CN104720058A - Application of multi-pole micro-kinetic-energy drinking water in preparation of beverage, health product or drug for relaxing bowels - Google Patents
Application of multi-pole micro-kinetic-energy drinking water in preparation of beverage, health product or drug for relaxing bowels Download PDFInfo
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- CN104720058A CN104720058A CN201510125759.7A CN201510125759A CN104720058A CN 104720058 A CN104720058 A CN 104720058A CN 201510125759 A CN201510125759 A CN 201510125759A CN 104720058 A CN104720058 A CN 104720058A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K33/00—Medicinal preparations containing inorganic active ingredients
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/02—Medicinal preparations containing materials or reaction products thereof with undetermined constitution from inanimate materials
- A61K35/08—Mineral waters; Sea water
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0002—Galenical forms characterised by the drug release technique; Application systems commanded by energy
- A61K9/0009—Galenical forms characterised by the drug release technique; Application systems commanded by energy involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Inorganic Chemistry (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to application of multi-pole micro-kinetic-energy drinking water to preparation of a beverage, a health product or a drug for relaxing bowels. The multi-pole micro-kinetic-energy drinking water is prepared from raw drinking water in an electromagnetic wave non-contact manner; compared with the raw drinking water, the ultraviolet absorption peak is deviated for 25nm-40nm towards a short wave direction; preferably, the ultraviolet absorption peak is deviated for 28nm-30nm towards the short wave direction; and most preferably, the ultraviolet absorption peak is deviated for 30nm or 28nm towards the short wave direction. According to the application, the water is treated by the electromagnetic wave non-contact manner and any additive is not added; the preparation is simple, the production efficiency is high and the cost is low; and the prepared multi-pole micro-kinetic-energy drinking water has more extranuclear electrons which are at a high-energy-grade non-radiation energy grade state.
Description
Technical field
The invention belongs to water-treatment technology field, be specifically related to a kind of multipole fine motion energy drinking water for the preparation of the purposes in the beverage of defaecation, health products or medicine.
Background technology
Water (H
2o) inorganic matter be made up of hydrogen, oxygen two kinds of elements is the transparency liquid of colorless and odorless at normal temperatures and pressures.Water is one of modal material, and being the valuable source comprising all life existence of the mankind, is also the most important part of organism.Water plays an important role in Life Evolution.The mankind start to create understanding to water very early, ancient times between east and west simplicity view of matter in all water is considered as a kind of basic component.
Modern has following understanding with the angle of science to water:
Hydrone has memory: when scientist the snow crystals individually with difformity and weight dissolved respectively then freeze again time, find shape and the weight of the snow crystals again freezed and dissolve front just the same, obtaining the conclusion that water outlet itself has " memory ".
Due to the planform that hydrone itself has it special, it be by two hydrogen and oxonium ion with 104.5 angle be combined into.It is a kind of ionizing system with electronics " magnetic polarity ".Hydrone presents solid-state crystalline solid at zero degree and subzero, and we are referred to as ice.But water is not present aqueous completely at the temperature of zero to 60 DEG C, but exists with a kind of form of liquid crystal.If but temperature is higher than 60 DEG C, this liquid lens element just can wreck.
Due to the special angle mode of hydrone, the body of diluted solute molecule can be included in its liquid crystal, Here it is general understood course of dissolution.Present new discovery is, when by some means or other making solute molecule be separated with hydrone (as via dilution repeatedly and concussion), hydrone still can keep remembering " shape " of solute molecule.When body cell touches such hydrone, " shape " of this solute molecule memory can be used as genuine solute molecule and equally treat, this is the hahnemannian foundation stone having more than 200 year history.
Water is combined into by hydrogen and oxygen two kinds of elements, but is not present in nature with single hydrone, but is agglomerated into the group (H of hydrone by many hydrones
2o)
nbe present in nature (namely above-mentioned liquid crystals), the group of hydrone may be chain, ring-type, bulk or botryoidalis, minimum five hydrones, at most tens, tens of to hundreds of.Have at present and adopted electromagnetic wave to process hydrone, with the method improving the energy grade of hydrone or sterilization or prevention and take out stains.But these processing methods all can not the effect of control treatment well, and the effect of water after process is only limitted to drink mostly, does not have other additional advantageous effects.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of multipole fine motion energy drinking water adopting the contactless process of electromagnetic wave to obtain and its production and use.
An object of the present invention is to provide a kind of multipole fine motion energy drinking water.Another object of the present invention is to provide a kind of preparation method of above-mentioned multipole fine motion energy drinking water.Another object of the present invention is to provide a kind of purposes of above-mentioned multipole fine motion energy drinking water.
Above-mentioned purpose of the present invention adopts following technical scheme to realize.
On the one hand, the invention provides a kind of multipole fine motion energy drinking water, this multipole fine motion energy drinking water is prepared from by the contactless process of electromagnetic wave by Raw Drinking Water, compared with Raw Drinking Water, ultraviolet absorption peak is to shortwave direction skew 25nm-40nm, preferably to shortwave direction skew 28nm-30nm, most preferably to shortwave direction skew 30nm or 28nm.
Compared with Raw Drinking Water, the fluorescence emission spectrum intensity of multipole fine motion energy drinking water of the present invention all has at 300nm-400nm spectral coverage and significantly promotes, and repeat experiment to sample of the same race and show, this experiment has good repeatability.
Experiment shows, multipole fine motion energy drinking water of the present invention has more electron outside nucleus and is in the non-radiative energy level state of high level.
Preferably, described Raw Drinking Water is ordinary tap water or various mineral water or pure water, such as Evian natural mineral water, Glacier in Tibet mineral water, NongFuShanQuan, Wahaha etc.
Multipole fine motion energy drinking water of the present invention is drunk in 72 hours or result of use the best after preparing.
On the other hand, the invention provides a kind of preparation method of above-mentioned multipole fine motion energy drinking water, this preparation method comprises the former water of the contactless Drinking Water of electromagnetic wave adopting basic, normal, high three kinds of different frequencies, wherein, the frequency range of low-frequency electromagnetic wave is 30-100kHz, intermediate frequency electromagnetic wave frequency scope is 550-720kHz, and the frequency range of frequency electromagnetic waves is 300-725MHz.
Preferably, described Raw Drinking Water is ordinary tap water or various mineral water or pure water, such as Evian natural mineral water, Glacier in Tibet mineral water, NongFuShanQuan, Wahaha etc.
Preferably, the frequency range of described low-frequency electromagnetic wave is 45-75kHz.
Preferably, described intermediate frequency electromagnetic wave frequency scope is 600-720kHz.
Preferably, the frequency range of described frequency electromagnetic waves is 300-425MHz.
