WO2016146029A1 - A process for producing a product with high content of natural vitamin c - Google Patents

A process for producing a product with high content of natural vitamin c Download PDF

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Publication number
WO2016146029A1
WO2016146029A1 PCT/CN2016/076136 CN2016076136W WO2016146029A1 WO 2016146029 A1 WO2016146029 A1 WO 2016146029A1 CN 2016076136 W CN2016076136 W CN 2016076136W WO 2016146029 A1 WO2016146029 A1 WO 2016146029A1
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content
juice
rosa roxburghii
vitamin
fermented
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PCT/CN2016/076136
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French (fr)
Inventor
Qing He
Hai Ning ZHAO
Xiaojia HU
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Dsm Ip Assets B.V.
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Publication of WO2016146029A1 publication Critical patent/WO2016146029A1/en

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/385—Concentrates of non-alcoholic beverages
    • A23L2/39—Dry compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A23L2/08—Concentrating or drying of juices
    • A23L2/10—Concentrating or drying of juices by heating or contact with dry gases
    • A23L2/102—Spray-drying
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A23L2/08—Concentrating or drying of juices
    • A23L2/12—Concentrating or drying of juices by freezing
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/38—Other non-alcoholic beverages
    • A23L2/382—Other non-alcoholic beverages fermented
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins

Definitions

  • the present invention is related to a process for producing a product with high content of natural vitamin C, and also the product with high content of natural vitamin C produced by the process.
  • Vitamin C also named ascorbic acid, is one of water soluble vitamins. Vitamin C is an essential element for life. It can prevent cold and scurvy, improve the immunity and has benefit in cosmetology and cancer. It is widely applied in health care, beverages and beauty.
  • Vegetable and fruits are the primary resources to obtain vitamin C for human being. However, the daily intake of vegetable and fruits can’ t meet the requirement of vitamin C for human, and generally, additional vitamin C should be supplemented. At present, most of vitamin C in the market is synthetic, and natural vitamin C, which is obtained from natural plant for instance vegetable and fruits, is becoming more and more popular.
  • the available natural vitamin C is mainly obtained from extraction of Acerola Cherry.
  • Acerola Cherry also named Malpighia Emarginata, is originated from Tropical America but now is mainly planted in Brazil.
  • Acerola Cherry is found to be rich in vitamin C with content of up to 40000 mg/kg in the year of 1946, so it is called as “king of natural vitamin C” .
  • the Acerola Cherry exported from Brazil is quite expensive and the content of vitamin C produced according to the prior art can only reach around 10%, so it cannot meet the requirements of markets, especially the needs from the market of China.
  • Rosa Roxbunghii is a wild fruit originated from China and mainly from Yuannan, Guizhou, Guangxi and other provinces in China. Rosa Roxbunghii fresh fruit is rich in various of nutritive material including carbohydrates, proteins, amino acids, vitamins, tannins, flavones, microelements, superoxide dismutase (SOD) , catalase, and various unsaturated fatty acids and other active substances required by the human body such as linoleic acid and linolenic acid and the like. Among, the content of vitamin C is extremely high, up to 35411 mg/kg. (see Chinese patent publication CN101926496B) At present, Rosa Roxbunghii is often made to fruit juice. However, the Rosa Roxbunghii juice cannot be applied widely in industry as it cannot be kept for long time and further has acidity and bitter flavor.
  • Chinese patent publication CN101073433B discloses a process for processing Rosa Roxbunghii juice to solid products, comprising adding gelatin and chitosan into Rosa Roxbunghii juice, and adding auxiliary material for granulating.
  • the Chinese patent publication CN101156670B further discloses a process for producing Rosa Roxbunghii powder, comprising treating Rosa Roxbunghii juice by pectinase, adding carriers for drying after ultrafiltration and concentration to give Rosa Roxbunghii powder.
  • the process is not easy to operate and the obtained solid preparation has low content of vitamin C and cannot meet the needs of market.
  • the present invention provides a novel process for producing a product with high content of natural vitamin C from Rosa Roxbunghii as raw material, comprising::
  • the invention also provides a product with high content of vitamin C produced by the above process.
  • the process according to the invention is easy to operate under mild conditions, and the product with high content of natural vitamin C obtained from the process has mellow taste but no astringent taste, so it is suitable for adding into different kinds of food. Further, it contains natural vitamin C of more than 15%, even more than 20%, so it may be used in industry widely.
  • the “high content” means the natural vitamin C it refers to has the content of more than 15%, preferably more than 17%, more preferably more than 20% or 25%, and the most preferably more than 30%.
  • the “natural vitamin C” refers to the vitamin C extracted from natural plant or fruits, so as to distinguish it from the vitamin C obtained by artificial fermentation or synthesis.
  • the “product” refers to goods which can be provided to market, be used and consumed by people and can meet some requirement from people.
  • the “product” is the solid or liquid product, for example but not limited to powder, oral liquid, particle dose, tablet, capsule and pill, etc., preferably, the “product” is powder.
  • the invention provides a process of producing a product with high content of natural vitamin C, comprising:
  • the Rosa Roxburghii juice in the step a) may be obtained commercially, for example from Guizhou Shanzhenbao Green Technology Development Co., Ltd., Guizhou South China University of Technology Bio-Engineering Co., Ltd., etc..
  • the Rosa Roxburghii juice may also be obtained by direct squeeze of Rosa Roxburghii fruits.
  • the squeeze process is well known in the art, for example, by washing freshly picked Rosa Roxburghii fruits, and squeezing them by juicer after proper crush to obtain juice.
  • the Rosa Roxburghii juice in the step a) may also be obtained from Rosa Roxburghii pomace produced from the squeeze of Rosa Roxburghii fruits, for example by extraction in a solvent such as water to obtain Rosa Roxburghii juice.
  • a solvent helpful to destroy cell walls may be added in the extraction, such as pectinase which is helpful to release natural vitamin C from pomace, and/or vitamin C protective agents such as citric acid and the like which are helpful for protecting natural vitamin C from being destroyed in the extraction.
  • Rosa Roxburghii fruits are squeezed directly and the Rosa Roxburghii pomace obtained from the squeeze is extracted to produce Rosa Roxburghii juice in the step a) of the process according to the invention.
