of electromagnetic waves: low, medium and high frequency electromagnetic waves, in non-contact mode, wherein the frequency range of the low frequency electromagnetic wave is 30-100 kHz, the frequency range of the medium frequency electromagnetic wave is 550-720 kHz, the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
- 3. The use according to claim 1 or 2, wherein the raw water of drinking water is ordinary tap water or various mineral water or purified water such as Evian Natural Mineral Water, Tibet Glacier Mineral Water, NongFu Spring, Wahaha and so on.
- 4. The use according to any one of the claims 1 to 3, wherein wherein the frequency range of the low frequency electromagnetic wave is 45-75 kHz;preferably, the frequency range of the medium frequency electromagnetic wave is 600-720 kHz;preferably, the frequency range of the high frequency electromagnetic wave is 300-425 MHz.
- 5. The use according to any one of the claims 1 to 4, wherein the low frequency electromagnetic wave and the medium frequency electromagnetic wave are sine wave, square wave, sharp wave, sawtooth wave or trapezoidal2015101916 20 Mar 2015 wave;preferably, the waveform of the low frequency electromagnetic wave and the medium frequency electromagnetic wave may be the same or different;more preferably, the waveform of the low frequency electromagnetic wave and the medium frequency electromagnetic wave is the same.
- 6. The use according to any one of the claims 1 to 5, wherein the low frequency electromagnetic wave and the medium frequency electromagnetic wave are propagated in the same direction.
- 7. The use according to any one of the claims 1 to 6, wherein tthe high frequency electromagnetic wave is triangular wave, and its propagation direction is perpendicular to that of the low frequency electromagnetic wave and the medium frequency electromagnetic wave.
- 8. The use according to any one of the claims 1 to 7, wherein when treating raw water of drinking water using low, medium and high frequency electromagnetic waves, which are three different types of electromagnetic waves, in non-contact mode, firstly, the low-frequency electromagnetic wave and the high frequency electromagnetic wave are used simultaneously and then the medium electromagnetic wave and high frequency electromagnetic wave are used simultaneously;preferably, the treating time with the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10 to 30 minutes, preferably 10 to 20 minutes;preferably, the treating time with the medium frequency electromagnetic wave and the high frequency electromagnetic wave is 10 to 30 minutes, preferably 10 to 20 minutes.2015101916 20 Mar 2015
- 9. The use according to any one of the claims 1 to 8, wherein when compared with the raw water of drinking water, the ultraviolet absorption peak of the obtained multi-polar micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably 28 nm to 30 nm, and most preferably 30 nm or 28 nm.
- 10. The use according to any one of the claims 1 to 9, wherein the beverage is a functional beverage and can be used for reducing blood urea;preferably, the health care products or medicaments can be used for reducing blood urea.
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