WO2016149879A1 - Uses of multipolar microkinetic drinking water in preparing drink, healthcare product or medicament used for combating fatigue - Google Patents

Abstract

Uses of a multipolar microkinetic drinking water in preparing a drink, healthcare product or medicament used for combating fatigue. The multipolar microkinetic drinking water is prepared from raw drinking water being passed through an electromagnetic wave without contact. Compared with the raw drinking water, the ultraviolet absorption peak is offset by 25 nm to 40 nm in the direction of shortwaves, where the preferred offset is 28 nm to 30 nm in the direction of the shortwaves, and the optimal offset is either 30 nm or 28 nm in the direction of the shortwaves.

Description

Use of multi-pole micro-kinetic drinking water in the preparation of beverages, health products or medicines for use in anti-fatigue Technical field

The invention belongs to the technical field of water treatment, and particularly relates to the use of multi-pole micro-kinetic energy drinking water in preparing beverages, health care products or medicines for anti-fatigue.

Background technique

Water (H ₂ O) is an inorganic substance composed of two elements of hydrogen and oxygen, and is a colorless and odorless transparent liquid under normal temperature and normal pressure. Water is one of the most common substances, an important resource for all life, including human beings, and the most important component of living things. Water plays an important role in the evolution of life. Humans have long begun to understand water, and water is regarded as a basic component in the ancient and simple material view of the East and West.

Modern people have the following understanding of water from a scientific perspective:

Water molecules have memory: When scientists melted individual snow crystals with different shapes and weights and then frozen them, it was found that the shape and weight of the re-frozen snow crystals were exactly the same as before melting, which concluded that the water itself had "memory". .

Since the water molecule itself has its special structural shape, it is composed of two hydrogen and one oxygen ion combined at an angle of 104.5. It is an ionization system with an electronic "magnetic polarity". Water molecules exhibit solid crystals below zero and below zero, which we call ice. However, water is not completely liquid at a temperature of from zero to 60 ° C, but exists in the form of a liquid crystal. However, if the temperature is higher than 60 ° C, the liquid crystal molecules will be destroyed.

Due to the special angle of the water molecules, the form of the diluted solute molecules can be contained in its liquid crystals, which is a generally understood dissolution process. The new discovery is that when a solute molecule is separated from a water molecule by some means (eg, through multiple dilutions and oscillations), the water molecule can still maintain a "shape" memory of the solute molecule. When the body cells are exposed to such water molecules, the "shape" memory of the solute molecule is treated as a true solute molecule, which is the cornerstone of homeopathic therapy with more than 200 years of history.

Water is composed of two elements of hydrogen and oxygen, but does not exist in nature as a single water molecule. Instead, a group of water molecules (H ₂ O) _{n is} present in nature (ie, the above liquid state). Crystallization), the group of water molecules may be chain, ring, agglomerate or grape-like, with at least five water molecules, and more than a dozen, tens to hundreds.

Electromagnetic waves have been used to treat water molecules to increase the energy level of water molecules or to disinfect or prevent and remove dirt. However, these treatment methods do not control the effect of the treatment well, and the effect of the treated water is mostly limited to drinking, and there is no other additional advantageous effect.

Summary of the invention

In order to overcome the defects of the prior art, the present invention provides a multi-pole micro-kinetic energy drinking water obtained by electromagnetic wave non-contact treatment, a preparation method thereof and use thereof.

It is an object of the present invention to provide a multi-pole micro-kinetic energy drinking water. Another object of the present invention is to provide a method of preparing the above-described multi-pole micro-kinetic energy drinking water. It is still another object of the present invention to provide a use of the above-described multi-pole micro-kinetic energy drinking water.

The above object of the present invention is achieved by the following technical solutions.

In one aspect, the present invention provides a multi-pole micro-kinetic energy drinking water prepared by non-contact treatment of drinking water raw water by electromagnetic waves, and the ultraviolet absorption peak is short-waved compared with the raw water of drinking water. The direction shift is 25 nm to 40 nm, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.

Compared with the raw water of drinking water, the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .

Experiments have shown that the multi-pole micro-kinetic energy drinking water of the present invention has more extra-nuclear electrons in a high-energy non-radiative energy level.

Preferably, the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Tibet glacier mineral water, Nongfu Spring, Wahaha, and the like.

The multi-pole micro-kinetic energy drinking water of the present invention is best consumed or used within 72 hours after preparation.

In another aspect, the present invention provides a method for preparing the above-mentioned multi-pole micro-kinetic energy drinking water, which comprises non-contact treatment of drinking water raw water by using electromagnetic waves of low, medium and high frequencies, wherein the frequency of low-frequency electromagnetic waves The range is 30-100 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz, and the frequency range of the high frequency electromagnetic wave is 300-725 MHz.