  • the content of solid matter is from 1% to 10%, and the content of vitamin C is from 0.1% to 2.5%.
  • the juice is also rich in SOD, flavone, inositol and polyphenol, wherein the content of SOD is usually from 600 U/g to 6000 U/g, the content of flavone is from 0.1% to 1.5%, the content of inositol is from 0.02 to 0.3% and the content of polyphenol is from 0.1% to 2.5%.
  • the content of solid matter and vitamin C in the Rosa Roxburghii juice obtained in the step a) is not important, i.e. the Rosa Roxburghii juice with any content of solid matter and vitamin C may be used in the step b) of the invention.
  • the Rosa Roxburghii juice used in the step b) of the process according to the inventions has preferably the content of solid matter of from 5% to 30% and the content of vitamin C of from 0.5% to 6%.
  • the Rosa Roxburghii juice obtained in the step a) is preferably concentrated before going to the step b) , such as 2 times to 10 times, preferably 3 times to 6 times such as 3, 4 or 5 times.
  • the Rosa Roxburghii juice obtained in the step a) or the condensed juice thereof is fermented by yeast.
  • the yeast may be any yeast applicable for food fermentation, for example but not limited to bread yeast (Saccharomyces cerevisiae) , brewer yeast (Saccharomyces bayanus, Saccharomyces cerevisiae or Saccharomyces uvarum) , yeast with special functions, Hansenula spp and Rhizopus oryzae etc..
  • the yeast may be easily obtained commercially. It may be dried yeast or fresh yeast, and may be used directly or after activation in water. Based on the weight of solid matter in the fermented Rosa Roxburghii juice or the condensed juice thereof, the yeast is added in an amount of from 4% to 10%, preferably 6%.
  • pH modifier may be added into the Rosa Roxburghii juice obtained in the step a) or the condensed juice thereof to adjust the pH value to the required level for fermentation before the fermentation.
  • the example of the pH modifier includes but is not limited to alkali such as potassium hydroxide, sodium hydroxide, calcium hydroxide, potassium carbonate, sodium carbonate, ammonia water, potassium bicarbonate, sodium bicarbonate, sodium sesquicarbonate, ammonium bicarbonate, diammonium hydrogen phosphate, dipotassium phosphate, disodium hydrogen phosphate, tripotassium phosphate, trisodium phosphate, and sodium acetate.
  • the pH modifier is not needed when the pH value of the Rosa Roxburghii juice obtained in the step a) or the condensed juice thereof falls into the range of the preferable pH values.
  • the process of the invention does not need any pH modifier.
  • the fermentation is carried out at the temperature of from 10°C to 50°C, preferably from 20°C to 40°C, more preferably 30°C.
  • the fermentation may last from 2 hours to 20 hours, preferably from 5 hours to 16 hours, more preferably from 6 hours to 10 hours.
  • the fermentation is carried out under stirring.
  • the fermentation of the step b) removes the undesired non-vitamin C components contained in the Rosa Roxburghii juice, such as sugar, carbohydrate, sterol, triterpene, protein, amino acid and fatty acid, etc., while retaining flavone, polyphenol, inositol and SOD components which are good for human health.
  • the yeast may be removed by the process known in the art, such as filtration, to obtain fermented Rosa Roxburghii juice.
  • the fermented Rosa Roxburghii juice obtained in the step b) has solid matter with the content of from 0.5% to 25% and vitamin C with the content of from 0.1% to 6%.
  • the content of vitamin C in the fermented Rosa Roxburghii juice is increased by almost 30%, based on the solid matter.
  • there is almost no loss of the content of flavone, polyphenol, inositol and SOD which are good for human health wherein flavone has the content of from 0.1% to 2%, polyphenol has the content of from 0.1% to 5%, inositol has the content of from 0.02% to 0.5% and SOD has the content of from 600 U/g to 15000 U/g.
  • the fermented Rosa Roxburghii juice obtained in the step b) may be concentrated according to the step c) to provide the concentrated fermentation liquid.
  • the concentration may be carried out by any method known in the art, such as vacuum concentration.
  • the concentrated fermentation liquid preferably contains solid matter with the content of from 1% to 25% and vitamin C with the content of from 0.2% to 6%.
  • the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) may be dried according to the step d) to obtain a product with high content of natural vitamin C.
  • the drying may be carried out by a process known in the art, such as lyophilization.
  • the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) is sprayed drying to obtain solid powders with high content of natural vitamin C.
  • an auxiliary material may be added into the solution to be sprayed drying.
  • the auxiliary material useful for the spray drying of the invention includes but not is limited to pregelatinized starch, soluble potato starch, soluble corn starch, dextrin, maltodextrin, cyclodextrin and carboxymethylcellulose.
  • the auxiliary material is maltodextrin, such as maltodextrin DE12.
  • the auxiliary material may be added into the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) in any amount suitable for the invention.
  • the auxiliary material is added in an amount of from 20% to 50%, preferably from 30% to 40%, based on the weight of solid matter in the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) .
  • the spray drying of the invention is carried out in a spray dryer.
  • the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) forms solid powders after the spray drying. Any person skilled in the art would be easy to use the solid powders to prepare different types of preparations such as oral liquid, granula, tablets, capsules and pills, etc. according to the actual requirements.
  • the product with high content of natural vitamin C obtained in the step d) contains natural vitamin C with the content of from 15% to 30%, and further contains flavone, polyphenol, inositol and SOD which are good for human health, wherein flavone has the content of from 5% to 15%, polyphenol has the content of from 15% to 35%, inositol has the content of from 1% to 3% and SOD has the content of from 50000 U/g to 150000 U/g.
  • the process according to the invention uses china-specific Rosa Roxburghii fruit as raw material to produce natural vitamin C products, has mild condition and is easy to operate. Moreover, the product obtained by the process according to the invention has a high content of natural vitamin C while retaining flavone and polyphenol components which are good for human health in Rosa roxburghii, and has significant improvement of antioxidant ability compared with the existing products.
  • the invention also provides a product with high content of natural vitamin C, which is produced according to the above process according to the invention.
  • the product with high content of natural vitamin C has the content of natural vitamin C of more than 15%, even more than 17% or 20%, while retaining flavone and polyphenol components which are good for human health in Rosa Roxburghii, and has the significant improvement of antioxidant ability compared with the existing products.