Preferably, described low-frequency electromagnetic wave and medium frequency electric magnetic wave are sine wave, square wave, sharp wave, sawtooth waveforms or trapezoidal wave.
Preferably, described low-frequency electromagnetic wave can be identical or different with intermediate frequency electromagnetic waveform, preferably identical.
Preferably, described low-frequency electromagnetic wave is consistent with intermediate frequency electromagnetic direction of wave travel.
Preferably, described frequency electromagnetic waves is triangular wave, and vertical with intermediate frequency electromagnetic direction of wave travel with described low-frequency electromagnetic wave.
Preferably, when adopting the contactless Drinking Water original sub-block of the electromagnetic wave of basic, normal, high three kinds of different frequencies, first use low-frequency electromagnetic wave and frequency electromagnetic waves to process simultaneously, and then use medium frequency electric magnetic wave and frequency electromagnetic waves to process simultaneously.
Preferably, the time using low-frequency electromagnetic wave and frequency electromagnetic waves simultaneously to process is 10-30 minute, is preferably 10-20 minute; The time using medium frequency electric magnetic wave and frequency electromagnetic waves simultaneously to process is 10-30 minute, is preferably 10-20 minute.
Preferably, compared with Raw Drinking Water, the ultraviolet absorption peak of the multipole fine motion energy drinking water prepared, to shortwave direction skew 25nm-40nm, preferably to shortwave direction skew 28nm-30nm, most preferably offsets 30nm or 28nm to shortwave direction.
Compared with Raw Drinking Water, the fluorescence emission spectrum intensity of multipole fine motion energy drinking water of the present invention all has at 300nm-400nm spectral coverage and significantly promotes, and repeat experiment to sample of the same race and show, this experiment has good repeatability.
Experiment shows, multipole fine motion energy drinking water of the present invention has more electron outside nucleus and is in the non-radiative energy level state of high level.
In the present invention, the method generated electromagnetic waves and device are ordinary skill in the art means.
Another aspect, the invention provides the purposes of above-mentioned multipole fine motion energy drinking water in preparation various mineral water, pure water, beverage, health products or medicine.
Preferably, described beverage is drinks, may be used for antifatigue, fat-reducing, defaecation, hypotensive, hypoglycemic, blood uric acid and plasma wrea fall.
Preferably, described health products or medicine may be used for antifatigue, fat-reducing, defaecation, hypotensive, hypoglycemic, fall blood uric acid and fall plasma wrea.
Compared with prior art, the present invention adopts the contactless process water of electromagnetic wave, does not add any additive, and make simple, production efficiency is high, and cost is low.
Compared with existing report, multipole fine motion energy drinking water of the present invention Raw Drinking Water more before treatment creates obvious displacement at ultraviolet absorption peak to shortwave direction, fluorescence emission spectrum intensity all has at 300nm-400nm spectral coverage and significantly promotes, and more electron outside nucleus is in the non-radiative energy level state of high level.Zoopery is verified have antifatigue, fat-reducing, defaecation, hypotensive, hypoglycemic, fall blood uric acid and fall plasma wrea effect.
Accompanying drawing explanation
Below, describe embodiment of the present invention in detail by reference to the accompanying drawings, wherein:
Fig. 1 is the water of different disposal group causes DNA damage impact on peroxide;
Fig. 2 is the impact of culture medium on cell of different disposal;
Fig. 3 is the absorption spectrum distribution of the multipole fine motion energy water of the present invention that the former water of common drinking water and the former water of this common drinking water of use obtain;
Fig. 4 is the absorption spectrum distribution of the multipole fine motion energy water of the present invention that Evian natural mineral water (former water) and this Evian natural mineral water of use obtain;
Fig. 5 is the absorption spectrum distribution of the multipole fine motion energy water of the present invention that Glacier in Tibet mineral water (former water) and this Glacier in Tibet mineral water of use obtain;
Fig. 6 is the fluorescence stable state transient test result of the multipole fine motion energy drinking water that common drinking water (former water) is made with it;
The fluorescence stable state transient test result of multipole fine motion energy drinking water that Fig. 7 is Evian natural mineral water (former water) and makes with it;
The fluorescence stable state transient test result of multipole fine motion energy drinking water that Fig. 8 is Glacier in Tibet mineral water (former water) and makes with it;
To be Glacier in Tibet mineral water be Fig. 9 that multipole fine motion energy drinking water that former water is made gets three samples respectively carries out reperformance test result;
Figure 10 is that multipole fine motion energy drinking water of the present invention is on the impact of mouse small intestine wriggling inhibition;
Figure 11 is that multipole fine motion energy drinking water of the present invention is on the impact of Constipated mice first grain melena defecation time;
Figure 12 is that multipole fine motion energy drinking water of the present invention is on the impact of Constipated mice stool weight;
Figure 13 is that multipole fine motion energy drinking water of the present invention is on the impact of Mouse Weight;
Figure 14 is that multipole fine motion energy drinking water of the present invention is on the impact of Constipated mice first grain melena drain time;
Figure 15 is that multipole fine motion energy drinking water of the present invention is to Constipated mice defecation particles effect;
Figure 16 is that multipole fine motion energy drinking water of the present invention is on the impact of Constipated mice defecation weight.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further described in detail, the embodiment provided only in order to illustrate the present invention, instead of in order to limit the scope of the invention.
Experimental technique in following embodiment, if no special instructions, is conventional method.Medicinal raw material used in following embodiment, reagent material etc., if no special instructions, be commercially available purchase product.
the preparation method of embodiment 1 multipole fine motion of the present invention energy drinking water
Outside common drinking water raw water tubes, the electromagnetic wave of the basic, normal, high three kinds of different frequencies of contactless applying processes, and wherein, low frequency electromagnetic wave frequency is 30kHz, intermediate frequency electromagnetic wave frequency scope is 550kHz, and the frequency range of frequency electromagnetic waves is 300MHz.
Wherein, described low-frequency electromagnetic wave and medium frequency electric magnetic wave are sine wave, and the two Electromagnetic Wave Propagation direction is consistent.Described frequency electromagnetic waves is triangular wave, and vertical with intermediate frequency electromagnetic direction of wave travel with described low-frequency electromagnetic wave.During process, first use low-frequency electromagnetic wave and frequency electromagnetic waves to process 20 minutes simultaneously, and then use medium frequency electric magnetic wave and frequency electromagnetic waves to process 20 minutes simultaneously.After having processed, obtain multipole fine motion energy drinking water of the present invention.
the preparation method of embodiment 2 multipole fine motion of the present invention energy drinking water
Outside common drinking water raw water tubes, the electromagnetic wave of the basic, normal, high three kinds of different frequencies of contactless applying processes, and wherein, low frequency electromagnetic wave frequency is 100kHz, intermediate frequency electromagnetic wave frequency scope is 720kHz, and the frequency range of frequency electromagnetic waves is 725MHz.