Preferably, the drinking water raw water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Tibet glacier mineral water, Nongfu Spring, Wahaha, and the like.

Preferably, the low frequency electromagnetic wave has a frequency in the range of 45-75 kHz.

Preferably, the intermediate frequency electromagnetic wave has a frequency range of 600-720 kHz.

Preferably, the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.

Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are a sine wave, a square wave, a sharp wave, a sawtooth wave or a trapezoidal wave.

Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform may be the same or different, preferably the same.

Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.

Preferably, the high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave.

Preferably, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by the electromagnetic wave non-contact treatment of the drinking water raw water treatment by using three different frequencies of low, medium and high frequencies, and then the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave are simultaneously processed.

Preferably, the time for simultaneous treatment using the low frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes; the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10-30 minutes, preferably 10-20 minutes. .

Preferably, the prepared ultraviolet light absorption peak of the multi-pole micro-kinetic energy drinking water is shifted to the short-wave direction by 25 nm to 40 nm, preferably to the short-wave direction by 28 nm to 30 nm, and most preferably to the short-wave direction. 30 nm or 28 nm.

Compared with the raw water of drinking water, the spectral intensity of the fluorescent radiation of the multi-pole micro-kinetic energy drinking water of the invention is greatly improved in the spectrum of 300 nm to 400 nm, and repeated experiments on the same sample show that the experiment has good repeatability. .

Experiments have shown that the multi-pole micro-kinetic energy drinking water of the present invention has more extra-nuclear electrons in a high-energy non-radiative energy level.

In the present invention, a method and apparatus for generating electromagnetic waves are conventional techniques in the art.

In still another aspect, the present invention provides the use of the above-described multi-pole micro-kinetic energy drinking water in the preparation of various mineral water, purified water, beverages, health care products or medicines.

Preferably, the beverage is a functional beverage and can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.

Preferably, the health care product or drug can be used for anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid lowering and blood urea lowering.

Compared with the prior art, the invention adopts electromagnetic wave non-contact type treatment water, without adding any additives, is simple to manufacture, has high production efficiency and low cost.

Compared with the existing reports, the multi-pole micro-kinetic energy drinking water of the present invention is more than the drinking water before treatment. The original water produced a significant shift in the ultraviolet absorption peak to the short-wave direction. The spectral intensity of the fluorescent radiation was greatly improved in the 300-400 nm spectrum, and more extranuclear electrons were in the high-energy non-radiative energy level. Animal experiments have proven to have anti-fatigue, weight loss, laxative, blood pressure lowering, blood sugar lowering, blood uric acid and blood urea.

DRAWINGS

Hereinafter, embodiments of the present invention will be described in detail with reference to the accompanying drawings, in which:

Figure 1 shows the effect of water in different treatment groups on peroxide-induced DNA damage;

Figure 2 shows the effect of medium in different treatment groups on cells;

3 is an absorption spectrum distribution of the ordinary drinking water raw water and the multi-pole micro kinetic energy of the present invention obtained by using the ordinary drinking water raw water;

4 is an absorption spectrum distribution of the multi-pole micro kinetic energy of the present invention obtained by Evian natural mineral water (raw water) and using the Evian natural mineral water;

5 is an absorption spectrum distribution of the glacial mineral water (raw water) of Tibet and the multi-pole micro kinetic energy of the present invention obtained by using the glacial mineral water of the Tibet;

Figure 6 is a fluorescence steady state transient test result of ordinary drinking water (raw water) and its multi-pole micro-kinetic energy drinking water;

Figure 7 is a fluorescence steady state transient test result of Evian natural mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;

Figure 8 shows the fluorescence steady-state transient test results of Tibetan glacial mineral water (raw water) and multi-pole micro-kinetic energy drinking water made therewith;

Figure 9 shows the results of repeatability test of three samples of multi-pole micro-kinetic energy drinking water made from raw water of Tibet glacial mineral water;

Figure 10 shows the effect of kinetic energy on the weight-bearing swimming time in mice.

detailed description

The present invention is further described in detail with reference to the preferred embodiments thereof.

The experimental methods in the following examples are conventional methods unless otherwise specified. The medicinal materials, reagent materials and the like used in the following examples are commercially available products unless otherwise specified.

Embodiment 1 Method for preparing multi-pole micro-kinetic energy drinking water of the present invention

In addition to ordinary drinking water raw water pipes, electromagnetic waves of three different frequencies of low, medium and high are applied in a non-contact manner, wherein the frequency of the low frequency electromagnetic wave is 30 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550 kHz, and the frequency range of the high frequency electromagnetic wave is 300 MHz. .

Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both sinusoidal waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.