  • the content of solid matter is measured as below in accordance with the method for extract as described in the annex X A of the Pharmacopoeia of the People's Republic of China (2010) : precisely weighting a sample; placing the sample in a dry evaporation pan; after drying by distillation in water bash, drying the sample for 3 hours at 105°C; cooling down for 30mins in a dryer; and weighting the dry product quickly and precisely.
  • the content of vitamin C is measured by HPLC.
  • HPLC System is Agilent 1200 series (Agilent technologies (China) Co., Ltd., China) , chromatographic column: YMC hydrosphere C18, 5 ⁇ m, 150 ⁇ 4.6 mm; Mobile phase A: sodium dihydrogen phosphate-phosphoric acid solution (pH 2.0 ⁇ 0.1) , mobile phase B: methyl alcohol; chromatographic condition: 0-5.0 min, B: 0%, 0.8 ml/min; 5.0-5.1 min, B: 70%, 1.2 ml/min; 5.1-9.0 min, B: 70%, 1.2 ml/min; 9.0-9.1 min, B: 0%, 0.8 ml/min; 9.1-20.0 min, B: 0%, 0.8 ml/min; column temperature: 20 °C; detection wavelength: 243 nm; injection volume: 10 ⁇ L.
  • the content of flavone is measured in accordance with the UV-VIS spectrophotometry as described in the annex V A of Pharmacopoeia of the People's Republic of China (2010) , and the content of total polyphenol is measured in accordance with the method for determining tannin content recorded in the annex X B of Pharmacopoeia of the People's Republic of China (2010) , and the content of SOD is measured in accordance with the method for determining SOD activity as described in health food section of GBT5009.171-2003.
  • the content of inositol is measured in accordance with the method for determining inositol as described in the health food section of GB/T 5009.196-2003.
  • Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . 2000.0 Kg of fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, 1280.0 Kg of Rosa Roxburghii juice was obtained. By measurement, the content of solid matter was 8.83%, the content of vitamin C was 1.36%, the content of SOD was 4500 U/g, the content of flavone was 0.90%, the content of inositol was 0.20% and the content of polyphenol was 2.02% in the Rosa Roxburghii juice.
  • Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, filter cake was obtained. 50.0 g of the filter cake was dipped in an equivalent amount of water (50.0 g) at 30°C. After being immersed for 30 mins, filtration under pressure was carried out to obtain 35.0 g of Rosa Roxburghii juice.
  • the content of solid matter was 2.62%
  • the content of vitamin C was 0.42%
  • the content of SOD was 1360 U/g
  • the content of flavone was 0.27%
  • the content of inositol was 0.06%
  • the content of polyphenol was 0.60% in the Rosa Roxburghii juice.
  • Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, filter cake was obtained. 50.0 g of the filter cake was dipped in 5 eq. (250.0 g) 1.0% pectinase aqueous solution at 30°C. After being immersed for 30 mins, filtration under pressure was carried out to obtain 216.0 g of Rosa Roxburghii juice.
  • the content of solid matter was 1.32%
  • the content of vitamin C was 0.18%
  • the content of SOD was 686 U/g
  • the content of flavone was 0.13 %
  • the content of inositol was 0.03%
  • the content of polyphenol was 0.30% in the Rosa Roxburghii juice.
  • Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, filter cake was obtained. 100.0 Kg of the filter cake was dipped in 1.2 eq (120.0 Kg) 0.5% citric acid aqueous solution at 50°C. After being immersed for 180 mins, filtration under pressure was carried out to obtain 140.0 Kg of Rosa Roxburghii juice.
  • the content of solid matter was 7.01 %
  • the content of vitamin C was 0.68%
  • the content of SOD was 3640 U/g
  • the content of flavone was 0.72%
  • the content of inositol was 0.16%
  • the content of polyphenol was 1.60% in the Rosa Roxburghii juice.
  • Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . 6000.0 Kg of fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, juice and 2440.0 Kg of filter cake was obtained. The filter cake was dipped in 1.2 eq (2928.0 Kg) 0.5% citric acid aqueous solution at 40°C. After being immersed for 30 mins, filtration under pressure was carried out. Totally 5208.0 Kg of Rosa Roxburghii juice was obtained.
  • the content of solid matter was 5.78%
  • the content of vitamin C was 0.99%
  • the content of SOD was 3000 U/g
  • the content of flavone was 0.59%
  • the content of inositol was 0.13%
  • the content of polyphenol was 1.32% in the Rosa Roxburghii juice.
  • Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed, After filtration under pressure, 1000.0 mL of Rosa Roxburghii juice was obtained. The Rosa Roxburghii juice was evaporated to obtain 200.0 mL of concentrated Rosa Roxburghii juice at 55°C. After measurement, the content of solid matter was 22.41%, the content of vitamin C was 4.02%, the content of SOD was 11645 U/g, the content of flavone was 2.29%, the content of inositol was 0.51% and the content of polyphenol was 5.12% in the juice.
  • the content of solid matter was 3.29%
  • the content of vitamin C was 1.03%
  • the content of SOD was 1711 U/g
  • the content of flavone was 0.34%
  • the content of inositol was 0.07%
  • the content of polyphenol was 0.75% in the fermented Rosa Roxburghii juice.
  • Example 8 Fermentation of Rosa Roxburghii juice by using fresh yeast
  • the content of solid matter was 3.23%
  • the content of vitamin C was 0.99%
  • the content of SOD was 1802 U/g
  • the content of flavone was 0.33%
  • the content of inositol was 0.07%
  • the content of polyphenol was 0.72% in the fermented Rosa Roxburghii juice.
  • the content of solid matter was 14.03 %
  • the content of vitamin C was 4.10 %
  • the content of SOD was 12995 U/g
  • the content of flavone was 1.95 %
  • the content of inositol was 0.32%
  • the content of polyphenol was 4.13% in the fermented Rosa Roxburghii juice.
  • the content of solid matter was 16.29%
  • the content of vitamin C was 3.83%
  • the content of SOD was 11649 U/g
  • the content of flavone was 1.75%
  • the content of inositol was 0.37%
  • the content of polyphenol was 3.70% in the fermented Rosa Roxburghii juice.
  • Example 11 Spray drying of the fermented Rosa Roxburghii juice
  • the yield was 78%.
