WO2016119274A1

WO2016119274A1 - Mosquito capturing method and attractant device used in same

Info

Publication number: WO2016119274A1
Application number: PCT/CN2015/072477
Authority: WO
Inventors: 刘宪雄
Original assignee: 刘宪雄
Priority date: 2015-01-28
Filing date: 2015-02-09
Publication date: 2016-08-04
Also published as: CN104719268A

Abstract

A mosquito capturing method, comprising: arranging an attractant device (20) for placing an attractant capable of being volatilized to form an odor bait at an air inlet (401) of a mosquito capturing device (10), attracting the mosquito to the area by utilizing the odor bait formed via volatilization of the attractant, and then drawing in the mosquito into an internal portion of the mosquito capturing device (10) by utilizing negative pressure air flow generated by the mosquito capturing device to capture and kill the mosquito. The attractant used in the attractant device provided in the method comprises three different volatilizable substances respectively arranged in three spaces isolated from each other to form the odor bait attracting the mosquito. The odor formed via the volatilization of the three different substances in the attractant can attract mosquitos and insects to an effective working area of the mosquito device, to capture and kill the mosquitos and insects effectively. The attractant is nontoxic, will not contaminate environment, and can be used in an indoor or outdoor area.

Description

Method for capturing mosquitoes and attractant device used in the method

Technical field

The present invention relates to a method of killing mosquitoes. In particular, the present invention relates to a mosquito trapping method capable of simultaneously absorbing an odor mainly by a bait, capable of simultaneously attracting male mosquitoes and female mosquitoes to a mosquito trap, and an attractant device used in the method.

Background technique

More than one million people worldwide die every year from mosquito-borne diseases such as malaria, dengue fever, encephalitis and West Nile virus infection. In addition to the possible diseases, mosquito bites often interfere with humans, causing discomfort to human body and sleep. The use of chemical insecticides to kill mosquitoes is harmful to human health and has environmentally damaging consequences. The same insecticides produce resistance to chemical insecticides. The use of mosquito coils and the like can only temporarily remove mosquitoes without reducing their quantity.

Female mosquitoes have a life cycle of at least one to two months. Most of them start to absorb animal blood for 2-3 days after emergence, develop ovaries, breed offspring and supply nutrients for fertilized eggs during pregnancy. At the same time, female mosquitoes are infected with viruses in biting animals. The relative male mosquitoes usually have a life cycle of 7-10 days. They can be mated two days after emergence. They only need to rely on water and leaf juice to survive, and will not attack animals and infectious viruses. Therefore, the study of most mosquito attractants is limited to female mosquitoes and the research on male mosquitoes is rarely found in the literature.

Nasci and 1983 pointed out that the common light trapping mosquitoes use ultraviolet light to attract mosquitoes, but they can only capture the photosynthetic male mosquitoes or insects that scrape the beneficial insects that transmit pollen, but the effect on female mosquitoes is not good. Aedes aegypti and Aedes aegypti, which attack humans during the day and infect dengue fever, are completely ineffective.

In the early twentieth century, biologists began to study the attraction of carbon dioxide and chemical odor to mosquitoes. Takken and Knold 1999; Allan et al. 2006; Cook et al. 2011; Kline et al. 2012; Have an in-depth discussion.

Bernier et al. (2000) analyzed human skin exudates using GC/MS instruments containing more than 300 compounds, including carboxylic acids, alcohols, aldehydes, ketones, aromatic hydrocarbons, amides, esters, halogenated hydrocarbons, and heterocyclic compounds. The main components of sweat are water, L-lactic acid, ammonia and urea. Many literatures have investigated the potential of different elements of biological respiration and skin discharge for different species of mosquitoes. Bosch et al., 1999 pointed out that the concentration of the attractant in the air is enough to attract mosquitoes in parts per million or less.

Carbon dioxide is one of the important elements that attract mosquitoes to target. A photocatalyst mosquito trap that has been known to be commercially used, which adds a titanium dioxide coating inside the mosquito trap, claiming that titanium dioxide can react with ultraviolet rays to generate negative ion oxygen, and negative ion oxygen chemically reacts with floating microorganisms in the air to form carbon dioxide and water. . The mosquito-trapping effect can be enhanced by the combination of ultraviolet light and carbon dioxide. However, no scientific instrument has detected the carbon dioxide produced by photocatalyst mosquito traps. The mosquito trapping effect of this mosquito trap is similar to that of a mosquito trap with only a light source.