Example 2 Preparation method of multi-pole micro-kinetic energy drinking water of the invention

In addition to ordinary drinking water raw water pipes, non-contact electromagnetic waves of low, medium and high frequencies are applied, wherein the frequency of the low frequency electromagnetic waves is 100 kHz, the frequency range of the intermediate frequency electromagnetic waves is 720 kHz, and the frequency range of the high frequency electromagnetic waves is 725 MHz. .

Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 10 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 10 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.

Example 3 Preparation method of multi-pole micro-kinetic energy drinking water of the invention

In addition to ordinary drinking water raw water pipes, electromagnetic waves of low, medium and high frequencies are applied non-contactly, wherein the frequency of the low frequency electromagnetic waves is 45 kHz, the frequency range of the intermediate frequency electromagnetic waves is 600 kHz, and the frequency range of the high frequency electromagnetic waves is 300 MHz. .

Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are both square waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 15 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 15 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.

Embodiment 4 Method for preparing multi-pole micro-kinetic energy drinking water of the present invention

In addition to ordinary drinking water raw water pipes, electromagnetic waves of three different frequencies of low, medium and high are applied in a non-contact manner, wherein the frequency of the low frequency electromagnetic waves is 75 kHz, and the frequency of the intermediate frequency electromagnetic waves is The frequency range is 720 kHz, and the frequency range of high frequency electromagnetic waves is 425 MHz.

Wherein, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sharp waves, and the electromagnetic wave propagation directions of the two are the same. The high frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave. When processing, the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes, and then the intermediate frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed for 20 minutes. After the treatment is completed, the multi-pole micro-kinetic energy drinking water of the present invention is obtained.

Example 5 Physical and chemical index detection of multi-pole micro-kinetic energy drinking water of the present invention

The multi-pole micro-kinetic energy drinking water prepared in Examples 1-4 was tested according to the Standard Test Method for Drinking Water (GB/T 5750-2006), and the test results were basically the same. The results are shown in Table 1.

Table 1 Physical and chemical indicators test results of multi-pole micro-kinetic energy drinking water of the present invention

According to the above table, the indicators of the micro-kinetic energy drinking water of the present invention all meet the requirements of the Sanitary Standard for Drinking Water (GB5749-2006).

Example 6 Toxicological experiment of multi-pole micro-kinetic energy drinking water of the present invention

I. Acute oral toxicity test

1. Materials and methods

1.1 Test substance: the micro-kinetic energy drinking water prepared in Example 3

1.2 Animals: Kunming mice were provided by the Laboratory Animal Resources Research Institute of China Food and Drug Control Research Institute (Qualification No.: SCXK (Beijing) 2009-0017), SPF grade, body weight 18.0-22.0g, animals were fasted overnight before the test. Free drinking water.

1.3 Dosage grouping: The test substance is set at a dose of 20,000 mg/kg body weight, and each of the female and male animals is 10. Grouped by sex. The dose was calculated by oral gavage at 0.1 ml/10 g body weight.

1.4 Observation indicators: After exposure, observe the general condition, poisoning signs and death of the animals, and observe the period of two weeks. At the end of the experiment, all animals were dissected and gross pathological changes in the animals were recorded. Acute toxicity grading according to acute toxicity grading standards.

2, test results

Acute oral toxicity test results of test subjects on mice

3. Conclusion

After the exposure, the animals did not show any symptoms of poisoning and death. No gross pathological changes were observed, and the body weight of the animals showed an increasing trend. Therefore, the oral LD _{50 of} the test article for both female and male mice is greater than 10000 mg/kg body weight, which is an actual non-toxic grade.

Second, the effect of peroxide-induced DNA damage

1. Experimental materials and equipment

1.1 5mM vitamin C solution: dissolved in three distilled water, ready to use, vitamin C powder provided by Sigma-Aldrich;

1.2 5mM CuCl ₂ solution: dissolved in three distilled water, ready to use, CuCl ₂ powder is provided by Sinopharm Chemical Reagent Co., Ltd.;

1.3 Plasmid DNA: pET28a plasmid was amplified and extracted in DH5a E. coli (plasmid extraction kit was purchased from Promega);

1.4 5mM EDTA solution pH 8.0: EDTA is provided by Sinopharm Chemical Reagent Co., Ltd.;

1.5 10×PBS buffer: 80g NaCl, 2gKCl, 14.4g Na ₂ HPO ₄ , 2.4g KH ₂ PO ₄ dissolved in distilled water, adjusted to pH 7.4 and fixed to 1L autoclave, the required reagents were purchased from Sinopharm Chemical Reagent Limited company;

1.6 agarose: supplied by Biowest;

1.7 Goldview nucleic acid dyes are supplied by Biotium;

1.8 Condensation Imager: provided by Saizhi Entrepreneur Technology Co., Ltd.;

1.9 Electrophoresis Instrument: Liuyi Instrument Factory;

1.10 Test substance: Micro-kinetic energy drinking water prepared in Examples 3 and 4

2, experimental methods

2.1 Experimental grouping

Group I: untreated group, adding untreated sterile water to the reaction system;

Group II: the kinetic energy drinking water prepared in Example 3;

Group III: The kinetic energy drinking water prepared in Example 4.