  • the content of vitamin C was 21.46 %
  • the content of SOD was 115753 U/g
  • the content of flavone was 9.95 %
  • the content of inositol was 2.28 %
  • the content of polyphenol was 22.70 % in the obtained natural vitamin C powder.
  • Example 12 Spray drying of the fermented Rosa Roxburghii juice
  • the content of vitamin C was 19.04 %
  • the content of SOD was 95640 U/g
  • the content of flavone was 7.62 %
  • the content of inositol was 1.94 %
  • the content of polyphenol was 20.53% in the obtained natural vitamin C powder.
  • Example 13 Spray drying of the fermented Rosa Roxburghii juice
  • the content of vitamin C was 20.13 %
  • the content of SOD was 72993 U/g
  • the content of flavone was 10.38 %
  • the content of inositol was 2.01 %
  • the content of polyphenol was 23.69% in the obtained natural vitamin C powder.
  • Example 14 Vacuum freeze drying of the fermented Rosa Roxburghii juice
  • the antioxidant capacity of the Rosa Roxburghii powder obtained according to the Example 13 (the content of vitamin C was 20.13%) , commercially available acerola cherry powder (the content of vitamin C was 16.72%) and synthetic vitamin C (the content of vitamin C was adjusted to 22.90% by using maltodextrin DE12) was assessed by the hydrophilic antioxidant capacity -antiperoxyl radicals method.
  • the capacity resisting to external ROO- was determined with AAPH as resource of producing ROO-, fluorescein as fluorescence probe and vitamin E water soluble analogue Trolox as standard contrast.

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Abstract

A process for producing a product with high content of natural vitamin C, comprising: a) Obtaining Rosa Roxburghii juice; b) Fermenting the Rosa Roxburghii juice with yeast to obtain fermented Rosa Roxburghii juice; c) Optionally, concentrating the fermented Rosa Roxburghii juice to obtain concentrated fermented juice; and d) Drying the fermented Rosa Roxburghii juice or the concentrated fermented juice to provide the product with high content of natural vitamin C. The product with high content of natural vitamin C obtained from the process contains natural vitamin C of more than 15％, even more than 20％.

Description

A PROCESS FOR PRODUCING A PRODUCT WITH HIGH CONTENT OF NATURAL VITAMIN C
Technical Filed
The present invention is related to a process for producing a product with high content of natural vitamin C, and also the product with high content of natural vitamin C produced by the process.
Background of Invention
Vitamin C, also named ascorbic acid, is one of water soluble vitamins. Vitamin C is an essential element for life. It can prevent cold and scurvy, improve the immunity and has benefit in cosmetology and cancer. It is widely applied in health care, beverages and beauty.
Vegetable and fruits are the primary resources to obtain vitamin C for human being. However, the daily intake of vegetable and fruits can’ t meet the requirement of vitamin C for human, and generally, additional vitamin C should be supplemented. At present, most of vitamin C in the market is synthetic, and natural vitamin C, which is obtained from natural plant for instance vegetable and fruits, is becoming more and more popular.
The available natural vitamin C is mainly obtained from extraction of Acerola Cherry. Acerola Cherry, also named Malpighia Emarginata, is originated from Tropical America but now is mainly planted in Brazil. Acerola Cherry is found to be rich in vitamin C with content of up to 40000 mg/kg in the year of 1946, so it is called as “king of natural vitamin C” . However, the Acerola Cherry exported from Brazil is quite expensive and the content of vitamin C produced according to the prior art can only reach around 10％, so it cannot meet the requirements of markets, especially the needs from the market of China.
Rosa Roxbunghii is a wild fruit originated from China and mainly from Yuannan, Guizhou, Guangxi and other provinces in China. Rosa Roxbunghii fresh fruit is rich in various of nutritive material including carbohydrates, proteins, amino acids, vitamins, tannins, flavones, microelements, superoxide dismutase (SOD) , catalase, and various unsaturated fatty acids and other active substances required by the human body such as linoleic acid and linolenic acid and the like. Among, the content of vitamin C is extremely high, up to 35411 mg/kg. (see Chinese patent publication CN101926496B) At present, Rosa Roxbunghii is often made to fruit juice. However, the Rosa Roxbunghii juice cannot be applied widely in industry as it cannot be kept for long time and further has acidity and bitter flavor.
Chinese patent publication CN101073433B discloses a process for processing Rosa Roxbunghii juice to solid products, comprising adding gelatin and chitosan into Rosa Roxbunghii juice, and adding auxiliary material for granulating. The Chinese patent publication CN101156670B further discloses a process for producing Rosa Roxbunghii powder, comprising treating Rosa Roxbunghii juice by pectinase, adding carriers for drying after ultrafiltration and concentration to give Rosa Roxbunghii powder. However, the process is not easy to operate and the obtained solid preparation has low content of vitamin C and cannot meet the needs of market.
Therefore, a novel process, which can produce a product with high content of natural vitamin C from Rosa Roxbunghii as raw material and is easy to operate and suitable for applying in industry, is in demand.
Summary of the Invention
The present invention provides a novel process for producing a product with high content of natural vitamin C from Rosa Roxbunghii as raw material, comprising:：
a) Obtaining Rosa Roxbunghii juice；
b) Fermenting the Rosa Roxbunghii juice by yeast to obtain the fermented Rosa Roxbunghii juice；
c) Optionally, concentrating the fermented Rosa Roxbunghii juice to obtain the concentrated fermented juice； and
d) Drying the fermented Rosa Roxbunghii juice or the concentrated fermented juice to give a product with high content of natural vitamin C.
The invention also provides a product with high content of vitamin C produced by the above process.
The process according to the invention is easy to operate under mild conditions, and the product with high content of natural vitamin C obtained from the process has mellow taste but no astringent taste, so it is suitable for adding into different kinds of food. Further, it contains natural vitamin C of more than 15％, even more than 20％, so it may be used in industry widely.
Detailed Description of the Invention
In the invention, all the content are by weight percent, except for the content of SOD.
In the invention, the “high content” means the natural vitamin C it refers to has the content of more than 15％, preferably more than 17％, more preferably more than 20％ or 25％, and the most preferably more than 30％.
