WO2016122995A1 - Composition antibactérienne de nanoparticules d'argent liées à un agent de dispersion - Google Patents

Composition antibactérienne de nanoparticules d'argent liées à un agent de dispersion Download PDF

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WO2016122995A1
WO2016122995A1 PCT/US2016/014665 US2016014665W WO2016122995A1 WO 2016122995 A1 WO2016122995 A1 WO 2016122995A1 US 2016014665 W US2016014665 W US 2016014665W WO 2016122995 A1 WO2016122995 A1 WO 2016122995A1
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nps
antibacterial composition
antibacterial
dispersing agent
composition according
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PCT/US2016/014665
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English (en)
Inventor
Zoe Vineth Quinones JURADO
Miguel Angel Waldo MENDOZA
Luis Manuel Cespedes COVARRUBIAS
Hugo Marcelo Aguilera BANDIN
Jose Elias Perez LOPEZ
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A. Schulman, Inc.
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Publication of WO2016122995A1 publication Critical patent/WO2016122995A1/fr
Priority to CONC2017/0008550A priority Critical patent/CO2017008550A2/es

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00—Nanotechnology for materials or surface science, e.g. nanocomposites
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
    • A01N55/02—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms

Definitions

  • the present invention relates to an antibacterial composition containing silver nanoparticles (Ag NPs) bonded to the surface of a ceramic substrate dispersing agent, in which Ag NPs have a photocatalytically inactive surface, being bactericidal in the dark as well as also under Ultraviolet and visible light (UV-vis).
  • Ag NPs silver nanoparticles
  • UV-vis Ultraviolet and visible light
  • Silver compositions commonly used as antimicrobial are silver sulphadiazine (CioH9N402SAg), silver acetate (AgCH3COO), silver nitrate (AgN03), other silver salts, or other compositions based on silver ions caught in vitreous structures, of zeolites, clays, etc.
  • metallic silver as nanometric size particles Ag NPs
  • S. Pal et al. [Applied and Environmental Microbiology, 73 (2007) 1712-1720] and N. Ayala et al. [Nanobiotechnology, 5 (2009) 2-9] have referred that at a smaller size of the particle, the superficial area increases and consequently, a greater antimicrobial effect is developed.
  • the presentation of the Ag NPs consists of a humid paste of flocculated particles and their re-dispersion with another material will depend on the mixing by means of mechanical work.
  • compositions where silver is "supported” on the ceramic surface are known as "supported, decorated or core-shell" when atoms of a constituent are adhered to the exterior of a support substrate, either in form of coating or of particles with nanometric dimension homogeneously distributed on the support.
  • any other system can get excited by absorption of photons with a characteristic frequency, or also by means of heat or electricity.
  • antibacterial compositions of noble metals supported to a semiconductor like Ag/Ti02 require photo-excitation to perform efficiently as bactericidal, however, these are not effective in the dark and require to be supplemented with other antibacterial substances.
  • the Ag/Ti02 photocatalytic compound is combined with zinc chloride plus hydroxyapatite and in the patent JPH11192290 other metallic oxides such as silica are combinedr
  • the patent application MX MX/a/2010/010434 refers to a Ag/Ti02 antibacterial compound "supported" by way of aqueous suspension and whose silver nanoparticle size is controlled through the use of Gallic acid in dimensions between 20-60 nm, in which the Ag NPs content that is supported, is only of approximately 5% of the weight in order to maintain the stability of the suspension of the Ag/Ti02 compound, and therefore, given the low Ag NPs content, it may not perform as bactericidal under non-photocatalytic conditions.
  • Document KR20090035812 describes an antimicrobial that consists of the combination of metallic nanoparticles or metallic oxides encapsulated with ⁇ 2 for photocatalytic use. This composition could only develop bactericidal activity by the generation of reactive species of (ROS) oxygen when there is excitation with UV/visible light.
  • ROS reactive species of
  • Document R0126368 refers to silver nanostructured compounds supported on oxides like ZnO and ⁇ 2, with a silver weight content of less than 6% and with antimicrobial activity based on the photocatalytic effect.
  • Document KR20010057595 refers to a silver-coated T1O2 antibacterial photocatalyst, with a content of silver less than 5% in weight regarding the total composition, which is a low content to perform efficiently as bactericidal in the dark.