The reaction materials were added in the following order, and the final volume of the reaction system was 10 μL:

10×PBS buffer 1 μL;

Plasmid DNA (2 μg) 2 μL;

5 mM vitamin C 1 μL;

5 mM CuC1 ₂ 1 μL;

Add up to 10 μL with untreated or treated water.

2.2 The reaction was terminated by adding 1 μL of 5 mM EDTA and 2.2 μL of loading buffer at 0.5 hour and 1 hour, respectively.

2.3 0.8% agar 糠 gel (containing Goldview dye) electrophoresis l h;

2.4 Gel imaging.

3. Experimental results

The redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band. The results of this experiment are shown in Figure 1. The experiments were divided into three groups. The grouping method is described in the scheme. The two time points of each group were: 0.5 hours and 1 hour, and the brightness of DNA represented the DNA content. According to the experimental results, the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no effect of preventing or accelerating DNA damage and cleavage due to water treatment.

4, the result analysis

The redox reaction between vitamin C and divalent copper ions is accompanied by the formation of peroxide, which causes damage to the plasmid DNA, which is reflected in the weakening of the electrophoresis band. The results of this experiment showed that the rate of degradation of plasmid DNA by redox reaction was basically the same in group II and group III compared with untreated group I, and there was no industrial effect of preventing or accelerating DNA damage and cleavage due to water treatment. It is indicated that the multipolar micro kinetic energy of the present invention has no effect on peroxide-induced DNA damage.

3. Effect on membrane integrity of HepG2 cells cultured in vitro

1. Experimental materials and equipment

L.1 human liver cancer cells: HepG2;

1.2 cell culture medium: DMEM culture solution + 10% FBS;

1.3FAM-aptamer: purchased from Shanghai Shenggong Bioengineering Co., Ltd.;

1.4 Fluorescent EZ Micromirror: OLYMPUS;

1.5 96-well cell culture plate;

2, experimental methods

2.1 Add the appropriate amount of trypsin to the cells in the logarithmic growth phase. The cell concentration was adjusted, and the cells were seeded in a 96-well culture plate at 4000 cells/well, and cultured overnight;

2.2 5 mL of DMEM medium (medium A) containing 10% FBS was treated by the method of Example 3 for 40 minutes (treatment group);

2.3 5 mL of DMEM medium containing 10% FBS (homogeneous group B) was treated for more than 2 m from the electromagnetic wave source of Example 3 for 40 minutes (untreated group);

2.4 4 μg of FAM-labeled C6-8 aptamer was added to 100 μL of medium A and medium B, respectively;

2.5 The cell culture medium adherently cultured in a 96-well plate was changed to medium A and medium B containing FAM-C6-8 aptamer, cultured at 37 ° C for 30 min, washed once with PBS, and observed under a fluorescence microscope.

3. Experimental results

The results are shown in Fig. 2. There is no difference in cell morphology between the treated group and the untreated group. The cells are white-fluorescent, the cell membrane is intact, and no obvious FAM-labeled C6-8aptamer green fluorescence is observed in the cells.

4. Results and analysis

If the cells are punctured, the FAM-labeled C6-8 aptamer can enter the cell and bind to the protein inside the cell, and emit green fluorescence after excitation at 530 wavelength. The results in the figure show that there is no difference between the cells in the treated group and the untreated group, all of which are autofluorescence, indicating that the cell membrane is intact, and the multipolar microkinetic energy technology of the present invention has no effect on cell morphology and cell membrane integrity.

Example 7 Wide-spectrum absorption rate and ultraviolet absorption rate of multi-pole micro-kinetic energy drinking water of the present invention Variety

1. Experimental materials and equipment

1.1 The kinetic energy drinking water prepared in Example 3;

1.2 ordinary drinking water raw water used in embodiment 3;

1.3 treating the drinking water made of Evian natural mineral water in the same manner as in Example 3;

1.4 Evian natural mineral water: purchased at Carrefour supermarket;

1.5 using the same method as in Example 3 to treat drinking water made from Tibetan glacial mineral water;

1.6 Tibet Glacier Mineral Water: Purchase at Carrefour supermarket;

1.7 ultrapure water: made by the German Sartorius ultrapure water preparation system;

1.8 fiber spectrometer Ava-Spec3648, measuring range 200-1100nm, uncertainty / accuracy 0.5nm;

1.9 oscilloscope MS04104, equipment factory number: C001163, measurement accuracy: 1GHz;

1.10 Photodetector Thorlab DET10A/M, equipment ex-factory number: JGZX-ZXZC-012, measurement accuracy: 200nm-1100nm;

1.11 Test conditions: temperature 21 ° C, humidity: 38%.

2, experimental methods

2.1 broad spectrum absorption rate

(1) Rinse the experimental utensils with about 200 ml of ultrapure water until the physicochemical indexes are stable.