In the invention, the “natural vitamin C” refers to the vitamin C extracted from natural plant or fruits, so as to distinguish it from the vitamin C obtained by artificial fermentation or synthesis.
In the invention, the “product” refers to goods which can be provided to market, be used and consumed by people and can meet some requirement from people. In the present invention, the “product” is the solid or liquid product, for example but not limited to powder, oral liquid, particle dose, tablet, capsule and pill, etc., preferably, the “product” is powder.
The invention provides a process of producing a product with high content of natural vitamin C, comprising:
a) Obtaining Rosa Roxburghii juice；
b) Fermenting the Rosa Roxburghii juice with yeast to obtain fermented juice；
c) Optionally, concentrating the fermented juice to obtain concentrated fermented juice； and
d) Drying the fermented juice or the concentrated fermented juice to provide the product with high content of natural vitamin C.
In the process according to the invention, the Rosa Roxburghii juice in the step a) may be obtained commercially, for example from Guizhou Shanzhenbao Green Technology Development Co., Ltd., Guizhou South China University of Technology Bio-Engineering Co., Ltd., etc.. In addition, the Rosa Roxburghii juice may also be obtained by direct squeeze of Rosa  Roxburghii fruits. The squeeze process is well known in the art, for example, by washing freshly picked Rosa Roxburghii fruits, and squeezing them by juicer after proper crush to obtain juice.
In the process according to the invention, the Rosa Roxburghii juice in the step a) may also be obtained from Rosa Roxburghii pomace produced from the squeeze of Rosa Roxburghii fruits, for example by extraction in a solvent such as water to obtain Rosa Roxburghii juice. It is understandable by a person skilled in the art that a solvent helpful to destroy cell walls may be added in the extraction, such as pectinase which is helpful to release natural vitamin C from pomace, and/or vitamin C protective agents such as citric acid and the like which are helpful for protecting natural vitamin C from being destroyed in the extraction.
In an embodiment, Rosa Roxburghii fruits are squeezed directly and the Rosa Roxburghii pomace obtained from the squeeze is extracted to produce Rosa Roxburghii juice in the step a) of the process according to the invention.
Generally, in the Rosa Roxburghii juice obtained in the step a) , the content of solid matter is from 1％ to 10％, and the content of vitamin C is from 0.1％ to 2.5％. In addition, the juice is also rich in SOD, flavone, inositol and polyphenol, wherein the content of SOD is usually from 600 U/g to 6000 U/g, the content of flavone is from 0.1％ to 1.5％, the content of inositol is from 0.02 to 0.3％ and the content of polyphenol is from 0.1％ to 2.5％.
In the process according to the invention, the content of solid matter and vitamin C in the Rosa Roxburghii juice obtained in the step a) is not important, i.e. the Rosa Roxburghii juice with any content of solid matter and vitamin C may be used in the step b) of the invention. However, the Rosa Roxburghii juice used in the step b) of the process according to the inventions has preferably the content of solid matter of from 5％ to 30％ and the content of vitamin C of from 0.5％ to 6％. Accordingly, the Rosa Roxburghii juice obtained in the step a) is preferably concentrated before going to the step b) , such as 2 times to 10 times, preferably 3 times to 6 times such as 3, 4 or 5 times.
In the step b) , the Rosa Roxburghii juice obtained in the step a) or the condensed juice thereof is fermented by yeast. The yeast may be any yeast applicable for food fermentation, for example but not limited to bread yeast (Saccharomyces cerevisiae) , brewer yeast (Saccharomyces bayanus, Saccharomyces cerevisiae or Saccharomyces uvarum) , yeast with special functions, Hansenula spp and Rhizopus oryzae etc.. The yeast may be easily obtained commercially. It may be dried yeast or fresh yeast, and may be used directly or after activation in water. Based on the weight of solid matter in the fermented Rosa Roxburghii juice or the condensed juice thereof, the yeast is added in an amount of from 4％ to 10％, preferably 6％.
It is known in the art that yeast may be fermented preferably at pH 3.0-6.0. Therefore, if needed, pH modifier may be added into the Rosa Roxburghii juice obtained in the step a) or the condensed juice thereof to adjust the pH value to the required level for fermentation before the fermentation. The example of the pH modifier includes but is not limited to alkali such as potassium hydroxide, sodium hydroxide, calcium hydroxide, potassium carbonate, sodium carbonate, ammonia water, potassium bicarbonate, sodium bicarbonate, sodium sesquicarbonate, ammonium bicarbonate, diammonium hydrogen phosphate, dipotassium phosphate, disodium hydrogen phosphate, tripotassium phosphate, trisodium phosphate, and sodium acetate. However, it is understandable for any person skilled in the art that the pH modifier is not needed when the pH value of the Rosa Roxburghii juice obtained in the step a) or the condensed juice thereof falls into the range of the preferable pH values. Preferably, the process of the invention does not need any pH modifier.
In the step b) , the fermentation is carried out at the temperature of from 10℃ to 50℃, preferably from 20℃ to 40℃, more preferably 30℃. The fermentation may last from 2 hours to 20 hours, preferably from 5 hours to 16 hours, more preferably from 6 hours to 10 hours. Preferably, the fermentation is carried out under stirring.
In the process according to the invention, the fermentation of the step b) removes the undesired non-vitamin C components contained in the Rosa Roxburghii juice, such as sugar, carbohydrate, sterol, triterpene, protein, amino acid and fatty acid, etc., while retaining flavone, polyphenol, inositol and SOD components which are good for human health. After the fermentation finishes, the yeast may be removed by the process known in the art, such as filtration, to obtain fermented Rosa Roxburghii juice.
Generally, in the process according to the invention, the fermented Rosa Roxburghii juice obtained in the step b) has solid matter with the content of from 0.5％ to 25％ and vitamin C with the content of from 0.1％ to 6％. The content of vitamin C in the fermented Rosa Roxburghii juice is increased by almost 30％, based on the solid matter. In addition, there is almost no loss of the content of flavone, polyphenol, inositol and SOD which are good for human health, wherein flavone has the content of from 0.1％ to 2％, polyphenol has the content of from 0.1％ to 5％, inositol has the content of from 0.02％ to 0.5％ and SOD has the content of from 600 U/g to 15000 U/g.