  • the present invention refers to an antibacterial composition based on Ag nanoparticles "supported” to a ceramic substrate, photocatalytic (semiconductor material) or non photocatalytic, in which Ag NPs have a photocatalytically inactive surface being bactericidal in the dark, as well as under Ultraviolet and visible light (UV- vis).
  • an adherent substrate of the silver nanoparticles helps to prevent their agglomeration, besides optimizing the exposition of the surface area of such Ag NPs, giving as a result that the substrate used in the present invention performs as dispersing agent to ensure greater contact of the antimicrobial active (Ag NPs) with the possible bacteria during its application.
  • the substrate or dispersing agent comprises one or more of the ceramic particles selected among the semiconductors such as Titanium dioxide (T1O2), Zinc oxide (ZnO), Silicon dioxide (S1O2) or even particles of non-semiconductor ceramics such as; Zirconium dioxide (Zr02), Aluminum trioxide (AI2O3), aluminosilicates (S1O2.AI2O3), Tin dioxide (Sn02), Tin oxide (SnO), Copper oxides (CuO, CU2O), Antimony oxide (Sb,Os), Beryllium oxide (BeO), Tellurium oxide (Te02), Indium oxide ( ⁇ 2 ⁇ 3), Gallium oxide (Ga203), and their respective hydroxides.
  • the semiconductors such as Titanium dioxide (T1O2), Zinc oxide (ZnO), Silicon dioxide (S1O2) or even particles of non-semiconductor ceramics such as; Zirconium dioxide (Zr02), Aluminum trioxide (AI2O3), aluminosilicate
  • the present invention refers to an antibacterial composition containing silver nanoparticles (Ag NPs) "supported” to the surface of a dispersing agent having bactericidal action against Gram negative and Gram positive bacteria, which does not require photo excitation to destroy bacteria, since neither the silver nanoparticles (Ag NPs) or the dispersing agent are of interest by their photocatalytic activity, because their performance is required without the condition of photo excitation.
  • the antibacterial agent (Ag NPs) is characterized to have a photocatalytically inactive surface under visible light.
  • the present invention is related to an antibacterial composition containing silver nanoparticles (Ag NPs) bonded to the surface of a dispersing agent, characterized because Ag NPs have a high superficial area (Figure 3), but not a photocatalytically surface (Figure 4).
  • Ag NPs silver nanoparticles
  • the antibacterial composition of the present invention mainly acts through the mechanism of permeability and breaking of the cellular membrane at the contact with the antibacterial agent (Ag NPs), in which the surface of silver nanoparticles (Ag NPs) is characterized to have a photocatalytically inactive surface under visible light.
  • the antibacterial composition constituted by Ag NPs of passive character must be constituted with a content of 8.5% or of up to 70% in weight regarding the total composition to ensure the bactericidal character in the dark, as well as also under Ultraviolet and visible light (UV-vis).
  • the antibacterial composition is compatible to be mixed with polymer, adhesive, metal and ceramic materials, for applications as for example: materials suitable for medical products, surfaces of appliances, in ventilation systems or air purification, carpets, synthetic grass, paper, fibers for textiles, non-woven fabric, articles in contact with food, kitchen utensils, plastic films, toilet articles, etc.
  • FIGURE 1 It shows a qualitative spectrum of the elemental chemical analysis of the Ag Ti02 "supported” compound corresponding to the formulation 14, obtained by energy-dispersive X-ray spectroscopy (EDX). In axis “X” is shown the energy of the photon emission (keV) and in T the counting of each emitted radiations.
  • EDX energy-dispersive X-ray spectroscopy
  • FIGURE 2 It shows a micrograph of the silver nanoparticles compound (Ag NPs) deposited on the surface of the titanium dioxide (T1O2) dispersing agent, corresponding to the Ag/Ti02 "supported” compound obtained from formulation 1 , which does not show bactericidal activity in the dark. Obtained through transmission electron microscopy (TEM), seen at 100000x magnification.
  • TEM transmission electron microscopy
  • FIGURE 3 It shows a micrograph of the silver nanoparticles compound- (Ag NPs) deposited on the surface of the titanium dioxide (T1O2) dispersing agent, which shows bactericidal activity in the dark.