Wherein, described low-frequency electromagnetic wave and medium frequency electric magnetic wave are square wave, and the two Electromagnetic Wave Propagation direction is consistent.Described frequency electromagnetic waves is triangular wave, and vertical with intermediate frequency electromagnetic direction of wave travel with described low-frequency electromagnetic wave.During process, first use low-frequency electromagnetic wave and frequency electromagnetic waves to process 10 minutes simultaneously, and then use medium frequency electric magnetic wave and frequency electromagnetic waves to process 10 minutes simultaneously.After having processed, obtain multipole fine motion energy drinking water of the present invention.
the preparation method of embodiment 3 multipole fine motion of the present invention energy drinking water
Outside common drinking water raw water tubes, the electromagnetic wave of the basic, normal, high three kinds of different frequencies of contactless applying processes, and wherein, low frequency electromagnetic wave frequency is 45kHz, intermediate frequency electromagnetic wave frequency scope is 600kHz, and the frequency range of frequency electromagnetic waves is 300MHz.
Wherein, described low-frequency electromagnetic wave and medium frequency electric magnetic wave are square wave, and the two Electromagnetic Wave Propagation direction is consistent.Described frequency electromagnetic waves is triangular wave, and vertical with intermediate frequency electromagnetic direction of wave travel with described low-frequency electromagnetic wave.During process, first use low-frequency electromagnetic wave and frequency electromagnetic waves to process 15 minutes simultaneously, and then use medium frequency electric magnetic wave and frequency electromagnetic waves to process 15 minutes simultaneously.After having processed, obtain multipole fine motion energy drinking water of the present invention.
the preparation method of embodiment 4 multipole fine motion of the present invention energy drinking water
Outside common drinking water raw water tubes, the electromagnetic wave of the basic, normal, high three kinds of different frequencies of contactless applying processes, and wherein, low frequency electromagnetic wave frequency is 75kHz, intermediate frequency electromagnetic wave frequency scope is 720kHz, and the frequency range of frequency electromagnetic waves is 425MHz.
Wherein, described low-frequency electromagnetic wave and medium frequency electric magnetic wave are sharp wave, and the two Electromagnetic Wave Propagation direction is consistent.Described frequency electromagnetic waves is triangular wave, and vertical with intermediate frequency electromagnetic direction of wave travel with described low-frequency electromagnetic wave.During process, first use low-frequency electromagnetic wave and frequency electromagnetic waves to process 20 minutes simultaneously, and then use medium frequency electric magnetic wave and frequency electromagnetic waves to process 20 minutes simultaneously.After having processed, obtain multipole fine motion energy drinking water of the present invention.
the physical and chemical index of embodiment 5 multipole fine motion of the present invention energy drinking water detects
Detect multipole fine motion energy drinking water prepared by embodiment 1-4 according to " the drinking water standard method of inspection " (GB/T 5750-2006), testing result is basically identical, the results are shown in Table shown in 1.
The physical and chemical index testing result of table 1 multipole fine motion of the present invention energy drinking water
According to upper table, the index of fine motion energy drinking water of the present invention all meets the requirement of " standards for drinking water quality " (GB5749-2006).
the toxicological experiment of embodiment 6 multipole fine motion of the present invention energy drinking water
One, acute oral toxicity test
1, materials and methods
1.1 tested materials: fine motion energy drinking water 1.2 animal prepared by embodiment 3: provide Kun Ming mice (quality certification number: SCXK (capital) 2009-0017) by Laboratory Animal Resource research institute of National Institute for Food and Drugs Control, SPF level, body weight 18.0 ~ 22.0g, before test, animal overnight fasting, freely drinks water.
1.3 dosage groupings: tested material sets dosage as 20000mg/kg body weight, each 10 of female, buck.Cage group feeding is divided by sex.Adopt a per os gavage mode, calculate contamination amount by 0.1ml/10g body weight.
1.4 observation index: after contamination, observe the general status of animal, poisoning sign and death condition, observe two weeks time limits.During off-test, all animals are dissected, and the general pathology of record animal changes.Acute toxicity classification is carried out according to acute toxicity grading criteria.
2, result of the test
Tested material is to chmice acute Oral toxicity result of the test
3, conclusion
After contamination, animal occurs without any poisoning symptom and death condition, and have no any general pathology and change, the weight of animals is increase trend.Therefore, this tested material is to female, male small white mouse per os LD
50all be greater than 10000mg/kg body weight, belong to actual nontoxic level.
Two, DNA damage impact is caused on peroxide
1, experiment material and equipment
1.1 5mM vitamin c solutions: be dissolved in tri-distilled water, matching while using, vitamin C powder is provided by Sigma-Aldrich company;
1.2 5mM CuCl
2solution: be dissolved in tri-distilled water, matching while using, CuCl
2powder is provided by Chemical Reagent Co., Ltd., Sinopharm Group;
1.3 DNAs: pET28a plasmid increases extraction (plasmid extraction kit is purchased from Promega company) in DH5a Escherichia coli;
1.4 5mMEDTA pH value of solution 8.0:EDTA are provided by Chemical Reagent Co., Ltd., Sinopharm Group;
1.5 10 × PBS buffer solution: 80gNaCl, 2gKCl, 14.4gNa
2hPO
4, 2.4gKH
2pO
4be dissolved in distilled water, adjust pH to 7.4 is also settled to 1L autoclaving, and required reagent is all purchased from Chemical Reagent Co., Ltd., Sinopharm Group;
1.6 agaroses: provided by Biowest company;
1.7 Goldview nucleic acid dyes are provided by Biotium company;
1.8 cohesion imagers: Science and Technology Ltd. provides by the foundation of match intelligence;
1.9 electrophoresis apparatuses: 61 instrument plants;
1.10 tested materials: fine motion energy drinking water prepared by embodiment 3 and 4
2, experimental technique
2.1 experiment groupings
I group: untreated fish group, adopts untreated sterilized water to add reaction system;
II group: fine motion energy drinking water prepared by embodiment 3;
III group: fine motion energy drinking water prepared by embodiment 4.
Add each reactive material according to following order, reaction system final volume is 10 μ L:
10 × PBS buffer solution 1 μ L;
DNA (2 μ g) 2 μ L;
5mM vitamin C 1 μ L;
5mM CuC1
21μL；
With untreated water or process water polishing to 10 μ L.
2.2, respectively at 0.5 hour and l hour, add l μ L 5mM EDTA and 2.2 μ L sample-loading buffer cessation reactions;
2.3 0.8% agar chaff gel (containing Goldview dyestuff) electrophoresis l h;
2.4 gel imaging.