(2) The ordinary drinking water raw water used in Example 3 and the micro-kinetic energy drinking water prepared in Example 3 were placed in an experimental vessel, and the absorption of the spectral light spectrum of the standard light source of the sample of 200 nm to 800 nm was scanned, and the experimental results were recorded.

(3) Rinse the experimental utensils with about 200 ml of ultrapure water until the physicochemical indexes are restored to their original values and stabilized.

(4) Evian natural mineral water and drinking water prepared by treating Evian natural mineral water in the same manner as in Example 3 were placed in an experimental vessel, and step (2) was repeated to record the amount of change in ultraviolet intensity during the recording.

(5) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.

(6) The Tibetan glacial mineral water and the drinking water prepared by treating the Tibetan glacial mineral water by the same method as in Example 3 were placed in an experimental vessel, and the step (2) was repeated to record the amount of change in the ultraviolet intensity during the recording.

(7) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.

2.2 UV absorption rate determination

(1) Rinse the experimental utensils with about 200 ml of ultrapure water until the physicochemical indexes are stable.

(2) The micro-kinetic energy prepared in Example 3 is placed in an ultraviolet intensity detecting system, the ultraviolet light source illuminates the water to be tested, and the photodetector ThorlabDET10A/M is read by an oscilloscope MS04104 to read the ultraviolet intensity of the water not to be tested. The value is read by the value of the ultraviolet intensity of the water to be tested, continuously tested continuously for 72 hours, and the amount of change in ultraviolet intensity during the recording.

(3) Rinse the experimental utensils with about 200ml of ultrapure water until the physical and chemical indicators are restored. The value is stable.

(4) The drinking water prepared by the Evian natural mineral water in the same manner as in Example 3 was placed in the ultraviolet intensity detecting system, and the step (2) was repeated to record the amount of change in the ultraviolet intensity during the recording.

(5) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.

(6) The drinking water made from the Tibetan glacial mineral water in the same manner as in Example 3 is placed in the ultraviolet intensity detecting system, and the step (2) is repeated to record the amount of change in the ultraviolet intensity during the recording.

(7) Rinse the experimental utensils with about 200 ml of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.

3. Experimental results

3.1 broad spectrum absorption rate test

Results are shown in Figure 3-5.

3.2 UV absorption rate determination

Multi-pole micro-kinetic energy drinking water made from ordinary water:

UV absorption rate: 0.9135->0.9037

Multi-pole micro-kinetic energy drinking water made from Evian natural mineral water:

UV absorption rate: 0.9244->0.9188

Multi-pole micro-kinetic energy drinking water made from Tibetan glacial mineral water:

UV absorption rate: 0.9157->0.9079

4. Experimental conclusions and analysis

Through the above experiments, the following conclusions were obtained:

(1) Compared with the raw water, the absorption spectrum absorption peak of the multi-pole micro-kinetic energy drinking water of the present invention is shifted to a short wave by about 30 nm;

(2) Under the 72-hour test, the absorption rate of the multi-pole micro-kinetic energy drinking water of the present invention decreased by 1%.

Example 8 Variation of stimulated fluorescence spectrum of multi-pole micro-kinetic energy drinking water of the present invention

1. Experimental materials and equipment

1.1 The kinetic energy drinking water prepared in Example 3;

1.2 ordinary drinking water raw water used in embodiment 3;

1.3 treating the drinking water made of Evian natural mineral water in the same manner as in Example 3;

1.4 Evian natural mineral water: purchased at Carrefour supermarket;

1.5 using the same method as in Example 3 to treat drinking water made from Tibetan glacial mineral water;

1.6 Tibet Glacier Mineral Water: Purchase at Carrefour supermarket;

1.7 ultrapure water: made by the German Sartorius ultrapure water preparation system;

1.8 fiber spectrometer Ava-Spec3648, measuring range 200-1100nm, uncertainty / accuracy 0.5nm;

1.9 Test environment: temperature 22 ° C, humidity 37%.

2, experimental methods

(1) Rinse the experimental utensils with about 200 mL of ultrapure water until the physicochemical indexes are stable.

(2) The micro-kinetic energy prepared in Example 3 is placed in a sample cell of a fiber optic spectrometer, and an excitation pulse is given to the sample by a 266 nm pulsed ultraviolet laser, and the spectral spectrum of the sample is used to measure the emission spectrum of the sample. Fluorescence emission spectrum of the sample.

(3) Rinse the experimental utensils with about 200 mL of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.