  • the Ag/Ti02 "supported" compound corresponds to formulation 14.
  • the micrograph was obtained through transmission electron microscopy (TEM), seen at 100000x magnification.
  • FIGURE 4 It shows the absorption spectra of Ultraviolet and visible light (UV-vis) of Ag/Ti02 "supported” compound, with different content in weight percentage of the Ag NPs in a range approximately between 1.5-5%, obtained from formulation 3, formulation 6 and formulation 7, that correspond to the compound; (1.5Ag/Ti02), (4Ag/Ti02) and (5Ag Ti02), respectively, using as controls both the pure titanium dioxide T1O2 spectrum and the spectrum of the pure silver nanoparticles Ag NPs.
  • X is shown the excitation energy of the different materials (nm) and in axis ⁇ " is shown the intensity of absorbed energy (L mo crrr 1 ).
  • Antibacterial composition Substance used in the control of the growth and/or destruction of bacteria.
  • Bactericidal Antibacterial substance able to kill bacteria
  • Substrate or dispersing agent Surface of the ceramic particles used as dispersing agent of the silver nanoparticles.
  • Silver nanoparticles (Ag NPs): Metallic silver particles of nanometric size.
  • Photocatalytic activity / photocatalysis Effect on the electrons mobility in certain substances when exposed to the light, as for example; when titanium dioxide (T1O2) is irradiated with Ultraviolet light (UV), it increases the electrons mobility.
  • T1O2 titanium dioxide
  • UV Ultraviolet light
  • Plasmon Effect Is the phenomenon of the collective oscillation of electrons maintained on the surface of a metal; it originates by the incidence of photons that remain caught by free electrons and generates more reactivity on the metal surface.
  • the present invention is related to an antibacterial composition that comprises an antibacterial agent consisting of silver nanoparticles (Ag NPs) which show a quasi- spherical structure and are bonded to the surface of a dispersing agent that prevents
  • the present invention refers to antibacterial composition in which silver nanoparticles (Ag NPs) have an average diameter approximately smaller than 15 nm; which makes them photocatalytically inactive due to a confinement of electrons on the surface, related to the small size of the Ag NPs; likewise, Ag NPs do not form agglomeration, that is to say, these are dispersed and homogeneously distributed on the on the surface of the dispersing agent.
  • Ag NPs silver nanoparticles
  • the ceramic substrates is are not used by the photocatalytic activity that it may develop under UV light, but these are interest as dispersing agent of the silver nanoparticles (Ag NPs), since they behaves as a substrate with capacity to adhere Ag NPs in a massive and homogeneous way.
  • the route through which Ag NPs is formed is by means of the attraction of positive silver ions in the places with Oxygen vacancies (O-) of the ceramic substrate, followed by the size growth of the Ag NPs due to the accumulation of silver atoms through a chemical reduction reaction.
  • O- Oxygen vacancies
  • the antibacterial agent is homogeneously supported on the surface of the ceramic substrate to favor the contact of the Ag NPs with the bacteria, in which such particles are characterized to have an average diameter of approximately less than 15 nm, as shown in figure 3.
  • the invention is defined to be an antibacterial composition against Gram positive bacteria such as Staphylococcus aureus, Staphylococcus epideimidis, Staphylococcus saprophyticus, Enterococcus, pyogenes, Enterococcus faecalis, Lactobacillus sp. and Gram negative, such as Escherichia coli, S., Salmonella, Pseudomonas aeruginosa, among others.
  • Gram positive bacteria such as Staphylococcus aureus, Staphylococcus epideimidis, Staphylococcus saprophyticus, Enterococcus, pyogenes, Enterococcus faecalis, Lactobacillus sp.
  • Gram negative bacteria such as Staphylococcus aureus, Staphylococcus epideimidis, Staphylococcus saprophyticus, Enterococcus, pyogenes, Enterococcus
  • the invention of the antibacterial composition has preferably a concentration of Ag NPs with average diameter approximately lower than 15 nm in the range between approximately from 8.5 to 70% in weight regarding the total composition.