3, experimental result
Vitamin C and bivalent cupric ion generation redox reaction, along with the generation of peroxide, peroxide can cause Damage and Fracture to DNA, is embodied in weakening of electrophoretic band.As shown in Figure 1, experiment is divided into 3 groups to this experimental result, describes, every group reaction two time points: 0.5 hour and 1 hour, the brightness representation DNA content of DNA in the square case of group technology.Experimentally result display, II group, III group are compared with untreated I group, and the speed of DNA oxidated reduction reaction degraded is basically identical, does not produce the effect stoping or accelerate DNA damage fracture because of the process of water.
4, interpretation of result
Vitamin C and bivalent cupric ion generation redox reaction, along with the generation of peroxide, peroxide can cause Damage and Fracture to DNA, is embodied in weakening of electrophoretic band.This experimental result shows, and II group, III group are compared with untreated I group, and the speed of DNA oxidated reduction reaction degraded is basically identical, and because of the process of water, industry does not stop or accelerates the effect of DNA damage fracture.Illustrate that multipole fine motion energy water of the present invention causes DNA damage without impact to peroxide.
Three, in vitro culture HepG2 cell membrane integrity is affected
1, experiment material and equipment
L.1 human liver cancer cell: HepG2;
1.2 cell culture mediums: DMEM nutrient solution+10%FBS;
1.3 FAM-aptamer: purchased from Shanghai Sheng Gong bioengineering Co., Ltd;
1.4 fluorescence EZ micro mirrors: OLYMPUS company;
1.5 96 porocyte culture plates;
2, experimental technique
2.1 by the cell of exponential phase, adds appropriate trypsinization.Adjustment cell concentration, is inoculated in 96 well culture plates with the amount of 4000 cells/well, incubated overnight;
The 2.2 method process 40 minutes (processed group) 5mL being adopted embodiment 3 containing the DMEM culture medium (culture medium A) of 10%FBS;
2.3 by 5mL containing the DMEM culture medium (all support base B) of 10%FBS away from electromagnetic wave source more than 2 meters process 40 minutes (untreated fish group) of embodiment 3;
The C6-8aptamer that 4 μ g FAM mark joins in 100 μ L culture medium A and culture medium B and mixes by 2.4 respectively;
The cell liquid of adhere-wall culture in 96 orifice plates to be replaced by culture medium A containing FAM-C6-8aptamer and culture medium B by 2.5,37 DEG C cultivate 30min after, wash once with PBS, fluorescence microscopy Microscopic observation.
3, experimental result
Result is as Fig. 2, and what difference is the cellular morphology in processed group and untreated fish group do not have, and be all cell white hair fluorescence, cell membrane is complete, does not observe the C6-8aptamer green fluorescence that obvious FAM marks in cell.
4, results and analysis
If cell exist punch; FAM mark C6-8aptamer can enter cell and with intracellular protein combination, fluoresced green after 530 wavelength excite.And result sees what difference the cell in processed group and untreated fish group does not have in figure, be all cell autofluorescence, illustrate that cell membrane is complete, multipole fine motion energy technology of the present invention on cellular morphology and cell membrane integrity without impact.
wide spectral absorptivity and the rate of absorbing UV of embodiment 7 multipole fine motion of the present invention energy drinking water become
change
1, experiment material and equipment
Fine motion energy drinking water prepared by 1.1 embodiments 3;
The former water of common drinking water used in 1.2 embodiments 3;
1.3 drinking water adopting the identical method process Evian natural mineral water of embodiment 3 to make;
1.4 Evian natural mineral waters: buy in Carrefour hypermarket;
1.5 drinking water adopting the identical method process Glacier in Tibet mineral water of embodiment 3 to make;
1.6 Glacier in Tibet mineral waters: buy in Carrefour hypermarket;
1.7 ultra-pure waters: obtained by German Sartorius ultrapure water production system;
1.8 fiber spectrometer Ava-Spec3648, measurement category 200-1100nm, uncertainty/degree of accuracy 0.5nm;
1.9 oscillograph MS04104, equipment factory number: C001163, certainty of measurement: 1GHz;
1.10 photodetector Thorlab DET10A/M, equipment factory number: JGZX-ZXZC-012, certainty of measurement: 200nm-1100nm;
1.11 test conditions: temperature 21 DEG C, humidity: 38%.
2, experimental technique
The absorptivity of 2.1 wide spectral
(1) about 200ml ultrapure water experiment vessel are used to stablize to every physical and chemical index.
(2) fine motion energy drinking water prepared by the former water of common drinking water used in embodiment 3 and embodiment 3 is placed in experimental ware, the absorption of scanning 200nm ~ 800nm sample standard light source light spectrum light splitting, record experimental result.
(3) about 200ml ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
(4) Evian natural mineral water is placed in experimental ware with the drinking water adopting the identical method process Evian natural mineral water of embodiment 3 to make, repeats step (2), uitraviolet intensity variable quantity during record.
(5) about 200ml ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
(6) Glacier in Tibet mineral water is placed in experimental ware with the drinking water adopting the identical method process Glacier in Tibet mineral water of embodiment 3 to make, repeats step (2), uitraviolet intensity variable quantity during record.
(7) about 200ml ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
2.2 rate of absorbing UVs measure
(1) about 200ml ultrapure water experiment vessel are used to stablize to every physical and chemical index.
(2) fine motion energy drinking water embodiment 3 prepared is placed in uitraviolet intensity detection system, ultraviolet source irradiation water to be measured, the uitraviolet intensity numerical value of photodetector ThorlabDET10A/M reading without water to be measured is read by oscillograph MS04104, read the uitraviolet intensity numerical value through water to be measured, uninterrupted test 72 hours continuously, uitraviolet intensity variable quantity during record.
(3) about 200ml ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
(4) drinking water adopting the identical method process Evian natural mineral water of embodiment 3 to make is placed in uitraviolet intensity detection system, repeats step (2), uitraviolet intensity variable quantity during record.
(5) about 200ml ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
(6) drinking water adopting the identical method process Glacier in Tibet mineral water of embodiment 3 to make is placed in uitraviolet intensity detection system, repeats step (2), uitraviolet intensity variable quantity during record.
(7) about 200ml ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
3, experimental result
3.1 wide spectral absorption rate testings
The results are shown in Figure 3-5.