(4) The ordinary drinking water raw water used in Example 3 was placed in the fiber optic spectrometer sample cell, and step 2 was repeated to record the experimental data.

(5) Rinse the experimental utensils with about 200 mL of ultrapure water until the physical and chemical indexes are restored to their original values and stabilized.

(6) The drinking water prepared by treating Evian natural mineral water in the same manner as in Example 3 was placed in the spectrometer sample cell, and steps 2 and 3 were repeated to record the experimental data.

(7) Place the Evian natural mineral water in the fiber optic spectrometer sample cell and repeat steps 2 and 3 to record the experimental data.

(8) The drinking water of the Tibetan glacial mineral water is treated in the sample cell of the optical fiber spectrometer prepared by the same method as in Example 3, and the experimental data is recorded in steps 2 and 3.

(9) Place the Tibetan glacial mineral water in the fiber optic spectrometer sample cell and repeat steps 2 and 3 to record the experimental data.

(10) Three samples of drinking water prepared from the Tibetan glacial mineral water in the same manner as in Example 3 were placed in the sample of the optical fiber spectrometer, and the experimental data were recorded in steps 2 and 3.

3. Experimental results

3.1 Fluorescence steady state transient test

The fluorescence steady-state transient test results of ordinary drinking water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 6. Fluorescence steady state transient of Evian natural mineral water and multi-pole micro-kinetic energy drinking water made thereof The test results are shown in Figure 7. The fluorescence steady-state transient test results of Tibetan glacial mineral water and multi-pole micro-kinetic drinking water made by it are shown in Fig. 8. The glacial mineral water in Tibet was tested for repeatability by taking three samples of multi-polar micro-kinetic energy drinking water made of raw water. The results are shown in Figure 9.

4. Experimental conclusions and analysis

It can be seen from the above experimental results that the fluorescence intensity intensity of the three different samples of the multi-pole micro-kinetic energy drinking water of the present invention is greatly improved in the 300 nm-400 nm spectrum, and is repeated for the same sample. Experiments show that the experiment has good repeatability.

Experiments have shown that the multi-pole micro-kinetic energy drinking water treated by the method of the present invention has more extra-nuclear electrons in a high-energy non-radiative energy level.

Example 9 Multi-polar micro-kinetic energy drinking water anti-fatigue function of the present invention: serum lactic acid in mice The effect of the content and the effect on the weight-bearing swimming time of mice

1. Materials and methods

1.1 sample:

The multi-pole micro-kinetic energy drinking water (kinetic energy water) prepared in Example 3 is a colorless and odorless transparent aqueous liquid, which is stored at room temperature and used within 72 hours after preparation;

Ang Lixi ginseng capsule, produced by Shanghai Jiaotong University Ang Li Co., Ltd., batch number C2102001.

1.2 Experimental animals: ICR mice, purchased from Beijing Huakangkang Biotechnology Co., Ltd., production license number: SCXK (Beijing) 2007-0001, experimental animal quality certificate number: 11401300009361. 40, male, each group 10 Only, a total of 4 groups, order weight 18-20g.

Feeding conditions: Experimental animal facilities continue to maintain barrier environmental standards. The control range of the main environmental indicators: room temperature 20 ~ 26 ° C, the daily temperature difference ≤ 4 ° C. The relative humidity is 40 to 70%. Minimum air exchange times 15 times / hour, light illumination: dark = 12h: 12h. The animals are housed in a polypropylene rat group cage. The cage specifications are: 545×395×200mm ³ , 5 per box, and the feeding space meets the minimum space required for experimental animals in the national standard GB14925-2010 of the People's Republic of China. Provisions. All animals are managed by trained personnel. Replace the litter and cage once a week. Rat-specific feed is added daily for animal consumption, and the animal's diet is kept free during the whole feeding process.

Animal welfare: The animals used in this experiment and related disposals should meet the requirements of animal welfare. Before the experiment, the animal management and use committee (IACUC) of the New Drug Safety Evaluation Research Center of Peking Union Medical College of Chinese Academy of Medical Sciences was approved. The specific considerations are as follows:

(1) Animal panic and pain should be minimized during the experiment.

(2) Effective anesthesia must be performed when performing and dissecting experimental animals. Postoperative During the recovery period, analgesia and targeted care and diet conditioning should be performed according to the actual situation.

(3) When Baoding experimental animals, avoid causing uneasiness, panic, pain and damage to the animals. Baoding appliances should be of reasonable structure, appropriate specifications, sturdy and durable, environmentally friendly and easy to operate. The mandatory restrictions on the animal's body should be reduced to a minimum without affecting the experiment.

(4) Sample collection: The operation of collecting samples for experimental animals in a safe and humane manner (minimizing the tension and discomfort caused to animals).