  • the antibacterial composition of the present invention provides a bactericidal effect through the permeation in the cellular membrane of bacteria, this caused by the effect of the high exposition of the superficial area of the silver nanoparticles (Ag NPs) and, additionally by the electrostatic destabilization of the cellular membrane to the Gram negative bacteria, caused by the presence of ionized silver.
  • the antibacterial composition is constituted with the Ag Ti02 "supported” compound, which is elaborated in an aqueous medium by means of the adhesion of Ag NPs to the titanium dioxide (T1O2) surface, this upon the Oxygen vacancies (0-) in the interface of the T1O2 substrate.
  • Table 1 shows different formulations used to obtain the Ag/Ti02 "supported” compound.
  • Table 1 shows examples of formulations used to obtain the Ag/TiC "supported" compound, in which the numeric value preceding the nomenclature Ag/Ti02, refers to the maximum percentage in weight of the silver to be supported. starting from the AgN03 precursor and, for some compounds is compared with the real content of Ag NPs that could be supported.
  • Figure 1 shows a qualitative spectrum of the elementary chemical analysis acquired by energy-dispersive X-ray spectroscopy (EDX), corresponding to the 25Ag/Ti02 compound, obtained from the formulation 14 identified as one of the “supported” compounds that showed bactericidal activity without the photo-excitation of (UV - vis) light.
  • EDX energy-dispersive X-ray spectroscopy
  • Figure 1 shows a qualitative spectrum of the elementary chemical analysis acquired by energy-dispersive X-ray spectroscopy (EDX), corresponding to the 25Ag/Ti02 compound, obtained from the formulation 14 identified as one of the “supported” compounds that showed bactericidal activity without the photo-excitation of (UV - vis) light.
  • X energy of the photon emission
  • Y the counting of each emitted radiation.
  • This X-ray emission spectrum indicates the X-ray energy of each one of the elements
  • the antibacterial composition is formed from the element of Silver (Ag) and Titanium (Ti).
  • the Copper (Cu) sign is due to the grate in which the compound was supported for its analysis.
  • Example 3 Comparative of the structure of Ag/Ti02 "supported" compound with and without bactericidal activity in the dark, seen at 100,000x magnification.
  • FIGS 2 and 3 show the images of the Ag/Ti02 "supported" compound structure, which is formed from quasi-spherical silver nanoparticles (Ag NPs) with an average diameter approximately smaller than 5 nm, bonded on the titanium dioxide (T1O2) surface, where T1O2 is present as a substrate or dispersing agent that supports Ag NPs keeping them separate, thus achieving the exposition of the superficial area of the silver nanoparticles (Ag NPs), and therefore providing more contact of the antibacterial agent consisting of (Ag NPs) with the bacteria during its application.
  • Ag NPs quasi-spherical silver nanoparticles
  • T1O2 titanium dioxide
  • Figure 2 presents the structure of the 0.5Ag TiO2 compound obtained with formulation 1 , which does not have bactericidal activity in the dark due to the low content of Ag NPs on the T1O2 dispersing agent, and it is compared with the structure of the 25Ag/Ti02 compound obtained from formulation 14 ( Figure 3), in which occurred bactericidal activity in the dark; this last one is formed from the T1O2 dispersing agent with 14.1 % in weight of the Ag NPs homogeneously distributed.
  • Figure 4 shows the absorption spectra of Ultraviolet radiation and visible light (UV-vis) of the Ag Ti02 "supported" compounds, obtained from formulations 3, 6 and 7 identified as 1.5 Ag Ti02, 4 Ag/TiCte and 5 Ag Ti02, using as controls both the pure titanium dioxide T1O2 spectrum and the silver nanoparticles Ag NPs spectrum.
  • UV-vis visible light
  • axis "X” is shown the wavelength corresponding to the excitation energy (nm) and in the axis "Y” is shown the intensity of absorbed energy (L mol-'cnr 1 ).
  • the electromagnetic spectrum is the set of wavelengths of the whole electromagnetic radiation; it is formed by gamma rays, X-rays, ultraviolet radiation, visible light and infrared radiation, and others of greater wavelength.
  • the wavelength is defined as the distance that energy travels in a time equivalent to a period.