The most representative of the bait-enhanced mosquito-repellent device is the mosquito-killing magnet produced by Woodstream in the United States. The combustion of propane or liquefied petroleum gas produces carbon dioxide as the main bait for attracting mosquitoes, and its concentration needs to reach above 500 PPM. The auxiliary bait is placed at the air outlet to be discharged with the main bait to enhance the efficiency of mosquito trapping. However, the cost of this product is high, and the average consumer has no ability to purchase; the safety considerations for the storage and transportation of propane or liquefied petroleum gas, and the high concentration of carbon dioxide emissions have limited the popularity of mosquito-killing magnetism.

Although bioscientists have extensively studied the attracting effects of different odors on mosquitoes, they are limited to one or a combination of odors for the academic discussion of female mosquitoes of certain mosquito species with less practical considerations. The invention extracts effective attracting elements from a plurality of attractants and can volatilize at room temperature to attract mosquitoes, and combines simple mosquito traps to effectively kill different mosquito species, male mosquitoes and female mosquitoes.

Reference patent documents:

1. Patent position: US patent of US 6,145,243;

2. The Chinese invention patent application with the patent application number: 200510135487.5;

3. The patent application number is: 201010142601.8 Chinese invention patent application.

Summary of the invention

The technical problem to be solved by the present invention lies in the comprehensive academic foundation of the above, and proposes a mosquito trapping method for various mosquito species and simultaneous male mosquitoes and female mosquitoes, and an attractant device used in the method.

In order to solve the above technical problem, the mosquito trapping method of the present invention adopts the following technical solution: the method is to install an attractant which can volatilize the odor attracting bait at the air inlet of the mosquito trap, and use the attractant to volatilize to form an odor bait. The mosquito is attracted to the area, and then the mosquito is sucked into the mosquito trap by the negative pressure airflow generated by the mosquito trap to form a mosquito for killing; wherein the attractant comprises three kinds of three separate spaces. Different substances that can be volatilized: lactic acid, octene alcohol, and compounds capable of cracking and releasing ammonia gas and carbon dioxide, respectively, which are volatilized outward through through holes provided in respective spaces, and constitute Forming an attractive scent bait to mosquitoes.

Further, in the above technical solution, the lactic acid and the octene alcohol are liquid, and are immersed in a carrier capable of adsorbing liquid, and the flowability of the lactic acid and the octene alcohol and the volatilization rate of the controller are reduced by the carrier; The enol volatilization rate is controlled at 0.1-10 mg/hr.

Further, in the above technical solution, the compound capable of cracking and releasing ammonia gas and carbon dioxide is solid ammonium hydrogencarbonate.

Further, in the above technical solution, the ammonium hydrogencarbonate adopts calcium carbonate as a binder, and forms a mass by pressurization to reduce the volatile surface area of ammonium hydrogencarbonate and control the volatilization rate; the ammonium hydrogencarbonate The volatilization rate is controlled at 1-300 mg / hr.

The technical solution adopted in the attractant device used in the mosquito trapping method of the present invention is that the device includes A bait box with three isolated spaces, three volatile different substances placed in three spaces: lactic acid, octene alcohol, and compounds capable of cracking and releasing ammonia and carbon dioxide. The three substances are volatilized outward through the through holes provided in the respective spaces, and constitute an odor bait which attracts mosquitoes to form an attraction.

The bait box is composed of a plastic cylinder body constituting three mutually isolated spaces, a tin foil paper covering the opening of the plastic cylinder body, and a plastic top cover, and the top cover is respectively opened corresponding to three mutually isolated spaces. There are through holes; after the three materials are placed in three spaces, the three spaces are sealed by the foil paper, and the foil is locked by the cooperation of the top cover and the opening of the cylinder; the sharp object passes through the top cover The through hole pierces the tin foil paper to cause the three substances to volatilize the odor bait.

In the attractant device of the present invention, a ternary bait, lactic acid, octene alcohol and ammonium hydrogencarbonate are respectively placed in three separate spaces of the bait box, and the three chemical components can be volatilized at room temperature under air flow conditions. Enough to attract mosquito concentration. The odor formed by the volatilization of the ternary bait is mixed, and the odor emitted by the human body is extremely similar, which will attract the formation of characters. Because the mosquito's olfactory sensation is acute, a trace of effective bait odor can effectively attract mosquitoes. The bait box of the present invention is placed in the center of the air inlet of the mosquito trap, and when the mosquito is attracted to the bait box accessory by a small amount of attractant, the mosquito trap is sucked into the mosquito trap.