3.2 rate of absorbing UVs measure
The multipole fine motion energy drinking water that light water is made:
Rate of absorbing UV: 0.9135->0.9037
The multipole fine motion energy drinking water that Evian natural mineral water is made:
Rate of absorbing UV: 0.9244->0.9188
The multipole fine motion energy drinking water that Glacier in Tibet mineral water is made:
Rate of absorbing UV: 0.9157->0.9079
4, experiment conclusion and analysis
By above-mentioned experiment, obtain as drawn a conclusion:
(1) compare with former aqueous phase, the absorption spectrum absworption peak of multipole fine motion energy drinking water of the present invention is to shortwave skew about 30nm;
(2) under the test of 72 hours, the absorptivity of multipole fine motion energy drinking water of the present invention declines 1%.
the excited fluorescence spectrum change of embodiment 8 multipole fine motion of the present invention energy drinking water
1, experiment material and equipment
Fine motion energy drinking water prepared by 1.1 embodiments 3;
The former water of common drinking water used in 1.2 embodiments 3;
1.3 drinking water adopting the identical method process Evian natural mineral water of embodiment 3 to make;
1.4 Evian natural mineral waters: buy in Carrefour hypermarket;
1.5 drinking water adopting the identical method process Glacier in Tibet mineral water of embodiment 3 to make;
1.6 Glacier in Tibet mineral waters: buy in Carrefour hypermarket;
1.7 ultra-pure waters: obtained by German Sartorius ultrapure water production system;
1.8 fiber spectrometer Ava-Spec3648, measurement category 200-1100nm, uncertainty/degree of accuracy 0.5nm;
1.9 test environments: temperature 22 DEG C, humidity 37%.
2, experimental technique
(1) about 200mL ultrapure water experiment vessel are used to stablize to every physical and chemical index.
(2) fine motion energy drinking water embodiment 3 prepared is placed in fiber spectrometer sample cell, with 266nm pulsed ultraviolet laser to sample excitation pulse, use fiber spectrometer spectral scan function, to measure the emission spectrum of sample, the fluorescence emission spectrum of record sample.
(3) about 200mL ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
(4) the former water of common drinking water used in embodiment 3 is placed in fiber spectrometer sample cell, repeats step 2, record experimental data.
(5) about 200mL ultrapure water experiment vessel to every physical and chemical index is used to recover initial value and stablize.
(6) drinking water adopting the identical method process Evian natural mineral water of embodiment 3 to make is placed in optical spectrometer sample pond, repeats step 2,3, record experimental data.
(7) Evian natural mineral water is being placed in fiber spectrometer sample cell, is repeating step 2,3 record experimental datas.
(8) drinking water adopting the identical method process Glacier in Tibet mineral water of embodiment 3 to make is put in fiber spectrometer sample cell, repeat step 2,3 record experimental datas.
(9) Glacier in Tibet mineral water is placed in fiber spectrometer sample cell, repeats step 2,3 record experimental datas.
(10) drinking water adopting the identical method process Glacier in Tibet mineral water of embodiment 3 to make is got three samples and be placed in fiber spectrometer sample cell respectively, repeat step 2,3 record experimental datas.
3, experimental result
3.1 fluorescence stable state transient tests
The fluorescence stable state transient test of the multipole fine motion energy drinking water that common drinking water is made with it the results are shown in Figure 6.Evian natural mineral water and the fluorescence stable state transient test of multipole fine motion energy drinking water made with it the results are shown in Figure 7.Glacier in Tibet mineral water and the fluorescence stable state transient test of multipole fine motion energy drinking water made with it the results are shown in Figure 8.Glacier in Tibet mineral water is that multipole fine motion energy drinking water that former water is made is got three samples respectively and carried out reperformance test, the results are shown in Figure 9.
4, experiment conclusion and analysis
As can be seen from above-mentioned experimental result, compare with former aqueous phase, the fluorescence emission spectrum intensity of three kinds of different samples of multipole fine motion energy drinking water of the present invention all has at 300nm-400nm spectral coverage and significantly promotes, and repeat experiment to sample of the same race and show, this experiment has good repeatability.
Experiment shows, multipole fine motion energy water drinking water of the present invention has more electron outside nucleus and is in the non-radiative energy level state of high level.
the bowel relaxing functions of embodiment 9 multipole fine motion of the present invention energy drinking water: mouse small intestine is wriggled and presses down
the impact of simulation and the impact on Constipated mice defecation time, fecal grains and stool weight
1. materials and methods
1.1 samples:
Multipole fine motion energy drinking water (kinetic energy water) prepared by embodiment 3, the transparent aqueous liquid of colorless and odorless, normal temperature is preserved, and prepares in rear 72h and uses;
The logical tea of the logical profit of positive drug one glass, is produced by Bei Tong Co., Ltd of Shantou City one, lot number 20140206.
1.2 animals used as test: ICR mouse, purchased from Beijing HFK Bio-Technology Co., Ltd., production licence number: SCXK (capital) 2007-0001, the Quality of Experimental Animals quality certification number: 11401300009361.Totally 120, each experiment 60, male, often organize 12, totally 5 groups, order body weight 18-20g.
Rearing conditions: Laboratory Animal Facility continues to keep barrier environment standard.The control range of main environment index: room temperature 20 ~ 26 DEG C, temperature difference per day≤4 DEG C.Relative humidity 40 ~ 70%.Minimum air changes 15 times/hour, optical illumination: dark=12h:12h.Animal feeding is in polypropylene rat group support box, and cage tool specification is: 545*395*200mm
3, 5, every box, its cultured space meets the regulation about minimum space needed for animal used as test in National Standard of the People's Republic of China GB14925-2010.All animals carry out feeding and management by the qualified personnel of training.Change 1 bedding and padding and cage tool weekly.Add mouse special feed every day for animal edible, in whole feeding process, keep animal diet followed freedom of movement.
Animal welfare: this tests animal used and the requirement disposed and should meet animal welfare of being correlated with, and experiment is carried out will through the examination and approval of the Chinese Academy of Medical Sciences Beijing Union Medical College new drug Research on Safety Assessment central animals management and committee (IACUC).Concrete points for attention are as follows:
(1), in experimentation, the terrified of animal and pain should be reduced to a minimum.
(2) performing the operation to animal used as test, when dissected, must effectively anaesthetize.The convalescence after Miles operation according to actual conditions, should carry out analgesia and nurses targetedly and dietetic regulation.
(3), during the animal used as test of Baoding, should avoid causing the uneasiness of animal, terrified, pain and damage.Restraint appliance should rational in infrastructure, specification suitable, sturdy and durable, environmentally-friendly sanitary, convenient operation.Under the prerequisite not affecting experiment, should be reduced to a minimum to the mandatory restriction of animal bodies.
(4) sample collection: the operation in the mode of safety humanity (as far as possible reduce cause animal anxiety with uncomfortable), animal used as test being carried out to collecting sample.