  • An electromagnetic wave is formed by photons. The energy of each photon is directly proportional to the frequency of the wave. The higher the frequency is the larger is the quantity of energy contained in each photon. It happens that the frequency increases as the wavelength decreases, and vice versa.
  • the absorption spectra of UV-vis light that form the image of Figure 4 show that in pure state, T1O2 presents photocatalytic activity in the Ultraviolet range and the Ag NPs in the range of visible light.
  • the bond of Ag NPs with average diameter approximately smaller than 15 nm to the T1O2 surface in the Ag/Ti02 "supported" compound modifies the energy of the frequency so that T1O2 photocatalysis may occur.
  • the T1O2 dispersing agent changes the absorption of energy of a 283.5 nm wavelength to a value of 334 nm, reason why it is required the absorption of a frequency of less energy to be photocatalytic.
  • This wavelength sliding indicates the formation of an interface between Ag NPs and T1O2, where there is an increment in the mobility of the electrons, just as it has been reported in the literature and as it is shown in the absorption spectra of UV-vis light of the compounds; 1.5 Ag/Ti02, 4 Ag/Ti02 and 5 Ag/Ti02 as shown in Figure 4.
  • the type of Ag Ti02 antimicrobials based on the photocatalytic technology presents an electromagnetic phenomenon characteristic of Ag NPs, in which, when being irradiated in the range of the visible spectrum, the reactivity by the mobility of
  • Example 5 Microbiological analysis to determine the minimum bactericidal concentration (MBC) under the dark of the Ag TiCte "supported 1 ' compound.
  • the minimum bactericidal concentration (MBC) corresponds to the smallest concentration capable to kill 99,9% of bacterial population.
  • the antimicrobial susceptibility testing consisted of a dilution method based on the NCCLS International Standards.
  • the bactericidal activity of the Ag/TiCte "supported” compounds is mentioned in Table 1 in which the Example 1 was evaluated by means of the study of the minimum bactericidal concentration (MBC) and with it determined the minimum percentage of the Ag NPs mass with average diameter approximately smaller than 15 nm that should be adhered to the T1O2 dispersing agent, and the minimum concentration of the Ag/Ti02 "supported” compound in which the antibacterial composition acts as bactericidal in the dark and, consequently, under Ultraviolet and visible light (UV-vis).
  • MBC minimum bactericidal concentration
  • a Luria Bertani type liquid medium was prepared to cultivate bacteria: 1.0% of tryptone, 0.5% of yeast extract and 1.0% of NaCI.
  • the culture medium was placed in test tubes to a volume of 3mL and it was mixed with the Ag/Ti02 compound in quantities from 0.5 - 20.0 mg/mL, respectively.
  • control tests were parallel performed at these same concentrations of that tested for the nanocomposition. Titanium dioxide (T1O2) used as
  • dispersing agent was also analyzed in the same concentration range to determine the antibacterial capacity it contributes in a dark environment.
  • the mixtures contained in the test tubes were sterilized at 121°C during 15 minutes.
  • Inoculum were prepared in liquid medium of Gram negative bacteria; Salmonella sp; Escherichia coli ATCC 25922 (American Type Culture Collection, Rockville, Md.), as well as of the Gram positive bacteria Staphylococcus aureus ATCC 25923.
  • Bacteria were added to the culture medium mixtures and the different Ag/TiCte compounds, obtained from the formulas presented in Table 1, maintaining a concentration between 10 7 and 10 6 CFU/mL respectively.
  • bacteria were incubated in the dark, during the night inside an autoclave, by 10 h at 37°C arid 150 rpm.
  • MBC analysis by means of massive striation in Luria Bertani Agar plates with 100 L of sub- cultivation of each tube. The plates were incubated during 24 h at 37°C to determine the final points of the minimum bactericidal concentration MBC, which is a measure indicating the use of the smallest concentration of the Ag/TiCte "supported" compound for not having presence of bacteria after the incubation.
  • the analysis was made by triplicated for each sample in the different concentrations of 0.5 - 20.0 mg/mL.
  • the MBC microbiological analysis for pure titanium dioxide (Ti02) used as dispersing agent was performed to determine the bactericidal capacity contributed by this dispersing agent in the dark or visible light. As a result it was obtained that within the concentration range of 0.5 - 20.0 mg/mL there was bacteria growth. That corroborates the absorption spectrum of UV-vis light of the Figure in the example 4, where the T1O2 substrate could only have bactericidal activity when exposed to UV radiation.