The present invention has the following features with respect to the current conventional scheme:

1. The odor generated by the volatilization of the three substances in the attractant is similar to that produced by the human body, thereby misleading the mosquitoes, thereby attracting the mosquitoes, attracting them to the effective working area of the mosquito trap, and effectively killing the mosquitoes.

2. The three substances in the attractant of the present invention are volatile, non-toxic and do not pollute the environment, and are environmentally friendly, and can be used both outdoors and indoors.

3. Mosquitoes produce antibodies against insecticides, and the attractants of the present invention can effectively and effectively hunt mosquitoes.

DRAWINGS

1 is a schematic structural view of a first embodiment of an attractant device of the present invention;

Figure 2 is a schematic view showing the structure of a mosquito trap used in the present invention;

Figure 3 is a table showing the indoor capture effect of the 4 day old non-breathing Aedes albopictus;

Figure 4 is a table showing the indoor capture effect of the present invention for a 4 day old non-breathing Aedes aegypti;

Figure 5 is a table showing the indoor capture effect of the 5 day old non-breathing Aedes aegypti;

Figure 6 is a table showing the indoor capture effect of the 6 day old non-breathing Aedes aegypti;

Figure 7 is a data sheet of indoor capture effect of the 4 day old non-blooded Anopheles sinensis;

Fig. 8 is a table showing the indoor capture effect of the present invention for a 4-day-old non-hemorrhagic trichophyton.

detailed description

The test of the present invention was carried out by the Chinese Center for Disease Control and Prevention of the Chinese Center for Disease Control and Prevention, and tested for the Chinese high-infected Aedes aegypti (dengue and yellow fever), Aedes albopictus (dengue fever, Japanese encephalitis) , Chikungunya fever ), the attracting effect of Anopheles sinensis (malaria, except Qinghai Xinjiang) and Culex pipiens pallens (epidemic encephalitis and malaria).

1. Supply test insects:

Aedes albopictus, Anopheles sinensis and Culex pipiens pallens are adult mosquitoes four days after emergence, and Aedes aegypti is two days, three days, four days, five days and six days of adult mosquitoes after emergence. Each test requires about 200, male and female, and the female mosquito does not suck blood after emergence. The tested mosquito species were provided by the Media Biology Control Office of the Center for Infectious Disease Control and Prevention of the Chinese Center for Disease Control and Prevention.

2. Decoy concentration of bait and bait:

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1 is a schematic exploded perspective view of an attractant device of the present invention. The device comprises a bait box 20 having three mutually isolated spaces, three volatile different substances respectively placed in three spaces: lactic acid, octene alcohol, and capable of cracking and releasing ammonia gas. And the carbon dioxide compound, the three substances are volatilized outward through the small holes provided in the respective spaces, and constitute an odor bait which attracts mosquitoes to form an attraction.

The bait box 20 includes a barrel 201, a foil paper 202, and a top cover 203. The cylindrical body 201 and the top cover 203 are made of a plastic material, wherein three mutually isolated spaces are formed inside the cylindrical body 201, and the appropriate amounts of the above three substances are respectively placed in different spaces, and after the tubular body 201 is sealed by the tin foil paper 202, The top cover 203 is screwed to the port portion of the cylinder 201, thereby forming a gland on the foil paper 202, sealing the three mutually isolated spaces in the cylinder 201, and preventing the volatilization of the three substances by sealing the foil paper. . The top cover 203 is provided with a through hole 204. When the attracting device is used, the tin foil is pierced through a through hole 204 by a sharp object such as a needle or a thorn, and the three substances are volatilized outward through the through hole 204. A scent bait.

Among the three substances, ammonium hydrogencarbonate is a solid powder which is slowly decomposed into ammonia gas, carbon dioxide and water at room temperature. The ammonium hydrogencarbonate used in the present invention uses calcium carbonate as a binder and is extruded into a cake to reduce the volatile surface area and control the volatilization rate. The lactic acid and octenol used in the present invention are liquids which are immersed in a liquid-adsorbable carrier to reduce the fluidity thereof without affecting the volatilization rate thereof. The carrier capable of adsorbing the liquid may be a material having micropores such as non-woven fabric, sponge, or cotton wool.