(5) For sudden death, sick animals and animals with serious toxic reactions, report to the veterinarian and the person in charge of the case in time for timely treatment or euthanasia. In the case of not affecting the judgment of the experimental results, choose "benevolent end point" to avoid prolonging the time the animal suffers. When the experimental animals were sacrificed, euthanasia was carried out in accordance with humanitarian principles. At the scene of the execution, no other animals were present. The body can be properly disposed of after confirming the death of the animal.

(6) Occupational safety: During the experiment, take personal protective measures such as gloves, gowns, masks, eye masks, earplugs, etc.

The test was divided into 4 groups: the blank control group drank normal drinking water daily (normal daily drinking water 0.3mL/10g body weight); the kinetic energy low dose group daily drink kinetic energy (drinking bottle filling kinetic energy, replace 1 per day) The high-dose group of kinetic energy water, except for the daily basic kinetic energy water, was once perfused with kinetic energy once a day at a dose of 0.3 mL/10 g body weight; the Ang Lixi ginseng capsule group was administered once a day at a dose of 0.6 g/ Kg, the dosage volume is 0.3mL/10g body weight (Angstrom ginseng capsules are 4 capsules per person per day, the net content of each capsule is 0.28g, and the human body weight is calculated according to 60kg, which is equivalent to 0.01867g/kg. For human use, 30 times, 0.56 g/kg, the dosage volume is 30 mL/kg, the drug preparation concentration is 0.0186 mg/mL, the reagent preparation concentration is 0.02 g/mL, and the actual dose is 0.6 g/kg). There were no fasting and water prohibition requirements during the experiment. The lactic acid measurement test was continued for 33 days, and the swimming test was carried out 2 days after the administration, and continuous administration was carried out for 35 days.

1.4 Main instruments and reagents: lactic acid meter, Lactate Scout+, Germany EKF; lactic acid tester special test paper, batch number: 3312307, 33113351, 3312307, Germany EKF, timer.

1.5 test method:

Blood lactic acid test: continuous administration for 33 days, blood collection after 30 minutes of the last administration, determination of blood lactate measured value before swimming; then without weight, stop after swimming for 10 minutes in water at 30 ° C, immediately take blood to measure lactic acid; rest after 20 minutes again Blood collection measurement. The blood collection method is blood sampling by tail vein acupuncture, and the blood collection amount is at least 0.5 μL.

Weight-bearing swimming test: The same batch of animals continued to be administered for 2 days for a total of 35 days. After giving the test sample 30 times, the mice with 5% body weight lead skin at the base of the tail were placed in a swimming pool for swimming. The water depth is not less than 30cm, and the water temperature is 25°C±1.0°C. The time from the start of swimming to the time of sinking into the water is recorded, that is, the swimming time of the mouse.

1.6 Test data statistics:

The weight results were processed using SPSS statistical software and compared between the drug-administered group and the control group. According to the following method: the Kolmogorov-Smirnov method is used for the normal test, and the Levene median method is used to test the homogeneity of variance. If P>0.05, the one-way ANOVA method is used, if the normal and the variance are homogeneous. The sex test failed (P ≤ 0.05) and a non-parametric Mann-Whitney test was performed.

1.7 Determination of results:

The lactic acid measurement data is the measurement data, and the variance analysis is used. However, the homogeneity test of variance is first performed according to the procedure of variance analysis. The variance is calculated and the F value is calculated. The F value is <0.05. Conclusion: There is no significant difference between the mean of each group; The value is ≥0.05, P≤0.05, and the two-two comparison method between the multiple experimental groups and one control group is used for statistical calculation; the non-normal or variance data is subjected to appropriate variable conversion, and the normal or variance is to be satisfied. After the requirements are met, the converted data is used for statistics; if the variables are still not up to the normal or variance, the rank sum test is used for statistics.

If the lactic acid value of the test sample group is significantly smaller than that of the control group, and the difference is significant, the experimental result can be judged to be positive.

The swimming time is the measurement data, and the variance analysis is used. However, the homogeneity test of variance is first performed according to the procedure of variance analysis. The variance is calculated and the F value is calculated. The F value is <0.05. Conclusion: There is no significant difference between the mean of each group; F value ≥0.05, P≤0.05, using two-two comparison methods of the mean between multiple experimental groups and one control group for statistical analysis; appropriate variable conversion for non-normal or variance data, to be satisfied with normal or variance After the request, the converted data is used for statistics; if the variable has not yet reached the normal or variance, the rank sum test is used for statistics.

If the weight-bearing swimming time of the test sample group is significantly longer than that of the control group, and the difference is significant, the experimental result can be judged to be positive. Or analyze the results according to the actual conditions of the test object.

2. Results

The results are shown in Table 2, Table 3, and Figure 10.