(5) for the animal that moribund animals, infected animal and toxic reaction are serious, notify at once to animal doctor and thematic director, give treatment in time or implement euthanasia.When not affecting experimental result and judging, select " kind terminal ", avoid extending animal and bear the painful time.When putting to death animal used as test, implement mercy killing according to principle of humanitarianism.Put to death on-the-spot, on the scene without other animals.After confirming animal dead, properly dispose corpse.
(6) employment security aspect: in experimentation, notes taking personal protective measures, as gloves, barrier gown, face shield, eyeshade, earplug etc.
Each test is divided into 5 groups: blank group, model control group drink normal drinking water (gavage normal drinking water 0.3mL/10g body weight every day) every day; Kinetic energy water low dose group drinks kinetic energy water (the filling kinetic energy water of drinking bottle, changes 1 every day) every day; Kinetic energy water high dose group except kinetic energy water is drunk on basis every day, every day gavage kinetic energy water once, dosage is 0.3mL/10g body weight; Logical tea group gavage every day of the logical profit of positive drug one glass once, dosage is 3.3g/kg, administration volume is 0.3mL/10g body weight (profit logical tea every day 3 bags, amount to 6.6g, body weight for humans calculates with 60kg, amounts to 0.11g/kg, mouse is with 30 times of clinical people's consumption calculating, amount to 3.3g/kg, administration volume is 30mL/kg, and Pharmaceutical formulations concentration is 0.11g/mL).Experimental session prohibits water requirement without fasting.Successive administration 10 days.
1.4 key instruments and reagent: operating scissors, ophthalmic tweezers, ruler, syringe, balance, activated carbon powder, gum arabic, compound diphenoxylate, timer.
1.5 test methods:
Intestinal motility is tested: before formal experiment, first carry out preliminary experiment, determine to make mouse small intestine propelling rate compared with normal mouse obviously decline, and not too low compound diphenoxylate dosage (5mg/kg body weight), formally tests with this dosage.
To given the test agent after 10 days, the fasting of each group mouse can't help water 16 hours.
Model control group and three dosage group gavages give corresponding compound diphenoxylate suspension, blank group feedwater gavage.Per os to give after 5mg/kg compound diphenoxylate 0.5 hour, and dosage component does not give the prepared Chinese ink (activated carbon powder containing 5%, 10% gum arabic) containing corresponding given the test agent, and blank and model control group are to prepared Chinese ink gavage.After 20 minutes, de-cervical vertebra puts to death animal immediately, opens abdominal cavity and is separated mesenterium, clip upper end from pylorus, lower end to the intestinal tube of ileocecus, be placed on pallet, gently small intestine is pulled into straight line, measuring Length of intestine is " total small intestinal length ", is " prepared Chinese ink propelling length " from pylorus to prepared Chinese ink forward position.Be calculated as follows ink progradation:
Defecation time is tested: before formal experiment, first carry out preliminary experiment, determine that mouse can be made to arrange first grain melena time compared with normal mouse obviously to extend, and don't the compound diphenoxylate dosage (10mg/kg body weight) more than 5-6 hour, formally test with this dosage.
To given the test agent after 10 days, the fasting of each group mouse can't help water 16 hours.
Blank group is to drinking water, and model control group and three administration group gavages give compound diphenoxylate.Per os gives after 10mg/kg compound diphenoxylate after 0.5 hour, blank group and model control group mouse prepared Chinese ink gavage, and dosage group gives the prepared Chinese ink containing given the test agent, and all single cage of animal is raised, and normal water is taken food.From filling prepared Chinese ink, record every animal first grain row's melena time, record in 5 hours and arrange melena grain number and weight.
1.6 test data statistics:
Use SPSS statistical software process body weight result and carry out the comparative analysis of administration group and control group.Add up in accordance with the following methods: Kolmogorov-Smirnov method should be adopted to carry out Normal distribution test, Levene median method carries out homogeneity test of variance, if P > 0.05, carry out one-way analysis of variance (One-Way ANOVA) method, if normal state and homogeneity test of variance failure (P≤0.05), so need to carry out nonparametric Mann-Whitney inspection.
1.7 results judge:
Intestinal motility experimental result judges: under the prerequisite that model is set up, when the ink progradation of given the test agent group mouse is significantly higher than the ink progradation of model control group, can judge this experimental result positive.
The measurement result of defecation time, fecal grains and stool weight judges: under the prerequisite that small intestine Constipation Model is set up, and first grain row's melena time of given the test agent group mouse is significantly shorter than model control group, can judge that this index result is positive; Arrange melena grain number in 5 hours apparently higher than model control group, can judge that this index result is positive; Arrange melena weight in 5 hours apparently higher than model control group, can judge that this index result is positive.
Results of animal judges: arrange stool weight and any one of the fecal grains result positive in 5 hours, and intestinal motility experiment simultaneously and any one of the defecation time result positive, can judge this experimental result positive.
2. result:
Table 2 kinetic energy water on the impact of mouse small intestine wriggling inhibition, individual data items (%)
Table 3 kinetic energy water on the impact of mouse small intestine wriggling inhibition (ink progradation, %,
n=12)
Note:
##, compare with blank, p<0.01; *, compare with model comparison, p<0.05
From table 2, table 3, Figure 10, compare with blank group, model control group ink progradation significantly reduces (0.40 ± 0.15**, vs 0.81 ± 0.14, %, * * p<0.01), show mouse small intestine wriggling inhibition modeling success.
The ink progradation of kinetic energy water low dose group compares with model control group and has no significant difference, but absolute value increases to some extent, kinetic energy water high dose group ink progradation absolute value obviously increase and have significant difference (0.48 ± 0.07,0.52* ± 0.12, vs 0.40 ± 0.15, %, * p<0.05), show that kinetic energy water has the effect obviously promoting small bowel peristalsis to mouse small intestine wriggling inhibition in this experiment.
The logical tea group of positive drug profit compares ink progradation with model control group obviously increase (0.53 ± 0.10*, vs 0.81 ± 0.14, %, * p<0.05), shows that this test model is reliable.
Table 4 kinetic energy water on the impact of Constipated mice first grain melena defecation time, individual data items (min)
Table 5 kinetic energy water on the impact of Constipated mice first grain melena defecation time (min,
n=12)
Note: ##, compares with blank, p<0.01; *, compare with model comparison, p<0.05
From table 4, table 5, Figure 11, compare with blank group, the average defecation time of model control group first grain melena (151 ± 61**, vs 39 ± 33, min, * * p<0.01) significant prolongation.
Compare with model control group, kinetic energy water low dosage, the average defecation time of high dose group first grain melena (95 ± 57*, 84 ± 57*, vs 151 ± 61, min, * p<0.05) obviously shorten, show that kinetic energy water can promote the average defecation time of Constipated mice first grain melena.