The present invention controls the range of volatilization rates of three materials, from 50 to 300 mg/hr of ammonium bicarbonate and from 1 to 5 mg/hr of lactic acid and octenol.

3. The mosquito trap used in the case:

As shown in Fig. 2, the mosquito trap 10 used in the present invention is an exhaust type mosquito trap. It is similar to a fan except that the fan is blown outward, and the mosquito trap 10 used in the present invention is of a draft type, which will lure The agent device 20 is placed in the central region of the air inlet 401 of the mosquito trap 10. When the mosquito trap 10 is activated, the air flow increases the volatilization of the bait material in the attractant device, and when the mosquito is attracted to the mosquito trap 10 by the scent bait In port 401, the mosquito trap 10 generates a negative pressure to draw the mosquito into the mosquito trap.

As shown in FIG. 2, the trap 10 includes a light emitting device 30, a housing 40, a fan device 50, and a collecting device 60. The attractant device 20 is used to scent the scent bait to attract mosquitoes. The fan unit 50 is disposed in the above-described hollow portion of the housing 40 for generating an air flow from the air inlet 401 to the exhaust port 402 through the housing 40 to inhale mosquitoes from the air inlet 401. The collection device 60 is coupled to the exhaust port 402 of the housing 40 for collecting mosquitoes that are drawn into the housing 40 and exhausting airflow generated by the fan unit 50. In addition, the light generated by the illuminating device 30 has an effect of assisting in attracting mosquitoes in a dark environment.

4. Test conditions:

Evaluation of the capture effect of Aedes albopictus and Aedes aegypti, at a temperature of 25±1°C and a relative humidity of 50%±10%, natural light on sunny days; assessment of the capture effect of Anopheles sinensis and Culex pipiens pallens, at temperature 27±1°C, relative humidity 70%±10%, natural light for sunny days.

5. Determination method

Example 1: Aedes albopictus and Aedes aegypti

1 Place the mosquito net in a room controlled by temperature and humidity conditions, wherein the mosquito net size is 240 cm long, 220 cm wide and 175 cm high.

2 Place the mosquito trap to be tested on a table 30 cm from the ground in the middle of the mosquito net;

3 control room temperature is 24-26 ° C, relative humidity is 40-60%;

4 Put about 200 emerged Aedes mosquitoes into the mosquito nets around 21:00 on the night before the measurement, and the males and the females will make the mosquitoes free to move;

5 Start at 9:00 am and turn on the mosquito trap switch;

6 Every 15 minutes, 30 minutes, 60 minutes, 120 minutes, then every 2 hours, until 12 hours, record the number of mosquitoes in the trap, and identify the male and female; the test was repeated 3 times.

(2) Determination of Anopheles sinensis and Culex pipiens

3 control room temperature is 26-28 ° C, relative humidity is 50-70%;

4 At about 10:00 am on the day of the measurement, about 200 mosquitoes of the Anopheles sinensis and Culex pipiens pallens on the 4th day after emergence were placed in the mosquito net, and the males and the females were half-lived to make the mosquitoes free to move;

5 starts at 5:00 pm and turns on the mosquito trap switch;

6 The 15th minute, the 30th minute, the 60th minute, the 120th minute after the mosquito trap operation, and every 2 hours after the observation, respectively, and the number of mosquitoes of the trap was recorded until 12 hours (the three species of Culex pipiens) 8 hours, 10 hours without recording), and distinguish between male and female; the test was repeated 3 times.

Experimental results:

Example 1: Aedes albopictus and Aedes aegypti

The capture effect of the present invention on Aedes albopictus can be seen from Fig. 3, and has a good capturing effect on the 4-day-old Aedes albopictus, and the cumulative capture rate of female mosquitoes in 12 hours is 73.37%, and the capture of Aedes albopictus mosquitoes. The rate is as high as 96.89%.

Example 2: Capture effect of Aedes aegypti

The 12-hour capture effect of Aedes aegypti on the 4 days after emergence is not only 36.46±8.92% (see FIG. 4), but with the extension of the time after emergence to the 5th and 6th days, The 12-hour cumulative female mosquito capture rate increased to 79.10±5.83% (shown in Figure 5) and 90.45±6.32% (see figure 6)). The increase of the capture rate with the increase of the emergence time is related to the development of Aedes aegypti female mosquitoes. As the development of Aedes aegypti female mosquitoes matures, the capture efficiency of the present invention increases accordingly.