Table 2 Effect of multi-polar micro-kinetic energy drinking water of the present invention on serum lactic acid content of mice (mmol/L,

n=10)

It can be seen from Table 2 that there is no statistical difference in the measured value of blood lactate before and after swimming, immediately after swimming, 20 minutes after swimming, low dose of kinetic energy, high dose of kinetic energy and blank control group, the absolute value is more consistent; the positive drug American ginseng There was no statistical difference between the mice in the group before swimming, immediately after swimming for 10 minutes and 10 minutes after the swimming, and the absolute values were consistent. It is suggested that in this experiment, kinetic energy and positive drug American ginseng have no significant effect on blood lactate value before and after swimming in mice.

Table 3. Effect of kinetic energy on the weight-bearing swimming time of mice (min,

n=10)

It can be seen from Table 3 and Figure 10. In the weight-bearing swimming experiment, the average swimming time of the blank control group was 9.54±4.64min, the average swimming time of the low-dose group of kinetic energy was 11.62±5.40min, and the average swimming time of the high-activity group of kinetic energy was 13.08. ±6.94min, the average swimming time of the positive drug American ginseng group was 14.21±6.28min. Although no statistical difference was found, the absolute value of swimming time in mice with low kinetic energy and high dose group increased compared with the blank control group, and showed a certain dose-response relationship. Although there was no statistical difference in the positive drug American ginseng group, However, the absolute value of swimming time in mice also increased significantly. It is suggested that kinetic energy has the effect of prolonging the swimming time of mice.

3. Summary

3.1 blood lactate determination experiment

Under the experimental conditions, the multi-polar micro-kinetic energy drinking water and the positive drug American ginseng of the present invention have no significant effect on the blood lactate value before and after swimming in mice.

3.2 mouse weight swimming test swimming time

Under the experimental conditions, the results suggest that the multi-pole micro-kinetic energy drinking water of the present invention has the effect of prolonging the swimming time of the weight-bearing mouse.

Claims (10)

1. Use of multi-pole micro-kinetic energy drinking water in the preparation of beverages, health care products or medicines for use in anti-fatigue.
2. The use according to claim 1, wherein the multi-pole micro-kinetic energy drinking water is prepared by non-contact treatment of drinking water raw water with low, medium and high electromagnetic waves of different frequencies, wherein the frequency of the low frequency electromagnetic wave The range is 30-100 kHz, the frequency range of the intermediate frequency electromagnetic wave is 550-720 kHz, and the frequency range of the high frequency electromagnetic wave is 300-725 MHz.
3. The use according to claim 1 or 2, characterized in that the raw water of drinking water is ordinary tap water or various kinds of mineral water or pure water, such as Evian natural mineral water, Tibet glacier mineral water, Nongfu Spring, Wahaha, etc. .
4. The use according to any one of claims 1 to 3, characterized in that the frequency of the low frequency electromagnetic wave is in the range of 45-75 kHz;

   Preferably, the frequency of the intermediate frequency electromagnetic wave ranges from 600 to 720 kHz;

   Preferably, the high frequency electromagnetic wave has a frequency in the range of 300-425 MHz.
5. The use according to any one of claims 1 to 4, wherein the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave are sine waves, square waves, sharp waves, sawtooth waves or trapezoidal waves;

   Preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform are the same or different;

   More preferably, the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave waveform are preferably the same.
6. The use according to any one of claims 1 to 5, characterized in that the low frequency electromagnetic wave and the intermediate frequency electromagnetic wave propagate in the same direction.
7. The use according to any one of claims 1 to 6, wherein the high-frequency electromagnetic wave is a triangular wave and is perpendicular to a propagation direction of the low-frequency electromagnetic wave and the intermediate-frequency electromagnetic wave.
8. The use according to any one of claims 1 to 7, wherein the low-frequency electromagnetic wave and the high-frequency electromagnetic wave are simultaneously processed by non-contact treatment of drinking water raw water by using three different frequencies of low, medium and high frequencies. And then using intermediate frequency electromagnetic waves and high frequency electromagnetic waves for simultaneous processing;

   Preferably, the time for simultaneous treatment using low frequency electromagnetic waves and high frequency electromagnetic waves is 10-30 minutes, preferably 10-20 minutes;

   More preferably, the time for simultaneous treatment using the intermediate frequency electromagnetic wave and the high frequency electromagnetic wave is 10 to 30 minutes, preferably 10 to 20 minutes.
9. Use according to any one of claims 1 to 8, characterized in that it is The ultraviolet absorption peak of the multi-pole micro-kinetic energy drinking water is shifted by 25 nm to 40 nm in the short-wave direction, preferably 28 nm to 30 nm in the short-wave direction, and most preferably 30 nm or 28 nm in the short-wave direction.
10. The use according to any one of claims 1 to 9, wherein the beverage is a functional beverage and can be used for fatigue resistance;

    Preferably, the health supplement or drug can be used to combat fatigue.

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