Optionally, in order to obtain a product with high content of natural vitamin C, the fermented Rosa Roxburghii juice obtained in the step b) may be concentrated according to the step c) to provide the concentrated fermentation liquid. The concentration may be carried out by any method known in the art, such as vacuum concentration. The concentrated fermentation liquid preferably contains solid matter with the content of from 1％ to 25％ and vitamin C with the content of from 0.2％ to 6％.
The fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) may be dried according to the step d) to obtain a product with high content of natural vitamin C.
The drying may be carried out by a process known in the art, such as lyophilization. Preferably, in the process according to the invention, the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) is sprayed drying to obtain solid powders with high content of natural vitamin C.
As known in the art, in order to make the solid powder obtained from spray drying to have better granularity and liquidity, an auxiliary material may be added into the solution to be sprayed drying. The auxiliary material useful for the spray drying of the invention includes but not is limited to pregelatinized starch, soluble potato starch, soluble corn starch, dextrin, maltodextrin, cyclodextrin and carboxymethylcellulose. Preferably, the auxiliary material is maltodextrin, such as maltodextrin DE12.
In the spray drying of the invention, the auxiliary material may be added into the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) in any amount suitable for the invention. Preferably, the auxiliary material is added in an amount of from 20％ to 50％, preferably from 30％ to 40％, based on the weight of solid matter in the fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) .
In an embodiment of the invention, the spray drying of the invention is carried out in a spray dryer. The fermented Rosa Roxburghii juice obtained in the step b) or the concentrated fermentation liquid obtained in the step c) forms solid powders after the spray drying. Any person skilled in the art would be easy to use the solid powders to prepare different types of preparations such as oral liquid, granula, tablets, capsules and pills, etc. according to the actual requirements.
The product with high content of natural vitamin C obtained in the step d) contains natural vitamin C with the content of from 15％ to 30％, and further contains flavone, polyphenol, inositol and SOD which are good for human health, wherein flavone has the content of from 5％ to 15％, polyphenol has the content of from 15％ to 35％, inositol has the content of from 1％ to 3％ and SOD has the content of from 50000 U/g to 150000 U/g.
The process according to the invention uses china-specific Rosa Roxburghii fruit as raw material to produce natural vitamin C products, has mild condition and is easy to operate. Moreover, the product obtained by the process according to the invention has a high content of natural vitamin C while retaining flavone and polyphenol components which are good for human health in Rosa roxburghii, and has significant improvement of antioxidant ability compared with the existing products.
Therefore, the invention also provides a product with high content of natural vitamin C, which is produced according to the above process according to the invention. The product with high content of natural vitamin C has the content of natural vitamin C of more than 15％, even more than 17％ or 20％, while retaining flavone and polyphenol components which are good for human health in Rosa Roxburghii, and has the significant improvement of antioxidant ability compared with the existing products.
In the invention, the content of solid matter is measured as below in accordance with the method for extract as described in the annex X A of the Pharmacopoeia of the People's Republic of China (2010) : precisely weighting a sample； placing the sample in a dry evaporation pan； after drying by distillation in water bash, drying the sample for 3 hours at 105℃； cooling down for 30mins in a dryer； and weighting the dry product quickly and precisely. The content of solid matter (％) of the tested sample is calculated as below: the content of solid matter (％) ＝weight of dry product /weight of the tested sample × 100％.
In the invention, the content of vitamin C is measured by HPLC. HPLC System is Agilent 1200 series (Agilent technologies (China) Co., Ltd., China) , chromatographic column: YMC hydrosphere C18, 5 μm, 150 × 4.6 mm； Mobile phase A: sodium dihydrogen phosphate-phosphoric acid solution (pH 2.0 ± 0.1) , mobile phase B: methyl alcohol； chromatographic condition: 0-5.0 min, B: 0％, 0.8 ml/min； 5.0-5.1 min, B: 70％, 1.2 ml/min； 5.1-9.0 min, B: 70％, 1.2 ml/min； 9.0-9.1 min, B: 0％, 0.8 ml/min； 9.1-20.0 min, B： 0％， 0.8 ml/min； column temperature: 20 ℃； detection wavelength: 243 nm； injection volume: 10 μL.
In the invention, the content of flavone is measured in accordance with the UV-VIS spectrophotometry as described in the annex V A of Pharmacopoeia of the People's Republic of China (2010) , and the content of total polyphenol is measured in accordance with the method for determining tannin content recorded in the annex X B of Pharmacopoeia of the People's Republic of China (2010) , and the content of SOD is measured in accordance with the method for determining SOD activity as described in health food section of GBT5009.171-2003.
In the invention, the content of inositol is measured in accordance with the method for determining inositol as described in the health food section of GB/T 5009.196-2003.
The present invention is further illustrated with reference to the following non-restrictive embodiments.
Example
Example 1: Preparation of Rosa Roxburghii juice from Rosa Roxburghii fruit
Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . 2000.0 Kg of fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, 1280.0 Kg of Rosa Roxburghii juice was obtained. By measurement, the content of solid matter was 8.83％, the content of vitamin C was 1.36％, the content of SOD was 4500 U/g, the content of flavone was 0.90％, the content of inositol was 0.20％ and the content of polyphenol was 2.02％ in the Rosa Roxburghii juice.
Example 2: Preparation of Rosa Roxburghii juice from Rosa Roxburghii pomace-by only using water 
Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, filter cake was obtained. 50.0 g of the filter cake was dipped in an equivalent amount of water (50.0 g) at 30℃. After being immersed for 30 mins, filtration under pressure was carried out to obtain 35.0 g of Rosa Roxburghii juice. By measurement, the content of solid matter was 2.62％, the content of vitamin C was 0.42％, the content of SOD was 1360 U/g, the content of flavone was 0.27％, the content of inositol was 0.06％ and the content of polyphenol was 0.60％ in the Rosa Roxburghii juice.
Example 3: Preparation of Rosa Roxburghii juice from Rosa Roxburghii pomace-by using pectinase
Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, filter cake was obtained. 50.0 g of the filter cake was dipped in 5 eq. (250.0 g) 1.0％ pectinase aqueous solution at 30℃. After being immersed for 30 mins, filtration under pressure was carried out to obtain 216.0 g of Rosa Roxburghii juice. By measurement, the content of solid matter was 1.32％, the content of vitamin C was 0.18％, the content of SOD was 686 U/g, the content of flavone was 0.13 ％, the content of inositol was 0.03％ and the content of polyphenol was 0.30％ in the Rosa Roxburghii juice.