Compare with model control group, the logical average defecation time of tea group first grain melena (79 ± 64*, the vs 151 ± 61, min of positive drug profit, * p<0.05) obviously shorten, show that the logical tea kinetic energy water of positive drug profit can promote the average defecation time of Constipated mice first grain melena.
Table 6 kinetic energy water on the impact of Constipated mice fecal grains, individual data items (grain)
Table 7 kinetic energy water on the impact of Constipated mice fecal grains (grain,
n=12)
Note:
##, compare with blank, p<0.01
From table 6, table 7, compare with blank group, model control group arranges melena grain number (5 ± 2 in 5 hours
##, vs 12 ± 5, grain,
##p<0.01 significantly reduces.
Compare with model control group, kinetic energy water low dosage, high dose group arrange melena grain number (6 ± 2,6 ± 2, vs 5 ± 2, grain, p > 0.05) absolute value in 5 hours to be increased to some extent, but has no significant difference.
Compare with model control group, the logical tea group of positive drug profit arranged melena grain number (7 ± 3, grain, p > 0.05) absolute value in 5 hours to be increased to some extent, but has no significant difference.
Table 8 kinetic energy water on the impact of Constipated mice stool weight, individual data items (g)
Table 9 kinetic energy water on the impact of Constipated mice stool weight (g,
n=12)
Note:
##, compare with blank, p<0.01; *, compare with model comparison, p<0.05
From table 8, table 9, Figure 12, compare with blank group, model control group arranged melena weight (0.081 ± 0.062 in 5 hours
##, vs 0.193 ± 0.101, g,
##p<0.01) significantly reduce.
Compare with model control group, kinetic energy water low dosage, high dose group arrange melena weight (0.136 ± 0.063*, 0.113 ± 0.083, vs 0.081 ± 0.062, g in 5 hours, * p<0.01) obviously increase, show that kinetic energy water can increase in 5 hours and arrange melena weight.
Compare with model control group, the logical tea group of the logical profit of positive drug one glass arranged melena weight (0.161 ± 0.098*, vs 0.081 ± 0.062, g in 5 hours, * p<0.01) obviously increase, show that the logical tea kinetic energy water of positive drug profit can increase in 5 hours and arrange melena weight.
3. brief summary
Under this experiment condition, kinetic energy water has the effect obviously promoting small bowel peristalsis to mouse small intestine wriggling inhibition.
Under this experiment condition, kinetic energy water can promote the average defecation time of Constipated mice first grain melena, arranges melena grain number and weight in increasing by 5 hours.
Comprehensive the above results, under this experiment condition, multipole fine motion energy drinking water of the present invention has bowel relaxing functions.
the bowel relaxing functions of embodiment 10 multipole fine motion of the present invention energy drinking water: mouse small intestine is wriggled
the impact of inhibition and the impact on Constipated mice defecation time, fecal grains and stool weight
1, material
Multipole fine motion energy drinking water (kinetic energy water) prepared by 1.1 embodiments 4, the transparent aqueous liquid of colorless and odorless, normal temperature is preserved, and prepares in rear 72h and uses;
1.2 8 week age male C57bl/6, purchased from dimension tonneau China animal used as test Technology Co., Ltd..
1.3 compound diphenoxylates, are produced by Hangzhou Kang Pu pharmaceutcal corporation, Ltd.
2, experimental procedure:
2.1 by 45 male 8 week age C57bl/6 mouse by body weight, be divided into 3 groups at random, often organize 15, be respectively:
Blank group: give normal drinking water and freely take food;
Model control group: give normal drinking water and freely take food;
Fine motion energy water group: give the multipole fine motion energy drinking water of embodiment 4 preparation and freely take food.
2.2 mouse at different levels gave corresponding drinking water after 10 days, carried out fasting 16 hours.
After 2.3 pairs of fasting, mouse carries out measured body weight, and give compound diphenoxylate by 10mg/kg body weight to model control group and fine motion energy water two groups of mouse, blank group then gives the normal drinking water of respective volume.
2.4 administration is after 30 minutes, prepared Chinese ink is carried out to each group of mouse and gavages that ight soil is marked.
2.5 pairs respectively group mouse first grain melena drain time, in 5 hours, the pm emission number of melena and weight are monitored.
2.6 pairs of number driving row analyses accordingly.
3, experimental result:
3.1 multipole fine motion energy drinking water of the present invention are on the impact of Mouse Weight
Multipole fine motion energy drinking water of the present invention affects result as shown in Figure 13, table 10,11 to Mouse Weight.
Table 10: multipole fine motion energy drinking water of the present invention is on the impact of mice weights (gram, x ± s.e., n=15)
| Blank group | Model control group | Fine motion energy water group | |
| Average weight (gram) | 21.11±0.315 | 21.03±0.322 | 20.37±0.316 |
Table 11: multipole fine motion energy drinking water of the present invention on the impact of mice weights, individual mouse data:
From Figure 13, table 10 and table 11, compared with blank group and model control group, average weight (20.37 ± 0.316vs21.11 ± 0.315 of fine motion energy water group, 21.03 ± 0.322 grams) without obviously changing, show to drink fine motion energy water in a short time to C57bl/6 Mouse Weight without impact.
3.2 multipole fine motion energy drinking water of the present invention are on the impact of Constipated mice defecation time
Multipole fine motion energy drinking water of the present invention affects result as shown in Figure 14, table 12,13 to Constipated mice defecation time.
Table 12: multipole fine motion energy drinking water of the present invention is on the impact of Constipated mice first grain melena drain time (minute, x ± s.e., n=15)
###, compared with blank group, P<0.001; *, compared with model control group, P<0.01.
Table 13: multipole fine motion energy drinking water of the present invention on the impact of Constipated mice first grain melena drain time, individual mouse data:
From Figure 14, table 12 and table 13, compared with blank group, the first grain melena drain time (194.5 ± 3.7 of model control group and fine motion energy water group
###, 170.7 ± 7.6
###vs 56.7 ± 5.1min;
###p<0.01) significant prolongation; But compared with model control group, fine motion energy water group first grain melena average emission time (170.7 ± 7.6**vs, 194.5 ± 3.7min; * P<0.01) significantly shorten, surperficial multipole fine motion energy drinking water of the present invention can promote the defaecation of mice with constipation model.
3.3 multipole fine motion energy drinking water of the present invention are on the impact of Constipated mice defecation particle
Multipole fine motion energy drinking water of the present invention affects result as shown in Figure 15, table 14, table 15 to Constipated mice defecation particle.