  • Example 7 Ag/TiCte "supported" compounds that did not show bactericidal activity in the dark.
  • the Ag Ti02 compounds formed with a content of silver nanoparticles (Ag NPs) lower than 8.5% in weight with regard to the total of the composition were not able to destroy bacteria such as: Salmonella; Escherichia coli and Staphylococcus aureus. This indicates that the silver nanoparticles (Ag NPs) photocatalytically inactive should be present in a quantity enough to perform in sharp cutting way to reach permeability and breaking of the bacteria membrane and achieve their complete elimination.
  • Example 8 Ag Ti02 "supported” compounds with bactericidal activity even in the dark.
  • the Ag/TiC compounds with a minimum Ag NPs percentage of 8.5% in weight with regard to the total of the composition present bactericidal activity, even using a dosage of 10 mg/mL they eradicate bacteria cultures concentrated between 10 7 to 10 6 CFU/mL.
  • five Ag Ti02 "supported" compounds are shown in Table 4 as example of bactericidal compositions
  • Gram negative bacteria such as: Salmonella Escherichia coli and Gram positive bacteria, as for example: Staphylococcus aureus.
  • the five Ag/TiCte "supported" compounds shown in Table 4 are formed with Ag NPs with an average diameter of approximately less than 15nm, just as it is illustrated in Figure 3 for the Example 3.
  • Ag NPs with that diameter and adhered to the T1O2 substrate are photocatalytically inactive as shown in Figure 4, so these require to be present in the antibacterial composition at a concentration in the range of approximately 8.5 to 70% in weight with regard to the total of the composition to generate bactericidal
  • Table 4 shows how the Gram negative and positive bacteria culture, concentrated between 10 7 to 10 e CFU/ml, is eradicated with a minimum bactericidal concentration ( BC) of 10 mg per mL of the culture, this using an antibacterial composition that fulfills the minimum content of 8.5% in weight with regard to the total Ag NPs composition with average diameter approximately lower than 15 nm.
  • BC bactericidal concentration
  • Such antibacterial composition generates more destruction of Gram negative bacteria, for example the MBC value against Gram negative Salmonella bacteria is of 7 mg/mL and with the Gram negative Escherichia coli is achieved the MBC value with up to 4 mg/mL of an antibacterial composition having a concentration of 18.5% in weight regarding the total of the composition.
  • Example 9 Substrates that can be used as Ag NPs adhesion and dispersing agents.
  • the dispersing agent or substrate that can be used as support to the (Ag NPs) antibacterial agent are particles of semiconductors such as Titanium dioxide (T1O2), Zinc oxide (ZnO), Silicon dioxide (S1O2) or even particles of non-semiconductor ceramics such as Zirconium dioxide (ZrCte), Aluminum trioxide (AI2O3), aluminosilicates (S1O2.AI2O3), Tin dioxide (SnC ), Tin oxide (SnO), Antimony oxide (Sb ⁇ Os), Beryllium
  • BeO Tellurium oxide
  • Te02 Indium oxide
  • ItoCb Indium oxide
  • CuO Copper oxide
  • CU2O Gallium oxide
  • GazOs Gallium oxide
  • the common characteristic of the support is that these are materials that may have an electro-attractive surface to positive ions (for example, silver ions).
  • positive ions for example, silver ions.
  • the attraction of silver ions and formation of silver nanometric particles on the dispersing agent originates starting from vacancies (0-) formed by the deprotonation of hydroxyl groups (-OH) of the surface of the substrate. Therefore, the formation of silver nanometric particles on the dispersing agent depends on the alkaline condition able to promote a larger number of vacancies (0 ) on the surface of the substrate.
  • the condition of the hydrogen potential (pH) during the reduction-deposition reaction during the formation of the composition that consists of the dispersing agent supporting Ag NPs with average diameter approximately lower than 15 nm will be characteristic for each type of substrate.
  • Table 5 shows the relation of the pH value required to generate the largest deprotonation of the surface of some metallic oxides and hydroxides mentioned as part of the invention.