Example 4: Preparation of Rosa Roxburghii juice from Rosa Roxburghii pomace-by using citric acid
Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, filter cake was obtained. 100.0 Kg of the filter cake was dipped in 1.2 eq (120.0 Kg) 0.5％ citric acid aqueous solution at 50℃. After being immersed for 180 mins, filtration under pressure was carried out to obtain 140.0 Kg of Rosa Roxburghii juice. By measurement, the content of solid matter was 7.01 ％, the content of vitamin C was 0.68％, the content of SOD was 3640 U/g, the content of flavone was 0.72％, the content of inositol was 0.16％ and the content of polyphenol was 1.60％ in the Rosa Roxburghii juice.
Example 5: Preparation of Rosa Roxburghii juice from Rosa Roxburghii and pomace
Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . 6000.0 Kg of fresh Rosa Roxburghii fruits were crushed and squeezed. After filtration under pressure, juice and 2440.0 Kg of filter cake was obtained. The filter cake was dipped in 1.2 eq (2928.0 Kg) 0.5％ citric acid aqueous solution at 40℃. After being immersed for 30 mins, filtration under pressure was carried out. Totally 5208.0 Kg of Rosa Roxburghii juice was obtained. By measurement, the content of solid matter was 5.78％, the content of vitamin C was 0.99％, the content of SOD was 3000 U/g, the content of flavone was 0.59％, the content of inositol was 0.13％ and the content of polyphenol was 1.32％ in the Rosa Roxburghii juice.
Example 6: Preparation of concentrated Rosa Roxburghii juice
Fresh Rosa Roxburghii fruits were purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) . Fresh Rosa Roxburghii fruits were crushed and squeezed, After filtration under pressure, 1000.0 mL of Rosa Roxburghii juice was obtained. The Rosa Roxburghii juice was evaporated to obtain 200.0 mL of concentrated Rosa Roxburghii juice at 55℃. After measurement, the content of solid matter was 22.41％, the content of vitamin C was 4.02％, the content of SOD was 11645 U/g, the content of flavone was 2.29％, the content of inositol was 0.51％ and the content of polyphenol was 5.12％ in the juice.
Example 7: Fermentation of Rosa Roxburghii juice by using dry yeast
In a water-bathing constant temperature incubator with vibrator at 30℃, 50 mL of the juice obtained according to the Example 5 was added into a 150 mL fermentation flask. 726 μL 1: 5 (m/v) water activated liquid of high-sugar tolerant dry yeast (Angel Dry Yeast Co., Ltd., China) was added (standing for 30mins at 40℃ before addition) and stirred for 6 hours at the speed of 130 rmp. The yeast was removed by vacuum filtration to give 41 mL of fermented Rosa Roxburghii juice. After measurement, the content of solid matter was 3.29％, the content of vitamin C was 1.03％, the content of SOD was 1711 U/g, the content of flavone was 0.34％, the content of inositol was 0.07％ and the content of polyphenol was 0.75％ in the fermented Rosa Roxburghii juice.
Example 8: Fermentation of Rosa Roxburghii juice by using fresh yeast
In a water-bathing constant temperature incubator with vibrator at 30℃, 50 mL of the juice obtained according to the Example 5 was added into a 150 mL fermentation flask. 220 μL 1: 1 (m/v) aqueous solution of high-sugar tolerant dry yeast (Angel Dry Yeast Co., Ltd., China) was added and stirred for 6 hours at the speed of 130 rmp. The yeast was removed by vacuum filtration to give 45 mL of fermented Rosa Roxburghii juice. After measurement, the content of solid matter was 3.23％, the content of vitamin C was 0.99％, the content of SOD was 1802 U/g, the content of flavone was 0.33％, the content of inositol was 0.07％ and the content of polyphenol was 0.72％ in the fermented Rosa Roxburghii juice.
Example 9: Fermentation of purchased Rosa Roxburghii juice
In a water-bathing constant temperature incubator with vibrator at 30℃, 100 mL of juice purchased from Guizhou Shanzhenbao Green Technology Development Co., Ltd. (Guizhou, China) was added into a 250 mL fermentation flask, wherein the content of solid matter was 25％ and the content of vitamin C was 4.31％. Then 6875 μL 1: 5 (m/v) water activated liquid of high-sugar tolerant dry yeast (Angel Dry Yeast Co., Ltd., China) was added (standing for 30 mins at 40℃  before addition) , and stirred for 6 hours at the speed of 130 rmp. The yeast was removed by vacuum filtration to give 87 mL of fermented Rosa Roxburghii juice. After measurement, the content of solid matter was 14.03 ％, the content of vitamin C was 4.10 ％, the content of SOD was 12995 U/g, the content of flavone was 1.95 ％, the content of inositol was 0.32％ and the content of polyphenol was 4.13％ in the fermented Rosa Roxburghii juice.
Example 10: Fermentation of the concentratedRosa Roxburghii juice
In a water-bathing constant temperature incubator with vibrator at 30℃, 50 mL of the juice obtained according to the Example 6 was added into a 150 mL fermentation flask. 3360 μL 1: 5 (m/v) water activated liquid of high-sugar tolerant dry yeast (Angel Dry Yeast Co., Ltd., China) was added (standing for 30mins at 40℃ before addition) and stirred for 6 hours at the speed of 130 rmp. The yeast was removed by vacuum filtration to give 42 mL of fermented Rosa Roxburghii juice. After measurement, the content of solid matter was 16.29％, the content of vitamin C was 3.83％, the content of SOD was 11649 U/g, the content of flavone was 1.75％, the content of inositol was 0.37％ and the content of polyphenol was 3.70％ in the fermented Rosa Roxburghii juice.