Table 14: multipole fine motion energy drinking water of the present invention is on the impact (grain, x ± s.e., n=15) of Constipated mice defecation particle
| Blank group | Model control group | Fine motion energy water group | |
| Excrement particles number (grain) | 34.33±1.14 | 19.60±1.90 ### | 21.27±1.13 ### |
###, compared with blank group, P<0.001
Table 15: multipole fine motion energy drinking water of the present invention on the impact of Constipated mice defecation particle, individual mouse data:
From Figure 15, table 14,15, compared with blank group, model control group and fine motion energy water group 5 hourly average defecation particle (19.60 ± 1.90
###, 21.27 ± 1.13
###vs 34.33 ± 1.14;
###p<0.001) significantly reduce; Compared with model control group, fine motion energy water group 5 hourly average defecation particle (21.27 ± 1.13vs 19.60 ± 1.90) average slightly increases but no difference of science of statistics.3.3 multipole fine motion energy drinking water of the present invention are on the impact of Constipated mice defecation particle
3.4 multipole fine motion energy drinking water of the present invention affect result as shown in Figure 16, table 16, table 17 to Constipated mice defecation weight.
Table 16: multipole fine motion energy drinking water of the present invention is on the impact of Constipated mice defecation weight (gram, x ± se., n=15)
| Blank group | Model control group | Fine motion energy water group | |
| Stool weight (gram) | 0.43±0.01 | 0.24±0.02 ### | 0.27±0.02 ### |
###, compared with blank group, P<0.001
Table 17: multipole fine motion energy drinking water of the present invention on the impact of Constipated mice defecation weight, individual mouse data:
From Figure 16, table 16, table 17, compared with blank group, model control group and fine motion energy water group 5 hourly average defecation weight (0.24 ± 0.02
###, 0.27 ± 0.02
###vs 0.43 ± 0.01;
###p<0.001) significantly decline; Compared with model control group, fine motion energy water group 5 hourly average defecation weight (0.27 ± 0.02vs 0.24 ± 0.02) average slightly increases but no difference of science of statistics.
4, brief summary
Under this experiment condition, drinking multipole fine motion energy drinking water of the present invention does not in a short time affect Mouse Weight.And multipole fine motion energy drinking water of the present invention, when not changing defecation particle and defecation body weight, effectively can shorten the drain time of the first grain melena of Constipated mice, have bowel relaxing functions.
Claims (10)
1. multipole fine motion energy drinking water is for the preparation of the purposes in the beverage of defaecation, health products or medicine.
2. purposes according to claim 1, it is characterized in that, described multipole fine motion energy drinking water adopts the former water of the contactless Drinking Water of electromagnetic wave of basic, normal, high three kinds of different frequencies to be prepared from, wherein, the frequency range of low-frequency electromagnetic wave is 30-100kHz, intermediate frequency electromagnetic wave frequency scope is 550-720kHz, and the frequency range of frequency electromagnetic waves is 300-725MHz.
3. purposes according to claim 1 and 2, is characterized in that, described Raw Drinking Water is ordinary tap water or various mineral water or pure water, such as Evian natural mineral water, Glacier in Tibet mineral water, NongFuShanQuan, Wahaha etc.
4. purposes according to any one of claim 1 to 3, is characterized in that, the frequency range of described low-frequency electromagnetic wave is 45-75kHz;
Preferably, described intermediate frequency electromagnetic wave frequency scope is 600-720kHz;
Preferably, the frequency range of described frequency electromagnetic waves is 300-425MHz.
5. purposes according to any one of claim 1 to 4, is characterized in that, described low-frequency electromagnetic wave and medium frequency electric magnetic wave are sine wave, square wave, sharp wave, sawtooth waveforms or trapezoidal wave;
Preferably, described low-frequency electromagnetic wave is identical with intermediate frequency electromagnetic waveform or different;
More preferably, described low-frequency electromagnetic wave is preferably identical with intermediate frequency electromagnetic waveform.
6. purposes according to any one of claim 1 to 5, it is characterized in that, described low-frequency electromagnetic wave is consistent with intermediate frequency electromagnetic direction of wave travel.
7. purposes according to any one of claim 1 to 6, described frequency electromagnetic waves is triangular wave, and vertical with intermediate frequency electromagnetic direction of wave travel with described low-frequency electromagnetic wave.
8. purposes according to any one of claim 1 to 7, it is characterized in that, when adopting the contactless Drinking Water original sub-block of the electromagnetic wave of basic, normal, high three kinds of different frequencies, first use low-frequency electromagnetic wave and frequency electromagnetic waves to process simultaneously, and then use medium frequency electric magnetic wave and frequency electromagnetic waves to process simultaneously;
Preferably, the time using low-frequency electromagnetic wave and frequency electromagnetic waves simultaneously to process is 10-30 minute, is preferably 10-20 minute;
More preferably, the time using medium frequency electric magnetic wave and frequency electromagnetic waves simultaneously to process is 10-30 minute, is preferably 10-20 minute.
9. purposes according to any one of claim 1 to 8, it is characterized in that, compared with Raw Drinking Water, the ultraviolet absorption peak of described multipole fine motion energy drinking water is to shortwave direction skew 25nm-40nm, preferably to shortwave direction skew 28nm-30nm, most preferably to shortwave direction skew 30nm or 28nm.
10. purposes according to any one of claim 1 to 9, is characterized in that, described beverage is drinks, may be used for defaecation;
Preferably, described health products or medicine may be used for defaecation.
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| WO2016149885A1 (en) * | 2015-03-20 | 2016-09-29 | 欢乐海（北京）水业科技有限公司 | Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament for reducing blood urea |
| WO2016149879A1 (en) * | 2015-03-20 | 2016-09-29 | 欢乐海（北京）水业科技有限公司 | Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament used for combating fatigue |
| WO2016149883A1 (en) * | 2015-03-20 | 2016-09-29 | 欢乐海（北京）水业科技有限公司 | Uses of multipolar microkinetic drinking water in preparing drink, healthcare or medicament used for reducing blood pressure |
| WO2016149872A1 (en) * | 2015-03-20 | 2016-09-29 | 欢乐海（北京）水业科技有限公司 | Multipolar microkinetic drinking water, preparation method for same, and uses thereof |
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| WO2016149886A1 (en) * | 2015-03-20 | 2016-09-29 | 欢乐海（北京）水业科技有限公司 | Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament for use in reducing blood urea |
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| WO2016149883A1 (en) * | 2015-03-20 | 2016-09-29 | 欢乐海（北京）水业科技有限公司 | Uses of multipolar microkinetic drinking water in preparing drink, healthcare or medicament used for reducing blood pressure |
| WO2016149872A1 (en) * | 2015-03-20 | 2016-09-29 | 欢乐海（北京）水业科技有限公司 | Multipolar microkinetic drinking water, preparation method for same, and uses thereof |
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