  • the plastic films were fabricated with a material of polyethylene and the antimicrobial composition at a concentration of 100 to 600 ppm, using a melt blender and film extruder (Killion model D.S. Winder).
  • Antimicrobial composition performance integrated into a matrix that require antimicrobial activity will be dependent of the de-agglomerating distribution and uniform dispersion.
  • Standard JIS Z 2801 specifies the efficacy on bacteria on the surface of antimicrobial products
  • the inoculum was prepared using Escherichia coli ATCC # 8739. Dilute nutrient broth prepared as described in the test method was used to further dilute the inoculum to a target starting concentration of 2.5- 0 x 10 5 CFU/mL.
  • Each sample piece was placed in a sterile Petri dish and then was inoculated with 0.4 mL of the inoculum. The inoculum was then covered the sterile plastic in order to spread the inoculum evenly over the sample surface and hold it in place.
  • the samples and controls were incubated for 24 hours at 35°C and a relative humidity of at least 90%.
  • 10.0 mL of neutralizing broth (SCDLP) was added to the Petri dish.
  • the Petri dish containing the test pieces and the SCDLP was then placed onto a shaker and mixed thoroughly to facilitate the release of the inoculum from the sample surface into the neutralizing broth.
  • Serial dilutions of the neutralizing broth containing the inoculum were plated. All plates were incubated at 35°C for 24- 48 hours. After incubation, bacterial colonies were counted and recorded.
  • Results can be found in the data tables below. The results pertain only to samples tested.
  • the number of viable bacteria in the test inoculum was 6.8 x 10 5 CFU/mL. This is the initial number of bacteria of the starting inoculum.
  • the value of the antimicrobial activity was calculated according to the formula listed below and recorded as log reduction.
  • Percent reductions are determined by comparing the sample after the contact time to the untreated control sample after the contact time. Percent reduction is translated into log reduction:

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Abstract

La présente invention concerne une composition antibactérienne contre des bactéries à Gram positif et Gram négatif, qui contient des nanoparticles d'argent (NP d'Ag) présentant un diamètre moyen d'approximativement moins de 15 nm qui ont une surface photocatalytiquement inactive, présentes dans une plage comprise entre 8,5 et jusqu'à 70 % en poids par rapport à la composition totale, dans laquelle les NP d'Ag sont l'agent antibactérien qui demeure exposé de manière homogène par un agent de dispersion en céramique tel que TiO2 et son activité bactéricide est générée en présence de la lumière UV-visible et/ou à l'obscurité.
PCT/US2016/014665 2015-01-26 2016-01-25 Composition antibactérienne de nanoparticules d'argent liées à un agent de dispersion WO2016122995A1 (fr)

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Cited By (4)

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Publication number Priority date Publication date Assignee Title
CN108976475A (zh) * 2018-07-18 2018-12-11 安徽江淮汽车集团股份有限公司 一种抗菌剂及其制备方法
CN113402169A (zh) * 2021-07-14 2021-09-17 佛山市陶莹新型材料有限公司 一种易洁的抛釉及其制备方法
WO2022031637A1 (fr) * 2020-08-04 2022-02-10 Kuprion Inc. Applications antiseptiques d'agglomérats de nanoparticules métalliques
CN116459386A (zh) * 2023-03-30 2023-07-21 西北大学 一种载银纳米二氧化钛修饰聚多巴胺光热抗菌型水凝胶及其制备方法

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CN108976475A (zh) * 2018-07-18 2018-12-11 安徽江淮汽车集团股份有限公司 一种抗菌剂及其制备方法
WO2022031637A1 (fr) * 2020-08-04 2022-02-10 Kuprion Inc. Applications antiseptiques d'agglomérats de nanoparticules métalliques
CN113402169A (zh) * 2021-07-14 2021-09-17 佛山市陶莹新型材料有限公司 一种易洁的抛釉及其制备方法
CN116459386A (zh) * 2023-03-30 2023-07-21 西北大学 一种载银纳米二氧化钛修饰聚多巴胺光热抗菌型水凝胶及其制备方法
CN116459386B (zh) * 2023-03-30 2024-04-26 西北大学 一种载银纳米二氧化钛修饰聚多巴胺光热抗菌型水凝胶及其制备方法

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