Example 11: Spray drying of the fermented Rosa Roxburghii juice
0.5 g maltodextrin DE12 (Roquette (China) Co., Ltd., China) was added into 38 mL of the fermented Rosa Roxburghii juice obtained according to the Example 10, stirred until complete dissolution, and then dried by BUCHI B290 spray dryer (Buchi Co., Ltd., Switzerland) . The conditions for drying were: (1) inlet temperature: 135℃； (2) rate of feed: 5-10 ml/min； (3) rate of supplying air: 400-600 l/h； (4) air velocity: 30-40 m3/h. 5.4 g natural vitamin C powder was obtained after drying. The yield was calculated according to the below formula:
Yield ＝ (weight of natural vitamin C powder/ (weight of solid matter in Rosa Roxburghii juice + weight of maltodextrin DE12) ) × 100
After calculation, the yield was 78％. After measurement, the content of vitamin C was 21.46 ％, the content of SOD was 115753 U/g, the content of flavone was 9.95 ％, the content of inositol was 2.28 ％ and the content of polyphenol was 22.70 ％ in the obtained natural vitamin C powder.
Example 12: Spray drying of the fermented Rosa Roxburghii juice
0.6 g maltodextrin DE12 (Roquette (China) Co., Ltd., China) was added into 45 mL of the fermented Rosa Roxburghii juice obtained according to the Example 8, stirred until complete dissolution, and then dried by BUCHI B290 spray dryer (Buchi Co., Ltd., Switzerland) . The conditions for drying were: (1) inlet temperature: 135℃； (2) rate of feed: 5-10 ml/min； (3) rate of supplying air: 400-600 l/h； (4) air velocity: 30-40 m3/h. 1.7 g natural vitamin C powder was obtained after drying. The yield was calculated as the Example 11, and it was 83％. After measurement, the content of vitamin C was 19.04 ％, the content of SOD was 95640 U/g, the content of flavone was 7.62 ％, the content of inositol was 1.94 ％ and the content of polyphenol was 20.53％ in the obtained natural vitamin C powder.
Example 13: Spray drying of the fermented Rosa Roxburghii juice
2.2 g maltodextrin DE12 (Roquette (China) Co., Ltd., China) was added into 100 mL of the fermented Rosa Roxburghii juice obtained according to the Example 9, stirred until complete dissolution, and then dried by BUCHI B290 spray dryer (Buchi Co., Ltd., Switzerland) . The  conditions for drying were: (1) inlet temperature: 135℃； (2) rate of feed: 5-10 ml/min； (3) rate of supplying air: 400-600 l/h； (4) air velocity: 30-40 m3/h. 10.1 g natural vitamin C powder was obtained after drying. The yield was calculated as the Example 11, and it was 62％. After measurement, the content of vitamin C was 20.13 ％, the content of SOD was 72993 U/g, the content of flavone was 10.38 ％, the content of inositol was 2.01 ％ and the content of polyphenol was 23.69％ in the obtained natural vitamin C powder.
Example 14: Vacuum freeze drying of the fermented Rosa Roxburghii juice
100 g of the fermented Rosa Roxburghii juice obtained according to the Example 5 was pre-freezed completely in a refrigerator at -80℃, and then was placed into a freeze dryer alpha 2-4 LST plus (Martin Christ Gefriertrocknungsanlagen GmbH, German) for drying for 24 hours. 3.5 g natural vitamin C freeze-dried powder was obtained after drying, wherein the content of vitamin C was 29.5％.
Example 15: Assessment of antioxidant capacity
The antioxidant capacity of the Rosa Roxburghii powder obtained according to the Example 13 (the content of vitamin C was 20.13％) , commercially available acerola cherry powder (the content of vitamin C was 16.72％) and synthetic vitamin C (the content of vitamin C was adjusted to 22.90％ by using maltodextrin DE12) was assessed by the hydrophilic antioxidant capacity -antiperoxyl radicals method. In this method, the capacity resisting to external ROO-was determined with AAPH as resource of producing ROO-, fluorescein as fluorescence probe and vitamin E water soluble analogue Trolox as standard contrast. (see Ou B et al, J Agric and Food Chem, 2001, 49 (10) : 4619-4926； Huang D et al, J Agric and Food Chem, 2002, 50 (7) : 1815-1821； and American patent US7,132,296B2) The results showed that, the hydrophilic antioxidant capacity of the Rosa Roxburghii powder, acerola cherry powder which was commercially available and synthetic vitamin C was 2758, 818 and 717 μmole TE/g, respectively, i.e. the antioxidant capacity of natural vitamin C powder prepared by the process of the invention was more than 3 times of other samples.

Claims (10)

  1. A process for producing a product with high content of natural vitamin C, comprising:
    a) Obtaining Rosa Roxburghii juice；
    b) Fermenting the Rosa Roxburghii juice with yeast to obtain fermented Rosa Roxburghii juice；
    c) Optionally, concentrating the fermented Rosa Roxburghii juice to obtain concentrated fermented juice； and
    d) Drying the fermented Rosa Roxburghii juice or the concentrated fermented juice to provide the product with high content of natural vitamin C.
  2. The process of claim 1, wherein in the step a) the Rosa Roxburghii juice is obtained by direct squeeze of Rosa Roxburghii fruits and/or extracting pomace produced from the squeeze.
  3. The process of claim 1, wherein the Rosa Roxburghii juice obtained in the step a) contains 1-10％ by weight of solid matter and 0.1-2.5％ by weight of vitamin C.
  4. The process of claim 1, wherein the step a) further comprises a step of concentrating the obtained Rosa Roxburghii juice, for instance, by 2-10 times, preferably 3-6 times such as 3, 4 or 5 times.
  5. The process of claim 1, wherein the yeast is selected from the group consisting of baker’s yeast (Saccharomyces cerevisiae) , brewing yeast (Saccharomyces bayanus, Saccharomyces cerevisiae or Saccharomyces uvarum) , specific functional yeast, Hansenula spp. and Rhizopus oryzae.
  6. The process of any one of claims 1-5, wherein the yeast is used in an amount of 4-10％, preferably 6％, based on the weight of the solid matter in the Rosa Roxburghii juice.
  7. The process of any one of claims 1-5, wherein the fermentation is carried out at 10-50℃, preferably 20-40℃, more preferably 30℃.
  8. The process of claim 1, wherein the concentrated fermented juice obtained in the step c) contains 1-25％ by weight of solid matter, and 0.2-6％ by weight of vitamin C.
  9. The process of claim 1, wherein the drying of the step d) is spray drying.
  10. A product with high content of vitamin C produced according to the process of any one of the claims 1-10.
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