Example 3: Capture effect of Anopheles sinensis

The present invention is better for the 4-day-old non-blooded Anopheles sinensis, as shown in Fig. 7, the 12-hour cumulative female mosquito capture rate is 79.84±7.55%, which is similar to the cumulative capture rate of the 4-day-old non-blooded Aedes albopictus. .

Example 4: Capture effect of Culex pipiens pallens

As shown in FIG. 8 , the 12-hour cumulative female mosquito capture rate of the 4 day old non-hemorrhagic Culex pipiens pallens in the present invention is 51.08±5.45%, which is higher than the cumulative capture rate of the 4th day old non-blooded Aedes aegypti at the same time. The capture rate of Aedes albopictus and Anopheles sinensis under the same conditions.

The above description is only for the specific embodiment of the present invention, and is not intended to limit the scope of the present invention. The equivalent changes or modifications made by the structures, features and principles of the present invention should be included in Within the scope of the patent application of the present invention.

Claims

A mosquito trapping method, which is characterized in that: an attractant device for volatilizing an odor attracting bait is placed at an air inlet of a mosquito trap, and an odor bait formed by volatilization of an attractant is used to attract mosquitoes to the region. Then, the negative pressure air generated by the mosquito trap is used to suck the mosquito into the mosquito trap to form a mosquito to kill;

Wherein, the attractant comprises three volatile different substances respectively placed in three mutually isolated spaces: lactic acid, octene alcohol, and capable of cracking and releasing ammonia gas and carbon dioxide. The compound, the three substances are volatilized outward through the through holes provided in the respective spaces, and constitute an odor bait which forms an attraction to the mosquito.
A mosquito trapping method according to claim 1, wherein said lactic acid and octene alcohol are liquid, and are immersed in a liquid-adsorbable carrier to reduce the fluidity of lactic acid and octene alcohol through the carrier. And control its volatilization rate; lactic acid, octene alcohol volatilization rate is controlled at 0.1-10 mg / h.
A mosquito trapping method according to claim 1, wherein said compound capable of cracking and releasing ammonia gas and carbon dioxide is solid ammonium hydrogencarbonate.
A mosquito trapping method according to claim 3, wherein the ammonium hydrogencarbonate is formed by using calcium carbonate as a binder and forming a mass by pressurization to reduce the volatile surface area of ammonium hydrogencarbonate and control the volatilization. Rate; the ammonium bicarbonate volatilization rate is controlled at 1-300 mg / h.
An attractant device for use in a mosquito trapping method, characterized in that the device comprises a bait box having three mutually isolated spaces, three kinds of volatile different substances are respectively placed in three spaces, three kinds The substances are: lactic acid, octene alcohol, and a compound capable of cracking and releasing ammonia gas and carbon dioxide, respectively, which are volatilized outward through through holes provided in respective spaces, and constitute an odor bait which attracts mosquitoes to form an attraction.
An attractant device for use in a mosquito trapping method according to claim 5, wherein: The lactic acid and octene alcohol are liquid, and are immersed in a liquid-adsorbable carrier, and the flowability of the lactic acid and octenol and the controller volatilization rate are reduced by the carrier.
The attractant device for use in a mosquito trapping method according to claim 6, wherein the lactic acid and octene alcohol have a volatilization rate of 0.1 to 10 mg/hr.
The attractant device for use in a mosquito trapping method according to claim 5, wherein said compound capable of cracking and releasing ammonia gas and carbon dioxide is solid ammonium hydrogencarbonate; said ammonium hydrogencarbonate adopting Calcium carbonate is used as a binder to form a mass by pressurization to reduce the volatile surface area of ammonium bicarbonate and control the volatilization rate.
An attractant device for use in a mosquito trapping method according to claim 8, wherein said ammonium hydrogencarbonate volatilization rate is controlled at 1-300 mg/hr.
The attractant device for use in a mosquito trapping method according to any one of claims 5-9, characterized in that: the bait box is composed of a plastic cylinder constituting three mutually isolated spaces, covered with plastic The tin foil paper with the opening of the cylinder and the plastic top cover are combined, and the top cover is provided with three through holes respectively corresponding to the mutually isolated spaces; after the three materials are respectively placed in the three spaces, the tin foil paper will be The three spaces are sealed, and the foil is locked by the cooperation of the top cover and the opening of the cylinder; the tin foil is pierced by a sharp object passing through the through hole in the top cover to cause the three substances to volatilize the odor bait.